Status
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Gene information
General information
TC009430 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1328860..1345184
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009430
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01550

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009430 RA
      MTVNKTTKT KKKSTASTS STTASTSKV TKSTKTHGE 
      LSQASSSSS LQISDISHL PVDANIVTY TVTETLPTG 
      GGEKITVYG ETGATSTEY RHFIAQDST AKQHSATIT 
      QLSSKLNRS TSNISQESG STYTVTEPV EELKLTYNK 
      NDSGWNGKF VYEQPVTKK GQGTVVEHS STSTSHEER 
      SSIAKSSSS SYVIEIVDG KERIIDQKH HESASASSA 
      VNDEHLAMK SGTNITPEV HYKQGAKKS STKYDTAIP 
      ELTQPKTKT SESVREVHQ VGDSVSTTA HSISDSTKG 
      HKSIHHDSK SKTRDSLGK STKGEEHKV TKSEVDFSH 
      GSKTVRDES KRRESVDSN QTYTISDDK VGDLKKSSQ 
      KHFTTYHDS KTERRESVD SNQTYTISD KSDKTSDLK 
      KSSQEQFTT YYDSKTVRD EVGSKRRES LDSNQTYTI 
      SGQTSDFRQ STRGEEHAK SSDFKSTRS GVEDFTSKS 
      GDLRPKNGE LTTTTTTTY YDSKGNVIK TVQDVNTDE 
      IAGKRRDSV DSNRTYTLS EKSFAGDST DEVSKRRTA 
      QTTTDSRDF YGHGIDSSN TLVKTVYDD KAIQNTIGT 
      KGRVIKDET IDSTDLVYS NERTYGKTG WNGKFTYET 
      PRKSEAKRS PSPQKRGTS PAKKRTPSP STPKKGTSP 
      DKTAPKRGT SPVKRKVTD TVQDFLSTE ISENINQCS 
      TSDSKSTTD FQHMVPAPT DFKTFQTVS DSKTSFTSD 
      SQSFVDVKN FVETSETVD IKNVDIRDT KVSNFVDKK 
      ATVEHVTSK DVKDFRTSK DVTDRVNKV HIEENIVDI 
      KTIDEVENI TNIERFSDN KVINIVKTI KETPKPATV 
      KIPNKEQCI CELCTCGRH TCPHNPEDY NDHLHLEGP 
      FLGQPKDEY NATRGERAP IIIPKDNLK PEGDFEKRQ 
      PQKWQPGER ASVKIPEDH LKPEGEFEK RRPEEWKPG 
      DRAPVKKPK DNLRPEGDF EKRKPEPWA PGERAPVRK 
      PEDNLRPEG DFEKRKPEP WAPGDRAPV KKPQDNLKP 
      EGDFEKRKP EPWAPGERA PVRKPEDNL RPEGDFEKR 
      KPEPWAPGD RAPVKKPQD NLKPEGDFE KRKPEPWAP 
      GERAPVRKP EDNLRPEGD FEKRRPEEW RPGDRAPVK 
      KPEDNLKPE GDFEKRQPQ KWSPGERPK PKKPTDNLK 
      PEGEFEKRT PEKWAPGER EPVKKPKDN LKPEGDFEI 
      PEPEQWKPA ERSTPTKPK DNLQPEGDF ERPEPKKYQ 
      PGERPVARK PQDNLKPEG EFTGTRTII EDYRVIRGE 
      RADIKRHED NITIGGEFI DETTTKVDF NGELVPRPS 
      PIRRNTWTK IEGEFITDT TNKSEFIDY STVVERTQI 
      VKRRQDNLT VEGDMTFDT STTIDYTEK TPELEQRRR 
      RTWTKDDVD KFYSTQNTE TTTTTQEVY KQFDNQDVR 
      QTIIVHRDN LRPEGPFEG TPKSKDDFV PKYVERPKP 
      KKPEDNLRP EGDFEKRTP EKFVPAERP KQKKPQDNL 
      KPEGDFEKR TPEKFIPAE RPKQKRPED NLRPEGDFE 
      RPTPTKPEG DFERPEKPG YRPAERPQP KKPEDNLRP 
      EGDFERPEK PGYRPAERP KQKRPEDNL RPEGDFERP 
      EKPGYRPAE RPKQKKPED NLRPEGDFE RPEKPGYRP 
      AERPQPKKP EDNLRPEGD FERPEKPGY RPAERPKQK 
      RPEDNLRPE GDFERPEKP GYRPAERPQ PKKPEDNLR 
      PEGDFERPE KPGYRPAER PKQKKPEDN LRPEGDFER 
      PEKPGYRPA ERPQQKRPE DNLRPEGDF ERPEKPQYR 
      PAERPKQKK PEDNLRPEG DFERPTPTK VGPGERRTP 
      IKHDDNLRP EGDFERPEK PGYRPAERP TPKKPEDNL 
      RPEGEFERP EKPGYRPAE RPKQKRPED NLRPEGDFE 
      RPTPTKVGL AERRTPIKH DDNLRPEGD FERPEKPEY 
      RPAERPKQK RPEDNLRPE GDFERPEKP EFRPAERPK 
      QKRPEDNLR PEGDFERPT PTKVGPAER RTPIKHDDN 
      LRPEGDFER PEKPGYRPA ERPQPKRPE DNLRPEGEF 
      ERPEKPGYR PAERPKPKK PEDNLRPEG DFERPTPTK 
      VTLNVPKNL NTDRQKDRN KNVPKIILD LKVTLNGLK 
      GPSLDQLNV RNKRNRKII FVQKEILKG RLQLKLSQL 
      NVGHLLSTM IIYVLKEIS NVLRSLSTD RLKDLNLRN 
      LKIIFILKA ILRDQLQPR LDQLKEEHQ LSMGIIFGL 
      KEILNGLKN PNIDQLKDL NLKSLKIIF GLKAILKDR 
      LQPRLVLLK EEHLSSTMI IFDLKETSK DPKNLNSDQ 
      LNVRNLKNL KIIFALKVI LKDQPQPRL VLLKEGHQL 
      SMVIIFGLK VTSNVLKNL NTDQLKDPN LRNLKIIFA 
      QKATLKGQH LPRLDQLKK EHLLSTMII YVLKEISNV 
      LKSLSTGRL KDPNLRNLK IIFVLKAIL KDRRQPRLD 
      QLKGEHQLS MGIIFGLKE ILNGLKNPN IDQLKDLSL 
      KSLKTIFVL KVISKGQHQ PKLVLLKGE HQLDTVTIS 
      DLKETLKDP KNLNLNQLN VRNLKNPKI IFVLKANSK 
      DQVQQKLDQ LKEEHQLNM VIISDLKET SNDLKSLNI 
      DRLKDPNLK NPKIIFVQK EILKDRLQP KLVLLKEEH 
      QLDTETISD LKETSNDPK NLNSDQLNV RNLKNPKIT 
      FVLKVNSKD QVQQKPEKP KFKPAERPQ PKKPEDNLR 
      PEGEFERPE LIEVGPCEL RKPIKHEDN LKPEGTLVV 
      TRKDDYNVV KGERVDVIK REDNLRVEG EWDIRRRDD 
      YSNTTVVDR VDVIKRQDN LRVEGEWDI RRRDDYSNT 
      TVVDRVDVI KRQDNLRVE GEFTDVRTR DDYKVVKGE 
      RYDTVIHQD NLKTEGEFR SSRTSDEYS VVKGERAEI 
      ITRQDNLRM EGEFNEYRK RDEFNTRIV EKAEVIRHQ 
      DNLKLEGTF ERPSHVHLG PGERRKPIK HEDNLHPEG 
      DFERPDRPG FKPAERPSP IKPQDNLKP EGKFELPTS 
      PGFQPGERR TPIRHQDNL HPEGDFERP QRPQYQPIE 
      RPTPKKPKD NLKPEGTFE RPTASPVGP GERRSPIRH 
      HDNLRPEGA FERPDKPGY KPGERPTPK KPQDNLRPE 
      GEFEKRTPA KPQPGERRT PIRHQDNLH PEGAFERPE 
      KPKYSPGER LPMKKPQDN LRPEGEFET PQKPKYGPG 
      ERAPIVKHP DNLKPEGEF IGTPKSKDD FKPTRGERA 
      PVKKPQDNL KITGEFQDM TMQKSQFVV TRGERAEIR 
      KHEDSLTIG NGKTEYITT SKETFDKTT PTKVVTSPA 
      TPGSINRRR HIESHITLG SDTAVMQTT NQSNYNTYT 
      KKIDRQSTT GRNEVANKT TNVATDSKT LADGTTVTT 
      TRTTTKTTG QQLAKQTQQ VSAEVSTHH RKNVITSEE 
      INNQILHRK GLHTSTEAL HATSSAALD MRKSICNLH 
      DQGHVSQNS ERRSLSSIH RSTQESNAS RRSHFATSY 
      ETVERPQKI IRKDNLSVG GHFYGQSEA RSYGEFTKQ 
      QNVQKIERV TRRSNVSNI SLGDSSSTI ITSYKKEYL 
      PRNVGPCPA VLIDLPKSP LKHTRDTKS HKYYAPVSS 
      K
    CDS sequence
    • >TC009430 RA
      atgacggtg aataagaca accaagacg aaaaaaaag 
      agcacagct tcgacctcc tcgacaacg gcaagtacc 
      tccaaagtc accaaatcg acaaaaaca cacggcgag 
      ctatcgcaa gcctcctct tcgtccagt ttgcaaatt 
      tccgatatt tcgcattta ccggttgac gctaacatc 
      gtcacatac acggtaacg gagacttta ccgaccgga 
      ggcggcgag aaaatcaca gtgtatggc gaaactggc 
      gccacaagc actgaatac cgccacttt atcgcccaa 
      gattcgaca gcgaaacaa cactccgcc actatcaca 
      caactcagc tcgaaacta aaccgctcc acttcgaac 
      atatcgcaa gaatccggc tccacgtac actgtaacc 
      gaaccggtg gaagaactc aaactaact tacaataaa 
      aacgattct ggatggaac gggaagttc gtttacgaa 
      caacctgtc acaaaaaag ggacaaggg acagttgtc 
      gaacactcc tccacgtcc acgtcacac gaggaacgt 
      tcttcaatt gccaaaagt tcgtccagt agttacgtt 
      atcgaaata gtcgatggt aaagaacgc ataattgac 
      cagaaacac catgagtcc gccagtgcg tcttctgca 
      gtcaatgac gaacatttg gccatgaaa agtggcact 
      aacatcaca ccagaagtg cactacaag caaggggcg 
      aagaaaagt tcgacaaag tatgatact gctattcct 
      gaactaaca caacccaaa actaaaact tccgaatct 
      gtgagagaa gtgcaccag gtcggagac tccgtttcg 
      acgacagct cactccatt agcgactcg acaaaagga 
      cacaaatcg atccatcac gattccaaa agcaaaaca 
      agagattcc ttaggaaag tcaacaaag ggggaagaa 
      cataaagtg acaaagagc gaagttgat ttctctcac 
      ggttccaaa accgtgaga gatgagagc aagagaaga 
      gaatcagtt gattcgaac caaacctac accattagt 
      gatgacaaa gttggcgac ttgaaaaaa tcgtcccaa 
      aaacatttc acaacttat cacgattcc aaaactgag 
      agaagagag tcagttgat tcgaaccaa acctacacc 
      attagtgac aaaagcgat aaaacaagt gacttgaaa 
      aagtcgtca caagaacag ttcacaact tattacgac 
      tccaaaact gtaagagat gaagttggt agtaagagg 
      agagaatca cttgattcg aaccaaacc tacaccatt 
      agtggccaa actagcgac ttccgacag tcaacaaga 
      ggcgaagag cacgccaag tctagtgat ttcaagtct 
      acaagaagt ggagtggaa gatttcacc tccaaatct 
      ggcgatctt cgcccaaaa aacggcgaa ctgaccacc 
      accaccact accacctac tacgattct aaaggcaat 
      gtgataaag acagtccag gacgtgaac actgatgaa 
      atcgccggt aagaggcgt gattcagtc gactcaaac 
      cgcacctac accctgagc gagaaaagt ttcgccggc 
      gattcaacc gacgaagtc tcaaaacgg cgaactgct 
      cagaccacc accgattcg cgcgacttt tacggccat 
      gggatcgat tcgtcaaac actctcgtg aagacagtt 
      tacgacgat aaagccatc cagaacaca atcggtacc 
      aaaggcaga gtaatcaag gacgaaacg atcgattcg 
      accgacttg gtctattca aacgaacgc acttatgga 
      aaaacaggc tggaatggg aagtttacc tacgaaacg 
      cccagaaag agtgaagct aaacgctcg ccctcgccc 
      caaaaacgg ggcacttcg ccggcgaaa aagcgaaca 
      ccgtcgcct tcgacacct aaaaaggga acttcgccg 
      gataaaacg gcaccgaaa aggggaact tcgccggtg 
      aagcgaaaa gtgactgat accgtccag gatttttta 
      tcgactgaa atcagtgag aacatcaac cagtgcagt 
      acaagtgat tcgaaaagt accacggat ttccaacac 
      atggtgcca gctcctact gatttcaag accttccaa 
      acggtctca gactcgaaa actagcttc acttcggac 
      agccaaagt tttgtcgat gtgaaaaat ttcgttgag 
      acaagtgag actgtcgat atcaaaaat gttgatatc 
      agggacacg aaagtgagc aactttgtg gacaagaaa 
      gcgaccgtc gaacatgtg accagtaag gatgttaag 
      gattttcgc acgtccaag gatgtcaca gatagggtt 
      aataaagtg cacattgag gagaacatt gtcgatatt 
      aagaccatt gacgaggtc gagaatatc acaaatatt 
      gaacggttc agcgataat aaagttata aatattgtg 
      aagaccatc aaagaaaca ccgaagcct gcaactgtt 
      aagattccc aataaggag caatgtatc tgcgagttg 
      tgcacgtgt ggacgtcac acgtgtcct cacaacccc 
      gaagactac aacgatcac ttacacctc gaaggcccc 
      tttctaggc caacctaaa gacgaatac aatgcaacc 
      agaggcgaa agagcccca attatcata cctaaagac 
      aacctaaaa ccagaaggg gattttgaa aaacgccag 
      ccacaaaaa tggcaacct ggcgaaaga gcctctgta 
      aaaataccg gaagaccac ctaaaacct gaaggagaa 
      tttgaaaag agacgacct gaagaatgg aaacctggc 
      gatcgtgcg cctgtgaaa aaacctaaa gataatcta 
      cgacctgaa ggagatttc gaaaaacgc aaacctgaa 
      ccctgggca cctggagaa agggctcca gtgcgcaaa 
      cctgaagat aatctaaga ccagaaggc gattttgaa 
      aaacgcaaa cctgaacct tgggcacct ggtgatcgg 
      gcgcccgtt aagaaaccc caagacaac ctaaaaccg 
      gaaggtgat tttgaaaaa cggaagcct gaaccttgg 
      gcacctgga gaaagggct ccagtgcgc aaacctgaa 
      gacaaccta agaccggag ggtgatttt gaaaaacgg 
      aagcctgaa ccttgggca cctggcgac cgggcgccc 
      gttaagaaa ccccaagac aacctaaaa ccggaaggt 
      gattttgaa aaacggaag cctgaacct tgggcacct 
      ggagaaagg gcgccagtg cgcaaacct gaagacaac 
      ctaagacca gaaggcgat tttgaaaaa cgccgcccc 
      gaggaatgg agacctggc gatcgggcg cccgttaaa 
      aaacctgaa gacaaccta aaacctgaa ggggatttc 
      gaaaaacgc caacctcag aaatggtca cctggtgaa 
      cgaccaaaa cctaaaaaa cctaccgat aatttgaaa 
      cctgaaggc gagtttgag aaacgaacg cctgaaaaa 
      tgggctcct ggggagcga gaaccggtg aagaaacct 
      aaagataat ttgaaacct gagggcgat tttgaaata 
      cctgagcct gagcagtgg aaacctgcc gaaaggtct 
      acgcctacc aaacctaaa gataatctg caacctgag 
      ggtgatttt gaacgacct gagcctaag aaatatcaa 
      ccgggtgaa aggccagtt gcaaggaag ccgcaggat 
      aatttgaaa cctgaaggt gaatttact gggactagg 
      accattatt gaggattat cgtgtgatt cggggagaa 
      cgcgccgat atcaagcgt catgaagac aacatcact 
      ataggaggt gaatttata gacgaaact accactaaa 
      gttgatttt aatggcgag ttagtacca cgtcctagc 
      ccaatccga cgcaacact tggacgaaa atcgagggc 
      gaatttatc acagatacg accaataaa tccgaattt 
      attgattac agtactgtt gttgaaaga acacaaatt 
      gtgaaaaga cggcaagat aatctcact gttgaggga 
      gacatgact tttgatact tcaaccacg atcgattat 
      acagaaaaa acacccgaa ctcgaacaa aggcgtcgc 
      agaacttgg acgaaagat gacgtcgat aaattctat 
      tcaacgcaa aacactgaa acaacaaca acaacgcaa 
      gaagtttac aaacagttt gataaccaa gacgttcgc 
      caaacaatt attgtacac agagataac ctcaggccg 
      gagggacct tttgaagga acaccaaaa tcaaaggat 
      gacttcgtc cctaaatat gttgaacgt cctaaaccg 
      aaaaaacct gaagataat ttaagacca gagggtgat 
      tttgaaaaa cgcacacct gagaaattt gttcctgcg 
      gaacgacct aagcaaaag aaaccgcaa gataatttg 
      aaacctgaa ggcgatttt gagaaacgc actcccgag 
      aaatttatt ccagccgaa aggcctaaa cagaagaga 
      cctgaagat aatcttcgc ccagaaggt gattttgaa 
      agacctact ccgaccaag cctgagggt gattttgaa 
      agacccgaa aagcctgga tacaggcct gcagaacgt 
      ccacaacct aaaaaacct gaagataat ttaaggcct 
      gagggagat tttgaacgt ccagagaaa cctggatat 
      cgtccagct gagcgtcca aaacagaaa cgtcctgag 
      gataatttg aggcctgag ggagatttt gaaagacct 
      gaaaaacct ggatatcgc ccagctgaa cgaccaaaa 
      cagaaaaaa cctgaggat aatttgagg cctgaaggt 
      gactttgaa agacctgaa aagcctgga tataggcct 
      gcagaacga ccacaaccg aaaaaacct gaagataac 
      ttgcggcct gaaggagat tttgaacgt ccagagaaa 
      cctggatat cgtccagct gaacgtcca aaacaaaag 
      agacctgag gataatttg aggcctgaa ggtgacttt 
      gaaagacct gaaaaacct ggatatagg cctgcagaa 
      cgtccacag cctaaaaaa cctgaagat aatttgcgg 
      cctgaagga gattttgaa cgtccagag aaacctgga 
      tatcgtcca gctgagcgt cctaaacag aagaaacct 
      gaggataat ttgaggcct gagggagat tttgaaagg 
      cctgaaaaa cctggatat cgcccagct gaacgtcct 
      caacaaaaa cgccctgaa gataatcta agaccagaa 
      ggtgatttt gaaaggccg gaaaaaccg caatatcgt 
      ccagctgag cgtccaaaa cagaagaaa cctgaagat 
      aatctcaga cctgagggc gactttgaa agaccaacc 
      ccaaccaag gttggtcca ggtgaaaga agaacacct 
      attaagcat gacgataac cttagacct gaaggtgat 
      tttgaacgt ccagaaaag cctggatat cgtcctgct 
      gaacgtcca actcctaaa aaacctgaa gataatctt 
      cggccagaa ggtgaattt gagaggccg gagaaacct 
      ggatatcgt ccagcagaa cgtccgaaa caaaagcgt 
      ccggaagat aatttaagg cctgaagga gattttgaa 
      agaccaaca cctacgaaa gttgggctg gcagaacgc 
      agaactcca attaaacat gatgataat ttgagacct 
      gaaggtgac tttgaacgt cctgaaaaa cctgaatac 
      cgaccggca gaaagaccg aaacaaaaa cgtcctgaa 
      gacaatctt aggcctgaa ggtgatttt gaacggccg 
      gaaaaaccc gaatttagg ccagcagaa cgtcctaaa 
      cagaaaaga cctgaagat aatcttcgt ccagaagga 
      gattttgaa agaccgact ccaactaaa gttgggcca 
      gctgaacgt aggacacct atcaagcat gatgataat 
      ctacgtcct gaaggagat ttcgaacgt cctgaaaaa 
      cctggatat aggcctgca gaacggcca caacctaaa 
      aggcctgaa gataattta aggcctgaa ggtgaattt 
      gagaggccg gagaaacct ggatatcgt ccagctgaa 
      cgtccgaaa cctaagaaa cctgaagat aatctccga 
      cctgaagga gattttgaa agacctact cctacgaaa 
      gtgactttg aacgtcccg aaaaacctg aataccgac 
      cggcagaaa gaccgaaac aaaaacgtc ccgaagata 
      atcttagac ctgaaggtg actttgaac ggcctgaaa 
      ggcccgagt ttagaccag ctgaacgtc cgaaacaaa 
      agaaaccgg aagataatc ttcgtccag aaggagatt 
      ttgaaaggc cgactccaa ctaaagttg agccagctg 
      aacgtagga cacctatta agcacgatg ataatctac 
      gtcctgaag gagatttcg aacgtcctg agaagcctc 
      agtacagac cggctgaaa gacctcaac ctaagaaac 
      ctgaagata atcttcatc ctgaaggcg attttgaga 
      gaccaactc caaccaagg ttggaccag ctgaaagaa 
      gaacaccaa ttaagcatg gggataatc ttcggcctg 
      aaggagatt ttgaacggc ctgaaaaac cccaatata 
      gaccagctg aaagacctc aacctaaaa agcctgaag 
      ataatcttc ggcctgaag gcgattttg aaagaccga 
      ctccaacca aggttggtc ctgctgaaa gaagaacac 
      ctatcaagc acgatgata atcttcgac ctgaaggag 
      acttcgaaa gacccgaaa aacctgaat tcagaccag 
      ctgaacgtc cgaaaccta aaaaacctg aagataatc 
      ttcgccctg aaggtgatt ttgaaagac caaccccaa 
      ccaaggttg gtcctgctg aaagaagga caccaatta 
      agcatggtg ataatcttc ggcctgaag gtgacttcg 
      aacgtcctg aaaaacctc aatacagac cagctgaaa 
      gaccccaac ctaagaaac ctgaagata atcttcgcc 
      cagaaggcg actttgaaa ggccaacac ctaccaagg 
      ttggaccag ctgaaaaaa gaacaccta ttaagcacg 
      atgataatt tacgtcctg aaggagatt tcgaacgtc 
      ctgaaaagc ctcagtaca ggccggctg aaagacccc 
      aacctaaga aacctgaag ataatcttc gtcctgaag 
      gcgattttg aaagaccga cgccaacca aggttggac 
      cagctgaaa ggagaacac caattaagc atggggata 
      atcttcggc ctgaaggag attttgaac ggcctgaaa 
      aaccccaat atagaccag ctgaaagac ctcagccta 
      aaaagcctg aagacaatc ttcgtcctg aaggtgatt 
      tcgaaaggc caacaccaa ccaaagttg gtcctgctg 
      aaaggagaa caccaatta gacacggtg acaatctcc 
      gacctgaag gagactttg aaagacccg aaaaacctg 
      aatttaaac cagctgaac gtccgaaac ctaaaaaac 
      ccgaagata atcttcgtc ctgaaggcg aattcgaaa 
      gaccaagtc caacaaaag ttggaccag ctgaaagaa 
      gaacaccaa ttaaacatg gtgataatc tccgacctg 
      aaggagact tcgaacgac ctgaaaagc ctcaatata 
      gaccggctg aaagacccc aacctaaaa aacccgaag 
      ataatcttc gtccagaag gagattttg aaagaccga 
      ctccaacca aagttggtc ctgctgaaa gaagaacac 
      caattagac acggagaca atctccgac ctgaaggag 
      acttcgaac gacccgaaa aacctgaat tcagaccag 
      ctgaacgtc cgaaaccta aaaaacccg aagataacc 
      ttcgtcctg aaggtgaat tcgaaagac caagtccaa 
      caaaaaccc gaaaaaccc aaattcaaa ccagctgaa 
      cgtccccaa cctaaaaaa cctgaagac aatcttcgt 
      cctgaaggt gaattcgaa cggcctgag ctaatagaa 
      gtgggcccg tgtgaattg cgcaaacct atcaaacat 
      gaagataat ctcaaacct gaaggaaca cttgttgtt 
      accagaaag gacgattac aacgttgtg aaaggcgaa 
      cgagttgat gtgatcaaa cgtgaagat aatttacgt 
      gtcgaagga gaatgggat attcggcga agagacgat 
      tacagtaac actacagtc gtagatcgg gtcgatgtt 
      atcaaacgc caagacaat ctacgtgtt gaaggagaa 
      tgggatatt agacgtaga gatgactac agtaataca 
      acggtcgta gatcgcgtc gatgtcatc aaacgccaa 
      gacaattta cgcgtcgag ggcgaattc accgatgta 
      cgaacacga gatgactac aaagtcgtc aagggcgaa 
      agatacgat actgtcatt caccaagac aatctaaaa 
      acagaagga gaattccga agtagtcgc actagcgac 
      gaatactcc gttgtcaaa ggcgaacgc gcagaaatc 
      atcaccaga caagacaat ctccgaatg gaaggcgaa 
      ttcaacgaa taccgaaaa cgcgacgaa ttcaacact 
      cgaattgtc gaaaaagct gaagtgatc cgccaccaa 
      gacaacctg aaactggaa ggcaccttc gaacgcccg 
      tcccacgtc cacctagga cctggcgaa cgtcgcaaa 
      ccaatcaaa cacgaagac aacctccac cctgaaggc 
      gacttcgaa cgcccagac cgccccggc tttaaacca 
      gccgaaagg ccgtcacca atcaaaccc caagacaat 
      ctcaaacct gaaggaaaa ttcgaacta ccaacttcc 
      cccggtttc caacccggc gaacgtcgg actccgatt 
      cgccaccag gataacttg cacccagaa ggcgacttc 
      gaacgtccc caaagacca caataccaa ccaatcgaa 
      cgcccaaca cccaaaaaa cccaaagac aaccttaaa 
      cccgaagga acattcgaa cgtccaacg gcaagtccc 
      gtgggaccc ggcgaacgg cgaagccca atccgccac 
      catgacaat ttaagacct gaaggtgcg ttcgaacgc 
      ccagacaag cccggctat aaaccaggc gaacgccca 
      actccgaaa aaaccccag gataaccta cgcccagaa 
      ggggaattc gaaaaacga accccggcg aaaccacaa 
      cctggcgaa cggcgaact ccgatccgc caccaggat 
      aatctgcac cctgagggt gctttcgag cgacccgag 
      aagcctaaa tactcgcct ggtgaacgc ctaccaatg 
      aagaaaccc caagataat ttgagaccc gaaggtgag 
      tttgaaacc ccccagaaa cctaaatac gggcctggc 
      gaacgtgcc cccatcgtc aaacaccca gacaacctt 
      aaacctgaa ggcgaattc atcggaaca cccaaaagt 
      aaagacgat tttaaacca actcggggc gaacgagct 
      ccggttaag aaaccacaa gacaatttg aaaatcaca 
      ggagagttc caagacatg acaatgcag aaatcacag 
      tttgtcgta acccgaggt gagagagca gaaatacgc 
      aaacatgaa gattcccta actataggg aacggcaaa 
      actgaatat atcaccaca tcgaaggaa acgtttgat 
      aaaacaacg cccacgaaa gtggtcaca agtccagca 
      actcctgga tccatcaac aggagacgc cacattgag 
      tcacacatt actctaggg agtgacaca gcagtgatg 
      caaacaacg aaccaaagc aactacaac acttacacc 
      aagaaaatt gatcgccag tcaacaaca ggccgtaac 
      gaagttgca aataaaaca acgaacgtt gcaacggat 
      agtaagact ttggctgac ggtacaact gtcacaaca 
      actcgcaca acaaccaaa acaacagga caacaactg 
      gcgaaacaa acccagcaa gtctccgcc gaagtgtca 
      acccaccac cgcaaaaac gtcataact tcggaggaa 
      atcaacaac cagatcctg cacaggaaa gggctgcac 
      acctcgacc gaagccctg catgccacg agttcggca 
      gccctagac atgcgaaag tcaatctgc aatttgcac 
      gaccaaggc cacgtgagc cagaacagc gaacggcga 
      agtctcagc tcgatccat cgctcgacg caggagagc 
      aacgccagc cggcgctcc cactttgcc accagttac 
      gagacggtg gaaaggccg cagaagatc atccgcaag 
      gataattta tcagtcggg gggcatttc tacggccag 
      tcggaggcc aggtcgtat ggcgagttc accaagcag 
      cagaatgtg caaaaaatt gaacgggtg actcgcagg 
      tccaacgtt tcgaatata agtttaggg gacagttct 
      tcgacgatc atcacgtcg tataagaag gagtatttg 
      ccgcggaat gtgggcccg tgtccggcg gttttgatc 
      gatttgccc aaaagtccc ctgaagcat acgagggac 
      accaagtcg cataaatat tacgcgccg gtttcgagt 
      aaatga
    mRNA sequence
    • >TC009430 RA
      tcactttca aaaagagcg cgaccgcga acgcacgca 
      ccgccaata tttgtgtta ttaactttg tgtgaaaat 
      gacggtgaa taagacaac caagacgaa aaaaaagag 
      cacagcttc gacctcctc gacaacggc aagtacctc 
      caaagtcac caaatcgac aaaaacaca cggcgagct 
      atcgcaagc ctcctcttc gtccagttt gcaaatttc 
      cgatatttc gcatttacc ggttgacgc taacatcgt 
      cacatacac ggtaacgga gactttacc gaccggagg 
      cggcgagaa aatcacagt gtatggcga aactggcgc 
      cacaagcac tgaataccg ccactttat cgcccaaga 
      ttcgacagc gaaacaaca ctccgccac tatcacaca 
      actcagctc gaaactaaa ccgctccac ttcgaacat 
      atcgcaaga atccggctc cacgtacac tgtaaccga 
      accggtgga agaactcaa actaactta caataaaaa 
      cgattctgg atggaacgg gaagttcgt ttacgaaca 
      acctgtcac aaaaaaggg acaagggac agttgtcga 
      acactcctc cacgtccac gtcacacga ggaacgttc 
      ttcaattgc caaaagttc gtccagtag ttacgttat 
      cgaaatagt cgatggtaa agaacgcat aattgacca 
      gaaacacca tgagtccgc cagtgcgtc ttctgcagt 
      caatgacga acatttggc catgaaaag tggcactaa 
      catcacacc agaagtgca ctacaagca aggggcgaa 
      gaaaagttc gacaaagta tgatactgc tattcctga 
      actaacaca acccaaaac taaaacttc cgaatctgt 
      gagagaagt gcaccaggt cggagactc cgtttcgac 
      gacagctca ctccattag cgactcgac aaaaggaca 
      caaatcgat ccatcacga ttccaaaag caaaacaag 
      agattcctt aggaaagtc aacaaaggg ggaagaaca 
      taaagtgac aaagagcga agttgattt ctctcacgg 
      ttccaaaac cgtgagaga tgagagcaa gagaagaga 
      atcagttga ttcgaacca aacctacac cattagtga 
      tgacaaagt tggcgactt gaaaaaatc gtcccaaaa 
      acatttcac aacttatca cgattccaa aactgagag 
      aagagagtc agttgattc gaaccaaac ctacaccat 
      tagtgacaa aagcgataa aacaagtga cttgaaaaa 
      gtcgtcaca agaacagtt cacaactta ttacgactc 
      caaaactgt aagagatga agttggtag taagaggag 
      agaatcact tgattcgaa ccaaaccta caccattag 
      tggccaaac tagcgactt ccgacagtc aacaagagg 
      cgaagagca cgccaagtc tagtgattt caagtctac 
      aagaagtgg agtggaaga tttcacctc caaatctgg 
      cgatcttcg cccaaaaaa cggcgaact gaccaccac 
      caccactac cacctacta cgattctaa aggcaatgt 
      gataaagac agtccagga cgtgaacac tgatgaaat 
      cgccggtaa gaggcgtga ttcagtcga ctcaaaccg 
      cacctacac cctgagcga gaaaagttt cgccggcga 
      ttcaaccga cgaagtctc aaaacggcg aactgctca 
      gaccaccac cgattcgcg cgactttta cggccatgg 
      gatcgattc gtcaaacac tctcgtgaa gacagttta 
      cgacgataa agccatcca gaacacaat cggtaccaa 
      aggcagagt aatcaagga cgaaacgat cgattcgac 
      cgacttggt ctattcaaa cgaacgcac ttatggaaa 
      aacaggctg gaatgggaa gtttaccta cgaaacgcc 
      cagaaagag tgaagctaa acgctcgcc ctcgcccca 
      aaaacgggg cacttcgcc ggcgaaaaa gcgaacacc 
      gtcgccttc gacacctaa aaagggaac ttcgccgga 
      taaaacggc accgaaaag gggaacttc gccggtgaa 
      gcgaaaagt gactgatac cgtccagga ttttttatc 
      gactgaaat cagtgagaa catcaacca gtgcagtac 
      aagtgattc gaaaagtac cacggattt ccaacacat 
      ggtgccagc tcctactga tttcaagac cttccaaac 
      ggtctcaga ctcgaaaac tagcttcac ttcggacag 
      ccaaagttt tgtcgatgt gaaaaattt cgttgagac 
      aagtgagac tgtcgatat caaaaatgt tgatatcag 
      ggacacgaa agtgagcaa ctttgtgga caagaaagc 
      gaccgtcga acatgtgac cagtaagga tgttaagga 
      ttttcgcac gtccaagga tgtcacaga tagggttaa 
      taaagtgca cattgagga gaacattgt cgatattaa 
      gaccattga cgaggtcga gaatatcac aaatattga 
      acggttcag cgataataa agttataaa tattgtgaa 
      gaccatcaa agaaacacc gaagcctgc aactgttaa 
      gattcccaa taaggagca atgtatctg cgagttgtg 
      cacgtgtgg acgtcacac gtgtcctca caaccccga 
      agactacaa cgatcactt acacctcga aggcccctt 
      tctaggcca acctaaaga cgaatacaa tgcaaccag 
      aggcgaaag agccccaat tatcatacc taaagacaa 
      cctaaaacc agaagggga ttttgaaaa acgccagcc 
      acaaaaatg gcaacctgg cgaaagagc ctctgtaaa 
      aataccgga agaccacct aaaacctga aggagaatt 
      tgaaaagag acgacctga agaatggaa acctggcga 
      tcgtgcgcc tgtgaaaaa acctaaaga taatctacg 
      acctgaagg agatttcga aaaacgcaa acctgaacc 
      ctgggcacc tggagaaag ggctccagt gcgcaaacc 
      tgaagataa tctaagacc agaaggcga ttttgaaaa 
      acgcaaacc tgaaccttg ggcacctgg tgatcgggc 
      gcccgttaa gaaacccca agacaacct aaaaccgga 
      aggtgattt tgaaaaacg gaagcctga accttgggc 
      acctggaga aagggctcc agtgcgcaa acctgaaga 
      caacctaag accggaggg tgattttga aaaacggaa 
      gcctgaacc ttgggcacc tggcgaccg ggcgcccgt 
      taagaaacc ccaagacaa cctaaaacc ggaaggtga 
      ttttgaaaa acggaagcc tgaaccttg ggcacctgg 
      agaaagggc gccagtgcg caaacctga agacaacct 
      aagaccaga aggcgattt tgaaaaacg ccgccccga 
      ggaatggag acctggcga tcgggcgcc cgttaaaaa 
      acctgaaga caacctaaa acctgaagg ggatttcga 
      aaaacgcca acctcagaa atggtcacc tggtgaacg 
      accaaaacc taaaaaacc taccgataa tttgaaacc 
      tgaaggcga gtttgagaa acgaacgcc tgaaaaatg 
      ggctcctgg ggagcgaga accggtgaa gaaacctaa 
      agataattt gaaacctga gggcgattt tgaaatacc 
      tgagcctga gcagtggaa acctgccga aaggtctac 
      gcctaccaa acctaaaga taatctgca acctgaggg 
      tgattttga acgacctga gcctaagaa atatcaacc 
      gggtgaaag gccagttgc aaggaagcc gcaggataa 
      tttgaaacc tgaaggtga atttactgg gactaggac 
      cattattga ggattatcg tgtgattcg gggagaacg 
      cgccgatat caagcgtca tgaagacaa catcactat 
      aggaggtga atttataga cgaaactac cactaaagt 
      tgattttaa tggcgagtt agtaccacg tcctagccc 
      aatccgacg caacacttg gacgaaaat cgagggcga 
      atttatcac agatacgac caataaatc cgaatttat 
      tgattacag tactgttgt tgaaagaac acaaattgt 
      gaaaagacg gcaagataa tctcactgt tgagggaga 
      catgacttt tgatacttc aaccacgat cgattatac 
      agaaaaaac acccgaact cgaacaaag gcgtcgcag 
      aacttggac gaaagatga cgtcgataa attctattc 
      aacgcaaaa cactgaaac aacaacaac aacgcaaga 
      agtttacaa acagtttga taaccaaga cgttcgcca 
      aacaattat tgtacacag agataacct caggccgga 
      gggaccttt tgaaggaac accaaaatc aaaggatga 
      cttcgtccc taaatatgt tgaacgtcc taaaccgaa 
      aaaacctga agataattt aagaccaga gggtgattt 
      tgaaaaacg cacacctga gaaatttgt tcctgcgga 
      acgacctaa gcaaaagaa accgcaaga taatttgaa 
      acctgaagg cgattttga gaaacgcac tcccgagaa 
      atttattcc agccgaaag gcctaaaca gaagagacc 
      tgaagataa tcttcgccc agaaggtga ttttgaaag 
      acctactcc gaccaagcc tgagggtga ttttgaaag 
      acccgaaaa gcctggata caggcctgc agaacgtcc 
      acaacctaa aaaacctga agataattt aaggcctga 
      gggagattt tgaacgtcc agagaaacc tggatatcg 
      tccagctga gcgtccaaa acagaaacg tcctgagga 
      taatttgag gcctgaggg agattttga aagacctga 
      aaaacctgg atatcgccc agctgaacg accaaaaca 
      gaaaaaacc tgaggataa tttgaggcc tgaaggtga 
      ctttgaaag acctgaaaa gcctggata taggcctgc 
      agaacgacc acaaccgaa aaaacctga agataactt 
      gcggcctga aggagattt tgaacgtcc agagaaacc 
      tggatatcg tccagctga acgtccaaa acaaaagag 
      acctgagga taatttgag gcctgaagg tgactttga 
      aagacctga aaaacctgg atataggcc tgcagaacg 
      tccacagcc taaaaaacc tgaagataa tttgcggcc 
      tgaaggaga ttttgaacg tccagagaa acctggata 
      tcgtccagc tgagcgtcc taaacagaa gaaacctga 
      ggataattt gaggcctga gggagattt tgaaaggcc 
      tgaaaaacc tggatatcg cccagctga acgtcctca 
      acaaaaacg ccctgaaga taatctaag accagaagg 
      tgattttga aaggccgga aaaaccgca atatcgtcc 
      agctgagcg tccaaaaca gaagaaacc tgaagataa 
      tctcagacc tgagggcga ctttgaaag accaacccc 
      aaccaaggt tggtccagg tgaaagaag aacacctat 
      taagcatga cgataacct tagacctga aggtgattt 
      tgaacgtcc agaaaagcc tggatatcg tcctgctga 
      acgtccaac tcctaaaaa acctgaaga taatcttcg 
      gccagaagg tgaatttga gaggccgga gaaacctgg 
      atatcgtcc agcagaacg tccgaaaca aaagcgtcc 
      ggaagataa tttaaggcc tgaaggaga ttttgaaag 
      accaacacc tacgaaagt tgggctggc agaacgcag 
      aactccaat taaacatga tgataattt gagacctga 
      aggtgactt tgaacgtcc tgaaaaacc tgaataccg 
      accggcaga aagaccgaa acaaaaacg tcctgaaga 
      caatcttag gcctgaagg tgattttga acggccgga 
      aaaacccga atttaggcc agcagaacg tcctaaaca 
      gaaaagacc tgaagataa tcttcgtcc agaaggaga 
      ttttgaaag accgactcc aactaaagt tgggccagc 
      tgaacgtag gacacctat caagcatga tgataatct 
      acgtcctga aggagattt cgaacgtcc tgaaaaacc 
      tggatatag gcctgcaga acggccaca acctaaaag 
      gcctgaaga taatttaag gcctgaagg tgaatttga 
      gaggccgga gaaacctgg atatcgtcc agctgaacg 
      tccgaaacc taagaaacc tgaagataa tctccgacc 
      tgaaggaga ttttgaaag acctactcc tacgaaagt 
      gactttgaa cgtcccgaa aaacctgaa taccgaccg 
      gcagaaaga ccgaaacaa aaacgtccc gaagataat 
      cttagacct gaaggtgac tttgaacgg cctgaaagg 
      cccgagttt agaccagct gaacgtccg aaacaaaag 
      aaaccggaa gataatctt cgtccagaa ggagatttt 
      gaaaggccg actccaact aaagttgag ccagctgaa 
      cgtaggaca cctattaag cacgatgat aatctacgt 
      cctgaagga gatttcgaa cgtcctgag aagcctcag 
      tacagaccg gctgaaaga cctcaacct aagaaacct 
      gaagataat cttcatcct gaaggcgat tttgagaga 
      ccaactcca accaaggtt ggaccagct gaaagaaga 
      acaccaatt aagcatggg gataatctt cggcctgaa 
      ggagatttt gaacggcct gaaaaaccc caatataga 
      ccagctgaa agacctcaa cctaaaaag cctgaagat 
      aatcttcgg cctgaaggc gattttgaa agaccgact 
      ccaaccaag gttggtcct gctgaaaga agaacacct 
      atcaagcac gatgataat cttcgacct gaaggagac 
      ttcgaaaga cccgaaaaa cctgaattc agaccagct 
      gaacgtccg aaacctaaa aaacctgaa gataatctt 
      cgccctgaa ggtgatttt gaaagacca accccaacc 
      aaggttggt cctgctgaa agaaggaca ccaattaag 
      catggtgat aatcttcgg cctgaaggt gacttcgaa 
      cgtcctgaa aaacctcaa tacagacca gctgaaaga 
      ccccaacct aagaaacct gaagataat cttcgccca 
      gaaggcgac tttgaaagg ccaacacct accaaggtt 
      ggaccagct gaaaaaaga acacctatt aagcacgat 
      gataattta cgtcctgaa ggagatttc gaacgtcct 
      gaaaagcct cagtacagg ccggctgaa agaccccaa 
      cctaagaaa cctgaagat aatcttcgt cctgaaggc 
      gattttgaa agaccgacg ccaaccaag gttggacca 
      gctgaaagg agaacacca attaagcat ggggataat 
      cttcggcct gaaggagat tttgaacgg cctgaaaaa 
      ccccaatat agaccagct gaaagacct cagcctaaa 
      aagcctgaa gacaatctt cgtcctgaa ggtgatttc 
      gaaaggcca acaccaacc aaagttggt cctgctgaa 
      aggagaaca ccaattaga cacggtgac aatctccga 
      cctgaagga gactttgaa agacccgaa aaacctgaa 
      tttaaacca gctgaacgt ccgaaacct aaaaaaccc 
      gaagataat cttcgtcct gaaggcgaa ttcgaaaga 
      ccaagtcca acaaaagtt ggaccagct gaaagaaga 
      acaccaatt aaacatggt gataatctc cgacctgaa 
      ggagacttc gaacgacct gaaaagcct caatataga 
      ccggctgaa agaccccaa cctaaaaaa cccgaagat 
      aatcttcgt ccagaagga gattttgaa agaccgact 
      ccaaccaaa gttggtcct gctgaaaga agaacacca 
      attagacac ggagacaat ctccgacct gaaggagac 
      ttcgaacga cccgaaaaa cctgaattc agaccagct 
      gaacgtccg aaacctaaa aaacccgaa gataacctt 
      cgtcctgaa ggtgaattc gaaagacca agtccaaca 
      aaaacccga aaaacccaa attcaaacc agctgaacg 
      tccccaacc taaaaaacc tgaagacaa tcttcgtcc 
      tgaaggtga attcgaacg gcctgagct aatagaagt 
      gggcccgtg tgaattgcg caaacctat caaacatga 
      agataatct caaacctga aggaacact tgttgttac 
      cagaaagga cgattacaa cgttgtgaa aggcgaacg 
      agttgatgt gatcaaacg tgaagataa tttacgtgt 
      cgaaggaga atgggatat tcggcgaag agacgatta 
      cagtaacac tacagtcgt agatcgggt cgatgttat 
      caaacgcca agacaatct acgtgttga aggagaatg 
      ggatattag acgtagaga tgactacag taatacaac 
      ggtcgtaga tcgcgtcga tgtcatcaa acgccaaga 
      caatttacg cgtcgaggg cgaattcac cgatgtacg 
      aacacgaga tgactacaa agtcgtcaa gggcgaaag 
      atacgatac tgtcattca ccaagacaa tctaaaaac 
      agaaggaga attccgaag tagtcgcac tagcgacga 
      atactccgt tgtcaaagg cgaacgcgc agaaatcat 
      caccagaca agacaatct ccgaatgga aggcgaatt 
      caacgaata ccgaaaacg cgacgaatt caacactcg 
      aattgtcga aaaagctga agtgatccg ccaccaaga 
      caacctgaa actggaagg caccttcga acgcccgtc 
      ccacgtcca cctaggacc tggcgaacg tcgcaaacc 
      aatcaaaca cgaagacaa cctccaccc tgaaggcga 
      cttcgaacg cccagaccg ccccggctt taaaccagc 
      cgaaaggcc gtcaccaat caaacccca agacaatct 
      caaacctga aggaaaatt cgaactacc aacttcccc 
      cggtttcca acccggcga acgtcggac tccgattcg 
      ccaccagga taacttgca cccagaagg cgacttcga 
      acgtcccca aagaccaca ataccaacc aatcgaacg 
      cccaacacc caaaaaacc caaagacaa ccttaaacc 
      cgaaggaac attcgaacg tccaacggc aagtcccgt 
      gggacccgg cgaacggcg aagcccaat ccgccacca 
      tgacaattt aagacctga aggtgcgtt cgaacgccc 
      agacaagcc cggctataa accaggcga acgcccaac 
      tccgaaaaa accccagga taacctacg cccagaagg 
      ggaattcga aaaacgaac cccggcgaa accacaacc 
      tggcgaacg gcgaactcc gatccgcca ccaggataa 
      tctgcaccc tgagggtgc tttcgagcg acccgagaa 
      gcctaaata ctcgcctgg tgaacgcct accaatgaa 
      gaaacccca agataattt gagacccga aggtgagtt 
      tgaaacccc ccagaaacc taaatacgg gcctggcga 
      acgtgcccc catcgtcaa acacccaga caaccttaa 
      acctgaagg cgaattcat cggaacacc caaaagtaa 
      agacgattt taaaccaac tcggggcga acgagctcc 
      ggttaagaa accacaaga caatttgaa aatcacagg 
      agagttcca agacatgac aatgcagaa atcacagtt 
      tgtcgtaac ccgaggtga gagagcaga aatacgcaa 
      acatgaaga ttccctaac tatagggaa cggcaaaac 
      tgaatatat caccacatc gaaggaaac gtttgataa 
      aacaacgcc cacgaaagt ggtcacaag tccagcaac 
      tcctggatc catcaacag gagacgcca cattgagtc 
      acacattac tctagggag tgacacagc agtgatgca 
      aacaacgaa ccaaagcaa ctacaacac ttacaccaa 
      gaaaattga tcgccagtc aacaacagg ccgtaacga 
      agttgcaaa taaaacaac gaacgttgc aacggatag 
      taagacttt ggctgacgg tacaactgt cacaacaac 
      tcgcacaac aaccaaaac aacaggaca acaactggc 
      gaaacaaac ccagcaagt ctccgccga agtgtcaac 
      ccaccaccg caaaaacgt cataacttc ggaggaaat 
      caacaacca gatcctgca caggaaagg gctgcacac 
      ctcgaccga agccctgca tgccacgag ttcggcagc 
      cctagacat gcgaaagtc aatctgcaa tttgcacga 
      ccaaggcca cgtgagcca gaacagcga acggcgaag 
      tctcagctc gatccatcg ctcgacgca ggagagcaa 
      cgccagccg gcgctccca ctttgccac cagttacga 
      gacggtgga aaggccgca gaagatcat ccgcaagga 
      taatttatc agtcggggg gcatttcta cggccagtc 
      ggaggccag gtcgtatgg cgagttcac caagcagca 
      gaatgtgca aaaaattga acgggtgac tcgcaggtc 
      caacgtttc gaatataag tttagggga cagttcttc 
      gacgatcat cacgtcgta taagaagga gtatttgcc 
      gcggaatgt gggcccgtg tccggcggt tttgatcga 
      tttgcccaa aagtcccct gaagcatac gagggacac 
      caagtcgca taaatatta cgcgccggt ttcgagtaa 
      atgatggac tgttaacac gactctaac attgtgata 
      cttgttatt actcataat tttattttt atagaggta 
      ggggacggt ttttatact ttgcttggg acttttgta 
      tcaattaat acgtgccta tgttatttt tgtatgatg 
      caagttgta ttatcacaa agccatcaa taaattaac 
      agtttatta
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