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Gene information
General information
TC009315 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 276096..281293
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009315
    iBeetle screen iB_01526

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    labium partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax cuticle dorsal structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased # of remaining segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Pupal morphology
    oblique dorsal thoracic muscles partially rounded - Number of animals showing the phenotype: 6

    Comment: in some cases both oblique muscles are rounded and in other cases only the outer or the inner oblique muscle is rounded
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009315 RA
      MGKRRRLNK FISKWIKSD LPEDRSTVL LLGVTRIPT 
      SPLPARRPC TLPLDPSQT LEISSQKSS SAQSRRTSF 
      STRRRHTRS SSIKRRPGD DSKCSSSSW ENPNDHDDE 
      LTSPSSNCV SGYENAAST TETVMDRSV DSIGTCSLD 
      VEASADLSD WSEASSGGT LRSASIRTP TSEPHLPSY 
      LSLACTVNG YSTTTNYDP VRLARSRDT SPHRLDNDN 
      LTPKNPPTT YSIQNNLLS PPNLVPLPT HIKPKMTDH 
      TITHHHHYQ STKTMTFQK HTESHHFTS KVYTKDTID 
      ACYDHKKLT NKCMPKMNG QSKMNVVET KSFIEQRVE 
      RLYGPGALA QGFFVSKKQ KHESECENS YVEENGIES 
      PMKQSNSSP ALPVLRHLR PEFRAQLPI LSPRKGVEN 
      SMQKSCTVP TLKDEVKVN GHSKQIAAS EGDNVAKMT 
      IKEETGEKD GHHFLKILQ NETNRLLQL ADKVEMELS 
      NTELSEEII GKIHSASGK ARLLVSQKM QQFKGLCTN 
      NITQGENEA FPTTNEDLQ GFWDMVMLQ VDQVDALFK 
      EIELLKANN WQEVKPSQP VNNVTNGKA KKIVNRVKA 
      TSQASEEAR KKREAQRKQ MIEERRKAM KAQKPVESI 
      EIFVPESS
    CDS sequence
    • >TC009315 RA
      atgggtaaa cgtcgacgt ttgaataag ttcatcagt 
      aaatggatc aagagtgac ctcccggag gatcgctca 
      acggtgctg ctgctcggc gtcaccagg ataccgacc 
      tctccgtta ccggcccgt cgtccctgc acccttcct 
      ctcgaccca tcccaaacc cttgagatc agctcccaa 
      aaatcgtca agtgcacag tcacgtcgg acgtcgttt 
      tcgacaaga cgacgacat actaggagt tcgtcgatc 
      aaacgacgg cccggcgac gattcaaaa tgctcctca 
      tcctcatgg gagaatccc aacgaccac gacgacgaa 
      ctcacgtcg ccgtcttct aattgcgta tccggttat 
      gaaaacgct gcttccacc accgaaacg gtgatggac 
      cgatctgtc gactctatc gggacttgc tctctagac 
      gttgaagct tcggctgat ctttcagac tggtctgaa 
      gcctcatct ggtggcacc ctccggagc gctagcatc 
      agaaccccc acatctgag ccccattta ccttcatat 
      ctcagtcta gcctgcact gtaaacggc tattccacc 
      acaaccaac tacgaccca gtacgtctt gctcgttcc 
      agagacact tctccccat cgtctcgat aatgacaat 
      ttgacacca aaaaatcca ccaacaact tattccatc 
      cagaacaat cttctttca cccccaaat ctcgtccct 
      ttaccaacc cacatcaag cccaaaatg accgaccat 
      accatcacc catcatcat cactaccaa tcaaccaag 
      accatgacg ttccaaaaa cacaccgaa tctcaccat 
      ttcacctca aaagtctac acaaaggat accatcgac 
      gcttgctac gaccacaag aaactcacc aataaatgc 
      atgccaaaa atgaacgga cagagcaaa atgaatgtt 
      gttgaaaca aaatcgttc attgagcag agagttgag 
      aggttgtac ggacctggg gctctagct cagggattt 
      ttcgtctca aagaagcaa aaacatgaa tcggaatgt 
      gagaattct tatgtggaa gaaaacgga attgaatcg 
      ccaatgaaa cagagtaat agtagtcca gctttgcca 
      gttttgaga catttgaga cctgaattt cgggcacaa 
      ttaccgatt ttgagtccg aggaaaggg gtcgagaat 
      tcaatgcag aaaagttgt actgtacct acgttgaaa 
      gatgaggtt aaagttaat gggcatagt aagcaaatt 
      gcggcaagt gaaggggat aatgttgcc aagatgacc 
      attaaggaa gaaacaggt gaaaaggac ggtcaccac 
      tttttgaaa atcctgcag aacgaaacg aaccgactt 
      ttacaactg gctgacaaa gtagaaatg gaattatcc 
      aacactgaa ctgtctgaa gaaatcatc gggaaaatt 
      cattcggct tcggggaaa gccagactt ttagtatca 
      cagaaaatg caacagttc aaaggacta tgcacgaat 
      aacatcaca cagggtgaa aatgaagct tttcccacc 
      actaatgag gacttgcaa ggcttttgg gacatggtg 
      atgcttcag gtcgaccaa gtggatgct ctcttcaag 
      gaaatcgag ttgctgaag gccaacaat tggcaagag 
      gtgaagcca tcacaacct gtcaataat gtgaccaat 
      ggcaaagca aagaaaata gtgaataga gtaaaagca 
      acgtcacag gcgagcgaa gaagctcga aagaaacgc 
      gaagcccaa cgaaaacag atgattgaa gagaggaga 
      aaggcaatg aaagcccaa aaaccagta gaaagtatt 
      gaaatattc gtgcctgaa tctagctga
    mRNA sequence
    • >TC009315 RA
      cgttctaaa atcagttta ttcgaatca tgctttgag 
      ctagatgtg atgtaggta cacacaaaa tggatcggc 
      agttttgtg gtttaaatt ttcgtttga aaatgggta 
      aacgtcgac gtttgaata agttcatca gtaaatgga 
      tcaagagtg acctcccgg aggatcgct caacggtgc 
      tgctgctcg gcgtcacca ggataccga cctctccgt 
      taccggccc gtcgtccct gcacccttc ctctcgacc 
      catcccaaa cccttgaga tcagctccc aaaaatcgt 
      caagtgcac agtcacgtc ggacgtcgt tttcgacaa 
      gacgacgac atactagga gttcgtcga tcaaacgac 
      ggcccggcg acgattcaa aatgctcct catcctcat 
      gggagaatc ccaacgacc acgacgacg aactcacgt 
      cgccgtctt ctaattgcg tatccggtt atgaaaacg 
      ctgcttcca ccaccgaaa cggtgatgg accgatctg 
      tcgactcta tcgggactt gctctctag acgttgaag 
      cttcggctg atctttcag actggtctg aagcctcat 
      ctggtggca ccctccgga gcgctagca tcagaaccc 
      ccacatctg agccccatt taccttcat atctcagtc 
      tagcctgca ctgtaaacg gctattcca ccacaacca 
      actacgacc cagtacgtc ttgctcgtt ccagagaca 
      cttctcccc atcgtctcg ataatgaca atttgacac 
      caaaaaatc caccaacaa cttattcca tccagaaca 
      atcttcttt cacccccaa atctcgtcc ctttaccaa 
      cccacatca agcccaaaa tgaccgacc ataccatca 
      cccatcatc atcactacc aatcaacca agaccatga 
      cgttccaaa aacacaccg aatctcacc atttcacct 
      caaaagtct acacaaagg ataccatcg acgcttgct 
      acgaccaca agaaactca ccaataaat gcatgccaa 
      aaatgaacg gacagagca aaatgaatg ttgttgaaa 
      caaaatcgt tcattgagc agagagttg agaggttgt 
      acggacctg gggctctag ctcagggat ttttcgtct 
      caaagaagc aaaaacatg aatcggaat gtgagaatt 
      cttatgtgg aagaaaacg gaattgaat cgccaatga 
      aacagagta atagtagtc cagctttgc cagttttga 
      gacatttga gacctgaat ttcgggcac aattaccga 
      ttttgagtc cgaggaaag gggtcgaga attcaatgc 
      agaaaagtt gtactgtac ctacgttga aagatgagg 
      ttaaagtta atgggcata gtaagcaaa ttgcggcaa 
      gtgaagggg ataatgttg ccaagatga ccattaagg 
      aagaaacag gtgaaaagg acggtcacc actttttga 
      aaatcctgc agaacgaaa cgaaccgac ttttacaac 
      tggctgaca aagtagaaa tggaattat ccaacactg 
      aactgtctg aagaaatca tcgggaaaa ttcattcgg 
      cttcgggga aagccagac ttttagtat cacagaaaa 
      tgcaacagt tcaaaggac tatgcacga ataacatca 
      cacagggtg aaaatgaag cttttccca ccactaatg 
      aggacttgc aaggctttt gggacatgg tgatgcttc 
      aggtcgacc aagtggatg ctctcttca aggaaatcg 
      agttgctga aggccaaca attggcaag aggtgaagc 
      catcacaac ctgtcaata atgtgacca atggcaaag 
      caaagaaaa tagtgaata gagtaaaag caacgtcac 
      aggcgagcg aagaagctc gaaagaaac gcgaagccc 
      aacgaaaac agatgattg aagagagga gaaaggcaa 
      tgaaagccc aaaaaccag tagaaagta ttgaaatat 
      tcgtgcctg aatctagct gaaatttat ccaaatttt 
      agaagtttt atatttatt acctacaga ataatattt 
      tgtgaaatt cctttaaga tctgtggtg tatggtgtt 
      ttttgaaaa ttttgttgt gacctgcac tgaataatt 
      catgttaag actgagttt gaaattttt tatcataat 
      actaaaata ataaccaaa taactaagt actctgctg 
      gtacattcc cagtgcttt tataaaaca ggtgtattt 
      tttcttgta atttttacc atttgcatg ccgatttgt 
      tatgtaatg ttattgtat agttaaatt ttttattac 
      agttgggtt tctttcctc acactaaat attttataa 
      tttgcgaaa tcaaatata agtaagaag taccattta 
      ttatattcg aattgtatt tatacacac aataaattt 
      tcaactttt accaacact tggcgcttg taatcacca 
      cctgtcatt aattcttac aagttcttt taacttaaa 
      tttgaacat tttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY