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Gene information
General information
TC009371 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1136697..1147269
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009371
    iBeetle screen iB_01540

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009371 RA
      MSESNPKLD SGTRLPLHH RNPSYTIRV ICCVLFIFI 
TEICLAHYI YRLINSEVR SDYVSKEDF RQFFLNELR 
ADQAKDEIV RILILQEHQ KGTRRRRDA DDYQMLNSP 
SDEPHVEFF SPEMRSTLE KKDELIRQQ TGNKGAAPG 
GDSWIWLTS YSRIPEKAL EGYCKKVHQ FCPPGRPGP 
TGPPGSQGP KGDRGDKGF PGVPGQMGH RGLPGLPGE 
KGSQGRPGL DGRDGIPGE PGLDGMPGR NGYDGIPGV 
NGRPGLPGR DGRNGTDGL PGLPGPQGP PGPPGPKGL 
TGPKGKPGR HGRDGDPGK PGGTFYQIN GKNVSELLI 
PPSIAGAQA TPMGPIVVH EGENVRLHC SAIGTPNPH 
VTWMKLGKR PVSRGAWHD TGVSGHTLN ITRVNRVHM 
GQYKCIADN GIPPQASQT FNLEVYFPP LIRIYNQVV 
EVPTGSSAV LECETEAFP ESIRYWERS DGRLLENGN 
KYQIDNSLD RDLYKARMQ LNITRVHSN DMTYKYYCV 
SKNELKTIR GELQIQEQQ PGRPRQQLN DKKTSIFGN 
LPPIQVSEE DLCGPPVQC PECEACSAS GVSLIDLIS 
RWNIRKFGN IMYDKDYAN RTTNCVLYA VGKPVYHRY 
SDHNNGCWM RDSNPLVEK DAEKFWVTQ EDDPSHLFQ 
FDNKTMFRR NAPSRTYVL SYPFQGNAH VIYNGSFYY 
NAKASNKII KFNLQTSHS DNLSLPFPY PLNSSNLYT 
AQYNYMDFS VDDNGLWVI FPVPDSNNT AVLKMDVNL 
MKINYIWNV SINHNKVGE MFIVCGVLY AVDSVTDRN 
TKIRFALDL YNEKLLDVN LAFSNPFRK TTMIGYNHR 
NKELYTWDK GNQLTYPVR YHESGYNMT DRDDKIFAS 
EFAQRDMPT GYNVFH
    CDS sequence
    • >TC009371 RA
      atgtcggaa tcaaacccc aaactcgac tccggcacc 
cgtttgccg ctccaccac cgcaacccc agctacaca 
atccgcgtg atctgctgc gtcctcttc atcttcatc 
accgagatc tgcctggcg cactacatc taccgactg 
atcaactcg gaagtccgc agcgactac gtctccaaa 
gaggacttc cgccagttc ttcctcaac gagctgcgc 
gccgaccag gccaaggac gaaatcgtg cggattctc 
atcctgcag gagcaccag aagggcacc aggaggcga 
cgcgatgcc gacgattac caaatgctc aactcgccg 
agcgacgag ccgcatgtg gagttcttc agtccggag 
atgaggagc acgctggag aagaaggac gagttgata 
aggcagcag acggggaac aagggggcg gctcctgga 
ggcgattcc tggatctgg ctcacgagc tacagccgg 
attccggaa aaggcattg gagggctac tgcaagaag 
gtgcaccag ttttgccct ccaggtcgg cctggtccc 
acaggacct ccgggaagt caaggacct aaaggcgac 
cgcggagac aagggtttt cctggcgtg ccgggccaa 
atgggtcac cgaggttta ccaggcctt ccgggagaa 
aaaggatcg caaggtcgt cctggattg gatggtcgg 
gatggtatt ccgggtgag ccaggcctg gatggaatg 
cctggacgc aatggttac gacggtatt cctggggtt 
aacggtaga cctgggtta ccggggaga gacgggcgc 
aatggaacc gatggactt cccggcttg ccaggccct 
caaggccct ccaggcccc ccaggccct aagggactt 
acgggccct aaagggaag cccggccga cacggccgt 
gatggagat ccgggaaag ccaggcggg acgttttat 
caaatcaac ggaaaaaac gtctccgag ttgctgata 
ccgccctcg attgcaggg gctcaagcc actccgatg 
ggacccatc gtggtacac gagggagaa aatgttcga 
ttgcactgc tcggcgatc ggcactccc aacccccac 
gtgacctgg atgaagctg gggaagcgg cccgtcagc 
aggggggcc tggcatgat acgggagtt tcaggccac 
acgttgaac ataacgagg gttaaccga gtgcacatg 
ggccagtac aagtgcatt gctgataac ggcattccg 
cctcaagcg agccaaact tttaatttg gaagtctat 
tttccgccc cttatccgg atatacaat caggtggtt 
gaagtgccg actggatct tcggccgtt cttgaatgc 
gaaaccgag gcgttcccg gaatctatt agatactgg 
gagagatcc gatggacgt ctcttggaa aacggaaat 
aaatatcaa attgacaac tcgctagac agggatctc 
tacaaagcg agaatgcag ctgaacata accagagta 
cattcaaac gacatgact tataaatat tactgcgtc 
agtaagaac gagctgaaa acaatcaga ggagaactc 
cagattcaa gaacaacag ccaggcaga ccgcgccag 
caacttaac gacaagaaa accagcatt tttggcaat 
ttgcccccc attcaagtc tcggaggaa gacctgtgt 
ggcccccct gtccagtgt ccggagtgt gaagcttgc 
agtgcgagc ggtgtctcg ctaatcgac ctcataagt 
cgctggaac atcaggaaa ttcggcaac ataatgtac 
gacaaggac tacgcaaac agaaccaca aattgcgtt 
ttatacgcg gtgggcaaa cccgtgtac caccgctat 
tcggaccac aacaatggg tgttggatg cgcgattcg 
aacccattg gttgaaaaa gacgccgaa aagttctgg 
gtgacccag gaggacgac ccaagccac ctgttccag 
ttcgataac aagaccatg tttcggcga aacgcccca 
tccagaacc tatgtgctg agttatccg tttcagggg 
aacgcacac gtcatttat aacggatcg ttctattac 
aacgctaaa gcgtccaac aaaataatc aaatttaat 
ctgcagacg agccactcg gataatttg agcttgccc 
tttccttac ccccttaat tcgtcgaat ctctacaca 
gcgcagtac aactacatg gatttcagc gttgacgac 
aacggattg tgggttatt tttcccgtt ccggattcg 
aacaacacg gcggttttg aagatggat gtaaacttg 
atgaaaatc aactacatc tggaacgta agcataaac 
cacaataag gtgggtgaa atgttcatc gtttgtgga 
gtgctatac gcagtggac agcgtaaca gacaggaac 
acgaaaatt cgattcgcc ctcgatctg tacaacgag 
aagcttctc gatgttaat cttgcattt tcgaaccct 
tttaggaaa acaacaatg ataggatac aaccaccgg 
aataaggag ctgtatacg tgggataag ggcaatcaa 
ttgacttat cccgtgcgg tatcacgag tcgggttat 
aacatgacg gacagggac gataaaatt ttcgcaagc 
gaatttgcg caaagggat atgcctaca gggtacaac 
gttttccat tga
    mRNA sequence
    • >TC009371 RA
      ccgctgcag gaagaagct cgcttgcgt ttcctcctc 
gtccgcgat gtgatttcc ggaatgtcg gaatcaaac 
cccaaactc gactccggc acccgtttg ccgctccac 
caccgcaac cccagctac acaatccgc gtgatctgc 
tgcgtcctc ttcatcttc atcaccgag atctgcctg 
gcgcactac atctaccga ctgatcaac tcggaagtc 
cgcagcgac tacgtctcc aaagaggac ttccgccag 
ttcttcctc aacgagctg cgcgccgac caggccaag 
gacgaaatc gtgcggatt ctcatcctg caggagcac 
cagaagggc accaggagg cgacgcgat gccgacgat 
taccaaatg ctcaactcg ccgagcgac gagccgcat 
gtggagttc ttcagtccg gagatgagg agcacgctg 
gagaagaag gacgagttg ataaggcag cagacgggg 
aacaagggg gcggctcct ggaggcgat tcctggatc 
tggctcacg agctacagc cggattccg gaaaaggca 
ttggagggc tactgcaag aaggtgcac cagttttgc 
cctccaggt cggcctggt cccacagga cctccggga 
agtcaagga cctaaaggc gaccgcgga gacaagggt 
tttcctggc gtgccgggc caaatgggt caccgaggt 
ttaccaggc cttccggga gaaaaagga tcgcaaggt 
cgtcctgga ttggatggt cgggatggt attccgggt 
gagccaggc ctggatgga atgcctgga cgcaatggt 
tacgacggt attcctggg gttaacggt agacctggg 
ttaccgggg agagacggg cgcaatgga accgatgga 
cttcccggc ttgccaggc cctcaaggc cctccaggc 
cccccaggc cctaaggga cttacgggc cctaaaggg 
aagcccggc cgacacggc cgtgatgga gatccggga 
aagccaggc gggacgttt tatcaaatc aacggaaaa 
aacgtctcc gagttgctg ataccgccc tcgattgca 
ggggctcaa gccactccg atgggaccc atcgtggta 
cacgaggga gaaaatgtt cgattgcac tgctcggcg 
atcggcact cccaacccc cacgtgacc tggatgaag 
ctggggaag cggcccgtc agcaggggg gcctggcat 
gatacggga gtttcaggc cacacgttg aacataacg 
agggttaac cgagtgcac atgggccag tacaagtgc 
attgctgat aacggcatt ccgcctcaa gcgagccaa 
acttttaat ttggaagtc tattttccg ccccttatc 
cggatatac aatcaggtg gttgaagtg ccgactgga 
tcttcggcc gttcttgaa tgcgaaacc gaggcgttc 
ccggaatct attagatac tgggagaga tccgatgga 
cgtctcttg gaaaacgga aataaatat caaattgac 
aactcgcta gacagggat ctctacaaa gcgagaatg 
cagctgaac ataaccaga gtacattca aacgacatg 
acttataaa tattactgc gtcagtaag aacgagctg 
aaaacaatc agaggagaa ctccagatt caagaacaa 
cagccaggc agaccgcgc cagcaactt aacgacaag 
aaaaccagc atttttggc aatttgccc cccattcaa 
gtctcggag gaagacctg tgtggcccc cctgtccag 
tgtccggag tgtgaagct tgcagtgcg agcggtgtc 
tcgctaatc gacctcata agtcgctgg aacatcagg 
aaattcggc aacataatg tacgacaag gactacgca 
aacagaacc acaaattgc gttttatac gcggtgggc 
aaacccgtg taccaccgc tattcggac cacaacaat 
gggtgttgg atgcgcgat tcgaaccca ttggttgaa 
aaagacgcc gaaaagttc tgggtgacc caggaggac 
gacccaagc cacctgttc cagttcgat aacaagacc 
atgtttcgg cgaaacgcc ccatccaga acctatgtg 
ctgagttat ccgtttcag gggaacgca cacgtcatt 
tataacgga tcgttctat tacaacgct aaagcgtcc 
aacaaaata atcaaattt aatctgcag acgagccac 
tcggataat ttgagcttg ccctttcct taccccctt 
aattcgtcg aatctctac acagcgcag tacaactac 
atggatttc agcgttgac gacaacgga ttgtgggtt 
atttttccc gttccggat tcgaacaac acggcggtt 
ttgaagatg gatgtaaac ttgatgaaa atcaactac 
atctggaac gtaagcata aaccacaat aaggtgggt 
gaaatgttc atcgtttgt ggagtgcta tacgcagtg 
gacagcgta acagacagg aacacgaaa attcgattc 
gccctcgat ctgtacaac gagaagctt ctcgatgtt 
aatcttgca ttttcgaac ccttttagg aaaacaaca 
atgatagga tacaaccac cggaataag gagctgtat 
acgtgggat aagggcaat caattgact tatcccgtg 
cggtatcac gagtcgggt tataacatg acggacagg 
gacgataaa attttcgca agcgaattt gcgcaaagg 
gatatgcct acagggtac aacgttttc cattgaggg 
acgacttct tacagtaga gtagatctc cgttttaga 
taatttgta ctaattgta atcaatggt gtttgtggc 
ccttgtaaa taaaaactg tttttttct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY