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Gene information
General information
TC009259 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 237366..258254
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009259
    iBeetle screen iB_01516
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    Comment: died with pupal and adult features

    Adult morphology
    abdominal odoriferous glands partially colour darker - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009259 RA
      MSRSQGRIQ VPDDLREVL LEFTISYLL EQPGDVVNY 
      AADYFSRLR ETRTTQIVF DQAGTTASS PDESTLSRE 
      EEPPVGRFS GRRKSVFAE AYDPEEDED DGGKVVFPK 
      SDEQRQRLA EAVRNILLF RALDKEQMQ EVLDAMFER 
      VVKEGELVI KQGDDGDNF YVIQNGIFH ALVGEPGGE 
      QKHIHTYEN SGSFGELAL LYNMPRAAT IKAQTDGSL 
      WAMDRQTFR RILLKSAFK KRKMYETLI ESVPMLKTL 
      QPYERMNLA DALVPKTYQ SGDRIIKQG DAADGMYFV 
      EDGTVIISV LDDSGKEVE INRLGKGGY FGELALVTH 
      RPRAASAYA EGDVKLAFL DVEAFERLL GPCMQLMKR 
      NITDYEEQM LKIFGSKHN IRDIR
    • >TC009259 RB
      MSRSQGRIQ VPDDLREVL LEFTISYLL EQPGDVVNY 
      AADYFSRLR ETRTTQIVF DQAGTTASS PDESTLSRE 
      EGSAEKPPV GRFSGRRKS VFAEAYDPE EDEDDGGKV 
      VFPKSDEQR QRLAEAVRN ILLFRALDK EQMQEVLDA 
      MFERVVKEG ELVIKQGDD GDNFYVIQN GIFHALVGE 
      PGGEQKHIH TYENSGSFG ELALLYNMP RAATIKAQT 
      DGSLWAMDR QTFRRILLK SAFKKRKMY ETLIESVPM 
      LKTLQPYER MNLADALVP KTYQSGDRI IKQGDAADG 
      MYFVEDGTV IISVLDDSG KEVEINRLG KGGYFGELA 
      LVTHRPRAA SAYAEGDVK LAFLDVEAF ERLLGPCMQ 
      LMKRNITDY EEQMLKIFG SKHNIRDIR
    CDS sequence
    • >TC009259 RA
      atgagtcga agccaaggc cgcatccaa gtcccagat 
      gatcttcgc gaggtcctt ctcgaattc accataagc 
      tacctgctg gagcagccc ggagacgtc gtcaactac 
      gcagcggat tatttcagc cgtttgagg gaaacccgg 
      accactcag atcgtcttc gaccaggcc ggaaccaca 
      gcatcgtcg ccagatgag tccacgctg tcgcgagag 
      gaagaaccg ccggtgggc cgattttcg ggccgtcgc 
      aagtccgtc tttgccgag gcttacgat cccgaagag 
      gacgaagac gatggcgga aaagtcgtt tttccgaaa 
      tcggacgaa cagcgtcaa agattggcg gaagcagtt 
      cgcaatatt ttactgttc cgtgccctc gacaaagag 
      cagatgcaa gaagtgttg gacgcaatg ttcgaaagg 
      gtcgtgaaa gagggcgaa ctcgttatc aagcaaggc 
      gatgacggc gataacttt tatgttata caaaatggc 
      atatttcat gccctcgtt ggcgagccc ggcggcgaa 
      cagaagcac attcacacg tacgagaac agcggcagt 
      tttggggaa ttggccctt ttatataac atgcctcgt 
      gcggcaacg atcaaagcc caaacagat ggctccctt 
      tgggcgatg gatcgccaa acgttccga cgcattttg 
      ctaaagtcg gcgttcaaa aaacgaaag atgtacgaa 
      acgctcatt gagagcgta ccgatgtta aagacttta 
      cagccgtac gaacgcatg aatcttgcc gatgccctt 
      gtaccaaaa acctatcaa tcgggcgat agaataatc 
      aaacagggc gatgccgcc gatggaatg tatttcgtc 
      gaagacggc actgtcatt atcagcgtc ttggacgac 
      agcggaaaa gaggtcgaa atcaatcgc ttagggaaa 
      ggaggctac tttggcgag ttggccttg gtcacacat 
      cggccgagg gcggcgtcg gcctacgcc gaaggcgac 
      gtcaaactg gcattcttg gacgtggag gcgttcgag 
      cgactcttg ggcccgtgc atgcagctg atgaagcgc 
      aacatcacc gactacgaa gaacaaatg ctcaagatc 
      ttcggctca aagcataac attcgggac atccgatga
    • >TC009259 RB
      atgagtcga agccaaggc cgcatccaa gtcccagat 
      gatcttcgc gaggtcctt ctcgaattc accataagc 
      tacctgctg gagcagccc ggagacgtc gtcaactac 
      gcagcggat tatttcagc cgtttgagg gaaacccgg 
      accactcag atcgtcttc gaccaggcc ggaaccaca 
      gcatcgtcg ccagatgag tccacgctg tcgcgagag 
      gaaggttcg gcagaaaaa ccgccggtg ggccgattt 
      tcgggccgt cgcaagtcc gtctttgcc gaggcttac 
      gatcccgaa gaggacgaa gacgatggc ggaaaagtc 
      gtttttccg aaatcggac gaacagcgt caaagattg 
      gcggaagca gttcgcaat attttactg ttccgtgcc 
      ctcgacaaa gagcagatg caagaagtg ttggacgca 
      atgttcgaa agggtcgtg aaagagggc gaactcgtt 
      atcaagcaa ggcgatgac ggcgataac ttttatgtt 
      atacaaaat ggcatattt catgccctc gttggcgag 
      cccggcggc gaacagaag cacattcac acgtacgag 
      aacagcggc agttttggg gaattggcc cttttatat 
      aacatgcct cgtgcggca acgatcaaa gcccaaaca 
      gatggctcc ctttgggcg atggatcgc caaacgttc 
      cgacgcatt ttgctaaag tcggcgttc aaaaaacga 
      aagatgtac gaaacgctc attgagagc gtaccgatg 
      ttaaagact ttacagccg tacgaacgc atgaatctt 
      gccgatgcc cttgtacca aaaacctat caatcgggc 
      gatagaata atcaaacag ggcgatgcc gccgatgga 
      atgtatttc gtcgaagac ggcactgtc attatcagc 
      gtcttggac gacagcgga aaagaggtc gaaatcaat 
      cgcttaggg aaaggaggc tactttggc gagttggcc 
      ttggtcaca catcggccg agggcggcg tcggcctac 
      gccgaaggc gacgtcaaa ctggcattc ttggacgtg 
      gaggcgttc gagcgactc ttgggcccg tgcatgcag 
      ctgatgaag cgcaacatc accgactac gaagaacaa 
      atgctcaag atcttcggc tcaaagcat aacattcgg 
      gacatccga tga
    mRNA sequence
    • >TC009259 RA
      caccacttg tgctccgtg cgccactga tgccggtca 
      cgtagtttc cctcttcgc cgccagttt tccacctgt 
      caccttttc gaagtgtca gtcgatcat taatcattg 
      attatcgcg gccaagtgt cggtgcagt gacgccccg 
      ctcgtggaa atgcagtga gaaggcccc cctgaggca 
      gagaaacgc gtccgcagt tgtcggcga gtggttcag 
      tggtggtgt taaatgcga gactcgacg cttgtgagc 
      tgggggctc ccgtgaacg gcagccgag gtcttctgg 
      cgacggtga caccgcgtt tttcagatc cacacgtcg 
      ttgcgttat cgccttttg agatatgag tcgaagcca 
      aggccgcat ccaagtccc agatgatct tcgcgaggt 
      ccttctcga attcaccat aagctacct gctggagca 
      gcccggaga cgtcgtcaa ctacgcagc ggattattt 
      cagccgttt gagggaaac ccggaccac tcagatcgt 
      cttcgacca ggccggaac cacagcatc gtcgccaga 
      tgagtccac gctgtcgcg agaggaaga accgccggt 
      gggccgatt ttcgggccg tcgcaagtc cgtctttgc 
      cgaggctta cgatcccga agaggacga agacgatgg 
      cggaaaagt cgtttttcc gaaatcgga cgaacagcg 
      tcaaagatt ggcggaagc agttcgcaa tattttact 
      gttccgtgc cctcgacaa agagcagat gcaagaagt 
      gttggacgc aatgttcga aagggtcgt gaaagaggg 
      cgaactcgt tatcaagca aggcgatga cggcgataa 
      cttttatgt tatacaaaa tggcatatt tcatgccct 
      cgttggcga gcccggcgg cgaacagaa gcacattca 
      cacgtacga gaacagcgg cagttttgg ggaattggc 
      ccttttata taacatgcc tcgtgcggc aacgatcaa 
      agcccaaac agatggctc cctttgggc gatggatcg 
      ccaaacgtt ccgacgcat tttgctaaa gtcggcgtt 
      caaaaaacg aaagatgta cgaaacgct cattgagag 
      cgtaccgat gttaaagac tttacagcc gtacgaacg 
      catgaatct tgccgatgc ccttgtacc aaaaaccta 
      tcaatcggg cgatagaat aatcaaaca gggcgatgc 
      cgccgatgg aatgtattt cgtcgaaga cggcactgt 
      cattatcag cgtcttgga cgacagcgg aaaagaggt 
      cgaaatcaa tcgcttagg gaaaggagg ctactttgg 
      cgagttggc cttggtcac acatcggcc gagggcggc 
      gtcggccta cgccgaagg cgacgtcaa actggcatt 
      cttggacgt ggaggcgtt cgagcgact cttgggccc 
      gtgcatgca gctgatgaa gcgcaacat caccgacta 
      cgaagaaca aatgctcaa gatcttcgg ctcaaagca 
      taacattcg ggacatccg atgaacgga gccgacgac 
      ttgagtcgc tcccgcccg ccgccttct ctgaacgat 
      agtcgaaca aacaatccc atcccaatt tgtattaat 
      ttaaaatta ttacatgtc gagtagtaa acaatcaac 
      gtgtgtcta ccgtcacta aaattcatc atataatca 
      tcggagtgc aacagttta ctagtcatg tgtatatat 
      aataatgtt atgaaactc ctttttgtt cttgtttcg 
      ttgttgttg ttgctactg ctggacggt gtgcggccc 
      ttgcccttt tttcgtcta ggaaatgtg cgtgtgttt 
      ggatgcaag tcgttcgag tcttctcaa gtgtcgttt 
      tctccccct accagcaat atcacacat gtggcgaac 
      ggtgtgcca aaagtcgta caaaagaca cgcaataac 
      agtattgtc aattagagt aggttctgc cgattttag 
      tattatttg gaacacgcc gaggctttt ggcacagtt 
      cgttcggtt ttgcgttac tattgggtt tgatgttcg 
      ctatatttt gaggctatt agcgactta tacgttggc 
      agtaaccgg gcagttatt ggcaacgta tttgtgaca 
      cgttccttt attttaatg tgttcgagt ttgttatag 
      ttttacgga caccgtaag aagactaga gctccatgt 
      gagtctttg ttaataatt gttaatcgt acaaaaagg 
      ttaacacat cttctttat acaaacata tacatattt 
      attacaatg ttatttcgt gtgttctac gccaggttt 
      tgagcagtt gtataagtt tcttcgaac aatcagttt 
      gtctgccgt ttttgaact acgccacgt agttcgaga 
      acagccaga caattaata ataattaag gctgataaa 
      ttataaata tacactaga tgggtcacg attgaacga 
      tattaggcg aaatggagt atttatttt tatgtgacg 
      ataaatgga attgagaga gaggtggaa ccggttagt 
      tacctagtt atagtaact ttttaagca aagttactc 
      atatcacat aagttcagg tgaaaggtc cttgtaaca 
      aaacccgta aatttgtaa gttaataca gagtttttt 
      atgttgtgg gtggggttt gttagactg tgtgcctcc 
      cgtgttcac aaacaatca aatcaaatc aaaacacat 
      tcattagga aacggtaca ttttagact agtttaaac 
      aaagctaaa acaatggtc tattatgtg ccttaaggc 
      acgcgagct tgttcgaag aaaattcgg attgtgatt 
      ggtctagca agtcctacc ggcggaata atttagcac 
      aattctaat atttaaatg tgtagatat atagctagg 
      tgtattatg tagtggctt gtcagttca tattcactg 
      tttcgagag aaggtagaa aacaggtcg gttcatcct 
      atgtgttat ttgtcaaag ttgaatgtt gattgttta 
      tcgtggtat gtgtttctg tcccgacca tcttttcat 
      cggatcgtc aagatcgga tcacttatc gcagtccga 
      ctttggcga tcgctactg taaggtgtc tctaagcac 
      gaataatgc ctttttact tatcgtacc aaaaattag 
      aatctcaat taagttatt tgtacacaa gagattaat 
      tataagtgt tcgccatgt cacatgcca tattctaaa 
      cactctgag ctgactcgc tcgtcctgt ctgagattc 
      cttagtcat atgtactgc ttccaccat gatgactaa 
      atgagctta ttaaat
    • >TC009259 RB
      caccacttg tgctccgtg cgccactga tgccggtca 
      cgtagtttc cctcttcgc cgccagttt tccacctgt 
      caccttttc gaagtgtca gtcgatcat taatcattg 
      attatcgcg gccaagtgt cggtgcagt gacgccccg 
      ctcgtggaa atgcagtga gaaggcccc cctgaggca 
      gagaaacgc gtccgcagt tgtcggcga gtggttcag 
      tggtggtgt taaatgcga gactcgacg cttgtgagc 
      tgggggctc ccgtgaacg gcagccgag gtcttctgg 
      cgacggtga caccgcgtt tttcagatc cacacgtcg 
      ttgcgttat cgccttttg agatatgag tcgaagcca 
      aggccgcat ccaagtccc agatgatct tcgcgaggt 
      ccttctcga attcaccat aagctacct gctggagca 
      gcccggaga cgtcgtcaa ctacgcagc ggattattt 
      cagccgttt gagggaaac ccggaccac tcagatcgt 
      cttcgacca ggccggaac cacagcatc gtcgccaga 
      tgagtccac gctgtcgcg agaggaagg ttcggcaga 
      aaaaccgcc ggtgggccg attttcggg ccgtcgcaa 
      gtccgtctt tgccgaggc ttacgatcc cgaagagga 
      cgaagacga tggcggaaa agtcgtttt tccgaaatc 
      ggacgaaca gcgtcaaag attggcgga agcagttcg 
      caatatttt actgttccg tgccctcga caaagagca 
      gatgcaaga agtgttgga cgcaatgtt cgaaagggt 
      cgtgaaaga gggcgaact cgttatcaa gcaaggcga 
      tgacggcga taactttta tgttataca aaatggcat 
      atttcatgc cctcgttgg cgagcccgg cggcgaaca 
      gaagcacat tcacacgta cgagaacag cggcagttt 
      tggggaatt ggccctttt atataacat gcctcgtgc 
      ggcaacgat caaagccca aacagatgg ctccctttg 
      ggcgatgga tcgccaaac gttccgacg cattttgct 
      aaagtcggc gttcaaaaa acgaaagat gtacgaaac 
      gctcattga gagcgtacc gatgttaaa gactttaca 
      gccgtacga acgcatgaa tcttgccga tgcccttgt 
      accaaaaac ctatcaatc gggcgatag aataatcaa 
      acagggcga tgccgccga tggaatgta tttcgtcga 
      agacggcac tgtcattat cagcgtctt ggacgacag 
      cggaaaaga ggtcgaaat caatcgctt agggaaagg 
      aggctactt tggcgagtt ggccttggt cacacatcg 
      gccgagggc ggcgtcggc ctacgccga aggcgacgt 
      caaactggc attcttgga cgtggaggc gttcgagcg 
      actcttggg cccgtgcat gcagctgat gaagcgcaa 
      catcaccga ctacgaaga acaaatgct caagatctt 
      cggctcaaa gcataacat tcgggacat ccgatgaac 
      ggagccgac gacttgagt cgctcccgc ccgccgcct 
      tctctgaac gatagtcga acaaacaat cccatccca 
      atttgtatt aatttaaaa ttattacat gtcgagtag 
      taaacaatc aacgtgtgt ctaccgtca ctaaaattc 
      atcatataa tcatcggag tgcaacagt ttactagtc 
      atgtgtata tataataat gttatgaaa ctccttttt 
      gttcttgtt tcgttgttg ttgttgcta ctgctggac 
      ggtgtgcgg cccttgccc ttttttcgt ctaggaaat 
      gtgcgtgtg tttggatgc aagtcgttc gagtcttct 
      caagtgtcg ttttctccc cctaccagc aatatcaca 
      catgtggcg aacggtgtg ccaaaagtc gtacaaaag 
      acacgcaat aacagtatt gtcaattag agtaggttc 
      tgccgattt tagtattat ttggaacac gccgaggct 
      tttggcaca gttcgttcg gttttgcgt tactattgg 
      gtttgatgt tcgctatat tttgaggct attagcgac 
      ttatacgtt ggcagtaac cgggcagtt attggcaac 
      gtatttgtg acacgttcc tttatttta atgtgttcg 
      agtttgtta tagttttac ggacaccgt aagaagact 
      agagctcca tgtgagtct ttgttaata attgttaat 
      cgtacaaaa aggttaaca catcttctt tatacaaac 
      atatacata tttattaca atgttattt cgtgtgttc 
      tacgccagg ttttgagca gttgtataa gtttcttcg 
      aacaatcag tttgtctgc cgtttttga actacgcca 
      cgtagttcg agaacagcc agacaatta ataataatt 
      aaggctgat aaattataa atatacact agatgggtc 
      acgattgaa cgatattag gcgaaatgg agtatttat 
      ttttatgtg acgataaat ggaattgag agagaggtg 
      gaaccggtt agttaccta gttatagta actttttaa 
      gcaaagtta ctcatatca cataagttc aggtgaaag 
      gtccttgta acaaaaccc gtaaatttg taagttaat 
      acagagttt tttatgttg tgggtgggg tttgttaga 
      ctgtgtgcc tcccgtgtt cacaaacaa tcaaatcaa 
      atcaaaaca cattcatta ggaaacggt acattttag 
      actagttta aacaaagct aaaacaatg gtctattat 
      gtgccttaa ggcacgcga gcttgttcg aagaaaatt 
      cggattgtg attggtcta gcaagtcct accggcgga 
      ataatttag cacaattct aatatttaa atgtgtaga 
      tatatagct aggtgtatt atgtagtgg cttgtcagt 
      tcatattca ctgtttcga gagaaggta gaaaacagg 
      tcggttcat cctatgtgt tatttgtca aagttgaat 
      gttgattgt ttatcgtgg tatgtgttt ctgtcccga 
      ccatctttt catcggatc gtcaagatc ggatcactt 
      atcgcagtc cgactttgg cgatcgcta ctgtaaggt 
      gtctctaag cacgaataa tgccttttt acttatcgt 
      accaaaaat tagaatctc aattaagtt atttgtaca 
      caagagatt aattataag tgttcgcca tgtcacatg 
      ccatattct aaacactct gagctgact cgctcgtcc 
      tgtctgaga ttccttagt catatgtac tgcttccac 
      catgatgac taaatgagc ttattaaat