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Gene information
General information
TC009431 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1325278..1327254
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC009431
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes

      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_01551

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection

      Analysis of embryonic musculature and early development
      larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      procephalic head mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC009431 RA
        MERIYVSSI GAFILYTLS TVALAKPFE DEKSFSDDN 
KLHCVDSND VGLNNERSN PNSFAEFYK PRSRFEGVE 
KFANRPFDF DTKRSAEES PVNSLEKRQ VGVVYKDEM 
PIGILQPYQ RSYRFIPIK EQSPANLDA SQAQDVPYS 
VPSFADIDK REVKELDET YPSILTNFK IVNKLEKKR 
NYDDKNVRD LKATIRRLR SANPHNNEK IEEELKNIL 
DDMGLIEDE ESHGVKREA SDDSLVEEE LVAENERNK 
RDDNCNNNP NSKTTYDHN PASDNNQRN ELKTENHPN 
VYKRESSDE SERRKRQKS ELVENLKDS GELMVSGNK 
SPLASSEIT NDKRSSEEE EDYESRIER NIQNKINSL 
KEEVKREIE ALRKKQDDE DDDEEDYQR KKRQVYDTL 
MNEETDQVN PAMNPDNEL KPLVRKRRQ VDGGKRLER 
SSDARPQEE AEREKSEEG GELLGDFNE GAVAKREVY 
SPDQEYDDG LYDRNKRDY DSFFSDYYY NNRAKRQNR 
FVPMRGNRW RRFRRRINE DQDLFGALP KSYDGELSR 
YKRVKRK
      • >TC009431 RB
        MERIYVSSI GAFILYTLS TVALAKPFE DEKSFSDDN 
KLHCVDSND VGLNNERSN PNSFAEFYK PRSRFEGVE 
KFANRPFDF DTKRSAEES PVNSLEKRQ VGVVYKDEM 
PIGILQPYQ RSYRFIPIK EQSPANLDA SQAQDVPYS 
VPSFADIDK REVKELDET YPSILTNFK IVNKLEKKR 
NYDDKNVRD LKATIRRLR SANPHNNEK IEEELKNIL 
DDMGLIEDE ESHGVKREA SDDSLVEEE LVAENERNK 
RDDNCNNNP NSKTTYDHN PASDNNQRN ELKTENHPN 
VYKRESSDE SERRKRQKS ELVENLKDS GELMVSGNK 
SPLASSEIT NDKRSSEEE EDYESRIER NIQNKINSL 
KEEVKREIE ALRKKQDDE DDDEEDYQR KKRQVYDTL 
MNEETDQVN PAMNPDNEL KPLVRKRRQ VDGGKRLER 
SSDARPQEE AEREKSEEG GELLGDFNE GAVAKREVY 
SPDQEYDDG LYDRNKRDY DSFFSDYYY NNRAKRQNR 
FVPMRGNRW RRFRRRINE DQGMGNRPQ QLSDMSDLD 
LFGALPKSY DGELSRYKR VKRK
      CDS sequence
      • >TC009431 RA
        atggaaaga atttatgtc agcagcatt ggggcattc 
attctatat acactcagt actgtagcg cttgcaaaa 
cccttcgag gacgagaaa agtttcagt gatgacaac 
aaattacac tgtgtggac tcaaacgac gtcggcctg 
aacaatgag cggagcaac cccaactca tttgccgag 
ttctacaaa cctcgctca agattcgaa ggggttgag 
aagttcgca aaccggccg tttgacttt gacacgaaa 
cgatccgca gaggaatcg cccgtcaac tcgctggag 
aagcggcaa gttggggtg gtgtacaaa gatgagatg 
ccaattggt atattacaa ccttatcaa cgctcttac 
cgctttata ccaatcaag gaacaaagt ccagctaat 
ctcgatgct agtcaggct caggatgtt ccttacagt 
gtacccagt tttgcagat attgacaaa agggaagtc 
aaagagctg gatgaaacc tacccttcc atcctgaca 
aattttaaa attgtgaac aaactcgag aaaaaacga 
aattacgat gacaaaaac gttagggat ttgaaagca 
acaattcgc cgcttgcgt tccgcaaac ccccacaac 
aacgagaaa attgaagaa gaattgaag aatatcctg 
gacgatatg gggctcatt gaagatgaa gaaagccac 
ggtgtgaag cgtgaagcc agcgatgat tcgttggtc 
gaggaagaa ttggttgca gaaaacgaa cggaacaaa 
agggatgat aattgtaat aataacccc aacagtaag 
acaacctac gaccacaac cctgccagt gataataat 
caaaggaac gaactcaaa actgaaaac catccaaat 
gtgtacaag agagagtca agtgacgag tccgagcgc 
cgaaaacgg caaaaaagc gaacttgtc gaaaatctg 
aaagactca ggcgagttg atggtcagt gggaacaag 
tcacccttg gcaagttcc gaaatcacg aatgataag 
cgatcctcc gaagaggag gaagattac gagtcgagg 
atcgagcgc aacatccag aacaaaatc aactcgctg 
aaggaagaa gtgaagcgg gaaatcgaa gctttgcgg 
aagaagcaa gacgatgaa gacgacgac gaggaggat 
taccaaagg aagaagcgc caagtgtac gacactttg 
atgaacgaa gaaaccgac caagtgaac ccggccatg 
aatccagac aatgagctt aaaccgctt gtcaggaaa 
cggagacaa gtcgatggg gggaaaaga ctggagcgg 
tcttccgac gctcgcccg caggaggag gccgagcgg 
gagaaaagt gaagaaggg ggtgaattg ctgggggat 
tttaacgaa ggagcggtc gcaaaacgg gaggtttac 
agtccggat caggaatat gatgacggg ttgtatgac 
aggaataag cgggattat gacagtttt ttttctgat 
tattattac aataatcgg gcgaagagg caaaatcgc 
tttgtaccg atgagggga aatcggtgg aggaggttt 
aggaggagg attaatgaa gatcaagat ctttttgga 
gcgttgccg aaaagttac gatggggag ttgtcaagg 
tataagcgt gtcaaacgg aaatga
      • >TC009431 RB
        atggaaaga atttatgtc agcagcatt ggggcattc 
attctatat acactcagt actgtagcg cttgcaaaa 
cccttcgag gacgagaaa agtttcagt gatgacaac 
aaattacac tgtgtggac tcaaacgac gtcggcctg 
aacaatgag cggagcaac cccaactca tttgccgag 
ttctacaaa cctcgctca agattcgaa ggggttgag 
aagttcgca aaccggccg tttgacttt gacacgaaa 
cgatccgca gaggaatcg cccgtcaac tcgctggag 
aagcggcaa gttggggtg gtgtacaaa gatgagatg 
ccaattggt atattacaa ccttatcaa cgctcttac 
cgctttata ccaatcaag gaacaaagt ccagctaat 
ctcgatgct agtcaggct caggatgtt ccttacagt 
gtacccagt tttgcagat attgacaaa agggaagtc 
aaagagctg gatgaaacc tacccttcc atcctgaca 
aattttaaa attgtgaac aaactcgag aaaaaacga 
aattacgat gacaaaaac gttagggat ttgaaagca 
acaattcgc cgcttgcgt tccgcaaac ccccacaac 
aacgagaaa attgaagaa gaattgaag aatatcctg 
gacgatatg gggctcatt gaagatgaa gaaagccac 
ggtgtgaag cgtgaagcc agcgatgat tcgttggtc 
gaggaagaa ttggttgca gaaaacgaa cggaacaaa 
agggatgat aattgtaat aataacccc aacagtaag 
acaacctac gaccacaac cctgccagt gataataat 
caaaggaac gaactcaaa actgaaaac catccaaat 
gtgtacaag agagagtca agtgacgag tccgagcgc 
cgaaaacgg caaaaaagc gaacttgtc gaaaatctg 
aaagactca ggcgagttg atggtcagt gggaacaag 
tcacccttg gcaagttcc gaaatcacg aatgataag 
cgatcctcc gaagaggag gaagattac gagtcgagg 
atcgagcgc aacatccag aacaaaatc aactcgctg 
aaggaagaa gtgaagcgg gaaatcgaa gctttgcgg 
aagaagcaa gacgatgaa gacgacgac gaggaggat 
taccaaagg aagaagcgc caagtgtac gacactttg 
atgaacgaa gaaaccgac caagtgaac ccggccatg 
aatccagac aatgagctt aaaccgctt gtcaggaaa 
cggagacaa gtcgatggg gggaaaaga ctggagcgg 
tcttccgac gctcgcccg caggaggag gccgagcgg 
gagaaaagt gaagaaggg ggtgaattg ctgggggat 
tttaacgaa ggagcggtc gcaaaacgg gaggtttac 
agtccggat caggaatat gatgacggg ttgtatgac 
aggaataag cgggattat gacagtttt ttttctgat 
tattattac aataatcgg gcgaagagg caaaatcgc 
tttgtaccg atgagggga aatcggtgg aggaggttt 
aggaggagg attaatgaa gatcaaggt atgggtaat 
aggccacaa cagctcagt gatatgtct gatttagat 
ctttttgga gcgttgccg aaaagttac gatggggag 
ttgtcaagg tataagcgt gtcaaacgg aaatga
      mRNA sequence
      • >TC009431 RA
        gaaaatggc acaaatttc cgtttgttg ccgtttttt 
gatttttac atcaatgtg acatcagtc gcatttatt 
tttttgtac tcaactgca tattatgga aagaattta 
tgtcagcag cattggggc attcattct atatacact 
cagtactgt agcgcttgc aaaaccctt cgaggacga 
gaaaagttt cagtgatga caacaaatt acactgtgt 
ggactcaaa cgacgtcgg cctgaacaa tgagcggag 
caaccccaa ctcatttgc cgagttcta caaacctcg 
ctcaagatt cgaaggggt tgagaagtt cgcaaaccg 
gccgtttga ctttgacac gaaacgatc cgcagagga 
atcgcccgt caactcgct ggagaagcg gcaagttgg 
ggtggtgta caaagatga gatgccaat tggtatatt 
acaacctta tcaacgctc ttaccgctt tataccaat 
caaggaaca aagtccagc taatctcga tgctagtca 
ggctcagga tgttcctta cagtgtacc cagttttgc 
agatattga caaaaggga agtcaaaga gctggatga 
aacctaccc ttccatcct gacaaattt taaaattgt 
gaacaaact cgagaaaaa acgaaatta cgatgacaa 
aaacgttag ggatttgaa agcaacaat tcgccgctt 
gcgttccgc aaaccccca caacaacga gaaaattga 
agaagaatt gaagaatat cctggacga tatggggct 
cattgaaga tgaagaaag ccacggtgt gaagcgtga 
agccagcga tgattcgtt ggtcgagga agaattggt 
tgcagaaaa cgaacggaa caaaaggga tgataattg 
taataataa ccccaacag taagacaac ctacgacca 
caaccctgc cagtgataa taatcaaag gaacgaact 
caaaactga aaaccatcc aaatgtgta caagagaga 
gtcaagtga cgagtccga gcgccgaaa acggcaaaa 
aagcgaact tgtcgaaaa tctgaaaga ctcaggcga 
gttgatggt cagtgggaa caagtcacc cttggcaag 
ttccgaaat cacgaatga taagcgatc ctccgaaga 
ggaggaaga ttacgagtc gaggatcga gcgcaacat 
ccagaacaa aatcaactc gctgaagga agaagtgaa 
gcgggaaat cgaagcttt gcggaagaa gcaagacga 
tgaagacga cgacgagga ggattacca aaggaagaa 
gcgccaagt gtacgacac tttgatgaa cgaagaaac 
cgaccaagt gaacccggc catgaatcc agacaatga 
gcttaaacc gcttgtcag gaaacggag acaagtcga 
tggggggaa aagactgga gcggtcttc cgacgctcg 
cccgcagga ggaggccga gcgggagaa aagtgaaga 
agggggtga attgctggg ggattttaa cgaaggagc 
ggtcgcaaa acgggaggt ttacagtcc ggatcagga 
atatgatga cgggttgta tgacaggaa taagcggga 
ttatgacag ttttttttc tgattatta ttacaataa 
tcgggcgaa gaggcaaaa tcgctttgt accgatgag 
gggaaatcg gtggaggag gtttaggag gaggattaa 
tgaagatca agatctttt tggagcgtt gccgaaaag 
ttacgatgg ggagttgtc aaggtataa gcgtgtcaa 
acggaaatg agttgtatt ttttaatgt attttttat 
taaatatat caactatta
      • >TC009431 RB
        gaaaatggc acaaatttc cgtttgttg ccgtttttt 
gatttttac atcaatgtg acatcagtc gcatttatt 
tttttgtac tcaactgca tattatgga aagaattta 
tgtcagcag cattggggc attcattct atatacact 
cagtactgt agcgcttgc aaaaccctt cgaggacga 
gaaaagttt cagtgatga caacaaatt acactgtgt 
ggactcaaa cgacgtcgg cctgaacaa tgagcggag 
caaccccaa ctcatttgc cgagttcta caaacctcg 
ctcaagatt cgaaggggt tgagaagtt cgcaaaccg 
gccgtttga ctttgacac gaaacgatc cgcagagga 
atcgcccgt caactcgct ggagaagcg gcaagttgg 
ggtggtgta caaagatga gatgccaat tggtatatt 
acaacctta tcaacgctc ttaccgctt tataccaat 
caaggaaca aagtccagc taatctcga tgctagtca 
ggctcagga tgttcctta cagtgtacc cagttttgc 
agatattga caaaaggga agtcaaaga gctggatga 
aacctaccc ttccatcct gacaaattt taaaattgt 
gaacaaact cgagaaaaa acgaaatta cgatgacaa 
aaacgttag ggatttgaa agcaacaat tcgccgctt 
gcgttccgc aaaccccca caacaacga gaaaattga 
agaagaatt gaagaatat cctggacga tatggggct 
cattgaaga tgaagaaag ccacggtgt gaagcgtga 
agccagcga tgattcgtt ggtcgagga agaattggt 
tgcagaaaa cgaacggaa caaaaggga tgataattg 
taataataa ccccaacag taagacaac ctacgacca 
caaccctgc cagtgataa taatcaaag gaacgaact 
caaaactga aaaccatcc aaatgtgta caagagaga 
gtcaagtga cgagtccga gcgccgaaa acggcaaaa 
aagcgaact tgtcgaaaa tctgaaaga ctcaggcga 
gttgatggt cagtgggaa caagtcacc cttggcaag 
ttccgaaat cacgaatga taagcgatc ctccgaaga 
ggaggaaga ttacgagtc gaggatcga gcgcaacat 
ccagaacaa aatcaactc gctgaagga agaagtgaa 
gcgggaaat cgaagcttt gcggaagaa gcaagacga 
tgaagacga cgacgagga ggattacca aaggaagaa 
gcgccaagt gtacgacac tttgatgaa cgaagaaac 
cgaccaagt gaacccggc catgaatcc agacaatga 
gcttaaacc gcttgtcag gaaacggag acaagtcga 
tggggggaa aagactgga gcggtcttc cgacgctcg 
cccgcagga ggaggccga gcgggagaa aagtgaaga 
agggggtga attgctggg ggattttaa cgaaggagc 
ggtcgcaaa acgggaggt ttacagtcc ggatcagga 
atatgatga cgggttgta tgacaggaa taagcggga 
ttatgacag ttttttttc tgattatta ttacaataa 
tcgggcgaa gaggcaaaa tcgctttgt accgatgag 
gggaaatcg gtggaggag gtttaggag gaggattaa 
tgaagatca aggtatggg taataggcc acaacagct 
cagtgatat gtctgattt agatctttt tggagcgtt 
gccgaaaag ttacgatgg ggagttgtc aaggtataa 
gcgtgtcaa acggaaatg agttgtatt ttttaatgt 
attttttat taaatatat caactatta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY