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Gene information
General information
TC009331 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 464275..467679
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009331
    iBeetle screen iB_01529

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009331 RA
      MNKNNRSWF KKKPSQINL NLVNVATNF NNEPKYGYY 
      GFKLYFPES TDDETKDET LLKYVLHFE NFIKRHSAN 
      FSLGEIEAQ KCFVIDYKL ITSDEELSA GWKGLETEI 
      ITNTDLCLS CMGLAMHQF IMKDYDNNS RKKSLGIIR 
      ARLINYEPI QQIKDIRVN NYGRLISLK GTVIKAANV 
      KIMYQYMAF SCATCTGIQ VVKQPDNIF TVPNKCLTE 
      GCKARSNFQ ALHSSPFTR TISWQHIKI QELIGNDEF 
      ENGRVPRTL ECELTEDLV NSCVPGDDV TITGVIKVR 
      NNAETSYKN KEKSVFLLY LDVVSVVNN KNQNEGTYG 
      ASERITFNI TDYYAIQKI HAEPYLFRF LVQSLCPTI 
      YGHEIVKAG LLLALFGGT KSSKFRAES HVLMVGDPG 
      IGKSQMLHA CVNVAPRGV YVCGNTSTG SGLTVTMTR 
      EAKGEYSLE AGALMLADQ GCCCIDEFD KMPTQHACL 
      LEVMEQQSI SIAKAGIVC TLPTRATIL AAANPAGGH 
      YNKAKTIAE NLKISSPML SRFDLVFIL LDQPNEDLD 
      MRLSEHILA LHSRRNGSN VSKNSTLAE GVNNSLRGR 
      LSLQDGEEI DYLPHSLFR KYIAYAQKY VNPQLSDDA 
      KQVLKDFYF QLRKEFQNG DSTPVTTRQ LNSLMRLTQ 
      ARAKAELRE EATKEDAQD VVEIMRQTL IDIFTDNVG 
      ILDTTRSQN GAGMSGKNQ VVKLLRLMQ QKAEEQTKS 
      IFTTNEIRN LSQDVGINP SKFFSVLQS LNIQGFILN 
      KGANRYQLV TADL
    CDS sequence
    • >TC009331 RA
      atgaataaa aataaccgt tcctggttt aagaagaaa 
      ccttctcaa attaactta aaccttgtc aatgtcgca 
      accaacttt aacaacgaa ccgaaatat ggttattac 
      ggatttaaa ctgtacttc cctgaatca acagacgac 
      gaaaccaaa gatgagact ctccttaaa tacgtgctt 
      cattttgaa aatttcatt aaacggcat agtgccaat 
      ttttcgctt ggcgaaatc gaggcccaa aaatgcttt 
      gtgattgac tataagctc ataacttca gacgaagaa 
      ttaagtgcg ggatggaaa ggtttagaa acggagata 
      atcacaaac actgatctt tgcctaagt tgtatgggt 
      ttagccatg caccagttt atcatgaag gattacgat 
      aacaacagt aggaaaaaa agtttaggg ataattaga 
      gcgagactt atcaattac gagcccatt caacaaata 
      aaagacata cgggtcaat aattatggt cgtttaatt 
      tcgctcaaa ggcactgtg ataaaagcc gctaatgtt 
      aaaattatg taccaatat atggcattt tcatgtgca 
      acttgtaca ggaattcaa gtcgtcaaa caaccagat 
      aacattttt acagtacct aacaaatgt ttaacggaa 
      gggtgcaaa gcccgctct aatttccaa gccttgcat 
      tcgtcccct ttcacacga acgattagc tggcaacac 
      atcaaaatt caagaattg attggaaac gatgagttt 
      gaaaacggg cgcgttccc agaactttg gaatgcgaa 
      ttgactgaa gatttggtc aattcttgt gtcccggga 
      gatgatgtc acgataaca ggcgttata aaagtgcgt 
      aataatgcc gaaacttcg tataaaaat aaggagaaa 
      agtgttttt ctcctttat ttggacgtt gtttcagtt 
      gtaaataac aaaaatcag aatgagggt acttatggg 
      gcttcggaa cggataact ttcaatatt actgactat 
      tacgcaatt caaaaaatt catgccgag ccttatctc 
      tttcgtttc cttgtccaa tcactatgc cccacaatt 
      tacggccac gagatcgtc aaagcaggc cttcttctt 
      gcccttttt ggcggcaca aaatcgtcg aaatttcgt 
      gccgaatcg cacgtttta atggttggc gaccctgga 
      atcggtaaa tcccagatg ttgcatgct tgtgtaaac 
      gtagccccc agaggtgtg tacgtttgt ggtaacaca 
      agtactgga tctggatta actgttaca atgactcgg 
      gaagctaaa ggagaatat tccctagaa gcaggggcg 
      ctaatgttg gccgatcag gggtgttgt tgtatagac 
      gagttcgat aaaatgccc acacaacat gcttgtctc 
      ttagaagta atggagcag caaagtatt agtatcgca 
      aaggctggg attgtatgt actttacca acacgtgct 
      acgatttta gcggctgct aatccagct ggggggcat 
      tataataaa gctaaaact atagctgaa aatttgaaa 
      attagttcg ccgatgttg tcacgattt gatttggtt 
      tttatactt ctggatcaa ccaaatgag gatttggat 
      atgaggcta tctgaacat attctagct ttgcattca 
      aggcgaaat ggatcaaat gtgtcaaaa aattcaaca 
      ttggccgaa ggtgtaaac aactccttg cgaggacgc 
      ttgtctctt caagacggg gaggaaata gactatttg 
      ccgcactcc ttatttcga aaatacata gcttatgcg 
      caaaaatat gtgaatccc caactttca gatgacgct 
      aaacaagta ttgaaagat ttttatttc caactccga 
      aaggagttt caaaatggg gatagtacc cctgttacg 
      accagacaa ctaaattct ttaatgaga ctgactcag 
      gctagagca aaagccgaa ctgagagaa gaagcaact 
      aaagaggac gctcaagac gttgtggaa attatgcga 
      caaacttta atagacatt tttaccgat aatgtcgga 
      attttagat accactcgg tcgcaaaat ggggccgga 
      atgagcggc aaaaatcaa gtcgttaag ttgctaaga 
      ctcatgcag caaaaggca gaagaacaa accaaatcg 
      atttttaca acaaacgaa atccgaaat ttatcccaa 
      gatgtggga attaaccct tccaagttt ttctctgtc 
      ttgcaaagt ttaaacatt caaggtttt attttgaat 
      aaaggcgcc aatcgatat caacttgtc actgccgat 
      ttgtaa
    mRNA sequence
    • >TC009331 RA
      cctcacttt ttcaaacaa tttgttttt tatggtgaa 
      ataattaaa tatgaataa aaataaccg ttcctggtt 
      taagaagaa accttctca aattaactt aaaccttgt 
      caatgtcgc aaccaactt taacaacga accgaaata 
      tggttatta cggatttaa actgtactt ccctgaatc 
      aacagacga cgaaaccaa agatgagac tctccttaa 
      atacgtgct tcattttga aaatttcat taaacggca 
      tagtgccaa tttttcgct tggcgaaat cgaggccca 
      aaaatgctt tgtgattga ctataagct cataacttc 
      agacgaaga attaagtgc gggatggaa aggtttaga 
      aacggagat aatcacaaa cactgatct ttgcctaag 
      ttgtatggg tttagccat gcaccagtt tatcatgaa 
      ggattacga taacaacag taggaaaaa aagtttagg 
      gataattag agcgagact tatcaatta cgagcccat 
      tcaacaaat aaaagacat acgggtcaa taattatgg 
      tcgtttaat ttcgctcaa aggcactgt gataaaagc 
      cgctaatgt taaaattat gtaccaata tatggcatt 
      ttcatgtgc aacttgtac aggaattca agtcgtcaa 
      acaaccaga taacatttt tacagtacc taacaaatg 
      tttaacgga agggtgcaa agcccgctc taatttcca 
      agccttgca ttcgtcccc tttcacacg aacgattag 
      ctggcaaca catcaaaat tcaagaatt gattggaaa 
      cgatgagtt tgaaaacgg gcgcgttcc cagaacttt 
      ggaatgcga attgactga agatttggt caattcttg 
      tgtcccggg agatgatgt cacgataac aggcgttat 
      aaaagtgcg taataatgc cgaaacttc gtataaaaa 
      taaggagaa aagtgtttt tctccttta tttggacgt 
      tgtttcagt tgtaaataa caaaaatca gaatgaggg 
      tacttatgg ggcttcgga acggataac tttcaatat 
      tactgacta ttacgcaat tcaaaaaat tcatgccga 
      gccttatct ctttcgttt ccttgtcca atcactatg 
      ccccacaat ttacggcca cgagatcgt caaagcagg 
      ccttcttct tgccctttt tggcggcac aaaatcgtc 
      gaaatttcg tgccgaatc gcacgtttt aatggttgg 
      cgaccctgg aatcggtaa atcccagat gttgcatgc 
      ttgtgtaaa cgtagcccc cagaggtgt gtacgtttg 
      tggtaacac aagtactgg atctggatt aactgttac 
      aatgactcg ggaagctaa aggagaata ttccctaga 
      agcaggggc gctaatgtt ggccgatca ggggtgttg 
      ttgtataga cgagttcga taaaatgcc cacacaaca 
      tgcttgtct cttagaagt aatggagca gcaaagtat 
      tagtatcgc aaaggctgg gattgtatg tactttacc 
      aacacgtgc tacgatttt agcggctgc taatccagc 
      tggggggca ttataataa agctaaaac tatagctga 
      aaatttgaa aattagttc gccgatgtt gtcacgatt 
      tgatttggt ttttatact tctggatca accaaatga 
      ggatttgga tatgaggct atctgaaca tattctagc 
      tttgcattc aaggcgaaa tggatcaaa tgtgtcaaa 
      aaattcaac attggccga aggtgtaaa caactcctt 
      gcgaggacg cttgtctct tcaagacgg ggaggaaat 
      agactattt gccgcactc cttatttcg aaaatacat 
      agcttatgc gcaaaaata tgtgaatcc ccaactttc 
      agatgacgc taaacaagt attgaaaga tttttattt 
      ccaactccg aaaggagtt tcaaaatgg ggatagtac 
      ccctgttac gaccagaca actaaattc tttaatgag 
      actgactca ggctagagc aaaagccga actgagaga 
      agaagcaac taaagagga cgctcaaga cgttgtgga 
      aattatgcg acaaacttt aatagacat ttttaccga 
      taatgtcgg aattttaga taccactcg gtcgcaaaa 
      tggggccgg aatgagcgg caaaaatca agtcgttaa 
      gttgctaag actcatgca gcaaaaggc agaagaaca 
      aaccaaatc gatttttac aacaaacga aatccgaaa 
      tttatccca agatgtggg aattaaccc ttccaagtt 
      tttctctgt cttgcaaag tttaaacat tcaaggttt 
      tattttgaa taaaggcgc caatcgata tcaacttgt 
      cactgccga tttgtaatt atgtaattt tgtgaataa 
      aactatctt tttgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY