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Gene information
General information
TC033558
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009287,TC009288 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1865158..1869888
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC033558
      iBeetle screen iB_01521

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC033558 RA
        MVIAKKITI NNRFDGMPK PSDFKTIEE ELPPLKPGE 
FLAEAVFLS VDPYMRAYA HNIPLGATM VGSQVAKII 
ESRNDKYPV GKHVVGKFD WRSHTISSG LPTVIQGAG 
NAPAPYLVP DLGNLPMSY LLGVLGMPG STAYFGFLD 
ICKPKNGEV VAVSGAAGA VGSIVGQIA KIEGCKVVG 
ITGSEDKGR FLVDGLGFD GFVNYKTDD VAKKLDQLV 
PEGIDCYFD NVAGEISST VIGHMRDYG FVGPFRPTT 
MMWKTRPEN YLYQERSGE AFQQILKWL QAGRMKYHE 
TITKGFDNM VAAFIEMLQ GKNLGKAIV KVAKSSLQS 
RLTLYSPIT TNGINKHQM PSSPIKRAI SGGVNMVKA 
RRYIFEKQF EGLPKSTDL RLVEEELPP IKDGEFLAE 
AVYLSVDPY MRAYAPRLS LGRTFIGSQ VAKIIESKN 
DKFPVGKYV VGEFGWRTH SISDGRPLE VGRPGAWLA 
PELEGLPLS YTLGILGMP GNTAYFGFL ELCKPKSGE 
TVAVTGAAG AVGSIVGQI AKIKGCTVI GIAGSDEKG 
KWLVNELKF DHFINYKDP DFEKKLAQA APKGIDCYF 
DNVGGQIST TIMNKMNLF GRVAVCGAI SGYNDKSAA 
RADPVQFPL VFSQLKMEG FVVHRWADR WFEGILQNK 
KWIKEGKLR YKETVTEGF ENMFKAFVD MLQGGNTGK 
AIVKV
      CDS sequence
      • >TC033558 RA
        atggtaatc gctaaaaaa ataaccatc aacaaccgt 
ttcgatggc atgcccaaa ccatccgac tttaaaaca 
attgaagaa gaactaccc ccactcaaa cccggcgaa 
ttccttgca gaagcggtt tttttaagc gtggacccc 
tacatgcgg gcctacgcc cataacatc cccctaggg 
gcgaccatg gtcggctca caagtggcc aaaataatc 
gaaagtcgc aatgacaaa tacccagtc ggtaagcac 
gtcgtgggt aaatttgat tggcgcagc cacaccatt 
tcttcggga cttcccaca gtcattcaa ggggcggga 
aacgcaccg gcgccatat ctagtcccc gatttgggg 
aatttaccc atgtcctac cttttgggc gttttggga 
atgcccggt tccacggca tatttcggt tttttggac 
atttgcaaa ccgaaaaac ggggaagtt gtggctgtt 
tcaggcgct gcaggtgct gtggggtcc attgtgggc 
caaattgcc aaaattgaa gggtgcaaa gttgtgggc 
atcactggc tcggaggac aaagggcgc tttttggtc 
gatgggttg ggtttcgat gggtttgtt aattacaaa 
acggacgat gtcgcgaaa aaattggac cagttggta 
ccagagggt attgactgt tacttcgat aatgtcgca 
ggggagatt agttccacg gttattggg catatgagg 
gattatggg tttgtgggt ccatttcgt cctacaacc 
atgatgtgg aaaacccgc ccagagaat tacttgtac 
caagagcgg tcaggtgaa gccttccag caaattctc 
aagtggttg caagccggg agaatgaaa taccacgaa 
accatcacc aaaggtttc gataatatg gtggcagcc 
tttatcgaa atgctgcaa ggcaaaaat ttggggaaa 
gctatagtt aaagtcgct aaatcatcg ctacaatct 
cgtttaacc ttgtactct cctatcaca acaaacggt 
ataaataaa catcaaatg ccgtcatca cccattaaa 
cgtgcgatt tccgggggc gtaaacatg gtcaaagcc 
aggcgatac attttcgaa aagcagttc gaaggcctg 
cccaaatca accgacctg aggctagtt gaagaggag 
ctgccgcca atcaaggat ggagagttt ttggcggaa 
gccgtttat ttgagcgta gacccgtac atgagggcc 
tacgcccct cgcttgtct cttggccgg actttcatt 
ggatcacaa gtggcaaag ataattgag agcaaaaat 
gacaaattc ccagtcggg aagtacgtg gttggggaa 
tttggctgg cgaactcat tccatctct gacggaagg 
ccgctggaa gtcgggaga cctggggct tggctcgct 
ccggagctg gagggactg cccttgtcc tacacgctg 
gggattttg ggaatgccc ggaaacacg gcctatttc 
ggctttttg gagctgtgc aagcccaaa tccggcgaa 
acggtggcg gtgacaggc gccgccggt gccgtgggc 
agcatcgtg ggccaaatc gccaaaatc aagggttgc 
acagtgatc ggaatcgcc ggttcggac gaaaagggc 
aaatggctc gtaaacgag ctgaaattc gaccacttc 
atcaactac aaagacccc gactttgag aaaaaactg 
gcacaagcg gcccccaaa ggcatcgac tgttacttt 
gataacgtg ggcggccag atcagcacc accattatg 
aacaagatg aacctcttc gggagggtg gcagtttgt 
ggcgccatt tcgggctac aacgacaaa agtgcggcg 
agggccgac cccgtccag ttccctctg gtgttcagc 
cagctgaag atggagggg tttgtcgtc caccgatgg 
gccgatcgg tggtttgaa gggattttg caaaataaa 
aagtggatc aaagagggg aagttgcgg tacaaggag 
acggtgact gaagggttc gagaacatg ttcaaggcg 
ttcgtggat atgctgcaa gggggaaac accgggaaa 
gccattgtt aaagtttag
      mRNA sequence
      • >TC033558 RA
        actaacata tccaacaaa accaaaatg gtaatcgct 
aaaaaaata accatcaac aaccgtttc gatggcatg 
cccaaacca tccgacttt aaaacaatt gaagaagaa 
ctaccccca ctcaaaccc ggcgaattc cttgcagaa 
gcggttttt ttaagcgtg gacccctac atgcgggcc 
tacgcccat aacatcccc ctaggggcg accatggtc 
ggctcacaa gtggccaaa ataatcgaa agtcgcaat 
gacaaatac ccagtcggt aagcacgtc gtgggtaaa 
tttgattgg cgcagccac accatttct tcgggactt 
cccacagtc attcaaggg gcgggaaac gcaccggcg 
ccatatcta gtccccgat ttggggaat ttacccatg 
tcctacctt ttgggcgtt ttgggaatg cccggttcc 
acggcatat ttcggtttt ttggacatt tgcaaaccg 
aaaaacggg gaagttgtg gctgtttca ggcgctgca 
ggtgctgtg gggtccatt gtgggccaa attgccaaa 
attgaaggg tgcaaagtt gtgggcatc actggctcg 
gaggacaaa gggcgcttt ttggtcgat gggttgggt 
ttcgatggg tttgttaat tacaaaacg gacgatgtc 
gcgaaaaaa ttggaccag ttggtacca gagggtatt 
gactgttac ttcgataat gtcgcaggg gagattagt 
tccacggtt attgggcat atgagggat tatgggttt 
gtgggtcca tttcgtcct acaaccatg atgtggaaa 
acccgccca gagaattac ttgtaccaa gagcggtca 
ggtgaagcc ttccagcaa attctcaag tggttgcaa 
gccgggaga atgaaatac cacgaaacc atcaccaaa 
ggtttcgat aatatggtg gcagccttt atcgaaatg 
ctgcaaggc aaaaatttg gggaaagct atagttaaa 
gtcgctaaa tcatcgcta caatctcgt ttaaccttg 
tactctcct atcacaaca aacggtata aataaacat 
caaatgccg tcatcaccc attaaacgt gcgatttcc 
gggggcgta aacatggtc aaagccagg cgatacatt 
ttcgaaaag cagttcgaa ggcctgccc aaatcaacc 
gacctgagg ctagttgaa gaggagctg ccgccaatc 
aaggatgga gagtttttg gcggaagcc gtttatttg 
agcgtagac ccgtacatg agggcctac gcccctcgc 
ttgtctctt ggccggact ttcattgga tcacaagtg 
gcaaagata attgagagc aaaaatgac aaattccca 
gtcgggaag tacgtggtt ggggaattt ggctggcga 
actcattcc atctctgac ggaaggccg ctggaagtc 
gggagacct ggggcttgg ctcgctccg gagctggag 
ggactgccc ttgtcctac acgctgggg attttggga 
atgcccgga aacacggcc tatttcggc tttttggag 
ctgtgcaag cccaaatcc ggcgaaacg gtggcggtg 
acaggcgcc gccggtgcc gtgggcagc atcgtgggc 
caaatcgcc aaaatcaag ggttgcaca gtgatcgga 
atcgccggt tcggacgaa aagggcaaa tggctcgta 
aacgagctg aaattcgac cacttcatc aactacaaa 
gaccccgac tttgagaaa aaactggca caagcggcc 
cccaaaggc atcgactgt tactttgat aacgtgggc 
ggccagatc agcaccacc attatgaac aagatgaac 
ctcttcggg agggtggca gtttgtggc gccatttcg 
ggctacaac gacaaaagt gcggcgagg gccgacccc 
gtccagttc cctctggtg ttcagccag ctgaagatg 
gaggggttt gtcgtccac cgatgggcc gatcggtgg 
tttgaaggg attttgcaa aataaaaag tggatcaaa 
gaggggaag ttgcggtac aaggagacg gtgactgaa 
gggttcgag aacatgttc aaggcgttc gtggatatg 
ctgcaaggg ggaaacacc gggaaagcc attgttaaa 
gtttagtct cttattata atttttttg taaataaaa 
cactgttaa aacg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY