| General information | |||
| TC033558
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 1865158..1869888 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033558 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01521 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
- >TC033558 RA
MVIAKKITI NNRFDGMPK PSDFKTIEE ELPPLKPGE FLAEAVFLS VDPYMRAYA HNIPLGATM VGSQVAKII ESRNDKYPV GKHVVGKFD WRSHTISSG LPTVIQGAG NAPAPYLVP DLGNLPMSY LLGVLGMPG STAYFGFLD ICKPKNGEV VAVSGAAGA VGSIVGQIA KIEGCKVVG ITGSEDKGR FLVDGLGFD GFVNYKTDD VAKKLDQLV PEGIDCYFD NVAGEISST VIGHMRDYG FVGPFRPTT MMWKTRPEN YLYQERSGE AFQQILKWL QAGRMKYHE TITKGFDNM VAAFIEMLQ GKNLGKAIV KVAKSSLQS RLTLYSPIT TNGINKHQM PSSPIKRAI SGGVNMVKA RRYIFEKQF EGLPKSTDL RLVEEELPP IKDGEFLAE AVYLSVDPY MRAYAPRLS LGRTFIGSQ VAKIIESKN DKFPVGKYV VGEFGWRTH SISDGRPLE VGRPGAWLA PELEGLPLS YTLGILGMP GNTAYFGFL ELCKPKSGE TVAVTGAAG AVGSIVGQI AKIKGCTVI GIAGSDEKG KWLVNELKF DHFINYKDP DFEKKLAQA APKGIDCYF DNVGGQIST TIMNKMNLF GRVAVCGAI SGYNDKSAA RADPVQFPL VFSQLKMEG FVVHRWADR WFEGILQNK KWIKEGKLR YKETVTEGF ENMFKAFVD MLQGGNTGK AIVKV
- >TC033558 RA
atggtaatc gctaaaaaa ataaccatc aacaaccgt ttcgatggc atgcccaaa ccatccgac tttaaaaca attgaagaa gaactaccc ccactcaaa cccggcgaa ttccttgca gaagcggtt tttttaagc gtggacccc tacatgcgg gcctacgcc cataacatc cccctaggg gcgaccatg gtcggctca caagtggcc aaaataatc gaaagtcgc aatgacaaa tacccagtc ggtaagcac gtcgtgggt aaatttgat tggcgcagc cacaccatt tcttcggga cttcccaca gtcattcaa ggggcggga aacgcaccg gcgccatat ctagtcccc gatttgggg aatttaccc atgtcctac cttttgggc gttttggga atgcccggt tccacggca tatttcggt tttttggac atttgcaaa ccgaaaaac ggggaagtt gtggctgtt tcaggcgct gcaggtgct gtggggtcc attgtgggc caaattgcc aaaattgaa gggtgcaaa gttgtgggc atcactggc tcggaggac aaagggcgc tttttggtc gatgggttg ggtttcgat gggtttgtt aattacaaa acggacgat gtcgcgaaa aaattggac cagttggta ccagagggt attgactgt tacttcgat aatgtcgca ggggagatt agttccacg gttattggg catatgagg gattatggg tttgtgggt ccatttcgt cctacaacc atgatgtgg aaaacccgc ccagagaat tacttgtac caagagcgg tcaggtgaa gccttccag caaattctc aagtggttg caagccggg agaatgaaa taccacgaa accatcacc aaaggtttc gataatatg gtggcagcc tttatcgaa atgctgcaa ggcaaaaat ttggggaaa gctatagtt aaagtcgct aaatcatcg ctacaatct cgtttaacc ttgtactct cctatcaca acaaacggt ataaataaa catcaaatg ccgtcatca cccattaaa cgtgcgatt tccgggggc gtaaacatg gtcaaagcc aggcgatac attttcgaa aagcagttc gaaggcctg cccaaatca accgacctg aggctagtt gaagaggag ctgccgcca atcaaggat ggagagttt ttggcggaa gccgtttat ttgagcgta gacccgtac atgagggcc tacgcccct cgcttgtct cttggccgg actttcatt ggatcacaa gtggcaaag ataattgag agcaaaaat gacaaattc ccagtcggg aagtacgtg gttggggaa tttggctgg cgaactcat tccatctct gacggaagg ccgctggaa gtcgggaga cctggggct tggctcgct ccggagctg gagggactg cccttgtcc tacacgctg gggattttg ggaatgccc ggaaacacg gcctatttc ggctttttg gagctgtgc aagcccaaa tccggcgaa acggtggcg gtgacaggc gccgccggt gccgtgggc agcatcgtg ggccaaatc gccaaaatc aagggttgc acagtgatc ggaatcgcc ggttcggac gaaaagggc aaatggctc gtaaacgag ctgaaattc gaccacttc atcaactac aaagacccc gactttgag aaaaaactg gcacaagcg gcccccaaa ggcatcgac tgttacttt gataacgtg ggcggccag atcagcacc accattatg aacaagatg aacctcttc gggagggtg gcagtttgt ggcgccatt tcgggctac aacgacaaa agtgcggcg agggccgac cccgtccag ttccctctg gtgttcagc cagctgaag atggagggg tttgtcgtc caccgatgg gccgatcgg tggtttgaa gggattttg caaaataaa aagtggatc aaagagggg aagttgcgg tacaaggag acggtgact gaagggttc gagaacatg ttcaaggcg ttcgtggat atgctgcaa gggggaaac accgggaaa gccattgtt aaagtttag
- >TC033558 RA
actaacata tccaacaaa accaaaatg gtaatcgct aaaaaaata accatcaac aaccgtttc gatggcatg cccaaacca tccgacttt aaaacaatt gaagaagaa ctaccccca ctcaaaccc ggcgaattc cttgcagaa gcggttttt ttaagcgtg gacccctac atgcgggcc tacgcccat aacatcccc ctaggggcg accatggtc ggctcacaa gtggccaaa ataatcgaa agtcgcaat gacaaatac ccagtcggt aagcacgtc gtgggtaaa tttgattgg cgcagccac accatttct tcgggactt cccacagtc attcaaggg gcgggaaac gcaccggcg ccatatcta gtccccgat ttggggaat ttacccatg tcctacctt ttgggcgtt ttgggaatg cccggttcc acggcatat ttcggtttt ttggacatt tgcaaaccg aaaaacggg gaagttgtg gctgtttca ggcgctgca ggtgctgtg gggtccatt gtgggccaa attgccaaa attgaaggg tgcaaagtt gtgggcatc actggctcg gaggacaaa gggcgcttt ttggtcgat gggttgggt ttcgatggg tttgttaat tacaaaacg gacgatgtc gcgaaaaaa ttggaccag ttggtacca gagggtatt gactgttac ttcgataat gtcgcaggg gagattagt tccacggtt attgggcat atgagggat tatgggttt gtgggtcca tttcgtcct acaaccatg atgtggaaa acccgccca gagaattac ttgtaccaa gagcggtca ggtgaagcc ttccagcaa attctcaag tggttgcaa gccgggaga atgaaatac cacgaaacc atcaccaaa ggtttcgat aatatggtg gcagccttt atcgaaatg ctgcaaggc aaaaatttg gggaaagct atagttaaa gtcgctaaa tcatcgcta caatctcgt ttaaccttg tactctcct atcacaaca aacggtata aataaacat caaatgccg tcatcaccc attaaacgt gcgatttcc gggggcgta aacatggtc aaagccagg cgatacatt ttcgaaaag cagttcgaa ggcctgccc aaatcaacc gacctgagg ctagttgaa gaggagctg ccgccaatc aaggatgga gagtttttg gcggaagcc gtttatttg agcgtagac ccgtacatg agggcctac gcccctcgc ttgtctctt ggccggact ttcattgga tcacaagtg gcaaagata attgagagc aaaaatgac aaattccca gtcgggaag tacgtggtt ggggaattt ggctggcga actcattcc atctctgac ggaaggccg ctggaagtc gggagacct ggggcttgg ctcgctccg gagctggag ggactgccc ttgtcctac acgctgggg attttggga atgcccgga aacacggcc tatttcggc tttttggag ctgtgcaag cccaaatcc ggcgaaacg gtggcggtg acaggcgcc gccggtgcc gtgggcagc atcgtgggc caaatcgcc aaaatcaag ggttgcaca gtgatcgga atcgccggt tcggacgaa aagggcaaa tggctcgta aacgagctg aaattcgac cacttcatc aactacaaa gaccccgac tttgagaaa aaactggca caagcggcc cccaaaggc atcgactgt tactttgat aacgtgggc ggccagatc agcaccacc attatgaac aagatgaac ctcttcggg agggtggca gtttgtggc gccatttcg ggctacaac gacaaaagt gcggcgagg gccgacccc gtccagttc cctctggtg ttcagccag ctgaagatg gaggggttt gtcgtccac cgatgggcc gatcggtgg tttgaaggg attttgcaa aataaaaag tggatcaaa gaggggaag ttgcggtac aaggagacg gtgactgaa gggttcgag aacatgttc aaggcgttc gtggatatg ctgcaaggg ggaaacacc gggaaagcc attgttaaa gtttagtct cttattata atttttttg taaataaaa cactgttaa aacg
