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Gene information
General information
TC009365 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid46: 943265..944488
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009365
    iBeetle screen iB_01539
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellarium thinner - Number of animals showing the phenotype: 4
    vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber mostlynot present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    remarks - cuticles: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009365 RA
      MKVFIILAL AIYGTSASL SKDFVEEKW ESFKKTHEK 
      SYLNAKEEA FRKQIFQKK LERIEAHNE RFNKGLETY 
      TMGINMFTD MTPEEMRPY THGLIEPAV VPKPLVEIK 
      SRADLGLNH SVQYPASFD WRDKGMVTG VKNQGGCGS 
      CWAFSSTGA IESQVKIAK GANTDISVS EQQLVDCDT 
      AADGCGGGW MTDAFTYIA QTGGIDSES SYPYKGVDE 
      SCHFMSDKV AAKLKGYAY LTGPDENML ADMVSSKGP 
      VSVAFDAEG DFGSYSGGV YYNPNCATN KFTHAVLIV 
      GYGNENGQD YWLVKNSWG DGWGEHGYF KIARNKGNH 
      CGIASKASY PVLA
    CDS sequence
    • >TC009365 RA
      atgaaagtg ttcataatt ctcgccctt gccatttac 
      ggcacctca gcttcgctg tccaaagat ttcgtcgaa 
      gaaaaatgg gaaagtttc aaaaaaacg cacgaaaaa 
      tcctatctt aatgccaag gaagaggcc ttccgtaag 
      caaatcttc cagaagaag ctcgagcgc attgaggcg 
      cacaatgag cggttcaac aaaggcttg gagacctac 
      accatgggc atcaacatg ttcaccgac atgaccccc 
      gaagaaatg cgaccatac acccatggt ctgatcgag 
      ccagcggtc gtgcccaaa cccctcgtt gaaatcaag 
      tcccgagct gacctgggt ctgaaccac agtgtgcaa 
      tacccagct agtttcgac tggagggac aaaggaatg 
      gtcacagga gtcaaaaac cagggaggt tgtggctcc 
      tgctgggcc ttctcaagt actggcgcc attgaatcg 
      caagtcaag atcgccaaa ggcgccaac accgacatc 
      tccgtcagc gagcagcag ctggtggac tgcgacacc 
      gccgccgac ggctgcggg ggcggctgg atgacggac 
      gccttcacc tacatcgcc cagactggg ggaattgac 
      tccgaaagc tcgtatcct tataaggga gttgacgaa 
      agttgtcac ttcatgtcg gacaaagtc gcagctaag 
      ctcaaagga tatgcttat ctgaccggg cctgatgag 
      aatatgttg gccgatatg gtgtccagc aaaggccct 
      gttagtgta gctttcgac gctgaaggt gatttcggg 
      tcttacagc ggtggggtt tattataat cccaactgt 
      gctaccaat aaattcacc catgctgta ctcatcgtt 
      gggtatggg aatgagaat gggcaggat tactggttg 
      gtgaagaat tcatggggc gacggttgg ggcgaacat 
      gggtatttc aaaatcgcc aggaataag ggcaatcac 
      tgcgggatt gccagcaag gctagttat ccagtcttg 
      gcgtaa
    mRNA sequence
    • >TC009365 RA
      ctcaaaaaa cgagccaca atgaaagtg ttcataatt 
      ctcgccctt gccatttac ggcacctca gcttcgctg 
      tccaaagat ttcgtcgaa gaaaaatgg gaaagtttc 
      aaaaaaacg cacgaaaaa tcctatctt aatgccaag 
      gaagaggcc ttccgtaag caaatcttc cagaagaag 
      ctcgagcgc attgaggcg cacaatgag cggttcaac 
      aaaggcttg gagacctac accatgggc atcaacatg 
      ttcaccgac atgaccccc gaagaaatg cgaccatac 
      acccatggt ctgatcgag ccagcggtc gtgcccaaa 
      cccctcgtt gaaatcaag tcccgagct gacctgggt 
      ctgaaccac agtgtgcaa tacccagct agtttcgac 
      tggagggac aaaggaatg gtcacagga gtcaaaaac 
      cagggaggt tgtggctcc tgctgggcc ttctcaagt 
      actggcgcc attgaatcg caagtcaag atcgccaaa 
      ggcgccaac accgacatc tccgtcagc gagcagcag 
      ctggtggac tgcgacacc gccgccgac ggctgcggg 
      ggcggctgg atgacggac gccttcacc tacatcgcc 
      cagactggg ggaattgac tccgaaagc tcgtatcct 
      tataaggga gttgacgaa agttgtcac ttcatgtcg 
      gacaaagtc gcagctaag ctcaaagga tatgcttat 
      ctgaccggg cctgatgag aatatgttg gccgatatg 
      gtgtccagc aaaggccct gttagtgta gctttcgac 
      gctgaaggt gatttcggg tcttacagc ggtggggtt 
      tattataat cccaactgt gctaccaat aaattcacc 
      catgctgta ctcatcgtt gggtatggg aatgagaat 
      gggcaggat tactggttg gtgaagaat tcatggggc 
      gacggttgg ggcgaacat gggtatttc aaaatcgcc 
      aggaataag ggcaatcac tgcgggatt gccagcaag 
      gctagttat ccagtcttg gcgtaaatg atacatcga 
      tatattgtg ttaaacttt tattaaata aaataaaaa 
      ctaaaca