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Gene information
General information
TC009220 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3028508..3030357
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009220
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01504

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009220 RA
      MDSPQMYDN HQQGQTSNE VKKSVIMNS NNNNNNLTN 
      LNNNTGISK QTTALLKQH HLQQYQQHG ELNDLNTPE 
      ISLDLQNLI DDSQFSDGL FSDILHQSQ GKHLQGLQP 
      RPMAPTNNY PRTTLAYMP QPVHSSASY SATPNSNSD 
      SNSSAGSDV PSIKEEPVD PQEYRRQCN VMPNGYMGG 
      GYPAAPAAT FTTLAPSPV MHHMNAMKS KTMMLNQHI 
      ARKSVKPCD KNSDEYRRR RERNNIAVR KSREKAKVR 
      TRETEEKVK ILMKENERL QKRIELLTE ELNVLRSLF 
      SNVGVLPEH VHREINKHL DTFQLQQQQ QMSEDSRRL 
      METLHEYGP
    CDS sequence
    • >TC009220 RA
      atggattct ccccaaatg tacgacaat catcagcag 
      ggccaaact agcaacgaa gtcaaaaag tcggtcata 
      atgaacagc aataataac aataataat ttaacgaac 
      ttgaataac aatacgggc atttcgaag cagacgaca 
      gcgttgctt aagcagcac cacctccag cagtaccag 
      caacacggc gaactcaac gatctaaac acgccggag 
      atttcctta gacctgcag aacctcatc gacgattcg 
      caattcagc gatgggctt ttctccgac atcctccac 
      cagagccaa ggcaagcac ctgcagggg ctgcagccg 
      cggccgatg gcccccacg aacaactac ccccgcacc 
      acgctggcc tacatgccg cagccggtg cacagcagc 
      gcctcgtac tctgccacc cccaactcc aacagcgac 
      tccaacagt tcagctggg agcgacgtg cccagcatc 
      aaggaggag ccggtggac ccgcaggag taccgccgc 
      cagtgcaac gtcatgccc aacgggtac atggggggc 
      gggtacccc gccgccccc gccgccacc ttcacgacg 
      ctggccccc agccccgtc atgcaccac atgaacgcg 
      atgaagagc aaaacgatg atgctcaac cagcacatc 
      gcacgcaag tctgtcaag ccctgtgat aagaacagt 
      gacgagtac cgccgccgc cgggagcgg aacaacatc 
      gcggtgagg aagagccgg gagaaggcc aaggtgcgg 
      acgcgggag acggaggag aaagtcaag attctgatg 
      aaggaaaac gaaagactg caaaagagg atagagctg 
      ctcacggag gagttgaac gtgctgagg agtctgttc 
      tcgaacgtg ggggtgctg cccgagcac gtgcaccgg 
      gagatcaac aagcatcta gacaccttc cagttgcag 
      cagcagcag caaatgtcc gaagatagt agaaggttg 
      atggagacg ttgcacgaa tacggaccg tag
    mRNA sequence
    • >TC009220 RA
      tggcttggg caagcgcac gaacggtgt ggtccccaa 
      gtaggctgt gtaagagta cacctgaca gacatcagg 
      atcacgttg gcgcgcaaa tcaacgcac cccaggatt 
      cgaattttt tttttgaaa aaaaggcag tgccagtgt 
      ttataatgg atcctgaat ggaatagcg ttcgattca 
      aagcgaatt aaaatttgg gaaagtgcg tagtgcgtg 
      tgttggact tgattgaac tcggtttcg gcagtgaaa 
      gtggactta gtgaaagtc ggtgccgaa aatggattc 
      tccccaaat gtacgacaa tcatcagca gggccaaac 
      tagcaacga agtcaaaaa gtcggtcat aatgaacag 
      caataataa caataataa tttaacgaa cttgaataa 
      caatacggg catttcgaa gcagacgac agcgttgct 
      taagcagca ccacctcca gcagtacca gcaacacgg 
      cgaactcaa cgatctaaa cacgccgga gatttcctt 
      agacctgca gaacctcat cgacgattc gcaattcag 
      cgatgggct tttctccga catcctcca ccagagcca 
      aggcaagca cctgcaggg gctgcagcc gcggccgat 
      ggcccccac gaacaacta cccccgcac cacgctggc 
      ctacatgcc gcagccggt gcacagcag cgcctcgta 
      ctctgccac ccccaactc caacagcga ctccaacag 
      ttcagctgg gagcgacgt gcccagcat caaggagga 
      gccggtgga cccgcagga gtaccgccg ccagtgcaa 
      cgtcatgcc caacgggta catgggggg cgggtaccc 
      cgccgcccc cgccgccac cttcacgac gctggcccc 
      cagccccgt catgcacca catgaacgc gatgaagag 
      caaaacgat gatgctcaa ccagcacat cgcacgcaa 
      gtctgtcaa gccctgtga taagaacag tgacgagta 
      ccgccgccg ccgggagcg gaacaacat cgcggtgag 
      gaagagccg ggagaaggc caaggtgcg gacgcggga 
      gacggagga gaaagtcaa gattctgat gaaggaaaa 
      cgaaagact gcaaaagag gatagagct gctcacgga 
      ggagttgaa cgtgctgag gagtctgtt ctcgaacgt 
      gggggtgct gcccgagca cgtgcaccg ggagatcaa 
      caagcatct agacacctt ccagttgca gcagcagca 
      gcaaatgtc cgaagatag tagaaggtt gatggagac 
      gttgcacga atacggacc gtagccccc acgattaat 
      gacaatcat tcgaattta cggaaagtt tgttccctt 
      gttgaatct tgttcgagg ttcggtagt tagatctcc 
      tatgacaca cgtgttcta tacaaaaac tttttatta 
      ttaatttat tagagtgga gtgtcgtct tgcaaaaga 
      tttccgatt tgtttcaac acaacgaac gaacttttt 
      ctacgcgaa acagcgacg aaatctttg cggcgacca 
      agtttgtta acgggtatt aaaaataca ttagattta 
      ttccgttcg tttttgtga tcaaacaat tgtatttaa 
      aatgcgatg agttatatt tttaaaaac gatgcaatc 
      cgtgtacct tcttaactg ttagtaaat taaatgtat 
      tttaaggta tatttttgt acagaaaat atatcgttg 
      ttacacctt ataatttta ttgtaaaat acttaagtt 
      aatgtataa atgtataat gtatagcgt gtacttgta 
      aatcttaat atatttcca aagaacta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY