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Gene information
General information
TC009183 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3337388..3341614
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009183
    iBeetle screen iB_01492

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection

    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 1
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009183 RA
      MAQAGSKKS QLTKINSKT GINSLIIFV VLLLAWIAG 
FSSRLFAVI RFESIIHEF DPWFNYRAT AYMVKHGFY 
NFLNWFDER AWYPLGRIV GGTVYPGLM ITSGSIHYV 
LHLLNINVH IRDICVFLA PIFSGLTAI STYFLTKEL 
WSAGAGLFA ACFIAIVPG YISRSVAGS YDNEGIAIF 
ALQFTYFLW IKSVKTGSV FWAVAAALS YFYMVSAWG 
GYVFIINLI PLHVFVLLL MGRFSNRLF TSYTTFYIL 
GLICSMQIP FVGFQPIRT SEHMAASGV FALLFAVAT 
LKYLQTMLS KSEFKSLVI FGGLISAGV VFLTVVGLT 
YAGYIAPWS GRFYSLWDT GYAKIHIPI IASVSEHQP 
TTWFSFFFD LHILVATFP AGLWYCIKN VNDERVFVI 
LYALSAVYF AGVMVRLML TLTPVVCIL SGIAFSGLL 
ELFLREDDT PRNEDSDDD EQSPPNNRR LYDKAVAGR 
VPKMKHEQN KESDGIGPN IRSIVTVVI MLLLMLFAV 
HCTWVTSNA YSSPSIVLA SYGSDGSRQ ILDDFREAY 
FWLSQNTAD EARVMSWWD YGYQIAGMA NRTTLVDNN 
TWNNSHIAL VGKAMSSNE SAAYEIMTA LDVDYVLVI 
FGGVIGYSG DDINKFLWM VRIAEGEHP KDIRESDYF 
TEHGEFRVD AEGAPTLLN SLMYKLSYY RFGELKLDY 
RTPAGYDRT RNAIIGNKD FQLTYLEEA YTTEHWLVR 
VYRVKKPDE FNRPRIPVA NRVVKSQNF VSKKSTKRK 
KGTIKNKPV IVKGKRLSK QA
    CDS sequence
    • >TC009183 RA
      atggctcaa gccggttcg aaaaagtcc caattaacc 
aaaataaat agtaaaacc ggaataaac agtcttata 
attttcgtc gttctcctc ctggcatgg atcgccggc 
ttttcgtcc cgtctcttc gccgtgatc cgtttcgaa 
agcataatt cacgaattt gatccatgg ttcaattac 
cgcgccacg gcgtacatg gtcaaacac ggcttttac 
aattttttg aactggttt gatgagcgc gcctggtac 
cccttgggg cgcatcgtg gggggcacg gtgtacccc 
ggattgatg atcacgtca gggagcata cactacgtg 
ttgcattta ttgaacatc aatgtacat attagggat 
atttgtgtg ttcctggcc cccatcttc agcggcctc 
acggccatc tcgacctac tttctgacc aaggagctg 
tggtcggcg ggggccggc cttttcgcg gcgtgtttt 
attgccatt gtgcctgga tacatcagt cgttctgtt 
gccggaagt tacgataat gaaggtatc gcaattttc 
gcacttcaa ttcacttat ttcctctgg attaagtcg 
gtgaagacg gggtcggtg ttttgggcc gtcgcagcg 
gcactttcg tacttttat atggtctcc gcatgggga 
ggctacgtg ttcatcatc aatctaatc ccgcttcat 
gtgtttgtc ctccttctg atgggacga ttttcgaac 
cgattattt accagttac accactttc tacatactt 
gggttgatt tgctccatg cagatacca tttgttggg 
ttccaaccc attcgtacc agcgaacac atggcggct 
tctggtgtt tttgcatta cttttcgcc gttgcgacg 
ctaaaatac ctacaaacg atgctctca aagtctgag 
tttaagtcg ttggtgatt tttggtggt ttaatatcg 
gcaggtgtt gtttttcta acagtggta ggactgacc 
tatgctggg tatattgca ccatggagt ggccggttt 
tattcattg tgggatact ggatatgct aaaattcat 
attccaata attgcgtcg gtttctgaa catcaaccg 
accacttgg ttctcgttt ttctttgat ttgcatatt 
ttagttgca acttttccc gctggtctg tggtactgt 
atcaaaaat gtgaacgac gagagggtt ttcgtgatt 
ctgtacgcc ctgagtgcg gtttatttc gccggcgtt 
atggtccgt ctcatgtta accctaaca cctgtggtg 
tgcattttg agcggaata gcattttcc ggtcttttg 
gagctattt ttacgcgaa gatgacact ccacgtaac 
gaagatagt gacgatgat gaacaatca cctccaaat 
aatcgtcga ttatatgac aaggccgtt gccggccgt 
gtgccaaaa atgaaacac gagcagaac aaagaatcg 
gacggaatc ggtccaaat atacgtagc attgttacc 
gttgtcata atgttattg ttgatgttg tttgcggta 
cattgtact tgggtcact agcaatgct tattcaagc 
ccaagtatc gtcctagca tcatacgga agtgacggt 
tcccgtcag attttggac gattttcgc gaagcttac 
ttttggttg tcgcaaaat acagccgac gaagcgaga 
gtgatgagt tggtgggat tacgggtat cagatcgca 
ggaatggcc aatcggacg acgttagtt gacaataac 
acgtggaac aacagtcac atcgccctg gtggggaag 
gcaatgtcg tccaatgag agcgcagct tacgagatc 
atgaccgcc cttgatgtg gactacgtc ctagtgatc 
ttcggcggt gtgattggc tattcgggc gatgatatt 
aacaagttt ttgtggatg gtgcggatc gcagaaggc 
gaacatccc aaagatata agagagagt gattatttt 
acggaacat ggcgagttt agagtggat gctgaaggg 
gcgccaact ctgttaaat tcactcatg tataagttg 
agttattat cgatttggt gaattgaag ttggattat 
cgtacaccg gctgggtat gatagaacc aggaacgca 
attattggt aataaagat ttccaactt acctacctg 
gaggaggcg tatacgact gagcattgg ctcgtgagg 
gtatacagg gtgaagaaa ccagacgag tttaatagg 
ccgaggatt cccgtggcg aatcgggtg gtcaaaagc 
cagaatttc gtatcgaaa aagagcacc aaacgcaaa 
aagggtaca attaaaaac aagccagtc atcgtgaag 
ggcaaaaga ctgtcaaaa caggcgtaa
    mRNA sequence
    • >TC009183 RA
      ctcatcaag taacctctg agtgtgtgt atgcgtgtc 
atgttttta cctgtcaaa aatggctca agccggttc 
gaaaaagtc ccaattaac caaaataaa tagtaaaac 
cggaataaa cagtcttat aattttcgt cgttctcct 
cctggcatg gatcgccgg cttttcgtc ccgtctctt 
cgccgtgat ccgtttcga aagcataat tcacgaatt 
tgatccatg gttcaatta ccgcgccac ggcgtacat 
ggtcaaaca cggctttta caatttttt gaactggtt 
tgatgagcg cgcctggta ccccttggg gcgcatcgt 
ggggggcac ggtgtaccc cggattgat gatcacgtc 
agggagcat acactacgt gttgcattt attgaacat 
caatgtaca tattaggga tatttgtgt gttcctggc 
ccccatctt cagcggcct cacggccat ctcgaccta 
ctttctgac caaggagct gtggtcggc gggggccgg 
ccttttcgc ggcgtgttt tattgccat tgtgcctgg 
atacatcag tcgttctgt tgccggaag ttacgataa 
tgaaggtat cgcaatttt cgcacttca attcactta 
tttcctctg gattaagtc ggtgaagac ggggtcggt 
gttttgggc cgtcgcagc ggcactttc gtactttta 
tatggtctc cgcatgggg aggctacgt gttcatcat 
caatctaat cccgcttca tgtgtttgt cctccttct 
gatgggacg attttcgaa ccgattatt taccagtta 
caccacttt ctacatact tgggttgat ttgctccat 
gcagatacc atttgttgg gttccaacc cattcgtac 
cagcgaaca catggcggc ttctggtgt ttttgcatt 
acttttcgc cgttgcgac gctaaaata cctacaaac 
gatgctctc aaagtctga gtttaagtc gttggtgat 
ttttggtgg tttaatatc ggcaggtgt tgtttttct 
aacagtggt aggactgac ctatgctgg gtatattgc 
accatggag tggccggtt ttattcatt gtgggatac 
tggatatgc taaaattca tattccaat aattgcgtc 
ggtttctga acatcaacc gaccacttg gttctcgtt 
tttctttga tttgcatat tttagttgc aacttttcc 
cgctggtct gtggtactg tatcaaaaa tgtgaacga 
cgagagggt tttcgtgat tctgtacgc cctgagtgc 
ggtttattt cgccggcgt tatggtccg tctcatgtt 
aaccctaac acctgtggt gtgcatttt gagcggaat 
agcattttc cggtctttt ggagctatt tttacgcga 
agatgacac tccacgtaa cgaagatag tgacgatga 
tgaacaatc acctccaaa taatcgtcg attatatga 
caaggccgt tgccggccg tgtgccaaa aatgaaaca 
cgagcagaa caaagaatc ggacggaat cggtccaaa 
tatacgtag cattgttac cgttgtcat aatgttatt 
gttgatgtt gtttgcggt acattgtac ttgggtcac 
tagcaatgc ttattcaag cccaagtat cgtcctagc 
atcatacgg aagtgacgg ttcccgtca gattttgga 
cgattttcg cgaagctta cttttggtt gtcgcaaaa 
tacagccga cgaagcgag agtgatgag ttggtggga 
ttacgggta tcagatcgc aggaatggc caatcggac 
gacgttagt tgacaataa cacgtggaa caacagtca 
catcgccct ggtggggaa ggcaatgtc gtccaatga 
gagcgcagc ttacgagat catgaccgc ccttgatgt 
ggactacgt cctagtgat cttcggcgg tgtgattgg 
ctattcggg cgatgatat taacaagtt tttgtggat 
ggtgcggat cgcagaagg cgaacatcc caaagatat 
aagagagag tgattattt tacggaaca tggcgagtt 
tagagtgga tgctgaagg ggcgccaac tctgttaaa 
ttcactcat gtataagtt gagttatta tcgatttgg 
tgaattgaa gttggatta tcgtacacc ggctgggta 
tgatagaac caggaacgc aattattgg taataaaga 
tttccaact tacctacct ggaggaggc gtatacgac 
tgagcattg gctcgtgag ggtatacag ggtgaagaa 
accagacga gtttaatag gccgaggat tcccgtggc 
gaatcgggt ggtcaaaag ccagaattt cgtatcgaa 
aaagagcac caaacgcaa aaagggtac aattaaaaa 
caagccagt catcgtgaa gggcaaaag actgtcaaa 
acaggcgta agtagttgt tgttagttg tttggaacg 
aagagtttt gttgattta gttctgcca gaaaagaag 
gtttttcac ttgtgataa ctgtgtaaa ataaaggta 
ataaatctt tttgcactg taaagtgtt ttgttcgac 
attaaacat cactttgtg aaattggtt gtatttttt 
tattaacat ttgtttatc ctagtatgt acagttgag 
tttgttaca taaacaact taagaaact acacgtaca 
cttagtaaa tggttgtag cggttacaa atcggtttt 
ttgtttgtt tgaaacgaa aaggccttt gtcaacctt 
attgcggcg tggaagatt cctcaatac aaaatataa 
atccaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY