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Gene information
General information
TC034764 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 845060..853398
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034764
    iBeetle screen iB_01507
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034764 RA
      MFKPRTSFK DIILGLKNV ATTSEGQLK NSIKIDKVL 
      VLSKLSRYE FEKKQSKFS NDDDLEQEL RSRGTDFEW 
      LIHCHMLHK KFETNVVNT FRRMGIDVQ VANRFNYTQ 
      DKVDWADVI VPTGGDGTF LLASSRIRD NTKPVIGFN 
      SDPNRSEGH LCLPKKYSA NIQSAIEKL QNGDFDWLL 
      RSRIRVKLI SQKGDIVPK CLHEIEDNF GKIHGKILP 
      VLALNEVFV GESISSRVS HLQLRLNGS VEQTSIKCS 
      GVCVCTGTG STSWHLSIN RLPAQSVSE LLKLVEVDS 
      REEREVLAA KIADEYNRN LIFRPDDSR MSYTIRDLI 
      SAGVWPHPK GIKSRDFIT KLEIKSNCY KASLVIDGG 
      VSFDFNDGT IAFLEIRPE DSLRTVVLR P
    CDS sequence
    • >TC034764 RA
      atgtttaag ccacggact tcatttaag gatattatt 
      ctagggctc aaaaatgtg gccacaaca tccgaaggc 
      cagctcaag aattctatt aaaattgat aaggtctta 
      gttttatca aaactgtcg cgttatgag ttcgagaaa 
      aagcaatca aagttcagc aacgacgac gatctcgag 
      caagagctt cgaagtcga ggaaccgac ttcgagtgg 
      ctcatacat tgccacatg ctgcacaaa aagttcgaa 
      acgaacgtg gtcaacaca tttaggaga atgggaatc 
      gacgtacaa gtggccaac agattcaat tacacacag 
      gacaaggtg gattgggcg gacgttata gtgccaact 
      gggggcgat ggcacgttt cttttagct tcgagtcgg 
      attagggac aacacgaaa ccggtgatt ggcttcaac 
      tcggacccc aatcgctcc gagggccac ctctgtctc 
      cccaaaaaa tactcggcg aacattcaa agcgccatc 
      gaaaagctg caaaacggg gactttgac tggctgctg 
      cggagccga attcgcgtc aagctcatc agccagaag 
      ggcgatatt gtgccgaaa tgcctccac gagatcgag 
      gacaacttt gggaaaatc cacggcaag attctcccc 
      gttttagct ttaaatgaa gtttttgtg ggagagtca 
      atctcttcc cgcgtctcc cacctgcaa ttgcgcctc 
      aacggctcc gtggagcaa accagcatc aaatgttcc 
      ggggtgtgc gtttgcacc ggaacaggg tcgacttcc 
      tggcacttg agcatcaat cgacttccg gcacaaagc 
      gtgtccgag cttctcaag ctcgttgag gtggattcc 
      agagaagaa cgcgaggtt ttggcggcg aaaatcgcc 
      gacgagtac aacagaaac ctcattttc agacctgat 
      gactcacgg atgagttac acgatccgc gatttaata 
      tcggctggc gtttggccg catcccaag ggaatcaag 
      tccagggat tttatcaca aagttggag atcaagtcg 
      aattgttat aaagcgagc cttgttatc gacggaggc 
      gtttcgttc gattttaat gacggcacg attgcgttt 
      cttgaaatc agaccagag gattcttta cgaactgtc 
      gtgctgcgg ccctaa
    mRNA sequence
    • >TC034764 RA
      aaaaaaata ttttttcac tttcaaatt gtttaattt 
      cgcattttg gacgtcaaa attaacgcc gttatgaat 
      aaacaactt tgttttaat caatctaac gctcgaaaa 
      ataaaataa ttcacgaaa ttttttttt ggtaaaaat 
      cgaaggcgc gtccttttc tcggccccg tcaactcta 
      cctgtctgt cgaataatt ccgctcaat agccctaag 
      ttgttataa gtatccaca ctgcatctc ctaactgaa 
      gtagtgact aacattaga ggatagagc atgattgca 
      cgatttgca caaattttg agactagag aagcaaatt 
      gtggcatga ggcaggctt ttggttgtt tgttgtgga 
      tgcgcgttt ttccttcac aacaaacga aaaactgac 
      ggcatgttt agtgaggaa agcttttca aaaaaatta 
      acactccac ctcacagaa gataggcca ttttacgat 
      atttacgtc atgtacacc ggcgaaaat ttcgcgcga 
      aagaggaca cagtgcccg agtttcgcg caatgtgcc 
      cttggcgcg gaattttgg cccctcatc ccgatgttt 
      taaatgtaa atatatcat tgttgatgt agttttatc 
      atatcattc gaaaataaa tatttaagt aaagttgaa 
      ttttttgtg tgtgtatgc aaccaacgc gacatctac 
      tagtggttc gccaatcgg tcaagccgc aatatgagg 
      tcggtttct cgccgaaaa ttcagcacg tttcgagaa 
      aataaaaat cgcacgttg tgaaatcac tgataagag 
      ggttccaac tttggtgca aagttcaac gcaattagc 
      tcctaacct atactcaaa catacatcc atttatttg 
      gtagtttta catgtgatt tccaataca aaccgaaaa 
      cgatccgat gtttaagcc acggacttc atttaagga 
      tattattct agggctcaa aaatgtggc cacaacatc 
      cgaaggcca gctcaagaa ttctattaa aattgataa 
      ggtcttagt tttatcaaa actgtcgcg ttatgagtt 
      cgagaaaaa gcaatcaaa gttcagcaa cgacgacga 
      tctcgagca agagcttcg aagtcgagg aaccgactt 
      cgagtggct catacattg ccacatgct gcacaaaaa 
      gttcgaaac gaacgtggt caacacatt taggagaat 
      gggaatcga cgtacaagt ggccaacag attcaatta 
      cacacagga caaggtgga ttgggcgga cgttatagt 
      gccaactgg gggcgatgg cacgtttct tttagcttc 
      gagtcggat tagggacaa cacgaaacc ggtgattgg 
      cttcaactc ggaccccaa tcgctccga gggccacct 
      ctgtctccc caaaaaata ctcggcgaa cattcaaag 
      cgccatcga aaagctgca aaacgggga ctttgactg 
      gctgctgcg gagccgaat tcgcgtcaa gctcatcag 
      ccagaaggg cgatattgt gccgaaatg cctccacga 
      gatcgagga caactttgg gaaaatcca cggcaagat 
      tctccccgt tttagcttt aaatgaagt ttttgtggg 
      agagtcaat ctcttcccg cgtctccca cctgcaatt 
      gcgcctcaa cggctccgt ggagcaaac cagcatcaa 
      atgttccgg ggtgtgcgt ttgcaccgg aacagggtc 
      gacttcctg gcacttgag catcaatcg acttccggc 
      acaaagcgt gtccgagct tctcaagct cgttgaggt 
      ggattccag agaagaacg cgaggtttt ggcggcgaa 
      aatcgccga cgagtacaa cagaaacct cattttcag 
      acctgatga ctcacggat gagttacac gatccgcga 
      tttaatatc ggctggcgt ttggccgca tcccaaggg 
      aatcaagtc cagggattt tatcacaaa gttggagat 
      caagtcgaa ttgttataa agcgagcct tgttatcga 
      cggaggcgt ttcgttcga ttttaatga cggcacgat 
      tgcgtttct tgaaatcag accagagga ttctttacg 
      aactgtcgt gctgcggcc ctaatttta agcatcact 
      gtttttttt gtatgtaat aataataaa gtaaagtac 
      cacca