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Gene information
General information
TC009257 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 265959..267436
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009257
    iBeetle screen iB_01515
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Adult morphology
    adult cuticularisation sticky - Number of animals showing the phenotype: 5


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009257 RA
      MRRGMPCWY KKDDTGLNA FNDAILLEH GIYTLLKRH 
      FSQHHCYVP TMELFHDVT LKTSMGQAL DCLCNKDGK 
      PNLELFTMN KYNSIVKYK TAYYTFQLP VALAMYMAN 
      LYDPEMHRQ AKTILMEMG LFFQIQDDF LDCFGDPEV 
      TGKKGNDIR EGKCSWLAV VALQRANPT QRKIMEEYY 
      GRPDPEAVR IIRNLYEEL SLPNTYAIY EEESFNIIR 
      THIQQISKG LPHKLFFKI MEKIYRRDC
    CDS sequence
    • >TC009257 RA
      atgcgacgt ggaatgccc tgttggtac aagaaggac 
      gacaccggt ttaaatgcc ttcaacgat gccattctt 
      ctcgaacac ggcatttat accttgctg aaaaggcat 
      ttctcgcaa caccattgc tacgttccc accatggag 
      ctcttccat gatgtcacg ctcaaaact tcaatggga 
      caagctcta gattgtttg tgtaataaa gacggaaaa 
      ccgaatttg gaactcttc actatgaat aaatacaac 
      tcgattgtt aaatacaag actgcttat tacacgttc 
      caactaccg gtggctcta gccatgtat atggcgaat 
      ttgtacgac ccagaaatg caccgacag gccaaaacc 
      atcttgatg gaaatgggg cttttcttc caaattcag 
      gacgatttc ctggactgc ttcggcgac cctgaggtg 
      acgggcaag aagggcaac gacatccgc gagggcaag 
      tgctcctgg ctggctgtg gtggccctc caaagggcc 
      aaccccacc cagcgaaaa ataatggag gagtattac 
      ggccgtccc gaccctgaa gcagtccga atcatccga 
      aacctgtac gaagagttg agtttgcca aacacttat 
      gccatctat gaggaggag agcttcaac atcatccgt 
      acacacatc cagcaaatt tccaaagga cttccacac 
      aagttgttt ttcaaaatt atggagaaa atttaccgg 
      agggactgt tag
    mRNA sequence
    • >TC009257 RA
      aggtttcgc ttgcagttg caaggcttc tttttggtc 
      acggatgac atcattgac cggtcggaa atgcgacgt 
      ggaatgccc tgttggtac aagaaggac gacaccggt 
      ttaaatgcc ttcaacgat gccattctt ctcgaacac 
      ggcatttat accttgctg aaaaggcat ttctcgcaa 
      caccattgc tacgttccc accatggag ctcttccat 
      gatgtcacg ctcaaaact tcaatggga caagctcta 
      gattgtttg tgtaataaa gacggaaaa ccgaatttg 
      gaactcttc actatgaat aaatacaac tcgattgtt 
      aaatacaag actgcttat tacacgttc caactaccg 
      gtggctcta gccatgtat atggcgaat ttgtacgac 
      ccagaaatg caccgacag gccaaaacc atcttgatg 
      gaaatgggg cttttcttc caaattcag gacgatttc 
      ctggactgc ttcggcgac cctgaggtg acgggcaag 
      aagggcaac gacatccgc gagggcaag tgctcctgg 
      ctggctgtg gtggccctc caaagggcc aaccccacc 
      cagcgaaaa ataatggag gagtattac ggccgtccc 
      gaccctgaa gcagtccga atcatccga aacctgtac 
      gaagagttg agtttgcca aacacttat gccatctat 
      gaggaggag agcttcaac atcatccgt acacacatc 
      cagcaaatt tccaaagga cttccacac aagttgttt 
      ttcaaaatt atggagaaa atttaccgg agggactgt 
      tagtagtgt gtgtgtgaa tcagtattt tgattattt 
      gcaagataa ttgagttaa ttttaaggt ttattttat 
      tggaattta ttgcttttg atatttttt tatttagct 
      gtgctgaat aagtgtttg attcagttg taattaagc 
      tttaattct attgggtat ttggagtta tcttgtgaa 
      ttttcgagt ctttttctg tgtgatatc gtgcaggct 
      gggttagtc gattagtcg taggccagt cggcattaa 
      gttagtcgt atttgtaag ctgcacaag tttgaaata 
      ttgtcgaat attaacaca cttgtgttt ttccttttt 
      tcctggtaa gttttgtta ccaaccaga aaagtcatt 
      cgattttta taaggtgca tgtctttaa tcattgtta 
      cgaagtaat aataataaa tatacgttt ataat