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Gene information
General information
TC009182 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3350641..3353234
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009182
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01491

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber complete not present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 8 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (41 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009182 RA
      MEINDDSNE QMSVNSDSD SDSGDDQEE QALLTRAED 
      LERQISDNK YLYNAHEEL VNLYRKLGD LKSMRLAYE 
      RFQEYFPLT PEIWLAWIN DEKKLASSE SEIKNIFGL 
      FDKAVEDYL SVNLWVEYA QYSIGVSTL DTTRVIIER 
      GLTAAGLHV ADGSLLWDT LRELEFAHL SLTEAGSEP 
      WKTQVEKIV EVFRRQLSV PLLDMENTY KEWKEWLTQ 
      VQDHKINAE PVEWGYNKA LKTLETYKP FEEKLQVTT 
      DESQLLRVY RDYVKVLTD PSTILCLYE RATAQMCLN 
      PEIWLDYCL YGLKLGAPA EKITTKALR NCPWSQELW 
      IMRLQVLES LQKDNSEVV ACFEQAVSN LGAQSSELW 
      LAYLEYVSR TCQDQAKVF KSFDQALEQ LKYNDEGAL 
      KITKWYSRI LAKNNNMQG ARNLWKNLL KSPNHKILA 
      NTWLEYARL ERQFGEPPQ VRAIFQKAL TNCTDWPLF 
      IADEWREFE REFGKLPDV LKCLEKRKE IEPKLKTIE 
      PPKATGTKR KHPSEAEPP PKKHKKQAV VKDPTRTIF 
      VSNLHTSVN EKRLRKFFP NCENVEIVV DKRGKSRCY 
      GYVQFALEE SVMAALARD RELLDGRPI FISNCKPNR 
      EERKAGFKY SNEPETNKL FVKGLPYDK SQEEIEAIF 
      RPFGAKTVR LVCRRDGKP KGLAYVEFE DDASAKKAM 
      EKTDGMTVG DFTISVAIS APPVKKPTT VAKSTPTRH 
      ARSRLQVPM LPRSLQVKS ATNGDVKPK TNDDFRLLL 
      QK
    CDS sequence
    • >TC009182 RA
      atggaaata aacgacgat tccaacgag caaatgtcg 
      gtcaactcg gactcggac tccgactcc ggggacgac 
      caagaggag caagccctc ctcactcgt gctgaagac 
      ctcgaacgc caaatctcc gacaacaag tacctctac 
      aatgcccac gaagagctc gtcaacttg taccgaaaa 
      ctgggggac ttgaagtcg atgcgtctg gcgtacgaa 
      cgcttccaa gagtacttc cccctaacc cccgagatc 
      tggctcgcg tggatcaac gacgagaaa aagttagcg 
      agctccgag agcgagatt aagaatatt ttcgggctt 
      tttgataaa gccgtggag gattatttg tctgtgaat 
      ttgtgggtg gagtacgcg cagtactcg attggggtt 
      agcaccctt gacacgaca cgtgtgata atcgagagg 
      ggtttaacc gccgctggg ttacacgtg gccgatggg 
      agcctcctg tgggacact ctccgggag ctggaattc 
      gcccatttg tccctgacg gaggcaggg agcgagcca 
      tggaaaacc caagtggaa aaaatcgtg gaggttttc 
      cggcgccaa ctctcagtg ccgctcctt gacatggaa 
      aacacttac aaggagtgg aaagagtgg ctaacgcag 
      gtgcaagac cacaaaatc aatgctgaa ccggtggag 
      tggggctat aacaaggct ttgaaaacc ttagagacc 
      tacaagccg ttcgaagaa aaactacaa gtaacaacg 
      gacgaatcg caactatta cgagtttat cgcgactac 
      gtcaaagtc ctaacggac ccctcgaca attttgtgt 
      ctttatgaa cgcgcaacg gcccagatg tgtctcaac 
      ccggagatt tggctcgat tattgtctc tacggcctg 
      aaactggga gccccagct gaaaaaatc acaactaag 
      gctttgaga aattgcccc tggagtcaa gaattgtgg 
      attatgcgc ctacaagtc cttgaaagt ctccagaag 
      gataatagc gaagttgtc gcttgtttc gagcaggct 
      gtgtcaaat ttaggcgcc caatcaagc gagctgtgg 
      ctcgcctac ttagaatac gtctcacgg acgtgccaa 
      gaccaagcc aaagttttc aaatctttt gaccaagcc 
      ttagaacaa ctaaaatat aacgacgaa ggcgccctc 
      aaaatcaca aagtggtat tcgagaatt ttggccaaa 
      aataacaac atgcaaggg gcgcgaaac ctctggaaa 
      aacctactc aaaagcccg aaccacaaa atcctggcc 
      aacacttgg ctggagtac gcgcggctt gaacgccag 
      ttcggggaa cccccgcaa gtccgcgcc attttccag 
      aaagcccta accaattgc accgactgg cccctgttt 
      atcgccgac gaatggcgt gagttcgaa cgcgagttc 
      ggtaaacta cccgacgtt ctaaaatgc ctcgaaaaa 
      cgcaaagaa atcgaaccg aaactaaaa acgattgag 
      ccgccgaaa gccaccgga accaaacga aaacacccg 
      agcgaagcc gaacccccg cctaagaaa cacaaaaag 
      caggcggtg gtcaaagac ccgactcgc acaattttc 
      gtcagtaat ctacacact agtgtgaat gaaaaacga 
      ctgaggaaa ttcttcccc aattgcgaa aatgtcgaa 
      attgtggtc gataagcgg gggaagtcc cggtgttat 
      gggtacgtg cagttcgcc cttgaggag agtgtcatg 
      gcggcgctg gcacgtgac cgggagctg cttgacggc 
      cgccctatt tttatttcc aattgtaag ccgaatcgt 
      gaggagcgg aaagccggc tttaagtac tcgaatgaa 
      cccgagacc aataaactt ttcgttaag ggactcccc 
      tatgacaag tcgcaggag gaaattgag gcgattttt 
      cggcctttt ggggcgaaa accgtgcgt ttggtgtgt 
      cggcgagat ggtaagcca aaaggactc gcgtatgtt 
      gagtttgag gacgatgca agtgctaag aaagccatg 
      gaaaagacc gatgggatg acagtcggg gactttacc 
      atcagtgtg gcaattagc gcgccccct gtcaagaaa 
      ccaacaaca gttgcaaaa agtacacca acacggcat 
      gcacgcagc cgattacaa gtcccgatg ttaccgagg 
      tccttgcaa gtcaagagt gcgacgaat ggtgatgtc 
      aaaccgaaa accaatgat gatttccga ttgttactt 
      cagaaataa
    mRNA sequence
    • >TC009182 RA
      gtttgccaa catcaacat aacccaact ttcctattt 
      gtcaaaacg tacttttta cgatggaaa taaacgacg 
      attccaacg agcaaatgt cggtcaact cggactcgg 
      actccgact ccggggacg accaagagg agcaagccc 
      tcctcactc gtgctgaag acctcgaac gccaaatct 
      ccgacaaca agtacctct acaatgccc acgaagagc 
      tcgtcaact tgtaccgaa aactggggg acttgaagt 
      cgatgcgtc tggcgtacg aacgcttcc aagagtact 
      tccccctaa cccccgaga tctggctcg cgtggatca 
      acgacgaga aaaagttag cgagctccg agagcgaga 
      ttaagaata ttttcgggc tttttgata aagccgtgg 
      aggattatt tgtctgtga atttgtggg tggagtacg 
      cgcagtact cgattgggg ttagcaccc ttgacacga 
      cacgtgtga taatcgaga ggggtttaa ccgccgctg 
      ggttacacg tggccgatg ggagcctcc tgtgggaca 
      ctctccggg agctggaat tcgcccatt tgtccctga 
      cggaggcag ggagcgagc catggaaaa cccaagtgg 
      aaaaaatcg tggaggttt tccggcgcc aactctcag 
      tgccgctcc ttgacatgg aaaacactt acaaggagt 
      ggaaagagt ggctaacgc aggtgcaag accacaaaa 
      tcaatgctg aaccggtgg agtggggct ataacaagg 
      ctttgaaaa ccttagaga cctacaagc cgttcgaag 
      aaaaactac aagtaacaa cggacgaat cgcaactat 
      tacgagttt atcgcgact acgtcaaag tcctaacgg 
      acccctcga caattttgt gtctttatg aacgcgcaa 
      cggcccaga tgtgtctca acccggaga tttggctcg 
      attattgtc tctacggcc tgaaactgg gagccccag 
      ctgaaaaaa tcacaacta aggctttga gaaattgcc 
      cctggagtc aagaattgt ggattatgc gcctacaag 
      tccttgaaa gtctccaga aggataata gcgaagttg 
      tcgcttgtt tcgagcagg ctgtgtcaa atttaggcg 
      cccaatcaa gcgagctgt ggctcgcct acttagaat 
      acgtctcac ggacgtgcc aagaccaag ccaaagttt 
      tcaaatctt ttgaccaag ccttagaac aactaaaat 
      ataacgacg aaggcgccc tcaaaatca caaagtggt 
      attcgagaa ttttggcca aaaataaca acatgcaag 
      gggcgcgaa acctctgga aaaacctac tcaaaagcc 
      cgaaccaca aaatcctgg ccaacactt ggctggagt 
      acgcgcggc ttgaacgcc agttcgggg aacccccgc 
      aagtccgcg ccattttcc agaaagccc taaccaatt 
      gcaccgact ggcccctgt ttatcgccg acgaatggc 
      gtgagttcg aacgcgagt tcggtaaac tacccgacg 
      ttctaaaat gcctcgaaa aacgcaaag aaatcgaac 
      cgaaactaa aaacgattg agccgccga aagccaccg 
      gaaccaaac gaaaacacc cgagcgaag ccgaacccc 
      cgcctaaga aacacaaaa agcaggcgg tggtcaaag 
      acccgactc gcacaattt tcgtcagta atctacaca 
      ctagtgtga atgaaaaac gactgagga aattcttcc 
      ccaattgcg aaaatgtcg aaattgtgg tcgataagc 
      gggggaagt cccggtgtt atgggtacg tgcagttcg 
      cccttgagg agagtgtca tggcggcgc tggcacgtg 
      accgggagc tgcttgacg gccgcccta tttttattt 
      ccaattgta agccgaatc gtgaggagc ggaaagccg 
      gctttaagt actcgaatg aacccgaga ccaataaac 
      ttttcgtta agggactcc cctatgaca agtcgcagg 
      aggaaattg aggcgattt ttcggcctt ttggggcga 
      aaaccgtgc gtttggtgt gtcggcgag atggtaagc 
      caaaaggac tcgcgtatg ttgagtttg aggacgatg 
      caagtgcta agaaagcca tggaaaaga ccgatggga 
      tgacagtcg gggacttta ccatcagtg tggcaatta 
      gcgcgcccc ctgtcaaga aaccaacaa cagttgcaa 
      aaagtacac caacacggc atgcacgca gccgattac 
      aagtcccga tgttaccga ggtccttgc aagtcaaga 
      gtgcgacga atggtgatg tcaaaccga aaaccaatg 
      atgatttcc gattgttac ttcagaaat aaaagcgat 
      tcaaacata tttattcaa caactctct acctctaga 
      tacaaatga ggtaaccgc cctcaatat gctggcagt 
      ggtgggtga tttcgcaca tgtttctcc tgtttgagt 
      ggctgaaaa ttatctaaa aaatatata cgatacaca 
      aaa
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