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Gene information
General information
TC009371 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid46: 1016496..1027068
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009371
    iBeetle screen iB_01540
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009371 RA
      MSESNPKLD SGTRLPLHH RNPSYTIRV ICCVLFIFI 
      TEICLAHYI YRLINSEVR SDYVSKEDF RQFFLNELR 
      ADQAKDEIV RILILQEHQ KGTRRRRDA DDYQMLNSP 
      SDEPHVEFF SPEMRSTLE KKDELIRQQ TGNKGAAPG 
      GDSWIWLTS YSRIPEKAL EGYCKKVHQ FCPPGRPGP 
      TGPPGSQGP KGDRGDKGF PGVPGQMGH RGLPGLPGE 
      KGSQGRPGL DGRDGIPGE PGLDGMPGR NGYDGIPGV 
      NGRPGLPGR DGRNGTDGL PGLPGPQGP PGPPGPKGL 
      TGPKGKPGR HGRDGDPGK PGGTFYQIN GKNVSELLI 
      PPSIAGAQA TPMGPIVVH EGENVRLHC SAIGTPNPH 
      VTWMKLGKR PVSRGAWHD TGVSGHTLN ITRVNRVHM 
      GQYKCIADN GIPPQASQT FNLEVYFPP LIRIYNQVV 
      EVPTGSSAV LECETEAFP ESIRYWERS DGRLLENGN 
      KYQIDNSLD RDLYKARMQ LNITRVHSN DMTYKYYCV 
      SKNELKTIR GELQIQEQQ PGRPRQQLN DKKTSIFGN 
      LPPIQVSEE DLCGPPVQC PECEACSAS GVSLIDLIS 
      RWNIRKFGN IMYDKDYAN RTTNCVLYA VGKPVYHRY 
      SDHNNGCWM RDSNPLVEK DAEKFWVTQ EDDPSHLFQ 
      FDNKTMFRR NAPSRTYVL SYPFQGNAH VIYNGSFYY 
      NAKASNKII KFNLQTSHS DNLSLPFPY PLNSSNLYT 
      AQYNYMDFS VDDNGLWVI FPVPDSNNT AVLKMDVNL 
      MKINYIWNV SINHNKVGE MFIVCGVLY AVDSVTDRN 
      TKIRFALDL YNEKLLDVN LAFSNPFRK TTMIGYNHR 
      NKELYTWDK GNQLTYPVR YHESGYNMT DRDDKIFAS 
      EFAQRDMPT GYNVFH
    CDS sequence
    • >TC009371 RA
      atgtcggaa tcaaacccc aaactcgac tccggcacc 
      cgtttgccg ctccaccac cgcaacccc agctacaca 
      atccgcgtg atctgctgc gtcctcttc atcttcatc 
      accgagatc tgcctggcg cactacatc taccgactg 
      atcaactcg gaagtccgc agcgactac gtctccaaa 
      gaggacttc cgccagttc ttcctcaac gagctgcgc 
      gccgaccag gccaaggac gaaatcgtg cggattctc 
      atcctgcag gagcaccag aagggcacc aggaggcga 
      cgcgatgcc gacgattac caaatgctc aactcgccg 
      agcgacgag ccgcatgtg gagttcttc agtccggag 
      atgaggagc acgctggag aagaaggac gagttgata 
      aggcagcag acggggaac aagggggcg gctcctgga 
      ggcgattcc tggatctgg ctcacgagc tacagccgg 
      attccggaa aaggcattg gagggctac tgcaagaag 
      gtgcaccag ttttgccct ccaggtcgg cctggtccc 
      acaggacct ccgggaagt caaggacct aaaggcgac 
      cgcggagac aagggtttt cctggcgtg ccgggccaa 
      atgggtcac cgaggttta ccaggcctt ccgggagaa 
      aaaggatcg caaggtcgt cctggattg gatggtcgg 
      gatggtatt ccgggtgag ccaggcctg gatggaatg 
      cctggacgc aatggttac gacggtatt cctggggtt 
      aacggtaga cctgggtta ccggggaga gacgggcgc 
      aatggaacc gatggactt cccggcttg ccaggccct 
      caaggccct ccaggcccc ccaggccct aagggactt 
      acgggccct aaagggaag cccggccga cacggccgt 
      gatggagat ccgggaaag ccaggcggg acgttttat 
      caaatcaac ggaaaaaac gtctccgag ttgctgata 
      ccgccctcg attgcaggg gctcaagcc actccgatg 
      ggacccatc gtggtacac gagggagaa aatgttcga 
      ttgcactgc tcggcgatc ggcactccc aacccccac 
      gtgacctgg atgaagctg gggaagcgg cccgtcagc 
      aggggggcc tggcatgat acgggagtt tcaggccac 
      acgttgaac ataacgagg gttaaccga gtgcacatg 
      ggccagtac aagtgcatt gctgataac ggcattccg 
      cctcaagcg agccaaact tttaatttg gaagtctat 
      tttccgccc cttatccgg atatacaat caggtggtt 
      gaagtgccg actggatct tcggccgtt cttgaatgc 
      gaaaccgag gcgttcccg gaatctatt agatactgg 
      gagagatcc gatggacgt ctcttggaa aacggaaat 
      aaatatcaa attgacaac tcgctagac agggatctc 
      tacaaagcg agaatgcag ctgaacata accagagta 
      cattcaaac gacatgact tataaatat tactgcgtc 
      agtaagaac gagctgaaa acaatcaga ggagaactc 
      cagattcaa gaacaacag ccaggcaga ccgcgccag 
      caacttaac gacaagaaa accagcatt tttggcaat 
      ttgcccccc attcaagtc tcggaggaa gacctgtgt 
      ggcccccct gtccagtgt ccggagtgt gaagcttgc 
      agtgcgagc ggtgtctcg ctaatcgac ctcataagt 
      cgctggaac atcaggaaa ttcggcaac ataatgtac 
      gacaaggac tacgcaaac agaaccaca aattgcgtt 
      ttatacgcg gtgggcaaa cccgtgtac caccgctat 
      tcggaccac aacaatggg tgttggatg cgcgattcg 
      aacccattg gttgaaaaa gacgccgaa aagttctgg 
      gtgacccag gaggacgac ccaagccac ctgttccag 
      ttcgataac aagaccatg tttcggcga aacgcccca 
      tccagaacc tatgtgctg agttatccg tttcagggg 
      aacgcacac gtcatttat aacggatcg ttctattac 
      aacgctaaa gcgtccaac aaaataatc aaatttaat 
      ctgcagacg agccactcg gataatttg agcttgccc 
      tttccttac ccccttaat tcgtcgaat ctctacaca 
      gcgcagtac aactacatg gatttcagc gttgacgac 
      aacggattg tgggttatt tttcccgtt ccggattcg 
      aacaacacg gcggttttg aagatggat gtaaacttg 
      atgaaaatc aactacatc tggaacgta agcataaac 
      cacaataag gtgggtgaa atgttcatc gtttgtgga 
      gtgctatac gcagtggac agcgtaaca gacaggaac 
      acgaaaatt cgattcgcc ctcgatctg tacaacgag 
      aagcttctc gatgttaat cttgcattt tcgaaccct 
      tttaggaaa acaacaatg ataggatac aaccaccgg 
      aataaggag ctgtatacg tgggataag ggcaatcaa 
      ttgacttat cccgtgcgg tatcacgag tcgggttat 
      aacatgacg gacagggac gataaaatt ttcgcaagc 
      gaatttgcg caaagggat atgcctaca gggtacaac 
      gttttccat tga
    mRNA sequence
    • >TC009371 RA
      ccgctgcag gaagaagct cgcttgcgt ttcctcctc 
      gtccgcgat gtgatttcc ggaatgtcg gaatcaaac 
      cccaaactc gactccggc acccgtttg ccgctccac 
      caccgcaac cccagctac acaatccgc gtgatctgc 
      tgcgtcctc ttcatcttc atcaccgag atctgcctg 
      gcgcactac atctaccga ctgatcaac tcggaagtc 
      cgcagcgac tacgtctcc aaagaggac ttccgccag 
      ttcttcctc aacgagctg cgcgccgac caggccaag 
      gacgaaatc gtgcggatt ctcatcctg caggagcac 
      cagaagggc accaggagg cgacgcgat gccgacgat 
      taccaaatg ctcaactcg ccgagcgac gagccgcat 
      gtggagttc ttcagtccg gagatgagg agcacgctg 
      gagaagaag gacgagttg ataaggcag cagacgggg 
      aacaagggg gcggctcct ggaggcgat tcctggatc 
      tggctcacg agctacagc cggattccg gaaaaggca 
      ttggagggc tactgcaag aaggtgcac cagttttgc 
      cctccaggt cggcctggt cccacagga cctccggga 
      agtcaagga cctaaaggc gaccgcgga gacaagggt 
      tttcctggc gtgccgggc caaatgggt caccgaggt 
      ttaccaggc cttccggga gaaaaagga tcgcaaggt 
      cgtcctgga ttggatggt cgggatggt attccgggt 
      gagccaggc ctggatgga atgcctgga cgcaatggt 
      tacgacggt attcctggg gttaacggt agacctggg 
      ttaccgggg agagacggg cgcaatgga accgatgga 
      cttcccggc ttgccaggc cctcaaggc cctccaggc 
      cccccaggc cctaaggga cttacgggc cctaaaggg 
      aagcccggc cgacacggc cgtgatgga gatccggga 
      aagccaggc gggacgttt tatcaaatc aacggaaaa 
      aacgtctcc gagttgctg ataccgccc tcgattgca 
      ggggctcaa gccactccg atgggaccc atcgtggta 
      cacgaggga gaaaatgtt cgattgcac tgctcggcg 
      atcggcact cccaacccc cacgtgacc tggatgaag 
      ctggggaag cggcccgtc agcaggggg gcctggcat 
      gatacggga gtttcaggc cacacgttg aacataacg 
      agggttaac cgagtgcac atgggccag tacaagtgc 
      attgctgat aacggcatt ccgcctcaa gcgagccaa 
      acttttaat ttggaagtc tattttccg ccccttatc 
      cggatatac aatcaggtg gttgaagtg ccgactgga 
      tcttcggcc gttcttgaa tgcgaaacc gaggcgttc 
      ccggaatct attagatac tgggagaga tccgatgga 
      cgtctcttg gaaaacgga aataaatat caaattgac 
      aactcgcta gacagggat ctctacaaa gcgagaatg 
      cagctgaac ataaccaga gtacattca aacgacatg 
      acttataaa tattactgc gtcagtaag aacgagctg 
      aaaacaatc agaggagaa ctccagatt caagaacaa 
      cagccaggc agaccgcgc cagcaactt aacgacaag 
      aaaaccagc atttttggc aatttgccc cccattcaa 
      gtctcggag gaagacctg tgtggcccc cctgtccag 
      tgtccggag tgtgaagct tgcagtgcg agcggtgtc 
      tcgctaatc gacctcata agtcgctgg aacatcagg 
      aaattcggc aacataatg tacgacaag gactacgca 
      aacagaacc acaaattgc gttttatac gcggtgggc 
      aaacccgtg taccaccgc tattcggac cacaacaat 
      gggtgttgg atgcgcgat tcgaaccca ttggttgaa 
      aaagacgcc gaaaagttc tgggtgacc caggaggac 
      gacccaagc cacctgttc cagttcgat aacaagacc 
      atgtttcgg cgaaacgcc ccatccaga acctatgtg 
      ctgagttat ccgtttcag gggaacgca cacgtcatt 
      tataacgga tcgttctat tacaacgct aaagcgtcc 
      aacaaaata atcaaattt aatctgcag acgagccac 
      tcggataat ttgagcttg ccctttcct taccccctt 
      aattcgtcg aatctctac acagcgcag tacaactac 
      atggatttc agcgttgac gacaacgga ttgtgggtt 
      atttttccc gttccggat tcgaacaac acggcggtt 
      ttgaagatg gatgtaaac ttgatgaaa atcaactac 
      atctggaac gtaagcata aaccacaat aaggtgggt 
      gaaatgttc atcgtttgt ggagtgcta tacgcagtg 
      gacagcgta acagacagg aacacgaaa attcgattc 
      gccctcgat ctgtacaac gagaagctt ctcgatgtt 
      aatcttgca ttttcgaac ccttttagg aaaacaaca 
      atgatagga tacaaccac cggaataag gagctgtat 
      acgtgggat aagggcaat caattgact tatcccgtg 
      cggtatcac gagtcgggt tataacatg acggacagg 
      gacgataaa attttcgca agcgaattt gcgcaaagg 
      gatatgcct acagggtac aacgttttc cattgaggg 
      acgacttct tacagtaga gtagatctc cgttttaga 
      taatttgta ctaattgta atcaatggt gtttgtggc 
      ccttgtaaa taaaaactg tttttttct