| General information | |||
| TC009191 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 3264211..3267354 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009191 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01493 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 10 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC009191 RA
MATYEEYIQ QNEDRDGIR FTWNVWPSS RIEATRLVV PLGCLYQPI KERQDLPPI QYDPVLCTR NNCRAILNP LCQVDYRAK LWVCNFCFQ RNPFPPQYA AISEQHQPA ELMPMFSTI EYTITRAQC LPPIYLLVV DTCMDEEEL GALKDSLQM SLSLLPPTA LIGLITFGK MVQVHELGT EGCSKSYVF RGTKDLTAK QIQEMLGIG KVAVAQPQR GAPQAPIPP ASRFLQPIS KCDMSLTDL IGELQRDPW PVGQGKRPL RSTGAALSI AIGLLECTY ANTGARVMM FLGGPCSQG PGQVVNDDL KQPIRSHHD IQKDNAKYM KKAIKHYEG LAMRAATNG HCVDIYSCA LDQTGLMEM KQCCNSTGG HMVMGDSFN SSLFKQTFQ RVFARDAKN ELKMAFNAT LEVKCSREL KVQGGIGSC VSLNVKSPL VSDTEIGMG NTVQWKMCT LTPSATIAL FFEVVNQHS APIPQGGRG CIQFITQYQ HSSGQRRIR VTTIARNWA DATANIHHI SAGFDQEAA AVLMARMAV YRAETDESP DVLRWVDRM LIRLCQKFG EYNKDDPNS FRLSENFSL YPQFMYHLR RSQFLQVFN NSPDETSFY RHMLMREDL TQSLIMIQP ILYSYSFNG PPEPVLLDT SSIQPDRIL LMDTFFQIL IFHGETIAQ WRNLKYQDM PEYENFRQL LQAPVDDAQ EILQTRFPM PRYIDTEQG GSQARFLLS KVNPSQTHN NMYAYGGAV PASADGGAP VLTDDVSLQ VFMDHLKKL AVSSTA
- >TC009191 RB
MATYEEYIQ QNEDRDGIR FTWNVWPSS RIEATRLVV PLGCLYQPI KERQDLPPI QYDPVLCTR NNCRAILNP LCQVDYRAK LWVCNFCFQ RNPFPPQYA AISEQHQPA ELMPMFSTI EYTITRAQC LPPIYLLVV DTCMDEEEL GALKDSLQM SLSLLPPTA LIGLITFGK MVQVHELGT EGCSKSYVF RGTKDLTAK QIQEMLGIG KVAVAQPQR GAPQAPIPP ASRFLQPIS KCDMSLTDL IGELQRDPW PVGQGKRPL RSTGAALSI AIGLLECTY ANTGARVMM FLGGPCSQG PGQVVNDDL KQPIRSHHD IQKDNAKYM KKAIKHYEG LAMRAATNG HCVDIYSCA LDQTGLMEM KQCCNSTGG HMVMGDSFN SSLFKQTFQ RVFARDAKN ELKMAFNAT LEVKCSREL KVQGGIGSC VSLNVKSPL VSDTEIGMG NTVQWKMCT LTPSATIAL FFEVVNQHS APIPQGGRG CIQFITQYQ HSSGQRRIR VTTIARNWA DATANIHHI SAGFDQEAA AVLMARMAV YRAETDESP DVLRWVDRM LIRLCQKFG EYNKDDPNS FRLSENFSL YPQFMYHLR RSQFLQVFN NSPDETSFY RHMLMREDL TQSLIMIQP ILYSYSFNG PPEPVLLDT SSIQPDRIL LMDTFFQIL IFHGETIAQ WRNLKYQDM PEYENFRQL LQAPVDDAQ EILQTRFPM PRYIDTEQG GSQARFLLS KVNPSQTHN NMYAYGGDG GAPVLTDDV SLQVFMDHL KKLAVSSTA
- >TC009191 RA
atggccacc tacgaagaa tacatccag cagaatgag gaccgcgac ggcatccgt ttcacttgg aacgtgtgg ccgtcgagt cgcatcgaa gccacccgt ttggtggtc cccctcggt tgtctctac caacccatc aaggagcgc caagacctg ccccccatc caatacgac cctgtccta tgtacccgt aacaactgt cgtgcgatt ctgaacccg ctgtgtcaa gtcgactac cgggcaaag ctgtgggtt tgcaatttc tgtttccaa cggaatccg tttccgcct caatatgcg gccatttcc gagcaacac caaccggcc gaactcatg ccaatgttc tccaccatt gagtacaca ataacacgc gcccaatgc ctgcccccc atctacctc ctagtcgtc gatacgtgc atggacgag gaggagctg ggggccctt aaagactcg cttcagatg tctttgagc cttctcccg ccgacggcc ctcataggg ctcatcaca ttcgggaaa atggtccaa gttcacgaa ctgggcact gagggctgt agcaaatcg tacgttttt cgggggacc aaagacctg acagctaaa caaatccag gagatgctc gggattggg aaagtggcc gtggcgcag ccccagcga ggggccccg caagccccc atcccgccc gccagtcgg ttcctccaa cccatctca aagtgcgat atgagtctg actgatttg atcggggag ctgcaaaga gacccctgg cctgtgggg caaggcaag cggcccttg agatcaaca ggggcggcc ttgtccata gcaataggg ctccttgag tgcacgtat gccaatacg ggggcccgg gttatgatg ttcctaggg gggccctgc tcgcaaggg ccgggtcag gtggttaac gacgatttg aaacaacca atcaggtcg catcatgat attcagaaa gataacgcc aagtatatg aagaaggcg atcaagcat tatgagggg ttggctatg agggctgcc actaatggg cattgtgtt gatatttat tcgtgtgct ttggaccaa acggggctg atggagatg aagcagtgt tgcaattcg acggggggg catatggtt atgggggac tcgttcaat tcgtcgctt tttaaacaa acgtttcag cgggttttt gcaagagat gccaagaat gagctgaaa atggccttc aatgccaca ctggaagtc aagtgttca agggaactc aaagtgcaa ggaggtatt ggctcttgt gtgtccctc aatgtgaaa agtcccctc gtttcggac actgaaatc ggaatgggt aacacagtc caatggaaa atgtgcact ttgacgccg agtgcaaca attgcccta tttttcgaa gtggtcaat caacattcg gcccccata ccgcaagga ggccgtgga tgcattcag ttcataaca caatatcaa cattcgagc ggccagagg cgcattcgc gttacaacc atcgcccga aattgggcc gatgccacg gccaacatc caccacatc agcgccggc tttgaccag gaagcggcg gctgtttta atggcccgc atggccgtt tatcgagct gaaaccgac gaaagcccc gatgtgttg cgatgggtc gaccgtatg ttaattcgt ctgtgccaa aaattcggc gaatacaac aaagacgac cctaacagt ttccgtttg agtgaaaac ttcagtctc tatccccaa ttcatgtac catttgcgc cgctcccaa ttcctccaa gttttcaac aactcccca gacgagacc tcgttctac cgccacatg ctgatgcgg gaggacctc acccaaagt ctcattatg atccagccg attttatac agttatagt ttcaacggc ccccctgaa cccgtcctc ctcgacact agttccatt caacccgat cgaatcctt ctcatggac acatttttc caaattttg attttccac ggtgagaca atcgcccaa tggaggaac ctcaagtac caggacatg ccagagtac gagaatttc cggcaattg ttacaagca ccggtggat gacgcccag gaaatcctc cagacgagg ttccccatg cctaggtac atcgacacg gaacaaggg ggctcccaa gccaggttt ttactatcg aaagtcaac ccgagccaa acacataac aatatgtac gcgtacgga ggggcggta cctgcgagt gcggacggc ggggccccg gttttgacc gacgacgtc tcgttgcaa gtgtttatg gatcatttg aagaaactg gcggtctct tcaacggct taa
- >TC009191 RB
atggccacc tacgaagaa tacatccag cagaatgag gaccgcgac ggcatccgt ttcacttgg aacgtgtgg ccgtcgagt cgcatcgaa gccacccgt ttggtggtc cccctcggt tgtctctac caacccatc aaggagcgc caagacctg ccccccatc caatacgac cctgtccta tgtacccgt aacaactgt cgtgcgatt ctgaacccg ctgtgtcaa gtcgactac cgggcaaag ctgtgggtt tgcaatttc tgtttccaa cggaatccg tttccgcct caatatgcg gccatttcc gagcaacac caaccggcc gaactcatg ccaatgttc tccaccatt gagtacaca ataacacgc gcccaatgc ctgcccccc atctacctc ctagtcgtc gatacgtgc atggacgag gaggagctg ggggccctt aaagactcg cttcagatg tctttgagc cttctcccg ccgacggcc ctcataggg ctcatcaca ttcgggaaa atggtccaa gttcacgaa ctgggcact gagggctgt agcaaatcg tacgttttt cgggggacc aaagacctg acagctaaa caaatccag gagatgctc gggattggg aaagtggcc gtggcgcag ccccagcga ggggccccg caagccccc atcccgccc gccagtcgg ttcctccaa cccatctca aagtgcgat atgagtctg actgatttg atcggggag ctgcaaaga gacccctgg cctgtgggg caaggcaag cggcccttg agatcaaca ggggcggcc ttgtccata gcaataggg ctccttgag tgcacgtat gccaatacg ggggcccgg gttatgatg ttcctaggg gggccctgc tcgcaaggg ccgggtcag gtggttaac gacgatttg aaacaacca atcaggtcg catcatgat attcagaaa gataacgcc aagtatatg aagaaggcg atcaagcat tatgagggg ttggctatg agggctgcc actaatggg cattgtgtt gatatttat tcgtgtgct ttggaccaa acggggctg atggagatg aagcagtgt tgcaattcg acggggggg catatggtt atgggggac tcgttcaat tcgtcgctt tttaaacaa acgtttcag cgggttttt gcaagagat gccaagaat gagctgaaa atggccttc aatgccaca ctggaagtc aagtgttca agggaactc aaagtgcaa ggaggtatt ggctcttgt gtgtccctc aatgtgaaa agtcccctc gtttcggac actgaaatc ggaatgggt aacacagtc caatggaaa atgtgcact ttgacgccg agtgcaaca attgcccta tttttcgaa gtggtcaat caacattcg gcccccata ccgcaagga ggccgtgga tgcattcag ttcataaca caatatcaa cattcgagc ggccagagg cgcattcgc gttacaacc atcgcccga aattgggcc gatgccacg gccaacatc caccacatc agcgccggc tttgaccag gaagcggcg gctgtttta atggcccgc atggccgtt tatcgagct gaaaccgac gaaagcccc gatgtgttg cgatgggtc gaccgtatg ttaattcgt ctgtgccaa aaattcggc gaatacaac aaagacgac cctaacagt ttccgtttg agtgaaaac ttcagtctc tatccccaa ttcatgtac catttgcgc cgctcccaa ttcctccaa gttttcaac aactcccca gacgagacc tcgttctac cgccacatg ctgatgcgg gaggacctc acccaaagt ctcattatg atccagccg attttatac agttatagt ttcaacggc ccccctgaa cccgtcctc ctcgacact agttccatt caacccgat cgaatcctt ctcatggac acatttttc caaattttg attttccac ggtgagaca atcgcccaa tggaggaac ctcaagtac caggacatg ccagagtac gagaatttc cggcaattg ttacaagca ccggtggat gacgcccag gaaatcctc cagacgagg ttccccatg cctaggtac atcgacacg gaacaaggg ggctcccaa gccaggttt ttactatcg aaagtcaac ccgagccaa acacataac aatatgtac gcgtacgga ggggacggc ggggccccg gttttgacc gacgacgtc tcgttgcaa gtgtttatg gatcatttg aagaaactg gcggtctct tcaacggct taa
- >TC009191 RA
tatgacgtg gatcttgaa atgtgtaac aaatgtaaa atgtttttt taaactttt aaaataaat taaaatcaa gcatttgac gcgtttttt gtgtttgtg accacttgg cataagccg ttctgcgat ggccaccta cgaagaata catccagca gaatgagga ccgcgacgg catccgttt cacttggaa cgtgtggcc gtcgagtcg catcgaagc cacccgttt ggtggtccc cctcggttg tctctacca acccatcaa ggagcgcca agacctgcc ccccatcca atacgaccc tgtcctatg tacccgtaa caactgtcg tgcgattct gaacccgct gtgtcaagt cgactaccg ggcaaagct gtgggtttg caatttctg tttccaacg gaatccgtt tccgcctca atatgcggc catttccga gcaacacca accggccga actcatgcc aatgttctc caccattga gtacacaat aacacgcgc ccaatgcct gccccccat ctacctcct agtcgtcga tacgtgcat ggacgagga ggagctggg ggcccttaa agactcgct tcagatgtc tttgagcct tctcccgcc gacggccct catagggct catcacatt cgggaaaat ggtccaagt tcacgaact gggcactga gggctgtag caaatcgta cgtttttcg ggggaccaa agacctgac agctaaaca aatccagga gatgctcgg gattgggaa agtggccgt ggcgcagcc ccagcgagg ggccccgca agcccccat cccgcccgc cagtcggtt cctccaacc catctcaaa gtgcgatat gagtctgac tgatttgat cggggagct gcaaagaga cccctggcc tgtggggca aggcaagcg gcccttgag atcaacagg ggcggcctt gtccatagc aatagggct ccttgagtg cacgtatgc caatacggg ggcccgggt tatgatgtt cctaggggg gccctgctc gcaagggcc gggtcaggt ggttaacga cgatttgaa acaaccaat caggtcgca tcatgatat tcagaaaga taacgccaa gtatatgaa gaaggcgat caagcatta tgaggggtt ggctatgag ggctgccac taatgggca ttgtgttga tatttattc gtgtgcttt ggaccaaac ggggctgat ggagatgaa gcagtgttg caattcgac gggggggca tatggttat gggggactc gttcaattc gtcgctttt taaacaaac gtttcagcg ggtttttgc aagagatgc caagaatga gctgaaaat ggccttcaa tgccacact ggaagtcaa gtgttcaag ggaactcaa agtgcaagg aggtattgg ctcttgtgt gtccctcaa tgtgaaaag tcccctcgt ttcggacac tgaaatcgg aatgggtaa cacagtcca atggaaaat gtgcacttt gacgccgag tgcaacaat tgccctatt tttcgaagt ggtcaatca acattcggc ccccatacc gcaaggagg ccgtggatg cattcagtt cataacaca atatcaaca ttcgagcgg ccagaggcg cattcgcgt tacaaccat cgcccgaaa ttgggccga tgccacggc caacatcca ccacatcag cgccggctt tgaccagga agcggcggc tgttttaat ggcccgcat ggccgttta tcgagctga aaccgacga aagccccga tgtgttgcg atgggtcga ccgtatgtt aattcgtct gtgccaaaa attcggcga atacaacaa agacgaccc taacagttt ccgtttgag tgaaaactt cagtctcta tccccaatt catgtacca tttgcgccg ctcccaatt cctccaagt tttcaacaa ctccccaga cgagacctc gttctaccg ccacatgct gatgcggga ggacctcac ccaaagtct cattatgat ccagccgat tttatacag ttatagttt caacggccc ccctgaacc cgtcctcct cgacactag ttccattca acccgatcg aatccttct catggacac atttttcca aattttgat tttccacgg tgagacaat cgcccaatg gaggaacct caagtacca ggacatgcc agagtacga gaatttccg gcaattgtt acaagcacc ggtggatga cgcccagga aatcctcca gacgaggtt ccccatgcc taggtacat cgacacgga acaaggggg ctcccaagc caggttttt actatcgaa agtcaaccc gagccaaac acataacaa tatgtacgc gtacggagg ggcggtacc tgcgagtgc ggacggcgg ggccccggt tttgaccga cgacgtctc gttgcaagt gtttatgga tcatttgaa gaaactggc ggtctcttc aacggctta aactacttt attcttatt tattaagta caatagtaa caagaaaat atctctggt taa
- >TC009191 RB
tatgacgtg gatcttgaa atgtgtaac aaatgtaaa atgtttttt taaactttt aaaataaat taaaatcaa gcatttgac gcgtttttt gtgtttgtg accacttgg cataagccg ttctgcgat ggccaccta cgaagaata catccagca gaatgagga ccgcgacgg catccgttt cacttggaa cgtgtggcc gtcgagtcg catcgaagc cacccgttt ggtggtccc cctcggttg tctctacca acccatcaa ggagcgcca agacctgcc ccccatcca atacgaccc tgtcctatg tacccgtaa caactgtcg tgcgattct gaacccgct gtgtcaagt cgactaccg ggcaaagct gtgggtttg caatttctg tttccaacg gaatccgtt tccgcctca atatgcggc catttccga gcaacacca accggccga actcatgcc aatgttctc caccattga gtacacaat aacacgcgc ccaatgcct gccccccat ctacctcct agtcgtcga tacgtgcat ggacgagga ggagctggg ggcccttaa agactcgct tcagatgtc tttgagcct tctcccgcc gacggccct catagggct catcacatt cgggaaaat ggtccaagt tcacgaact gggcactga gggctgtag caaatcgta cgtttttcg ggggaccaa agacctgac agctaaaca aatccagga gatgctcgg gattgggaa agtggccgt ggcgcagcc ccagcgagg ggccccgca agcccccat cccgcccgc cagtcggtt cctccaacc catctcaaa gtgcgatat gagtctgac tgatttgat cggggagct gcaaagaga cccctggcc tgtggggca aggcaagcg gcccttgag atcaacagg ggcggcctt gtccatagc aatagggct ccttgagtg cacgtatgc caatacggg ggcccgggt tatgatgtt cctaggggg gccctgctc gcaagggcc gggtcaggt ggttaacga cgatttgaa acaaccaat caggtcgca tcatgatat tcagaaaga taacgccaa gtatatgaa gaaggcgat caagcatta tgaggggtt ggctatgag ggctgccac taatgggca ttgtgttga tatttattc gtgtgcttt ggaccaaac ggggctgat ggagatgaa gcagtgttg caattcgac gggggggca tatggttat gggggactc gttcaattc gtcgctttt taaacaaac gtttcagcg ggtttttgc aagagatgc caagaatga gctgaaaat ggccttcaa tgccacact ggaagtcaa gtgttcaag ggaactcaa agtgcaagg aggtattgg ctcttgtgt gtccctcaa tgtgaaaag tcccctcgt ttcggacac tgaaatcgg aatgggtaa cacagtcca atggaaaat gtgcacttt gacgccgag tgcaacaat tgccctatt tttcgaagt ggtcaatca acattcggc ccccatacc gcaaggagg ccgtggatg cattcagtt cataacaca atatcaaca ttcgagcgg ccagaggcg cattcgcgt tacaaccat cgcccgaaa ttgggccga tgccacggc caacatcca ccacatcag cgccggctt tgaccagga agcggcggc tgttttaat ggcccgcat ggccgttta tcgagctga aaccgacga aagccccga tgtgttgcg atgggtcga ccgtatgtt aattcgtct gtgccaaaa attcggcga atacaacaa agacgaccc taacagttt ccgtttgag tgaaaactt cagtctcta tccccaatt catgtacca tttgcgccg ctcccaatt cctccaagt tttcaacaa ctccccaga cgagacctc gttctaccg ccacatgct gatgcggga ggacctcac ccaaagtct cattatgat ccagccgat tttatacag ttatagttt caacggccc ccctgaacc cgtcctcct cgacactag ttccattca acccgatcg aatccttct catggacac atttttcca aattttgat tttccacgg tgagacaat cgcccaatg gaggaacct caagtacca ggacatgcc agagtacga gaatttccg gcaattgtt acaagcacc ggtggatga cgcccagga aatcctcca gacgaggtt ccccatgcc taggtacat cgacacgga acaaggggg ctcccaagc caggttttt actatcgaa agtcaaccc gagccaaac acataacaa tatgtacgc gtacggagg ggacggcgg ggccccggt tttgaccga cgacgtctc gttgcaagt gtttatgga tcatttgaa gaaactggc ggtctcttc aacggctta aactacttt attcttatt tattaagta caatagtaa caagaaaat atctctggt taa
