Status
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Gene information
General information
TC009337 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 501575..521425
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009337
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01530

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009337 RA
      MEQVRLLVQ EQGREARNL LTFNISAFN DTSFGSKVS 
      RKPPCSPRA SESQQSGKL SLGVRGRTG ARVRSASTG 
      RDKRSELRA RYWALLFGN LQRSIAEIY NTVETHESI 
      TECQEVLLV LENYLRDFN ALADWFRLK WDYENTPPL 
      QRPTSLTWD ICKTNLSKS NNRSGKSSP NLASGRNSP 
      NVSGKISPR ILTNKIKNS ASAPTSPLP TIDQPIIEG 
      KVIETLASP KSVDTQTSP EYLEEAKVE TKAVESQTE 
      QLVKSPLTK RKTEIKVSE VKPKPVPKP KDAKSNVPL 
      VKKETKTVK PKIDTNNKP KTQFKKSTE DVTQEDTNS 
      NQTAEKSTN TEDFPKLPP VKKPAKINQ ESQTEEIEK 
      KLNKTTKIE TVKTTKPVT TTTRPAYST ALTKSFSAK 
      AVPTVKPKV ETRPVVKPA VRLTKPVVS ARTANPTRS 
      GLARSKTVG DMKSVKLEQ RSKIAPKVA NTSKFNKTT 
      KPPVLTKSK TTLTREHLN KSMSFNDYA SSVETLVNQ 
      GTIVTNSSN SIASSSETL NNDNKNDQL SDGWLTVKT 
      RSRFKNSNN RSRRSDTAL TWATRFHQV SATASLPAL 
      ALLPENNEV NKTPKCIDK SVKENLNTF KSLKDQGRT 
      PLRRSHTTL SRMTVSKSS VSEKNKTNL HIKKTAEER 
      RMKVSDIDS ETDDETKFK DLQEDSATE EEHRKKAKQ 
      LSEEEDRLT KEIEQLKCL EIEVDTETE TDGELQGDS 
      DDVPIEPTN EDDYSMSLE ARYEPMLAG MSWSDRIDT 
      LAALEDLNA RHPGRALVL HQKLSNPAR KVSLAEALR 
      KYQVKQARA EQRRQELQQ EKAQKLHAL LCRVEDVKA 
      AKLQLIEDK RKRMESRMQ KATQNRRRY IKDIIKKAH 
      DEEEKLKEI AFINELEAQ NKRHDFLQS CREQRGRIQ 
      CIKEDRRKR LEEKAAKEA AVEERRKAL ERERKERLD 
      RLKDERRQR DERIGQQQQ QRERERQEL AREKARDRE 
      ERLSALHAQ QLASTQELQ KRIEQKQEE SKRRHEENM 
      EQIRQKALE LSIHRCHNE DNQAPNSTP YPTQKFCTT 
      CNTLIKSEV YLMSHLRGR AHQEAVKQA NPGQTILTT 
      NELEQYNLK QIVDAPAGK EDPKDVAAK ERGRAHRKR 
      CKKIRQRMV MKGAEFETS YKPTNVECA NKRSLNRNI 
      NTLGSITNQ ASQGLSPTS SSQLDRILN ELNRSLSKG 
      SDNDLIVFQ NVGGFTVLG KLLSMGQDT TISSISTKT 
      LITCCNLWQ IVCKGSGSG SANCEYIVM SNRLTPAID 
      LLNTKLQGL GCSEDLPPT DSLCTALMQ LLATVLRNT 
      PKSVPAHRF HDIVSFCVC VGIVEQLAQ CCLAVRGPV 
      HDVPPACVF LLAALEFLA ALADCCPED ADPTHLVST 
      LHGTELLGA VSMLYGTLL PPDSSPRVE GQSPPIIPD 
      PCLYLATAT FRLLRRIAE LDLIKFQEA LGAEGISLQ 
      FRHIASHLI WCCALPTTS PRSKDNSEI HQELLHEVI 
      AVTGYFAVE NNENQMLLV SGQPPSVLQ QLCSLPFPY 
      FSVEGLSNV LYPTLLACC VRNEHTTAI LKQELSYDI 
      LEDFRKSTT GQQHRLVKL LNKNHA
    CDS sequence
    • >TC009337 RA
      atggaacaa gtgcgatta ttagtacag gaacaagga 
      cgtgaagct aggaatctc ctcaccttt aacatttct 
      gctttcaac gatactagc tttggaagc aaagtctca 
      cggaagccc ccgtgttca ccaagggct tccgagtcg 
      caacaaagc ggaaaactc agtcttgga gtgcggggg 
      cgaacgggg gctagagtg cgctctgcc tccactgga 
      agggacaag aggtcggag ctgagggcc agatactgg 
      gccttgctg tttgggaat ttgcaaaga tctatcgct 
      gaaatttac aatacggtg gaaacacat gaaagtatt 
      accgaatgc caagaagta ttattagtt ttagagaat 
      tacttgcgt gattttaac gcactagcg gattggttt 
      cgcctaaaa tgggattat gaaaatact ccaccttta 
      caaagacca acatctctc acttgggac atttgtaaa 
      acgaattta tcgaaaagt aataacaga tctggtaaa 
      tcgagtcct aatctcgct tctggacgt aatagtcct 
      aacgtttct ggaaaaatt agtcctcga attttaacg 
      aataaaatt aaaaattcg gcttcggct cccacaagt 
      ccacttcct acaatcgac caaccaata atcgaagga 
      aaagttata gaaacactt gcatcacct aaaagtgtg 
      gacactcaa acgtcaccc gaatatttg gaagaagcg 
      aaagtcgag acaaaagcc gttgaaagt cagaccgaa 
      caattggtt aaatctcca ctgactaaa cgaaaaaca 
      gaaatcaag gtttcggag gttaaacca aaacctgtt 
      cctaaacca aaagacgca aaatcgaat gttccattg 
      gttaaaaag gaaactaaa accgtcaaa cctaaaatt 
      gacacaaac aacaaaccg aaaacccaa ttcaagaaa 
      agtacagaa gatgtaaca caagaagac acaaactcg 
      aatcaaaca gctgagaaa agtaccaat actgaagac 
      tttcccaag ttaccacca gttaaaaaa ccggccaaa 
      atcaaccaa gaatctcaa actgaagaa atcgagaag 
      aaacttaac aaaactact aaaatcgaa acggttaaa 
      actacgaaa cctgtcacc acaaccaca cggcctgct 
      tattcaact gctttaacc aaaagtttt tccgcaaag 
      gctgtacca actgttaaa cccaaagtt gagactaga 
      cctgtggtt aaaccagcg gttcggtta actaaaccg 
      gtggttagt gctcgcact gctaatcca actagaagt 
      ggtttagca cgtagcaaa accgttggc gatatgaag 
      agtgtgaaa ctagaacag agatcgaaa attgcacca 
      aaagtggca aatacgtcc aagtttaac aaaacaacc 
      aaaccgccg gttttaacc aaaagcaaa accacctta 
      actagagaa catttgaac aaatctatg tcgtttaat 
      gactatgcc agttctgta gaaactctc gtaaatcaa 
      gggactatt gttacaaat tcaagtaat agcattgcg 
      agttcttcg gaaactctt aataatgat aataaaaat 
      gatcaattg tctgacggt tggttgact gttaaaaca 
      cgttcaaga ttcaaaaac agtaataat cggtcaaga 
      cgttccgat actgcccta acttgggca acaaggttt 
      catcaagtt agcgcgact gcaagtcta ccagcttta 
      gccctgttg cctgaaaat aacgaagtg aataaaaca 
      cccaaatgt atcgataaa agtgtcaaa gaaaatctc 
      aacactttc aagtctttg aaggatcaa gggaggact 
      ccgttacgg cgttcgcac acaacattg agtcgaatg 
      acagtgtcg aaaagttcc gtttctgag aaaaacaag 
      acgaattta catatcaag aaaacagcg gaagaaaga 
      cgcatgaaa gtgtccgat attgattcg gaaacggac 
      gatgaaaca aagttcaaa gaccttcag gaggattca 
      gccactgaa gaagaacac cggaagaaa gcgaaacaa 
      ttatcggag gaagaggat cgtctgact aaagaaatt 
      gagcagctg aagtgtctg gaaattgaa gttgataca 
      gagacggaa accgatggc gaattgcaa ggtgatagt 
      gacgatgtc ccgattgaa ccaaccaat gaagacgac 
      tattccatg tctttggaa gctcgctat gaaccaatg 
      ttggctgga atgtcttgg agtgaccgc attgatact 
      ttagccgct ttggaagac ttgaatgcc agacatcca 
      ggacgtgct ctagtgtta caccagaaa ttatcaaac 
      cctgcacgt aaagtaagc cttgctgaa gctttacgc 
      aagtatcaa gtgaagcaa gcacgtgct gaacaacga 
      cgtcaagag ttacaacag gagaaggca caaaaactt 
      cacgcactt ttgtgtcgt gtcgaagac gtcaaagcc 
      gccaaattg caattaatc gaagacaag agaaaacga 
      atggaatct cggatgcag aaagcgacg caaaatcgc 
      cgccgttac atcaaggac atcatcaag aaagcacac 
      gatgaagaa gagaagttg aaagaaatc gcattcatt 
      aacgaactc gaagcgcaa aacaaacga cacgatttt 
      ctccaatcg tgtcgtgag caacgtggt cgcatccaa 
      tgcattaag gaagataga cgtaaacga ttggaggag 
      aaagcagca aaggaagca gctgtggag gaacgacgt 
      aaagcacta gaacgggaa cgtaaagag agattggat 
      cgtttgaag gacgagaga aggcaaagg gatgaaaga 
      atcggacag caacaacaa caaagggag agggaacga 
      caggagttg gcaagggag aaagcgaga gatcgtgag 
      gaacgctta tcagcgctt catgcacaa caattggct 
      tcgactcaa gagttgcaa aaaaggatc gaacagaag 
      caggaagag tcgaagaga cgacatgag gaaaatatg 
      gaacagatc aggcaaaag gcgttggag ttgtccatc 
      catcggtgt cataatgag gataatcag gcgccgaat 
      agtacgccc tatcccacc cagaagttt tgtacaacg 
      tgtaacact ttgatcaaa tcagaggtt tacttaatg 
      agccatctc cgtggccgc gcgcaccag gaggccgta 
      aaacaggca aatcctggc cagactatt ctaacaacc 
      aatgaatta gagcagtac aatttgaaa caaattgtt 
      gacgcaccg gccggtaaa gaagatccg aaagatgtg 
      gcggctaaa gaacgtggt agagcccat cgtaaacga 
      tgtaaaaaa attagacaa cgcatggta atgaaaggt 
      gcagaattt gaaacttcg tacaaaccg accaatgtc 
      gaatgtgct aataaacga agtttaaat cggaatatt 
      aatactctc ggtagtatt acaaatcaa gctagtcaa 
      gggttgtct ccgaccagt tctagtcaa ttggatcga 
      atcctcaac gaacttaat agatcgttg agtaaaggg 
      agtgataat gatttgatt gtgtttcaa aatgttggc 
      gggtttacc gttttgggt aaattgctt tcgatgggt 
      caagatacc acgatttcg tccatttcg accaagact 
      ttgattacg tgttgcaat ttgtggcaa attgtctgt 
      aaaggatca gggagtgga tcagctaat tgcgaatac 
      attgttatg tccaatcgg ctcacaccc gccattgat 
      ctactcaac actaaatta cagggtttg ggttgttca 
      gaggatttg ccaccgact gatagtctt tgtacggct 
      ttgatgcag cttttggcc acggttttg agaaatacg 
      ccgaaaagt gtcccagca catcggttt cacgacata 
      gtaagcttt tgcgtgtgc gtcggaatc gtcgaacaa 
      ttggcccaa tgctgtttg gcggtgaga ggccccgtc 
      catgacgtc cctccagct tgtgtcttc ctccttgct 
      gccttggag tttctagct gctcttgct gattgctgt 
      ccagaggat gcagatccc acacattta gtaagtact 
      ttacacggt actgaacta ttgggggcc gtgtcaatg 
      ctgtacggc acattgcta cccccagat tcttcccca 
      cgtgtcgaa ggccaatct cctcccatc attcctgac 
      ccttgtctc tatttggcc actgcaact ttccgactt 
      cttcgtcga atcgccgaa ttggactta attaagttt 
      caggaagca cttggtgcc gagggtatt tccttgcag 
      tttcgtcat attgcaagt cacttaatt tggtgctgt 
      gcacttccg acaacgtct ccaagaagc aaagacaac 
      agtgagatt catcaagag ctgcttcac gaagtgatt 
      gccgttact ggttacttt gctgtggaa aataatgag 
      aatcagatg ttgctagtg agtgggcaa cctccttct 
      gttctgcaa cagctttgt tcgttgcca tttccttat 
      ttctcagtg gaggggctt tcgaacgtt ctgtatcca 
      actttgctg gcttgctgc gttcgaaac gaacacacc 
      actgcaatt ctaaagcag gaactttcc tacgatatt 
      ttggaggat tttcggaaa tcaacaaca gggcaacaa 
      catcggctg gttaaactt ttaaataaa aatcacgct 
      taa
    mRNA sequence
    • >TC009337 RA
      tttttgatt agttttttt tgccctatt gccataaat 
      caaaaccag agtacttaa catcagaag aaccaatat 
      tttttgaaa gcgaaaagt gggaaaatt gttccctat 
      ttgtcttgg tggagaaat ctagacttg atcagctgt 
      ttgaaagaa agagcatgg cgattgttg tgaaaccgc 
      tgtcaaaaa ttcaaacat caatcacaa gaagtgatg 
      taaaaaatc tgtaaaaac atccaaaaa aagcaaatc 
      atggaacaa gtgcgatta ttagtacag gaacaagga 
      cgtgaagct aggaatctc ctcaccttt aacatttct 
      gctttcaac gatactagc tttggaagc aaagtctca 
      cggaagccc ccgtgttca ccaagggct tccgagtcg 
      caacaaagc ggaaaactc agtcttgga gtgcggggg 
      cgaacgggg gctagagtg cgctctgcc tccactgga 
      agggacaag aggtcggag ctgagggcc agatactgg 
      gccttgctg tttgggaat ttgcaaaga tctatcgct 
      gaaatttac aatacggtg gaaacacat gaaagtatt 
      accgaatgc caagaagta ttattagtt ttagagaat 
      tacttgcgt gattttaac gcactagcg gattggttt 
      cgcctaaaa tgggattat gaaaatact ccaccttta 
      caaagacca acatctctc acttgggac atttgtaaa 
      acgaattta tcgaaaagt aataacaga tctggtaaa 
      tcgagtcct aatctcgct tctggacgt aatagtcct 
      aacgtttct ggaaaaatt agtcctcga attttaacg 
      aataaaatt aaaaattcg gcttcggct cccacaagt 
      ccacttcct acaatcgac caaccaata atcgaagga 
      aaagttata gaaacactt gcatcacct aaaagtgtg 
      gacactcaa acgtcaccc gaatatttg gaagaagcg 
      aaagtcgag acaaaagcc gttgaaagt cagaccgaa 
      caattggtt aaatctcca ctgactaaa cgaaaaaca 
      gaaatcaag gtttcggag gttaaacca aaacctgtt 
      cctaaacca aaagacgca aaatcgaat gttccattg 
      gttaaaaag gaaactaaa accgtcaaa cctaaaatt 
      gacacaaac aacaaaccg aaaacccaa ttcaagaaa 
      agtacagaa gatgtaaca caagaagac acaaactcg 
      aatcaaaca gctgagaaa agtaccaat actgaagac 
      tttcccaag ttaccacca gttaaaaaa ccggccaaa 
      atcaaccaa gaatctcaa actgaagaa atcgagaag 
      aaacttaac aaaactact aaaatcgaa acggttaaa 
      actacgaaa cctgtcacc acaaccaca cggcctgct 
      tattcaact gctttaacc aaaagtttt tccgcaaag 
      gctgtacca actgttaaa cccaaagtt gagactaga 
      cctgtggtt aaaccagcg gttcggtta actaaaccg 
      gtggttagt gctcgcact gctaatcca actagaagt 
      ggtttagca cgtagcaaa accgttggc gatatgaag 
      agtgtgaaa ctagaacag agatcgaaa attgcacca 
      aaagtggca aatacgtcc aagtttaac aaaacaacc 
      aaaccgccg gttttaacc aaaagcaaa accacctta 
      actagagaa catttgaac aaatctatg tcgtttaat 
      gactatgcc agttctgta gaaactctc gtaaatcaa 
      gggactatt gttacaaat tcaagtaat agcattgcg 
      agttcttcg gaaactctt aataatgat aataaaaat 
      gatcaattg tctgacggt tggttgact gttaaaaca 
      cgttcaaga ttcaaaaac agtaataat cggtcaaga 
      cgttccgat actgcccta acttgggca acaaggttt 
      catcaagtt agcgcgact gcaagtcta ccagcttta 
      gccctgttg cctgaaaat aacgaagtg aataaaaca 
      cccaaatgt atcgataaa agtgtcaaa gaaaatctc 
      aacactttc aagtctttg aaggatcaa gggaggact 
      ccgttacgg cgttcgcac acaacattg agtcgaatg 
      acagtgtcg aaaagttcc gtttctgag aaaaacaag 
      acgaattta catatcaag aaaacagcg gaagaaaga 
      cgcatgaaa gtgtccgat attgattcg gaaacggac 
      gatgaaaca aagttcaaa gaccttcag gaggattca 
      gccactgaa gaagaacac cggaagaaa gcgaaacaa 
      ttatcggag gaagaggat cgtctgact aaagaaatt 
      gagcagctg aagtgtctg gaaattgaa gttgataca 
      gagacggaa accgatggc gaattgcaa ggtgatagt 
      gacgatgtc ccgattgaa ccaaccaat gaagacgac 
      tattccatg tctttggaa gctcgctat gaaccaatg 
      ttggctgga atgtcttgg agtgaccgc attgatact 
      ttagccgct ttggaagac ttgaatgcc agacatcca 
      ggacgtgct ctagtgtta caccagaaa ttatcaaac 
      cctgcacgt aaagtaagc cttgctgaa gctttacgc 
      aagtatcaa gtgaagcaa gcacgtgct gaacaacga 
      cgtcaagag ttacaacag gagaaggca caaaaactt 
      cacgcactt ttgtgtcgt gtcgaagac gtcaaagcc 
      gccaaattg caattaatc gaagacaag agaaaacga 
      atggaatct cggatgcag aaagcgacg caaaatcgc 
      cgccgttac atcaaggac atcatcaag aaagcacac 
      gatgaagaa gagaagttg aaagaaatc gcattcatt 
      aacgaactc gaagcgcaa aacaaacga cacgatttt 
      ctccaatcg tgtcgtgag caacgtggt cgcatccaa 
      tgcattaag gaagataga cgtaaacga ttggaggag 
      aaagcagca aaggaagca gctgtggag gaacgacgt 
      aaagcacta gaacgggaa cgtaaagag agattggat 
      cgtttgaag gacgagaga aggcaaagg gatgaaaga 
      atcggacag caacaacaa caaagggag agggaacga 
      caggagttg gcaagggag aaagcgaga gatcgtgag 
      gaacgctta tcagcgctt catgcacaa caattggct 
      tcgactcaa gagttgcaa aaaaggatc gaacagaag 
      caggaagag tcgaagaga cgacatgag gaaaatatg 
      gaacagatc aggcaaaag gcgttggag ttgtccatc 
      catcggtgt cataatgag gataatcag gcgccgaat 
      agtacgccc tatcccacc cagaagttt tgtacaacg 
      tgtaacact ttgatcaaa tcagaggtt tacttaatg 
      agccatctc cgtggccgc gcgcaccag gaggccgta 
      aaacaggca aatcctggc cagactatt ctaacaacc 
      aatgaatta gagcagtac aatttgaaa caaattgtt 
      gacgcaccg gccggtaaa gaagatccg aaagatgtg 
      gcggctaaa gaacgtggt agagcccat cgtaaacga 
      tgtaaaaaa attagacaa cgcatggta atgaaaggt 
      gcagaattt gaaacttcg tacaaaccg accaatgtc 
      gaatgtgct aataaacga agtttaaat cggaatatt 
      aatactctc ggtagtatt acaaatcaa gctagtcaa 
      gggttgtct ccgaccagt tctagtcaa ttggatcga 
      atcctcaac gaacttaat agatcgttg agtaaaggg 
      agtgataat gatttgatt gtgtttcaa aatgttggc 
      gggtttacc gttttgggt aaattgctt tcgatgggt 
      caagatacc acgatttcg tccatttcg accaagact 
      ttgattacg tgttgcaat ttgtggcaa attgtctgt 
      aaaggatca gggagtgga tcagctaat tgcgaatac 
      attgttatg tccaatcgg ctcacaccc gccattgat 
      ctactcaac actaaatta cagggtttg ggttgttca 
      gaggatttg ccaccgact gatagtctt tgtacggct 
      ttgatgcag cttttggcc acggttttg agaaatacg 
      ccgaaaagt gtcccagca catcggttt cacgacata 
      gtaagcttt tgcgtgtgc gtcggaatc gtcgaacaa 
      ttggcccaa tgctgtttg gcggtgaga ggccccgtc 
      catgacgtc cctccagct tgtgtcttc ctccttgct 
      gccttggag tttctagct gctcttgct gattgctgt 
      ccagaggat gcagatccc acacattta gtaagtact 
      ttacacggt actgaacta ttgggggcc gtgtcaatg 
      ctgtacggc acattgcta cccccagat tcttcccca 
      cgtgtcgaa ggccaatct cctcccatc attcctgac 
      ccttgtctc tatttggcc actgcaact ttccgactt 
      cttcgtcga atcgccgaa ttggactta attaagttt 
      caggaagca cttggtgcc gagggtatt tccttgcag 
      tttcgtcat attgcaagt cacttaatt tggtgctgt 
      gcacttccg acaacgtct ccaagaagc aaagacaac 
      agtgagatt catcaagag ctgcttcac gaagtgatt 
      gccgttact ggttacttt gctgtggaa aataatgag 
      aatcagatg ttgctagtg agtgggcaa cctccttct 
      gttctgcaa cagctttgt tcgttgcca tttccttat 
      ttctcagtg gaggggctt tcgaacgtt ctgtatcca 
      actttgctg gcttgctgc gttcgaaac gaacacacc 
      actgcaatt ctaaagcag gaactttcc tacgatatt 
      ttggaggat tttcggaaa tcaacaaca gggcaacaa 
      catcggctg gttaaactt ttaaataaa aatcacgct 
      taaaagttg taacgtgca atttaatta agtcgattc 
      cattagtca ttaaactaa cttatgtct aacagcgta 
      ctgattttt aatataatg tggcactta aaaatcttc 
      actttgttt agttttgat gtgatgtaa cgttttgag 
      agattatta tgtgatcgt gatggtaat taattaaca 
      cactttaat tatgtgtag cttttctat ataataaag 
      tagcgagag aaat
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