Status
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Gene information
General information
TC009245 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2569421..2578832
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009245
    iBeetle screen iB_01514

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50
    Only 24 eggs are on the slide.
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009245 RA
      MMVLRVHGD DFVFPNSLH QVSPRIPQM NMMKVHIPF 
      TFKLLETSK STYSEVQCS LSSQVEYQI QAFWGVNIR 
      ELHIFLWRP WSDLREAFQ RGDFLKGFY QYPGVQLKK 
      TPHSDQTIT LKLPAASLN LGTPPRLCY PLVIFLFRT 
      DAEDNIHPD ETVALINVV HIKDSVCTL PTSILAQYL 
      KQANGQLSC LKQLYLATG NPGGYEEGQ IPLAVAEPV 
      EGGPGPLGR TLVCPTSDD SALWNSSGE QLCVVCQYF 
      PLSRALLPC RHTCICASC FVKLDRCPM CRGPIKSYF 
      CIRGEEYMP IQPSSKTVN THSPLYNWL DSWNDRLTD 
      FLGFQR
    • >TC009245 RB
      MTTLLVSAA ELSNIFSVI AVLLCFCAM ILFITRNMM 
      VLRVHGDDF VFPNSLHQV SPRIPQMNM MKVHIPFTF 
      KLLETSKST YSEVQCSLS SQVEYQIQA FWGVNIREL 
      HIFLWRPWS DLREAFQRG DFLKGFYQY PGVQLKKTP 
      HSDQTITLK LPAASLNLG TPPRLCYPL VIFLFRTDA 
      EDNIHPDET VALINVVHI KDSVCTLPT SILAQYLKQ 
      ANGQLSCLK QLYLATGNP GGYEEGQIP LAVAEPVEG 
      GPGPLGRTL VCPTSDDSA LWNSSGEQL CVVCQYFPL 
      SRALLPCRH TCICASCFV KLDRCPMCR GPIKSYFCI 
      RGEEYMPIQ PSSKTVNTH SPLYNWLDS WNDRLTDFL 
      GFQR
    CDS sequence
    • >TC009245 RA
      atgatggtg ctgcgagtt cacggcgac gatttcgtt 
      tttccaaat tccctccat caggtatcg cctaggata 
      cctcaaatg aacatgatg aaagttcac attcccttc 
      actttcaag ctcttagaa acgtccaaa tccacgtat 
      tcagaagtg cagtgcagt ttatctagt caggtcgag 
      taccaaatc caggcgttt tggggcgtg aacatccgc 
      gagttgcac atttttttg tggcgccct tggtccgac 
      ctccgtgag gccttccaa agaggcgat tttctcaaa 
      ggtttttac caatatccc ggcgtccaa ttgaaaaaa 
      actccacat tcggatcaa acaattacg ctgaaatta 
      ccagcggcg tcgctaaat ctggggact ccccctagg 
      ttgtgttac cctttggtc attttcctg tttcggacc 
      gatgctgaa gataacatc catccggac gaaacggtg 
      gcgctgatc aacgtggtg cacattaag gactcggtg 
      tgcactcta cccacaagc atcctcgca cagtatctc 
      aagcaggcc aatggacaa ttgtcctgt ctgaagcaa 
      ttgtacttg gcgacgggc aacccgggg ggctacgag 
      gaggggcag atcccgctg gcggtggcc gagccggtg 
      gaagggggc ccggggccg ctggggcgc acgctcgtg 
      tgtccgacg agcgacgac tcagccctg tggaactcc 
      tccggagag cagttgtgc gtcgtttgt cagtacttt 
      ccgctctcg agggctttg ctcccgtgc aggcacact 
      tgtatttgt gcgagctgt tttgtgaag ctcgatcgg 
      tgtccgatg tgtcggggg cccatcaag agctacttc 
      tgtatcagg ggggaggag tacatgccc attcagccg 
      agctccaag acggttaat acacacagc cccctttac 
      aactggttg gatagttgg aatgatcgc cttacggac 
      tttctcggc tttcagcgg taa
    • >TC009245 RB
      atgactacc cttctagtg tccgcggcc gaattgtcg 
      aacattttt tccgtaatt gcagtgctg ttatgtttt 
      tgtgcgatg atcctgttc atcaccaga aacatgatg 
      gtgctgcga gttcacggc gacgatttc gtttttcca 
      aattccctc catcaggta tcgcctagg atacctcaa 
      atgaacatg atgaaagtt cacattccc ttcactttc 
      aagctctta gaaacgtcc aaatccacg tattcagaa 
      gtgcagtgc agtttatct agtcaggtc gagtaccaa 
      atccaggcg ttttggggc gtgaacatc cgcgagttg 
      cacattttt ttgtggcgc ccttggtcc gacctccgt 
      gaggccttc caaagaggc gattttctc aaaggtttt 
      taccaatat cccggcgtc caattgaaa aaaactcca 
      cattcggat caaacaatt acgctgaaa ttaccagcg 
      gcgtcgcta aatctgggg actccccct aggttgtgt 
      taccctttg gtcattttc ctgtttcgg accgatgct 
      gaagataac atccatccg gacgaaacg gtggcgctg 
      atcaacgtg gtgcacatt aaggactcg gtgtgcact 
      ctacccaca agcatcctc gcacagtat ctcaagcag 
      gccaatgga caattgtcc tgtctgaag caattgtac 
      ttggcgacg ggcaacccg gggggctac gaggagggg 
      cagatcccg ctggcggtg gccgagccg gtggaaggg 
      ggcccgggg ccgctgggg cgcacgctc gtgtgtccg 
      acgagcgac gactcagcc ctgtggaac tcctccgga 
      gagcagttg tgcgtcgtt tgtcagtac tttccgctc 
      tcgagggct ttgctcccg tgcaggcac acttgtatt 
      tgtgcgagc tgttttgtg aagctcgat cggtgtccg 
      atgtgtcgg gggcccatc aagagctac ttctgtatc 
      aggggggag gagtacatg cccattcag ccgagctcc 
      aagacggtt aatacacac agccccctt tacaactgg 
      ttggatagt tggaatgat cgccttacg gactttctc 
      ggctttcag cggtaa
    mRNA sequence
    • >TC009245 RA
      tgttttacc gaccagggt cagctgtgt ttgtgtcaa 
      attgactcc caaatatga attaatgaa ttgattgtt 
      tgtttaaat gttactgtt tattggaat ctcgtcact 
      ggtagcgcc attgcggag agttttctg ctgctgttg 
      gtgatttcc acgcaatgg tgtgcccca taaccccca 
      ccgaaacgt cactttgac gtttctgtc atgccaaat 
      aaaccacta ggtttgaga tcagaaaac agtcaggtt 
      cgggccacc atgtgttga gcgtgttcg aaatgttgc 
      aataaatcg gttgtgtac gaagacatg gtgacaatg 
      actaccctt ctagtgtcc gcggccgaa ttgtcgaac 
      attttttcc gtaattgca gtgctgtta tgtttttgt 
      gcgatgatc ctgttcatc accagaaac atgatggtg 
      ctgcgagtt cacggcgac gatttcgtt tttccaaat 
      tccctccat caggtatcg cctaggata cctcaaatg 
      aacatgatg aaagttcac attcccttc actttcaag 
      ctcttagaa acgtccaaa tccacgtat tcagaagtg 
      cagtgcagt ttatctagt caggtcgag taccaaatc 
      caggcgttt tggggcgtg aacatccgc gagttgcac 
      atttttttg tggcgccct tggtccgac ctccgtgag 
      gccttccaa agaggcgat tttctcaaa ggtttttac 
      caatatccc ggcgtccaa ttgaaaaaa actccacat 
      tcggatcaa acaattacg ctgaaatta ccagcggcg 
      tcgctaaat ctggggact ccccctagg ttgtgttac 
      cctttggtc attttcctg tttcggacc gatgctgaa 
      gataacatc catccggac gaaacggtg gcgctgatc 
      aacgtggtg cacattaag gactcggtg tgcactcta 
      cccacaagc atcctcgca cagtatctc aagcaggcc 
      aatggacaa ttgtcctgt ctgaagcaa ttgtacttg 
      gcgacgggc aacccgggg ggctacgag gaggggcag 
      atcccgctg gcggtggcc gagccggtg gaagggggc 
      ccggggccg ctggggcgc acgctcgtg tgtccgacg 
      agcgacgac tcagccctg tggaactcc tccggagag 
      cagttgtgc gtcgtttgt cagtacttt ccgctctcg 
      agggctttg ctcccgtgc aggcacact tgtatttgt 
      gcgagctgt tttgtgaag ctcgatcgg tgtccgatg 
      tgtcggggg cccatcaag agctacttc tgtatcagg 
      ggggaggag tacatgccc attcagccg agctccaag 
      acggttaat acacacagc cccctttac aactggttg 
      gatagttgg aatgatcgc cttacggac tttctcggc 
      tttcagcgg taacgagtc gagcggtat ttattcaaa 
      ttttcttaa ttattatta gaacaaaat tgtgtagtt 
      tagccacta ttactcaat gtttctcaa gtaaacatc 
      actacagaa aaacgattt tttataagg taggttgtg 
      aggtagcgt tacgtgtaa gatattgaa tatatgtcc 
      atatgactt agtcttaaa tatttatgt ccttttatg 
      caataacat tttttcttt attgtacat tgactttac 
      actatattt tagtatttt catctgact taagcctgt 
      gataatata tacgttaaa aataa
    • >TC009245 RB
      tgttttacc gaccagggt cagctgtgt ttgtgtcaa 
      attgactcc caaatatga attaatgaa ttgattgtt 
      tgtttaaat gttactgtt tattggaat ctcgtcact 
      ggtagcgcc attgcggag agttttctg ctgctgttg 
      gtgatttcc acgcaatgg tgtgcccca taaccccca 
      ccgaaacgt cactttgac gtttctgtc atgccaaat 
      aaaccacta ggtttgaga tcagaaaac agtcaggtt 
      cgggccacc atgtgttga gcgtgttcg aaatgttgc 
      aataaatcg gttgtgtac gaagacatg gtgacaatg 
      actaccctt ctagtgtcc gcggccgaa ttgtcgaac 
      attttttcc gtaattgca gtgctgtta tgtttttgt 
      gcgatgatc ctgttcatc accagaaac atgatggtg 
      ctgcgagtt cacggcgac gatttcgtt tttccaaat 
      tccctccat caggtatcg cctaggata cctcaaatg 
      aacatgatg aaagttcac attcccttc actttcaag 
      ctcttagaa acgtccaaa tccacgtat tcagaagtg 
      cagtgcagt ttatctagt caggtcgag taccaaatc 
      caggcgttt tggggcgtg aacatccgc gagttgcac 
      atttttttg tggcgccct tggtccgac ctccgtgag 
      gccttccaa agaggcgat tttctcaaa ggtttttac 
      caatatccc ggcgtccaa ttgaaaaaa actccacat 
      tcggatcaa acaattacg ctgaaatta ccagcggcg 
      tcgctaaat ctggggact ccccctagg ttgtgttac 
      cctttggtc attttcctg tttcggacc gatgctgaa 
      gataacatc catccggac gaaacggtg gcgctgatc 
      aacgtggtg cacattaag gactcggtg tgcactcta 
      cccacaagc atcctcgca cagtatctc aagcaggcc 
      aatggacaa ttgtcctgt ctgaagcaa ttgtacttg 
      gcgacgggc aacccgggg ggctacgag gaggggcag 
      atcccgctg gcggtggcc gagccggtg gaagggggc 
      ccggggccg ctggggcgc acgctcgtg tgtccgacg 
      agcgacgac tcagccctg tggaactcc tccggagag 
      cagttgtgc gtcgtttgt cagtacttt ccgctctcg 
      agggctttg ctcccgtgc aggcacact tgtatttgt 
      gcgagctgt tttgtgaag ctcgatcgg tgtccgatg 
      tgtcggggg cccatcaag agctacttc tgtatcagg 
      ggggaggag tacatgccc attcagccg agctccaag 
      acggttaat acacacagc cccctttac aactggttg 
      gatagttgg aatgatcgc cttacggac tttctcggc 
      tttcagcgg taacgagtc gagcggtat ttattcaaa 
      ttttcttaa ttattatta gaacaaaat tgtgtagtt 
      tagccacta ttactcaat gtttctcaa gtaaacatc 
      actacagaa aaacgattt tttataagg taggttgtg 
      aggtagcgt tacgtgtaa gatattgaa tatatgtcc 
      atatgactt agtcttaaa tatttatgt ccttttatg 
      caataacat tttttcttt attgtacat tgactttac 
      actatattt tagtatttt catctgact taagcctgt 
      gataatata tacgttaaa aataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY