General information | |||
TC009381 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 1303946..1306167 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC009381 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01542 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
remarks: muscle pattern potentially obscured by segmentation defect,
legs podomer random shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs podomer potentially shape fused - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
tropharium shape irregular - Number of animals showing the phenotype: 4
previtellogenic egg chamber partiallyfused - Number of animals showing the phenotype: 4
vitellogenic egg chamber partiallydegenerated - Number of animals showing the phenotype: 4
mature egg not present - Number of animals showing the phenotype: 4
egg chamber partiallyanteriorized - Number of animals showing the phenotype: 4
- >TC009381 RA
MDDSEANTF RILLATDLH LGYGLNNSI RENDTFRTF EEILQIANK EKVDFILLG GDLFHEARP TPHCIKKTI ELIRKYCFG DKPVEIEFF SDPSLHFPG NASVNYEDP NINVSIPIF SIHGNHDDP TGKNHVSAL DLFSSMGLV NYFGRWDDV TKVEINPIL LKKGDSKLA LYGLSHIRD ERLARLFLD KKVVTKTPE DLNDWFNVF ILHQNRANR GAKNFIADS FIPEFIDLV MWGHEHDCR IEPSASAGG NCYITQPGS SVATSLAEG EALTKKIGL LRVCGKNFN LHPIELQTV RPFIFSCLS IEPPENFAG KIAHSERTK ILVREKIES MIEEANNLN RDNMLPLIR LIIKYEDER QVFNPIRFG QEFINKVAN PEDIVKFAT HYKQARRTN NVHVNFIDP NDEHVTSVI DLISKYFEG DNHLSALSV TGLNEAVNK FLESNDNDA PQVLLNEQE EYLMKKLME LKPEEAEIE DCLKQISES RSVEEFRDV LRDPNRRQN LNSVTVDDQ ITIDDSDDE NNVSVSSRP AKGPGSRGG RGSRGGRGR GRGRAK
- >TC009381 RA
atggatgat tccgaagcc aatactttt cgaattttg ctcgcaaca gaccttcat ctaggctac ggccttaat aattcaatc cgagaaaac gacactttc cgcacattt gaggaaatc ctccaaatt gcaaataag gaaaaggtc gattttatt ttactcggg ggcgatttg ttccacgaa gcgcgccct acaccccac tgtatcaaa aaaaccatc gaactgata cgaaagtat tgcttcggg gataaacct gtcgaaatt gagtttttc tccgaccct tcactacac ttccccggc aatgccagc gttaattac gaggacccc aatattaat gtttccatt ccgattttt tcgattcac ggcaatcac gatgacccc actggtaag aaccacgtt tccgccctt gacttgttc tcgtccatg ggtctcgtg aattatttc gggcgctgg gacgacgtc accaaagtc gaaatcaat ccgattttg ctcaaaaag ggggactcg aagttggct ttgtacggc ctgtctcac attcgagac gaacgtttg gctcgctta tttttggac aaaaaagtc gtgactaaa acaccggag gatttgaac gattggttt aacgtgttc attttgcac caaaaccgg gcaaatcga ggggctaaa aacttcata gctgactca ttcatcccc gaatttatc gatctggtg atgtggggg cacgagcac gattgtcgc attgagcca agcgctagt gctgggggc aactgctat ataacgcaa ccggggagc tctgtcgcc acgtctctg gccgagggc gaagccttg acgaaaaaa atcggcctt ttgagggtc tgtggcaag aattttaac ctccatccg attgagttg caaacagtt cggccgttt attttcagc tgtctttcg attgagccc cccgaaaat tttgcaggg aaaatagcg cattctgaa cggactaaa attctagta agggagaaa attgaaagt atgatagaa gaagcgaac aatttgaat agggacaac atgttgccg ctgattagg ttaattatt aaatatgaa gatgagaga caggttttt aatccgatc aggtttgga caagaattt atcaacaaa gtcgctaat ccggaagat atcgtcaag tttgcaacc cattataaa caagcgaga cgaaccaat aatgttcac gtgaatttc atagatccg aatgatgaa cacgttacc agcgttata gacctaatt tcaaaatac ttcgagggg gacaaccac ttatcggct ctgtcggta actggctta aatgaggcc gttaataaa tttctggaa tcgaacgac aacgacgct ccacaagtc ttgttaaat gagcaggag gaatacctc atgaaaaaa ttaatggag cttaagcca gaagaagcc gaaattgaa gattgtttg aaacaaatt agcgagtca cgaagtgtt gaagaattt agggatgtt ttgagggat ccaaaccga cggcaaaat ctaaacagt gtgacagtg gatgatcaa attacgatt gatgatagt gatgatgaa aataatgtc tctgtgtca agtaggccg gctaaaggg cccggttcg aggggcgga cgcgggtcc aggggcggg agaggtcga gggagggga agggccaag tag
- >TC009381 RA
agtgaattc ccccgaaac gttagtaaa gaacaattc gtggtagtg tcaccgtgt aaaatggat gattccgaa gccaatact tttcgaatt ttgctcgca acagacctt catctaggc tacggcctt aataattca atccgagaa aacgacact ttccgcaca tttgaggaa atcctccaa attgcaaat aaggaaaag gtcgatttt attttactc gggggcgat ttgttccac gaagcgcgc cctacaccc cactgtatc aaaaaaacc atcgaactg atacgaaag tattgcttc ggggataaa cctgtcgaa attgagttt ttctccgac ccttcacta cacttcccc ggcaatgcc agcgttaat tacgaggac cccaatatt aatgtttcc attccgatt ttttcgatt cacggcaat cacgatgac cccactggt aagaaccac gtttccgcc cttgacttg ttctcgtcc atgggtctc gtgaattat ttcgggcgc tgggacgac gtcaccaaa gtcgaaatc aatccgatt ttgctcaaa aagggggac tcgaagttg gctttgtac ggcctgtct cacattcga gacgaacgt ttggctcgc ttatttttg gacaaaaaa gtcgtgact aaaacaccg gaggatttg aacgattgg tttaacgtg ttcattttg caccaaaac cgggcaaat cgaggggct aaaaacttc atagctgac tcattcatc cccgaattt atcgatctg gtgatgtgg gggcacgag cacgattgt cgcattgag ccaagcgct agtgctggg ggcaactgc tatataacg caaccgggg agctctgtc gccacgtct ctggccgag ggcgaagcc ttgacgaaa aaaatcggc cttttgagg gtctgtggc aagaatttt aacctccat ccgattgag ttgcaaaca gttcggccg tttattttc agctgtctt tcgattgag ccccccgaa aattttgca gggaaaata gcgcattct gaacggact aaaattcta gtaagggag aaaattgaa agtatgata gaagaagcg aacaatttg aatagggac aacatgttg ccgctgatt aggttaatt attaaatat gaagatgag agacaggtt tttaatccg atcaggttt ggacaagaa tttatcaac aaagtcgct aatccggaa gatatcgtc aagtttgca acccattat aaacaagcg agacgaacc aataatgtt cacgtgaat ttcatagat ccgaatgat gaacacgtt accagcgtt atagaccta atttcaaaa tacttcgag ggggacaac cacttatcg gctctgtcg gtaactggc ttaaatgag gccgttaat aaatttctg gaatcgaac gacaacgac gctccacaa gtcttgtta aatgagcag gaggaatac ctcatgaaa aaattaatg gagcttaag ccagaagaa gccgaaatt gaagattgt ttgaaacaa attagcgag tcacgaagt gttgaagaa tttagggat gttttgagg gatccaaac cgacggcaa aatctaaac agtgtgaca gtggatgat caaattacg attgatgat agtgatgat gaaaataat gtctctgtg tcaagtagg ccggctaaa gggcccggt tcgaggggc ggacgcggg tccaggggc gggagaggt cgagggagg ggaagggcc aagtagttg gtaccaaat gatttttac ttttcgttc aaataacga aacatagat gactatttt tgataaaag gtttgtttt ttgttgcat cctagaata tgtgttata cttacctcg tgttgaaac cgtttcatt gcattaaga cgccgtatc gacttttca agagagtaa catacaata ttttttgtg ctgcctgta taaaaattg aatttttta attattaaa ataaatacc acaaaatta t
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
