| General information | |||
| TC009331 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 464275..467679 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC009331 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01529 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
remarks: bad quality of preparation,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC009331 RA
MNKNNRSWF KKKPSQINL NLVNVATNF NNEPKYGYY GFKLYFPES TDDETKDET LLKYVLHFE NFIKRHSAN FSLGEIEAQ KCFVIDYKL ITSDEELSA GWKGLETEI ITNTDLCLS CMGLAMHQF IMKDYDNNS RKKSLGIIR ARLINYEPI QQIKDIRVN NYGRLISLK GTVIKAANV KIMYQYMAF SCATCTGIQ VVKQPDNIF TVPNKCLTE GCKARSNFQ ALHSSPFTR TISWQHIKI QELIGNDEF ENGRVPRTL ECELTEDLV NSCVPGDDV TITGVIKVR NNAETSYKN KEKSVFLLY LDVVSVVNN KNQNEGTYG ASERITFNI TDYYAIQKI HAEPYLFRF LVQSLCPTI YGHEIVKAG LLLALFGGT KSSKFRAES HVLMVGDPG IGKSQMLHA CVNVAPRGV YVCGNTSTG SGLTVTMTR EAKGEYSLE AGALMLADQ GCCCIDEFD KMPTQHACL LEVMEQQSI SIAKAGIVC TLPTRATIL AAANPAGGH YNKAKTIAE NLKISSPML SRFDLVFIL LDQPNEDLD MRLSEHILA LHSRRNGSN VSKNSTLAE GVNNSLRGR LSLQDGEEI DYLPHSLFR KYIAYAQKY VNPQLSDDA KQVLKDFYF QLRKEFQNG DSTPVTTRQ LNSLMRLTQ ARAKAELRE EATKEDAQD VVEIMRQTL IDIFTDNVG ILDTTRSQN GAGMSGKNQ VVKLLRLMQ QKAEEQTKS IFTTNEIRN LSQDVGINP SKFFSVLQS LNIQGFILN KGANRYQLV TADL
- >TC009331 RA
atgaataaa aataaccgt tcctggttt aagaagaaa ccttctcaa attaactta aaccttgtc aatgtcgca accaacttt aacaacgaa ccgaaatat ggttattac ggatttaaa ctgtacttc cctgaatca acagacgac gaaaccaaa gatgagact ctccttaaa tacgtgctt cattttgaa aatttcatt aaacggcat agtgccaat ttttcgctt ggcgaaatc gaggcccaa aaatgcttt gtgattgac tataagctc ataacttca gacgaagaa ttaagtgcg ggatggaaa ggtttagaa acggagata atcacaaac actgatctt tgcctaagt tgtatgggt ttagccatg caccagttt atcatgaag gattacgat aacaacagt aggaaaaaa agtttaggg ataattaga gcgagactt atcaattac gagcccatt caacaaata aaagacata cgggtcaat aattatggt cgtttaatt tcgctcaaa ggcactgtg ataaaagcc gctaatgtt aaaattatg taccaatat atggcattt tcatgtgca acttgtaca ggaattcaa gtcgtcaaa caaccagat aacattttt acagtacct aacaaatgt ttaacggaa gggtgcaaa gcccgctct aatttccaa gccttgcat tcgtcccct ttcacacga acgattagc tggcaacac atcaaaatt caagaattg attggaaac gatgagttt gaaaacggg cgcgttccc agaactttg gaatgcgaa ttgactgaa gatttggtc aattcttgt gtcccggga gatgatgtc acgataaca ggcgttata aaagtgcgt aataatgcc gaaacttcg tataaaaat aaggagaaa agtgttttt ctcctttat ttggacgtt gtttcagtt gtaaataac aaaaatcag aatgagggt acttatggg gcttcggaa cggataact ttcaatatt actgactat tacgcaatt caaaaaatt catgccgag ccttatctc tttcgtttc cttgtccaa tcactatgc cccacaatt tacggccac gagatcgtc aaagcaggc cttcttctt gcccttttt ggcggcaca aaatcgtcg aaatttcgt gccgaatcg cacgtttta atggttggc gaccctgga atcggtaaa tcccagatg ttgcatgct tgtgtaaac gtagccccc agaggtgtg tacgtttgt ggtaacaca agtactgga tctggatta actgttaca atgactcgg gaagctaaa ggagaatat tccctagaa gcaggggcg ctaatgttg gccgatcag gggtgttgt tgtatagac gagttcgat aaaatgccc acacaacat gcttgtctc ttagaagta atggagcag caaagtatt agtatcgca aaggctggg attgtatgt actttacca acacgtgct acgatttta gcggctgct aatccagct ggggggcat tataataaa gctaaaact atagctgaa aatttgaaa attagttcg ccgatgttg tcacgattt gatttggtt tttatactt ctggatcaa ccaaatgag gatttggat atgaggcta tctgaacat attctagct ttgcattca aggcgaaat ggatcaaat gtgtcaaaa aattcaaca ttggccgaa ggtgtaaac aactccttg cgaggacgc ttgtctctt caagacggg gaggaaata gactatttg ccgcactcc ttatttcga aaatacata gcttatgcg caaaaatat gtgaatccc caactttca gatgacgct aaacaagta ttgaaagat ttttatttc caactccga aaggagttt caaaatggg gatagtacc cctgttacg accagacaa ctaaattct ttaatgaga ctgactcag gctagagca aaagccgaa ctgagagaa gaagcaact aaagaggac gctcaagac gttgtggaa attatgcga caaacttta atagacatt tttaccgat aatgtcgga attttagat accactcgg tcgcaaaat ggggccgga atgagcggc aaaaatcaa gtcgttaag ttgctaaga ctcatgcag caaaaggca gaagaacaa accaaatcg atttttaca acaaacgaa atccgaaat ttatcccaa gatgtggga attaaccct tccaagttt ttctctgtc ttgcaaagt ttaaacatt caaggtttt attttgaat aaaggcgcc aatcgatat caacttgtc actgccgat ttgtaa
- >TC009331 RA
cctcacttt ttcaaacaa tttgttttt tatggtgaa ataattaaa tatgaataa aaataaccg ttcctggtt taagaagaa accttctca aattaactt aaaccttgt caatgtcgc aaccaactt taacaacga accgaaata tggttatta cggatttaa actgtactt ccctgaatc aacagacga cgaaaccaa agatgagac tctccttaa atacgtgct tcattttga aaatttcat taaacggca tagtgccaa tttttcgct tggcgaaat cgaggccca aaaatgctt tgtgattga ctataagct cataacttc agacgaaga attaagtgc gggatggaa aggtttaga aacggagat aatcacaaa cactgatct ttgcctaag ttgtatggg tttagccat gcaccagtt tatcatgaa ggattacga taacaacag taggaaaaa aagtttagg gataattag agcgagact tatcaatta cgagcccat tcaacaaat aaaagacat acgggtcaa taattatgg tcgtttaat ttcgctcaa aggcactgt gataaaagc cgctaatgt taaaattat gtaccaata tatggcatt ttcatgtgc aacttgtac aggaattca agtcgtcaa acaaccaga taacatttt tacagtacc taacaaatg tttaacgga agggtgcaa agcccgctc taatttcca agccttgca ttcgtcccc tttcacacg aacgattag ctggcaaca catcaaaat tcaagaatt gattggaaa cgatgagtt tgaaaacgg gcgcgttcc cagaacttt ggaatgcga attgactga agatttggt caattcttg tgtcccggg agatgatgt cacgataac aggcgttat aaaagtgcg taataatgc cgaaacttc gtataaaaa taaggagaa aagtgtttt tctccttta tttggacgt tgtttcagt tgtaaataa caaaaatca gaatgaggg tacttatgg ggcttcgga acggataac tttcaatat tactgacta ttacgcaat tcaaaaaat tcatgccga gccttatct ctttcgttt ccttgtcca atcactatg ccccacaat ttacggcca cgagatcgt caaagcagg ccttcttct tgccctttt tggcggcac aaaatcgtc gaaatttcg tgccgaatc gcacgtttt aatggttgg cgaccctgg aatcggtaa atcccagat gttgcatgc ttgtgtaaa cgtagcccc cagaggtgt gtacgtttg tggtaacac aagtactgg atctggatt aactgttac aatgactcg ggaagctaa aggagaata ttccctaga agcaggggc gctaatgtt ggccgatca ggggtgttg ttgtataga cgagttcga taaaatgcc cacacaaca tgcttgtct cttagaagt aatggagca gcaaagtat tagtatcgc aaaggctgg gattgtatg tactttacc aacacgtgc tacgatttt agcggctgc taatccagc tggggggca ttataataa agctaaaac tatagctga aaatttgaa aattagttc gccgatgtt gtcacgatt tgatttggt ttttatact tctggatca accaaatga ggatttgga tatgaggct atctgaaca tattctagc tttgcattc aaggcgaaa tggatcaaa tgtgtcaaa aaattcaac attggccga aggtgtaaa caactcctt gcgaggacg cttgtctct tcaagacgg ggaggaaat agactattt gccgcactc cttatttcg aaaatacat agcttatgc gcaaaaata tgtgaatcc ccaactttc agatgacgc taaacaagt attgaaaga tttttattt ccaactccg aaaggagtt tcaaaatgg ggatagtac ccctgttac gaccagaca actaaattc tttaatgag actgactca ggctagagc aaaagccga actgagaga agaagcaac taaagagga cgctcaaga cgttgtgga aattatgcg acaaacttt aatagacat ttttaccga taatgtcgg aattttaga taccactcg gtcgcaaaa tggggccgg aatgagcgg caaaaatca agtcgttaa gttgctaag actcatgca gcaaaaggc agaagaaca aaccaaatc gatttttac aacaaacga aatccgaaa tttatccca agatgtggg aattaaccc ttccaagtt tttctctgt cttgcaaag tttaaacat tcaaggttt tattttgaa taaaggcgc caatcgata tcaacttgt cactgccga tttgtaatt atgtaattt tgtgaataa aactatctt tttgaa
