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Gene information
General information
TC009427 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1388675..1389613
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009427
    iBeetle screen iB_01549

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009427 RA
      MPVKYLTPD YSQTLRWND ILTSMYLLL 
      YLSFLYYNN LILSIGHSD SLYNILGLA 
      ALEFIKYSF HVSRTKTSF YKHHAKDFI 
      KNVLVLLLL FATIYVVAV LFGAPILSD 
      FEETCMFSL IVTTFTALP LCLYFGGDN 
      TVHMFLSLA SYDGSDVQK LFMLKLRLT 
      LFGAWLGAI VIPLDWNRP WQDWPIPCS 
      VGAMVGYMV ANFVTSLLQ HQYFAKFIR 
      KTGKYML
    CDS sequence
    • >TC009427 RA
      atgccggtg aaatattta acccccgat tattcgcaa 
      acactgcgc tggaacgac atcctcacc tcaatgtac 
      ttactgcta tacttgagc tttttgtac tacaacaac 
      ctgatttta tcaattgga cactcggac tccctttac 
      aacatccta ggccttgcg gctttggaa tttatcaaa 
      tattcgttt cacgtatct cgcacaaaa accagcttt 
      tataaacac cacgccaaa gactttata aaaaacgtc 
      ctagtcttg ttgttactc tttgccaca atttacgtc 
      gttgctgtt ttgttcgga gcgcctatt ctttccgat 
      tttgaagaa acttgcatg ttttcgttg attgttaca 
      acctttact gcgcttcct ctttgcttg tattttggg 
      ggtgataac acagtacac atgttcttg tcactcgct 
      tcgtatgat ggtagcgat gtccagaag ttgtttatg 
      ctgaaattg aggttgact ctctttggg gcctggctg 
      ggggccatc gtcatacca ctggactgg aacaggccg 
      tggcaggac tggcccatc ccttgcagc gtgggggcc 
      atggtgggc tacatggtc gccaatttt gttacctct 
      ctgctccaa caccagtat ttcgccaag tttatcaga 
      aagactgga aaatatatg ctatga
    mRNA sequence
    • >TC009427 RA
      ataacctaa catattcat tgttcttga aatagttga 
      aattatgtg aattacagc ccaaactgt tgaatttgg 
      gttgaaatg agaactatt attatttaa acagtgcca 
      tgctagtta aacggcgtt aatcgcaaa ttaaatttt 
      tatctcatc tcttgtgac tgtgacgac ggctaggcc 
      caaaatgcc ggtgaaata tttaacccc cgattattc 
      gcaaacact gcgctggaa cgacatcct cacctcaat 
      gtacttact gctatactt gagcttttt gtactacaa 
      caacctgat tttatcaat tggacactc ggactccct 
      ttacaacat cctaggcct tgcggcttt ggaatttat 
      caaatattc gtttcacgt atctcgcac aaaaaccag 
      cttttataa acaccacgc caaagactt tataaaaaa 
      cgtcctagt cttgttgtt actctttgc cacaattta 
      cgtcgttgc tgttttgtt cggagcgcc tattctttc 
      cgattttga agaaacttg catgttttc gttgattgt 
      tacaacctt tactgcgct tcctctttg cttgtattt 
      tgggggtga taacacagt acacatgtt cttgtcact 
      cgcttcgta tgatggtag cgatgtcca gaagttgtt 
      tatgctgaa attgaggtt gactctctt tggggcctg 
      gctgggggc catcgtcat accactgga ctggaacag 
      gccgtggca ggactggcc catcccttg cagcgtggg 
      ggccatggt gggctacat ggtcgccaa ttttgttac 
      ctctctgct ccaacacca gtatttcgc caagtttat 
      cagaaagac tggaaaata tatgctatg agttgtgtt 
      acgcttaaa ataaatgtc gattttcgt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY