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Gene information
General information
TC009389 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1441289..1449884
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009389
    iBeetle screen iB_01544

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    mature egg not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009389 RA
      MGDKVDDTI IKLVRNLKS TVEDEPDFT LGSPYKLSL 
      SSVSNDSQC SSEEFLKVK RPKRESLLN VSYAGSPRE 
      IRRLRTDIL ESRNTILNL ENRIQHMHS VRKEVELMY 
      ENETKLLKK QHEHDRKSI EELEAQLVS IRQREAELK 
      KQLAEVTSN YNMLKVQKD EVIEELEKS LSEMKEESR 
      LFDGEENIE IVALNRKLA EMQMMLDAA EEDADAQKK 
      LVLELEKQL AEKNAIDRE IEKKEQALQ IANLRIKEL 
      EYAKENFLE FQDQAKSQA KKLANYSDM VREVEKLRE 
      ENVRLKDEV KNKLLLEEE VHDLKSRLV KYKEQEKKL 
      ANLEAEKVQ TGIYLDEWR AVARGICET VGSDSSLPH 
      LLRSAVEKL QQQEISLTS TKVELESQL TAALYDAKV 
      AKAEIEKSH KLISELKKT GEQKQALLH RMQKKLSLV 
      SRERDSYRL QLDSYEKDL TMSINPSTI ANSNQLQTQ 
      RERIVALEK IIESYKELN AKLESDLQN TNPALYSDN 
      PNSRAEQLT KLQEEVDQL RMENEMLKQ RRDQLEIQL 
      ENYLVGNDT LQGGQVYHL ANNPLAECL AEREKLVEK 
      LEQEIEKLK RKLKNMSEG IDASKLSES MCPQEVQAL 
      KEQVKTHEV QTQRLKEYF KTSFQEFRN VIYMLLGYK 
      IDRTPTSLY KLTNIYAER AEDQLCFQL NSEGDLNLL 
      ENEFSASLE QMVNLHLRH QKSIPVFLS ALTIDLFNN 
      STMATKTYE IE
    CDS sequence
    • >TC009389 RA
      atgggcgac aaagtagac gacactatc ataaagttg 
      gtgaggaat ttgaagtcg acggtcgag gacgagccc 
      gatttcacc ctgggcagc ccctacaaa ctttctctg 
      agttcggtc tcgaacgac agccaatgc agttccgag 
      gagttcctc aaagtcaag cgaccaaag cgcgaaagt 
      ttgctaaat gtgtcctac gcggggtct cctcgcgag 
      atccgacgt ttgcggacc gatatcttg gagagtcgg 
      aacacgatt ttgaatttg gagaaccgg atccaacac 
      atgcacagt gtgcgcaag gaggtcgag ttaatgtac 
      gagaatgag acaaagttg ttgaagaag caacacgaa 
      catgaccgg aagagtatc gaagagttg gaagcgcag 
      ttggtctca atcaggcaa agggaagcc gagttgaag 
      aaacaactt gctgaggtg accagcaac tacaacatg 
      ctgaaggtg caaaaggac gaagtgatt gaagaactt 
      gagaaaagt ctgtccgag atgaaggaa gagtcgcgg 
      ctttttgac ggtgaagaa aacattgaa attgtggcg 
      ttgaatcgg aagttggcc gagatgcaa atgatgctg 
      gacgccgct gaagaagac gccgacgct cagaaaaaa 
      ttagtgctt gaattagag aaacagttg gcagaaaag 
      aacgccata gacagggaa atcgagaag aaggaacaa 
      gcgctccaa attgccaat ttgcgcata aaagagttg 
      gaatatgcc aaagaaaat tttttggaa ttccaagat 
      caagccaaa agtcaagcg aaaaaattg gctaactac 
      agtgatatg gtcagggaa gttgaaaaa ctgagggaa 
      gaaaacgtc cggttgaaa gacgaagtt aagaacaaa 
      ctattactg gaagaggaa gttcacgat ttgaagtca 
      cgtttggtt aaatataaa gaacaggag aagaaattg 
      gcgaattta gaagctgaa aaagttcaa actggaatt 
      tatttagat gagtggcga gctgtagct cgtggaatt 
      tgcgaaaca gtgggttcg gactcgtct ttgcctcat 
      cttttacgt tcagctgtt gaaaaattg caacaacag 
      gagatttcg ttaacttcg accaaagtt gaattggaa 
      tcgcaatta accgctgca ttatacgac gccaaagtt 
      gcaaaagct gagattgaa aaaagccac aaacttatt 
      tcggagctg aagaaaacc ggtgaacaa aaacaggcg 
      cttttgcac cgtatgcag aaaaaactg tcgttggtc 
      agtcgggag agggacagt tatcggctt cagctcgat 
      tcctacgag aaggattta acaatgtcg ataaatccg 
      tcaacaatt gcaaactcg aatcagctg caaactcag 
      cgggagaga atcgtagcg ttggagaaa atcatcgaa 
      agttacaag gagttgaat gcaaagttg gagtcggat 
      ttgcaaaac actaatcct gcgttgtat tccgataat 
      ccgaacagt agagccgaa caattgacg aaattgcag 
      gaagaagtg gaccagttg cggatggaa aatgagatg 
      ttgaagcaa cgtcgagac caacttgaa attcaattg 
      gaaaactac cttgtaggc aatgatacg ctccaaggg 
      ggccaggtt taccacttg gcgaacaac ccgctagcc 
      gagtgtttg gcagaaagg gagaagttg gtggagaaa 
      ttggagcag gagattgag aaattgaaa cggaagttg 
      aaaaacatg tccgaaggc atcgatgcg agtaagtta 
      agcgagtcg atgtgtccg caagaggtc caagcgttg 
      aaagaacaa gtcaaaacg cacgaagtc caaacgcag 
      cgactcaaa gaatatttc aaaacgtcg tttcaggaa 
      tttcgcaat gttatctac atgttactt ggttataaa 
      atcgatcgg actccgacg tcgttgtat aaactaacg 
      aatatttac gcggaacgg gccgaggat cagttgtgc 
      ttccagttg aatagtgaa ggtgattta aatttgctt 
      gaaaacgag tttagcgca agtcttgag caaatggtc 
      aacttgcat ttaagacac caaaagtcg attcccgtt 
      tttctgagt gccctcact atagatttg ttcaacaat 
      agcacgatg gcaacaaag acttatgag attgagtaa
    mRNA sequence
    • >TC009389 RA
      gttctgtct gcaagcctt tcctcagat aagataaat 
      aaaaatgtc agcaaaccg tcggaaaag tgtcccaag 
      tgtatgggg cctgctcgt agctttcgc gtagaatgt 
      gataccact gaagtgtgg ttttgtgag acaagtaaa 
      agtgaggtt ggtttcgtc gccccttgt caaaatggg 
      cgacaaagt agacgacac tatcataaa gttggtgag 
      gaatttgaa gtcgacggt cgaggacga gcccgattt 
      caccctggg cagccccta caaactttc tctgagttc 
      ggtctcgaa cgacagcca atgcagttc cgaggagtt 
      cctcaaagt caagcgacc aaagcgcga aagtttgct 
      aaatgtgtc ctacgcggg gtctcctcg cgagatccg 
      acgtttgcg gaccgatat cttggagag tcggaacac 
      gattttgaa tttggagaa ccggatcca acacatgca 
      cagtgtgcg caaggaggt cgagttaat gtacgagaa 
      tgagacaaa gttgttgaa gaagcaaca cgaacatga 
      ccggaagag tatcgaaga gttggaagc gcagttggt 
      ctcaatcag gcaaaggga agccgagtt gaagaaaca 
      acttgctga ggtgaccag caactacaa catgctgaa 
      ggtgcaaaa ggacgaagt gattgaaga acttgagaa 
      aagtctgtc cgagatgaa ggaagagtc gcggctttt 
      tgacggtga agaaaacat tgaaattgt ggcgttgaa 
      tcggaagtt ggccgagat gcaaatgat gctggacgc 
      cgctgaaga agacgccga cgctcagaa aaaattagt 
      gcttgaatt agagaaaca gttggcaga aaagaacgc 
      catagacag ggaaatcga gaagaagga acaagcgct 
      ccaaattgc caatttgcg cataaaaga gttggaata 
      tgccaaaga aaatttttt ggaattcca agatcaagc 
      caaaagtca agcgaaaaa attggctaa ctacagtga 
      tatggtcag ggaagttga aaaactgag ggaagaaaa 
      cgtccggtt gaaagacga agttaagaa caaactatt 
      actggaaga ggaagttca cgatttgaa gtcacgttt 
      ggttaaata taaagaaca ggagaagaa attggcgaa 
      tttagaagc tgaaaaagt tcaaactgg aatttattt 
      agatgagtg gcgagctgt agctcgtgg aatttgcga 
      aacagtggg ttcggactc gtctttgcc tcatctttt 
      acgttcagc tgttgaaaa attgcaaca acaggagat 
      ttcgttaac ttcgaccaa agttgaatt ggaatcgca 
      attaaccgc tgcattata cgacgccaa agttgcaaa 
      agctgagat tgaaaaaag ccacaaact tatttcgga 
      gctgaagaa aaccggtga acaaaaaca ggcgctttt 
      gcaccgtat gcagaaaaa actgtcgtt ggtcagtcg 
      ggagaggga cagttatcg gcttcagct cgattccta 
      cgagaagga tttaacaat gtcgataaa tccgtcaac 
      aattgcaaa ctcgaatca gctgcaaac tcagcggga 
      gagaatcgt agcgttgga gaaaatcat cgaaagtta 
      caaggagtt gaatgcaaa gttggagtc ggatttgca 
      aaacactaa tcctgcgtt gtattccga taatccgaa 
      cagtagagc cgaacaatt gacgaaatt gcaggaaga 
      agtggacca gttgcggat ggaaaatga gatgttgaa 
      gcaacgtcg agaccaact tgaaattca attggaaaa 
      ctaccttgt aggcaatga tacgctcca agggggcca 
      ggtttacca cttggcgaa caacccgct agccgagtg 
      tttggcaga aagggagaa gttggtgga gaaattgga 
      gcaggagat tgagaaatt gaaacggaa gttgaaaaa 
      catgtccga aggcatcga tgcgagtaa gttaagcga 
      gtcgatgtg tccgcaaga ggtccaagc gttgaaaga 
      acaagtcaa aacgcacga agtccaaac gcagcgact 
      caaagaata tttcaaaac gtcgtttca ggaatttcg 
      caatgttat ctacatgtt acttggtta taaaatcga 
      tcggactcc gacgtcgtt gtataaact aacgaatat 
      ttacgcgga acgggccga ggatcagtt gtgcttcca 
      gttgaatag tgaaggtga tttaaattt gcttgaaaa 
      cgagtttag cgcaagtct tgagcaaat ggtcaactt 
      gcatttaag acaccaaaa gtcgattcc cgtttttct 
      gagtgccct cactataga tttgttcaa caatagcac 
      gatggcaac aaagactta tgagattga gtaatttaa 
      taccatttt ttaattagt tcgcttagc aaatataca 
      gtttaataa gaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY