| General information | |||
| TC009183 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 3337388..3341614 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009183 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01492 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
ovary without detectable defects - Number of animals showing the phenotype: 1
fertility after larval injection decreased (no living offspring) -
- >TC009183 RA
MAQAGSKKS QLTKINSKT GINSLIIFV VLLLAWIAG FSSRLFAVI RFESIIHEF DPWFNYRAT AYMVKHGFY NFLNWFDER AWYPLGRIV GGTVYPGLM ITSGSIHYV LHLLNINVH IRDICVFLA PIFSGLTAI STYFLTKEL WSAGAGLFA ACFIAIVPG YISRSVAGS YDNEGIAIF ALQFTYFLW IKSVKTGSV FWAVAAALS YFYMVSAWG GYVFIINLI PLHVFVLLL MGRFSNRLF TSYTTFYIL GLICSMQIP FVGFQPIRT SEHMAASGV FALLFAVAT LKYLQTMLS KSEFKSLVI FGGLISAGV VFLTVVGLT YAGYIAPWS GRFYSLWDT GYAKIHIPI IASVSEHQP TTWFSFFFD LHILVATFP AGLWYCIKN VNDERVFVI LYALSAVYF AGVMVRLML TLTPVVCIL SGIAFSGLL ELFLREDDT PRNEDSDDD EQSPPNNRR LYDKAVAGR VPKMKHEQN KESDGIGPN IRSIVTVVI MLLLMLFAV HCTWVTSNA YSSPSIVLA SYGSDGSRQ ILDDFREAY FWLSQNTAD EARVMSWWD YGYQIAGMA NRTTLVDNN TWNNSHIAL VGKAMSSNE SAAYEIMTA LDVDYVLVI FGGVIGYSG DDINKFLWM VRIAEGEHP KDIRESDYF TEHGEFRVD AEGAPTLLN SLMYKLSYY RFGELKLDY RTPAGYDRT RNAIIGNKD FQLTYLEEA YTTEHWLVR VYRVKKPDE FNRPRIPVA NRVVKSQNF VSKKSTKRK KGTIKNKPV IVKGKRLSK QA
- >TC009183 RA
atggctcaa gccggttcg aaaaagtcc caattaacc aaaataaat agtaaaacc ggaataaac agtcttata attttcgtc gttctcctc ctggcatgg atcgccggc ttttcgtcc cgtctcttc gccgtgatc cgtttcgaa agcataatt cacgaattt gatccatgg ttcaattac cgcgccacg gcgtacatg gtcaaacac ggcttttac aattttttg aactggttt gatgagcgc gcctggtac cccttgggg cgcatcgtg gggggcacg gtgtacccc ggattgatg atcacgtca gggagcata cactacgtg ttgcattta ttgaacatc aatgtacat attagggat atttgtgtg ttcctggcc cccatcttc agcggcctc acggccatc tcgacctac tttctgacc aaggagctg tggtcggcg ggggccggc cttttcgcg gcgtgtttt attgccatt gtgcctgga tacatcagt cgttctgtt gccggaagt tacgataat gaaggtatc gcaattttc gcacttcaa ttcacttat ttcctctgg attaagtcg gtgaagacg gggtcggtg ttttgggcc gtcgcagcg gcactttcg tacttttat atggtctcc gcatgggga ggctacgtg ttcatcatc aatctaatc ccgcttcat gtgtttgtc ctccttctg atgggacga ttttcgaac cgattattt accagttac accactttc tacatactt gggttgatt tgctccatg cagatacca tttgttggg ttccaaccc attcgtacc agcgaacac atggcggct tctggtgtt tttgcatta cttttcgcc gttgcgacg ctaaaatac ctacaaacg atgctctca aagtctgag tttaagtcg ttggtgatt tttggtggt ttaatatcg gcaggtgtt gtttttcta acagtggta ggactgacc tatgctggg tatattgca ccatggagt ggccggttt tattcattg tgggatact ggatatgct aaaattcat attccaata attgcgtcg gtttctgaa catcaaccg accacttgg ttctcgttt ttctttgat ttgcatatt ttagttgca acttttccc gctggtctg tggtactgt atcaaaaat gtgaacgac gagagggtt ttcgtgatt ctgtacgcc ctgagtgcg gtttatttc gccggcgtt atggtccgt ctcatgtta accctaaca cctgtggtg tgcattttg agcggaata gcattttcc ggtcttttg gagctattt ttacgcgaa gatgacact ccacgtaac gaagatagt gacgatgat gaacaatca cctccaaat aatcgtcga ttatatgac aaggccgtt gccggccgt gtgccaaaa atgaaacac gagcagaac aaagaatcg gacggaatc ggtccaaat atacgtagc attgttacc gttgtcata atgttattg ttgatgttg tttgcggta cattgtact tgggtcact agcaatgct tattcaagc ccaagtatc gtcctagca tcatacgga agtgacggt tcccgtcag attttggac gattttcgc gaagcttac ttttggttg tcgcaaaat acagccgac gaagcgaga gtgatgagt tggtgggat tacgggtat cagatcgca ggaatggcc aatcggacg acgttagtt gacaataac acgtggaac aacagtcac atcgccctg gtggggaag gcaatgtcg tccaatgag agcgcagct tacgagatc atgaccgcc cttgatgtg gactacgtc ctagtgatc ttcggcggt gtgattggc tattcgggc gatgatatt aacaagttt ttgtggatg gtgcggatc gcagaaggc gaacatccc aaagatata agagagagt gattatttt acggaacat ggcgagttt agagtggat gctgaaggg gcgccaact ctgttaaat tcactcatg tataagttg agttattat cgatttggt gaattgaag ttggattat cgtacaccg gctgggtat gatagaacc aggaacgca attattggt aataaagat ttccaactt acctacctg gaggaggcg tatacgact gagcattgg ctcgtgagg gtatacagg gtgaagaaa ccagacgag tttaatagg ccgaggatt cccgtggcg aatcgggtg gtcaaaagc cagaatttc gtatcgaaa aagagcacc aaacgcaaa aagggtaca attaaaaac aagccagtc atcgtgaag ggcaaaaga ctgtcaaaa caggcgtaa
- >TC009183 RA
ctcatcaag taacctctg agtgtgtgt atgcgtgtc atgttttta cctgtcaaa aatggctca agccggttc gaaaaagtc ccaattaac caaaataaa tagtaaaac cggaataaa cagtcttat aattttcgt cgttctcct cctggcatg gatcgccgg cttttcgtc ccgtctctt cgccgtgat ccgtttcga aagcataat tcacgaatt tgatccatg gttcaatta ccgcgccac ggcgtacat ggtcaaaca cggctttta caatttttt gaactggtt tgatgagcg cgcctggta ccccttggg gcgcatcgt ggggggcac ggtgtaccc cggattgat gatcacgtc agggagcat acactacgt gttgcattt attgaacat caatgtaca tattaggga tatttgtgt gttcctggc ccccatctt cagcggcct cacggccat ctcgaccta ctttctgac caaggagct gtggtcggc gggggccgg ccttttcgc ggcgtgttt tattgccat tgtgcctgg atacatcag tcgttctgt tgccggaag ttacgataa tgaaggtat cgcaatttt cgcacttca attcactta tttcctctg gattaagtc ggtgaagac ggggtcggt gttttgggc cgtcgcagc ggcactttc gtactttta tatggtctc cgcatgggg aggctacgt gttcatcat caatctaat cccgcttca tgtgtttgt cctccttct gatgggacg attttcgaa ccgattatt taccagtta caccacttt ctacatact tgggttgat ttgctccat gcagatacc atttgttgg gttccaacc cattcgtac cagcgaaca catggcggc ttctggtgt ttttgcatt acttttcgc cgttgcgac gctaaaata cctacaaac gatgctctc aaagtctga gtttaagtc gttggtgat ttttggtgg tttaatatc ggcaggtgt tgtttttct aacagtggt aggactgac ctatgctgg gtatattgc accatggag tggccggtt ttattcatt gtgggatac tggatatgc taaaattca tattccaat aattgcgtc ggtttctga acatcaacc gaccacttg gttctcgtt tttctttga tttgcatat tttagttgc aacttttcc cgctggtct gtggtactg tatcaaaaa tgtgaacga cgagagggt tttcgtgat tctgtacgc cctgagtgc ggtttattt cgccggcgt tatggtccg tctcatgtt aaccctaac acctgtggt gtgcatttt gagcggaat agcattttc cggtctttt ggagctatt tttacgcga agatgacac tccacgtaa cgaagatag tgacgatga tgaacaatc acctccaaa taatcgtcg attatatga caaggccgt tgccggccg tgtgccaaa aatgaaaca cgagcagaa caaagaatc ggacggaat cggtccaaa tatacgtag cattgttac cgttgtcat aatgttatt gttgatgtt gtttgcggt acattgtac ttgggtcac tagcaatgc ttattcaag cccaagtat cgtcctagc atcatacgg aagtgacgg ttcccgtca gattttgga cgattttcg cgaagctta cttttggtt gtcgcaaaa tacagccga cgaagcgag agtgatgag ttggtggga ttacgggta tcagatcgc aggaatggc caatcggac gacgttagt tgacaataa cacgtggaa caacagtca catcgccct ggtggggaa ggcaatgtc gtccaatga gagcgcagc ttacgagat catgaccgc ccttgatgt ggactacgt cctagtgat cttcggcgg tgtgattgg ctattcggg cgatgatat taacaagtt tttgtggat ggtgcggat cgcagaagg cgaacatcc caaagatat aagagagag tgattattt tacggaaca tggcgagtt tagagtgga tgctgaagg ggcgccaac tctgttaaa ttcactcat gtataagtt gagttatta tcgatttgg tgaattgaa gttggatta tcgtacacc ggctgggta tgatagaac caggaacgc aattattgg taataaaga tttccaact tacctacct ggaggaggc gtatacgac tgagcattg gctcgtgag ggtatacag ggtgaagaa accagacga gtttaatag gccgaggat tcccgtggc gaatcgggt ggtcaaaag ccagaattt cgtatcgaa aaagagcac caaacgcaa aaagggtac aattaaaaa caagccagt catcgtgaa gggcaaaag actgtcaaa acaggcgta agtagttgt tgttagttg tttggaacg aagagtttt gttgattta gttctgcca gaaaagaag gtttttcac ttgtgataa ctgtgtaaa ataaaggta ataaatctt tttgcactg taaagtgtt ttgttcgac attaaacat cactttgtg aaattggtt gtatttttt tattaacat ttgtttatc ctagtatgt acagttgag tttgttaca taaacaact taagaaact acacgtaca cttagtaaa tggttgtag cggttacaa atcggtttt ttgtttgtt tgaaacgaa aaggccttt gtcaacctt attgcggcg tggaagatt cctcaatac aaaatataa atccaa
