Status
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Gene information
General information
TC009183 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3337388..3341614
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009183
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01492

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 1
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009183 RA
      MAQAGSKKS QLTKINSKT GINSLIIFV VLLLAWIAG 
      FSSRLFAVI RFESIIHEF DPWFNYRAT AYMVKHGFY 
      NFLNWFDER AWYPLGRIV GGTVYPGLM ITSGSIHYV 
      LHLLNINVH IRDICVFLA PIFSGLTAI STYFLTKEL 
      WSAGAGLFA ACFIAIVPG YISRSVAGS YDNEGIAIF 
      ALQFTYFLW IKSVKTGSV FWAVAAALS YFYMVSAWG 
      GYVFIINLI PLHVFVLLL MGRFSNRLF TSYTTFYIL 
      GLICSMQIP FVGFQPIRT SEHMAASGV FALLFAVAT 
      LKYLQTMLS KSEFKSLVI FGGLISAGV VFLTVVGLT 
      YAGYIAPWS GRFYSLWDT GYAKIHIPI IASVSEHQP 
      TTWFSFFFD LHILVATFP AGLWYCIKN VNDERVFVI 
      LYALSAVYF AGVMVRLML TLTPVVCIL SGIAFSGLL 
      ELFLREDDT PRNEDSDDD EQSPPNNRR LYDKAVAGR 
      VPKMKHEQN KESDGIGPN IRSIVTVVI MLLLMLFAV 
      HCTWVTSNA YSSPSIVLA SYGSDGSRQ ILDDFREAY 
      FWLSQNTAD EARVMSWWD YGYQIAGMA NRTTLVDNN 
      TWNNSHIAL VGKAMSSNE SAAYEIMTA LDVDYVLVI 
      FGGVIGYSG DDINKFLWM VRIAEGEHP KDIRESDYF 
      TEHGEFRVD AEGAPTLLN SLMYKLSYY RFGELKLDY 
      RTPAGYDRT RNAIIGNKD FQLTYLEEA YTTEHWLVR 
      VYRVKKPDE FNRPRIPVA NRVVKSQNF VSKKSTKRK 
      KGTIKNKPV IVKGKRLSK QA
    CDS sequence
    • >TC009183 RA
      atggctcaa gccggttcg aaaaagtcc caattaacc 
      aaaataaat agtaaaacc ggaataaac agtcttata 
      attttcgtc gttctcctc ctggcatgg atcgccggc 
      ttttcgtcc cgtctcttc gccgtgatc cgtttcgaa 
      agcataatt cacgaattt gatccatgg ttcaattac 
      cgcgccacg gcgtacatg gtcaaacac ggcttttac 
      aattttttg aactggttt gatgagcgc gcctggtac 
      cccttgggg cgcatcgtg gggggcacg gtgtacccc 
      ggattgatg atcacgtca gggagcata cactacgtg 
      ttgcattta ttgaacatc aatgtacat attagggat 
      atttgtgtg ttcctggcc cccatcttc agcggcctc 
      acggccatc tcgacctac tttctgacc aaggagctg 
      tggtcggcg ggggccggc cttttcgcg gcgtgtttt 
      attgccatt gtgcctgga tacatcagt cgttctgtt 
      gccggaagt tacgataat gaaggtatc gcaattttc 
      gcacttcaa ttcacttat ttcctctgg attaagtcg 
      gtgaagacg gggtcggtg ttttgggcc gtcgcagcg 
      gcactttcg tacttttat atggtctcc gcatgggga 
      ggctacgtg ttcatcatc aatctaatc ccgcttcat 
      gtgtttgtc ctccttctg atgggacga ttttcgaac 
      cgattattt accagttac accactttc tacatactt 
      gggttgatt tgctccatg cagatacca tttgttggg 
      ttccaaccc attcgtacc agcgaacac atggcggct 
      tctggtgtt tttgcatta cttttcgcc gttgcgacg 
      ctaaaatac ctacaaacg atgctctca aagtctgag 
      tttaagtcg ttggtgatt tttggtggt ttaatatcg 
      gcaggtgtt gtttttcta acagtggta ggactgacc 
      tatgctggg tatattgca ccatggagt ggccggttt 
      tattcattg tgggatact ggatatgct aaaattcat 
      attccaata attgcgtcg gtttctgaa catcaaccg 
      accacttgg ttctcgttt ttctttgat ttgcatatt 
      ttagttgca acttttccc gctggtctg tggtactgt 
      atcaaaaat gtgaacgac gagagggtt ttcgtgatt 
      ctgtacgcc ctgagtgcg gtttatttc gccggcgtt 
      atggtccgt ctcatgtta accctaaca cctgtggtg 
      tgcattttg agcggaata gcattttcc ggtcttttg 
      gagctattt ttacgcgaa gatgacact ccacgtaac 
      gaagatagt gacgatgat gaacaatca cctccaaat 
      aatcgtcga ttatatgac aaggccgtt gccggccgt 
      gtgccaaaa atgaaacac gagcagaac aaagaatcg 
      gacggaatc ggtccaaat atacgtagc attgttacc 
      gttgtcata atgttattg ttgatgttg tttgcggta 
      cattgtact tgggtcact agcaatgct tattcaagc 
      ccaagtatc gtcctagca tcatacgga agtgacggt 
      tcccgtcag attttggac gattttcgc gaagcttac 
      ttttggttg tcgcaaaat acagccgac gaagcgaga 
      gtgatgagt tggtgggat tacgggtat cagatcgca 
      ggaatggcc aatcggacg acgttagtt gacaataac 
      acgtggaac aacagtcac atcgccctg gtggggaag 
      gcaatgtcg tccaatgag agcgcagct tacgagatc 
      atgaccgcc cttgatgtg gactacgtc ctagtgatc 
      ttcggcggt gtgattggc tattcgggc gatgatatt 
      aacaagttt ttgtggatg gtgcggatc gcagaaggc 
      gaacatccc aaagatata agagagagt gattatttt 
      acggaacat ggcgagttt agagtggat gctgaaggg 
      gcgccaact ctgttaaat tcactcatg tataagttg 
      agttattat cgatttggt gaattgaag ttggattat 
      cgtacaccg gctgggtat gatagaacc aggaacgca 
      attattggt aataaagat ttccaactt acctacctg 
      gaggaggcg tatacgact gagcattgg ctcgtgagg 
      gtatacagg gtgaagaaa ccagacgag tttaatagg 
      ccgaggatt cccgtggcg aatcgggtg gtcaaaagc 
      cagaatttc gtatcgaaa aagagcacc aaacgcaaa 
      aagggtaca attaaaaac aagccagtc atcgtgaag 
      ggcaaaaga ctgtcaaaa caggcgtaa
    mRNA sequence
    • >TC009183 RA
      ctcatcaag taacctctg agtgtgtgt atgcgtgtc 
      atgttttta cctgtcaaa aatggctca agccggttc 
      gaaaaagtc ccaattaac caaaataaa tagtaaaac 
      cggaataaa cagtcttat aattttcgt cgttctcct 
      cctggcatg gatcgccgg cttttcgtc ccgtctctt 
      cgccgtgat ccgtttcga aagcataat tcacgaatt 
      tgatccatg gttcaatta ccgcgccac ggcgtacat 
      ggtcaaaca cggctttta caatttttt gaactggtt 
      tgatgagcg cgcctggta ccccttggg gcgcatcgt 
      ggggggcac ggtgtaccc cggattgat gatcacgtc 
      agggagcat acactacgt gttgcattt attgaacat 
      caatgtaca tattaggga tatttgtgt gttcctggc 
      ccccatctt cagcggcct cacggccat ctcgaccta 
      ctttctgac caaggagct gtggtcggc gggggccgg 
      ccttttcgc ggcgtgttt tattgccat tgtgcctgg 
      atacatcag tcgttctgt tgccggaag ttacgataa 
      tgaaggtat cgcaatttt cgcacttca attcactta 
      tttcctctg gattaagtc ggtgaagac ggggtcggt 
      gttttgggc cgtcgcagc ggcactttc gtactttta 
      tatggtctc cgcatgggg aggctacgt gttcatcat 
      caatctaat cccgcttca tgtgtttgt cctccttct 
      gatgggacg attttcgaa ccgattatt taccagtta 
      caccacttt ctacatact tgggttgat ttgctccat 
      gcagatacc atttgttgg gttccaacc cattcgtac 
      cagcgaaca catggcggc ttctggtgt ttttgcatt 
      acttttcgc cgttgcgac gctaaaata cctacaaac 
      gatgctctc aaagtctga gtttaagtc gttggtgat 
      ttttggtgg tttaatatc ggcaggtgt tgtttttct 
      aacagtggt aggactgac ctatgctgg gtatattgc 
      accatggag tggccggtt ttattcatt gtgggatac 
      tggatatgc taaaattca tattccaat aattgcgtc 
      ggtttctga acatcaacc gaccacttg gttctcgtt 
      tttctttga tttgcatat tttagttgc aacttttcc 
      cgctggtct gtggtactg tatcaaaaa tgtgaacga 
      cgagagggt tttcgtgat tctgtacgc cctgagtgc 
      ggtttattt cgccggcgt tatggtccg tctcatgtt 
      aaccctaac acctgtggt gtgcatttt gagcggaat 
      agcattttc cggtctttt ggagctatt tttacgcga 
      agatgacac tccacgtaa cgaagatag tgacgatga 
      tgaacaatc acctccaaa taatcgtcg attatatga 
      caaggccgt tgccggccg tgtgccaaa aatgaaaca 
      cgagcagaa caaagaatc ggacggaat cggtccaaa 
      tatacgtag cattgttac cgttgtcat aatgttatt 
      gttgatgtt gtttgcggt acattgtac ttgggtcac 
      tagcaatgc ttattcaag cccaagtat cgtcctagc 
      atcatacgg aagtgacgg ttcccgtca gattttgga 
      cgattttcg cgaagctta cttttggtt gtcgcaaaa 
      tacagccga cgaagcgag agtgatgag ttggtggga 
      ttacgggta tcagatcgc aggaatggc caatcggac 
      gacgttagt tgacaataa cacgtggaa caacagtca 
      catcgccct ggtggggaa ggcaatgtc gtccaatga 
      gagcgcagc ttacgagat catgaccgc ccttgatgt 
      ggactacgt cctagtgat cttcggcgg tgtgattgg 
      ctattcggg cgatgatat taacaagtt tttgtggat 
      ggtgcggat cgcagaagg cgaacatcc caaagatat 
      aagagagag tgattattt tacggaaca tggcgagtt 
      tagagtgga tgctgaagg ggcgccaac tctgttaaa 
      ttcactcat gtataagtt gagttatta tcgatttgg 
      tgaattgaa gttggatta tcgtacacc ggctgggta 
      tgatagaac caggaacgc aattattgg taataaaga 
      tttccaact tacctacct ggaggaggc gtatacgac 
      tgagcattg gctcgtgag ggtatacag ggtgaagaa 
      accagacga gtttaatag gccgaggat tcccgtggc 
      gaatcgggt ggtcaaaag ccagaattt cgtatcgaa 
      aaagagcac caaacgcaa aaagggtac aattaaaaa 
      caagccagt catcgtgaa gggcaaaag actgtcaaa 
      acaggcgta agtagttgt tgttagttg tttggaacg 
      aagagtttt gttgattta gttctgcca gaaaagaag 
      gtttttcac ttgtgataa ctgtgtaaa ataaaggta 
      ataaatctt tttgcactg taaagtgtt ttgttcgac 
      attaaacat cactttgtg aaattggtt gtatttttt 
      tattaacat ttgtttatc ctagtatgt acagttgag 
      tttgttaca taaacaact taagaaact acacgtaca 
      cttagtaaa tggttgtag cggttacaa atcggtttt 
      ttgtttgtt tgaaacgaa aaggccttt gtcaacctt 
      attgcggcg tggaagatt cctcaatac aaaatataa 
      atccaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY