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Gene information
General information
TC009349 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 653057..669184
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009349
    iBeetle screen iB_01534

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: bad quality of preparation,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009349 RA
      MHSASFDSE ISGKDGGEV LVAIAALVT EGRIPGKLS 
      SKTKGFYEG PHCPLNKTA REHRCDLSN LLCSTYENV 
      RLEVLDLWK KNIPVCLEV LLTCPCKNN VTVKSPETY 
      DDDFLLEKW YFSINHRRS AEPLFFNFV QLMNAIRSQ 
      LYFSQLTAW LTVQDENSP IKITDKNLR YRLTLPGET 
      FESSQFTQS AVKHEFPLT DIGNGFTAT VSFFSLPRM 
      SALPEFACL KKIHEGSSL VDDRMHTVC TLKGKHRCE 
      DFEEVENVK SQRAIKITR FKKPHPSTS RPKLTVDVH 
      KANTYQEKN LLNSHKNDL KVQDKGKLL LDAIERSAL 
      KSTNERRCD ENCVQTERN DCDTSERDI SSIKGQSKY 
      DIVVETCDS PKARHKTDV ATNIEHAIK GMRKKLTLD 
      DEVGKSEAE IPSAFEQAK FRKDLDNAA SMVFHSRTG 
      LPLTSSPAP VRRGKSCFD FDSSINSVS AIKSALFPT 
      SFSTDEDSE SDGSIVSPC SPETNTVPK RELSPVKYR 
      RKGHAANLL GSFEESVLN GRLEPVSTV HGFTAELGA 
      SGSFVPKHL VVPVTVFFY TLGDNDKVS SPYLGHINL 
      GKKGYNVPK TGTVQVTLF NPLGTVVKM FVIMYDLSD 
      MPPNSQTFI RQRTLYMPT NYKEANAEW GPKWLRYLV 
      HLRFISSKS GKIYLHSDI RMIIFRKSD LDTATAHGI 
      DMSYELRSF THMPVNPRF SPRK
    CDS sequence
    • >TC009349 RA
      atgcatagc gcgagcttt gatagtgag attagcggc 
      aaggatgga ggagaggtt ttggtggcg attgctgct 
      ttggtgacg gaaggtcga attccgggc aagctgagc 
      agcaaaacg aagggcttc tatgaaggg ccgcattgt 
      cccttgaat aagacagcg agagagcat aggtgcgac 
      ctatccaat ctattatgc tcaacctac gaaaatgtg 
      cgtttggag gttttggat ctatggaag aaaaacatt 
      cctgtctgt ttggaagtg cttctgact tgtccttgt 
      aaaaataac gtgactgta aaatcaccc gaaacatac 
      gacgacgat tttctcttg gaaaagtgg tatttcagc 
      atcaaccat cgaagaagt gccgaacca ctgtttttc 
      aattttgtc caactgatg aacgcaatc cggtcccaa 
      ttgtatttt tcccagctc acggcctgg ctaacagtc 
      caggacgag aacagtcca attaaaata accgacaag 
      aacctccgg taccgtctg acattgccg ggagagacg 
      ttcgaatcg tcgcagttc acacaatct gccgtcaaa 
      cacgaattc ccgctcaca gatattggg aacgggttc 
      acagccact gtttccttc ttcagccta ccacggatg 
      tcagctttg cccgaattc gcgtgcttg aagaaaatc 
      cacgaaggg agtagtcta gtggacgac cgcatgcac 
      accgtgtgc accctcaag ggcaaacac cgctgcgaa 
      gacttcgaa gaagttgag aatgtgaag agtcaaagg 
      gcgattaaa atcacgagg ttcaagaag ccgcacccg 
      tccacctcc aggccgaag ctgacggtg gacgtgcac 
      aaggcgaac acctaccag gagaagaac ctcctcaac 
      agccataaa aatgatctc aaggtgcag gacaagggc 
      aagctcctg ctggacgcg atcgagcgg agcgctttg 
      aaaagtaca aacgagcga agatgtgat gaaaactgt 
      gttcaaacc gaacggaat gattgtgat acgagcgaa 
      cgtgatatt tctagtatt aagggtcaa agcaaatat 
      gacatagtt gtagaaact tgtgatagt cccaaagcg 
      agacacaaa actgatgtt gccacaaac atcgaacat 
      gcaataaaa gggatgagg aaaaagctg acactggac 
      gatgaggtt ggaaagagc gaggctgaa ataccgtca 
      gcgttcgaa caggcaaag tttcgcaaa gatctggat 
      aatgcagcg tcgatggtt ttccattca aggactggc 
      ttgccgctg actagcagt ccagcgcct gtccggaga 
      ggaaagtcg tgctttgat ttcgactct agtataaat 
      tcggtttca gcgatcaaa agcgcttta tttccgacc 
      agtttttcg acggatgag gatagtgag agtgatggt 
      agtattgtg tcgccttgc agtcccgaa actaacacc 
      gttccgaaa cgggaattg tcacccgtt aagtaccga 
      aggaaaggg catgcagct aacttgtta ggaagtttc 
      gaagagtcg gttttaaac ggccgtctc gagccagtg 
      tcaaccgtc catggcttc accgctgaa ttaggggcc 
      agtggttct ttcgttcct aaacatcta gttgttcct 
      gttactgta tttttttac actttgggt gataacgac 
      aaagtttcg tctccatac ctgggccac attaattta 
      ggcaaaaag ggctacaac gtcccaaag acaggcacc 
      gttcaagtc acgctgttc aacccttta gggactgtt 
      gtaaaaatg tttgttatc atgtacgat ctcagcgac 
      atgccgccc aactctcag acgttcatc cggcaaagg 
      acgctctac atgcccacc aattacaag gaggcgaac 
      gcggaatgg gggcctaag tggctgcgc tatctcgtt 
      catttgagg tttatcagt tccaagtcc ggaaagatt 
      tatctgcac agcgacata cggatgata attttccgt 
      aaaagtgac ctggacacg gccacagcg cacggcatt 
      gatatgagc tacgagctg cgcagcttc actcacatg 
      ccagtcaat cctaggttt tcccccagg aagtaa
    mRNA sequence
    • >TC009349 RA
      tgactagtt tcaggcgaa cctgcgagc tgtcaacgt 
      accacaaca aagtaggct ttgcaagtg cattgtctg 
      agaaactta gcgaaaaaa cgcaaaatg tgtacaaaa 
      agactaaaa aagttttac tacagtgtg gttatttag 
      catacgtgt aatttgtga agagattgg agtgcaaca 
      caaagatgc atagcgcga gctttgata gtgagatta 
      gcggcaagg atggaggag aggttttgg tggcgattg 
      ctgctttgg tgacggaag gtcgaattc cgggcaagc 
      tgagcagca aaacgaagg gcttctatg aagggccgc 
      attgtccct tgaataaga cagcgagag agcataggt 
      gcgacctat ccaatctat tatgctcaa cctacgaaa 
      atgtgcgtt tggaggttt tggatctat ggaagaaaa 
      acattcctg tctgtttgg aagtgcttc tgacttgtc 
      cttgtaaaa ataacgtga ctgtaaaat cacccgaaa 
      catacgacg acgattttc tcttggaaa agtggtatt 
      tcagcatca accatcgaa gaagtgccg aaccactgt 
      ttttcaatt ttgtccaac tgatgaacg caatccggt 
      cccaattgt atttttccc agctcacgg cctggctaa 
      cagtccagg acgagaaca gtccaatta aaataaccg 
      acaagaacc tccggtacc gtctgacat tgccgggag 
      agacgttcg aatcgtcgc agttcacac aatctgccg 
      tcaaacacg aattcccgc tcacagata ttgggaacg 
      ggttcacag ccactgttt ccttcttca gcctaccac 
      ggatgtcag ctttgcccg aattcgcgt gcttgaaga 
      aaatccacg aagggagta gtctagtgg acgaccgca 
      tgcacaccg tgtgcaccc tcaagggca aacaccgct 
      gcgaagact tcgaagaag ttgagaatg tgaagagtc 
      aaagggcga ttaaaatca cgaggttca agaagccgc 
      acccgtcca cctccaggc cgaagctga cggtggacg 
      tgcacaagg cgaacacct accaggaga agaacctcc 
      tcaacagcc ataaaaatg atctcaagg tgcaggaca 
      agggcaagc tcctgctgg acgcgatcg agcggagcg 
      ctttgaaaa gtacaaacg agcgaagat gtgatgaaa 
      actgtgttc aaaccgaac ggaatgatt gtgatacga 
      gcgaacgtg atatttcta gtattaagg gtcaaagca 
      aatatgaca tagttgtag aaacttgtg atagtccca 
      aagcgagac acaaaactg atgttgcca caaacatcg 
      aacatgcaa taaaaggga tgaggaaaa agctgacac 
      tggacgatg aggttggaa agagcgagg ctgaaatac 
      cgtcagcgt tcgaacagg caaagtttc gcaaagatc 
      tggataatg cagcgtcga tggttttcc attcaagga 
      ctggcttgc cgctgacta gcagtccag cgcctgtcc 
      ggagaggaa agtcgtgct ttgatttcg actctagta 
      taaattcgg tttcagcga tcaaaagcg ctttatttc 
      cgaccagtt tttcgacgg atgaggata gtgagagtg 
      atggtagta ttgtgtcgc cttgcagtc ccgaaacta 
      acaccgttc cgaaacggg aattgtcac ccgttaagt 
      accgaagga aagggcatg cagctaact tgttaggaa 
      gtttcgaag agtcggttt taaacggcc gtctcgagc 
      cagtgtcaa ccgtccatg gcttcaccg ctgaattag 
      gggccagtg gttctttcg ttcctaaac atctagttg 
      ttcctgtta ctgtatttt tttacactt tgggtgata 
      acgacaaag tttcgtctc catacctgg gccacatta 
      atttaggca aaaagggct acaacgtcc caaagacag 
      gcaccgttc aagtcacgc tgttcaacc ctttaggga 
      ctgttgtaa aaatgtttg ttatcatgt acgatctca 
      gcgacatgc cgcccaact ctcagacgt tcatccggc 
      aaaggacgc tctacatgc ccaccaatt acaaggagg 
      cgaacgcgg aatgggggc ctaagtggc tgcgctatc 
      tcgttcatt tgaggttta tcagttcca agtccggaa 
      agatttatc tgcacagcg acatacgga tgataattt 
      tccgtaaaa gtgacctgg acacggcca cagcgcacg 
      gcattgata tgagctacg agctgcgca gcttcactc 
      acatgccag tcaatccta ggttttccc ccaggaagt 
      aactagaag tcaaattag aagaagaat caccacgaa 
      ttcccagaa gcgtattca atctacatt atcatattt 
      gctgttcgt tatttagtt gtttcgtgc agaagtgat 
      ggatttaga ggtttcttt gcgaatatt cgttaggaa 
      aatatagct cacaactgc cattgttgt aaatatatg 
      taaatattg attctgttt ggattttta aggacaata 
      ctggtaaac atcggttca tttgacgaa gtcttagaa 
      ttaggcgtt gattatttt ttaactacg tggaattgg 
      caacttcaa atttttaga gtagcacca gtatctaca 
      tcatattga caacatata aagttataa ttattatga 
      gtatcagag atatttatt ataatagaa ttatactaa 
      ttttgtatt agtaatgaa aataaatgt gcaaaaata 
      tc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY