General information | |||
TC009259 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 2319266..2340154 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC009259 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01516 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)remarks: bad quality of preparation,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
Comment: died with pupal and adult features |
- >TC009259 RA
MSRSQGRIQ VPDDLREVL LEFTISYLL EQPGDVVNY AADYFSRLR ETRTTQIVF DQAGTTASS PDESTLSRE EEPPVGRFS GRRKSVFAE AYDPEEDED DGGKVVFPK SDEQRQRLA EAVRNILLF RALDKEQMQ EVLDAMFER VVKEGELVI KQGDDGDNF YVIQNGIFH ALVGEPGGE QKHIHTYEN SGSFGELAL LYNMPRAAT IKAQTDGSL WAMDRQTFR RILLKSAFK KRKMYETLI ESVPMLKTL QPYERMNLA DALVPKTYQ SGDRIIKQG DAADGMYFV EDGTVIISV LDDSGKEVE INRLGKGGY FGELALVTH RPRAASAYA EGDVKLAFL DVEAFERLL GPCMQLMKR NITDYEEQM LKIFGSKHN IRDIR
- >TC009259 RB
MSRSQGRIQ VPDDLREVL LEFTISYLL EQPGDVVNY AADYFSRLR ETRTTQIVF DQAGTTASS PDESTLSRE EGSAEKPPV GRFSGRRKS VFAEAYDPE EDEDDGGKV VFPKSDEQR QRLAEAVRN ILLFRALDK EQMQEVLDA MFERVVKEG ELVIKQGDD GDNFYVIQN GIFHALVGE PGGEQKHIH TYENSGSFG ELALLYNMP RAATIKAQT DGSLWAMDR QTFRRILLK SAFKKRKMY ETLIESVPM LKTLQPYER MNLADALVP KTYQSGDRI IKQGDAADG MYFVEDGTV IISVLDDSG KEVEINRLG KGGYFGELA LVTHRPRAA SAYAEGDVK LAFLDVEAF ERLLGPCMQ LMKRNITDY EEQMLKIFG SKHNIRDIR
- >TC009259 RA
atgagtcga agccaaggc cgcatccaa gtcccagat gatcttcgc gaggtcctt ctcgaattc accataagc tacctgctg gagcagccc ggagacgtc gtcaactac gcagcggat tatttcagc cgtttgagg gaaacccgg accactcag atcgtcttc gaccaggcc ggaaccaca gcatcgtcg ccagatgag tccacgctg tcgcgagag gaagaaccg ccggtgggc cgattttcg ggccgtcgc aagtccgtc tttgccgag gcttacgat cccgaagag gacgaagac gatggcgga aaagtcgtt tttccgaaa tcggacgaa cagcgtcaa agattggcg gaagcagtt cgcaatatt ttactgttc cgtgccctc gacaaagag cagatgcaa gaagtgttg gacgcaatg ttcgaaagg gtcgtgaaa gagggcgaa ctcgttatc aagcaaggc gatgacggc gataacttt tatgttata caaaatggc atatttcat gccctcgtt ggcgagccc ggcggcgaa cagaagcac attcacacg tacgagaac agcggcagt tttggggaa ttggccctt ttatataac atgcctcgt gcggcaacg atcaaagcc caaacagat ggctccctt tgggcgatg gatcgccaa acgttccga cgcattttg ctaaagtcg gcgttcaaa aaacgaaag atgtacgaa acgctcatt gagagcgta ccgatgtta aagacttta cagccgtac gaacgcatg aatcttgcc gatgccctt gtaccaaaa acctatcaa tcgggcgat agaataatc aaacagggc gatgccgcc gatggaatg tatttcgtc gaagacggc actgtcatt atcagcgtc ttggacgac agcggaaaa gaggtcgaa atcaatcgc ttagggaaa ggaggctac tttggcgag ttggccttg gtcacacat cggccgagg gcggcgtcg gcctacgcc gaaggcgac gtcaaactg gcattcttg gacgtggag gcgttcgag cgactcttg ggcccgtgc atgcagctg atgaagcgc aacatcacc gactacgaa gaacaaatg ctcaagatc ttcggctca aagcataac attcgggac atccgatga
- >TC009259 RB
atgagtcga agccaaggc cgcatccaa gtcccagat gatcttcgc gaggtcctt ctcgaattc accataagc tacctgctg gagcagccc ggagacgtc gtcaactac gcagcggat tatttcagc cgtttgagg gaaacccgg accactcag atcgtcttc gaccaggcc ggaaccaca gcatcgtcg ccagatgag tccacgctg tcgcgagag gaaggttcg gcagaaaaa ccgccggtg ggccgattt tcgggccgt cgcaagtcc gtctttgcc gaggcttac gatcccgaa gaggacgaa gacgatggc ggaaaagtc gtttttccg aaatcggac gaacagcgt caaagattg gcggaagca gttcgcaat attttactg ttccgtgcc ctcgacaaa gagcagatg caagaagtg ttggacgca atgttcgaa agggtcgtg aaagagggc gaactcgtt atcaagcaa ggcgatgac ggcgataac ttttatgtt atacaaaat ggcatattt catgccctc gttggcgag cccggcggc gaacagaag cacattcac acgtacgag aacagcggc agttttggg gaattggcc cttttatat aacatgcct cgtgcggca acgatcaaa gcccaaaca gatggctcc ctttgggcg atggatcgc caaacgttc cgacgcatt ttgctaaag tcggcgttc aaaaaacga aagatgtac gaaacgctc attgagagc gtaccgatg ttaaagact ttacagccg tacgaacgc atgaatctt gccgatgcc cttgtacca aaaacctat caatcgggc gatagaata atcaaacag ggcgatgcc gccgatgga atgtatttc gtcgaagac ggcactgtc attatcagc gtcttggac gacagcgga aaagaggtc gaaatcaat cgcttaggg aaaggaggc tactttggc gagttggcc ttggtcaca catcggccg agggcggcg tcggcctac gccgaaggc gacgtcaaa ctggcattc ttggacgtg gaggcgttc gagcgactc ttgggcccg tgcatgcag ctgatgaag cgcaacatc accgactac gaagaacaa atgctcaag atcttcggc tcaaagcat aacattcgg gacatccga tga
- >TC009259 RA
caccacttg tgctccgtg cgccactga tgccggtca cgtagtttc cctcttcgc cgccagttt tccacctgt caccttttc gaagtgtca gtcgatcat taatcattg attatcgcg gccaagtgt cggtgcagt gacgccccg ctcgtggaa atgcagtga gaaggcccc cctgaggca gagaaacgc gtccgcagt tgtcggcga gtggttcag tggtggtgt taaatgcga gactcgacg cttgtgagc tgggggctc ccgtgaacg gcagccgag gtcttctgg cgacggtga caccgcgtt tttcagatc cacacgtcg ttgcgttat cgccttttg agatatgag tcgaagcca aggccgcat ccaagtccc agatgatct tcgcgaggt ccttctcga attcaccat aagctacct gctggagca gcccggaga cgtcgtcaa ctacgcagc ggattattt cagccgttt gagggaaac ccggaccac tcagatcgt cttcgacca ggccggaac cacagcatc gtcgccaga tgagtccac gctgtcgcg agaggaaga accgccggt gggccgatt ttcgggccg tcgcaagtc cgtctttgc cgaggctta cgatcccga agaggacga agacgatgg cggaaaagt cgtttttcc gaaatcgga cgaacagcg tcaaagatt ggcggaagc agttcgcaa tattttact gttccgtgc cctcgacaa agagcagat gcaagaagt gttggacgc aatgttcga aagggtcgt gaaagaggg cgaactcgt tatcaagca aggcgatga cggcgataa cttttatgt tatacaaaa tggcatatt tcatgccct cgttggcga gcccggcgg cgaacagaa gcacattca cacgtacga gaacagcgg cagttttgg ggaattggc ccttttata taacatgcc tcgtgcggc aacgatcaa agcccaaac agatggctc cctttgggc gatggatcg ccaaacgtt ccgacgcat tttgctaaa gtcggcgtt caaaaaacg aaagatgta cgaaacgct cattgagag cgtaccgat gttaaagac tttacagcc gtacgaacg catgaatct tgccgatgc ccttgtacc aaaaaccta tcaatcggg cgatagaat aatcaaaca gggcgatgc cgccgatgg aatgtattt cgtcgaaga cggcactgt cattatcag cgtcttgga cgacagcgg aaaagaggt cgaaatcaa tcgcttagg gaaaggagg ctactttgg cgagttggc cttggtcac acatcggcc gagggcggc gtcggccta cgccgaagg cgacgtcaa actggcatt cttggacgt ggaggcgtt cgagcgact cttgggccc gtgcatgca gctgatgaa gcgcaacat caccgacta cgaagaaca aatgctcaa gatcttcgg ctcaaagca taacattcg ggacatccg atgaacgga gccgacgac ttgagtcgc tcccgcccg ccgccttct ctgaacgat agtcgaaca aacaatccc atcccaatt tgtattaat ttaaaatta ttacatgtc gagtagtaa acaatcaac gtgtgtcta ccgtcacta aaattcatc atataatca tcggagtgc aacagttta ctagtcatg tgtatatat aataatgtt atgaaactc ctttttgtt cttgtttcg ttgttgttg ttgctactg ctggacggt gtgcggccc ttgcccttt tttcgtcta ggaaatgtg cgtgtgttt ggatgcaag tcgttcgag tcttctcaa gtgtcgttt tctccccct accagcaat atcacacat gtggcgaac ggtgtgcca aaagtcgta caaaagaca cgcaataac agtattgtc aattagagt aggttctgc cgattttag tattatttg gaacacgcc gaggctttt ggcacagtt cgttcggtt ttgcgttac tattgggtt tgatgttcg ctatatttt gaggctatt agcgactta tacgttggc agtaaccgg gcagttatt ggcaacgta tttgtgaca cgttccttt attttaatg tgttcgagt ttgttatag ttttacgga caccgtaag aagactaga gctccatgt gagtctttg ttaataatt gttaatcgt acaaaaagg ttaacacat cttctttat acaaacata tacatattt attacaatg ttatttcgt gtgttctac gccaggttt tgagcagtt gtataagtt tcttcgaac aatcagttt gtctgccgt ttttgaact acgccacgt agttcgaga acagccaga caattaata ataattaag gctgataaa ttataaata tacactaga tgggtcacg attgaacga tattaggcg aaatggagt atttatttt tatgtgacg ataaatgga attgagaga gaggtggaa ccggttagt tacctagtt atagtaact ttttaagca aagttactc atatcacat aagttcagg tgaaaggtc cttgtaaca aaacccgta aatttgtaa gttaataca gagtttttt atgttgtgg gtggggttt gttagactg tgtgcctcc cgtgttcac aaacaatca aatcaaatc aaaacacat tcattagga aacggtaca ttttagact agtttaaac aaagctaaa acaatggtc tattatgtg ccttaaggc acgcgagct tgttcgaag aaaattcgg attgtgatt ggtctagca agtcctacc ggcggaata atttagcac aattctaat atttaaatg tgtagatat atagctagg tgtattatg tagtggctt gtcagttca tattcactg tttcgagag aaggtagaa aacaggtcg gttcatcct atgtgttat ttgtcaaag ttgaatgtt gattgttta tcgtggtat gtgtttctg tcccgacca tcttttcat cggatcgtc aagatcgga tcacttatc gcagtccga ctttggcga tcgctactg taaggtgtc tctaagcac gaataatgc ctttttact tatcgtacc aaaaattag aatctcaat taagttatt tgtacacaa gagattaat tataagtgt tcgccatgt cacatgcca tattctaaa cactctgag ctgactcgc tcgtcctgt ctgagattc cttagtcat atgtactgc ttccaccat gatgactaa atgagctta ttaaat
- >TC009259 RB
caccacttg tgctccgtg cgccactga tgccggtca cgtagtttc cctcttcgc cgccagttt tccacctgt caccttttc gaagtgtca gtcgatcat taatcattg attatcgcg gccaagtgt cggtgcagt gacgccccg ctcgtggaa atgcagtga gaaggcccc cctgaggca gagaaacgc gtccgcagt tgtcggcga gtggttcag tggtggtgt taaatgcga gactcgacg cttgtgagc tgggggctc ccgtgaacg gcagccgag gtcttctgg cgacggtga caccgcgtt tttcagatc cacacgtcg ttgcgttat cgccttttg agatatgag tcgaagcca aggccgcat ccaagtccc agatgatct tcgcgaggt ccttctcga attcaccat aagctacct gctggagca gcccggaga cgtcgtcaa ctacgcagc ggattattt cagccgttt gagggaaac ccggaccac tcagatcgt cttcgacca ggccggaac cacagcatc gtcgccaga tgagtccac gctgtcgcg agaggaagg ttcggcaga aaaaccgcc ggtgggccg attttcggg ccgtcgcaa gtccgtctt tgccgaggc ttacgatcc cgaagagga cgaagacga tggcggaaa agtcgtttt tccgaaatc ggacgaaca gcgtcaaag attggcgga agcagttcg caatatttt actgttccg tgccctcga caaagagca gatgcaaga agtgttgga cgcaatgtt cgaaagggt cgtgaaaga gggcgaact cgttatcaa gcaaggcga tgacggcga taactttta tgttataca aaatggcat atttcatgc cctcgttgg cgagcccgg cggcgaaca gaagcacat tcacacgta cgagaacag cggcagttt tggggaatt ggccctttt atataacat gcctcgtgc ggcaacgat caaagccca aacagatgg ctccctttg ggcgatgga tcgccaaac gttccgacg cattttgct aaagtcggc gttcaaaaa acgaaagat gtacgaaac gctcattga gagcgtacc gatgttaaa gactttaca gccgtacga acgcatgaa tcttgccga tgcccttgt accaaaaac ctatcaatc gggcgatag aataatcaa acagggcga tgccgccga tggaatgta tttcgtcga agacggcac tgtcattat cagcgtctt ggacgacag cggaaaaga ggtcgaaat caatcgctt agggaaagg aggctactt tggcgagtt ggccttggt cacacatcg gccgagggc ggcgtcggc ctacgccga aggcgacgt caaactggc attcttgga cgtggaggc gttcgagcg actcttggg cccgtgcat gcagctgat gaagcgcaa catcaccga ctacgaaga acaaatgct caagatctt cggctcaaa gcataacat tcgggacat ccgatgaac ggagccgac gacttgagt cgctcccgc ccgccgcct tctctgaac gatagtcga acaaacaat cccatccca atttgtatt aatttaaaa ttattacat gtcgagtag taaacaatc aacgtgtgt ctaccgtca ctaaaattc atcatataa tcatcggag tgcaacagt ttactagtc atgtgtata tataataat gttatgaaa ctccttttt gttcttgtt tcgttgttg ttgttgcta ctgctggac ggtgtgcgg cccttgccc ttttttcgt ctaggaaat gtgcgtgtg tttggatgc aagtcgttc gagtcttct caagtgtcg ttttctccc cctaccagc aatatcaca catgtggcg aacggtgtg ccaaaagtc gtacaaaag acacgcaat aacagtatt gtcaattag agtaggttc tgccgattt tagtattat ttggaacac gccgaggct tttggcaca gttcgttcg gttttgcgt tactattgg gtttgatgt tcgctatat tttgaggct attagcgac ttatacgtt ggcagtaac cgggcagtt attggcaac gtatttgtg acacgttcc tttatttta atgtgttcg agtttgtta tagttttac ggacaccgt aagaagact agagctcca tgtgagtct ttgttaata attgttaat cgtacaaaa aggttaaca catcttctt tatacaaac atatacata tttattaca atgttattt cgtgtgttc tacgccagg ttttgagca gttgtataa gtttcttcg aacaatcag tttgtctgc cgtttttga actacgcca cgtagttcg agaacagcc agacaatta ataataatt aaggctgat aaattataa atatacact agatgggtc acgattgaa cgatattag gcgaaatgg agtatttat ttttatgtg acgataaat ggaattgag agagaggtg gaaccggtt agttaccta gttatagta actttttaa gcaaagtta ctcatatca cataagttc aggtgaaag gtccttgta acaaaaccc gtaaatttg taagttaat acagagttt tttatgttg tgggtgggg tttgttaga ctgtgtgcc tcccgtgtt cacaaacaa tcaaatcaa atcaaaaca cattcatta ggaaacggt acattttag actagttta aacaaagct aaaacaatg gtctattat gtgccttaa ggcacgcga gcttgttcg aagaaaatt cggattgtg attggtcta gcaagtcct accggcgga ataatttag cacaattct aatatttaa atgtgtaga tatatagct aggtgtatt atgtagtgg cttgtcagt tcatattca ctgtttcga gagaaggta gaaaacagg tcggttcat cctatgtgt tatttgtca aagttgaat gttgattgt ttatcgtgg tatgtgttt ctgtcccga ccatctttt catcggatc gtcaagatc ggatcactt atcgcagtc cgactttgg cgatcgcta ctgtaaggt gtctctaag cacgaataa tgccttttt acttatcgt accaaaaat tagaatctc aattaagtt atttgtaca caagagatt aattataag tgttcgcca tgtcacatg ccatattct aaacactct gagctgact cgctcgtcc tgtctgaga ttccttagt catatgtac tgcttccac catgatgac taaatgagc ttattaaat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
