General information | |||
TC009315 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 276096..281293 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC009315 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01526 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
labium partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax cuticle dorsal structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment number decreased # of remaining segments - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)
adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 4 (16 dpi)
adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (22 dpi)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
Comment: in some cases both oblique muscles are rounded and in other cases only the outer or the inner oblique muscle is rounded |
- >TC009315 RA
MGKRRRLNK FISKWIKSD LPEDRSTVL LLGVTRIPT SPLPARRPC TLPLDPSQT LEISSQKSS SAQSRRTSF STRRRHTRS SSIKRRPGD DSKCSSSSW ENPNDHDDE LTSPSSNCV SGYENAAST TETVMDRSV DSIGTCSLD VEASADLSD WSEASSGGT LRSASIRTP TSEPHLPSY LSLACTVNG YSTTTNYDP VRLARSRDT SPHRLDNDN LTPKNPPTT YSIQNNLLS PPNLVPLPT HIKPKMTDH TITHHHHYQ STKTMTFQK HTESHHFTS KVYTKDTID ACYDHKKLT NKCMPKMNG QSKMNVVET KSFIEQRVE RLYGPGALA QGFFVSKKQ KHESECENS YVEENGIES PMKQSNSSP ALPVLRHLR PEFRAQLPI LSPRKGVEN SMQKSCTVP TLKDEVKVN GHSKQIAAS EGDNVAKMT IKEETGEKD GHHFLKILQ NETNRLLQL ADKVEMELS NTELSEEII GKIHSASGK ARLLVSQKM QQFKGLCTN NITQGENEA FPTTNEDLQ GFWDMVMLQ VDQVDALFK EIELLKANN WQEVKPSQP VNNVTNGKA KKIVNRVKA TSQASEEAR KKREAQRKQ MIEERRKAM KAQKPVESI EIFVPESS
- >TC009315 RA
atgggtaaa cgtcgacgt ttgaataag ttcatcagt aaatggatc aagagtgac ctcccggag gatcgctca acggtgctg ctgctcggc gtcaccagg ataccgacc tctccgtta ccggcccgt cgtccctgc acccttcct ctcgaccca tcccaaacc cttgagatc agctcccaa aaatcgtca agtgcacag tcacgtcgg acgtcgttt tcgacaaga cgacgacat actaggagt tcgtcgatc aaacgacgg cccggcgac gattcaaaa tgctcctca tcctcatgg gagaatccc aacgaccac gacgacgaa ctcacgtcg ccgtcttct aattgcgta tccggttat gaaaacgct gcttccacc accgaaacg gtgatggac cgatctgtc gactctatc gggacttgc tctctagac gttgaagct tcggctgat ctttcagac tggtctgaa gcctcatct ggtggcacc ctccggagc gctagcatc agaaccccc acatctgag ccccattta ccttcatat ctcagtcta gcctgcact gtaaacggc tattccacc acaaccaac tacgaccca gtacgtctt gctcgttcc agagacact tctccccat cgtctcgat aatgacaat ttgacacca aaaaatcca ccaacaact tattccatc cagaacaat cttctttca cccccaaat ctcgtccct ttaccaacc cacatcaag cccaaaatg accgaccat accatcacc catcatcat cactaccaa tcaaccaag accatgacg ttccaaaaa cacaccgaa tctcaccat ttcacctca aaagtctac acaaaggat accatcgac gcttgctac gaccacaag aaactcacc aataaatgc atgccaaaa atgaacgga cagagcaaa atgaatgtt gttgaaaca aaatcgttc attgagcag agagttgag aggttgtac ggacctggg gctctagct cagggattt ttcgtctca aagaagcaa aaacatgaa tcggaatgt gagaattct tatgtggaa gaaaacgga attgaatcg ccaatgaaa cagagtaat agtagtcca gctttgcca gttttgaga catttgaga cctgaattt cgggcacaa ttaccgatt ttgagtccg aggaaaggg gtcgagaat tcaatgcag aaaagttgt actgtacct acgttgaaa gatgaggtt aaagttaat gggcatagt aagcaaatt gcggcaagt gaaggggat aatgttgcc aagatgacc attaaggaa gaaacaggt gaaaaggac ggtcaccac tttttgaaa atcctgcag aacgaaacg aaccgactt ttacaactg gctgacaaa gtagaaatg gaattatcc aacactgaa ctgtctgaa gaaatcatc gggaaaatt cattcggct tcggggaaa gccagactt ttagtatca cagaaaatg caacagttc aaaggacta tgcacgaat aacatcaca cagggtgaa aatgaagct tttcccacc actaatgag gacttgcaa ggcttttgg gacatggtg atgcttcag gtcgaccaa gtggatgct ctcttcaag gaaatcgag ttgctgaag gccaacaat tggcaagag gtgaagcca tcacaacct gtcaataat gtgaccaat ggcaaagca aagaaaata gtgaataga gtaaaagca acgtcacag gcgagcgaa gaagctcga aagaaacgc gaagcccaa cgaaaacag atgattgaa gagaggaga aaggcaatg aaagcccaa aaaccagta gaaagtatt gaaatattc gtgcctgaa tctagctga
- >TC009315 RA
cgttctaaa atcagttta ttcgaatca tgctttgag ctagatgtg atgtaggta cacacaaaa tggatcggc agttttgtg gtttaaatt ttcgtttga aaatgggta aacgtcgac gtttgaata agttcatca gtaaatgga tcaagagtg acctcccgg aggatcgct caacggtgc tgctgctcg gcgtcacca ggataccga cctctccgt taccggccc gtcgtccct gcacccttc ctctcgacc catcccaaa cccttgaga tcagctccc aaaaatcgt caagtgcac agtcacgtc ggacgtcgt tttcgacaa gacgacgac atactagga gttcgtcga tcaaacgac ggcccggcg acgattcaa aatgctcct catcctcat gggagaatc ccaacgacc acgacgacg aactcacgt cgccgtctt ctaattgcg tatccggtt atgaaaacg ctgcttcca ccaccgaaa cggtgatgg accgatctg tcgactcta tcgggactt gctctctag acgttgaag cttcggctg atctttcag actggtctg aagcctcat ctggtggca ccctccgga gcgctagca tcagaaccc ccacatctg agccccatt taccttcat atctcagtc tagcctgca ctgtaaacg gctattcca ccacaacca actacgacc cagtacgtc ttgctcgtt ccagagaca cttctcccc atcgtctcg ataatgaca atttgacac caaaaaatc caccaacaa cttattcca tccagaaca atcttcttt cacccccaa atctcgtcc ctttaccaa cccacatca agcccaaaa tgaccgacc ataccatca cccatcatc atcactacc aatcaacca agaccatga cgttccaaa aacacaccg aatctcacc atttcacct caaaagtct acacaaagg ataccatcg acgcttgct acgaccaca agaaactca ccaataaat gcatgccaa aaatgaacg gacagagca aaatgaatg ttgttgaaa caaaatcgt tcattgagc agagagttg agaggttgt acggacctg gggctctag ctcagggat ttttcgtct caaagaagc aaaaacatg aatcggaat gtgagaatt cttatgtgg aagaaaacg gaattgaat cgccaatga aacagagta atagtagtc cagctttgc cagttttga gacatttga gacctgaat ttcgggcac aattaccga ttttgagtc cgaggaaag gggtcgaga attcaatgc agaaaagtt gtactgtac ctacgttga aagatgagg ttaaagtta atgggcata gtaagcaaa ttgcggcaa gtgaagggg ataatgttg ccaagatga ccattaagg aagaaacag gtgaaaagg acggtcacc actttttga aaatcctgc agaacgaaa cgaaccgac ttttacaac tggctgaca aagtagaaa tggaattat ccaacactg aactgtctg aagaaatca tcgggaaaa ttcattcgg cttcgggga aagccagac ttttagtat cacagaaaa tgcaacagt tcaaaggac tatgcacga ataacatca cacagggtg aaaatgaag cttttccca ccactaatg aggacttgc aaggctttt gggacatgg tgatgcttc aggtcgacc aagtggatg ctctcttca aggaaatcg agttgctga aggccaaca attggcaag aggtgaagc catcacaac ctgtcaata atgtgacca atggcaaag caaagaaaa tagtgaata gagtaaaag caacgtcac aggcgagcg aagaagctc gaaagaaac gcgaagccc aacgaaaac agatgattg aagagagga gaaaggcaa tgaaagccc aaaaaccag tagaaagta ttgaaatat tcgtgcctg aatctagct gaaatttat ccaaatttt agaagtttt atatttatt acctacaga ataatattt tgtgaaatt cctttaaga tctgtggtg tatggtgtt ttttgaaaa ttttgttgt gacctgcac tgaataatt catgttaag actgagttt gaaattttt tatcataat actaaaata ataaccaaa taactaagt actctgctg gtacattcc cagtgcttt tataaaaca ggtgtattt tttcttgta atttttacc atttgcatg ccgatttgt tatgtaatg ttattgtat agttaaatt ttttattac agttgggtt tctttcctc acactaaat attttataa tttgcgaaa tcaaatata agtaagaag taccattta ttatattcg aattgtatt tatacacac aataaattt tcaactttt accaacact tggcgcttg taatcacca cctgtcatt aattcttac aagttcttt taacttaaa tttgaacat tttg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
