Status
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Gene information
General information
TC033576
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009225,TC009224
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 870278..874647
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033576
    iBeetle screen iB_01505
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    ovary size decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4

    previtellogenic egg chamber mostlynot present - Number of animals showing the phenotype: 4
    mature egg completenot present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 3
    antenna orientation irregular - Number of animals showing the phenotype: 3

    wings shortened - Number of animals showing the phenotype: 3

    iBeetle screen iB_01506
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033576 RA
      MYEISEVED NEGSRPILV EFQNSKLQE GQENHLKTA 
      IFRENRDTI VAAASKKLL YTGRVSGDQ DQDLHKRFL 
      LISNRRTGK VRLVAVDSV ILSPQFPEK SRGSISEKN 
      VNSVNKLHK AFGSKKIKR ITEQRERMK MNIDDVREQ 
      LEQTVAGIE IDDSGPLIV ERAENDSAY RPPIHRDAE 
      SREEVYILN EIVPLKILN SLEKETREI FDSDLDSTE 
      LAPMVREKF AQMQNSSSP DVQKCELLL YLDYLIKFI 
      SKPARSITK KFVICPTSQ QVNVHILDH FTVSSNTGR 
      TRPVSMRDK TVCYILVIA MIAFDYEVD LEQLAKVIK 
      IGVKKLQEM GRILAFSPH PKNKNAMTL KIPLPAAVT 
      SSSGMRIPA TMGPLWWPA TRLEVLGVV GARGAQAEA 
      LRVKPGAHW HVRVASSHS CESGHCEGR AVGSHDSPE 
      KGVLGAKSG KIAACLPKA ELKSRMKKR HLGIVSDLL 
      PQDCFTQGL DYFSEDVSK IGLPKCDKF SRPGICAKY 
      FQTRYDKMV YRPSECAKD GKFGAKQCK GEYTTGRCF 
      CFDAQGERL FGQQWQNQA ENMTCACSR KKADMGDSV 
      YFNSLHCDA MGNYEPLQC DVISGQCWC ADAQTGDLT 
      SPVVPLKAI SKLPCYDSA SVGSQYLRQ CESKLYAEK 
      RILYELKLH GTIASFVDF LCDDDGSYG AYTYVEGAV 
      YCTWRDNSK IGSWASDGN DEVDCNCAR DTKIFEEAG 
      KSQSLKCEP NGNYKKLQK TGSKGYCVD IDGYRKSPS 
      GDDVTDEAL CDGYYAYH
    CDS sequence
    • >TC033576 RA
      atgtacgaa atatccgag gttgaggac aacgagggc 
      tcacgcccg attctcgtg gaatttcaa aatagcaaa 
      cttcaggaa gggcaagaa aatcactta aaaaccgcg 
      atttttagg gaaaatcgt gacacaatc gtcgcggcg 
      gctagtaaa aagcttttg tacacggga cgggtcagt 
      ggtgaccag gaccaggac ctccacaag cggtttttg 
      ctgatttcg aatcggagg actggaaaa gtgcgtcta 
      gtggcagtg gacagcgtg attctctca ccgcaattc 
      cccgaaaag agtcggggg agcataagt gagaaaaat 
      gtaaattcg gtgaataag ttgcacaaa gcgtttggg 
      tccaaaaaa atcaaaaga atcaccgaa cagagggaa 
      aggatgaaa atgaacatt gacgacgtt cgagagcaa 
      ttagaacag acagttgcc ggaattgaa attgatgac 
      agtgggcct ttgattgtc gaaagggcc gaaaatgac 
      agtgcttac aggccccca attcaccga gacgccgaa 
      agtcgggaa gaagtatac attttgaac gaaatcgtg 
      cctttgaaa attttaaat tctttggaa aaagaaact 
      agagaaatt tttgactcg gacttggat tctacagag 
      ttggctcca atggttcgg gaaaagttc gcacaaatg 
      caaaactcg tcctctcca gacgtacaa aagtgcgaa 
      ctactttta tacctcgac tatttaata aaattcatc 
      tcaaaaccg gccagaagt ataacgaaa aaattcgta 
      atttgcccg acttcacaa caagtaaat gtccacatt 
      ttggaccac tttacggtc tcttcaaat acgggaaga 
      acccgtcca gtatcaatg agggacaaa acagtctgt 
      tacattctc gttatcgca atgattgcg tttgactac 
      gaagttgat ttagaacaa ctggccaaa gttataaaa 
      atcggcgtg aaaaaactg caagaaatg ggccgtatt 
      ctagcgttc agtccgcac ccaaaaaac aagaacgcc 
      atgactctg aaaattccg ctcccagcc gccgtgacc 
      tcatcatct ggaatgaga attccggcg acgatgggg 
      ccgctgtgg tggcccgca acaaggctt gaggtgttg 
      ggggtcgtg ggggccagg ggggcgcag gccgaggcg 
      ttgcgcgtg aagccgggg gcgcactgg cacgtgcgc 
      gtggcctcg tcgcacagt tgcgagtcg gggcattgc 
      gaaggcagg gcggtgggg tcgcacgat tcgcctgaa 
      aagggggtg ttgggggca aagtccgga aaaattgct 
      gcttgttta cctaaagcc gagttgaag agcaggatg 
      aagaagagg catctcgga atcgtgtcc gatttgcta 
      ccgcaagac tgtttcacc caaggcctt gactatttt 
      agtgaggat gtctcaaaa attggttta cccaagtgt 
      gataaattt tcaagacca ggtatttgt gctaaatat 
      tttcaaaca aggtacgac aaaatggtc tatagaccg 
      agcgaatgt gccaaagat ggcaaattt ggggcgaaa 
      caatgcaag ggggaatac acaactgga cgatgtttt 
      tgctttgat gctcaaggg gagaggctt ttcggccaa 
      cagtggcaa aatcaggcc gaaaatatg acgtgcgct 
      tgtagccgc aaaaaagca gacatgggc gattccgta 
      tatttcaat agtctacac tgtgacgca atgggcaat 
      tatgagccc ctgcagtgt gacgtaatc agtgggcaa 
      tgttggtgt gcggacgcg caaaccggg gatttaacc 
      tcgccggtg gttcccctg aaagcaatt tcgaaattg 
      ccgtgctac gattcggct tcggtgggc agccaatac 
      ctccgccag tgcgaaagc aaactctat gccgaaaaa 
      cgcatcctt tacgagcta aaattgcac ggaactatt 
      gcaagtttt gttgatttt ttgtgcgat gatgacggc 
      agttacggg gcttatact tacgtggag ggggcggtt 
      tattgcact tggagggat aatagtaaa attgggtcg 
      tgggcttct gatggcaac gacgaagtt gattgcaat 
      tgtgcaaga gacacgaaa attttcgaa gaagcgggg 
      aaatctcag tcgttgaag tgtgagcct aatggtaat 
      tataaaaaa ttgcaaaag acgggctca aagggatat 
      tgtgtggac attgatggg tacaggaag tcgccgtct 
      ggtgacgat gtgacagac gaggcgctc tgtgatggt 
      tattatgcc taccattaa
    mRNA sequence
    • >TC033576 RA
      agggacaag ttgacattt ttttgcacc gcctttaag 
      gatttcatg tagttcccc cagttgtgg ttttttagt 
      tgaatcgtt tcgcttaaa cacttcaaa attacgttg 
      tttttgcat attttgaca ctaatcacg ccttcgttt 
      gtcattttt ctcgaataa aaaaatcga acttgcgcg 
      ctttcggtc gtgggaacg tggtgggaa cacgatgag 
      gttatgtta aacagcgac acgattttt tttaatttt 
      tgatttatt aattttaaa atgtacgaa atatccgag 
      gttgaggac aacgagggc tcacgcccg attctcgtg 
      gaatttcaa aatagcaaa cttcaggaa gggcaagaa 
      aatcactta aaaaccgcg atttttagg gaaaatcgt 
      gacacaatc gtcgcggcg gctagtaaa aagcttttg 
      tacacggga cgggtcagt ggtgaccag gaccaggac 
      ctccacaag cggtttttg ctgatttcg aatcggagg 
      actggaaaa gtgcgtcta gtggcagtg gacagcgtg 
      attctctca ccgcaattc cccgaaaag agtcggggg 
      agcataagt gagaaaaat gtaaattcg gtgaataag 
      ttgcacaaa gcgtttggg tccaaaaaa atcaaaaga 
      atcaccgaa cagagggaa aggatgaaa atgaacatt 
      gacgacgtt cgagagcaa ttagaacag acagttgcc 
      ggaattgaa attgatgac agtgggcct ttgattgtc 
      gaaagggcc gaaaatgac agtgcttac aggccccca 
      attcaccga gacgccgaa agtcgggaa gaagtatac 
      attttgaac gaaatcgtg cctttgaaa attttaaat 
      tctttggaa aaagaaact agagaaatt tttgactcg 
      gacttggat tctacagag ttggctcca atggttcgg 
      gaaaagttc gcacaaatg caaaactcg tcctctcca 
      gacgtacaa aagtgcgaa ctactttta tacctcgac 
      tatttaata aaattcatc tcaaaaccg gccagaagt 
      ataacgaaa aaattcgta atttgcccg acttcacaa 
      caagtaaat gtccacatt ttggaccac tttacggtc 
      tcttcaaat acgggaaga acccgtcca gtatcaatg 
      agggacaaa acagtctgt tacattctc gttatcgca 
      atgattgcg tttgactac gaagttgat ttagaacaa 
      ctggccaaa gttataaaa atcggcgtg aaaaaactg 
      caagaaatg ggccgtatt ctagcgttc agtccgcac 
      ccaaaaaac aagaacgcc atgactctg aaaattccg 
      ctcccagcc gccgtgacc tcatcatct ggaatgaga 
      attccggcg acgatgggg ccgctgtgg tggcccgca 
      acaaggctt gaggtgttg ggggtcgtg ggggccagg 
      ggggcgcag gccgaggcg ttgcgcgtg aagccgggg 
      gcgcactgg cacgtgcgc gtggcctcg tcgcacagt 
      tgcgagtcg gggcattgc gaaggcagg gcggtgggg 
      tcgcacgat tcgcctgaa aagggggtg ttgggggca 
      aagtccgga aaaattgct gcttgttta cctaaagcc 
      gagttgaag agcaggatg aagaagagg catctcgga 
      atcgtgtcc gatttgcta ccgcaagac tgtttcacc 
      caaggcctt gactatttt agtgaggat gtctcaaaa 
      attggttta cccaagtgt gataaattt tcaagacca 
      ggtatttgt gctaaatat tttcaaaca aggtacgac 
      aaaatggtc tatagaccg agcgaatgt gccaaagat 
      ggcaaattt ggggcgaaa caatgcaag ggggaatac 
      acaactgga cgatgtttt tgctttgat gctcaaggg 
      gagaggctt ttcggccaa cagtggcaa aatcaggcc 
      gaaaatatg acgtgcgct tgtagccgc aaaaaagca 
      gacatgggc gattccgta tatttcaat agtctacac 
      tgtgacgca atgggcaat tatgagccc ctgcagtgt 
      gacgtaatc agtgggcaa tgttggtgt gcggacgcg 
      caaaccggg gatttaacc tcgccggtg gttcccctg 
      aaagcaatt tcgaaattg ccgtgctac gattcggct 
      tcggtgggc agccaatac ctccgccag tgcgaaagc 
      aaactctat gccgaaaaa cgcatcctt tacgagcta 
      aaattgcac ggaactatt gcaagtttt gttgatttt 
      ttgtgcgat gatgacggc agttacggg gcttatact 
      tacgtggag ggggcggtt tattgcact tggagggat 
      aatagtaaa attgggtcg tgggcttct gatggcaac 
      gacgaagtt gattgcaat tgtgcaaga gacacgaaa 
      attttcgaa gaagcgggg aaatctcag tcgttgaag 
      tgtgagcct aatggtaat tataaaaaa ttgcaaaag 
      acgggctca aagggatat tgtgtggac attgatggg 
      tacaggaag tcgccgtct ggtgacgat gtgacagac 
      gaggcgctc tgtgatggt tattatgcc taccattaa 
      taaaaacct tcaacaatt tgccaaaac tgttttatt 
      tgcctcttt tcaaactta ataatttat gattatttt 
      t