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Gene information
General information
TC009381 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1303946..1306167
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009381
    iBeetle screen iB_01542

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    legs podomer random shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs podomer potentially shape fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    tropharium shape irregular - Number of animals showing the phenotype: 4
    previtellogenic egg chamber partiallyfused - Number of animals showing the phenotype: 4
    vitellogenic egg chamber partiallydegenerated - Number of animals showing the phenotype: 4
    mature egg not present - Number of animals showing the phenotype: 4
    egg chamber partiallyanteriorized - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009381 RA
      MDDSEANTF RILLATDLH LGYGLNNSI RENDTFRTF 
      EEILQIANK EKVDFILLG GDLFHEARP TPHCIKKTI 
      ELIRKYCFG DKPVEIEFF SDPSLHFPG NASVNYEDP 
      NINVSIPIF SIHGNHDDP TGKNHVSAL DLFSSMGLV 
      NYFGRWDDV TKVEINPIL LKKGDSKLA LYGLSHIRD 
      ERLARLFLD KKVVTKTPE DLNDWFNVF ILHQNRANR 
      GAKNFIADS FIPEFIDLV MWGHEHDCR IEPSASAGG 
      NCYITQPGS SVATSLAEG EALTKKIGL LRVCGKNFN 
      LHPIELQTV RPFIFSCLS IEPPENFAG KIAHSERTK 
      ILVREKIES MIEEANNLN RDNMLPLIR LIIKYEDER 
      QVFNPIRFG QEFINKVAN PEDIVKFAT HYKQARRTN 
      NVHVNFIDP NDEHVTSVI DLISKYFEG DNHLSALSV 
      TGLNEAVNK FLESNDNDA PQVLLNEQE EYLMKKLME 
      LKPEEAEIE DCLKQISES RSVEEFRDV LRDPNRRQN 
      LNSVTVDDQ ITIDDSDDE NNVSVSSRP AKGPGSRGG 
      RGSRGGRGR GRGRAK
    CDS sequence
    • >TC009381 RA
      atggatgat tccgaagcc aatactttt cgaattttg 
      ctcgcaaca gaccttcat ctaggctac ggccttaat 
      aattcaatc cgagaaaac gacactttc cgcacattt 
      gaggaaatc ctccaaatt gcaaataag gaaaaggtc 
      gattttatt ttactcggg ggcgatttg ttccacgaa 
      gcgcgccct acaccccac tgtatcaaa aaaaccatc 
      gaactgata cgaaagtat tgcttcggg gataaacct 
      gtcgaaatt gagtttttc tccgaccct tcactacac 
      ttccccggc aatgccagc gttaattac gaggacccc 
      aatattaat gtttccatt ccgattttt tcgattcac 
      ggcaatcac gatgacccc actggtaag aaccacgtt 
      tccgccctt gacttgttc tcgtccatg ggtctcgtg 
      aattatttc gggcgctgg gacgacgtc accaaagtc 
      gaaatcaat ccgattttg ctcaaaaag ggggactcg 
      aagttggct ttgtacggc ctgtctcac attcgagac 
      gaacgtttg gctcgctta tttttggac aaaaaagtc 
      gtgactaaa acaccggag gatttgaac gattggttt 
      aacgtgttc attttgcac caaaaccgg gcaaatcga 
      ggggctaaa aacttcata gctgactca ttcatcccc 
      gaatttatc gatctggtg atgtggggg cacgagcac 
      gattgtcgc attgagcca agcgctagt gctgggggc 
      aactgctat ataacgcaa ccggggagc tctgtcgcc 
      acgtctctg gccgagggc gaagccttg acgaaaaaa 
      atcggcctt ttgagggtc tgtggcaag aattttaac 
      ctccatccg attgagttg caaacagtt cggccgttt 
      attttcagc tgtctttcg attgagccc cccgaaaat 
      tttgcaggg aaaatagcg cattctgaa cggactaaa 
      attctagta agggagaaa attgaaagt atgatagaa 
      gaagcgaac aatttgaat agggacaac atgttgccg 
      ctgattagg ttaattatt aaatatgaa gatgagaga 
      caggttttt aatccgatc aggtttgga caagaattt 
      atcaacaaa gtcgctaat ccggaagat atcgtcaag 
      tttgcaacc cattataaa caagcgaga cgaaccaat 
      aatgttcac gtgaatttc atagatccg aatgatgaa 
      cacgttacc agcgttata gacctaatt tcaaaatac 
      ttcgagggg gacaaccac ttatcggct ctgtcggta 
      actggctta aatgaggcc gttaataaa tttctggaa 
      tcgaacgac aacgacgct ccacaagtc ttgttaaat 
      gagcaggag gaatacctc atgaaaaaa ttaatggag 
      cttaagcca gaagaagcc gaaattgaa gattgtttg 
      aaacaaatt agcgagtca cgaagtgtt gaagaattt 
      agggatgtt ttgagggat ccaaaccga cggcaaaat 
      ctaaacagt gtgacagtg gatgatcaa attacgatt 
      gatgatagt gatgatgaa aataatgtc tctgtgtca 
      agtaggccg gctaaaggg cccggttcg aggggcgga 
      cgcgggtcc aggggcggg agaggtcga gggagggga 
      agggccaag tag
    mRNA sequence
    • >TC009381 RA
      agtgaattc ccccgaaac gttagtaaa gaacaattc 
      gtggtagtg tcaccgtgt aaaatggat gattccgaa 
      gccaatact tttcgaatt ttgctcgca acagacctt 
      catctaggc tacggcctt aataattca atccgagaa 
      aacgacact ttccgcaca tttgaggaa atcctccaa 
      attgcaaat aaggaaaag gtcgatttt attttactc 
      gggggcgat ttgttccac gaagcgcgc cctacaccc 
      cactgtatc aaaaaaacc atcgaactg atacgaaag 
      tattgcttc ggggataaa cctgtcgaa attgagttt 
      ttctccgac ccttcacta cacttcccc ggcaatgcc 
      agcgttaat tacgaggac cccaatatt aatgtttcc 
      attccgatt ttttcgatt cacggcaat cacgatgac 
      cccactggt aagaaccac gtttccgcc cttgacttg 
      ttctcgtcc atgggtctc gtgaattat ttcgggcgc 
      tgggacgac gtcaccaaa gtcgaaatc aatccgatt 
      ttgctcaaa aagggggac tcgaagttg gctttgtac 
      ggcctgtct cacattcga gacgaacgt ttggctcgc 
      ttatttttg gacaaaaaa gtcgtgact aaaacaccg 
      gaggatttg aacgattgg tttaacgtg ttcattttg 
      caccaaaac cgggcaaat cgaggggct aaaaacttc 
      atagctgac tcattcatc cccgaattt atcgatctg 
      gtgatgtgg gggcacgag cacgattgt cgcattgag 
      ccaagcgct agtgctggg ggcaactgc tatataacg 
      caaccgggg agctctgtc gccacgtct ctggccgag 
      ggcgaagcc ttgacgaaa aaaatcggc cttttgagg 
      gtctgtggc aagaatttt aacctccat ccgattgag 
      ttgcaaaca gttcggccg tttattttc agctgtctt 
      tcgattgag ccccccgaa aattttgca gggaaaata 
      gcgcattct gaacggact aaaattcta gtaagggag 
      aaaattgaa agtatgata gaagaagcg aacaatttg 
      aatagggac aacatgttg ccgctgatt aggttaatt 
      attaaatat gaagatgag agacaggtt tttaatccg 
      atcaggttt ggacaagaa tttatcaac aaagtcgct 
      aatccggaa gatatcgtc aagtttgca acccattat 
      aaacaagcg agacgaacc aataatgtt cacgtgaat 
      ttcatagat ccgaatgat gaacacgtt accagcgtt 
      atagaccta atttcaaaa tacttcgag ggggacaac 
      cacttatcg gctctgtcg gtaactggc ttaaatgag 
      gccgttaat aaatttctg gaatcgaac gacaacgac 
      gctccacaa gtcttgtta aatgagcag gaggaatac 
      ctcatgaaa aaattaatg gagcttaag ccagaagaa 
      gccgaaatt gaagattgt ttgaaacaa attagcgag 
      tcacgaagt gttgaagaa tttagggat gttttgagg 
      gatccaaac cgacggcaa aatctaaac agtgtgaca 
      gtggatgat caaattacg attgatgat agtgatgat 
      gaaaataat gtctctgtg tcaagtagg ccggctaaa 
      gggcccggt tcgaggggc ggacgcggg tccaggggc 
      gggagaggt cgagggagg ggaagggcc aagtagttg 
      gtaccaaat gatttttac ttttcgttc aaataacga 
      aacatagat gactatttt tgataaaag gtttgtttt 
      ttgttgcat cctagaata tgtgttata cttacctcg 
      tgttgaaac cgtttcatt gcattaaga cgccgtatc 
      gacttttca agagagtaa catacaata ttttttgtg 
      ctgcctgta taaaaattg aatttttta attattaaa 
      ataaatacc acaaaatta t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY