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Gene information
General information
TC009268 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 76344..102155
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009268
    iBeetle screen iB_01518
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    vitellarium shortened - Number of animals showing the phenotype: 4
    mature egg not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009268 RA
      MIGPHHVCV LYVATAGLQ GSLMGSDEQ EVVLLIYVI 
      VDVVQNKIV GVQQYPIRP QTADINENI LSEQLTSES 
      VLTDELIKS SGRQLEDAL NEFDAYCNS LHIDPHSAG 
      FRLLTDGQL PLRQCLYPE ACRKEIDLP RYYSTFHDL 
      RKEVARFTR KTEEVPQSV ADMLEYLQI KPEVENEFY 
      KREAKDMVN IVQRLISEG YRFEALETI NLTLEPGIC 
      SKDDEIDSN CVVRARGLP WQSSDQDIA KFFSGLNVA 
      KGGVALCLS AQGRRNGEA LIKFESQEH RDMALKRHK 
      HHIGPRYIE VYRASGEDF LSVAGGKSS EAQAFLSRG 
      AAVIVRMRG LPYDCTAKQ VLDFFASGE NSCTVLDGT 
      EGVLFVRKP DGRATGDAF VLFASESDA PKALSKHRE 
      CIGSRYIEL FRSTTAEVQ QVLNRSLEA RTYEPEPLP 
      PLLPHMPLL PQHYITSGT KKECIRLRG LPYEAQVEH 
      ILEFLGEYA KNIIFQGVH MVYNAQGQP SGEAFIQMD 
      SEQSACITA QQKHHRYMT FGKKQRYIE VFQCSGEDM 
      NLVLTGGIP APVSPAKAT PALLSPGML PSIAPLPPT 
      NIPPPPPPN TSVNQISPN ITNWDNQAL FAQQQAQII 
      AQQNLIARQ NQAQAQNEM LLMNQIAQH NLAVLNQNV 
      GPVSPNSPN SVLKNTPMM PHLIAQPPF VMLPPRMAP 
      TLGLPRPQL AYPQHLLPG GAHGVFPVM PHVAVKRSY 
      GDAFNEQAA PAKRAFHAQ GALPVYQHF YPNM
    • >TC009268 RB
      MIGPHHVCV LYVATAGLQ GSLMGSDEQ EVVLLIYVI 
      VDVVQNKIV GVQQYPIRP QTADINENI LSEQLTSES 
      VLTDELIKS SGRQLEDAL NEFDAYCNS LHIDPHSAG 
      FRLLTDGQL PLRQCLYPE ACRKEIDLP RYYSTFHDL 
      RKEVARFTR KTEEVPQSV ADMLEFRTR RTLQFCEYL 
      RLTYKPDLQ IKPEVENEF YKREAKDMV NIVQRLISE 
      GYRFEALET INLTLEPGI CSKDDEIDS NCVVRARGL 
      PWQSSDQDI AKFFSGLNV AKGGVALCL SAQGRRNGE 
      ALIKFESQE HRDMALKRH KHHIGPRYI EVYRASGED 
      FLSVAGGKS SEAQAFLSR GAAVIVRMR GLPYDCTAK 
      QVLDFFASG ENSCTVLDG TEGVLFVRK PDGRATGDA 
      FVLFASESD APKALSKHR ECIGSRYIE LFRSTTAEV 
      QQVLNRSLE ARTYEPEPL PPLLPHMPL LPQHYITSG 
      TKKECIRLR GLPYEAQVE HILEFLGEY AKNIIFQGV 
      HMVYNAQGQ PSGEAFIQM DSEQSACIT AQQKHHRYM 
      TFGKKQRYI EVFQCSGED MNLVLTGGI PAPVSPAKA 
      TPALLSPGM LPSIAPLPP TNIPPPPPP NTSVNQISP 
      NITNWDNQA LFAQQQAQI IAQQNLIAR QNQAQAQNE 
      MLLMNQIAQ HNLAVLNQN VGPVSPNSP NSVLKNTPM 
      MPHLIAQPP FVMLPPRMA PTLGLPRPQ LAYPQHLLP 
      GGAHGVFPV MPHVAVKRS YGDAFNEQA APAKRAFHA 
      QGALPVYQH FYPNM
    CDS sequence
    • >TC009268 RA
      atgatcggg ccccaccat gtttgcgtc ctgtacgtg 
      gccaccgcg gggctccag ggctccctc atgggctcc 
      gacgagcag gaggtggtg ctcctcatc tatgtcatc 
      gtcgacgtc gtccagaat aagattgtg ggcgtgcag 
      cagtaccca atcaggccg caaaccgcc gacatcaac 
      gaaaatatc ctatcggaa cagctcaca tctgagtcg 
      gttttgacc gacgaactc ataaaatca tctggaagg 
      caattggag gacgccctc aacgaattc gatgcctac 
      tgcaatagt ttacacata gacccgcac agtgccggc 
      ttcaggctc ctaacagac ggccaatta ccgttaagg 
      cagtgtctg tatccggaa gcatgtagg aaggaaatt 
      gatctgccg cgttattat agcacgttc catgacctc 
      aggaaagaa gttgccagg tttacgaga aagacggaa 
      gaagtaccc caaagtgtt gccgatatg ttagaatat 
      ttacaaatt aaacccgaa gttgagaat gaattctac 
      aagagggag gctaaggat atggtcaat attgtacaa 
      aggctgatt tcggaagga tacaggttc gaggcgctc 
      gaaacgata aacctgacc ctggagccc ggaatatgt 
      tccaaagac gatgagatt gacagcaac tgcgtggtg 
      agggcgaga ggacttccc tggcagagc agcgatcag 
      gacattgcc aagtttttc agcggactc aatgtcgcc 
      aagggcggc gtagctctg tgcttgtcg gcacaagga 
      cgacggaac ggcgaagct ttaataaaa ttcgagagt 
      caagagcac cgcgacatg gctttgaag aggcacaag 
      caccacatc ggcccccga tacatcgaa gtgtatcgc 
      gcttccggc gaggacttc ctctcggtg gctgggggc 
      aagagttcc gaagcccag gccttcctg tcccggggg 
      gctgccgtc atcgtgcgc atgcggggg ctgccctac 
      gactgcacc gccaaacaa gtcttggat tttttcgcg 
      agtggcgag aactcgtgc accgttttg gacggcact 
      gaaggcgtc ctgttcgtg cggaagccc gacggccga 
      gccactggc gacgcgttc gttctgttt gcgagcgaa 
      tcggacgcg cctaaagct ctctcgaaa catagggaa 
      tgtataggt tctaggtat atcgaattg ttcaggtcg 
      acgacggcc gaggtgcaa caggtgctt aacaggtcg 
      ctggaagcg cggacttac gagccggaa ccgttgccc 
      ccgttatta ccgcacatg cctttattg cctcaacat 
      tatattacg tctgggacc aaaaaggaa tgcattagg 
      ttaagagga ctgccttac gaagctcaa gtcgaacac 
      atcctagag tttctcgga gaatatgcc aaaaatatt 
      atattccaa ggggtgcat atggtctac aatgcacag 
      ggccagccc agcggcgag gccttcatc cagatggac 
      tccgagcag tccgcctgc ataaccgcc cagcagaag 
      caccaccgc tacatgaca ttcggcaaa aagcagcgc 
      tacatcgaa gtattccaa tgctccggc gaggacatg 
      aatctcgta ctgaccggc ggcattccc gcccccgtc 
      tcgcccgca aaggccacc cccgcgcta ctcagtccc 
      ggtatgtta ccttcaatt gcgcctctt ccacctact 
      aatatcccc ccgccgccc ccgccaaac acctcggtc 
      aaccaaata tccccaaac atcacaaac tgggacaac 
      caggccctt ttcgcccaa cagcaggcc cagatcata 
      gcccagcaa aacctgatc gcgcgccaa aaccaagcg 
      caagcccag aacgaaatg ctcctaatg aaccagatt 
      gcgcaacac aatctggcc gtactcaac caaaatgtg 
      ggcccagtg tccccaaac agccccaat tcggtgctg 
      aaaaacacg ccgatgatg ccgcatttg atcgcgcag 
      cccccgttt gtcatgcta cccccgagg atggccccc 
      actttgggg ctccccagg ccccagttg gcctacccc 
      cagcatttg ctgcccggg ggcgcgcat ggggttttt 
      ccggtcatg ccccacgtt gcggttaaa cgcagctat 
      ggggacgcg ttcaatgag caagccgcc ccagccaaa 
      agggccttc catgcacaa ggggccttg cccgtctac 
      caacacttc taccctaac atgtag
    • >TC009268 RB
      atgatcggg ccccaccat gtttgcgtc ctgtacgtg 
      gccaccgcg gggctccag ggctccctc atgggctcc 
      gacgagcag gaggtggtg ctcctcatc tatgtcatc 
      gtcgacgtc gtccagaat aagattgtg ggcgtgcag 
      cagtaccca atcaggccg caaaccgcc gacatcaac 
      gaaaatatc ctatcggaa cagctcaca tctgagtcg 
      gttttgacc gacgaactc ataaaatca tctggaagg 
      caattggag gacgccctc aacgaattc gatgcctac 
      tgcaatagt ttacacata gacccgcac agtgccggc 
      ttcaggctc ctaacagac ggccaatta ccgttaagg 
      cagtgtctg tatccggaa gcatgtagg aaggaaatt 
      gatctgccg cgttattat agcacgttc catgacctc 
      aggaaagaa gttgccagg tttacgaga aagacggaa 
      gaagtaccc caaagtgtt gccgatatg ttagaattc 
      cgcactcgt cgtacgctt cagttttgc gaatattta 
      cggctaacc tacaaacca gatttacaa attaaaccc 
      gaagttgag aatgaattc tacaagagg gaggctaag 
      gatatggtc aatattgta caaaggctg atttcggaa 
      ggatacagg ttcgaggcg ctcgaaacg ataaacctg 
      accctggag cccggaata tgttccaaa gacgatgag 
      attgacagc aactgcgtg gtgagggcg agaggactt 
      ccctggcag agcagcgat caggacatt gccaagttt 
      ttcagcgga ctcaatgtc gccaagggc ggcgtagct 
      ctgtgcttg tcggcacaa ggacgacgg aacggcgaa 
      gctttaata aaattcgag agtcaagag caccgcgac 
      atggctttg aagaggcac aagcaccac atcggcccc 
      cgatacatc gaagtgtat cgcgcttcc ggcgaggac 
      ttcctctcg gtggctggg ggcaagagt tccgaagcc 
      caggccttc ctgtcccgg ggggctgcc gtcatcgtg 
      cgcatgcgg gggctgccc tacgactgc accgccaaa 
      caagtcttg gattttttc gcgagtggc gagaactcg 
      tgcaccgtt ttggacggc actgaaggc gtcctgttc 
      gtgcggaag cccgacggc cgagccact ggcgacgcg 
      ttcgttctg tttgcgagc gaatcggac gcgcctaaa 
      gctctctcg aaacatagg gaatgtata ggttctagg 
      tatatcgaa ttgttcagg tcgacgacg gccgaggtg 
      caacaggtg cttaacagg tcgctggaa gcgcggact 
      tacgagccg gaaccgttg cccccgtta ttaccgcac 
      atgccttta ttgcctcaa cattatatt acgtctggg 
      accaaaaag gaatgcatt aggttaaga ggactgcct 
      tacgaagct caagtcgaa cacatccta gagtttctc 
      ggagaatat gccaaaaat attatattc caaggggtg 
      catatggtc tacaatgca cagggccag cccagcggc 
      gaggccttc atccagatg gactccgag cagtccgcc 
      tgcataacc gcccagcag aagcaccac cgctacatg 
      acattcggc aaaaagcag cgctacatc gaagtattc 
      caatgctcc ggcgaggac atgaatctc gtactgacc 
      ggcggcatt cccgccccc gtctcgccc gcaaaggcc 
      acccccgcg ctactcagt cccggtatg ttaccttca 
      attgcgcct cttccacct actaatatc cccccgccg 
      cccccgcca aacacctcg gtcaaccaa atatcccca 
      aacatcaca aactgggac aaccaggcc cttttcgcc 
      caacagcag gcccagatc atagcccag caaaacctg 
      atcgcgcgc caaaaccaa gcgcaagcc cagaacgaa 
      atgctccta atgaaccag attgcgcaa cacaatctg 
      gccgtactc aaccaaaat gtgggccca gtgtcccca 
      aacagcccc aattcggtg ctgaaaaac acgccgatg 
      atgccgcat ttgatcgcg cagcccccg tttgtcatg 
      ctacccccg aggatggcc cccactttg gggctcccc 
      aggccccag ttggcctac ccccagcat ttgctgccc 
      gggggcgcg catggggtt tttccggtc atgccccac 
      gttgcggtt aaacgcagc tatggggac gcgttcaat 
      gagcaagcc gccccagcc aaaagggcc ttccatgca 
      caaggggcc ttgcccgtc taccaacac ttctaccct 
      aacatgtag
    mRNA sequence
    • >TC009268 RA
      tagctaaaa aagtgctgc aatacaagt ggctatttt 
      tgaaccagt ttagtgatt tccaatgat ctttcgcga 
      aatacttaa ctaaacacc gacctgaaa gcctgaaaa 
      agaaaaata gaaataggt attttaagg cttgccaac 
      ctgaaaaca aaaaagaca accaattgc agccaccag 
      tcattctga tgtgcactt tagaacatt ttgcggaaa 
      aactgatgg gaatttatg agtggcata taaacgcag 
      ctttgtgat gctctaggt gctcagtct ttatgacat 
      ccataaact aggtgatgc cttgaaaag tcaacacca 
      atgcaccaa taaatagac tgcggtcag tttggcacc 
      ggagtggag atttcgatg ctcgatcac atcacatta 
      aatgaattt ttgcttagt atttgtcga attaggtta 
      tgttcgctt ccccattcg ccatatgct ttcgccagt 
      cgacttgat aaaacgcta atcactcgt cttgtactc 
      gcagcatca attcttgtt gcctgaaag tgttgctca 
      tcacaatga ccattggca caagcaagc gaaaatgtg 
      cagaacatg cggcaaccg accaaagtt atctaatta 
      tataaccat agaatatgc ggctcgaat ccagttacc 
      ttgcgttag actgtacat acccttctc cacgattgg 
      ctcacggct caggtagaa ccagtctac gcggcccct 
      tcatacgta cacgggaaa attgaggct acgagttac 
      ctgcacgac aaaattact cgaaacttc caaagttcg 
      gcgactgtt aaaccaaac ccgtcgtca attgcgtag 
      aaggctgtg aacgcgttt tgtcattaa tttttcaaa 
      caattgccg atcgtcgac aacctgttg atcgcagtg 
      gccaggtgt gtagtgaca gtgactgtt ttagtgacg 
      ccaactgcg aaatcgcca aaaatcgca ctgtgatcg 
      aaatcccta ggttagagc gaatgtctg tgatagcat 
      ctccggaga taagatagc cccaggtgc cccacgttc 
      cccgaaatc caacaggtg tcacgtggc aacatgtcg 
      ggctggtga tggtgtgct tcagagcca ggatgaggt 
      gctgaggcc cctttggtg gacaaatga gcattaaag 
      gcctcaaca tgatcgggc cccaccatg tttgcgtcc 
      tgtacgtgg ccaccgcgg ggctccagg gctccctca 
      tgggctccg acgagcagg aggtggtgc tcctcatct 
      atgtcatcg tcgacgtcg tccagaata agattgtgg 
      gcgtgcagc agtacccaa tcaggccgc aaaccgccg 
      acatcaacg aaaatatcc tatcggaac agctcacat 
      ctgagtcgg ttttgaccg acgaactca taaaatcat 
      ctggaaggc aattggagg acgccctca acgaattcg 
      atgcctact gcaatagtt tacacatag acccgcaca 
      gtgccggct tcaggctcc taacagacg gccaattac 
      cgttaaggc agtgtctgt atccggaag catgtagga 
      aggaaattg atctgccgc gttattata gcacgttcc 
      atgacctca ggaaagaag ttgccaggt ttacgagaa 
      agacggaag aagtacccc aaagtgttg ccgatatgt 
      tagaatatt tacaaatta aacccgaag ttgagaatg 
      aattctaca agagggagg ctaaggata tggtcaata 
      ttgtacaaa ggctgattt cggaaggat acaggttcg 
      aggcgctcg aaacgataa acctgaccc tggagcccg 
      gaatatgtt ccaaagacg atgagattg acagcaact 
      gcgtggtga gggcgagag gacttccct ggcagagca 
      gcgatcagg acattgcca agtttttca gcggactca 
      atgtcgcca agggcggcg tagctctgt gcttgtcgg 
      cacaaggac gacggaacg gcgaagctt taataaaat 
      tcgagagtc aagagcacc gcgacatgg ctttgaaga 
      ggcacaagc accacatcg gcccccgat acatcgaag 
      tgtatcgcg cttccggcg aggacttcc tctcggtgg 
      ctgggggca agagttccg aagcccagg ccttcctgt 
      cccgggggg ctgccgtca tcgtgcgca tgcgggggc 
      tgccctacg actgcaccg ccaaacaag tcttggatt 
      ttttcgcga gtggcgaga actcgtgca ccgttttgg 
      acggcactg aaggcgtcc tgttcgtgc ggaagcccg 
      acggccgag ccactggcg acgcgttcg ttctgtttg 
      cgagcgaat cggacgcgc ctaaagctc tctcgaaac 
      atagggaat gtataggtt ctaggtata tcgaattgt 
      tcaggtcga cgacggccg aggtgcaac aggtgctta 
      acaggtcgc tggaagcgc ggacttacg agccggaac 
      cgttgcccc cgttattac cgcacatgc ctttattgc 
      ctcaacatt atattacgt ctgggacca aaaaggaat 
      gcattaggt taagaggac tgccttacg aagctcaag 
      tcgaacaca tcctagagt ttctcggag aatatgcca 
      aaaatatta tattccaag gggtgcata tggtctaca 
      atgcacagg gccagccca gcggcgagg ccttcatcc 
      agatggact ccgagcagt ccgcctgca taaccgccc 
      agcagaagc accaccgct acatgacat tcggcaaaa 
      agcagcgct acatcgaag tattccaat gctccggcg 
      aggacatga atctcgtac tgaccggcg gcattcccg 
      cccccgtct cgcccgcaa aggccaccc ccgcgctac 
      tcagtcccg gtatgttac cttcaattg cgcctcttc 
      cacctacta atatccccc cgccgcccc cgccaaaca 
      cctcggtca accaaatat ccccaaaca tcacaaact 
      gggacaacc aggcccttt tcgcccaac agcaggccc 
      agatcatag cccagcaaa acctgatcg cgcgccaaa 
      accaagcgc aagcccaga acgaaatgc tcctaatga 
      accagattg cgcaacaca atctggccg tactcaacc 
      aaaatgtgg gcccagtgt ccccaaaca gccccaatt 
      cggtgctga aaaacacgc cgatgatgc cgcatttga 
      tcgcgcagc ccccgtttg tcatgctac ccccgagga 
      tggccccca ctttggggc tccccaggc cccagttgg 
      cctaccccc agcatttgc tgcccgggg gcgcgcatg 
      gggtttttc cggtcatgc cccacgttg cggttaaac 
      gcagctatg gggacgcgt tcaatgagc aagccgccc 
      cagccaaaa gggccttcc atgcacaag gggccttgc 
      ccgtctacc aacacttct accctaaca tgtagcaag 
      tacgggcgg aaagttttc gcaacttaa agtgcctta 
      aagtgacaa ataatttga aaaaaaaaa ggatatttt 
      ttacggttt attatactt tttttttct aaaacacga 
      ttttgaaaa tttttttta cctcgtcca gtggtactt 
      tttgatttt cacattcga actactgta tatcagtga 
      tatttattg gttatttta ttgttagtt taacagtta 
      agaataata gataaggaa atgacctaa ttttgacga 
      tttatagag ttagtacca aaaatcact ctcataaac 
      tttttgtac atttgtata tattacgaa ttggtactc 
      tgataaata aaaagtgcc tcaaaaaga catgtaatt 
      gataattac tggctttat tccgaaatc gggttttgc 
      cacaaagag taccatttt ctgtggtac tatttttcg 
      aatttctga acattatcg ttaatgtag gaatcgtga 
      ataattgat aagagaaaa acgaagctc acatttggt 
      tgtaagttt tttattctt gcgtatttg tataattac 
      ggattgaaa ctaaaatcg gataataaa ttatttgtt 
      ttattttta ataaataaa gcgtttcca tacaa
    • >TC009268 RB
      tagctaaaa aagtgctgc aatacaagt ggctatttt 
      tgaaccagt ttagtgatt tccaatgat ctttcgcga 
      aatacttaa ctaaacacc gacctgaaa gcctgaaaa 
      agaaaaata gaaataggt attttaagg cttgccaac 
      ctgaaaaca aaaaagaca accaattgc agccaccag 
      tcattctga tgtgcactt tagaacatt ttgcggaaa 
      aactgatgg gaatttatg agtggcata taaacgcag 
      ctttgtgat gctctaggt gctcagtct ttatgacat 
      ccataaact aggtgatgc cttgaaaag tcaacacca 
      atgcaccaa taaatagac tgcggtcag tttggcacc 
      ggagtggag atttcgatg ctcgatcac atcacatta 
      aatgaattt ttgcttagt atttgtcga attaggtta 
      tgttcgctt ccccattcg ccatatgct ttcgccagt 
      cgacttgat aaaacgcta atcactcgt cttgtactc 
      gcagcatca attcttgtt gcctgaaag tgttgctca 
      tcacaatga ccattggca caagcaagc gaaaatgtg 
      cagaacatg cggcaaccg accaaagtt atctaatta 
      tataaccat agaatatgc ggctcgaat ccagttacc 
      ttgcgttag actgtacat acccttctc cacgattgg 
      ctcacggct caggtagaa ccagtctac gcggcccct 
      tcatacgta cacgggaaa attgaggct acgagttac 
      ctgcacgac aaaattact cgaaacttc caaagttcg 
      gcgactgtt aaaccaaac ccgtcgtca attgcgtag 
      aaggctgtg aacgcgttt tgtcattaa tttttcaaa 
      caattgccg atcgtcgac aacctgttg atcgcagtg 
      gccaggtgt gtagtgaca gtgactgtt ttagtgacg 
      ccaactgcg aaatcgcca aaaatcgca ctgtgatcg 
      aaatcccta ggttagagc gaatgtctg tgatagcat 
      ctccggaga taagatagc cccaggtgc cccacgttc 
      cccgaaatc caacaggtg tcacgtggc aacatgtcg 
      ggctggtga tggtgtgct tcagagcca ggatgaggt 
      gctgaggcc cctttggtg gacaaatga gcattaaag 
      gcctcaaca tgatcgggc cccaccatg tttgcgtcc 
      tgtacgtgg ccaccgcgg ggctccagg gctccctca 
      tgggctccg acgagcagg aggtggtgc tcctcatct 
      atgtcatcg tcgacgtcg tccagaata agattgtgg 
      gcgtgcagc agtacccaa tcaggccgc aaaccgccg 
      acatcaacg aaaatatcc tatcggaac agctcacat 
      ctgagtcgg ttttgaccg acgaactca taaaatcat 
      ctggaaggc aattggagg acgccctca acgaattcg 
      atgcctact gcaatagtt tacacatag acccgcaca 
      gtgccggct tcaggctcc taacagacg gccaattac 
      cgttaaggc agtgtctgt atccggaag catgtagga 
      aggaaattg atctgccgc gttattata gcacgttcc 
      atgacctca ggaaagaag ttgccaggt ttacgagaa 
      agacggaag aagtacccc aaagtgttg ccgatatgt 
      tagaattcc gcactcgtc gtacgcttc agttttgcg 
      aatatttac ggctaacct acaaaccag atttacaaa 
      ttaaacccg aagttgaga atgaattct acaagaggg 
      aggctaagg atatggtca atattgtac aaaggctga 
      tttcggaag gatacaggt tcgaggcgc tcgaaacga 
      taaacctga ccctggagc ccggaatat gttccaaag 
      acgatgaga ttgacagca actgcgtgg tgagggcga 
      gaggacttc cctggcaga gcagcgatc aggacattg 
      ccaagtttt tcagcggac tcaatgtcg ccaagggcg 
      gcgtagctc tgtgcttgt cggcacaag gacgacgga 
      acggcgaag ctttaataa aattcgaga gtcaagagc 
      accgcgaca tggctttga agaggcaca agcaccaca 
      tcggccccc gatacatcg aagtgtatc gcgcttccg 
      gcgaggact tcctctcgg tggctgggg gcaagagtt 
      ccgaagccc aggccttcc tgtcccggg gggctgccg 
      tcatcgtgc gcatgcggg ggctgccct acgactgca 
      ccgccaaac aagtcttgg attttttcg cgagtggcg 
      agaactcgt gcaccgttt tggacggca ctgaaggcg 
      tcctgttcg tgcggaagc ccgacggcc gagccactg 
      gcgacgcgt tcgttctgt ttgcgagcg aatcggacg 
      cgcctaaag ctctctcga aacataggg aatgtatag 
      gttctaggt atatcgaat tgttcaggt cgacgacgg 
      ccgaggtgc aacaggtgc ttaacaggt cgctggaag 
      cgcggactt acgagccgg aaccgttgc ccccgttat 
      taccgcaca tgcctttat tgcctcaac attatatta 
      cgtctggga ccaaaaagg aatgcatta ggttaagag 
      gactgcctt acgaagctc aagtcgaac acatcctag 
      agtttctcg gagaatatg ccaaaaata ttatattcc 
      aaggggtgc atatggtct acaatgcac agggccagc 
      ccagcggcg aggccttca tccagatgg actccgagc 
      agtccgcct gcataaccg cccagcaga agcaccacc 
      gctacatga cattcggca aaaagcagc gctacatcg 
      aagtattcc aatgctccg gcgaggaca tgaatctcg 
      tactgaccg gcggcattc ccgcccccg tctcgcccg 
      caaaggcca cccccgcgc tactcagtc ccggtatgt 
      taccttcaa ttgcgcctc ttccaccta ctaatatcc 
      ccccgccgc ccccgccaa acacctcgg tcaaccaaa 
      tatccccaa acatcacaa actgggaca accaggccc 
      ttttcgccc aacagcagg cccagatca tagcccagc 
      aaaacctga tcgcgcgcc aaaaccaag cgcaagccc 
      agaacgaaa tgctcctaa tgaaccaga ttgcgcaac 
      acaatctgg ccgtactca accaaaatg tgggcccag 
      tgtccccaa acagcccca attcggtgc tgaaaaaca 
      cgccgatga tgccgcatt tgatcgcgc agcccccgt 
      ttgtcatgc tacccccga ggatggccc ccactttgg 
      ggctcccca ggccccagt tggcctacc cccagcatt 
      tgctgcccg ggggcgcgc atggggttt ttccggtca 
      tgccccacg ttgcggtta aacgcagct atggggacg 
      cgttcaatg agcaagccg ccccagcca aaagggcct 
      tccatgcac aaggggcct tgcccgtct accaacact 
      tctacccta acatgtagc aagtacggg cggaaagtt 
      ttcgcaact taaagtgcc ttaaagtga caaataatt 
      tgaaaaaaa aaaggatat tttttacgg tttattata 
      ctttttttt tctaaaaca cgattttga aaatttttt 
      ttacctcgt ccagtggta ctttttgat tttcacatt 
      cgaactact gtatatcag tgatattta ttggttatt 
      ttattgtta gtttaacag ttaagaata atagataag 
      gaaatgacc taattttga cgatttata gagttagta 
      ccaaaaatc actctcata aactttttg tacatttgt 
      atatattac gaattggta ctctgataa ataaaaagt 
      gcctcaaaa agacatgta attgataat tactggctt 
      tattccgaa atcgggttt tgccacaaa gagtaccat 
      tttctgtgg tactatttt tcgaatttc tgaacatta 
      tcgttaatg taggaatcg tgaataatt gataagaga 
      aaaacgaag ctcacattt ggttgtaag ttttttatt 
      cttgcgtat ttgtataat tacggattg aaactaaaa 
      tcggataat aaattattt gttttattt ttaataaat 
      aaagcgttt ccatacaa