Status
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Gene information
General information
TC009259 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2319266..2340154
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009259
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01516

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection

    Comment: died with pupal and adult features

    Adult morphology
    abdominal odoriferous glands partially colour darker - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009259 RA
      MSRSQGRIQ VPDDLREVL LEFTISYLL EQPGDVVNY 
AADYFSRLR ETRTTQIVF DQAGTTASS PDESTLSRE 
EEPPVGRFS GRRKSVFAE AYDPEEDED DGGKVVFPK 
SDEQRQRLA EAVRNILLF RALDKEQMQ EVLDAMFER 
VVKEGELVI KQGDDGDNF YVIQNGIFH ALVGEPGGE 
QKHIHTYEN SGSFGELAL LYNMPRAAT IKAQTDGSL 
WAMDRQTFR RILLKSAFK KRKMYETLI ESVPMLKTL 
QPYERMNLA DALVPKTYQ SGDRIIKQG DAADGMYFV 
EDGTVIISV LDDSGKEVE INRLGKGGY FGELALVTH 
RPRAASAYA EGDVKLAFL DVEAFERLL GPCMQLMKR 
NITDYEEQM LKIFGSKHN IRDIR
    • >TC009259 RB
      MSRSQGRIQ VPDDLREVL LEFTISYLL EQPGDVVNY 
AADYFSRLR ETRTTQIVF DQAGTTASS PDESTLSRE 
EGSAEKPPV GRFSGRRKS VFAEAYDPE EDEDDGGKV 
VFPKSDEQR QRLAEAVRN ILLFRALDK EQMQEVLDA 
MFERVVKEG ELVIKQGDD GDNFYVIQN GIFHALVGE 
PGGEQKHIH TYENSGSFG ELALLYNMP RAATIKAQT 
DGSLWAMDR QTFRRILLK SAFKKRKMY ETLIESVPM 
LKTLQPYER MNLADALVP KTYQSGDRI IKQGDAADG 
MYFVEDGTV IISVLDDSG KEVEINRLG KGGYFGELA 
LVTHRPRAA SAYAEGDVK LAFLDVEAF ERLLGPCMQ 
LMKRNITDY EEQMLKIFG SKHNIRDIR
    CDS sequence
    • >TC009259 RA
      atgagtcga agccaaggc cgcatccaa gtcccagat 
gatcttcgc gaggtcctt ctcgaattc accataagc 
tacctgctg gagcagccc ggagacgtc gtcaactac 
gcagcggat tatttcagc cgtttgagg gaaacccgg 
accactcag atcgtcttc gaccaggcc ggaaccaca 
gcatcgtcg ccagatgag tccacgctg tcgcgagag 
gaagaaccg ccggtgggc cgattttcg ggccgtcgc 
aagtccgtc tttgccgag gcttacgat cccgaagag 
gacgaagac gatggcgga aaagtcgtt tttccgaaa 
tcggacgaa cagcgtcaa agattggcg gaagcagtt 
cgcaatatt ttactgttc cgtgccctc gacaaagag 
cagatgcaa gaagtgttg gacgcaatg ttcgaaagg 
gtcgtgaaa gagggcgaa ctcgttatc aagcaaggc 
gatgacggc gataacttt tatgttata caaaatggc 
atatttcat gccctcgtt ggcgagccc ggcggcgaa 
cagaagcac attcacacg tacgagaac agcggcagt 
tttggggaa ttggccctt ttatataac atgcctcgt 
gcggcaacg atcaaagcc caaacagat ggctccctt 
tgggcgatg gatcgccaa acgttccga cgcattttg 
ctaaagtcg gcgttcaaa aaacgaaag atgtacgaa 
acgctcatt gagagcgta ccgatgtta aagacttta 
cagccgtac gaacgcatg aatcttgcc gatgccctt 
gtaccaaaa acctatcaa tcgggcgat agaataatc 
aaacagggc gatgccgcc gatggaatg tatttcgtc 
gaagacggc actgtcatt atcagcgtc ttggacgac 
agcggaaaa gaggtcgaa atcaatcgc ttagggaaa 
ggaggctac tttggcgag ttggccttg gtcacacat 
cggccgagg gcggcgtcg gcctacgcc gaaggcgac 
gtcaaactg gcattcttg gacgtggag gcgttcgag 
cgactcttg ggcccgtgc atgcagctg atgaagcgc 
aacatcacc gactacgaa gaacaaatg ctcaagatc 
ttcggctca aagcataac attcgggac atccgatga
    • >TC009259 RB
      atgagtcga agccaaggc cgcatccaa gtcccagat 
gatcttcgc gaggtcctt ctcgaattc accataagc 
tacctgctg gagcagccc ggagacgtc gtcaactac 
gcagcggat tatttcagc cgtttgagg gaaacccgg 
accactcag atcgtcttc gaccaggcc ggaaccaca 
gcatcgtcg ccagatgag tccacgctg tcgcgagag 
gaaggttcg gcagaaaaa ccgccggtg ggccgattt 
tcgggccgt cgcaagtcc gtctttgcc gaggcttac 
gatcccgaa gaggacgaa gacgatggc ggaaaagtc 
gtttttccg aaatcggac gaacagcgt caaagattg 
gcggaagca gttcgcaat attttactg ttccgtgcc 
ctcgacaaa gagcagatg caagaagtg ttggacgca 
atgttcgaa agggtcgtg aaagagggc gaactcgtt 
atcaagcaa ggcgatgac ggcgataac ttttatgtt 
atacaaaat ggcatattt catgccctc gttggcgag 
cccggcggc gaacagaag cacattcac acgtacgag 
aacagcggc agttttggg gaattggcc cttttatat 
aacatgcct cgtgcggca acgatcaaa gcccaaaca 
gatggctcc ctttgggcg atggatcgc caaacgttc 
cgacgcatt ttgctaaag tcggcgttc aaaaaacga 
aagatgtac gaaacgctc attgagagc gtaccgatg 
ttaaagact ttacagccg tacgaacgc atgaatctt 
gccgatgcc cttgtacca aaaacctat caatcgggc 
gatagaata atcaaacag ggcgatgcc gccgatgga 
atgtatttc gtcgaagac ggcactgtc attatcagc 
gtcttggac gacagcgga aaagaggtc gaaatcaat 
cgcttaggg aaaggaggc tactttggc gagttggcc 
ttggtcaca catcggccg agggcggcg tcggcctac 
gccgaaggc gacgtcaaa ctggcattc ttggacgtg 
gaggcgttc gagcgactc ttgggcccg tgcatgcag 
ctgatgaag cgcaacatc accgactac gaagaacaa 
atgctcaag atcttcggc tcaaagcat aacattcgg 
gacatccga tga
    mRNA sequence
    • >TC009259 RA
      caccacttg tgctccgtg cgccactga tgccggtca 
cgtagtttc cctcttcgc cgccagttt tccacctgt 
caccttttc gaagtgtca gtcgatcat taatcattg 
attatcgcg gccaagtgt cggtgcagt gacgccccg 
ctcgtggaa atgcagtga gaaggcccc cctgaggca 
gagaaacgc gtccgcagt tgtcggcga gtggttcag 
tggtggtgt taaatgcga gactcgacg cttgtgagc 
tgggggctc ccgtgaacg gcagccgag gtcttctgg 
cgacggtga caccgcgtt tttcagatc cacacgtcg 
ttgcgttat cgccttttg agatatgag tcgaagcca 
aggccgcat ccaagtccc agatgatct tcgcgaggt 
ccttctcga attcaccat aagctacct gctggagca 
gcccggaga cgtcgtcaa ctacgcagc ggattattt 
cagccgttt gagggaaac ccggaccac tcagatcgt 
cttcgacca ggccggaac cacagcatc gtcgccaga 
tgagtccac gctgtcgcg agaggaaga accgccggt 
gggccgatt ttcgggccg tcgcaagtc cgtctttgc 
cgaggctta cgatcccga agaggacga agacgatgg 
cggaaaagt cgtttttcc gaaatcgga cgaacagcg 
tcaaagatt ggcggaagc agttcgcaa tattttact 
gttccgtgc cctcgacaa agagcagat gcaagaagt 
gttggacgc aatgttcga aagggtcgt gaaagaggg 
cgaactcgt tatcaagca aggcgatga cggcgataa 
cttttatgt tatacaaaa tggcatatt tcatgccct 
cgttggcga gcccggcgg cgaacagaa gcacattca 
cacgtacga gaacagcgg cagttttgg ggaattggc 
ccttttata taacatgcc tcgtgcggc aacgatcaa 
agcccaaac agatggctc cctttgggc gatggatcg 
ccaaacgtt ccgacgcat tttgctaaa gtcggcgtt 
caaaaaacg aaagatgta cgaaacgct cattgagag 
cgtaccgat gttaaagac tttacagcc gtacgaacg 
catgaatct tgccgatgc ccttgtacc aaaaaccta 
tcaatcggg cgatagaat aatcaaaca gggcgatgc 
cgccgatgg aatgtattt cgtcgaaga cggcactgt 
cattatcag cgtcttgga cgacagcgg aaaagaggt 
cgaaatcaa tcgcttagg gaaaggagg ctactttgg 
cgagttggc cttggtcac acatcggcc gagggcggc 
gtcggccta cgccgaagg cgacgtcaa actggcatt 
cttggacgt ggaggcgtt cgagcgact cttgggccc 
gtgcatgca gctgatgaa gcgcaacat caccgacta 
cgaagaaca aatgctcaa gatcttcgg ctcaaagca 
taacattcg ggacatccg atgaacgga gccgacgac 
ttgagtcgc tcccgcccg ccgccttct ctgaacgat 
agtcgaaca aacaatccc atcccaatt tgtattaat 
ttaaaatta ttacatgtc gagtagtaa acaatcaac 
gtgtgtcta ccgtcacta aaattcatc atataatca 
tcggagtgc aacagttta ctagtcatg tgtatatat 
aataatgtt atgaaactc ctttttgtt cttgtttcg 
ttgttgttg ttgctactg ctggacggt gtgcggccc 
ttgcccttt tttcgtcta ggaaatgtg cgtgtgttt 
ggatgcaag tcgttcgag tcttctcaa gtgtcgttt 
tctccccct accagcaat atcacacat gtggcgaac 
ggtgtgcca aaagtcgta caaaagaca cgcaataac 
agtattgtc aattagagt aggttctgc cgattttag 
tattatttg gaacacgcc gaggctttt ggcacagtt 
cgttcggtt ttgcgttac tattgggtt tgatgttcg 
ctatatttt gaggctatt agcgactta tacgttggc 
agtaaccgg gcagttatt ggcaacgta tttgtgaca 
cgttccttt attttaatg tgttcgagt ttgttatag 
ttttacgga caccgtaag aagactaga gctccatgt 
gagtctttg ttaataatt gttaatcgt acaaaaagg 
ttaacacat cttctttat acaaacata tacatattt 
attacaatg ttatttcgt gtgttctac gccaggttt 
tgagcagtt gtataagtt tcttcgaac aatcagttt 
gtctgccgt ttttgaact acgccacgt agttcgaga 
acagccaga caattaata ataattaag gctgataaa 
ttataaata tacactaga tgggtcacg attgaacga 
tattaggcg aaatggagt atttatttt tatgtgacg 
ataaatgga attgagaga gaggtggaa ccggttagt 
tacctagtt atagtaact ttttaagca aagttactc 
atatcacat aagttcagg tgaaaggtc cttgtaaca 
aaacccgta aatttgtaa gttaataca gagtttttt 
atgttgtgg gtggggttt gttagactg tgtgcctcc 
cgtgttcac aaacaatca aatcaaatc aaaacacat 
tcattagga aacggtaca ttttagact agtttaaac 
aaagctaaa acaatggtc tattatgtg ccttaaggc 
acgcgagct tgttcgaag aaaattcgg attgtgatt 
ggtctagca agtcctacc ggcggaata atttagcac 
aattctaat atttaaatg tgtagatat atagctagg 
tgtattatg tagtggctt gtcagttca tattcactg 
tttcgagag aaggtagaa aacaggtcg gttcatcct 
atgtgttat ttgtcaaag ttgaatgtt gattgttta 
tcgtggtat gtgtttctg tcccgacca tcttttcat 
cggatcgtc aagatcgga tcacttatc gcagtccga 
ctttggcga tcgctactg taaggtgtc tctaagcac 
gaataatgc ctttttact tatcgtacc aaaaattag 
aatctcaat taagttatt tgtacacaa gagattaat 
tataagtgt tcgccatgt cacatgcca tattctaaa 
cactctgag ctgactcgc tcgtcctgt ctgagattc 
cttagtcat atgtactgc ttccaccat gatgactaa 
atgagctta ttaaat
    • >TC009259 RB
      caccacttg tgctccgtg cgccactga tgccggtca 
cgtagtttc cctcttcgc cgccagttt tccacctgt 
caccttttc gaagtgtca gtcgatcat taatcattg 
attatcgcg gccaagtgt cggtgcagt gacgccccg 
ctcgtggaa atgcagtga gaaggcccc cctgaggca 
gagaaacgc gtccgcagt tgtcggcga gtggttcag 
tggtggtgt taaatgcga gactcgacg cttgtgagc 
tgggggctc ccgtgaacg gcagccgag gtcttctgg 
cgacggtga caccgcgtt tttcagatc cacacgtcg 
ttgcgttat cgccttttg agatatgag tcgaagcca 
aggccgcat ccaagtccc agatgatct tcgcgaggt 
ccttctcga attcaccat aagctacct gctggagca 
gcccggaga cgtcgtcaa ctacgcagc ggattattt 
cagccgttt gagggaaac ccggaccac tcagatcgt 
cttcgacca ggccggaac cacagcatc gtcgccaga 
tgagtccac gctgtcgcg agaggaagg ttcggcaga 
aaaaccgcc ggtgggccg attttcggg ccgtcgcaa 
gtccgtctt tgccgaggc ttacgatcc cgaagagga 
cgaagacga tggcggaaa agtcgtttt tccgaaatc 
ggacgaaca gcgtcaaag attggcgga agcagttcg 
caatatttt actgttccg tgccctcga caaagagca 
gatgcaaga agtgttgga cgcaatgtt cgaaagggt 
cgtgaaaga gggcgaact cgttatcaa gcaaggcga 
tgacggcga taactttta tgttataca aaatggcat 
atttcatgc cctcgttgg cgagcccgg cggcgaaca 
gaagcacat tcacacgta cgagaacag cggcagttt 
tggggaatt ggccctttt atataacat gcctcgtgc 
ggcaacgat caaagccca aacagatgg ctccctttg 
ggcgatgga tcgccaaac gttccgacg cattttgct 
aaagtcggc gttcaaaaa acgaaagat gtacgaaac 
gctcattga gagcgtacc gatgttaaa gactttaca 
gccgtacga acgcatgaa tcttgccga tgcccttgt 
accaaaaac ctatcaatc gggcgatag aataatcaa 
acagggcga tgccgccga tggaatgta tttcgtcga 
agacggcac tgtcattat cagcgtctt ggacgacag 
cggaaaaga ggtcgaaat caatcgctt agggaaagg 
aggctactt tggcgagtt ggccttggt cacacatcg 
gccgagggc ggcgtcggc ctacgccga aggcgacgt 
caaactggc attcttgga cgtggaggc gttcgagcg 
actcttggg cccgtgcat gcagctgat gaagcgcaa 
catcaccga ctacgaaga acaaatgct caagatctt 
cggctcaaa gcataacat tcgggacat ccgatgaac 
ggagccgac gacttgagt cgctcccgc ccgccgcct 
tctctgaac gatagtcga acaaacaat cccatccca 
atttgtatt aatttaaaa ttattacat gtcgagtag 
taaacaatc aacgtgtgt ctaccgtca ctaaaattc 
atcatataa tcatcggag tgcaacagt ttactagtc 
atgtgtata tataataat gttatgaaa ctccttttt 
gttcttgtt tcgttgttg ttgttgcta ctgctggac 
ggtgtgcgg cccttgccc ttttttcgt ctaggaaat 
gtgcgtgtg tttggatgc aagtcgttc gagtcttct 
caagtgtcg ttttctccc cctaccagc aatatcaca 
catgtggcg aacggtgtg ccaaaagtc gtacaaaag 
acacgcaat aacagtatt gtcaattag agtaggttc 
tgccgattt tagtattat ttggaacac gccgaggct 
tttggcaca gttcgttcg gttttgcgt tactattgg 
gtttgatgt tcgctatat tttgaggct attagcgac 
ttatacgtt ggcagtaac cgggcagtt attggcaac 
gtatttgtg acacgttcc tttatttta atgtgttcg 
agtttgtta tagttttac ggacaccgt aagaagact 
agagctcca tgtgagtct ttgttaata attgttaat 
cgtacaaaa aggttaaca catcttctt tatacaaac 
atatacata tttattaca atgttattt cgtgtgttc 
tacgccagg ttttgagca gttgtataa gtttcttcg 
aacaatcag tttgtctgc cgtttttga actacgcca 
cgtagttcg agaacagcc agacaatta ataataatt 
aaggctgat aaattataa atatacact agatgggtc 
acgattgaa cgatattag gcgaaatgg agtatttat 
ttttatgtg acgataaat ggaattgag agagaggtg 
gaaccggtt agttaccta gttatagta actttttaa 
gcaaagtta ctcatatca cataagttc aggtgaaag 
gtccttgta acaaaaccc gtaaatttg taagttaat 
acagagttt tttatgttg tgggtgggg tttgttaga 
ctgtgtgcc tcccgtgtt cacaaacaa tcaaatcaa 
atcaaaaca cattcatta ggaaacggt acattttag 
actagttta aacaaagct aaaacaatg gtctattat 
gtgccttaa ggcacgcga gcttgttcg aagaaaatt 
cggattgtg attggtcta gcaagtcct accggcgga 
ataatttag cacaattct aatatttaa atgtgtaga 
tatatagct aggtgtatt atgtagtgg cttgtcagt 
tcatattca ctgtttcga gagaaggta gaaaacagg 
tcggttcat cctatgtgt tatttgtca aagttgaat 
gttgattgt ttatcgtgg tatgtgttt ctgtcccga 
ccatctttt catcggatc gtcaagatc ggatcactt 
atcgcagtc cgactttgg cgatcgcta ctgtaaggt 
gtctctaag cacgaataa tgccttttt acttatcgt 
accaaaaat tagaatctc aattaagtt atttgtaca 
caagagatt aattataag tgttcgcca tgtcacatg 
ccatattct aaacactct gagctgact cgctcgtcc 
tgtctgaga ttccttagt catatgtac tgcttccac 
catgatgac taaatgagc ttattaaat
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