Status
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Gene information
General information
TC033558
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009287,TC009288
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1865158..1869888
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC033558
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_01521

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC033558 RA
        MVIAKKITI NNRFDGMPK PSDFKTIEE ELPPLKPGE 
        FLAEAVFLS VDPYMRAYA HNIPLGATM VGSQVAKII 
        ESRNDKYPV GKHVVGKFD WRSHTISSG LPTVIQGAG 
        NAPAPYLVP DLGNLPMSY LLGVLGMPG STAYFGFLD 
        ICKPKNGEV VAVSGAAGA VGSIVGQIA KIEGCKVVG 
        ITGSEDKGR FLVDGLGFD GFVNYKTDD VAKKLDQLV 
        PEGIDCYFD NVAGEISST VIGHMRDYG FVGPFRPTT 
        MMWKTRPEN YLYQERSGE AFQQILKWL QAGRMKYHE 
        TITKGFDNM VAAFIEMLQ GKNLGKAIV KVAKSSLQS 
        RLTLYSPIT TNGINKHQM PSSPIKRAI SGGVNMVKA 
        RRYIFEKQF EGLPKSTDL RLVEEELPP IKDGEFLAE 
        AVYLSVDPY MRAYAPRLS LGRTFIGSQ VAKIIESKN 
        DKFPVGKYV VGEFGWRTH SISDGRPLE VGRPGAWLA 
        PELEGLPLS YTLGILGMP GNTAYFGFL ELCKPKSGE 
        TVAVTGAAG AVGSIVGQI AKIKGCTVI GIAGSDEKG 
        KWLVNELKF DHFINYKDP DFEKKLAQA APKGIDCYF 
        DNVGGQIST TIMNKMNLF GRVAVCGAI SGYNDKSAA 
        RADPVQFPL VFSQLKMEG FVVHRWADR WFEGILQNK 
        KWIKEGKLR YKETVTEGF ENMFKAFVD MLQGGNTGK 
        AIVKV
      CDS sequence
      • >TC033558 RA
        atggtaatc gctaaaaaa ataaccatc aacaaccgt 
        ttcgatggc atgcccaaa ccatccgac tttaaaaca 
        attgaagaa gaactaccc ccactcaaa cccggcgaa 
        ttccttgca gaagcggtt tttttaagc gtggacccc 
        tacatgcgg gcctacgcc cataacatc cccctaggg 
        gcgaccatg gtcggctca caagtggcc aaaataatc 
        gaaagtcgc aatgacaaa tacccagtc ggtaagcac 
        gtcgtgggt aaatttgat tggcgcagc cacaccatt 
        tcttcggga cttcccaca gtcattcaa ggggcggga 
        aacgcaccg gcgccatat ctagtcccc gatttgggg 
        aatttaccc atgtcctac cttttgggc gttttggga 
        atgcccggt tccacggca tatttcggt tttttggac 
        atttgcaaa ccgaaaaac ggggaagtt gtggctgtt 
        tcaggcgct gcaggtgct gtggggtcc attgtgggc 
        caaattgcc aaaattgaa gggtgcaaa gttgtgggc 
        atcactggc tcggaggac aaagggcgc tttttggtc 
        gatgggttg ggtttcgat gggtttgtt aattacaaa 
        acggacgat gtcgcgaaa aaattggac cagttggta 
        ccagagggt attgactgt tacttcgat aatgtcgca 
        ggggagatt agttccacg gttattggg catatgagg 
        gattatggg tttgtgggt ccatttcgt cctacaacc 
        atgatgtgg aaaacccgc ccagagaat tacttgtac 
        caagagcgg tcaggtgaa gccttccag caaattctc 
        aagtggttg caagccggg agaatgaaa taccacgaa 
        accatcacc aaaggtttc gataatatg gtggcagcc 
        tttatcgaa atgctgcaa ggcaaaaat ttggggaaa 
        gctatagtt aaagtcgct aaatcatcg ctacaatct 
        cgtttaacc ttgtactct cctatcaca acaaacggt 
        ataaataaa catcaaatg ccgtcatca cccattaaa 
        cgtgcgatt tccgggggc gtaaacatg gtcaaagcc 
        aggcgatac attttcgaa aagcagttc gaaggcctg 
        cccaaatca accgacctg aggctagtt gaagaggag 
        ctgccgcca atcaaggat ggagagttt ttggcggaa 
        gccgtttat ttgagcgta gacccgtac atgagggcc 
        tacgcccct cgcttgtct cttggccgg actttcatt 
        ggatcacaa gtggcaaag ataattgag agcaaaaat 
        gacaaattc ccagtcggg aagtacgtg gttggggaa 
        tttggctgg cgaactcat tccatctct gacggaagg 
        ccgctggaa gtcgggaga cctggggct tggctcgct 
        ccggagctg gagggactg cccttgtcc tacacgctg 
        gggattttg ggaatgccc ggaaacacg gcctatttc 
        ggctttttg gagctgtgc aagcccaaa tccggcgaa 
        acggtggcg gtgacaggc gccgccggt gccgtgggc 
        agcatcgtg ggccaaatc gccaaaatc aagggttgc 
        acagtgatc ggaatcgcc ggttcggac gaaaagggc 
        aaatggctc gtaaacgag ctgaaattc gaccacttc 
        atcaactac aaagacccc gactttgag aaaaaactg 
        gcacaagcg gcccccaaa ggcatcgac tgttacttt 
        gataacgtg ggcggccag atcagcacc accattatg 
        aacaagatg aacctcttc gggagggtg gcagtttgt 
        ggcgccatt tcgggctac aacgacaaa agtgcggcg 
        agggccgac cccgtccag ttccctctg gtgttcagc 
        cagctgaag atggagggg tttgtcgtc caccgatgg 
        gccgatcgg tggtttgaa gggattttg caaaataaa 
        aagtggatc aaagagggg aagttgcgg tacaaggag 
        acggtgact gaagggttc gagaacatg ttcaaggcg 
        ttcgtggat atgctgcaa gggggaaac accgggaaa 
        gccattgtt aaagtttag
      mRNA sequence
      • >TC033558 RA
        actaacata tccaacaaa accaaaatg gtaatcgct 
        aaaaaaata accatcaac aaccgtttc gatggcatg 
        cccaaacca tccgacttt aaaacaatt gaagaagaa 
        ctaccccca ctcaaaccc ggcgaattc cttgcagaa 
        gcggttttt ttaagcgtg gacccctac atgcgggcc 
        tacgcccat aacatcccc ctaggggcg accatggtc 
        ggctcacaa gtggccaaa ataatcgaa agtcgcaat 
        gacaaatac ccagtcggt aagcacgtc gtgggtaaa 
        tttgattgg cgcagccac accatttct tcgggactt 
        cccacagtc attcaaggg gcgggaaac gcaccggcg 
        ccatatcta gtccccgat ttggggaat ttacccatg 
        tcctacctt ttgggcgtt ttgggaatg cccggttcc 
        acggcatat ttcggtttt ttggacatt tgcaaaccg 
        aaaaacggg gaagttgtg gctgtttca ggcgctgca 
        ggtgctgtg gggtccatt gtgggccaa attgccaaa 
        attgaaggg tgcaaagtt gtgggcatc actggctcg 
        gaggacaaa gggcgcttt ttggtcgat gggttgggt 
        ttcgatggg tttgttaat tacaaaacg gacgatgtc 
        gcgaaaaaa ttggaccag ttggtacca gagggtatt 
        gactgttac ttcgataat gtcgcaggg gagattagt 
        tccacggtt attgggcat atgagggat tatgggttt 
        gtgggtcca tttcgtcct acaaccatg atgtggaaa 
        acccgccca gagaattac ttgtaccaa gagcggtca 
        ggtgaagcc ttccagcaa attctcaag tggttgcaa 
        gccgggaga atgaaatac cacgaaacc atcaccaaa 
        ggtttcgat aatatggtg gcagccttt atcgaaatg 
        ctgcaaggc aaaaatttg gggaaagct atagttaaa 
        gtcgctaaa tcatcgcta caatctcgt ttaaccttg 
        tactctcct atcacaaca aacggtata aataaacat 
        caaatgccg tcatcaccc attaaacgt gcgatttcc 
        gggggcgta aacatggtc aaagccagg cgatacatt 
        ttcgaaaag cagttcgaa ggcctgccc aaatcaacc 
        gacctgagg ctagttgaa gaggagctg ccgccaatc 
        aaggatgga gagtttttg gcggaagcc gtttatttg 
        agcgtagac ccgtacatg agggcctac gcccctcgc 
        ttgtctctt ggccggact ttcattgga tcacaagtg 
        gcaaagata attgagagc aaaaatgac aaattccca 
        gtcgggaag tacgtggtt ggggaattt ggctggcga 
        actcattcc atctctgac ggaaggccg ctggaagtc 
        gggagacct ggggcttgg ctcgctccg gagctggag 
        ggactgccc ttgtcctac acgctgggg attttggga 
        atgcccgga aacacggcc tatttcggc tttttggag 
        ctgtgcaag cccaaatcc ggcgaaacg gtggcggtg 
        acaggcgcc gccggtgcc gtgggcagc atcgtgggc 
        caaatcgcc aaaatcaag ggttgcaca gtgatcgga 
        atcgccggt tcggacgaa aagggcaaa tggctcgta 
        aacgagctg aaattcgac cacttcatc aactacaaa 
        gaccccgac tttgagaaa aaactggca caagcggcc 
        cccaaaggc atcgactgt tactttgat aacgtgggc 
        ggccagatc agcaccacc attatgaac aagatgaac 
        ctcttcggg agggtggca gtttgtggc gccatttcg 
        ggctacaac gacaaaagt gcggcgagg gccgacccc 
        gtccagttc cctctggtg ttcagccag ctgaagatg 
        gaggggttt gtcgtccac cgatgggcc gatcggtgg 
        tttgaaggg attttgcaa aataaaaag tggatcaaa 
        gaggggaag ttgcggtac aaggagacg gtgactgaa 
        gggttcgag aacatgttc aaggcgttc gtggatatg 
        ctgcaaggg ggaaacacc gggaaagcc attgttaaa 
        gtttagtct cttattata atttttttg taaataaaa 
        cactgttaa aacg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY