General information | |||
TC009431 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 1325278..1327254 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC009431 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01551 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
remarks: muscle pattern potentially obscured by segmentation defect,
head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
procephalic head mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC009431 RA
MERIYVSSI GAFILYTLS TVALAKPFE DEKSFSDDN KLHCVDSND VGLNNERSN PNSFAEFYK PRSRFEGVE KFANRPFDF DTKRSAEES PVNSLEKRQ VGVVYKDEM PIGILQPYQ RSYRFIPIK EQSPANLDA SQAQDVPYS VPSFADIDK REVKELDET YPSILTNFK IVNKLEKKR NYDDKNVRD LKATIRRLR SANPHNNEK IEEELKNIL DDMGLIEDE ESHGVKREA SDDSLVEEE LVAENERNK RDDNCNNNP NSKTTYDHN PASDNNQRN ELKTENHPN VYKRESSDE SERRKRQKS ELVENLKDS GELMVSGNK SPLASSEIT NDKRSSEEE EDYESRIER NIQNKINSL KEEVKREIE ALRKKQDDE DDDEEDYQR KKRQVYDTL MNEETDQVN PAMNPDNEL KPLVRKRRQ VDGGKRLER SSDARPQEE AEREKSEEG GELLGDFNE GAVAKREVY SPDQEYDDG LYDRNKRDY DSFFSDYYY NNRAKRQNR FVPMRGNRW RRFRRRINE DQDLFGALP KSYDGELSR YKRVKRK
- >TC009431 RB
MERIYVSSI GAFILYTLS TVALAKPFE DEKSFSDDN KLHCVDSND VGLNNERSN PNSFAEFYK PRSRFEGVE KFANRPFDF DTKRSAEES PVNSLEKRQ VGVVYKDEM PIGILQPYQ RSYRFIPIK EQSPANLDA SQAQDVPYS VPSFADIDK REVKELDET YPSILTNFK IVNKLEKKR NYDDKNVRD LKATIRRLR SANPHNNEK IEEELKNIL DDMGLIEDE ESHGVKREA SDDSLVEEE LVAENERNK RDDNCNNNP NSKTTYDHN PASDNNQRN ELKTENHPN VYKRESSDE SERRKRQKS ELVENLKDS GELMVSGNK SPLASSEIT NDKRSSEEE EDYESRIER NIQNKINSL KEEVKREIE ALRKKQDDE DDDEEDYQR KKRQVYDTL MNEETDQVN PAMNPDNEL KPLVRKRRQ VDGGKRLER SSDARPQEE AEREKSEEG GELLGDFNE GAVAKREVY SPDQEYDDG LYDRNKRDY DSFFSDYYY NNRAKRQNR FVPMRGNRW RRFRRRINE DQGMGNRPQ QLSDMSDLD LFGALPKSY DGELSRYKR VKRK
- >TC009431 RA
atggaaaga atttatgtc agcagcatt ggggcattc attctatat acactcagt actgtagcg cttgcaaaa cccttcgag gacgagaaa agtttcagt gatgacaac aaattacac tgtgtggac tcaaacgac gtcggcctg aacaatgag cggagcaac cccaactca tttgccgag ttctacaaa cctcgctca agattcgaa ggggttgag aagttcgca aaccggccg tttgacttt gacacgaaa cgatccgca gaggaatcg cccgtcaac tcgctggag aagcggcaa gttggggtg gtgtacaaa gatgagatg ccaattggt atattacaa ccttatcaa cgctcttac cgctttata ccaatcaag gaacaaagt ccagctaat ctcgatgct agtcaggct caggatgtt ccttacagt gtacccagt tttgcagat attgacaaa agggaagtc aaagagctg gatgaaacc tacccttcc atcctgaca aattttaaa attgtgaac aaactcgag aaaaaacga aattacgat gacaaaaac gttagggat ttgaaagca acaattcgc cgcttgcgt tccgcaaac ccccacaac aacgagaaa attgaagaa gaattgaag aatatcctg gacgatatg gggctcatt gaagatgaa gaaagccac ggtgtgaag cgtgaagcc agcgatgat tcgttggtc gaggaagaa ttggttgca gaaaacgaa cggaacaaa agggatgat aattgtaat aataacccc aacagtaag acaacctac gaccacaac cctgccagt gataataat caaaggaac gaactcaaa actgaaaac catccaaat gtgtacaag agagagtca agtgacgag tccgagcgc cgaaaacgg caaaaaagc gaacttgtc gaaaatctg aaagactca ggcgagttg atggtcagt gggaacaag tcacccttg gcaagttcc gaaatcacg aatgataag cgatcctcc gaagaggag gaagattac gagtcgagg atcgagcgc aacatccag aacaaaatc aactcgctg aaggaagaa gtgaagcgg gaaatcgaa gctttgcgg aagaagcaa gacgatgaa gacgacgac gaggaggat taccaaagg aagaagcgc caagtgtac gacactttg atgaacgaa gaaaccgac caagtgaac ccggccatg aatccagac aatgagctt aaaccgctt gtcaggaaa cggagacaa gtcgatggg gggaaaaga ctggagcgg tcttccgac gctcgcccg caggaggag gccgagcgg gagaaaagt gaagaaggg ggtgaattg ctgggggat tttaacgaa ggagcggtc gcaaaacgg gaggtttac agtccggat caggaatat gatgacggg ttgtatgac aggaataag cgggattat gacagtttt ttttctgat tattattac aataatcgg gcgaagagg caaaatcgc tttgtaccg atgagggga aatcggtgg aggaggttt aggaggagg attaatgaa gatcaagat ctttttgga gcgttgccg aaaagttac gatggggag ttgtcaagg tataagcgt gtcaaacgg aaatga
- >TC009431 RB
atggaaaga atttatgtc agcagcatt ggggcattc attctatat acactcagt actgtagcg cttgcaaaa cccttcgag gacgagaaa agtttcagt gatgacaac aaattacac tgtgtggac tcaaacgac gtcggcctg aacaatgag cggagcaac cccaactca tttgccgag ttctacaaa cctcgctca agattcgaa ggggttgag aagttcgca aaccggccg tttgacttt gacacgaaa cgatccgca gaggaatcg cccgtcaac tcgctggag aagcggcaa gttggggtg gtgtacaaa gatgagatg ccaattggt atattacaa ccttatcaa cgctcttac cgctttata ccaatcaag gaacaaagt ccagctaat ctcgatgct agtcaggct caggatgtt ccttacagt gtacccagt tttgcagat attgacaaa agggaagtc aaagagctg gatgaaacc tacccttcc atcctgaca aattttaaa attgtgaac aaactcgag aaaaaacga aattacgat gacaaaaac gttagggat ttgaaagca acaattcgc cgcttgcgt tccgcaaac ccccacaac aacgagaaa attgaagaa gaattgaag aatatcctg gacgatatg gggctcatt gaagatgaa gaaagccac ggtgtgaag cgtgaagcc agcgatgat tcgttggtc gaggaagaa ttggttgca gaaaacgaa cggaacaaa agggatgat aattgtaat aataacccc aacagtaag acaacctac gaccacaac cctgccagt gataataat caaaggaac gaactcaaa actgaaaac catccaaat gtgtacaag agagagtca agtgacgag tccgagcgc cgaaaacgg caaaaaagc gaacttgtc gaaaatctg aaagactca ggcgagttg atggtcagt gggaacaag tcacccttg gcaagttcc gaaatcacg aatgataag cgatcctcc gaagaggag gaagattac gagtcgagg atcgagcgc aacatccag aacaaaatc aactcgctg aaggaagaa gtgaagcgg gaaatcgaa gctttgcgg aagaagcaa gacgatgaa gacgacgac gaggaggat taccaaagg aagaagcgc caagtgtac gacactttg atgaacgaa gaaaccgac caagtgaac ccggccatg aatccagac aatgagctt aaaccgctt gtcaggaaa cggagacaa gtcgatggg gggaaaaga ctggagcgg tcttccgac gctcgcccg caggaggag gccgagcgg gagaaaagt gaagaaggg ggtgaattg ctgggggat tttaacgaa ggagcggtc gcaaaacgg gaggtttac agtccggat caggaatat gatgacggg ttgtatgac aggaataag cgggattat gacagtttt ttttctgat tattattac aataatcgg gcgaagagg caaaatcgc tttgtaccg atgagggga aatcggtgg aggaggttt aggaggagg attaatgaa gatcaaggt atgggtaat aggccacaa cagctcagt gatatgtct gatttagat ctttttgga gcgttgccg aaaagttac gatggggag ttgtcaagg tataagcgt gtcaaacgg aaatga
- >TC009431 RA
gaaaatggc acaaatttc cgtttgttg ccgtttttt gatttttac atcaatgtg acatcagtc gcatttatt tttttgtac tcaactgca tattatgga aagaattta tgtcagcag cattggggc attcattct atatacact cagtactgt agcgcttgc aaaaccctt cgaggacga gaaaagttt cagtgatga caacaaatt acactgtgt ggactcaaa cgacgtcgg cctgaacaa tgagcggag caaccccaa ctcatttgc cgagttcta caaacctcg ctcaagatt cgaaggggt tgagaagtt cgcaaaccg gccgtttga ctttgacac gaaacgatc cgcagagga atcgcccgt caactcgct ggagaagcg gcaagttgg ggtggtgta caaagatga gatgccaat tggtatatt acaacctta tcaacgctc ttaccgctt tataccaat caaggaaca aagtccagc taatctcga tgctagtca ggctcagga tgttcctta cagtgtacc cagttttgc agatattga caaaaggga agtcaaaga gctggatga aacctaccc ttccatcct gacaaattt taaaattgt gaacaaact cgagaaaaa acgaaatta cgatgacaa aaacgttag ggatttgaa agcaacaat tcgccgctt gcgttccgc aaaccccca caacaacga gaaaattga agaagaatt gaagaatat cctggacga tatggggct cattgaaga tgaagaaag ccacggtgt gaagcgtga agccagcga tgattcgtt ggtcgagga agaattggt tgcagaaaa cgaacggaa caaaaggga tgataattg taataataa ccccaacag taagacaac ctacgacca caaccctgc cagtgataa taatcaaag gaacgaact caaaactga aaaccatcc aaatgtgta caagagaga gtcaagtga cgagtccga gcgccgaaa acggcaaaa aagcgaact tgtcgaaaa tctgaaaga ctcaggcga gttgatggt cagtgggaa caagtcacc cttggcaag ttccgaaat cacgaatga taagcgatc ctccgaaga ggaggaaga ttacgagtc gaggatcga gcgcaacat ccagaacaa aatcaactc gctgaagga agaagtgaa gcgggaaat cgaagcttt gcggaagaa gcaagacga tgaagacga cgacgagga ggattacca aaggaagaa gcgccaagt gtacgacac tttgatgaa cgaagaaac cgaccaagt gaacccggc catgaatcc agacaatga gcttaaacc gcttgtcag gaaacggag acaagtcga tggggggaa aagactgga gcggtcttc cgacgctcg cccgcagga ggaggccga gcgggagaa aagtgaaga agggggtga attgctggg ggattttaa cgaaggagc ggtcgcaaa acgggaggt ttacagtcc ggatcagga atatgatga cgggttgta tgacaggaa taagcggga ttatgacag ttttttttc tgattatta ttacaataa tcgggcgaa gaggcaaaa tcgctttgt accgatgag gggaaatcg gtggaggag gtttaggag gaggattaa tgaagatca agatctttt tggagcgtt gccgaaaag ttacgatgg ggagttgtc aaggtataa gcgtgtcaa acggaaatg agttgtatt ttttaatgt attttttat taaatatat caactatta
- >TC009431 RB
gaaaatggc acaaatttc cgtttgttg ccgtttttt gatttttac atcaatgtg acatcagtc gcatttatt tttttgtac tcaactgca tattatgga aagaattta tgtcagcag cattggggc attcattct atatacact cagtactgt agcgcttgc aaaaccctt cgaggacga gaaaagttt cagtgatga caacaaatt acactgtgt ggactcaaa cgacgtcgg cctgaacaa tgagcggag caaccccaa ctcatttgc cgagttcta caaacctcg ctcaagatt cgaaggggt tgagaagtt cgcaaaccg gccgtttga ctttgacac gaaacgatc cgcagagga atcgcccgt caactcgct ggagaagcg gcaagttgg ggtggtgta caaagatga gatgccaat tggtatatt acaacctta tcaacgctc ttaccgctt tataccaat caaggaaca aagtccagc taatctcga tgctagtca ggctcagga tgttcctta cagtgtacc cagttttgc agatattga caaaaggga agtcaaaga gctggatga aacctaccc ttccatcct gacaaattt taaaattgt gaacaaact cgagaaaaa acgaaatta cgatgacaa aaacgttag ggatttgaa agcaacaat tcgccgctt gcgttccgc aaaccccca caacaacga gaaaattga agaagaatt gaagaatat cctggacga tatggggct cattgaaga tgaagaaag ccacggtgt gaagcgtga agccagcga tgattcgtt ggtcgagga agaattggt tgcagaaaa cgaacggaa caaaaggga tgataattg taataataa ccccaacag taagacaac ctacgacca caaccctgc cagtgataa taatcaaag gaacgaact caaaactga aaaccatcc aaatgtgta caagagaga gtcaagtga cgagtccga gcgccgaaa acggcaaaa aagcgaact tgtcgaaaa tctgaaaga ctcaggcga gttgatggt cagtgggaa caagtcacc cttggcaag ttccgaaat cacgaatga taagcgatc ctccgaaga ggaggaaga ttacgagtc gaggatcga gcgcaacat ccagaacaa aatcaactc gctgaagga agaagtgaa gcgggaaat cgaagcttt gcggaagaa gcaagacga tgaagacga cgacgagga ggattacca aaggaagaa gcgccaagt gtacgacac tttgatgaa cgaagaaac cgaccaagt gaacccggc catgaatcc agacaatga gcttaaacc gcttgtcag gaaacggag acaagtcga tggggggaa aagactgga gcggtcttc cgacgctcg cccgcagga ggaggccga gcgggagaa aagtgaaga agggggtga attgctggg ggattttaa cgaaggagc ggtcgcaaa acgggaggt ttacagtcc ggatcagga atatgatga cgggttgta tgacaggaa taagcggga ttatgacag ttttttttc tgattatta ttacaataa tcgggcgaa gaggcaaaa tcgctttgt accgatgag gggaaatcg gtggaggag gtttaggag gaggattaa tgaagatca aggtatggg taataggcc acaacagct cagtgatat gtctgattt agatctttt tggagcgtt gccgaaaag ttacgatgg ggagttgtc aaggtataa gcgtgtcaa acggaaatg agttgtatt ttttaatgt attttttat taaatatat caactatta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
