Status
Please wait ...
Gene information
General information
TC033581
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009216,TC009217
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 961467..971715
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033581
    iBeetle screen iB_01502
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033581 RA
      MVKLLKQSV LFWALVLSV KTEDIRRNN SKNNNPGFF 
      EAENVRKLE TDENFDEQL KLGSRFSPE NEENLQRKW 
      GRKIGLDEN LLSRDGLNK RDGENGGKN EERFDDRHI 
      KMHRPKNAN QERNTEDRR SKIEESDAR HKFMNKQSE 
      KLQRGTEER IDDRQPHRC GQKLHRPEN VNRGKIDKE 
      NIRSRFLNE ASRLEEHFR DNNPRSRRF GLNTELNKR 
      FNEINSRRT GLNDRLARN DPHKFKFPT DQAEKLGRK 
      NHNRIEKRQ KGRMQRPEE ANLERSTDD RRSKIEESD 
      ARHRFNPEF LNEQHEKLQ RRTEERNDA RQFHRFGQK 
      LRRPENVNL GRQEKLEKS GFKTSVGCD KFQISDIKH 
      RGGDDAPIM ASFSWLCPL VALTLLTLT TAQFSDYSD 
      LRGPYCQRR GCCPGRQDD CSEPILGNN EVISEGTLC 
      YCDDFCNRT RNEDCCPDF WTHCKGLPP PPEPPSPIL 
      GCTYEGRVY PLNKQIKKN CNVCKCEKM GQNQADMLC 
      EQHQCLIEP SITEAINSN YANYGWSAS NYSKFWGHK 
      LEEGIKLRL GTLQPQRFV MHMNPVRRI YDPNSLPRE 
      FDSEFKWPG WMSEIQDQG WCGSSWAIT TAAVASDRF 
      AILSKGREK VTLSAQHLL SCDRRGQQS CNGGYLDRA 
      WSYIRKIGL VDEQCFPYS ATNEKCRIP RRGDLVTAN 
      CQLPTNVDR RSKYKVAPA YRVGNETDI MYEILHSGP 
      VQATMKVYH DFFTYKRGI YRHSPISTN DRTGYHSVR 
      IVGWGEEYS PEGLKKYWK VANSWGPEW GENGYFRIL 
      RGSNECEIE SFVLGTWAE VENKLLLRN EI
    • >TC033581 RB
      MVKLLKQSV LFWALVLSV KTEDIRRNN SKNNNPGFF 
      EAENVRKLE TDENFDEQL KLGSRFSPE NEENLQRKW 
      GRKIGLDEN LLSRDGLNK RDGENGGKN EERFDDRHI 
      KMHRPKNAN QERNTEDRR SKIEESDAR HKFMNKQSE 
      KLQRGTEER IDDRQPHRC GQKLHRPEN VNRGKIDKE 
      NIRSRFLNE ASRLEEHFR DNNPRSRRF GLNTELNKR 
      FNEINSRRT GLNDRLARN DPHKFKFPT DQAEKLGRK 
      NHNRIEKRQ KGRMQRPEE ANLERSTDD RRSKIEESD 
      ARHRFNPEF LNEQHEKLQ RRTEERNDA RQFHRFGQK 
      LRRPENVNL GRQEKLEKS GFKTSVGCD KFQISDIKH 
      RGGDDAPIM ASFSWLCPL VALTLLTLT TAQFSDYSD 
      LRGPYCQRR GCCPGRQDD CSEPILGTL CYCDDFCNR 
      TRNEDCCPD FWTHCKGLP PPPEPPSPI LGCTYEGRV 
      YPLNKQIKK NCNVCKCEK MGQNQADML CEQHQCLIE 
      PSITEAINS NYANYGWSA SNYSKFWGH KLEEGIKLR 
      LGTLQPQRF VMHMNPVRR IYDPNSLPR EFDSEFKWP 
      GWMSEIQDQ GWCGSSWAI TTAAVASDR FAILSKGRE 
      KVTLSAQHL LSCDRRGQQ SCNGGYLDR AWSYIRKIG 
      LVDEQCFPY SATNEKCRI PRRGDLVTA NCQLPTNVD 
      RRSKYKVAP AYRVGNETD IMYEILHSG PVQATMKVY 
      HDFFTYKRG IYRHSPIST NDRTGYHSV RIVGWGEEY 
      SPEGLKKYW KVANSWGPE WGENGYFRI LRGSNECEI 
      ESFVLGTWA EVENKLLLR NEI
    CDS sequence
    • >TC033581 RA
      atggttaag ttattaaag caatctgtg ctattttgg 
      gccttggtc ctcagcgtc aaaactgaa gatataaga 
      cgtaacaat tcaaaaaat aacaatcca ggttttttc 
      gaagcggag aacgtaaga aaactagaa actgatgaa 
      aattttgat gaacaatta aagttgggt tcacggttt 
      tcaccagag aatgaagaa aatcttcaa aggaaatgg 
      ggtcgtaaa attggttta gacgaaaac ttattgtct 
      cgtgatggt ctgaataaa cgtgacgga gaaaacgga 
      ggaaaaaat gaagaaaga tttgatgac aggcacata 
      aaaatgcat cgtccaaaa aatgcaaat caagagaga 
      aatacagaa gacagaaga agtaaaatt gaggaaagt 
      gatgctcgc cacaaattt atgaataaa caaagtgaa 
      aaattacaa agagggact gaagaaaga atcgacgac 
      agacaacca cacagatgt ggtcaaaaa ttgcatcgc 
      ccagaaaat gtaaatcga ggaaaaatt gataaagaa 
      aacataaga agccgtttc ttaaatgaa gcatcaaga 
      ttagaagaa catttcagg gacaacaat cctagatcc 
      agaagattt ggtctgaac acagaactt aacaaaaga 
      tttaatgaa attaattca cgtagaact ggtttaaac 
      gacagatta gctcgtaat gatcctcac aaattcaag 
      tttccaaca gatcaagcc gaaaaatta ggaagaaaa 
      aatcacaac agaattgaa aaaagacaa aaaggaaga 
      atgcagcgt ccagaagaa gctaatcta gagagaagt 
      acagacgac agaagaagt aaaattgaa gaaagtgat 
      gctcgccat agatttaat ccagaattt ctgaacgaa 
      caacacgaa aaattacaa agacggact gaggaaaga 
      aatgacgcc agacaattt cacagattc ggtcaaaaa 
      ttgcgacgt ccagagaat gtaaatctc ggaagacag 
      gagaagttg gaaaaatct ggattcaaa acgagtgtt 
      ggctgtgac aagtttcag atcagtgat atcaagcat 
      cgaggcggc gacgacgct ccgattatg gcctccttc 
      agctggctt tgccccctt gtggccctc accctcctc 
      accctaacc accgcccaa ttcagcgac tactctgat 
      ctccggggc ccttactgc caacggcgg ggctgttgc 
      cccggccgc caagacgat tgttccgag cctatttta 
      ggcaataac gaagtcatt tccgaaggt acattgtgc 
      tactgtgac gatttctgc aaccggaca cgaaacgaa 
      gattgttgt cccgacttt tggacgcat tgcaagggc 
      cttccgccc ccgcccgag cccccatcc cccattcta 
      gggtgcacg tacgaaggg agagtgtac ccccttaac 
      aaacaaatt aagaagaat tgcaatgtc tgtaagtgt 
      gaaaaaatg ggccagaat caggccgat atgttgtgc 
      gagcagcac cagtgtttg attgaacca tcgattacg 
      gaagcaatt aactctaat tatgccaat tatggatgg 
      agtgcttcg aattattcg aaattctgg ggccataag 
      ctggaagaa gggattaaa ttaaggtta gggactctc 
      caaccgcag cgttttgtc atgcacatg aacccggtg 
      aggaggatt tacgaccca aattctctc ccaagagaa 
      ttcgattcg gaatttaag tggcctgga tggatgtca 
      gaaatacag gatcaaggg tggtgtggc tcctcctgg 
      gccataacc acggctgcg gtggcgtca gaccgcttt 
      gcaattctg tccaaaggc cgcgaaaaa gtaacttta 
      agtgcccaa cacctcctc tcgtgtgac cgaagaggc 
      caacagagc tgtaacggg ggttatttg gaccgggcc 
      tggtcttac atccgcaaa atcggattg gtcgacgag 
      cagtgcttc ccctacagt gcgaccaat gaaaagtgc 
      agaatccca agacgtggc gacttggtt actgccaac 
      tgtcaactt ccgaccaat gttgaccgc cgctcgaaa 
      tacaaggtg gccccagcg tacagagtg ggcaacgaa 
      accgacatc atgtacgaa attttgcat tccggccct 
      gttcaagcg acaatgaag gtctaccac gactttttc 
      acttacaag cgggggatt taccgccac tcgcccatc 
      tcgaccaac gaccgaacc ggctaccac tcggtgcga 
      atagtgggc tggggcgag gagtactcg cctgaaggg 
      ctcaaaaaa tactggaaa gtggccaac agttggggc 
      cccgaatgg ggcgaaaac ggctatttc cgcatcctt 
      cgtgggtcg aacgagtgc gagattgag tcgttcgtt 
      ttggggact tgggccgaa gtcgagaac aagttgctg 
      ttgcgcaac gagatttaa
    • >TC033581 RB
      atggttaag ttattaaag caatctgtg ctattttgg 
      gccttggtc ctcagcgtc aaaactgaa gatataaga 
      cgtaacaat tcaaaaaat aacaatcca ggttttttc 
      gaagcggag aacgtaaga aaactagaa actgatgaa 
      aattttgat gaacaatta aagttgggt tcacggttt 
      tcaccagag aatgaagaa aatcttcaa aggaaatgg 
      ggtcgtaaa attggttta gacgaaaac ttattgtct 
      cgtgatggt ctgaataaa cgtgacgga gaaaacgga 
      ggaaaaaat gaagaaaga tttgatgac aggcacata 
      aaaatgcat cgtccaaaa aatgcaaat caagagaga 
      aatacagaa gacagaaga agtaaaatt gaggaaagt 
      gatgctcgc cacaaattt atgaataaa caaagtgaa 
      aaattacaa agagggact gaagaaaga atcgacgac 
      agacaacca cacagatgt ggtcaaaaa ttgcatcgc 
      ccagaaaat gtaaatcga ggaaaaatt gataaagaa 
      aacataaga agccgtttc ttaaatgaa gcatcaaga 
      ttagaagaa catttcagg gacaacaat cctagatcc 
      agaagattt ggtctgaac acagaactt aacaaaaga 
      tttaatgaa attaattca cgtagaact ggtttaaac 
      gacagatta gctcgtaat gatcctcac aaattcaag 
      tttccaaca gatcaagcc gaaaaatta ggaagaaaa 
      aatcacaac agaattgaa aaaagacaa aaaggaaga 
      atgcagcgt ccagaagaa gctaatcta gagagaagt 
      acagacgac agaagaagt aaaattgaa gaaagtgat 
      gctcgccat agatttaat ccagaattt ctgaacgaa 
      caacacgaa aaattacaa agacggact gaggaaaga 
      aatgacgcc agacaattt cacagattc ggtcaaaaa 
      ttgcgacgt ccagagaat gtaaatctc ggaagacag 
      gagaagttg gaaaaatct ggattcaaa acgagtgtt 
      ggctgtgac aagtttcag atcagtgat atcaagcat 
      cgaggcggc gacgacgct ccgattatg gcctccttc 
      agctggctt tgccccctt gtggccctc accctcctc 
      accctaacc accgcccaa ttcagcgac tactctgat 
      ctccggggc ccttactgc caacggcgg ggctgttgc 
      cccggccgc caagacgat tgttccgag cctatttta 
      ggtacattg tgctactgt gacgatttc tgcaaccgg 
      acacgaaac gaagattgt tgtcccgac ttttggacg 
      cattgcaag ggccttccg cccccgccc gagccccca 
      tcccccatt ctagggtgc acgtacgaa gggagagtg 
      taccccctt aacaaacaa attaagaag aattgcaat 
      gtctgtaag tgtgaaaaa atgggccag aatcaggcc 
      gatatgttg tgcgagcag caccagtgt ttgattgaa 
      ccatcgatt acggaagca attaactct aattatgcc 
      aattatgga tggagtgct tcgaattat tcgaaattc 
      tggggccat aagctggaa gaagggatt aaattaagg 
      ttagggact ctccaaccg cagcgtttt gtcatgcac 
      atgaacccg gtgaggagg atttacgac ccaaattct 
      ctcccaaga gaattcgat tcggaattt aagtggcct 
      ggatggatg tcagaaata caggatcaa gggtggtgt 
      ggctcctcc tgggccata accacggct gcggtggcg 
      tcagaccgc tttgcaatt ctgtccaaa ggccgcgaa 
      aaagtaact ttaagtgcc caacacctc ctctcgtgt 
      gaccgaaga ggccaacag agctgtaac gggggttat 
      ttggaccgg gcctggtct tacatccgc aaaatcgga 
      ttggtcgac gagcagtgc ttcccctac agtgcgacc 
      aatgaaaag tgcagaatc ccaagacgt ggcgacttg 
      gttactgcc aactgtcaa cttccgacc aatgttgac 
      cgccgctcg aaatacaag gtggcccca gcgtacaga 
      gtgggcaac gaaaccgac atcatgtac gaaattttg 
      cattccggc cctgttcaa gcgacaatg aaggtctac 
      cacgacttt ttcacttac aagcggggg atttaccgc 
      cactcgccc atctcgacc aacgaccga accggctac 
      cactcggtg cgaatagtg ggctggggc gaggagtac 
      tcgcctgaa gggctcaaa aaatactgg aaagtggcc 
      aacagttgg ggccccgaa tggggcgaa aacggctat 
      ttccgcatc cttcgtggg tcgaacgag tgcgagatt 
      gagtcgttc gttttgggg acttgggcc gaagtcgag 
      aacaagttg ctgttgcgc aacgagatt taa
    mRNA sequence
    • >TC033581 RA
      catttcttg aagatggtt aagttatta aagcaatct 
      gtgctattt tgggccttg gtcctcagc gtcaaaact 
      gaagatata agacgtaac aattcaaaa aataacaat 
      ccaggtttt ttcgaagcg gagaacgta agaaaacta 
      gaaactgat gaaaatttt gatgaacaa ttaaagttg 
      ggttcacgg ttttcacca gagaatgaa gaaaatctt 
      caaaggaaa tggggtcgt aaaattggt ttagacgaa 
      aacttattg tctcgtgat ggtctgaat aaacgtgac 
      ggagaaaac ggaggaaaa aatgaagaa agatttgat 
      gacaggcac ataaaaatg catcgtcca aaaaatgca 
      aatcaagag agaaataca gaagacaga agaagtaaa 
      attgaggaa agtgatgct cgccacaaa tttatgaat 
      aaacaaagt gaaaaatta caaagaggg actgaagaa 
      agaatcgac gacagacaa ccacacaga tgtggtcaa 
      aaattgcat cgcccagaa aatgtaaat cgaggaaaa 
      attgataaa gaaaacata agaagccgt ttcttaaat 
      gaagcatca agattagaa gaacatttc agggacaac 
      aatcctaga tccagaaga tttggtctg aacacagaa 
      cttaacaaa agatttaat gaaattaat tcacgtaga 
      actggttta aacgacaga ttagctcgt aatgatcct 
      cacaaattc aagtttcca acagatcaa gccgaaaaa 
      ttaggaaga aaaaatcac aacagaatt gaaaaaaga 
      caaaaagga agaatgcag cgtccagaa gaagctaat 
      ctagagaga agtacagac gacagaaga agtaaaatt 
      gaagaaagt gatgctcgc catagattt aatccagaa 
      tttctgaac gaacaacac gaaaaatta caaagacgg 
      actgaggaa agaaatgac gccagacaa tttcacaga 
      ttcggtcaa aaattgcga cgtccagag aatgtaaat 
      ctcggaaga caggagaag ttggaaaaa tctggattc 
      aaaacgagt gttggctgt gacaagttt cagatcagt 
      gatatcaag catcgaggc ggcgacgac gctccgatt 
      atggcctcc ttcagctgg ctttgcccc cttgtggcc 
      ctcaccctc ctcacccta accaccgcc caattcagc 
      gactactct gatctccgg ggcccttac tgccaacgg 
      cggggctgt tgccccggc cgccaagac gattgttcc 
      gagcctatt ttaggcaat aacgaagtc atttccgaa 
      ggtacattg tgctactgt gacgatttc tgcaaccgg 
      acacgaaac gaagattgt tgtcccgac ttttggacg 
      cattgcaag ggccttccg cccccgccc gagccccca 
      tcccccatt ctagggtgc acgtacgaa gggagagtg 
      taccccctt aacaaacaa attaagaag aattgcaat 
      gtctgtaag tgtgaaaaa atgggccag aatcaggcc 
      gatatgttg tgcgagcag caccagtgt ttgattgaa 
      ccatcgatt acggaagca attaactct aattatgcc 
      aattatgga tggagtgct tcgaattat tcgaaattc 
      tggggccat aagctggaa gaagggatt aaattaagg 
      ttagggact ctccaaccg cagcgtttt gtcatgcac 
      atgaacccg gtgaggagg atttacgac ccaaattct 
      ctcccaaga gaattcgat tcggaattt aagtggcct 
      ggatggatg tcagaaata caggatcaa gggtggtgt 
      ggctcctcc tgggccata accacggct gcggtggcg 
      tcagaccgc tttgcaatt ctgtccaaa ggccgcgaa 
      aaagtaact ttaagtgcc caacacctc ctctcgtgt 
      gaccgaaga ggccaacag agctgtaac gggggttat 
      ttggaccgg gcctggtct tacatccgc aaaatcgga 
      ttggtcgac gagcagtgc ttcccctac agtgcgacc 
      aatgaaaag tgcagaatc ccaagacgt ggcgacttg 
      gttactgcc aactgtcaa cttccgacc aatgttgac 
      cgccgctcg aaatacaag gtggcccca gcgtacaga 
      gtgggcaac gaaaccgac atcatgtac gaaattttg 
      cattccggc cctgttcaa gcgacaatg aaggtctac 
      cacgacttt ttcacttac aagcggggg atttaccgc 
      cactcgccc atctcgacc aacgaccga accggctac 
      cactcggtg cgaatagtg ggctggggc gaggagtac 
      tcgcctgaa gggctcaaa aaatactgg aaagtggcc 
      aacagttgg ggccccgaa tggggcgaa aacggctat 
      ttccgcatc cttcgtggg tcgaacgag tgcgagatt 
      gagtcgttc gttttgggg acttgggcc gaagtcgag 
      aacaagttg ctgttgcgc aacgagatt taatcaaac 
      aagtgccat tgtgttcga aaaattgcc attttactt 
      cgtaactgc catgttatg tgttctgac aaattcatc 
      gcaactgtt ttttttatt tattgtgag gcgacgagc 
      gaaacaact caattttgt ggcttcgta tgctcgttg 
      tcacttcga tattacatt tattatgtt acgtgtgaa 
      tactaattc gctgttaaa tcatcgtat atttttata 
      tgtgtaaaa atatgaata aaattttta ctaattt
    • >TC033581 RB
      catttcttg aagatggtt aagttatta aagcaatct 
      gtgctattt tgggccttg gtcctcagc gtcaaaact 
      gaagatata agacgtaac aattcaaaa aataacaat 
      ccaggtttt ttcgaagcg gagaacgta agaaaacta 
      gaaactgat gaaaatttt gatgaacaa ttaaagttg 
      ggttcacgg ttttcacca gagaatgaa gaaaatctt 
      caaaggaaa tggggtcgt aaaattggt ttagacgaa 
      aacttattg tctcgtgat ggtctgaat aaacgtgac 
      ggagaaaac ggaggaaaa aatgaagaa agatttgat 
      gacaggcac ataaaaatg catcgtcca aaaaatgca 
      aatcaagag agaaataca gaagacaga agaagtaaa 
      attgaggaa agtgatgct cgccacaaa tttatgaat 
      aaacaaagt gaaaaatta caaagaggg actgaagaa 
      agaatcgac gacagacaa ccacacaga tgtggtcaa 
      aaattgcat cgcccagaa aatgtaaat cgaggaaaa 
      attgataaa gaaaacata agaagccgt ttcttaaat 
      gaagcatca agattagaa gaacatttc agggacaac 
      aatcctaga tccagaaga tttggtctg aacacagaa 
      cttaacaaa agatttaat gaaattaat tcacgtaga 
      actggttta aacgacaga ttagctcgt aatgatcct 
      cacaaattc aagtttcca acagatcaa gccgaaaaa 
      ttaggaaga aaaaatcac aacagaatt gaaaaaaga 
      caaaaagga agaatgcag cgtccagaa gaagctaat 
      ctagagaga agtacagac gacagaaga agtaaaatt 
      gaagaaagt gatgctcgc catagattt aatccagaa 
      tttctgaac gaacaacac gaaaaatta caaagacgg 
      actgaggaa agaaatgac gccagacaa tttcacaga 
      ttcggtcaa aaattgcga cgtccagag aatgtaaat 
      ctcggaaga caggagaag ttggaaaaa tctggattc 
      aaaacgagt gttggctgt gacaagttt cagatcagt 
      gatatcaag catcgaggc ggcgacgac gctccgatt 
      atggcctcc ttcagctgg ctttgcccc cttgtggcc 
      ctcaccctc ctcacccta accaccgcc caattcagc 
      gactactct gatctccgg ggcccttac tgccaacgg 
      cggggctgt tgccccggc cgccaagac gattgttcc 
      gagcctatt ttaggtaca ttgtgctac tgtgacgat 
      ttctgcaac cggacacga aacgaagat tgttgtccc 
      gacttttgg acgcattgc aagggcctt ccgcccccg 
      cccgagccc ccatccccc attctaggg tgcacgtac 
      gaagggaga gtgtacccc cttaacaaa caaattaag 
      aagaattgc aatgtctgt aagtgtgaa aaaatgggc 
      cagaatcag gccgatatg ttgtgcgag cagcaccag 
      tgtttgatt gaaccatcg attacggaa gcaattaac 
      tctaattat gccaattat ggatggagt gcttcgaat 
      tattcgaaa ttctggggc cataagctg gaagaaggg 
      attaaatta aggttaggg actctccaa ccgcagcgt 
      tttgtcatg cacatgaac ccggtgagg aggatttac 
      gacccaaat tctctccca agagaattc gattcggaa 
      tttaagtgg cctggatgg atgtcagaa atacaggat 
      caagggtgg tgtggctcc tcctgggcc ataaccacg 
      gctgcggtg gcgtcagac cgctttgca attctgtcc 
      aaaggccgc gaaaaagta actttaagt gcccaacac 
      ctcctctcg tgtgaccga agaggccaa cagagctgt 
      aacgggggt tatttggac cgggcctgg tcttacatc 
      cgcaaaatc ggattggtc gacgagcag tgcttcccc 
      tacagtgcg accaatgaa aagtgcaga atcccaaga 
      cgtggcgac ttggttact gccaactgt caacttccg 
      accaatgtt gaccgccgc tcgaaatac aaggtggcc 
      ccagcgtac agagtgggc aacgaaacc gacatcatg 
      tacgaaatt ttgcattcc ggccctgtt caagcgaca 
      atgaaggtc taccacgac tttttcact tacaagcgg 
      gggatttac cgccactcg cccatctcg accaacgac 
      cgaaccggc taccactcg gtgcgaata gtgggctgg 
      ggcgaggag tactcgcct gaagggctc aaaaaatac 
      tggaaagtg gccaacagt tggggcccc gaatggggc 
      gaaaacggc tatttccgc atccttcgt gggtcgaac 
      gagtgcgag attgagtcg ttcgttttg gggacttgg 
      gccgaagtc gagaacaag ttgctgttg cgcaacgag 
      atttaatca aacaagtgc cattgtgtt cgaaaaatt 
      gccatttta cttcgtaac tgccatgtt atgtgttct 
      gacaaattc atcgcaact gtttttttt atttattgt 
      gaggcgacg agcgaaaca actcaattt tgtggcttc 
      gtatgctcg ttgtcactt cgatattac atttattat 
      gttacgtgt gaatactaa ttcgctgtt aaatcatcg 
      tatattttt atatgtgta aaaatatga ataaaattt 
      ttactaatt t