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Gene information
General information
TC009354 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 781156..790143
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009354
    iBeetle screen iB_01536

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009354 RA
      MFSTSTQCK NWMFNSEDE LNKLREAAN LKHIQAYGR 
      HINEAARFE FFLTAEEEK IMVKRYEIR LRDFCKRFQ 
      PPMTRCVIG TAFHYFKRF YIHNSVMNH HPKEIMVTC 
      IYLACKVEE FNVSIQQFV ANLKGDREK ATDIILNNE 
      LLLMEQLNF QLSIHNPFR PVEGLLIDI KTRCALNDP 
      ERLRPGTEH FLERAFLTD VVLLYSPSQ VALAAVLHA 
      ASKLQENLD SYVTDTLFG VEGSGKLAE LIEAVRAIR 
      SIVKMVDAT PDKNQQKLL DKKLEKCRN PENNPDSEI 
      YKKRMQALL DEDDDLYHR ISTRTDSLD NSALGMSVG 
      GSPEVA
    CDS sequence
    • >TC009354 RA
      atgttttcc acgagcacg cagtgtaaa aattggatg 
      tttaacagc gaagatgag ttaaataaa ttacgcgaa 
      gctgcgaat ttgaagcat atccaagcc tacggccgc 
      cacatcaat gaagccgct cgtttcgag ttttttctc 
      actgccgag gaggaaaag ataatggtg aaacgttac 
      gaaatccga ttacgcgat ttttgtaaa cggtttcaa 
      cctccgatg acgcggtgt gtgatcggg actgcgttc 
      cattatttc aagcgtttt tacattcac aattcggta 
      atgaaccac catcctaag gagataatg gtcacgtgt 
      atttattta gcgtgtaag gttgaagag ttcaatgtt 
      tccattcag caatttgtt gccaatctc aaaggcgac 
      agggagaag gccactgat attattttg aacaacgag 
      ttgttgctg atggagcag ctgaatttc cagttgtcg 
      attcacaat ccgttcagg ccagtggag ggactacta 
      attgatatt aaaactaga tgtgcatta aatgatcct 
      gaacgttta aggcctgga actgaacat tttctcgag 
      agagcattt ttaacagac gttgtgtta ttgtattct 
      ccgagtcaa gtggcacta gctgctgtt ttacacgca 
      gcctcaaaa ttgcaagaa aatttagat tcttacgtc 
      actgataca ctatttggg gttgaggga agtggcaaa 
      ttggctgaa ttaattgaa gccgtgcga gctatccga 
      tctattgtt aaaatggtc gacgctacg cctgataaa 
      aatcaacag aaactttta gacaagaaa ttggagaag 
      tgtaggaat cctgaaaat aatcccgat agtgaaata 
      tataagaaa cgaatgcag gctttgctg gacgaagat 
      gacgatttg tatcaccgt atttcgaca aggactgat 
      tctttggat aactccgcc ttaggcatg tcagtgggt 
      ggttcaccc gaagtagcg taa
    mRNA sequence
    • >TC009354 RA
      ttttggtta ttggttcaa tgataataa tgccattaa 
      tcaaggcat catcagatg cactttcct cgctctgta 
      attaaaaaa gtcatatat gtcaagtat ggaaccttg 
      ctggtcttt taattcgta tccaagtcc caagtaaaa 
      ggattattt atcgtacca acatcctgc aacggtcac 
      aacgctact aattagccg actacctgt atcaaaaga 
      aaaaatcgt aactgctca ttatcggag tcctcctca 
      gtggacttt tccgtgaaa ttttcgaca atatttcaa 
      atcggtgtt tgagaaaag agtgaaaac aataatttc 
      catctaccc ttcccgctt attattaat caaaagctt 
      agcaccccc gaattagag gctccgttt cccgaaaac 
      tatcaaaga gaatattaa ttaggccat ctcctgcca 
      ctccacaat taccacacg gttaaaaaa ggaaaagtg 
      gcaattaaa aattaccct aaaacatat tttagcaaa 
      gacggcagt agctcagct agccggtaa ttaaacact 
      gcgcttaac gtttaagtt aatcattag ttcactgtg 
      cacaccacc agagggccc ctcgattac agttttctt 
      taaataaag tgttacaaa tttgcgaga gaggtcggc 
      acgttgcgt gacagccgt catgtgtca aactagcac 
      gtaacctaa ctaaacaaa tttcaaaat cgctcaaaa 
      tatgttttc cacgagcac gcagtgtaa aaattggat 
      gtttaacag cgaagatga gttaaataa attacgcga 
      agctgcgaa tttgaagca tatccaagc ctacggccg 
      ccacatcaa tgaagccgc tcgtttcga gttttttct 
      cactgccga ggaggaaaa gataatggt gaaacgtta 
      cgaaatccg attacgcga tttttgtaa acggtttca 
      acctccgat gacgcggtg tgtgatcgg gactgcgtt 
      ccattattt caagcgttt ttacattca caattcggt 
      aatgaacca ccatcctaa ggagataat ggtcacgtg 
      tatttattt agcgtgtaa ggttgaaga gttcaatgt 
      ttccattca gcaatttgt tgccaatct caaaggcga 
      cagggagaa ggccactga tattatttt gaacaacga 
      gttgttgct gatggagca gctgaattt ccagttgtc 
      gattcacaa tccgttcag gccagtgga gggactact 
      aattgatat taaaactag atgtgcatt aaatgatcc 
      tgaacgttt aaggcctgg aactgaaca ttttctcga 
      gagagcatt tttaacaga cgttgtgtt attgtattc 
      tccgagtca agtggcact agctgctgt tttacacgc 
      agcctcaaa attgcaaga aaatttaga ttcttacgt 
      cactgatac actatttgg ggttgaggg aagtggcaa 
      attggctga attaattga agccgtgcg agctatccg 
      atctattgt taaaatggt cgacgctac gcctgataa 
      aaatcaaca gaaactttt agacaagaa attggagaa 
      gtgtaggaa tcctgaaaa taatcccga tagtgaaat 
      atataagaa acgaatgca ggctttgct ggacgaaga 
      tgacgattt gtatcaccg tatttcgac aaggactga 
      ttctttgga taactccgc cttaggcat gtcagtggg 
      tggttcacc cgaagtagc gtaatattg tgtactgta 
      tttcaattt tgtgaataa aaactataa cttaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY