Status
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Gene information
General information
TC009343 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 593045..597760
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009343
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01532

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009343 RA
      MLILSILLT LLALVHQSA SINLTYIVK KEGDTLQPN 
CIAEENERY AAIGWIYPL SRTNQEPRI TNKYVPTIQ 
NNFNCNFAK NSKCNNIWA LKNWEIDTN STKTLDLLF 
DARWDNFPT ILTGANSKE FEDKIEIPT IFKAGFSLR 
ASASVELLI CDGWNPYNH PCYHLNMSQ NAIFLRKYM 
TLPKNISVS DKLLDSYQA FAKILSGDE WRNFVISLD 
DKGAFSLTD VSVNRTVIQ YEDKEALKP LYLLVRSAQ 
VALWKIHQN QFFYTKTVQ TSRFGPLLH LPSKFLCVS 
LYVSTCPTC SMTFFIIKD GQKKILKDV QPTQDFQWR 
LVKIKDDNV ESDKLNIFV ETKSANNDG FWAVDDVRV 
CHENEVKVT FLKLENGLK EDETDEVSC QVVEHPKWR 
PKKLVYSEI KDFPTVDNS SNSSSIYLN WTQEDPNNQ 
INYFITYEA NDLCPPENP NLERVKSNG FLMTKHNEI 
TINDLIPYT RYNITFSSM LHEKEKRIF VTTLESAQL 
SLEEIPSKL QIEVHDTQA YASWDKIAC KSIYGPVLY 
ALNLTNVKT NITKELGVQ TEDTVEIND LQPFTTYML 
EITTARNFE NIQNNKNTL KIENKFTTQ PGKAPPVEN 
LEIYSIGKN TVSLRYDLP RNSRGVPVY GQVTRCNAL 
TFKKCKTSN FPITPCKLW PRKYCIEMN NLIPYQNYT 
FKVSIRNER TQFYGDDVL VEAFLVEQV PGSPSNITY 
KIVDCKESL DYCHLNVSW MHPYNQNGS IESFHIVLN 
GTDNDTANS IQEVLKIFN SSYYVPRYE YQIKYIPYS 
THYELFVQS ANSKYKSPF AHVHVKTDD LGEHIDQNP 
ILLKSEPTS VTFTLPTLD HRLKSYSLV VIVQDFNQN 
ITIPTKITK NSKISDHLC HDFGDTWIL QKLEVATHN 
QAKNLEIGT DKPLKPNTQ YCFTFIVTN KYRNSEHDV 
VYYKKLTTP QSEISESGS NGLYALFLL LLLIPIGFF 
AYRYFKKRT PRRKRQQEN NKENVYESL PFEETEENC 
VSNDTYDHL LHK
    CDS sequence
    • >TC009343 RA
      atgctcatc ttaagcatc ctcctcacc ctattggca 
ctggtgcac caatcagcc agcatcaat ttgacttac 
attgtaaaa aaagaaggt gacacactt cagcccaat 
tgcatagct gaagagaac gaacgatat gctgcaata 
ggctggatc taccccctt tcaagaacc aatcaagaa 
ccgcgcata acgaataaa tacgtccct acgattcaa 
aacaacttc aattgcaac tttgccaaa aactccaag 
tgtaacaac atctgggcc ttgaaaaac tgggaaatt 
gacacgaac tcgaccaaa acgctcgat ttgctgttc 
gatgctcga tgggacaac ttccccacg attttaacc 
ggagctaac agcaaagaa ttcgaggat aaaatcgaa 
attcccaca attttcaaa gctgggttt tcgctacga 
gcgtccgcg agtgtcgag ttattaatt tgcgatggt 
tggaaccct tacaaccac ccttgctac cacttaaac 
atgagtcaa aatgcgatt tttctacgg aaatatatg 
actttgccg aagaatatt tcagtgagc gataaactg 
ttagattcg tatcaggcg ttcgctaag attttatcc 
ggtgatgag tggcgtaat ttcgtgata agtctggac 
gataaaggt gctttcagc ttaactgat gtcagtgtt 
aacagaact gttatccaa tacgaagat aaggaagcg 
ctaaaaccg ctctatttg ctcgtgcga agtgctcaa 
gttgcactg tggaaaata caccaaaac cagtttttt 
tacaccaaa acagtacaa acgtcacgt tttgggcct 
ttgttgcac ctcccttcg aaatttctc tgtgtgagt 
ttgtacgtc tcgacttgt cccacctgt tctatgact 
ttttttatc ataaaagac gggcaaaag aaaatttta 
aaggacgtg cagcccact caagatttt cagtggcgg 
ctcgtgaaa atcaaagac gataatgtt gaaagcgac 
aagcttaat atttttgtg gagacaaaa tcggcaaac 
aatgatggc ttttgggcg gtcgatgat gtgagagtg 
tgtcacgag aacgaggtg aaagtcacg tttttgaaa 
ctggaaaat gggctgaaa gaggacgaa actgatgaa 
gtttcgtgt caagtggtt gagcacccg aaatggagg 
cccaagaag ttggtttat tccgaaatc aaagatttt 
ccaacagta gataattcg tcaaattca tcatcgatt 
tacttaaac tggactcag gaagacccc aacaaccaa 
ataaactat ttcatcaca tacgaggcc aacgatttg 
tgccctcct gagaatcct aacttagaa agagtaaaa 
tcgaacggt tttctaatg acaaaacat aatgaaata 
acaataaac gacctcatt ccctacacc agatacaac 
atcacgttc tcgagcatg ctccacgag aaggagaaa 
cgaattttt gtgacaact ctagaatca gcccaatta 
tcactcgaa gaaattcct tcaaaacta caaatcgaa 
gtgcacgac acccaagcg tacgcatct tgggacaaa 
atcgcctgt aaatcaatt tacggcccg gttttatat 
gcacttaat ttaacgaat gttaagaca aacattacc 
aaagaattg ggagtgcaa actgaagat acggtcgag 
atcaacgat ttgcaacct tttaccacg tacatgctt 
gaaattaca acagcacgt aactttgaa aacatacaa 
aataacaaa aacacgctc aaaatcgag aacaagttc 
acgacacag ccagggaag gcaccacct gttgaaaat 
ttggaaatt tactcgatt ggtaaaaat acagtctcg 
cttcgttac gacttgccc aggaattcg cgaggggtt 
ccagtgtac gggcaagtg actcgttgc aatgctctt 
acgttcaaa aaatgcaaa acttcgaat tttcccata 
acgccgtgt aaattatgg ccgaggaaa tactgtatc 
gagatgaac aatttgatt ccgtatcaa aactacacg 
tttaaagtt agtatcagg aacgaaagg acgcagttt 
tatggtgat gatgtgttg gttgaggcg tttttggtg 
gaacaagtt ccggggtcg ccgtccaat ataacttac 
aaaatcgtt gactgtaag gaaagtttg gactattgt 
catttgaac gtgagctgg atgcacccc tacaaccaa 
aatggttca atcgaatcg tttcatatt gtactgaat 
ggtacagat aatgataca gcaaacagt attcaagaa 
gttttgaaa atattcaac tcgtcctac tacgtgcca 
cgatatgaa taccagatc aagtacata ccctatagc 
actcactac gaactattc gtccaatca gcaaactca 
aaatacaag agccctttt gcccacgtc cacgtcaaa 
acggacgat ttgggcgaa cacattgac caaaaccct 
atcttactc aaatccgag cccacttca gtaacgttc 
actttaccc acccttgac caccgtttg aaatcctac 
agtcttgtc gtaattgtc caagacttc aaccaaaac 
atcacaatt ccgacaaaa ataacgaaa aatagcaaa 
atcagcgac cacttgtgc cacgacttt ggcgacact 
tggattcta caaaaactg gaagtagcc acacataat 
caggccaaa aatttggaa attggaact gataagcct 
ttaaaaccc aacactcaa tattgcttc actttcatc 
gtcaccaac aaatacaga aatagcgaa cacgatgtg 
gtttattac aagaagttg acgacgcca caaagcgaa 
atttcggaa agtggttcg aacggcctc tacgctcta 
tttttgcta cttttgcta ataccgatc gggtttttc 
gcctatagg tatttcaaa aagagaaca cctagaagg 
aagcgtcaa caggagaac aataaggaa aacgtttac 
gaatccttg ccgtttgaa gaaaccgaa gaaaactgc 
gtcagtaac gacacttac gaccacttg ttacataaa 
tag
    mRNA sequence
    • >TC009343 RA
      gcgtgcatg aatttgaat gaaatcgtt tagcaaaat 
ggtccaaaa aaaacgggg taaaatgct catcttaag 
catcctcct caccctatt ggcactggt gcaccaatc 
agccagcat caatttgac ttacattgt aaaaaaaga 
aggtgacac acttcagcc caattgcat agctgaaga 
gaacgaacg atatgctgc aataggctg gatctaccc 
cctttcaag aaccaatca agaaccgcg cataacgaa 
taaatacgt ccctacgat tcaaaacaa cttcaattg 
caactttgc caaaaactc caagtgtaa caacatctg 
ggccttgaa aaactggga aattgacac gaactcgac 
caaaacgct cgatttgct gttcgatgc tcgatggga 
caacttccc cacgatttt aaccggagc taacagcaa 
agaattcga ggataaaat cgaaattcc cacaatttt 
caaagctgg gttttcgct acgagcgtc cgcgagtgt 
cgagttatt aatttgcga tggttggaa cccttacaa 
ccacccttg ctaccactt aaacatgag tcaaaatgc 
gatttttct acggaaata tatgacttt gccgaagaa 
tatttcagt gagcgataa actgttaga ttcgtatca 
ggcgttcgc taagatttt atccggtga tgagtggcg 
taatttcgt gataagtct ggacgataa aggtgcttt 
cagcttaac tgatgtcag tgttaacag aactgttat 
ccaatacga agataagga agcgctaaa accgctcta 
tttgctcgt gcgaagtgc tcaagttgc actgtggaa 
aatacacca aaaccagtt tttttacac caaaacagt 
acaaacgtc acgttttgg gcctttgtt gcacctccc 
ttcgaaatt tctctgtgt gagtttgta cgtctcgac 
ttgtcccac ctgttctat gactttttt tatcataaa 
agacgggca aaagaaaat tttaaagga cgtgcagcc 
cactcaaga ttttcagtg gcggctcgt gaaaatcaa 
agacgataa tgttgaaag cgacaagct taatatttt 
tgtggagac aaaatcggc aaacaatga tggcttttg 
ggcggtcga tgatgtgag agtgtgtca cgagaacga 
ggtgaaagt cacgttttt gaaactgga aaatgggct 
gaaagagga cgaaactga tgaagtttc gtgtcaagt 
ggttgagca cccgaaatg gaggcccaa gaagttggt 
ttattccga aatcaaaga ttttccaac agtagataa 
ttcgtcaaa ttcatcatc gatttactt aaactggac 
tcaggaaga ccccaacaa ccaaataaa ctatttcat 
cacatacga ggccaacga tttgtgccc tcctgagaa 
tcctaactt agaaagagt aaaatcgaa cggttttct 
aatgacaaa acataatga aataacaat aaacgacct 
cattcccta caccagata caacatcac gttctcgag 
catgctcca cgagaagga gaaacgaat ttttgtgac 
aactctaga atcagccca attatcact cgaagaaat 
tccttcaaa actacaaat cgaagtgca cgacaccca 
agcgtacgc atcttggga caaaatcgc ctgtaaatc 
aatttacgg cccggtttt atatgcact taatttaac 
gaatgttaa gacaaacat taccaaaga attgggagt 
gcaaactga agatacggt cgagatcaa cgatttgca 
accttttac cacgtacat gcttgaaat tacaacagc 
acgtaactt tgaaaacat acaaaataa caaaaacac 
gctcaaaat cgagaacaa gttcacgac acagccagg 
gaaggcacc acctgttga aaatttgga aatttactc 
gattggtaa aaatacagt ctcgcttcg ttacgactt 
gcccaggaa ttcgcgagg ggttccagt gtacgggca 
agtgactcg ttgcaatgc tcttacgtt caaaaaatg 
caaaacttc gaattttcc cataacgcc gtgtaaatt 
atggccgag gaaatactg tatcgagat gaacaattt 
gattccgta tcaaaacta cacgtttaa agttagtat 
caggaacga aaggacgca gttttatgg tgatgatgt 
gttggttga ggcgttttt ggtggaaca agttccggg 
gtcgccgtc caatataac ttacaaaat cgttgactg 
taaggaaag tttggacta ttgtcattt gaacgtgag 
ctggatgca cccctacaa ccaaaatgg ttcaatcga 
atcgtttca tattgtact gaatggtac agataatga 
tacagcaaa cagtattca agaagtttt gaaaatatt 
caactcgtc ctactacgt gccacgata tgaatacca 
gatcaagta catacccta tagcactca ctacgaact 
attcgtcca atcagcaaa ctcaaaata caagagccc 
ttttgccca cgtccacgt caaaacgga cgatttggg 
cgaacacat tgaccaaaa ccctatctt actcaaatc 
cgagcccac ttcagtaac gttcacttt acccaccct 
tgaccaccg tttgaaatc ctacagtct tgtcgtaat 
tgtccaaga cttcaacca aaacatcac aattccgac 
aaaaataac gaaaaatag caaaatcag cgaccactt 
gtgccacga ctttggcga cacttggat tctacaaaa 
actggaagt agccacaca taatcaggc caaaaattt 
ggaaattgg aactgataa gcctttaaa acccaacac 
tcaatattg cttcacttt catcgtcac caacaaata 
cagaaatag cgaacacga tgtggttta ttacaagaa 
gttgacgac gccacaaag cgaaatttc ggaaagtgg 
ttcgaacgg cctctacgc tctattttt gctactttt 
gctaatacc gatcgggtt tttcgccta taggtattt 
caaaaagag aacacctag aaggaagcg tcaacagga 
gaacaataa ggaaaacgt ttacgaatc cttgccgtt 
tgaagaaac cgaagaaaa ctgcgtcag taacgacac 
ttacgacca cttgttaca taaatagtt tttaacgta 
gttgtactt aaatgtgtt ctgtgataa caaacgtct 
cgtgtatat ttttactaa caaaactgt aattttaat 
gtgccatac taagtgttc aagtgccca caaaccaat 
tttgtaata cttaataaa ccataatat ccgag

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY