| General information | |||
| TC009371 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 1136697..1147269 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009371 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01540 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC009371 RA
MSESNPKLD SGTRLPLHH RNPSYTIRV ICCVLFIFI TEICLAHYI YRLINSEVR SDYVSKEDF RQFFLNELR ADQAKDEIV RILILQEHQ KGTRRRRDA DDYQMLNSP SDEPHVEFF SPEMRSTLE KKDELIRQQ TGNKGAAPG GDSWIWLTS YSRIPEKAL EGYCKKVHQ FCPPGRPGP TGPPGSQGP KGDRGDKGF PGVPGQMGH RGLPGLPGE KGSQGRPGL DGRDGIPGE PGLDGMPGR NGYDGIPGV NGRPGLPGR DGRNGTDGL PGLPGPQGP PGPPGPKGL TGPKGKPGR HGRDGDPGK PGGTFYQIN GKNVSELLI PPSIAGAQA TPMGPIVVH EGENVRLHC SAIGTPNPH VTWMKLGKR PVSRGAWHD TGVSGHTLN ITRVNRVHM GQYKCIADN GIPPQASQT FNLEVYFPP LIRIYNQVV EVPTGSSAV LECETEAFP ESIRYWERS DGRLLENGN KYQIDNSLD RDLYKARMQ LNITRVHSN DMTYKYYCV SKNELKTIR GELQIQEQQ PGRPRQQLN DKKTSIFGN LPPIQVSEE DLCGPPVQC PECEACSAS GVSLIDLIS RWNIRKFGN IMYDKDYAN RTTNCVLYA VGKPVYHRY SDHNNGCWM RDSNPLVEK DAEKFWVTQ EDDPSHLFQ FDNKTMFRR NAPSRTYVL SYPFQGNAH VIYNGSFYY NAKASNKII KFNLQTSHS DNLSLPFPY PLNSSNLYT AQYNYMDFS VDDNGLWVI FPVPDSNNT AVLKMDVNL MKINYIWNV SINHNKVGE MFIVCGVLY AVDSVTDRN TKIRFALDL YNEKLLDVN LAFSNPFRK TTMIGYNHR NKELYTWDK GNQLTYPVR YHESGYNMT DRDDKIFAS EFAQRDMPT GYNVFH
- >TC009371 RA
atgtcggaa tcaaacccc aaactcgac tccggcacc cgtttgccg ctccaccac cgcaacccc agctacaca atccgcgtg atctgctgc gtcctcttc atcttcatc accgagatc tgcctggcg cactacatc taccgactg atcaactcg gaagtccgc agcgactac gtctccaaa gaggacttc cgccagttc ttcctcaac gagctgcgc gccgaccag gccaaggac gaaatcgtg cggattctc atcctgcag gagcaccag aagggcacc aggaggcga cgcgatgcc gacgattac caaatgctc aactcgccg agcgacgag ccgcatgtg gagttcttc agtccggag atgaggagc acgctggag aagaaggac gagttgata aggcagcag acggggaac aagggggcg gctcctgga ggcgattcc tggatctgg ctcacgagc tacagccgg attccggaa aaggcattg gagggctac tgcaagaag gtgcaccag ttttgccct ccaggtcgg cctggtccc acaggacct ccgggaagt caaggacct aaaggcgac cgcggagac aagggtttt cctggcgtg ccgggccaa atgggtcac cgaggttta ccaggcctt ccgggagaa aaaggatcg caaggtcgt cctggattg gatggtcgg gatggtatt ccgggtgag ccaggcctg gatggaatg cctggacgc aatggttac gacggtatt cctggggtt aacggtaga cctgggtta ccggggaga gacgggcgc aatggaacc gatggactt cccggcttg ccaggccct caaggccct ccaggcccc ccaggccct aagggactt acgggccct aaagggaag cccggccga cacggccgt gatggagat ccgggaaag ccaggcggg acgttttat caaatcaac ggaaaaaac gtctccgag ttgctgata ccgccctcg attgcaggg gctcaagcc actccgatg ggacccatc gtggtacac gagggagaa aatgttcga ttgcactgc tcggcgatc ggcactccc aacccccac gtgacctgg atgaagctg gggaagcgg cccgtcagc aggggggcc tggcatgat acgggagtt tcaggccac acgttgaac ataacgagg gttaaccga gtgcacatg ggccagtac aagtgcatt gctgataac ggcattccg cctcaagcg agccaaact tttaatttg gaagtctat tttccgccc cttatccgg atatacaat caggtggtt gaagtgccg actggatct tcggccgtt cttgaatgc gaaaccgag gcgttcccg gaatctatt agatactgg gagagatcc gatggacgt ctcttggaa aacggaaat aaatatcaa attgacaac tcgctagac agggatctc tacaaagcg agaatgcag ctgaacata accagagta cattcaaac gacatgact tataaatat tactgcgtc agtaagaac gagctgaaa acaatcaga ggagaactc cagattcaa gaacaacag ccaggcaga ccgcgccag caacttaac gacaagaaa accagcatt tttggcaat ttgcccccc attcaagtc tcggaggaa gacctgtgt ggcccccct gtccagtgt ccggagtgt gaagcttgc agtgcgagc ggtgtctcg ctaatcgac ctcataagt cgctggaac atcaggaaa ttcggcaac ataatgtac gacaaggac tacgcaaac agaaccaca aattgcgtt ttatacgcg gtgggcaaa cccgtgtac caccgctat tcggaccac aacaatggg tgttggatg cgcgattcg aacccattg gttgaaaaa gacgccgaa aagttctgg gtgacccag gaggacgac ccaagccac ctgttccag ttcgataac aagaccatg tttcggcga aacgcccca tccagaacc tatgtgctg agttatccg tttcagggg aacgcacac gtcatttat aacggatcg ttctattac aacgctaaa gcgtccaac aaaataatc aaatttaat ctgcagacg agccactcg gataatttg agcttgccc tttccttac ccccttaat tcgtcgaat ctctacaca gcgcagtac aactacatg gatttcagc gttgacgac aacggattg tgggttatt tttcccgtt ccggattcg aacaacacg gcggttttg aagatggat gtaaacttg atgaaaatc aactacatc tggaacgta agcataaac cacaataag gtgggtgaa atgttcatc gtttgtgga gtgctatac gcagtggac agcgtaaca gacaggaac acgaaaatt cgattcgcc ctcgatctg tacaacgag aagcttctc gatgttaat cttgcattt tcgaaccct tttaggaaa acaacaatg ataggatac aaccaccgg aataaggag ctgtatacg tgggataag ggcaatcaa ttgacttat cccgtgcgg tatcacgag tcgggttat aacatgacg gacagggac gataaaatt ttcgcaagc gaatttgcg caaagggat atgcctaca gggtacaac gttttccat tga
- >TC009371 RA
ccgctgcag gaagaagct cgcttgcgt ttcctcctc gtccgcgat gtgatttcc ggaatgtcg gaatcaaac cccaaactc gactccggc acccgtttg ccgctccac caccgcaac cccagctac acaatccgc gtgatctgc tgcgtcctc ttcatcttc atcaccgag atctgcctg gcgcactac atctaccga ctgatcaac tcggaagtc cgcagcgac tacgtctcc aaagaggac ttccgccag ttcttcctc aacgagctg cgcgccgac caggccaag gacgaaatc gtgcggatt ctcatcctg caggagcac cagaagggc accaggagg cgacgcgat gccgacgat taccaaatg ctcaactcg ccgagcgac gagccgcat gtggagttc ttcagtccg gagatgagg agcacgctg gagaagaag gacgagttg ataaggcag cagacgggg aacaagggg gcggctcct ggaggcgat tcctggatc tggctcacg agctacagc cggattccg gaaaaggca ttggagggc tactgcaag aaggtgcac cagttttgc cctccaggt cggcctggt cccacagga cctccggga agtcaagga cctaaaggc gaccgcgga gacaagggt tttcctggc gtgccgggc caaatgggt caccgaggt ttaccaggc cttccggga gaaaaagga tcgcaaggt cgtcctgga ttggatggt cgggatggt attccgggt gagccaggc ctggatgga atgcctgga cgcaatggt tacgacggt attcctggg gttaacggt agacctggg ttaccgggg agagacggg cgcaatgga accgatgga cttcccggc ttgccaggc cctcaaggc cctccaggc cccccaggc cctaaggga cttacgggc cctaaaggg aagcccggc cgacacggc cgtgatgga gatccggga aagccaggc gggacgttt tatcaaatc aacggaaaa aacgtctcc gagttgctg ataccgccc tcgattgca ggggctcaa gccactccg atgggaccc atcgtggta cacgaggga gaaaatgtt cgattgcac tgctcggcg atcggcact cccaacccc cacgtgacc tggatgaag ctggggaag cggcccgtc agcaggggg gcctggcat gatacggga gtttcaggc cacacgttg aacataacg agggttaac cgagtgcac atgggccag tacaagtgc attgctgat aacggcatt ccgcctcaa gcgagccaa acttttaat ttggaagtc tattttccg ccccttatc cggatatac aatcaggtg gttgaagtg ccgactgga tcttcggcc gttcttgaa tgcgaaacc gaggcgttc ccggaatct attagatac tgggagaga tccgatgga cgtctcttg gaaaacgga aataaatat caaattgac aactcgcta gacagggat ctctacaaa gcgagaatg cagctgaac ataaccaga gtacattca aacgacatg acttataaa tattactgc gtcagtaag aacgagctg aaaacaatc agaggagaa ctccagatt caagaacaa cagccaggc agaccgcgc cagcaactt aacgacaag aaaaccagc atttttggc aatttgccc cccattcaa gtctcggag gaagacctg tgtggcccc cctgtccag tgtccggag tgtgaagct tgcagtgcg agcggtgtc tcgctaatc gacctcata agtcgctgg aacatcagg aaattcggc aacataatg tacgacaag gactacgca aacagaacc acaaattgc gttttatac gcggtgggc aaacccgtg taccaccgc tattcggac cacaacaat gggtgttgg atgcgcgat tcgaaccca ttggttgaa aaagacgcc gaaaagttc tgggtgacc caggaggac gacccaagc cacctgttc cagttcgat aacaagacc atgtttcgg cgaaacgcc ccatccaga acctatgtg ctgagttat ccgtttcag gggaacgca cacgtcatt tataacgga tcgttctat tacaacgct aaagcgtcc aacaaaata atcaaattt aatctgcag acgagccac tcggataat ttgagcttg ccctttcct taccccctt aattcgtcg aatctctac acagcgcag tacaactac atggatttc agcgttgac gacaacgga ttgtgggtt atttttccc gttccggat tcgaacaac acggcggtt ttgaagatg gatgtaaac ttgatgaaa atcaactac atctggaac gtaagcata aaccacaat aaggtgggt gaaatgttc atcgtttgt ggagtgcta tacgcagtg gacagcgta acagacagg aacacgaaa attcgattc gccctcgat ctgtacaac gagaagctt ctcgatgtt aatcttgca ttttcgaac ccttttagg aaaacaaca atgatagga tacaaccac cggaataag gagctgtat acgtgggat aagggcaat caattgact tatcccgtg cggtatcac gagtcgggt tataacatg acggacagg gacgataaa attttcgca agcgaattt gcgcaaagg gatatgcct acagggtac aacgttttc cattgaggg acgacttct tacagtaga gtagatctc cgttttaga taatttgta ctaattgta atcaatggt gtttgtggc ccttgtaaa taaaaactg tttttttct
