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Gene information
General information
TC009343 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 593045..597760
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009343
    iBeetle screen iB_01532

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009343 RA
      MLILSILLT LLALVHQSA SINLTYIVK KEGDTLQPN 
      CIAEENERY AAIGWIYPL SRTNQEPRI TNKYVPTIQ 
      NNFNCNFAK NSKCNNIWA LKNWEIDTN STKTLDLLF 
      DARWDNFPT ILTGANSKE FEDKIEIPT IFKAGFSLR 
      ASASVELLI CDGWNPYNH PCYHLNMSQ NAIFLRKYM 
      TLPKNISVS DKLLDSYQA FAKILSGDE WRNFVISLD 
      DKGAFSLTD VSVNRTVIQ YEDKEALKP LYLLVRSAQ 
      VALWKIHQN QFFYTKTVQ TSRFGPLLH LPSKFLCVS 
      LYVSTCPTC SMTFFIIKD GQKKILKDV QPTQDFQWR 
      LVKIKDDNV ESDKLNIFV ETKSANNDG FWAVDDVRV 
      CHENEVKVT FLKLENGLK EDETDEVSC QVVEHPKWR 
      PKKLVYSEI KDFPTVDNS SNSSSIYLN WTQEDPNNQ 
      INYFITYEA NDLCPPENP NLERVKSNG FLMTKHNEI 
      TINDLIPYT RYNITFSSM LHEKEKRIF VTTLESAQL 
      SLEEIPSKL QIEVHDTQA YASWDKIAC KSIYGPVLY 
      ALNLTNVKT NITKELGVQ TEDTVEIND LQPFTTYML 
      EITTARNFE NIQNNKNTL KIENKFTTQ PGKAPPVEN 
      LEIYSIGKN TVSLRYDLP RNSRGVPVY GQVTRCNAL 
      TFKKCKTSN FPITPCKLW PRKYCIEMN NLIPYQNYT 
      FKVSIRNER TQFYGDDVL VEAFLVEQV PGSPSNITY 
      KIVDCKESL DYCHLNVSW MHPYNQNGS IESFHIVLN 
      GTDNDTANS IQEVLKIFN SSYYVPRYE YQIKYIPYS 
      THYELFVQS ANSKYKSPF AHVHVKTDD LGEHIDQNP 
      ILLKSEPTS VTFTLPTLD HRLKSYSLV VIVQDFNQN 
      ITIPTKITK NSKISDHLC HDFGDTWIL QKLEVATHN 
      QAKNLEIGT DKPLKPNTQ YCFTFIVTN KYRNSEHDV 
      VYYKKLTTP QSEISESGS NGLYALFLL LLLIPIGFF 
      AYRYFKKRT PRRKRQQEN NKENVYESL PFEETEENC 
      VSNDTYDHL LHK
    CDS sequence
    • >TC009343 RA
      atgctcatc ttaagcatc ctcctcacc ctattggca 
      ctggtgcac caatcagcc agcatcaat ttgacttac 
      attgtaaaa aaagaaggt gacacactt cagcccaat 
      tgcatagct gaagagaac gaacgatat gctgcaata 
      ggctggatc taccccctt tcaagaacc aatcaagaa 
      ccgcgcata acgaataaa tacgtccct acgattcaa 
      aacaacttc aattgcaac tttgccaaa aactccaag 
      tgtaacaac atctgggcc ttgaaaaac tgggaaatt 
      gacacgaac tcgaccaaa acgctcgat ttgctgttc 
      gatgctcga tgggacaac ttccccacg attttaacc 
      ggagctaac agcaaagaa ttcgaggat aaaatcgaa 
      attcccaca attttcaaa gctgggttt tcgctacga 
      gcgtccgcg agtgtcgag ttattaatt tgcgatggt 
      tggaaccct tacaaccac ccttgctac cacttaaac 
      atgagtcaa aatgcgatt tttctacgg aaatatatg 
      actttgccg aagaatatt tcagtgagc gataaactg 
      ttagattcg tatcaggcg ttcgctaag attttatcc 
      ggtgatgag tggcgtaat ttcgtgata agtctggac 
      gataaaggt gctttcagc ttaactgat gtcagtgtt 
      aacagaact gttatccaa tacgaagat aaggaagcg 
      ctaaaaccg ctctatttg ctcgtgcga agtgctcaa 
      gttgcactg tggaaaata caccaaaac cagtttttt 
      tacaccaaa acagtacaa acgtcacgt tttgggcct 
      ttgttgcac ctcccttcg aaatttctc tgtgtgagt 
      ttgtacgtc tcgacttgt cccacctgt tctatgact 
      ttttttatc ataaaagac gggcaaaag aaaatttta 
      aaggacgtg cagcccact caagatttt cagtggcgg 
      ctcgtgaaa atcaaagac gataatgtt gaaagcgac 
      aagcttaat atttttgtg gagacaaaa tcggcaaac 
      aatgatggc ttttgggcg gtcgatgat gtgagagtg 
      tgtcacgag aacgaggtg aaagtcacg tttttgaaa 
      ctggaaaat gggctgaaa gaggacgaa actgatgaa 
      gtttcgtgt caagtggtt gagcacccg aaatggagg 
      cccaagaag ttggtttat tccgaaatc aaagatttt 
      ccaacagta gataattcg tcaaattca tcatcgatt 
      tacttaaac tggactcag gaagacccc aacaaccaa 
      ataaactat ttcatcaca tacgaggcc aacgatttg 
      tgccctcct gagaatcct aacttagaa agagtaaaa 
      tcgaacggt tttctaatg acaaaacat aatgaaata 
      acaataaac gacctcatt ccctacacc agatacaac 
      atcacgttc tcgagcatg ctccacgag aaggagaaa 
      cgaattttt gtgacaact ctagaatca gcccaatta 
      tcactcgaa gaaattcct tcaaaacta caaatcgaa 
      gtgcacgac acccaagcg tacgcatct tgggacaaa 
      atcgcctgt aaatcaatt tacggcccg gttttatat 
      gcacttaat ttaacgaat gttaagaca aacattacc 
      aaagaattg ggagtgcaa actgaagat acggtcgag 
      atcaacgat ttgcaacct tttaccacg tacatgctt 
      gaaattaca acagcacgt aactttgaa aacatacaa 
      aataacaaa aacacgctc aaaatcgag aacaagttc 
      acgacacag ccagggaag gcaccacct gttgaaaat 
      ttggaaatt tactcgatt ggtaaaaat acagtctcg 
      cttcgttac gacttgccc aggaattcg cgaggggtt 
      ccagtgtac gggcaagtg actcgttgc aatgctctt 
      acgttcaaa aaatgcaaa acttcgaat tttcccata 
      acgccgtgt aaattatgg ccgaggaaa tactgtatc 
      gagatgaac aatttgatt ccgtatcaa aactacacg 
      tttaaagtt agtatcagg aacgaaagg acgcagttt 
      tatggtgat gatgtgttg gttgaggcg tttttggtg 
      gaacaagtt ccggggtcg ccgtccaat ataacttac 
      aaaatcgtt gactgtaag gaaagtttg gactattgt 
      catttgaac gtgagctgg atgcacccc tacaaccaa 
      aatggttca atcgaatcg tttcatatt gtactgaat 
      ggtacagat aatgataca gcaaacagt attcaagaa 
      gttttgaaa atattcaac tcgtcctac tacgtgcca 
      cgatatgaa taccagatc aagtacata ccctatagc 
      actcactac gaactattc gtccaatca gcaaactca 
      aaatacaag agccctttt gcccacgtc cacgtcaaa 
      acggacgat ttgggcgaa cacattgac caaaaccct 
      atcttactc aaatccgag cccacttca gtaacgttc 
      actttaccc acccttgac caccgtttg aaatcctac 
      agtcttgtc gtaattgtc caagacttc aaccaaaac 
      atcacaatt ccgacaaaa ataacgaaa aatagcaaa 
      atcagcgac cacttgtgc cacgacttt ggcgacact 
      tggattcta caaaaactg gaagtagcc acacataat 
      caggccaaa aatttggaa attggaact gataagcct 
      ttaaaaccc aacactcaa tattgcttc actttcatc 
      gtcaccaac aaatacaga aatagcgaa cacgatgtg 
      gtttattac aagaagttg acgacgcca caaagcgaa 
      atttcggaa agtggttcg aacggcctc tacgctcta 
      tttttgcta cttttgcta ataccgatc gggtttttc 
      gcctatagg tatttcaaa aagagaaca cctagaagg 
      aagcgtcaa caggagaac aataaggaa aacgtttac 
      gaatccttg ccgtttgaa gaaaccgaa gaaaactgc 
      gtcagtaac gacacttac gaccacttg ttacataaa 
      tag
    mRNA sequence
    • >TC009343 RA
      gcgtgcatg aatttgaat gaaatcgtt tagcaaaat 
      ggtccaaaa aaaacgggg taaaatgct catcttaag 
      catcctcct caccctatt ggcactggt gcaccaatc 
      agccagcat caatttgac ttacattgt aaaaaaaga 
      aggtgacac acttcagcc caattgcat agctgaaga 
      gaacgaacg atatgctgc aataggctg gatctaccc 
      cctttcaag aaccaatca agaaccgcg cataacgaa 
      taaatacgt ccctacgat tcaaaacaa cttcaattg 
      caactttgc caaaaactc caagtgtaa caacatctg 
      ggccttgaa aaactggga aattgacac gaactcgac 
      caaaacgct cgatttgct gttcgatgc tcgatggga 
      caacttccc cacgatttt aaccggagc taacagcaa 
      agaattcga ggataaaat cgaaattcc cacaatttt 
      caaagctgg gttttcgct acgagcgtc cgcgagtgt 
      cgagttatt aatttgcga tggttggaa cccttacaa 
      ccacccttg ctaccactt aaacatgag tcaaaatgc 
      gatttttct acggaaata tatgacttt gccgaagaa 
      tatttcagt gagcgataa actgttaga ttcgtatca 
      ggcgttcgc taagatttt atccggtga tgagtggcg 
      taatttcgt gataagtct ggacgataa aggtgcttt 
      cagcttaac tgatgtcag tgttaacag aactgttat 
      ccaatacga agataagga agcgctaaa accgctcta 
      tttgctcgt gcgaagtgc tcaagttgc actgtggaa 
      aatacacca aaaccagtt tttttacac caaaacagt 
      acaaacgtc acgttttgg gcctttgtt gcacctccc 
      ttcgaaatt tctctgtgt gagtttgta cgtctcgac 
      ttgtcccac ctgttctat gactttttt tatcataaa 
      agacgggca aaagaaaat tttaaagga cgtgcagcc 
      cactcaaga ttttcagtg gcggctcgt gaaaatcaa 
      agacgataa tgttgaaag cgacaagct taatatttt 
      tgtggagac aaaatcggc aaacaatga tggcttttg 
      ggcggtcga tgatgtgag agtgtgtca cgagaacga 
      ggtgaaagt cacgttttt gaaactgga aaatgggct 
      gaaagagga cgaaactga tgaagtttc gtgtcaagt 
      ggttgagca cccgaaatg gaggcccaa gaagttggt 
      ttattccga aatcaaaga ttttccaac agtagataa 
      ttcgtcaaa ttcatcatc gatttactt aaactggac 
      tcaggaaga ccccaacaa ccaaataaa ctatttcat 
      cacatacga ggccaacga tttgtgccc tcctgagaa 
      tcctaactt agaaagagt aaaatcgaa cggttttct 
      aatgacaaa acataatga aataacaat aaacgacct 
      cattcccta caccagata caacatcac gttctcgag 
      catgctcca cgagaagga gaaacgaat ttttgtgac 
      aactctaga atcagccca attatcact cgaagaaat 
      tccttcaaa actacaaat cgaagtgca cgacaccca 
      agcgtacgc atcttggga caaaatcgc ctgtaaatc 
      aatttacgg cccggtttt atatgcact taatttaac 
      gaatgttaa gacaaacat taccaaaga attgggagt 
      gcaaactga agatacggt cgagatcaa cgatttgca 
      accttttac cacgtacat gcttgaaat tacaacagc 
      acgtaactt tgaaaacat acaaaataa caaaaacac 
      gctcaaaat cgagaacaa gttcacgac acagccagg 
      gaaggcacc acctgttga aaatttgga aatttactc 
      gattggtaa aaatacagt ctcgcttcg ttacgactt 
      gcccaggaa ttcgcgagg ggttccagt gtacgggca 
      agtgactcg ttgcaatgc tcttacgtt caaaaaatg 
      caaaacttc gaattttcc cataacgcc gtgtaaatt 
      atggccgag gaaatactg tatcgagat gaacaattt 
      gattccgta tcaaaacta cacgtttaa agttagtat 
      caggaacga aaggacgca gttttatgg tgatgatgt 
      gttggttga ggcgttttt ggtggaaca agttccggg 
      gtcgccgtc caatataac ttacaaaat cgttgactg 
      taaggaaag tttggacta ttgtcattt gaacgtgag 
      ctggatgca cccctacaa ccaaaatgg ttcaatcga 
      atcgtttca tattgtact gaatggtac agataatga 
      tacagcaaa cagtattca agaagtttt gaaaatatt 
      caactcgtc ctactacgt gccacgata tgaatacca 
      gatcaagta catacccta tagcactca ctacgaact 
      attcgtcca atcagcaaa ctcaaaata caagagccc 
      ttttgccca cgtccacgt caaaacgga cgatttggg 
      cgaacacat tgaccaaaa ccctatctt actcaaatc 
      cgagcccac ttcagtaac gttcacttt acccaccct 
      tgaccaccg tttgaaatc ctacagtct tgtcgtaat 
      tgtccaaga cttcaacca aaacatcac aattccgac 
      aaaaataac gaaaaatag caaaatcag cgaccactt 
      gtgccacga ctttggcga cacttggat tctacaaaa 
      actggaagt agccacaca taatcaggc caaaaattt 
      ggaaattgg aactgataa gcctttaaa acccaacac 
      tcaatattg cttcacttt catcgtcac caacaaata 
      cagaaatag cgaacacga tgtggttta ttacaagaa 
      gttgacgac gccacaaag cgaaatttc ggaaagtgg 
      ttcgaacgg cctctacgc tctattttt gctactttt 
      gctaatacc gatcgggtt tttcgccta taggtattt 
      caaaaagag aacacctag aaggaagcg tcaacagga 
      gaacaataa ggaaaacgt ttacgaatc cttgccgtt 
      tgaagaaac cgaagaaaa ctgcgtcag taacgacac 
      ttacgacca cttgttaca taaatagtt tttaacgta 
      gttgtactt aaatgtgtt ctgtgataa caaacgtct 
      cgtgtatat ttttactaa caaaactgt aattttaat 
      gtgccatac taagtgttc aagtgccca caaaccaat 
      tttgtaata cttaataaa ccataatat ccgag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY