Status
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Gene information
General information
TC009441 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1187705..1193724
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009441
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01554

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009441 RA
      MADEHSSED SDNDNQEEL AGVLSKWTN YIHGWQERY 
      IVLKNGTLS YYKSEQESG YGCRGALSL AKAVIKPHE 
      FDECRFDVS VNDCVWYLR ADSVEAKQI WVDALESYK 
      LESGYGTGS ENSLKRHGS TVSLQSNTY STASGNSFK 
      RSSRNLREK LAELDTFKD ILCRQIETL QEYFDYCVD 
      NNEAQNVQN KEKLPAIDF KGEAITFKT TTKAVLDTL 
      EHCADLVAQ REESWRCRL EKEIERRKR SENLAKSYF 
      AQLQKSRVV HPGPDLEEG PHSVLADDE FYDAVESGL 
      DKMEEEQEL RERLKSGQV IPVTPPPTS PATLHRLWP 
      EIEKVVKQE VAMAKLGVG ESGTGWQLF AEDGEMRMY 
      RREEEVDGM VVDPLKAVH VVKGITGRE LCHYFFNPQ 
      YRYDWETTL EHMNVLETI SEDTLIFHQ THKRIWPAS 
      QRDVVFWSH LRRLPNDQD RDGPDIWTV VNNSTEHPD 
      HPANAGKCV RIFLTVCLL CQTRVDPPK AGAPVSRDD 
      ITCKITYCS VVNPGGWAP ASVLRAVYK REYPKFLKR 
      FTAYVKNQT KDKPIMY
    CDS sequence
    • >TC009441 RA
      atggccgac gaacacagc agcgaagac tccgacaac 
      gacaaccaa gaggaattg gcaggtgtg ttgagtaaa 
      tggactaat tatatacac ggatggcag gagcgttat 
      atcgttttg aaaaatgga actttatcc tattataaa 
      agcgagcag gaaagcggg tacggctgt agaggagct 
      ttatcgctt gccaaagca gttatcaaa ccccatgag 
      tttgacgag tgcaggttc gatgtctca gtcaatgac 
      tgcgtctgg tatttgcgg gccgactcg gttgaggcc 
      aaacaaatc tgggttgat gcgctagaa tcgtacaag 
      ctggagtct ggctatggt accggctcc gaaaacagc 
      ttgaagcgg catggctcc accgtcagc ctccagtca 
      aacacctac agcacagcc tctggaaac agcttcaaa 
      cgatcgtct cgcaacttg cgcgaaaaa ctcgccgaa 
      ttggacact ttcaaagat atcctatgc cgacaaatt 
      gagactttg caggaatat ttcgactat tgtgtggat 
      aataatgaa gcacaaaac gtacaaaac aaagaaaaa 
      ttgccagca attgatttt aaaggtgaa gcgataacg 
      ttcaagact acgaccaag gctgttttg gacactttg 
      gagcactgt gccgatttg gtggcacag agggaagag 
      tcatggcgt tgcagactc gagaaggaa atcgaacgg 
      aggaaaagg agtgaaaat ctggccaaa agctacttt 
      gcgcagctt caaaagtcc agagttgtg catccaggc 
      ccagatttg gaggaaggc ccccacagt gtgctggcc 
      gacgacgaa ttttacgat gccgtcgaa tccggtcta 
      gataagatg gaagaggag caagagttg agggaaaga 
      ctgaaaagc ggccaagtt attcccgtc actccgcct 
      ccgacatca ccagcgacc ttgcatcgt ttatggcct 
      gaaattgag aaagtggtg aagcaagaa gttgcgatg 
      gctaagtta ggtgtaggc gaatctggc actggatgg 
      cagttgttt gcagaagat ggcgagatg cgaatgtat 
      cggagggaa gaagaagtc gacggaatg gtcgtcgac 
      cctctcaag gctgtgcat gtggttaag gggatcacg 
      ggacgcgaa ctgtgtcac tactttttc aacccgcag 
      tacagatac gactgggaa accacattg gaacacatg 
      aacgtgctc gaaaccata tctgaggat actctgatc 
      tttcaccaa acacacaaa agaatctgg cctgcgagt 
      cagcgcgac gttgtgttt tggtcgcat ttgcgccga 
      ttgcccaac gatcaggac agagacggt cctgatatt 
      tggacggtg gttaacaat tccactgag catcctgat 
      catcctgcg aatgcgggt aagtgtgtg cgaatattt 
      ctgacagtg tgtctgtta tgtcaaacg cgagttgat 
      ccaccaaaa gctggagca ccggttagt cgagacgac 
      ataacgtgt aaaataact tattgttct gtcgtgaat 
      cccggaggg tgggccccc gcgtcggtg ctgagagcc 
      gtttataaa cgagaatat ccgaaattt ttgaagagg 
      tttaccgct tatgttaaa aaccaaaca aaagataag 
      cctattatg tactaa
    mRNA sequence
    • >TC009441 RA
      tgtgatttg agtgccgct tcggtttgt gtcgtgata 
      aaaacaaat tgataacgc acaatcatc ccaatgcca 
      tttttgtaa tgtgtcgca tgatatatt tacgggatt 
      ccctcgctt gaaatgact tgaatcctc gtgaccaca 
      ccacaacac acatcgacc atccgcaat tagaatggc 
      cgacgaaca cagcagcga agactccga caacgacaa 
      ccaagagga attggcagg tgtgttgag taaatggac 
      taattatat acacggatg gcaggagcg ttatatcgt 
      tttgaaaaa tggaacttt atcctatta taaaagcga 
      gcaggaaag cgggtacgg ctgtagagg agctttatc 
      gcttgccaa agcagttat caaacccca tgagtttga 
      cgagtgcag gttcgatgt ctcagtcaa tgactgcgt 
      ctggtattt gcgggccga ctcggttga ggccaaaca 
      aatctgggt tgatgcgct agaatcgta caagctgga 
      gtctggcta tggtaccgg ctccgaaaa cagcttgaa 
      gcggcatgg ctccaccgt cagcctcca gtcaaacac 
      ctacagcac agcctctgg aaacagctt caaacgatc 
      gtctcgcaa cttgcgcga aaaactcgc cgaattgga 
      cactttcaa agatatcct atgccgaca aattgagac 
      tttgcagga atatttcga ctattgtgt ggataataa 
      tgaagcaca aaacgtaca aaacaaaga aaaattgcc 
      agcaattga ttttaaagg tgaagcgat aacgttcaa 
      gactacgac caaggctgt tttggacac tttggagca 
      ctgtgccga tttggtggc acagaggga agagtcatg 
      gcgttgcag actcgagaa ggaaatcga acggaggaa 
      aaggagtga aaatctggc caaaagcta ctttgcgca 
      gcttcaaaa gtccagagt tgtgcatcc aggcccaga 
      tttggagga aggccccca cagtgtgct ggccgacga 
      cgaatttta cgatgccgt cgaatccgg tctagataa 
      gatggaaga ggagcaaga gttgaggga aagactgaa 
      aagcggcca agttattcc cgtcactcc gcctccgac 
      atcaccagc gaccttgca tcgtttatg gcctgaaat 
      tgagaaagt ggtgaagca agaagttgc gatggctaa 
      gttaggtgt aggcgaatc tggcactgg atggcagtt 
      gtttgcaga agatggcga gatgcgaat gtatcggag 
      ggaagaaga agtcgacgg aatggtcgt cgaccctct 
      caaggctgt gcatgtggt taaggggat cacgggacg 
      cgaactgtg tcactactt tttcaaccc gcagtacag 
      atacgactg ggaaaccac attggaaca catgaacgt 
      gctcgaaac catatctga ggatactct gatctttca 
      ccaaacaca caaaagaat ctggcctgc gagtcagcg 
      cgacgttgt gttttggtc gcatttgcg ccgattgcc 
      caacgatca ggacagaga cggtcctga tatttggac 
      ggtggttaa caattccac tgagcatcc tgatcatcc 
      tgcgaatgc gggtaagtg tgtgcgaat atttctgac 
      agtgtgtct gttatgtca aacgcgagt tgatccacc 
      aaaagctgg agcaccggt tagtcgaga cgacataac 
      gtgtaaaat aacttattg ttctgtcgt gaatcccgg 
      agggtgggc ccccgcgtc ggtgctgag agccgttta 
      taaacgaga atatccgaa atttttgaa gaggtttac 
      cgcttatgt taaaaacca aacaaaaga taagcctat 
      tatgtacta aagcataag atcttttta tggtttaca 
      ttgttaatt ttatttata tttcgtata ttattaaat 
      gttatcttc tgttgtttt ttgttttat cttttcttt 
      tgggtgttc gactcctaa aaccgtccc taactagtt 
      gtgcttttt ggaggattt tgttaaccg ttttgattg 
      ttttttgca tatgtgtat aaaatattg tacatttaa 
      atgttgaag actgcttaa aattgttga gaccagttg 
      ccttgatag tgtgaaaaa taattgtat aacttaaat 
      ttatgcttt gcatttctg taaattgaa aaatgtact 
      ctgattgta agatacaag aaacgatgt tttcgaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY