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Gene information
General information
TC009382 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1311136..1312133
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009382
    iBeetle screen iB_01543

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    legs shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    motility increased - Number of animals showing the phenotype: 10
    Comment: they climb up the well very fast

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009382 RA
      MINYISRCS FRRFPIPGD SLSRVSSHK FSLLCQCPS 
      YFRFAKLTS NLTIRQENV SRHSSSDSG PPSNKLPPL 
      MEFPKITWP SLIKSIRNF ILATFIIKP YLDRDFNLP 
      DFVVGSKKA VEVVSKKIA EGNVKALDG LVTEDILPA 
      LQRAVTLMS LSQREQIAV EVEDIYFSF PYQIGVMFN 
      EDNDQKRFV EITMVFHAL KGLALMKSR GEEPPLNMG 
      MMPEYQHRI SICNYRFIK EFTKGVDSD WTINLLNHF 
      KPIDEMQE
    CDS sequence
    • >TC009382 RA
      atgataaac tacatttca agatgttca tttcgtcgt 
      ttccccatt cccggtgac tcccttagt cgtgtcagc 
      tcccacaag ttctccttg ctgtgccaa tgcccgtcc 
      tattttcgg tttgcaaag ttaacctca aacttgacg 
      ataagacag gagaatgtt agtcgtcac agttcgtct 
      gacagtggg cctccctct aacaaactg cccccgcta 
      atggaattt ccgaaaata acgtggcct tcattaatt 
      aagtcaatc agaaatttc attttggcc actttcatc 
      ataaagccg tatttggac cgagacttc aacttgcct 
      gatttcgtc gtcggatcg aaaaaagct gtggaggtg 
      gtgtctaaa aagattgca gaagggaac gttaaggct 
      ttggatggg ctagtgaca gaggatatc ttgccggcc 
      ttgcagagg gcagtgact ctcatgtcg ctgtctcaa 
      agggagcaa attgccgtt gaagttgag gacatttat 
      ttcagtttt ccgtaccag ataggagtg atgtttaac 
      gaagataat gatcaaaaa agatttgtg gaaattaca 
      atggtcttt catgcgctt aaaggattg gcgttgatg 
      aagtcacgt ggggaagaa ccgcccctg aatatgggc 
      atgatgccc gaataccag catcgaatt tccatttgt 
      aattatcgg ttcattaag gaattcacc aaaggagtt 
      gatagtgac tggaccatt aatttactg aatcatttt 
      aaaccaatt gatgaaatg caggaataa
    mRNA sequence
    • >TC009382 RA
      gaatgttta aattttttc taagatgat aaactacat 
      ttcaagatg ttcatttcg tcgtttccc cattcccgg 
      tgactccct tagtcgtgt cagctccca caagttctc 
      cttgctgtg ccaatgccc gtcctattt tcggtttgc 
      aaagttaac ctcaaactt gacgataag acaggagaa 
      tgttagtcg tcacagttc gtctgacag tgggcctcc 
      ctctaacaa actgccccc gctaatgga atttccgaa 
      aataacgtg gccttcatt aattaagtc aatcagaaa 
      tttcatttt ggccacttt catcataaa gccgtattt 
      ggaccgaga cttcaactt gcctgattt cgtcgtcgg 
      atcgaaaaa agctgtgga ggtggtgtc taaaaagat 
      tgcagaagg gaacgttaa ggctttgga tgggctagt 
      gacagagga tatcttgcc ggccttgca gagggcagt 
      gactctcat gtcgctgtc tcaaaggga gcaaattgc 
      cgttgaagt tgaggacat ttatttcag ttttccgta 
      ccagatagg agtgatgtt taacgaaga taatgatca 
      aaaaagatt tgtggaaat tacaatggt ctttcatgc 
      gcttaaagg attggcgtt gatgaagtc acgtgggga 
      agaaccgcc cctgaatat gggcatgat gcccgaata 
      ccagcatcg aatttccat ttgtaatta tcggttcat 
      taaggaatt caccaaagg agttgatag tgactggac 
      cattaattt actgaatca ttttaaacc aattgatga 
      aatgcagga ataaataaa caatactta gttaaatgc 
      tcttttttt attaccacc accatgaaa atgtctaaa 
      aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY