| General information | |||
| TC009430 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 1328860..1345184 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009430 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
For help on how to submit larger datasets or non experimental data please contact us.
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01550 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Fertility
ovary without detectable defects -
Number of animals showing the phenotype: 1
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
Days post injection
larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
Days post injection
- >TC009430 RA
MTVNKTTKT KKKSTASTS STTASTSKV TKSTKTHGE LSQASSSSS LQISDISHL PVDANIVTY TVTETLPTG GGEKITVYG ETGATSTEY RHFIAQDST AKQHSATIT QLSSKLNRS TSNISQESG STYTVTEPV EELKLTYNK NDSGWNGKF VYEQPVTKK GQGTVVEHS STSTSHEER SSIAKSSSS SYVIEIVDG KERIIDQKH HESASASSA VNDEHLAMK SGTNITPEV HYKQGAKKS STKYDTAIP ELTQPKTKT SESVREVHQ VGDSVSTTA HSISDSTKG HKSIHHDSK SKTRDSLGK STKGEEHKV TKSEVDFSH GSKTVRDES KRRESVDSN QTYTISDDK VGDLKKSSQ KHFTTYHDS KTERRESVD SNQTYTISD KSDKTSDLK KSSQEQFTT YYDSKTVRD EVGSKRRES LDSNQTYTI SGQTSDFRQ STRGEEHAK SSDFKSTRS GVEDFTSKS GDLRPKNGE LTTTTTTTY YDSKGNVIK TVQDVNTDE IAGKRRDSV DSNRTYTLS EKSFAGDST DEVSKRRTA QTTTDSRDF YGHGIDSSN TLVKTVYDD KAIQNTIGT KGRVIKDET IDSTDLVYS NERTYGKTG WNGKFTYET PRKSEAKRS PSPQKRGTS PAKKRTPSP STPKKGTSP DKTAPKRGT SPVKRKVTD TVQDFLSTE ISENINQCS TSDSKSTTD FQHMVPAPT DFKTFQTVS DSKTSFTSD SQSFVDVKN FVETSETVD IKNVDIRDT KVSNFVDKK ATVEHVTSK DVKDFRTSK DVTDRVNKV HIEENIVDI KTIDEVENI TNIERFSDN KVINIVKTI KETPKPATV KIPNKEQCI CELCTCGRH TCPHNPEDY NDHLHLEGP FLGQPKDEY NATRGERAP IIIPKDNLK PEGDFEKRQ PQKWQPGER ASVKIPEDH LKPEGEFEK RRPEEWKPG DRAPVKKPK DNLRPEGDF EKRKPEPWA PGERAPVRK PEDNLRPEG DFEKRKPEP WAPGDRAPV KKPQDNLKP EGDFEKRKP EPWAPGERA PVRKPEDNL RPEGDFEKR KPEPWAPGD RAPVKKPQD NLKPEGDFE KRKPEPWAP GERAPVRKP EDNLRPEGD FEKRRPEEW RPGDRAPVK KPEDNLKPE GDFEKRQPQ KWSPGERPK PKKPTDNLK PEGEFEKRT PEKWAPGER EPVKKPKDN LKPEGDFEI PEPEQWKPA ERSTPTKPK DNLQPEGDF ERPEPKKYQ PGERPVARK PQDNLKPEG EFTGTRTII EDYRVIRGE RADIKRHED NITIGGEFI DETTTKVDF NGELVPRPS PIRRNTWTK IEGEFITDT TNKSEFIDY STVVERTQI VKRRQDNLT VEGDMTFDT STTIDYTEK TPELEQRRR RTWTKDDVD KFYSTQNTE TTTTTQEVY KQFDNQDVR QTIIVHRDN LRPEGPFEG TPKSKDDFV PKYVERPKP KKPEDNLRP EGDFEKRTP EKFVPAERP KQKKPQDNL KPEGDFEKR TPEKFIPAE RPKQKRPED NLRPEGDFE RPTPTKPEG DFERPEKPG YRPAERPQP KKPEDNLRP EGDFERPEK PGYRPAERP KQKRPEDNL RPEGDFERP EKPGYRPAE RPKQKKPED NLRPEGDFE RPEKPGYRP AERPQPKKP EDNLRPEGD FERPEKPGY RPAERPKQK RPEDNLRPE GDFERPEKP GYRPAERPQ PKKPEDNLR PEGDFERPE KPGYRPAER PKQKKPEDN LRPEGDFER PEKPGYRPA ERPQQKRPE DNLRPEGDF ERPEKPQYR PAERPKQKK PEDNLRPEG DFERPTPTK VGPGERRTP IKHDDNLRP EGDFERPEK PGYRPAERP TPKKPEDNL RPEGEFERP EKPGYRPAE RPKQKRPED NLRPEGDFE RPTPTKVGL AERRTPIKH DDNLRPEGD FERPEKPEY RPAERPKQK RPEDNLRPE GDFERPEKP EFRPAERPK QKRPEDNLR PEGDFERPT PTKVGPAER RTPIKHDDN LRPEGDFER PEKPGYRPA ERPQPKRPE DNLRPEGEF ERPEKPGYR PAERPKPKK PEDNLRPEG DFERPTPTK VTLNVPKNL NTDRQKDRN KNVPKIILD LKVTLNGLK GPSLDQLNV RNKRNRKII FVQKEILKG RLQLKLSQL NVGHLLSTM IIYVLKEIS NVLRSLSTD RLKDLNLRN LKIIFILKA ILRDQLQPR LDQLKEEHQ LSMGIIFGL KEILNGLKN PNIDQLKDL NLKSLKIIF GLKAILKDR LQPRLVLLK EEHLSSTMI IFDLKETSK DPKNLNSDQ LNVRNLKNL KIIFALKVI LKDQPQPRL VLLKEGHQL SMVIIFGLK VTSNVLKNL NTDQLKDPN LRNLKIIFA QKATLKGQH LPRLDQLKK EHLLSTMII YVLKEISNV LKSLSTGRL KDPNLRNLK IIFVLKAIL KDRRQPRLD QLKGEHQLS MGIIFGLKE ILNGLKNPN IDQLKDLSL KSLKTIFVL KVISKGQHQ PKLVLLKGE HQLDTVTIS DLKETLKDP KNLNLNQLN VRNLKNPKI IFVLKANSK DQVQQKLDQ LKEEHQLNM VIISDLKET SNDLKSLNI DRLKDPNLK NPKIIFVQK EILKDRLQP KLVLLKEEH QLDTETISD LKETSNDPK NLNSDQLNV RNLKNPKIT FVLKVNSKD QVQQKPEKP KFKPAERPQ PKKPEDNLR PEGEFERPE LIEVGPCEL RKPIKHEDN LKPEGTLVV TRKDDYNVV KGERVDVIK REDNLRVEG EWDIRRRDD YSNTTVVDR VDVIKRQDN LRVEGEWDI RRRDDYSNT TVVDRVDVI KRQDNLRVE GEFTDVRTR DDYKVVKGE RYDTVIHQD NLKTEGEFR SSRTSDEYS VVKGERAEI ITRQDNLRM EGEFNEYRK RDEFNTRIV EKAEVIRHQ DNLKLEGTF ERPSHVHLG PGERRKPIK HEDNLHPEG DFERPDRPG FKPAERPSP IKPQDNLKP EGKFELPTS PGFQPGERR TPIRHQDNL HPEGDFERP QRPQYQPIE RPTPKKPKD NLKPEGTFE RPTASPVGP GERRSPIRH HDNLRPEGA FERPDKPGY KPGERPTPK KPQDNLRPE GEFEKRTPA KPQPGERRT PIRHQDNLH PEGAFERPE KPKYSPGER LPMKKPQDN LRPEGEFET PQKPKYGPG ERAPIVKHP DNLKPEGEF IGTPKSKDD FKPTRGERA PVKKPQDNL KITGEFQDM TMQKSQFVV TRGERAEIR KHEDSLTIG NGKTEYITT SKETFDKTT PTKVVTSPA TPGSINRRR HIESHITLG SDTAVMQTT NQSNYNTYT KKIDRQSTT GRNEVANKT TNVATDSKT LADGTTVTT TRTTTKTTG QQLAKQTQQ VSAEVSTHH RKNVITSEE INNQILHRK GLHTSTEAL HATSSAALD MRKSICNLH DQGHVSQNS ERRSLSSIH RSTQESNAS RRSHFATSY ETVERPQKI IRKDNLSVG GHFYGQSEA RSYGEFTKQ QNVQKIERV TRRSNVSNI SLGDSSSTI ITSYKKEYL PRNVGPCPA VLIDLPKSP LKHTRDTKS HKYYAPVSS K
- >TC009430 RA
atgacggtg aataagaca accaagacg aaaaaaaag agcacagct tcgacctcc tcgacaacg gcaagtacc tccaaagtc accaaatcg acaaaaaca cacggcgag ctatcgcaa gcctcctct tcgtccagt ttgcaaatt tccgatatt tcgcattta ccggttgac gctaacatc gtcacatac acggtaacg gagacttta ccgaccgga ggcggcgag aaaatcaca gtgtatggc gaaactggc gccacaagc actgaatac cgccacttt atcgcccaa gattcgaca gcgaaacaa cactccgcc actatcaca caactcagc tcgaaacta aaccgctcc acttcgaac atatcgcaa gaatccggc tccacgtac actgtaacc gaaccggtg gaagaactc aaactaact tacaataaa aacgattct ggatggaac gggaagttc gtttacgaa caacctgtc acaaaaaag ggacaaggg acagttgtc gaacactcc tccacgtcc acgtcacac gaggaacgt tcttcaatt gccaaaagt tcgtccagt agttacgtt atcgaaata gtcgatggt aaagaacgc ataattgac cagaaacac catgagtcc gccagtgcg tcttctgca gtcaatgac gaacatttg gccatgaaa agtggcact aacatcaca ccagaagtg cactacaag caaggggcg aagaaaagt tcgacaaag tatgatact gctattcct gaactaaca caacccaaa actaaaact tccgaatct gtgagagaa gtgcaccag gtcggagac tccgtttcg acgacagct cactccatt agcgactcg acaaaagga cacaaatcg atccatcac gattccaaa agcaaaaca agagattcc ttaggaaag tcaacaaag ggggaagaa cataaagtg acaaagagc gaagttgat ttctctcac ggttccaaa accgtgaga gatgagagc aagagaaga gaatcagtt gattcgaac caaacctac accattagt gatgacaaa gttggcgac ttgaaaaaa tcgtcccaa aaacatttc acaacttat cacgattcc aaaactgag agaagagag tcagttgat tcgaaccaa acctacacc attagtgac aaaagcgat aaaacaagt gacttgaaa aagtcgtca caagaacag ttcacaact tattacgac tccaaaact gtaagagat gaagttggt agtaagagg agagaatca cttgattcg aaccaaacc tacaccatt agtggccaa actagcgac ttccgacag tcaacaaga ggcgaagag cacgccaag tctagtgat ttcaagtct acaagaagt ggagtggaa gatttcacc tccaaatct ggcgatctt cgcccaaaa aacggcgaa ctgaccacc accaccact accacctac tacgattct aaaggcaat gtgataaag acagtccag gacgtgaac actgatgaa atcgccggt aagaggcgt gattcagtc gactcaaac cgcacctac accctgagc gagaaaagt ttcgccggc gattcaacc gacgaagtc tcaaaacgg cgaactgct cagaccacc accgattcg cgcgacttt tacggccat gggatcgat tcgtcaaac actctcgtg aagacagtt tacgacgat aaagccatc cagaacaca atcggtacc aaaggcaga gtaatcaag gacgaaacg atcgattcg accgacttg gtctattca aacgaacgc acttatgga aaaacaggc tggaatggg aagtttacc tacgaaacg cccagaaag agtgaagct aaacgctcg ccctcgccc caaaaacgg ggcacttcg ccggcgaaa aagcgaaca ccgtcgcct tcgacacct aaaaaggga acttcgccg gataaaacg gcaccgaaa aggggaact tcgccggtg aagcgaaaa gtgactgat accgtccag gatttttta tcgactgaa atcagtgag aacatcaac cagtgcagt acaagtgat tcgaaaagt accacggat ttccaacac atggtgcca gctcctact gatttcaag accttccaa acggtctca gactcgaaa actagcttc acttcggac agccaaagt tttgtcgat gtgaaaaat ttcgttgag acaagtgag actgtcgat atcaaaaat gttgatatc agggacacg aaagtgagc aactttgtg gacaagaaa gcgaccgtc gaacatgtg accagtaag gatgttaag gattttcgc acgtccaag gatgtcaca gatagggtt aataaagtg cacattgag gagaacatt gtcgatatt aagaccatt gacgaggtc gagaatatc acaaatatt gaacggttc agcgataat aaagttata aatattgtg aagaccatc aaagaaaca ccgaagcct gcaactgtt aagattccc aataaggag caatgtatc tgcgagttg tgcacgtgt ggacgtcac acgtgtcct cacaacccc gaagactac aacgatcac ttacacctc gaaggcccc tttctaggc caacctaaa gacgaatac aatgcaacc agaggcgaa agagcccca attatcata cctaaagac aacctaaaa ccagaaggg gattttgaa aaacgccag ccacaaaaa tggcaacct ggcgaaaga gcctctgta aaaataccg gaagaccac ctaaaacct gaaggagaa tttgaaaag agacgacct gaagaatgg aaacctggc gatcgtgcg cctgtgaaa aaacctaaa gataatcta cgacctgaa ggagatttc gaaaaacgc aaacctgaa ccctgggca cctggagaa agggctcca gtgcgcaaa cctgaagat aatctaaga ccagaaggc gattttgaa aaacgcaaa cctgaacct tgggcacct ggtgatcgg gcgcccgtt aagaaaccc caagacaac ctaaaaccg gaaggtgat tttgaaaaa cggaagcct gaaccttgg gcacctgga gaaagggct ccagtgcgc aaacctgaa gacaaccta agaccggag ggtgatttt gaaaaacgg aagcctgaa ccttgggca cctggcgac cgggcgccc gttaagaaa ccccaagac aacctaaaa ccggaaggt gattttgaa aaacggaag cctgaacct tgggcacct ggagaaagg gcgccagtg cgcaaacct gaagacaac ctaagacca gaaggcgat tttgaaaaa cgccgcccc gaggaatgg agacctggc gatcgggcg cccgttaaa aaacctgaa gacaaccta aaacctgaa ggggatttc gaaaaacgc caacctcag aaatggtca cctggtgaa cgaccaaaa cctaaaaaa cctaccgat aatttgaaa cctgaaggc gagtttgag aaacgaacg cctgaaaaa tgggctcct ggggagcga gaaccggtg aagaaacct aaagataat ttgaaacct gagggcgat tttgaaata cctgagcct gagcagtgg aaacctgcc gaaaggtct acgcctacc aaacctaaa gataatctg caacctgag ggtgatttt gaacgacct gagcctaag aaatatcaa ccgggtgaa aggccagtt gcaaggaag ccgcaggat aatttgaaa cctgaaggt gaatttact gggactagg accattatt gaggattat cgtgtgatt cggggagaa cgcgccgat atcaagcgt catgaagac aacatcact ataggaggt gaatttata gacgaaact accactaaa gttgatttt aatggcgag ttagtacca cgtcctagc ccaatccga cgcaacact tggacgaaa atcgagggc gaatttatc acagatacg accaataaa tccgaattt attgattac agtactgtt gttgaaaga acacaaatt gtgaaaaga cggcaagat aatctcact gttgaggga gacatgact tttgatact tcaaccacg atcgattat acagaaaaa acacccgaa ctcgaacaa aggcgtcgc agaacttgg acgaaagat gacgtcgat aaattctat tcaacgcaa aacactgaa acaacaaca acaacgcaa gaagtttac aaacagttt gataaccaa gacgttcgc caaacaatt attgtacac agagataac ctcaggccg gagggacct tttgaagga acaccaaaa tcaaaggat gacttcgtc cctaaatat gttgaacgt cctaaaccg aaaaaacct gaagataat ttaagacca gagggtgat tttgaaaaa cgcacacct gagaaattt gttcctgcg gaacgacct aagcaaaag aaaccgcaa gataatttg aaacctgaa ggcgatttt gagaaacgc actcccgag aaatttatt ccagccgaa aggcctaaa cagaagaga cctgaagat aatcttcgc ccagaaggt gattttgaa agacctact ccgaccaag cctgagggt gattttgaa agacccgaa aagcctgga tacaggcct gcagaacgt ccacaacct aaaaaacct gaagataat ttaaggcct gagggagat tttgaacgt ccagagaaa cctggatat cgtccagct gagcgtcca aaacagaaa cgtcctgag gataatttg aggcctgag ggagatttt gaaagacct gaaaaacct ggatatcgc ccagctgaa cgaccaaaa cagaaaaaa cctgaggat aatttgagg cctgaaggt gactttgaa agacctgaa aagcctgga tataggcct gcagaacga ccacaaccg aaaaaacct gaagataac ttgcggcct gaaggagat tttgaacgt ccagagaaa cctggatat cgtccagct gaacgtcca aaacaaaag agacctgag gataatttg aggcctgaa ggtgacttt gaaagacct gaaaaacct ggatatagg cctgcagaa cgtccacag cctaaaaaa cctgaagat aatttgcgg cctgaagga gattttgaa cgtccagag aaacctgga tatcgtcca gctgagcgt cctaaacag aagaaacct gaggataat ttgaggcct gagggagat tttgaaagg cctgaaaaa cctggatat cgcccagct gaacgtcct caacaaaaa cgccctgaa gataatcta agaccagaa ggtgatttt gaaaggccg gaaaaaccg caatatcgt ccagctgag cgtccaaaa cagaagaaa cctgaagat aatctcaga cctgagggc gactttgaa agaccaacc ccaaccaag gttggtcca ggtgaaaga agaacacct attaagcat gacgataac cttagacct gaaggtgat tttgaacgt ccagaaaag cctggatat cgtcctgct gaacgtcca actcctaaa aaacctgaa gataatctt cggccagaa ggtgaattt gagaggccg gagaaacct ggatatcgt ccagcagaa cgtccgaaa caaaagcgt ccggaagat aatttaagg cctgaagga gattttgaa agaccaaca cctacgaaa gttgggctg gcagaacgc agaactcca attaaacat gatgataat ttgagacct gaaggtgac tttgaacgt cctgaaaaa cctgaatac cgaccggca gaaagaccg aaacaaaaa cgtcctgaa gacaatctt aggcctgaa ggtgatttt gaacggccg gaaaaaccc gaatttagg ccagcagaa cgtcctaaa cagaaaaga cctgaagat aatcttcgt ccagaagga gattttgaa agaccgact ccaactaaa gttgggcca gctgaacgt aggacacct atcaagcat gatgataat ctacgtcct gaaggagat ttcgaacgt cctgaaaaa cctggatat aggcctgca gaacggcca caacctaaa aggcctgaa gataattta aggcctgaa ggtgaattt gagaggccg gagaaacct ggatatcgt ccagctgaa cgtccgaaa cctaagaaa cctgaagat aatctccga cctgaagga gattttgaa agacctact cctacgaaa gtgactttg aacgtcccg aaaaacctg aataccgac cggcagaaa gaccgaaac aaaaacgtc ccgaagata atcttagac ctgaaggtg actttgaac ggcctgaaa ggcccgagt ttagaccag ctgaacgtc cgaaacaaa agaaaccgg aagataatc ttcgtccag aaggagatt ttgaaaggc cgactccaa ctaaagttg agccagctg aacgtagga cacctatta agcacgatg ataatctac gtcctgaag gagatttcg aacgtcctg agaagcctc agtacagac cggctgaaa gacctcaac ctaagaaac ctgaagata atcttcatc ctgaaggcg attttgaga gaccaactc caaccaagg ttggaccag ctgaaagaa gaacaccaa ttaagcatg gggataatc ttcggcctg aaggagatt ttgaacggc ctgaaaaac cccaatata gaccagctg aaagacctc aacctaaaa agcctgaag ataatcttc ggcctgaag gcgattttg aaagaccga ctccaacca aggttggtc ctgctgaaa gaagaacac ctatcaagc acgatgata atcttcgac ctgaaggag acttcgaaa gacccgaaa aacctgaat tcagaccag ctgaacgtc cgaaaccta aaaaacctg aagataatc ttcgccctg aaggtgatt ttgaaagac caaccccaa ccaaggttg gtcctgctg aaagaagga caccaatta agcatggtg ataatcttc ggcctgaag gtgacttcg aacgtcctg aaaaacctc aatacagac cagctgaaa gaccccaac ctaagaaac ctgaagata atcttcgcc cagaaggcg actttgaaa ggccaacac ctaccaagg ttggaccag ctgaaaaaa gaacaccta ttaagcacg atgataatt tacgtcctg aaggagatt tcgaacgtc ctgaaaagc ctcagtaca ggccggctg aaagacccc aacctaaga aacctgaag ataatcttc gtcctgaag gcgattttg aaagaccga cgccaacca aggttggac cagctgaaa ggagaacac caattaagc atggggata atcttcggc ctgaaggag attttgaac ggcctgaaa aaccccaat atagaccag ctgaaagac ctcagccta aaaagcctg aagacaatc ttcgtcctg aaggtgatt tcgaaaggc caacaccaa ccaaagttg gtcctgctg aaaggagaa caccaatta gacacggtg acaatctcc gacctgaag gagactttg aaagacccg aaaaacctg aatttaaac cagctgaac gtccgaaac ctaaaaaac ccgaagata atcttcgtc ctgaaggcg aattcgaaa gaccaagtc caacaaaag ttggaccag ctgaaagaa gaacaccaa ttaaacatg gtgataatc tccgacctg aaggagact tcgaacgac ctgaaaagc ctcaatata gaccggctg aaagacccc aacctaaaa aacccgaag ataatcttc gtccagaag gagattttg aaagaccga ctccaacca aagttggtc ctgctgaaa gaagaacac caattagac acggagaca atctccgac ctgaaggag acttcgaac gacccgaaa aacctgaat tcagaccag ctgaacgtc cgaaaccta aaaaacccg aagataacc ttcgtcctg aaggtgaat tcgaaagac caagtccaa caaaaaccc gaaaaaccc aaattcaaa ccagctgaa cgtccccaa cctaaaaaa cctgaagac aatcttcgt cctgaaggt gaattcgaa cggcctgag ctaatagaa gtgggcccg tgtgaattg cgcaaacct atcaaacat gaagataat ctcaaacct gaaggaaca cttgttgtt accagaaag gacgattac aacgttgtg aaaggcgaa cgagttgat gtgatcaaa cgtgaagat aatttacgt gtcgaagga gaatgggat attcggcga agagacgat tacagtaac actacagtc gtagatcgg gtcgatgtt atcaaacgc caagacaat ctacgtgtt gaaggagaa tgggatatt agacgtaga gatgactac agtaataca acggtcgta gatcgcgtc gatgtcatc aaacgccaa gacaattta cgcgtcgag ggcgaattc accgatgta cgaacacga gatgactac aaagtcgtc aagggcgaa agatacgat actgtcatt caccaagac aatctaaaa acagaagga gaattccga agtagtcgc actagcgac gaatactcc gttgtcaaa ggcgaacgc gcagaaatc atcaccaga caagacaat ctccgaatg gaaggcgaa ttcaacgaa taccgaaaa cgcgacgaa ttcaacact cgaattgtc gaaaaagct gaagtgatc cgccaccaa gacaacctg aaactggaa ggcaccttc gaacgcccg tcccacgtc cacctagga cctggcgaa cgtcgcaaa ccaatcaaa cacgaagac aacctccac cctgaaggc gacttcgaa cgcccagac cgccccggc tttaaacca gccgaaagg ccgtcacca atcaaaccc caagacaat ctcaaacct gaaggaaaa ttcgaacta ccaacttcc cccggtttc caacccggc gaacgtcgg actccgatt cgccaccag gataacttg cacccagaa ggcgacttc gaacgtccc caaagacca caataccaa ccaatcgaa cgcccaaca cccaaaaaa cccaaagac aaccttaaa cccgaagga acattcgaa cgtccaacg gcaagtccc gtgggaccc ggcgaacgg cgaagccca atccgccac catgacaat ttaagacct gaaggtgcg ttcgaacgc ccagacaag cccggctat aaaccaggc gaacgccca actccgaaa aaaccccag gataaccta cgcccagaa ggggaattc gaaaaacga accccggcg aaaccacaa cctggcgaa cggcgaact ccgatccgc caccaggat aatctgcac cctgagggt gctttcgag cgacccgag aagcctaaa tactcgcct ggtgaacgc ctaccaatg aagaaaccc caagataat ttgagaccc gaaggtgag tttgaaacc ccccagaaa cctaaatac gggcctggc gaacgtgcc cccatcgtc aaacaccca gacaacctt aaacctgaa ggcgaattc atcggaaca cccaaaagt aaagacgat tttaaacca actcggggc gaacgagct ccggttaag aaaccacaa gacaatttg aaaatcaca ggagagttc caagacatg acaatgcag aaatcacag tttgtcgta acccgaggt gagagagca gaaatacgc aaacatgaa gattcccta actataggg aacggcaaa actgaatat atcaccaca tcgaaggaa acgtttgat aaaacaacg cccacgaaa gtggtcaca agtccagca actcctgga tccatcaac aggagacgc cacattgag tcacacatt actctaggg agtgacaca gcagtgatg caaacaacg aaccaaagc aactacaac acttacacc aagaaaatt gatcgccag tcaacaaca ggccgtaac gaagttgca aataaaaca acgaacgtt gcaacggat agtaagact ttggctgac ggtacaact gtcacaaca actcgcaca acaaccaaa acaacagga caacaactg gcgaaacaa acccagcaa gtctccgcc gaagtgtca acccaccac cgcaaaaac gtcataact tcggaggaa atcaacaac cagatcctg cacaggaaa gggctgcac acctcgacc gaagccctg catgccacg agttcggca gccctagac atgcgaaag tcaatctgc aatttgcac gaccaaggc cacgtgagc cagaacagc gaacggcga agtctcagc tcgatccat cgctcgacg caggagagc aacgccagc cggcgctcc cactttgcc accagttac gagacggtg gaaaggccg cagaagatc atccgcaag gataattta tcagtcggg gggcatttc tacggccag tcggaggcc aggtcgtat ggcgagttc accaagcag cagaatgtg caaaaaatt gaacgggtg actcgcagg tccaacgtt tcgaatata agtttaggg gacagttct tcgacgatc atcacgtcg tataagaag gagtatttg ccgcggaat gtgggcccg tgtccggcg gttttgatc gatttgccc aaaagtccc ctgaagcat acgagggac accaagtcg cataaatat tacgcgccg gtttcgagt aaatga
- >TC009430 RA
tcactttca aaaagagcg cgaccgcga acgcacgca ccgccaata tttgtgtta ttaactttg tgtgaaaat gacggtgaa taagacaac caagacgaa aaaaaagag cacagcttc gacctcctc gacaacggc aagtacctc caaagtcac caaatcgac aaaaacaca cggcgagct atcgcaagc ctcctcttc gtccagttt gcaaatttc cgatatttc gcatttacc ggttgacgc taacatcgt cacatacac ggtaacgga gactttacc gaccggagg cggcgagaa aatcacagt gtatggcga aactggcgc cacaagcac tgaataccg ccactttat cgcccaaga ttcgacagc gaaacaaca ctccgccac tatcacaca actcagctc gaaactaaa ccgctccac ttcgaacat atcgcaaga atccggctc cacgtacac tgtaaccga accggtgga agaactcaa actaactta caataaaaa cgattctgg atggaacgg gaagttcgt ttacgaaca acctgtcac aaaaaaggg acaagggac agttgtcga acactcctc cacgtccac gtcacacga ggaacgttc ttcaattgc caaaagttc gtccagtag ttacgttat cgaaatagt cgatggtaa agaacgcat aattgacca gaaacacca tgagtccgc cagtgcgtc ttctgcagt caatgacga acatttggc catgaaaag tggcactaa catcacacc agaagtgca ctacaagca aggggcgaa gaaaagttc gacaaagta tgatactgc tattcctga actaacaca acccaaaac taaaacttc cgaatctgt gagagaagt gcaccaggt cggagactc cgtttcgac gacagctca ctccattag cgactcgac aaaaggaca caaatcgat ccatcacga ttccaaaag caaaacaag agattcctt aggaaagtc aacaaaggg ggaagaaca taaagtgac aaagagcga agttgattt ctctcacgg ttccaaaac cgtgagaga tgagagcaa gagaagaga atcagttga ttcgaacca aacctacac cattagtga tgacaaagt tggcgactt gaaaaaatc gtcccaaaa acatttcac aacttatca cgattccaa aactgagag aagagagtc agttgattc gaaccaaac ctacaccat tagtgacaa aagcgataa aacaagtga cttgaaaaa gtcgtcaca agaacagtt cacaactta ttacgactc caaaactgt aagagatga agttggtag taagaggag agaatcact tgattcgaa ccaaaccta caccattag tggccaaac tagcgactt ccgacagtc aacaagagg cgaagagca cgccaagtc tagtgattt caagtctac aagaagtgg agtggaaga tttcacctc caaatctgg cgatcttcg cccaaaaaa cggcgaact gaccaccac caccactac cacctacta cgattctaa aggcaatgt gataaagac agtccagga cgtgaacac tgatgaaat cgccggtaa gaggcgtga ttcagtcga ctcaaaccg cacctacac cctgagcga gaaaagttt cgccggcga ttcaaccga cgaagtctc aaaacggcg aactgctca gaccaccac cgattcgcg cgactttta cggccatgg gatcgattc gtcaaacac tctcgtgaa gacagttta cgacgataa agccatcca gaacacaat cggtaccaa aggcagagt aatcaagga cgaaacgat cgattcgac cgacttggt ctattcaaa cgaacgcac ttatggaaa aacaggctg gaatgggaa gtttaccta cgaaacgcc cagaaagag tgaagctaa acgctcgcc ctcgcccca aaaacgggg cacttcgcc ggcgaaaaa gcgaacacc gtcgccttc gacacctaa aaagggaac ttcgccgga taaaacggc accgaaaag gggaacttc gccggtgaa gcgaaaagt gactgatac cgtccagga ttttttatc gactgaaat cagtgagaa catcaacca gtgcagtac aagtgattc gaaaagtac cacggattt ccaacacat ggtgccagc tcctactga tttcaagac cttccaaac ggtctcaga ctcgaaaac tagcttcac ttcggacag ccaaagttt tgtcgatgt gaaaaattt cgttgagac aagtgagac tgtcgatat caaaaatgt tgatatcag ggacacgaa agtgagcaa ctttgtgga caagaaagc gaccgtcga acatgtgac cagtaagga tgttaagga ttttcgcac gtccaagga tgtcacaga tagggttaa taaagtgca cattgagga gaacattgt cgatattaa gaccattga cgaggtcga gaatatcac aaatattga acggttcag cgataataa agttataaa tattgtgaa gaccatcaa agaaacacc gaagcctgc aactgttaa gattcccaa taaggagca atgtatctg cgagttgtg cacgtgtgg acgtcacac gtgtcctca caaccccga agactacaa cgatcactt acacctcga aggcccctt tctaggcca acctaaaga cgaatacaa tgcaaccag aggcgaaag agccccaat tatcatacc taaagacaa cctaaaacc agaagggga ttttgaaaa acgccagcc acaaaaatg gcaacctgg cgaaagagc ctctgtaaa aataccgga agaccacct aaaacctga aggagaatt tgaaaagag acgacctga agaatggaa acctggcga tcgtgcgcc tgtgaaaaa acctaaaga taatctacg acctgaagg agatttcga aaaacgcaa acctgaacc ctgggcacc tggagaaag ggctccagt gcgcaaacc tgaagataa tctaagacc agaaggcga ttttgaaaa acgcaaacc tgaaccttg ggcacctgg tgatcgggc gcccgttaa gaaacccca agacaacct aaaaccgga aggtgattt tgaaaaacg gaagcctga accttgggc acctggaga aagggctcc agtgcgcaa acctgaaga caacctaag accggaggg tgattttga aaaacggaa gcctgaacc ttgggcacc tggcgaccg ggcgcccgt taagaaacc ccaagacaa cctaaaacc ggaaggtga ttttgaaaa acggaagcc tgaaccttg ggcacctgg agaaagggc gccagtgcg caaacctga agacaacct aagaccaga aggcgattt tgaaaaacg ccgccccga ggaatggag acctggcga tcgggcgcc cgttaaaaa acctgaaga caacctaaa acctgaagg ggatttcga aaaacgcca acctcagaa atggtcacc tggtgaacg accaaaacc taaaaaacc taccgataa tttgaaacc tgaaggcga gtttgagaa acgaacgcc tgaaaaatg ggctcctgg ggagcgaga accggtgaa gaaacctaa agataattt gaaacctga gggcgattt tgaaatacc tgagcctga gcagtggaa acctgccga aaggtctac gcctaccaa acctaaaga taatctgca acctgaggg tgattttga acgacctga gcctaagaa atatcaacc gggtgaaag gccagttgc aaggaagcc gcaggataa tttgaaacc tgaaggtga atttactgg gactaggac cattattga ggattatcg tgtgattcg gggagaacg cgccgatat caagcgtca tgaagacaa catcactat aggaggtga atttataga cgaaactac cactaaagt tgattttaa tggcgagtt agtaccacg tcctagccc aatccgacg caacacttg gacgaaaat cgagggcga atttatcac agatacgac caataaatc cgaatttat tgattacag tactgttgt tgaaagaac acaaattgt gaaaagacg gcaagataa tctcactgt tgagggaga catgacttt tgatacttc aaccacgat cgattatac agaaaaaac acccgaact cgaacaaag gcgtcgcag aacttggac gaaagatga cgtcgataa attctattc aacgcaaaa cactgaaac aacaacaac aacgcaaga agtttacaa acagtttga taaccaaga cgttcgcca aacaattat tgtacacag agataacct caggccgga gggaccttt tgaaggaac accaaaatc aaaggatga cttcgtccc taaatatgt tgaacgtcc taaaccgaa aaaacctga agataattt aagaccaga gggtgattt tgaaaaacg cacacctga gaaatttgt tcctgcgga acgacctaa gcaaaagaa accgcaaga taatttgaa acctgaagg cgattttga gaaacgcac tcccgagaa atttattcc agccgaaag gcctaaaca gaagagacc tgaagataa tcttcgccc agaaggtga ttttgaaag acctactcc gaccaagcc tgagggtga ttttgaaag acccgaaaa gcctggata caggcctgc agaacgtcc acaacctaa aaaacctga agataattt aaggcctga gggagattt tgaacgtcc agagaaacc tggatatcg tccagctga gcgtccaaa acagaaacg tcctgagga taatttgag gcctgaggg agattttga aagacctga aaaacctgg atatcgccc agctgaacg accaaaaca gaaaaaacc tgaggataa tttgaggcc tgaaggtga ctttgaaag acctgaaaa gcctggata taggcctgc agaacgacc acaaccgaa aaaacctga agataactt gcggcctga aggagattt tgaacgtcc agagaaacc tggatatcg tccagctga acgtccaaa acaaaagag acctgagga taatttgag gcctgaagg tgactttga aagacctga aaaacctgg atataggcc tgcagaacg tccacagcc taaaaaacc tgaagataa tttgcggcc tgaaggaga ttttgaacg tccagagaa acctggata tcgtccagc tgagcgtcc taaacagaa gaaacctga ggataattt gaggcctga gggagattt tgaaaggcc tgaaaaacc tggatatcg cccagctga acgtcctca acaaaaacg ccctgaaga taatctaag accagaagg tgattttga aaggccgga aaaaccgca atatcgtcc agctgagcg tccaaaaca gaagaaacc tgaagataa tctcagacc tgagggcga ctttgaaag accaacccc aaccaaggt tggtccagg tgaaagaag aacacctat taagcatga cgataacct tagacctga aggtgattt tgaacgtcc agaaaagcc tggatatcg tcctgctga acgtccaac tcctaaaaa acctgaaga taatcttcg gccagaagg tgaatttga gaggccgga gaaacctgg atatcgtcc agcagaacg tccgaaaca aaagcgtcc ggaagataa tttaaggcc tgaaggaga ttttgaaag accaacacc tacgaaagt tgggctggc agaacgcag aactccaat taaacatga tgataattt gagacctga aggtgactt tgaacgtcc tgaaaaacc tgaataccg accggcaga aagaccgaa acaaaaacg tcctgaaga caatcttag gcctgaagg tgattttga acggccgga aaaacccga atttaggcc agcagaacg tcctaaaca gaaaagacc tgaagataa tcttcgtcc agaaggaga ttttgaaag accgactcc aactaaagt tgggccagc tgaacgtag gacacctat caagcatga tgataatct acgtcctga aggagattt cgaacgtcc tgaaaaacc tggatatag gcctgcaga acggccaca acctaaaag gcctgaaga taatttaag gcctgaagg tgaatttga gaggccgga gaaacctgg atatcgtcc agctgaacg tccgaaacc taagaaacc tgaagataa tctccgacc tgaaggaga ttttgaaag acctactcc tacgaaagt gactttgaa cgtcccgaa aaacctgaa taccgaccg gcagaaaga ccgaaacaa aaacgtccc gaagataat cttagacct gaaggtgac tttgaacgg cctgaaagg cccgagttt agaccagct gaacgtccg aaacaaaag aaaccggaa gataatctt cgtccagaa ggagatttt gaaaggccg actccaact aaagttgag ccagctgaa cgtaggaca cctattaag cacgatgat aatctacgt cctgaagga gatttcgaa cgtcctgag aagcctcag tacagaccg gctgaaaga cctcaacct aagaaacct gaagataat cttcatcct gaaggcgat tttgagaga ccaactcca accaaggtt ggaccagct gaaagaaga acaccaatt aagcatggg gataatctt cggcctgaa ggagatttt gaacggcct gaaaaaccc caatataga ccagctgaa agacctcaa cctaaaaag cctgaagat aatcttcgg cctgaaggc gattttgaa agaccgact ccaaccaag gttggtcct gctgaaaga agaacacct atcaagcac gatgataat cttcgacct gaaggagac ttcgaaaga cccgaaaaa cctgaattc agaccagct gaacgtccg aaacctaaa aaacctgaa gataatctt cgccctgaa ggtgatttt gaaagacca accccaacc aaggttggt cctgctgaa agaaggaca ccaattaag catggtgat aatcttcgg cctgaaggt gacttcgaa cgtcctgaa aaacctcaa tacagacca gctgaaaga ccccaacct aagaaacct gaagataat cttcgccca gaaggcgac tttgaaagg ccaacacct accaaggtt ggaccagct gaaaaaaga acacctatt aagcacgat gataattta cgtcctgaa ggagatttc gaacgtcct gaaaagcct cagtacagg ccggctgaa agaccccaa cctaagaaa cctgaagat aatcttcgt cctgaaggc gattttgaa agaccgacg ccaaccaag gttggacca gctgaaagg agaacacca attaagcat ggggataat cttcggcct gaaggagat tttgaacgg cctgaaaaa ccccaatat agaccagct gaaagacct cagcctaaa aagcctgaa gacaatctt cgtcctgaa ggtgatttc gaaaggcca acaccaacc aaagttggt cctgctgaa aggagaaca ccaattaga cacggtgac aatctccga cctgaagga gactttgaa agacccgaa aaacctgaa tttaaacca gctgaacgt ccgaaacct aaaaaaccc gaagataat cttcgtcct gaaggcgaa ttcgaaaga ccaagtcca acaaaagtt ggaccagct gaaagaaga acaccaatt aaacatggt gataatctc cgacctgaa ggagacttc gaacgacct gaaaagcct caatataga ccggctgaa agaccccaa cctaaaaaa cccgaagat aatcttcgt ccagaagga gattttgaa agaccgact ccaaccaaa gttggtcct gctgaaaga agaacacca attagacac ggagacaat ctccgacct gaaggagac ttcgaacga cccgaaaaa cctgaattc agaccagct gaacgtccg aaacctaaa aaacccgaa gataacctt cgtcctgaa ggtgaattc gaaagacca agtccaaca aaaacccga aaaacccaa attcaaacc agctgaacg tccccaacc taaaaaacc tgaagacaa tcttcgtcc tgaaggtga attcgaacg gcctgagct aatagaagt gggcccgtg tgaattgcg caaacctat caaacatga agataatct caaacctga aggaacact tgttgttac cagaaagga cgattacaa cgttgtgaa aggcgaacg agttgatgt gatcaaacg tgaagataa tttacgtgt cgaaggaga atgggatat tcggcgaag agacgatta cagtaacac tacagtcgt agatcgggt cgatgttat caaacgcca agacaatct acgtgttga aggagaatg ggatattag acgtagaga tgactacag taatacaac ggtcgtaga tcgcgtcga tgtcatcaa acgccaaga caatttacg cgtcgaggg cgaattcac cgatgtacg aacacgaga tgactacaa agtcgtcaa gggcgaaag atacgatac tgtcattca ccaagacaa tctaaaaac agaaggaga attccgaag tagtcgcac tagcgacga atactccgt tgtcaaagg cgaacgcgc agaaatcat caccagaca agacaatct ccgaatgga aggcgaatt caacgaata ccgaaaacg cgacgaatt caacactcg aattgtcga aaaagctga agtgatccg ccaccaaga caacctgaa actggaagg caccttcga acgcccgtc ccacgtcca cctaggacc tggcgaacg tcgcaaacc aatcaaaca cgaagacaa cctccaccc tgaaggcga cttcgaacg cccagaccg ccccggctt taaaccagc cgaaaggcc gtcaccaat caaacccca agacaatct caaacctga aggaaaatt cgaactacc aacttcccc cggtttcca acccggcga acgtcggac tccgattcg ccaccagga taacttgca cccagaagg cgacttcga acgtcccca aagaccaca ataccaacc aatcgaacg cccaacacc caaaaaacc caaagacaa ccttaaacc cgaaggaac attcgaacg tccaacggc aagtcccgt gggacccgg cgaacggcg aagcccaat ccgccacca tgacaattt aagacctga aggtgcgtt cgaacgccc agacaagcc cggctataa accaggcga acgcccaac tccgaaaaa accccagga taacctacg cccagaagg ggaattcga aaaacgaac cccggcgaa accacaacc tggcgaacg gcgaactcc gatccgcca ccaggataa tctgcaccc tgagggtgc tttcgagcg acccgagaa gcctaaata ctcgcctgg tgaacgcct accaatgaa gaaacccca agataattt gagacccga aggtgagtt tgaaacccc ccagaaacc taaatacgg gcctggcga acgtgcccc catcgtcaa acacccaga caaccttaa acctgaagg cgaattcat cggaacacc caaaagtaa agacgattt taaaccaac tcggggcga acgagctcc ggttaagaa accacaaga caatttgaa aatcacagg agagttcca agacatgac aatgcagaa atcacagtt tgtcgtaac ccgaggtga gagagcaga aatacgcaa acatgaaga ttccctaac tatagggaa cggcaaaac tgaatatat caccacatc gaaggaaac gtttgataa aacaacgcc cacgaaagt ggtcacaag tccagcaac tcctggatc catcaacag gagacgcca cattgagtc acacattac tctagggag tgacacagc agtgatgca aacaacgaa ccaaagcaa ctacaacac ttacaccaa gaaaattga tcgccagtc aacaacagg ccgtaacga agttgcaaa taaaacaac gaacgttgc aacggatag taagacttt ggctgacgg tacaactgt cacaacaac tcgcacaac aaccaaaac aacaggaca acaactggc gaaacaaac ccagcaagt ctccgccga agtgtcaac ccaccaccg caaaaacgt cataacttc ggaggaaat caacaacca gatcctgca caggaaagg gctgcacac ctcgaccga agccctgca tgccacgag ttcggcagc cctagacat gcgaaagtc aatctgcaa tttgcacga ccaaggcca cgtgagcca gaacagcga acggcgaag tctcagctc gatccatcg ctcgacgca ggagagcaa cgccagccg gcgctccca ctttgccac cagttacga gacggtgga aaggccgca gaagatcat ccgcaagga taatttatc agtcggggg gcatttcta cggccagtc ggaggccag gtcgtatgg cgagttcac caagcagca gaatgtgca aaaaattga acgggtgac tcgcaggtc caacgtttc gaatataag tttagggga cagttcttc gacgatcat cacgtcgta taagaagga gtatttgcc gcggaatgt gggcccgtg tccggcggt tttgatcga tttgcccaa aagtcccct gaagcatac gagggacac caagtcgca taaatatta cgcgccggt ttcgagtaa atgatggac tgttaacac gactctaac attgtgata cttgttatt actcataat tttattttt atagaggta ggggacggt ttttatact ttgcttggg acttttgta tcaattaat acgtgccta tgttatttt tgtatgatg caagttgta ttatcacaa agccatcaa taaattaac agtttatta
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