Status
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Gene information
General information
TC033580
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009212,TC009213
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3069209..3077575
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033580
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01500

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033580 RA
      MKFSILLVA LWTTQLKAD DVAERGQRV ARQTSADQM 
      VQNILQWLQ GVYTQNSRK IRQGTLREY LPPSNTQKP 
      RPFEPQGYP PSGITPQPP FPIYTNPTT GYPSPTNEI 
      TPPHTGPPQ PPFTGYPTT GFPGSTGFP TQRPTGYPT 
      QRPTFPSQR PGFPTQPPT GYPTQRPGF PTQPPTGYP 
      TQRPTFPSQ RPGFPTQPP TGFPTTQPP TPAPTYGPP 
      ITTPQTSTG TPITSAPST HFTSPAITT EATPSPEDN 
      NIVPTTRPG PTEIPQTPQ PGVPTDEDL KHPPHIHAI 
      DVECAKDMM TITIEFNRE FNGVIYSKG YYNMPECRY 
      VNENSEMTK YRFTVSLNS CGTEFINAF DTQGQSYLE 
      NVLVIQNEQ GIQEVWDTI RAVRCLWEG NLKDSLSVA 
      LSVGMLSQE IVTFSGDTA MAKLDIQLG KGPFAPPAN 
      GLVKIGEQM TLVISVTGD PGFDIQVKD CRATDSTGD 
      RVYPLTDEN GCILKPKLF GAFQKTRNT GNTGASIIA 
      YAFFNAFKF PDVMDLMIQ CNIELCKTD CQVCPEPNQ 
      QIEPGRRRK RDLSYYNNT DGVTMGKHL RVVLAEDLN 
      DIGNTEAAF EGEVCMTTQ NFLLVSAIL ISLLTASSV 
      LTLCHLPKK RQMANHYEV PNWAGKPPV GLHLDVLKG 
      DKLIQKLMI DDKKCYLFG RNAQMNDFC IDHASCSRV 
      HAALVWHKH LDRAFLVDL GSTHGTFIG SLRLEAYKP 
      TQLPIDSSF HFGASTRNY TIRERPQTG MRPIIDEIE 
      KAGEETEGG LLGLPETET ELDNLTEFN TAHNRRISM 
      LGITDDTEK RNTNRKRKR QVVSFNEDE EIINPEDVD 
      PSIGKFRNL VQTTVVPST IKRAKFAAE NIGIPTEYK 
      NPKILHPSA PDLYTDLPP ESHSPTSSG NLSIYSSLS 
      SRLGMVLPN PAPDVDMGQ NFAPPTPFV PPQVQIADA 
      MEPKKKKYA KEAWPGKKP TQTLLV
    CDS sequence
    • >TC033580 RA
      atgaaattt tccattctg ttggtcgcc ctgtggacg 
      acccagctt aaggccgac gatgttgcg gaaagagga 
      caaagagtc gcaaggcaa accagtgct gatcaaatg 
      gtccaaaac atccttcaa tggcttcaa ggagtctac 
      acacagaac agtaggaaa attcgccaa ggcactctt 
      cgtgaatac ctgcctccg agcaacacg caaaaacca 
      cgaccattc gaaccacaa ggctacccc ccatctggg 
      atcaccccc cagcccccg ttccccatc tacaccaac 
      cccacgaca ggatacccc tcccccacc aacgagata 
      accccacct cacaccggc cccccgcaa ccccctttc 
      acaggatac cccaccacg ggattcccc ggaagcacg 
      ggattccca acccaacgc ccgacaggg taccccacc 
      cagagaccc accttcccg tcccaaaga cccggattt 
      cccacacaa ccgccgaca gggtacccc acccaaagg 
      ccgggattc cccacacaa cccccgaca gggtacccc 
      acacaaaga cccactttc ccctcccaa aggccggga 
      ttccccaca caacctcca accggattc cccaccact 
      caacctccc actcctgca ccgacgtac ggtccacca 
      atcacaaca cctcaaacc tccactggt accccaata 
      acctcggcc ccctccact cactttacc tcccctgcc 
      ataacaacc gaagccacg ccctcgccc gaggataac 
      aacatagtg ccaaccacg cggcctgga cccacggaa 
      atcccccaa acgccacag ccaggcgta ccaactgat 
      gaggatttg aaacacccg ccacatatt cacgcaatt 
      gatgtcgag tgcgccaaa gacatgatg accatcacg 
      attgaattt aaccgcgag ttcaatgga gtgatttac 
      tccaaaggc tactacaac atgcctgaa tgtcgctac 
      gtgaacgaa aattccgaa atgacgaaa taccgcttc 
      accgtcagt ttgaactct tgtgggact gaatttatc 
      aacgcgttt gatactcaa ggacagtcg tacctggag 
      aacgtcctc gtgatccaa aacgagcaa gggatccag 
      gaggtttgg gacacaatc agagctgtg aggtgcctc 
      tgggagggc aacctgaag gactccctc agcgtggcg 
      ctcagcgtg gggatgcta agccaggag attgtgact 
      ttcagtggg gacaccgcc atggccaag ctggacatc 
      caactgggc aaaggccca ttcgcgccc ccggccaac 
      ggtttggtc aaaatcggg gagcaaatg acccttgtt 
      atctccgtg accggagac cccggtttt gacatccaa 
      gtcaaagac tgcagggct acggactcg accggcgac 
      cgcgtgtac cccctaacc gacgaaaac ggctgtatt 
      ctcaaaccc aaactattt ggcgcgttc caaaaaaca 
      cgaaacacg ggaaatacc ggcgcttcc atcattgcc 
      tacgcgttt ttcaacgct tttaaattc ccagatgtg 
      atggatttg atgattcag tgcaacatt gagctttgc 
      aaaaccgac tgtcaggtg tgtccagag cccaaccag 
      caaatcgag cctgggagg aggcgcaaa cgtgacctt 
      tcgtactat aataacact gacggggtc acaatgggg 
      aagcacctg agggtcgtt ttggcggag gatttgaac 
      gatattggc aatactgag gccgcgttt gagggggaa 
      gtttgtatg acgacgcag aattttcta ctagtttcg 
      gcgattttg atctcgcta ctcactgcc agtagtgta 
      ctaacgtta tgtcacctg ccaaaaaag cgacaaatg 
      gcaaaccat tatgaggtg ccaaattgg gcaggcaag 
      ccccccgtg ggtttgcat ttggacgtg ctaaagggc 
      gacaaacta atacaaaaa ttgatgatc gacgacaaa 
      aagtgttac ttatttgga cgcaacgcc caaatgaac 
      gatttctgc atcgatcac gcgtcatgt tcgcgggtt 
      catgcagcc ctcgtttgg cacaaacac ttagatcgg 
      gcgtttctg gtggatttg gggagcacc catggcacc 
      ttcatagga tcattaaga cttgaggcg tataaaccc 
      actcagttg cctattgat tctagtttt cactttggg 
      gcgtccacg cgaaattac acgatcagg gagcggccc 
      cagacgggc atgcggccg atcattgac gagatcgaa 
      aaggcgggc gaggagacc gagggcgga ttgctgggg 
      ctccccgag accgaaacg gaactcgat aatttgacg 
      gaatttaac accgcccac aaccggcgg atatcaatg 
      ctgggaatc accgacgac acggaaaaa cgcaacacc 
      aaccggaag cgcaaacgc caagtcgtt tccttcaac 
      gaggacgaa gagatcata aaccctgaa gatgtggac 
      ccgagtatc ggtaaattc cgaaacttg gtccaaacc 
      accgttgta ccttcaacc atcaagagg gccaaattc 
      gcggctgag aatatcgga atccccact gaatacaaa 
      aatcccaaa atccttcat ccctccgcc cccgatttg 
      tacacagac ctgcccccc gaatcccac tctccaacc 
      agttcaggg aatttaagc atttacagt tcgttaagt 
      tcacgcctt ggaatggtt ttacccaat ccggcgcca 
      gatgtcgac atgggacag aacttcgcc ccgcccact 
      ccctttgtc ccgccccaa gtacaaatt gcggacgcc 
      atggagccc aagaaaaag aagtatgcc aaggaggcg 
      tggcctggc aagaagccc acgcaaacg ctattggtc 
      tag
    mRNA sequence
    • >TC033580 RA
      gagcatcaa taagtgtgc gtatataaa aggcaaatg 
      agagaagat gcacttagt tagttttta gttttcttt 
      tgtgtttag gataacttt ggcgcagaa tgaaatttt 
      ccattctgt tggtcgccc tgtggacga cccagctta 
      aggccgacg atgttgcgg aaagaggac aaagagtcg 
      caaggcaaa ccagtgctg atcaaatgg tccaaaaca 
      tccttcaat ggcttcaag gagtctaca cacagaaca 
      gtaggaaaa ttcgccaag gcactcttc gtgaatacc 
      tgcctccga gcaacacgc aaaaaccac gaccattcg 
      aaccacaag gctaccccc catctggga tcacccccc 
      agcccccgt tccccatct acaccaacc ccacgacag 
      gatacccct cccccacca acgagataa ccccacctc 
      acaccggcc ccccgcaac cccctttca caggatacc 
      ccaccacgg gattccccg gaagcacgg gattcccaa 
      cccaacgcc cgacagggt accccaccc agagaccca 
      ccttcccgt cccaaagac ccggatttc ccacacaac 
      cgccgacag ggtacccca cccaaaggc cgggattcc 
      ccacacaac ccccgacag ggtacccca cacaaagac 
      ccactttcc cctcccaaa ggccgggat tccccacac 
      aacctccaa ccggattcc ccaccactc aacctccca 
      ctcctgcac cgacgtacg gtccaccaa tcacaacac 
      ctcaaacct ccactggta ccccaataa cctcggccc 
      cctccactc actttacct cccctgcca taacaaccg 
      aagccacgc cctcgcccg aggataaca acatagtgc 
      caaccacgc ggcctggac ccacggaaa tcccccaaa 
      cgccacagc caggcgtac caactgatg aggatttga 
      aacacccgc cacatattc acgcaattg atgtcgagt 
      gcgccaaag acatgatga ccatcacga ttgaattta 
      accgcgagt tcaatggag tgatttact ccaaaggct 
      actacaaca tgcctgaat gtcgctacg tgaacgaaa 
      attccgaaa tgacgaaat accgcttca ccgtcagtt 
      tgaactctt gtgggactg aatttatca acgcgtttg 
      atactcaag gacagtcgt acctggaga acgtcctcg 
      tgatccaaa acgagcaag ggatccagg aggtttggg 
      acacaatca gagctgtga ggtgcctct gggagggca 
      acctgaagg actccctca gcgtggcgc tcagcgtgg 
      ggatgctaa gccaggaga ttgtgactt tcagtgggg 
      acaccgcca tggccaagc tggacatcc aactgggca 
      aaggcccat tcgcgcccc cggccaacg gtttggtca 
      aaatcgggg agcaaatga cccttgtta tctccgtga 
      ccggagacc ccggttttg acatccaag tcaaagact 
      gcagggcta cggactcga ccggcgacc gcgtgtacc 
      ccctaaccg acgaaaacg gctgtattc tcaaaccca 
      aactatttg gcgcgttcc aaaaaacac gaaacacgg 
      gaaataccg gcgcttcca tcattgcct acgcgtttt 
      tcaacgctt ttaaattcc cagatgtga tggatttga 
      tgattcagt gcaacattg agctttgca aaaccgact 
      gtcaggtgt gtccagagc ccaaccagc aaatcgagc 
      ctgggagga ggcgcaaac gtgaccttt cgtactata 
      ataacactg acggggtca caatgggga agcacctga 
      gggtcgttt tggcggagg atttgaacg atattggca 
      atactgagg ccgcgtttg agggggaag tttgtatga 
      cgacgcaga attttctac tagtttcgg cgattttga 
      tctcgctac tcactgcca gtagtgtac taacgttat 
      gtcacctgc caaaaaagc gacaaatgg caaaccatt 
      atgaggtgc caaattggg caggcaagc cccccgtgg 
      gtttgcatt tggacgtgc taaagggcg acaaactaa 
      tacaaaaat tgatgatcg acgacaaaa agtgttact 
      tatttggac gcaacgccc aaatgaacg atttctgca 
      tcgatcacg cgtcatgtt cgcgggttc atgcagccc 
      tcgtttggc acaaacact tagatcggg cgtttctgg 
      tggatttgg ggagcaccc atggcacct tcataggat 
      cattaagac ttgaggcgt ataaaccca ctcagttgc 
      ctattgatt ctagttttc actttgggg cgtccacgc 
      gaaattaca cgatcaggg agcggcccc agacgggca 
      tgcggccga tcattgacg agatcgaaa aggcgggcg 
      aggagaccg agggcggat tgctggggc tccccgaga 
      ccgaaacgg aactcgata atttgacgg aatttaaca 
      ccgcccaca accggcgga tatcaatgc tgggaatca 
      ccgacgaca cggaaaaac gcaacacca accggaagc 
      gcaaacgcc aagtcgttt ccttcaacg aggacgaag 
      agatcataa accctgaag atgtggacc cgagtatcg 
      gtaaattcc gaaacttgg tccaaacca ccgttgtac 
      cttcaacca tcaagaggg ccaaattcg cggctgaga 
      atatcggaa tccccactg aatacaaaa atcccaaaa 
      tccttcatc cctccgccc ccgatttgt acacagacc 
      tgccccccg aatcccact ctccaacca gttcaggga 
      atttaagca tttacagtt cgttaagtt cacgccttg 
      gaatggttt tacccaatc cggcgccag atgtcgaca 
      tgggacaga acttcgccc cgcccactc cctttgtcc 
      cgccccaag tacaaattg cggacgcca tggagccca 
      agaaaaaga agtatgcca aggaggcgt ggcctggca 
      agaagccca cgcaaacgc tattggtct aggattagg 
      tgtaagctt ttgatgtaa ttttctcaa ggtcataga 
      ttaaatatt caataaaaa acgaaaagt agt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY