Status
Please wait ...
Gene information
General information
TC009208 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3091468..3094995
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009208
    iBeetle screen iB_01499

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Adult morphology
    abdominal odoriferous glands colour darker - Number of animals showing the phenotype: 10 (13 dpi)
    Days post injection



    abdominal odoriferous glands partially content darker - Number of animals showing the phenotype: 10 (13 dpi)
    Days post injection



    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 10 (13 dpi)
    Days post injection


    abdominal odoriferous glands content necrotic - Number of animals showing the phenotype: 10 (13 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009208 RA
      MFRRLLQMA GLGSSCTRD CSGRGECNN GTCMCDIRY 
      TGELCDGPN LPYHAGIGG VFFLVGLVC AIQLIMCVM 
      SEYQRLKAP TFLKACRIT TQKAIYFVM CIASLIRGA 
      YFISPDSFE YGWSSSLMS SYYPLLMSS SSLIVCFWA 
      EVFHLQGIR WEKSQFLSK SLLGFVTFN IISYSLLLA 
      EVITTTLAE PPPHDPGFY RHLFNGCYA VLLFIVVVF 
      FLIYGVEVF FKVRGGFVG KPLHLAGGG ESEPLRAEE 
      LRPQINLSQ LHQSRIGLL SQALMLITI VGFLFSETL 
      SEFWKTKVP IVSRNWHDV VFRVVEIGV AVWFPAVLW 
      NCMQPSELW ILNPQKLLA KLDQKPQEV VASASTMTA 
      DDEAFLDKR ECWICYDID KPEPLIQPC ACTGDVSSV 
      HHDCLRRWL MESSAGSGE ALKCKVCNY EYEVCSTTK 
      LDWERGFTS KHWGCNAVV VTCLCVVVA VAWIIIQLY 
      DNSYIRMLS ASVALLVIY ICIKFLGQT TLSAYQRAK 
      VAALNIGGH VTTISGAVN IETNDSQPQ AAL
    CDS sequence
    • >TC009208 RA
      atgtttcga cgtttgctc caaatggca ggtctaggc 
      tcttcctgt accagggac tgttccggc cgtggggag 
      tgcaacaac ggcacttgc atgtgcgac attcgctac 
      acgggggag ttgtgcgat ggccccaat ttgccctac 
      catgcgggc attgggggt gtctttttc ctggtgggg 
      ctcgtgtgc gctatacag ctgatcatg tgcgtcatg 
      tcggagtac cagcgcctg aaagccccc acgtttctc 
      aaagcttgc cggattacc actcagaag gcgatttat 
      ttcgtcatg tgcattgcc agcttgatc agaggggct 
      tacttcata tccccggac tcgttcgag tatggctgg 
      tcttcgagt ttgatgtcc tcttattat cctctcctc 
      atgtccagt tcatctcta atagtgtgc ttctgggct 
      gaggtgttt catttgcaa gggattcgt tgggagaag 
      tcgcagttt ctgtcgaag tctcttctg ggttttgtc 
      acgtttaat atcatcagt tatagttta ttgttggcg 
      gaagtgatt acaactaca ctggctgaa ccaccacct 
      catgatcca ggattctac agacatcta ttcaacggt 
      tgttacgca gttttgctt tttatagtt gtggtgttt 
      ttcctgatt tacggcgtc gaagtgttc tttaaggtc 
      cgcggcggt ttcgtgggc aaacccctg catctagcg 
      ggcggtggt gaatccgaa ccgctccgt gccgaagag 
      ctccgcccc caaatcaac ctctcgcaa ctccaccaa 
      tcccgtatc ggtctgctc agccaagcg ctaatgctc 
      atcaccatc gtcggtttc ctcttctct gaaaccctt 
      tccgaattt tggaaaacc aaagtcccc atagtttcg 
      cgcaattgg cacgacgtc gtcttcaga gtggtggag 
      atcggcgta gcggtgtgg tttcctgcg gttttgtgg 
      aactgcatg cagccgtcc gagctatgg atcctcaat 
      ccgcaaaag ctgctagcg aagctggac cagaagcct 
      caggaggtg gtggcgagc gcgtcgacg atgacggcc 
      gacgatgag gcgtttttg gataagagg gagtgctgg 
      atttgctac gatattgat aagccggaa ccgttgatt 
      cagccgtgt gcgtgcact ggggatgtg tccagtgtg 
      catcatgat tgcctgagg aggtggctg atggagagt 
      tcggcaggg agtggcgaa gcgctgaag tgtaaagtc 
      tgcaattac gagtatgag gtttgcagt acgaccaag 
      ttggactgg gagaggggt ttcacgtcc aagcattgg 
      ggttgcaat gctgttgtt gttacatgt ctttgcgtg 
      gtggtcgca gtggcctgg attattata caactgtat 
      gataatagt tacatcagg atgctgagc gcgagtgtg 
      gcgctactg gtcatctat atctgcatc aaatttttg 
      gggcagact acgttgagc gcctaccag agggccaaa 
      gtggccgct ttgaacatc ggcggtcac gtgacgacg 
      atcagtgga gccgtcaat atcgaaaca aacgattct 
      cagccgcaa gcagcattg taa
    mRNA sequence
    • >TC009208 RA
      cgatcggtg tcagatttt gacatattt acgattatg 
      ttgaattgt gtggcttgt gtcgagcgc ttaaagcag 
      tgaaatcgt aaatattgt caattttgt gtttagtgg 
      tgtgttggg gagcccgtt gtgcgaaga ctgaataat 
      gtttcgacg tttgctcca aatggcagg tctaggctc 
      ttcctgtac cagggactg ttccggccg tggggagtg 
      caacaacgg cacttgcat gtgcgacat tcgctacac 
      gggggagtt gtgcgatgg ccccaattt gccctacca 
      tgcgggcat tgggggtgt ctttttcct ggtggggct 
      cgtgtgcgc tatacagct gatcatgtg cgtcatgtc 
      ggagtacca gcgcctgaa agcccccac gtttctcaa 
      agcttgccg gattaccac tcagaaggc gatttattt 
      cgtcatgtg cattgccag cttgatcag aggggctta 
      cttcatatc cccggactc gttcgagta tggctggtc 
      ttcgagttt gatgtcctc ttattatcc tctcctcat 
      gtccagttc atctctaat agtgtgctt ctgggctga 
      ggtgtttca tttgcaagg gattcgttg ggagaagtc 
      gcagtttct gtcgaagtc tcttctggg ttttgtcac 
      gtttaatat catcagtta tagtttatt gttggcgga 
      agtgattac aactacact ggctgaacc accacctca 
      tgatccagg attctacag acatctatt caacggttg 
      ttacgcagt tttgctttt tatagttgt ggtgttttt 
      cctgattta cggcgtcga agtgttctt taaggtccg 
      cggcggttt cgtgggcaa acccctgca tctagcggg 
      cggtggtga atccgaacc gctccgtgc cgaagagct 
      ccgccccca aatcaacct ctcgcaact ccaccaatc 
      ccgtatcgg tctgctcag ccaagcgct aatgctcat 
      caccatcgt cggtttcct cttctctga aaccctttc 
      cgaattttg gaaaaccaa agtccccat agtttcgcg 
      caattggca cgacgtcgt cttcagagt ggtggagat 
      cggcgtagc ggtgtggtt tcctgcggt tttgtggaa 
      ctgcatgca gccgtccga gctatggat cctcaatcc 
      gcaaaagct gctagcgaa gctggacca gaagcctca 
      ggaggtggt ggcgagcgc gtcgacgat gacggccga 
      cgatgaggc gtttttgga taagaggga gtgctggat 
      ttgctacga tattgataa gccggaacc gttgattca 
      gccgtgtgc gtgcactgg ggatgtgtc cagtgtgca 
      tcatgattg cctgaggag gtggctgat ggagagttc 
      ggcagggag tggcgaagc gctgaagtg taaagtctg 
      caattacga gtatgaggt ttgcagtac gaccaagtt 
      ggactggga gaggggttt cacgtccaa gcattgggg 
      ttgcaatgc tgttgttgt tacatgtct ttgcgtggt 
      ggtcgcagt ggcctggat tattataca actgtatga 
      taatagtta catcaggat gctgagcgc gagtgtggc 
      gctactggt catctatat ctgcatcaa atttttggg 
      gcagactac gttgagcgc ctaccagag ggccaaagt 
      ggccgcttt gaacatcgg cggtcacgt gacgacgat 
      cagtggagc cgtcaatat cgaaacaaa cgattctca 
      gccgcaagc agcattgta attgtttta aaaatgtga 
      tatttatcg atgtattat tattagccg gattcaata 
      ttgtgaact gttcacata ctatatttt tttcgtact 
      aattataga ttattggta ggaagcatg ttattgtcg 
      tttttaagt atatttact tagttgttg gaattttat 
      ttactgaag tgagatgcc aagatgtat atatttcgt 
      tctcactgt gtagatgta gcgattgac ctgttcaag 
      gttaggagt actatcact tttttgtgt acataggat 
      tatgcgatt tttgtcgtt agaatttta attttgttg 
      agttaggaa ttgcagatc tcgaggaaa ttttgttta 
      ttcgttccc aacagctgt gttaatgta tcatgtttc 
      gtggtagaa ataaaagaa attctcagc g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY