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Gene information
General information
TC009331 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 464275..467679
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009331
    iBeetle screen iB_01529

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009331 RA
      MNKNNRSWF KKKPSQINL NLVNVATNF NNEPKYGYY 
GFKLYFPES TDDETKDET LLKYVLHFE NFIKRHSAN 
FSLGEIEAQ KCFVIDYKL ITSDEELSA GWKGLETEI 
ITNTDLCLS CMGLAMHQF IMKDYDNNS RKKSLGIIR 
ARLINYEPI QQIKDIRVN NYGRLISLK GTVIKAANV 
KIMYQYMAF SCATCTGIQ VVKQPDNIF TVPNKCLTE 
GCKARSNFQ ALHSSPFTR TISWQHIKI QELIGNDEF 
ENGRVPRTL ECELTEDLV NSCVPGDDV TITGVIKVR 
NNAETSYKN KEKSVFLLY LDVVSVVNN KNQNEGTYG 
ASERITFNI TDYYAIQKI HAEPYLFRF LVQSLCPTI 
YGHEIVKAG LLLALFGGT KSSKFRAES HVLMVGDPG 
IGKSQMLHA CVNVAPRGV YVCGNTSTG SGLTVTMTR 
EAKGEYSLE AGALMLADQ GCCCIDEFD KMPTQHACL 
LEVMEQQSI SIAKAGIVC TLPTRATIL AAANPAGGH 
YNKAKTIAE NLKISSPML SRFDLVFIL LDQPNEDLD 
MRLSEHILA LHSRRNGSN VSKNSTLAE GVNNSLRGR 
LSLQDGEEI DYLPHSLFR KYIAYAQKY VNPQLSDDA 
KQVLKDFYF QLRKEFQNG DSTPVTTRQ LNSLMRLTQ 
ARAKAELRE EATKEDAQD VVEIMRQTL IDIFTDNVG 
ILDTTRSQN GAGMSGKNQ VVKLLRLMQ QKAEEQTKS 
IFTTNEIRN LSQDVGINP SKFFSVLQS LNIQGFILN 
KGANRYQLV TADL
    CDS sequence
    • >TC009331 RA
      atgaataaa aataaccgt tcctggttt aagaagaaa 
ccttctcaa attaactta aaccttgtc aatgtcgca 
accaacttt aacaacgaa ccgaaatat ggttattac 
ggatttaaa ctgtacttc cctgaatca acagacgac 
gaaaccaaa gatgagact ctccttaaa tacgtgctt 
cattttgaa aatttcatt aaacggcat agtgccaat 
ttttcgctt ggcgaaatc gaggcccaa aaatgcttt 
gtgattgac tataagctc ataacttca gacgaagaa 
ttaagtgcg ggatggaaa ggtttagaa acggagata 
atcacaaac actgatctt tgcctaagt tgtatgggt 
ttagccatg caccagttt atcatgaag gattacgat 
aacaacagt aggaaaaaa agtttaggg ataattaga 
gcgagactt atcaattac gagcccatt caacaaata 
aaagacata cgggtcaat aattatggt cgtttaatt 
tcgctcaaa ggcactgtg ataaaagcc gctaatgtt 
aaaattatg taccaatat atggcattt tcatgtgca 
acttgtaca ggaattcaa gtcgtcaaa caaccagat 
aacattttt acagtacct aacaaatgt ttaacggaa 
gggtgcaaa gcccgctct aatttccaa gccttgcat 
tcgtcccct ttcacacga acgattagc tggcaacac 
atcaaaatt caagaattg attggaaac gatgagttt 
gaaaacggg cgcgttccc agaactttg gaatgcgaa 
ttgactgaa gatttggtc aattcttgt gtcccggga 
gatgatgtc acgataaca ggcgttata aaagtgcgt 
aataatgcc gaaacttcg tataaaaat aaggagaaa 
agtgttttt ctcctttat ttggacgtt gtttcagtt 
gtaaataac aaaaatcag aatgagggt acttatggg 
gcttcggaa cggataact ttcaatatt actgactat 
tacgcaatt caaaaaatt catgccgag ccttatctc 
tttcgtttc cttgtccaa tcactatgc cccacaatt 
tacggccac gagatcgtc aaagcaggc cttcttctt 
gcccttttt ggcggcaca aaatcgtcg aaatttcgt 
gccgaatcg cacgtttta atggttggc gaccctgga 
atcggtaaa tcccagatg ttgcatgct tgtgtaaac 
gtagccccc agaggtgtg tacgtttgt ggtaacaca 
agtactgga tctggatta actgttaca atgactcgg 
gaagctaaa ggagaatat tccctagaa gcaggggcg 
ctaatgttg gccgatcag gggtgttgt tgtatagac 
gagttcgat aaaatgccc acacaacat gcttgtctc 
ttagaagta atggagcag caaagtatt agtatcgca 
aaggctggg attgtatgt actttacca acacgtgct 
acgatttta gcggctgct aatccagct ggggggcat 
tataataaa gctaaaact atagctgaa aatttgaaa 
attagttcg ccgatgttg tcacgattt gatttggtt 
tttatactt ctggatcaa ccaaatgag gatttggat 
atgaggcta tctgaacat attctagct ttgcattca 
aggcgaaat ggatcaaat gtgtcaaaa aattcaaca 
ttggccgaa ggtgtaaac aactccttg cgaggacgc 
ttgtctctt caagacggg gaggaaata gactatttg 
ccgcactcc ttatttcga aaatacata gcttatgcg 
caaaaatat gtgaatccc caactttca gatgacgct 
aaacaagta ttgaaagat ttttatttc caactccga 
aaggagttt caaaatggg gatagtacc cctgttacg 
accagacaa ctaaattct ttaatgaga ctgactcag 
gctagagca aaagccgaa ctgagagaa gaagcaact 
aaagaggac gctcaagac gttgtggaa attatgcga 
caaacttta atagacatt tttaccgat aatgtcgga 
attttagat accactcgg tcgcaaaat ggggccgga 
atgagcggc aaaaatcaa gtcgttaag ttgctaaga 
ctcatgcag caaaaggca gaagaacaa accaaatcg 
atttttaca acaaacgaa atccgaaat ttatcccaa 
gatgtggga attaaccct tccaagttt ttctctgtc 
ttgcaaagt ttaaacatt caaggtttt attttgaat 
aaaggcgcc aatcgatat caacttgtc actgccgat 
ttgtaa
    mRNA sequence
    • >TC009331 RA
      cctcacttt ttcaaacaa tttgttttt tatggtgaa 
ataattaaa tatgaataa aaataaccg ttcctggtt 
taagaagaa accttctca aattaactt aaaccttgt 
caatgtcgc aaccaactt taacaacga accgaaata 
tggttatta cggatttaa actgtactt ccctgaatc 
aacagacga cgaaaccaa agatgagac tctccttaa 
atacgtgct tcattttga aaatttcat taaacggca 
tagtgccaa tttttcgct tggcgaaat cgaggccca 
aaaatgctt tgtgattga ctataagct cataacttc 
agacgaaga attaagtgc gggatggaa aggtttaga 
aacggagat aatcacaaa cactgatct ttgcctaag 
ttgtatggg tttagccat gcaccagtt tatcatgaa 
ggattacga taacaacag taggaaaaa aagtttagg 
gataattag agcgagact tatcaatta cgagcccat 
tcaacaaat aaaagacat acgggtcaa taattatgg 
tcgtttaat ttcgctcaa aggcactgt gataaaagc 
cgctaatgt taaaattat gtaccaata tatggcatt 
ttcatgtgc aacttgtac aggaattca agtcgtcaa 
acaaccaga taacatttt tacagtacc taacaaatg 
tttaacgga agggtgcaa agcccgctc taatttcca 
agccttgca ttcgtcccc tttcacacg aacgattag 
ctggcaaca catcaaaat tcaagaatt gattggaaa 
cgatgagtt tgaaaacgg gcgcgttcc cagaacttt 
ggaatgcga attgactga agatttggt caattcttg 
tgtcccggg agatgatgt cacgataac aggcgttat 
aaaagtgcg taataatgc cgaaacttc gtataaaaa 
taaggagaa aagtgtttt tctccttta tttggacgt 
tgtttcagt tgtaaataa caaaaatca gaatgaggg 
tacttatgg ggcttcgga acggataac tttcaatat 
tactgacta ttacgcaat tcaaaaaat tcatgccga 
gccttatct ctttcgttt ccttgtcca atcactatg 
ccccacaat ttacggcca cgagatcgt caaagcagg 
ccttcttct tgccctttt tggcggcac aaaatcgtc 
gaaatttcg tgccgaatc gcacgtttt aatggttgg 
cgaccctgg aatcggtaa atcccagat gttgcatgc 
ttgtgtaaa cgtagcccc cagaggtgt gtacgtttg 
tggtaacac aagtactgg atctggatt aactgttac 
aatgactcg ggaagctaa aggagaata ttccctaga 
agcaggggc gctaatgtt ggccgatca ggggtgttg 
ttgtataga cgagttcga taaaatgcc cacacaaca 
tgcttgtct cttagaagt aatggagca gcaaagtat 
tagtatcgc aaaggctgg gattgtatg tactttacc 
aacacgtgc tacgatttt agcggctgc taatccagc 
tggggggca ttataataa agctaaaac tatagctga 
aaatttgaa aattagttc gccgatgtt gtcacgatt 
tgatttggt ttttatact tctggatca accaaatga 
ggatttgga tatgaggct atctgaaca tattctagc 
tttgcattc aaggcgaaa tggatcaaa tgtgtcaaa 
aaattcaac attggccga aggtgtaaa caactcctt 
gcgaggacg cttgtctct tcaagacgg ggaggaaat 
agactattt gccgcactc cttatttcg aaaatacat 
agcttatgc gcaaaaata tgtgaatcc ccaactttc 
agatgacgc taaacaagt attgaaaga tttttattt 
ccaactccg aaaggagtt tcaaaatgg ggatagtac 
ccctgttac gaccagaca actaaattc tttaatgag 
actgactca ggctagagc aaaagccga actgagaga 
agaagcaac taaagagga cgctcaaga cgttgtgga 
aattatgcg acaaacttt aatagacat ttttaccga 
taatgtcgg aattttaga taccactcg gtcgcaaaa 
tggggccgg aatgagcgg caaaaatca agtcgttaa 
gttgctaag actcatgca gcaaaaggc agaagaaca 
aaccaaatc gatttttac aacaaacga aatccgaaa 
tttatccca agatgtggg aattaaccc ttccaagtt 
tttctctgt cttgcaaag tttaaacat tcaaggttt 
tattttgaa taaaggcgc caatcgata tcaacttgt 
cactgccga tttgtaatt atgtaattt tgtgaataa 
aactatctt tttgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY