Status
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Gene information
General information
TC009205 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3167411..3169324
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009205
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01497

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009205 RA
      MPDIKITPL GAGQDVGRS CILLTMGGK NIMLDCGMH 
      MGYNDERRF PDFSYISQE GPLTSYIDC VIISHFHLD 
      HCGALPYMS EMVGYSGPI YMTHPTKAI APILLEDMR 
      KVSVEKKGD QNFFTSQMI KDCMKKVIA VTLHQSLMV 
      DNEIEIKAY YAGHVLGAA MFWIRVGAQ SVVYTGDYN 
      MTPDRHLGA AWIDKCRPD LLISESTYA TTIRDSKRC 
      RERDFLKKV HECMDRGGK VLIPVFALG RAQELCILL 
      ETYWERMNL KAPVYFALG LTEKANNYY KMFITWTNQ 
      KIRKTFVQR NMFDFKHIK PFDRSYIDN PGPMVVFAT 
      PGMLHAGLS LQIFKKWAP NENNMVIMP GFCVQGTVG 
      HKILNGAKR VEFENKQIV EVKMSVEYM SFSAHADAK 
      GIMQLIQHC EPRNVMLVH GEAEKMEFL KQKILQEFS 
      INCYNPANG ETCVISTPV KIPIDVSLP LLKSEARKF 
      SALPPDPKR RRTLHGVLV MKENNVCLM DVDDACKEA 
      GINRHIIRF SSTVHLNDT GPALATAHK LHGFIKENL 
      PQWPVVFAE GEISIESVL VKVEGDEDE QKNVYVSWT 
      NQDEDLGSY ILNLLNRMG Q
    CDS sequence
    • >TC009205 RA
      atgccggat atcaagata acccctttg ggggccggc 
      caggacgtg ggccgcagc tgcatcctc ctcacaatg 
      gggggcaaa aacataatg ttggactgc ggcatgcac 
      atggggtac aacgacgag cgccgcttc cccgacttc 
      tcctacata tctcaagaa ggtcccctt acctcctac 
      atcgactgt gtaattatc tcccatttc catctggac 
      cattgcggg gctttgccc tacatgtcc gaaatggtg 
      ggttactca ggccccatc tacatgact caccccact 
      aaagctatc gcccccatt ctcctggaa gacatgcga 
      aaagtctca gttgagaaa aaaggtgac cagaatttt 
      ttcacgtcc caaatgatc aaagactgt atgaagaaa 
      gtcattgct gtgacttta caccagtct ttgatggtg 
      gacaacgag attgaaatt aaagcgtat tatgcggga 
      catgtcctg ggggctgcc atgttctgg atacgcgtg 
      ggggcccag tctgtggtg tacaccggc gattacaac 
      atgaccccc gaccggcat ctgggggct gcctggatt 
      gataaatgt cgccccgat ttgttaatc tccgaatcg 
      acatacgcc actaccatt cgagattcg aaacgttgt 
      cgcgaacgc gactttttg aaaaaagtc cacgagtgt 
      atggaccgt gggggcaaa gtgctaatc cccgtgttt 
      gcattagga cgtgcccaa gaactttgt atccttctg 
      gagacatac tgggaacgt atgaatctc aaagccccc 
      gtgtacttt gccctagga ctcaccgaa aaggcgaac 
      aactactac aaaatgttt ataacttgg acgaatcaa 
      aaaatccga aaaacgttc gtgcaacgt aacatgttc 
      gatttcaag cacatcaag cctttcgac cgttcgtat 
      attgataat ccgggcccc atggtggtt ttcgccaca 
      cctgggatg ctccacgcg gggctgagc ctccaaatc 
      ttcaaaaag tgggccccc aatgagaac aacatggtc 
      ataatgccc ggtttttgt gtccaaggc accgtgggc 
      cataaaatt ctgaatggg gcgaaacga gtcgaattc 
      gaaaataag caaatcgtg gaggtgaaa atgtccgtt 
      gaatacatg agtttctcg gcacatgct gatgccaaa 
      ggcatcatg caactgatc caacattgc gaacctcga 
      aacgttatg cttgtccac ggcgaggcc gagaaaatg 
      gagtttctc aagcagaaa atcctccaa gagttcagt 
      attaactgc tataacccc gccaatggc gagacttgt 
      gttatttca acaccggtc aaaatccca atcgatgtt 
      tcgttgccg ctgctcaag tccgaagcg aggaaattc 
      agcgctttg ccgccggac cccaaacgg aggcgcacc 
      ctgcacgga gtgctcgtc atgaaggag aacaatgtg 
      tgtttgatg gacgtggac gacgcgtgt aaggaggct 
      gggattaat aggcatata attcggttt tcgtcaacg 
      gttcatttg aatgataca gggccggcg ttagcaacg 
      gcccataaa ttacacggt ttcattaaa gagaatttg 
      ccacaatgg ccggtggtg ttcgccgaa ggcgagatt 
      tcaatcgag tcggttctg gtcaaggtt gaaggcgat 
      gaggacgaa caaaagaac gtctacgtc tcgtggacc 
      aatcaggac gaggatttg ggcagttat atattgaat 
      ttgttgaat cgaatggga caataa
    mRNA sequence
    • >TC009205 RA
      gtaacgtaa cctcaaatg ctctgctta gttttttgg 
      tgtttttaa ccctctaat gccggatat caagataac 
      ccctttggg ggccggcca ggacgtggg ccgcagctg 
      catcctcct cacaatggg gggcaaaaa cataatgtt 
      ggactgcgg catgcacat ggggtacaa cgacgagcg 
      ccgcttccc cgacttctc ctacatatc tcaagaagg 
      tccccttac ctcctacat cgactgtgt aattatctc 
      ccatttcca tctggacca ttgcggggc tttgcccta 
      catgtccga aatggtggg ttactcagg ccccatcta 
      catgactca ccccactaa agctatcgc ccccattct 
      cctggaaga catgcgaaa agtctcagt tgagaaaaa 
      aggtgacca gaatttttt cacgtccca aatgatcaa 
      agactgtat gaagaaagt cattgctgt gactttaca 
      ccagtcttt gatggtgga caacgagat tgaaattaa 
      agcgtatta tgcgggaca tgtcctggg ggctgccat 
      gttctggat acgcgtggg ggcccagtc tgtggtgta 
      caccggcga ttacaacat gacccccga ccggcatct 
      gggggctgc ctggattga taaatgtcg ccccgattt 
      gttaatctc cgaatcgac atacgccac taccattcg 
      agattcgaa acgttgtcg cgaacgcga ctttttgaa 
      aaaagtcca cgagtgtat ggaccgtgg gggcaaagt 
      gctaatccc cgtgtttgc attaggacg tgcccaaga 
      actttgtat ccttctgga gacatactg ggaacgtat 
      gaatctcaa agcccccgt gtactttgc cctaggact 
      caccgaaaa ggcgaacaa ctactacaa aatgtttat 
      aacttggac gaatcaaaa aatccgaaa aacgttcgt 
      gcaacgtaa catgttcga tttcaagca catcaagcc 
      tttcgaccg ttcgtatat tgataatcc gggccccat 
      ggtggtttt cgccacacc tgggatgct ccacgcggg 
      gctgagcct ccaaatctt caaaaagtg ggcccccaa 
      tgagaacaa catggtcat aatgcccgg tttttgtgt 
      ccaaggcac cgtgggcca taaaattct gaatggggc 
      gaaacgagt cgaattcga aaataagca aatcgtgga 
      ggtgaaaat gtccgttga atacatgag tttctcggc 
      acatgctga tgccaaagg catcatgca actgatcca 
      acattgcga acctcgaaa cgttatgct tgtccacgg 
      cgaggccga gaaaatgga gtttctcaa gcagaaaat 
      cctccaaga gttcagtat taactgcta taaccccgc 
      caatggcga gacttgtgt tatttcaac accggtcaa 
      aatcccaat cgatgtttc gttgccgct gctcaagtc 
      cgaagcgag gaaattcag cgctttgcc gccggaccc 
      caaacggag gcgcaccct gcacggagt gctcgtcat 
      gaaggagaa caatgtgtg tttgatgga cgtggacga 
      cgcgtgtaa ggaggctgg gattaatag gcatataat 
      tcggttttc gtcaacggt tcatttgaa tgatacagg 
      gccggcgtt agcaacggc ccataaatt acacggttt 
      cattaaaga gaatttgcc acaatggcc ggtggtgtt 
      cgccgaagg cgagatttc aatcgagtc ggttctggt 
      caaggttga aggcgatga ggacgaaca aaagaacgt 
      ctacgtctc gtggaccaa tcaggacga ggatttggg 
      cagttatat attgaattt gttgaatcg aatgggaca 
      ataatttat ttttgttga gatttggtg ttttattta 
      cccccaata cacgaaaat ttaaaagaa attgtttca 
      atttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY