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Gene information
General information
TC009177 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 1303889..1305304
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009177
    iBeetle screen iB_01490
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009177 RA
      MANILALLA ASSAFLALA SAGNVVCYF TSWTIYRPD 
      NGKFTALDV DPTLCTHLL YAFVGLRDD GVVSVLDDW 
      EITGLDEMN HLMSLREKN PDLKIILSM GGWNEGSYK 
      YSTVAKSAA LRATMVQSV LNFTDVWGF DGFDLDWEY 
      PCQRGGADE DKVNFVTLL GELKSALNA KGKTLSAAV 
      SGGIASCKL SYDIAKVAE NLDMINVMA YDFHGAFEP 
      FVGHYAPLY ASHLDQTDE QKTLNVAAG IQYWLDEGA 
      PPSKINLGL GTYGRGFAL ADPTNTSLY AGTYGGSDA 
      GPYTREMGV IGYNEVCEL YSDWDYFWD DEQQVPHIV 
      KGNQWLGFD DPKSIQVKV EFANEKNLG GVMVWSLDT 
      DDFRGICGK GPYPIMNAI KQTIK
    CDS sequence
    • >TC009177 RA
      atggcaaac atcttggct ctccttgct gcttccagt 
      gcctttctg gcactagcc tcggctgga aacgtggtc 
      tgttatttt accagctgg accatctac aggcccgac 
      aacggcaag tttaccgcc cttgacgtg gaccccacg 
      ctttgcacc caccttttg tacgcgttt gtcggtctt 
      cgagatgat ggagtcgta tcagttctc gatgactgg 
      gaaatcacc ggattagat gaaatgaac catttgatg 
      agtctgagg gagaaaaac cccgacttg aagattatc 
      ctaagtatg ggagggtgg aacgaaggc tcttataaa 
      tactcaact gttgccaaa agtgcggct ttgagggcg 
      accatggta cagtcagtc ctcaatttt accgatgtt 
      tggggtttc gatgggttt gaccttgac tgggaatac 
      ccatgtcaa aggggaggt gctgatgag gacaaagtc 
      aattttgtc actcttttg ggcgaattg aaatcggct 
      ttgaacgct aaaggaaag acgctttca gcggctgtg 
      tccggagga atcgcttcc tgcaagctt tcgtacgac 
      attgccaaa gttgcagag aacttggac atgatcaac 
      gtcatggcc tacgacttc catggcgct tttgagcct 
      tttgtgggc cattacgcc ccactttac gcctcccat 
      ttggaccaa accgacgag caaaaaacc ctcaatgtc 
      gccgctggt atccagtac tggctcgat gaaggagca 
      cctcccagt aaaatcaac ctgggtttg gggacttac 
      ggacgtggg ttcgctttg gctgacccc accaacacc 
      tctctgtac gctggtact tacggtggt agcgacgct 
      gggccttac acccgtgag atgggagtg attgggtac 
      aacgaagtg tgcgagctt tacagcgat tgggattat 
      ttctgggat gacgaacaa caagtgcca catattgtg 
      aaagggaac cagtggctg ggtttcgat gatcccaag 
      tcgattcaa gtcaaggtt gagtttgct aatgagaaa 
      aatctgggt ggggttatg gtatggtcg ttggacacc 
      gatgacttc agggggatt tgcggcaaa gggccgtat 
      cccattatg aatgcgatt aagcagacc attaaataa
    mRNA sequence
    • >TC009177 RA
      accgactta aacaatgca aaattgttg tctttggcg 
      acatggcaa acatcttgg ctctccttg ctgcttcca 
      gtgcctttc tggcactag cctcggctg gaaacgtgg 
      tctgttatt ttaccagct ggaccatct acaggcccg 
      acaacggca agtttaccg cccttgacg tggacccca 
      cgctttgca cccaccttt tgtacgcgt ttgtcggtc 
      ttcgagatg atggagtcg tatcagttc tcgatgact 
      gggaaatca ccggattag atgaaatga accatttga 
      tgagtctga gggagaaaa accccgact tgaagatta 
      tcctaagta tgggagggt ggaacgaag gctcttata 
      aatactcaa ctgttgcca aaagtgcgg ctttgaggg 
      cgaccatgg tacagtcag tcctcaatt ttaccgatg 
      tttggggtt tcgatgggt ttgaccttg actgggaat 
      acccatgtc aaaggggag gtgctgatg aggacaaag 
      tcaattttg tcactcttt tgggcgaat tgaaatcgg 
      ctttgaacg ctaaaggaa agacgcttt cagcggctg 
      tgtccggag gaatcgctt cctgcaagc tttcgtacg 
      acattgcca aagttgcag agaacttgg acatgatca 
      acgtcatgg cctacgact tccatggcg cttttgagc 
      cttttgtgg gccattacg ccccacttt acgcctccc 
      atttggacc aaaccgacg agcaaaaaa ccctcaatg 
      tcgccgctg gtatccagt actggctcg atgaaggag 
      cacctccca gtaaaatca acctgggtt tggggactt 
      acggacgtg ggttcgctt tggctgacc ccaccaaca 
      cctctctgt acgctggta cttacggtg gtagcgacg 
      ctgggcctt acacccgtg agatgggag tgattgggt 
      acaacgaag tgtgcgagc tttacagcg attgggatt 
      atttctggg atgacgaac aacaagtgc cacatattg 
      tgaaaggga accagtggc tgggtttcg atgatccca 
      agtcgattc aagtcaagg ttgagtttg ctaatgaga 
      aaaatctgg gtggggtta tggtatggt cgttggaca 
      ccgatgact tcaggggga tttgcggca aagggccgt 
      atcccatta tgaatgcga ttaagcaga ccattaaat 
      aaaacaatg agaataaat tttttaagt attt