Status
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Gene information
General information
TC009347 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 649805..652832
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009347
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01533

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009347 RA
      MSQEINGAK EEKAYFLSV LSTFESYRE QSLLRIDHK 
      QKCLCTLPP YHKKWLTKY KEDLEAFKE CIEKNSKFI 
      PIVLQHAHT IFDNVYSND GSSHSEIKV GTLAEGLDK 
      VQSVFKQLM RDWSALGAA ERAQCYQPI IDEIILQFP 
      EDKFVRSNI NVLVPGAGL GRLAFEIAT RGFACQGNE 
      FNLFMLIVS YYVLNHCKE VDEYTIYPW IHQYCNNLN 
      AKHQMTSVR FPDVKPMPT PESKFSMTA GDFLEVYTD 
      PDIWHCVAT CFFIDCAPN VVQFIETIY HILKPGGVW 
      INLGPLLYH YSDVKNEKS VEPSFEVVC EVIKNIGFE 
      MEKCKTGVK TKYCQNPRS MLQYEYDSV YFVCRKPIK 
      ITNGNNGTV DE
    CDS sequence
    • >TC009347 RA
      atgagtcaa gaaatcaac ggtgcaaaa gaagagaaa 
      gcctatttc ttgtcggtg ctttccacc tttgagagt 
      tacagagag cagtctttg ctgcgaatc gaccacaag 
      cagaaatgc ctctgcacg cttcctcca tatcacaaa 
      aaatggctc acgaagtac aaggaagac ttggaggcg 
      ttcaaggaa tgcattgaa aaaaattct aaatttatc 
      cctatcgtg ctccagcat gcccacacg atttttgat 
      aatgtctac agtaacgac ggctcctcg cattctgaa 
      attaaagtc gggactttg gccgaaggg ctggacaag 
      gtccagtcg gttttcaag cagttgatg cgggattgg 
      agcgctttg ggggctgcc gaaagggcc cagtgctac 
      caaccgatc attgacgaa attattctg cagtttcct 
      gaagacaaa tttgtcagg tcaaatatc aatgttttg 
      gtgcccggg gccgggtta ggtcgactg gcttttgaa 
      attgcaaca agggggttt gcctgccaa ggcaatgaa 
      tttaatttg tttatgcta attgtctcg tactacgtc 
      ctaaaccat tgcaaagag gttgatgaa tacactatt 
      tacccatgg atacatcag tattgtaat aacttgaat 
      gccaagcat caaatgaca agcgttagg tttcccgat 
      gttaagcca atgccaact cctgagagt aaattttca 
      atgactgct ggcgacttt ttggaggtt tacacagat 
      cccgacatc tggcattgc gtcgcaact tgttttttt 
      attgattgt gctccaaat gttgtccaa ttcattgag 
      accatctat catattttg aagccaggt ggcgtttgg 
      atcaatttg gggcctttg ttgtatcac tattccgat 
      gtaaaaaat gagaaaagt gttgaaccg agctttgaa 
      gttgtgtgt gaagtcatc aaaaatata ggtttcgaa 
      atggaaaag tgcaaaact ggcgttaaa acaaagtac 
      tgccagaac cccagatcc atgctccag tatgagtac 
      gatagcgtt tactttgtt tgtcggaaa cctatcaaa 
      attaccaac ggtaacaac ggaactgtg gatgaataa
    mRNA sequence
    • >TC009347 RA
      ctaaatcaa attaaaacg ggctttgtg aatttacat 
      atgattttg agcgttttt attttggat tttttgtgt 
      cggtggggt aggaatctg caatgcggc tcgatttgc 
      gaacatggc cgcttgggg atcgctgag ttatttagg 
      ttagaatcg gcaccgcaa gtagaaata agtactttt 
      aagcccgtg taatgtgat attagtaaa tagtgatta 
      tcaaagccg aatcagtta acagtttcc gtcaggatg 
      agtcaagaa atcaacggt gcaaaagaa gagaaagcc 
      tatttcttg tcggtgctt tccaccttt gagagttac 
      agagagcag tctttgctg cgaatcgac cacaagcag 
      aaatgcctc tgcacgctt cctccatat cacaaaaaa 
      tggctcacg aagtacaag gaagacttg gaggcgttc 
      aaggaatgc attgaaaaa aattctaaa tttatccct 
      atcgtgctc cagcatgcc cacacgatt tttgataat 
      gtctacagt aacgacggc tcctcgcat tctgaaatt 
      aaagtcggg actttggcc gaagggctg gacaaggtc 
      cagtcggtt ttcaagcag ttgatgcgg gattggagc 
      gctttgggg gctgccgaa agggcccag tgctaccaa 
      ccgatcatt gacgaaatt attctgcag tttcctgaa 
      gacaaattt gtcaggtca aatatcaat gttttggtg 
      cccggggcc gggttaggt cgactggct tttgaaatt 
      gcaacaagg gggtttgcc tgccaaggc aatgaattt 
      aatttgttt atgctaatt gtctcgtac tacgtccta 
      aaccattgc aaagaggtt gatgaatac actatttac 
      ccatggata catcagtat tgtaataac ttgaatgcc 
      aagcatcaa atgacaagc gttaggttt cccgatgtt 
      aagccaatg ccaactcct gagagtaaa ttttcaatg 
      actgctggc gactttttg gaggtttac acagatccc 
      gacatctgg cattgcgtc gcaacttgt ttttttatt 
      gattgtgct ccaaatgtt gtccaattc attgagacc 
      atctatcat attttgaag ccaggtggc gtttggatc 
      aatttgggg cctttgttg tatcactat tccgatgta 
      aaaaatgag aaaagtgtt gaaccgagc tttgaagtt 
      gtgtgtgaa gtcatcaaa aatataggt ttcgaaatg 
      gaaaagtgc aaaactggc gttaaaaca aagtactgc 
      cagaacccc agatccatg ctccagtat gagtacgat 
      agcgtttac tttgtttgt cggaaacct atcaaaatt 
      accaacggt aacaacgga actgtggat gaataaatt 
      gttgtagtt aaatgactg aattcaagt acaatattg 
      attaactgg aaattatat gtaaatcta cgatgttat 
      ttttgaaat gtttctaga tcggcgttt agttgcagt 
      gttgtaaac atcccagat ctgatataa ttttttcgt 
      aggaacatc cagtacaaa tgttcaact ttcaattgt 
      attaattca tttctcgtt tagtttatt ttttctatt 
      gaatcgaat ttaagtttc actatgtgt tgtttagca 
      tttaataaa taattgacc ccactttcc ctgaataaa 
      agacatttg gcgggaacg gccattttt atttcaaaa 
      cctagggag ttttcgctt ggttcgttt tagacatac 
      gaaggaatc tgtcactgt cagttgtta ttcaggttt 
      ttgtacaaa attatagaa aataaacat tttttagtg 
      gattaacta aatttacag gtaaaaaga ctactctca 
      tttaatgag atattaccg atttttcaa aaaaacatg 
      ttctgaaat ttgaggtta gtatcattt tttaaagca 
      tgtcgagtc gtgaaggag gtaagaaaa aacccctca 
      aacaaccca aaaaagaac agaaggaga tggatgacg 
      acgacctgg cccacaaac aaaaactga aggagcagc 
      aaaaagcgc tacaagagg ccaaagcac gagcgtctc 
      aaaaggggc ccatggtcg gtggtggca tcaaaaagt 
      ccggcaaaa agtgacaca ttctgaatc aacaatcat 
      taatgttaa ttttgtaaa ataaacagt ttcattgcc 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY