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Gene information
General information
TC009196 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 1157745..1163914
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009196
    iBeetle screen iB_01494
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 abdomen musculature partially randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009196 RA
      MEKSIGDNG DEISTSSVS SSESINFAS KLPQAELNR 
      IASKPIVFS WRPPDFPED SLDNASSQC MLFDSPLSE 
      AEKSKVITQ EPTVPPDTW LLGGTLGNR HATLVFKSS 
      ALANCTQKS IQEKPGHKL HITYKVLQA ETKLLSKLL 
      ECHGIVEVA PNSSDFNLL WTGSHPKPG ALRSLAPHQ 
      RVNHFPRSY ELTRKDRLY KNIERMQHC KGYKHFDFI 
      PQTFVMPTE FGELCSTHH RIKGPWIVK PVASSRGRG 
      IFIVETPNQ VPLEEPVVV AKYISRPLL VAGHKCDLR 
      LYVAVTSFD PLLVYIYEE GLVRFATVK YDSSHKQLW 
      NPCMHLCNY SINKYHSDY VKSDDPSAE NVGHKWTLS 
      ALLRHLKAE GKNTAVLMS QVEDLVIKA ILASANSII 
      SACKMFVPN PNNCFELYG FDILIDANL KPWLLEVNL 
      SPSLGCDSP LDVRLKSAM LSDLLTLVG IPAVDPILR 
      PTTNSLTSA SIKSSSRVK LGHCRRVHS ADGLSSTLP 
      RKNSSIHGD RLSSSALTL TPEETRAIK LAKSQFERR 
      GGFVRIFPA LDSWAKYSQ YLDPATGIP ISGNNNSYT 
      SYNLSGAHN YNLLLYTHL FPDAPVNTK LTDRSSLRY 
      SSLDRKRDL SLDSHTGSS LARQDRYER QLSQGHRQS 
      LAPQKQLKE GRSIELRKQ IVDMLRNGK KMTQSETRK 
      TFSHYLVCV LRRISSGPD QEDHMEIVL KFLQKASSY 
      LRTPYQVKT PSQSLDYKD RAAVVAKQL NDFLYLYNR 
      ETELFVDKT EKPSQIPAK LYAEFLENA RECDLEEIL 
      VHQTTQTVP THGGRTGFQ GVLKCIPNV PKSCGLKPP 
      YKTTTKGKG VKVASLS
    CDS sequence
    • >TC009196 RA
      atggaaaaa agtatcgga gacaacggc gacgagata 
      tcaacgtca agtgtgagc agttcggag tcgatcaat 
      ttcgcgtca aagttgccc caagctgaa ttaaatcgt 
      atcgcgtcc aaacctatc gtgttctcc tggcgcccc 
      ccagacttc cccgaagac tccctcgac aatgcctcg 
      tcccagtgc atgctgttc gacagcccc ctttcggag 
      gcggaaaag agcaaagtg ataacccaa gaaccgaca 
      gtgcccccc gacacgtgg ctcctcggt ggcaccctc 
      ggcaatcgt cacgcaact ctagttttt aaaagttca 
      gctctggca aactgtact caaaaatca attcaagaa 
      aaaccaggc cacaaactt cacatcacg tacaaagta 
      cttcaagcc gaaacgaaa ctcctttca aagttattg 
      gaatgtcat ggaatcgtt gaagttgca ccgaactcg 
      tccgatttt aatctttta tggacgggt tcgcacccg 
      aaaccgggc gctttgcga tcgttagcc ccccatcag 
      cgggtcaat cattttcca cgctcttac gaattgacc 
      aggaaagac agattgtat aaaaatatc gagcggatg 
      cagcactgc aaaggctac aaacatttc gattttatt 
      ccgcaaact tttgtaatg ccgaccgaa tttggggag 
      ttgtgctcg acgcatcat cgcatcaag gggccctgg 
      atagtcaag ccagtggcg tcgagtcgg gggcgaggt 
      atttttatt gtcgaaaca ccaaatcaa gtgcctctg 
      gaagagccg gtcgttgtg gccaagtat atatcgcga 
      ccgttgctt gtcgctggt cacaaatgt gatttgcga 
      ttatacgtc gctgttacg tcgtttgat cctttgctt 
      gtttatatt tacgaagaa ggtttagtc agatttgct 
      accgtcaag tacgattct agtcataaa cagctgtgg 
      aatccctgt atgcatctg tgtaattat agcataaat 
      aagtatcat agtgattat gtcaaatcg gacgacccg 
      agcgccgaa aacgtgggt cataagtgg acactgagt 
      gcccttttg cgacatttg aaagccgag gggaaaaac 
      acggcggtt ttaatgtca caggtcgag gatttggtc 
      attaaggcc atcctggca tcggccaac tcgatcatt 
      tcggcctgc aaaatgttc gtcccgaat ccgaacaac 
      tgtttcgaa ctgtacgga tttgatatt ttgattgat 
      gccaactta aagccgtgg cttttggaa gtgaatcta 
      tcgccttct ttgggctgc gacagcccg ctagacgtg 
      cgattgaaa tcggccatg ttgtctgat cttttaaca 
      cttgtggga atccccgca gttgacccg attctacgg 
      cccacaacc aacagtctc acatcggcc agtattaaa 
      tcaagtagt cgagtcaag cttggacat tgccggagg 
      gttcattcg gccgatggc ttgagcagt actttaccg 
      aggaaaaat tcttcgatc cacggcgat cggctatcg 
      tcgagtgct ttaacgcta acacccgaa gaaacacga 
      gcgattaaa ttggccaaa agtcagttt gaacgccga 
      ggcggcttt gtcaggatt ttcccggcc cttgacagt 
      tgggcgaaa tattcgcag tatttagat ccggccacc 
      ggtatcccg atctctggc aataacaac tcctacacg 
      agttataac ctatccggt gcccacaat tacaacttg 
      ttactttac acacatttg tttcccgat gcacctgtc 
      aatacaaaa ctgacggat aggtcaagt ctgaggtat 
      agttcgttg gaccggaaa cgcgacctc tcattggac 
      agccacaca ggctcgtca ttggcgcgt caagaccga 
      tacgagcgt cagttgagc caaggacat cgacaatca 
      cttgccccc caaaaacaa ctaaaagag gggcgttcg 
      attgagttg cgcaagcaa atcgttgac atgttgaga 
      aatggcaaa aaaatgacg caaagcgag actcggaag 
      actttcagt cattactta gtgtgtgtg ttacgccgg 
      atttcctca gggccggac caggaggac cacatggaa 
      atcgtgttg aagtttttg cagaaggcg tcaagttat 
      ctgcggacg ccttaccaa gtcaagacg cccagccag 
      agcctggat tacaaggat agggcggcc gtggttgcg 
      aaacaattg aacgatttc ctgtacttg tacaatcgg 
      gagacggag ctgtttgtt gataagacg gaaaagccg 
      agccagatt ccggccaaa ctgtatgcc gagtttttg 
      gaaaatgca agggagtgc gatctggag gagattttg 
      gtgcaccag acgacgcag acagtgcca acgcacggg 
      ggcaggacg gggttccag ggggtgctg aagtgcatc 
      cccaacgtg cccaagtcg tgcggcctg aagcccccg 
      tacaagaca acgacgaag ggcaagggc gtcaaggtg 
      gcgtccttg tcttga
    mRNA sequence
    • >TC009196 RA
      gacaattct tcctccgat ttgtacttg cgggcccag 
      tctgcattg ccttggtac acctggtaa aggacgaag 
      ccgaaggcg ttccacgcg gttaacatg tagaatatg 
      gaaaggcgc tgtttccac acgtgggcc cgctgctca 
      gggatctga ttcgtgtgt cttctgata aaattacgc 
      aaccaacgg gggttgaag agcgacatt ttggggtta 
      tgtaacacg caaaatcgg cgagaaata acacgtcgt 
      ggagtggtt tggggcgaa ctcttgacg cgtttagtg 
      tttttttgg gcgattcca gcggttcgt tctagtgat 
      aatcccggg tttttgcca taaaattag aggttagat 
      ggcagtttc gggccctac tttaccggc cgcgatttg 
      aaaaaataa acaaaatgg cgtcccttt aaaaagtat 
      caaaattcg aagtgaaat tttcgtgtc acagtcaat 
      taaaatact atgaataag taatttcgg cgcaacgga 
      cccaaattt tcagttacc cattacatt ggccaagat 
      ggaaaaaag tatcggaga caacggcga cgagatatc 
      aacgtcaag tgtgagcag ttcggagtc gatcaattt 
      cgcgtcaaa gttgcccca agctgaatt aaatcgtat 
      cgcgtccaa acctatcgt gttctcctg gcgcccccc 
      agacttccc cgaagactc cctcgacaa tgcctcgtc 
      ccagtgcat gctgttcga cagccccct ttcggaggc 
      ggaaaagag caaagtgat aacccaaga accgacagt 
      gccccccga cacgtggct cctcggtgg caccctcgg 
      caatcgtca cgcaactct agtttttaa aagttcagc 
      tctggcaaa ctgtactca aaaatcaat tcaagaaaa 
      accaggcca caaacttca catcacgta caaagtact 
      tcaagccga aacgaaact cctttcaaa gttattgga 
      atgtcatgg aatcgttga agttgcacc gaactcgtc 
      cgattttaa tcttttatg gacgggttc gcacccgaa 
      accgggcgc tttgcgatc gttagcccc ccatcagcg 
      ggtcaatca ttttccacg ctcttacga attgaccag 
      gaaagacag attgtataa aaatatcga gcggatgca 
      gcactgcaa aggctacaa acatttcga ttttattcc 
      gcaaacttt tgtaatgcc gaccgaatt tggggagtt 
      gtgctcgac gcatcatcg catcaaggg gccctggat 
      agtcaagcc agtggcgtc gagtcgggg gcgaggtat 
      ttttattgt cgaaacacc aaatcaagt gcctctgga 
      agagccggt cgttgtggc caagtatat atcgcgacc 
      gttgcttgt cgctggtca caaatgtga tttgcgatt 
      atacgtcgc tgttacgtc gtttgatcc tttgcttgt 
      ttatattta cgaagaagg tttagtcag atttgctac 
      cgtcaagta cgattctag tcataaaca gctgtggaa 
      tccctgtat gcatctgtg taattatag cataaataa 
      gtatcatag tgattatgt caaatcgga cgacccgag 
      cgccgaaaa cgtgggtca taagtggac actgagtgc 
      ccttttgcg acatttgaa agccgaggg gaaaaacac 
      ggcggtttt aatgtcaca ggtcgagga tttggtcat 
      taaggccat cctggcatc ggccaactc gatcatttc 
      ggcctgcaa aatgttcgt cccgaatcc gaacaactg 
      tttcgaact gtacggatt tgatatttt gattgatgc 
      caacttaaa gccgtggct tttggaagt gaatctatc 
      gccttcttt gggctgcga cagcccgct agacgtgcg 
      attgaaatc ggccatgtt gtctgatct tttaacact 
      tgtgggaat ccccgcagt tgacccgat tctacggcc 
      cacaaccaa cagtctcac atcggccag tattaaatc 
      aagtagtcg agtcaagct tggacattg ccggagggt 
      tcattcggc cgatggctt gagcagtac tttaccgag 
      gaaaaattc ttcgatcca cggcgatcg gctatcgtc 
      gagtgcttt aacgctaac acccgaaga aacacgagc 
      gattaaatt ggccaaaag tcagtttga acgccgagg 
      cggctttgt caggatttt cccggccct tgacagttg 
      ggcgaaata ttcgcagta tttagatcc ggccaccgg 
      tatcccgat ctctggcaa taacaactc ctacacgag 
      ttataacct atccggtgc ccacaatta caacttgtt 
      actttacac acatttgtt tcccgatgc acctgtcaa 
      tacaaaact gacggatag gtcaagtct gaggtatag 
      ttcgttgga ccggaaacg cgacctctc attggacag 
      ccacacagg ctcgtcatt ggcgcgtca agaccgata 
      cgagcgtca gttgagcca aggacatcg acaatcact 
      tgcccccca aaaacaact aaaagaggg gcgttcgat 
      tgagttgcg caagcaaat cgttgacat gttgagaaa 
      tggcaaaaa aatgacgca aagcgagac tcggaagac 
      tttcagtca ttacttagt gtgtgtgtt acgccggat 
      ttcctcagg gccggacca ggaggacca catggaaat 
      cgtgttgaa gtttttgca gaaggcgtc aagttatct 
      gcggacgcc ttaccaagt caagacgcc cagccagag 
      cctggatta caaggatag ggcggccgt ggttgcgaa 
      acaattgaa cgatttcct gtacttgta caatcggga 
      gacggagct gtttgttga taagacgga aaagccgag 
      ccagattcc ggccaaact gtatgccga gtttttgga 
      aaatgcaag ggagtgcga tctggagga gattttggt 
      gcaccagac gacgcagac agtgccaac gcacggggg 
      caggacggg gttccaggg ggtgctgaa gtgcatccc 
      caacgtgcc caagtcgtg cggcctgaa gcccccgta 
      caagacaac gacgaaggg caagggcgt caaggtggc 
      gtccttgtc ttgattgtt ttgcacttg ttcgtaatt 
      tgtcggaat aatactctt ggttgagac ttgttgatt 
      tattctagt ttttaaggg ttttggttc gtttgaatg 
      tttattttt ctattatta ccaaagttc attccaagc 
      cattttttt ttgattgag tacaatttt tcaacgaaa 
      ccagtgata atgaatgta ctttgctta caactaagc 
      tgctgccaa ataaatatt tttcatacg taaaatcac 
      gtgtagtga ctaggaatt tttacactc gtattgtga 
      tatgaatgg aagtgcttg agctatttt cgatacttg 
      ttacactgc tgtttgacg gtttttcta catttaaga 
      gtacttagg tcttgctca aatttttgc ctaaacttt 
      gttggtttt ttttctagt gactggacg ttaggtagg 
      aacagattt tgtacacta ctagtttta cactttgag 
      cacaagcaa tgttgttgt ggttggtcg ttgtttcgc 
      ttaataaaa ctctgaaat tacc