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Gene information
General information
TC034768 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 950614..952931
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034768
    iBeetle screen iB_01503
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034768 RA
      MEILEFKDL KCVPDIVHP YTPSLRNNS TPIIIDNGS 
      YQCRAGWAT SSEPLLIFK NLIAKPRKE RSKKDTVEA 
      SQAPQLQVG NDIINIEAV RFQLKTQFD RNVVTHFEA 
      QEHLFDYTF AHLGIDSEN SVPHPIVLT EAVLNPNSS 
      RQLMSELLF ECYSVPGVS YGIDCLYSY HFNEVKPQE 
      NALIINLGY HTCHVVPII GKKMVFENT RRLNTGGFH 
      IISFLHRIL QLKYPAHAT SITLSRAEE LLHSICAIA 
      LDYRKELNR WTDPLYYEE NIKRIQLPY NASLVSSAL 
      TLEQQKERK RELARRLTE INARKREER LIEDEEKLN 
      QLLYIQEMT EAGVDKEEI DRACADYQI KNVQDLQKH 
      INNLNQKIE KTKQKIIAA NTTDEVEEP VAKQAKHTK 
      MVFENEKEM HLFVQNAKK MRQEILNKK MTRKQRKQD 
      MAKRRTAAG QERMRIISQ LARKEKGND DFGLRDEDW 
      DIYKAISKD GGDSDSEAE NEKLLELDD IIRTYEPSE 
      LDETLNPGE THQLHVGIE RYRAPELIF KPYMLGSSE 
      AGLSEVIGY VLSLFNNED QLKLAENVV IMGGLANLP 
      GLQERILSD LISIRPFQS IVNVRTVKN PSLGAWHGA 
      KKWTKSGEF KGSFLTKKM YEEFGSEYF KTHVASNPF 
      YHTPKGQNV DIEMV
    CDS sequence
    • >TC034768 RA
      atggaaatt ctcgaattc aaagacttg aaatgtgtc 
      cccgacatt gtccaccct tacacacca tccctgcgc 
      aacaacagt acccccata atcatcgat aacggttcc 
      taccaatgc agggctggt tgggccacc agtagcgaa 
      cccctgctt atttttaag aatcttatc gcaaaacca 
      cgcaaagaa cgctcaaaa aaagacact gtggaggca 
      agtcaagcc ccccagttg caagttggt aacgatata 
      ataaatatc gaagcggtc cgtttccaa ttaaaaacg 
      caatttgat cgaaatgtg gtcacacat tttgaggca 
      caggagcac ctttttgat tatactttc gcccatttg 
      gggattgat tcggaaaat tcggtgccc catcctatc 
      gttttgact gaggcggtt ttaaacccg aattcttcg 
      agacagtta atgtcagaa ttgctattt gagtgctat 
      tcagtgcct ggggtttct tacgggatt gattgtttg 
      tattcttat cattttaac gaagttaaa ccacaggag 
      aatgcgtta attattaat ttggggtac cacacatgt 
      catgtggtt ccaatcatt ggcaagaag atggttttt 
      gagaatacg agacgttta aatactgga ggctttcac 
      attatttcg tttttgcac agaattttg caattgaaa 
      tatccagcg cacgctacg tctattact ttaagtagg 
      gccgaggaa ttgttgcac agtatttgc gccatagct 
      ttggattat cgcaaagaa cttaatcgt tggacggat 
      ccgttatat tacgaagaa aatatcaaa cggatccag 
      ttaccatac aatgcttca cttgtttcg tcagctctc 
      actttggag caacaaaaa gaacgcaaa agggaattg 
      gctcgacgt cttaccgaa ataaatgcc cgaaaacgt 
      gaagaacgt ctcattgaa gacgaagaa aaactaaac 
      cagctttta tacattcaa gaaatgaca gaagccggg 
      gtcgataaa gaagaaatc gatcgagcc tgtgccgat 
      taccagata aaaaacgtc caggatttg caaaaacac 
      atcaataat ttgaaccaa aagattgag aaaactaaa 
      caaaaaatc atcgcggct aacacgact gacgaagtt 
      gaggaaccg gtcgccaaa caggcgaaa cacacgaaa 
      atggtgttc gagaacgag aaagaaatg catttgttt 
      gtacaaaat gccaaaaaa atgcgccaa gagattttg 
      aataagaag atgactaga aagcaaagg aagcaagac 
      atggccaaa aggcggacg gcagcaggc caggaacga 
      atgcgtatt atatcgcag ttggccaga aaggagaaa 
      gggaacgat gattttggt ttaagggac gaggattgg 
      gacatttac aaggcgatt agtaaagat ggcggagac 
      tcggatagt gaggccgaa aacgagaaa cttttagaa 
      cttgatgac ataattcgg acttatgaa ccttcggaa 
      cttgacgaa acgctcaat ccgggggaa acacaccaa 
      ctacatgtg gggatcgaa cgttacaga gccccagaa 
      ttgattttt aaaccttat atgttgggt tcgtcagaa 
      gccggtttg tcggaagtc attggttac gttctgtcg 
      ctgtttaac aacgaggat cagttaaaa ctggccgag 
      aatgttgtt ataatgggc ggtttggcc aatttgcct 
      ggtctacaa gaacggatt ctttcagat ttgatcagt 
      attcgacca tttcagtcc attgttaac gtaaggacg 
      gtcaaaaac ccgagcttg ggggcctgg catggggcg 
      aaaaagtgg acaaaatcg ggggagttt aaaggcagt 
      tttctgacg aagaaaatg tacgaggag tttggatct 
      gagtatttt aagacacat gtggcatca aatccattt 
      tatcacacg ccaaaaggg caaaatgtc gatattgag 
      atggtctaa
    mRNA sequence
    • >TC034768 RA
      cgtcactta acccacaaa aaatggaaa ttctcgaat 
      tcaaagact tgaaatgtg tccccgaca ttgtccacc 
      cttacacac catccctgc gcaacaaca gtaccccca 
      taatcatcg ataacggtt cctaccaat gcagggctg 
      gttgggcca ccagtagcg aacccctgc ttattttta 
      agaatctta tcgcaaaac cacgcaaag aacgctcaa 
      aaaaagaca ctgtggagg caagtcaag ccccccagt 
      tgcaagttg gtaacgata taataaata tcgaagcgg 
      tccgtttcc aattaaaaa cgcaatttg atcgaaatg 
      tggtcacac attttgagg cacaggagc acctttttg 
      attatactt tcgcccatt tggggattg attcggaaa 
      attcggtgc cccatccta tcgttttga ctgaggcgg 
      ttttaaacc cgaattctt cgagacagt taatgtcag 
      aattgctat ttgagtgct attcagtgc ctggggttt 
      cttacggga ttgattgtt tgtattctt atcatttta 
      acgaagtta aaccacagg agaatgcgt taattatta 
      atttggggt accacacat gtcatgtgg ttccaatca 
      ttggcaaga agatggttt ttgagaata cgagacgtt 
      taaatactg gaggctttc acattattt cgtttttgc 
      acagaattt tgcaattga aatatccag cgcacgcta 
      cgtctatta ctttaagta gggccgagg aattgttgc 
      acagtattt gcgccatag ctttggatt atcgcaaag 
      aacttaatc gttggacgg atccgttat attacgaag 
      aaaatatca aacggatcc agttaccat acaatgctt 
      cacttgttt cgtcagctc tcactttgg agcaacaaa 
      aagaacgca aaagggaat tggctcgac gtcttaccg 
      aaataaatg cccgaaaac gtgaagaac gtctcattg 
      aagacgaag aaaaactaa accagcttt tatacattc 
      aagaaatga cagaagccg gggtcgata aagaagaaa 
      tcgatcgag cctgtgccg attaccaga taaaaaacg 
      tccaggatt tgcaaaaac acatcaata atttgaacc 
      aaaagattg agaaaacta aacaaaaaa tcatcgcgg 
      ctaacacga ctgacgaag ttgaggaac cggtcgcca 
      aacaggcga aacacacga aaatggtgt tcgagaacg 
      agaaagaaa tgcatttgt ttgtacaaa atgccaaaa 
      aaatgcgcc aagagattt tgaataaga agatgacta 
      gaaagcaaa ggaagcaag acatggcca aaaggcgga 
      cggcagcag gccaggaac gaatgcgta ttatatcgc 
      agttggcca gaaaggaga aagggaacg atgattttg 
      gtttaaggg acgaggatt gggacattt acaaggcga 
      ttagtaaag atggcggag actcggata gtgaggccg 
      aaaacgaga aacttttag aacttgatg acataattc 
      ggacttatg aaccttcgg aacttgacg aaacgctca 
      atccggggg aaacacacc aactacatg tggggatcg 
      aacgttaca gagccccag aattgattt ttaaacctt 
      atatgttgg gttcgtcag aagccggtt tgtcggaag 
      tcattggtt acgttctgt cgctgttta acaacgagg 
      atcagttaa aactggccg agaatgttg ttataatgg 
      gcggtttgg ccaatttgc ctggtctac aagaacgga 
      ttctttcag atttgatca gtattcgac catttcagt 
      ccattgtta acgtaagga cggtcaaaa acccgagct 
      tgggggcct ggcatgggg cgaaaaagt ggacaaaat 
      cgggggagt ttaaaggca gttttctga cgaagaaaa 
      tgtacgagg agtttggat ctgagtatt ttaagacac 
      atgtggcat caaatccat tttatcaca cgccaaaag 
      ggcaaaatg tcgatattg agatggtct aataaagtg 
      tgttgtaat gtcactgtt gtttttttt ttttattaa 
      ttcccctaa actcatttg agtaat