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Gene information
General information
TC009166 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 1496040..1499406
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009166
    iBeetle screen iB_01485
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 22.22% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009166 RA
      MLAVFFLFL CVSASTQGT PSVIIIGAG PAGIAAASK 
      LFENSFTNL LILEAENRI GGRINSVKL GEKYVDLGA 
      EYCHGQKGN IVYELVKDL NVLAPISSH FKPALYYSN 
      GSRLQDSFT EELQAIILG YDDFETNSN FSGRSVGEV 
      FTSRYNATI MKKYEGDAE KIKLLKEAL RLAEKVSLM 
      IDGAFSWLE TSPVKHYVR SEGHQLLVW QGLGYRTIL 
      QVLMGEFPD KKSPIREKI RLNSPITQI RYHNSSKIV 
      VTTTNGSYE ADHVIFTPS VGVLKREKD TLFQPPLPE 
      KKLQAIEAL GIAGVMKIV LHFENEWWG DQDSIFTFL 
      WGEEDLGNL MGELKWVQS VALVAKVPG NPGVLVAWV 
      TGGLIPEME KMSEDDLLK GCVFLLEKF LGRDYNITT 
      PDKILKSTW HTNGHFRGT YSYERAGFE GATRYQSLL 
      AAPLESPEG KPAILFAGE ASNPAHYST VHGAIESGF 
      REASRLIKL YRKN
    CDS sequence
    • >TC009166 RA
      atgttggcc gtttttttc ctcttcttg tgtgtgagt 
      gcctcaacc caaggcacc ccttccgtc atcataatc 
      ggagccggc cctgcagga atcgccgcc gcttcgaaa 
      cttttcgaa aactccttc acaaaccta ctaatcctc 
      gaagccgaa aaccgaatc gggggccga ataaactcg 
      gtcaaactc ggcgaaaaa tacgtcgat ttgggggct 
      gagtactgc cacggccaa aagggcaac atcgtctac 
      gaactggtc aaagacttg aacgttctg gcgcccatt 
      tctagccac tttaagccg gccctgtac tactcgaac 
      gggtccaga ttgcaggac tccttcacc gaggagctc 
      caagccatt attttaggt tacgatgat ttcgagacc 
      aattcgaat ttttccggt cgcagtgtg ggcgaggtt 
      ttcacgtcg agatacaac gccacaatt atgaaaaag 
      tatgagggc gacgcggaa aaaatcaaa ttattaaaa 
      gaagcactt agactggct gagaaagtg tcactgatg 
      attgatggt gcgttttcc tggctcgaa acttcgcca 
      gtgaaacac tacgtgagg agcgaggga caccagttg 
      ctagtctgg caagggcta ggctatcgc accatttta 
      caagtcttg atgggggaa ttccccgac aagaaatcc 
      ccaatcagg gaaaaaatt cgactaaac tcgcccatc 
      acccaaata cgttaccac aattcgagc aaaattgtt 
      gtaaccaca actaatggg agttacgag gccgatcat 
      gtcattttc accccttcc gtgggggtt ctgaagcgt 
      gaaaaagac accctgttc caacccccc ctccctgaa 
      aaaaaatta caagcgatc gaggcgctt ggtattgca 
      ggtgttatg aaaattgtt ctacatttt gagaacgag 
      tggtggggg gatcaagac tcgattttt acttttttg 
      tggggtgag gaggattta gggaatttg atgggggag 
      ttgaagtgg gtgcaaagt gtggcactt gtggccaaa 
      gttccgggg aatcccggc gttttagtg gcatgggtc 
      actggcggg ttaatcccc gaaatggaa aaaatgtca 
      gaagatgat ttgttaaaa gggtgtgtt tttctgttg 
      gagaagttt ctagggagg gattacaac atcacgact 
      cctgataaa attctaaag tcgacttgg cacacaaac 
      ggccacttc aggggcact tattcgtac gaaagggcc 
      ggtttcgag ggggccacc cgttaccag tcgcttctg 
      gcagcccct ctggagtcg ccggagggc aaacctgcg 
      attttattc gctggggaa gcttcaaat cctgctcat 
      tattcgaca gttcacggt gcgattgaa agcggcttt 
      cgtgaagct tcacgtctg ataaagttg tatcgtaaa 
      aattaa
    mRNA sequence
    • >TC009166 RA
      tctcaaaaa tgaggcaat atttttatc aaacaagcc 
      aaaaggtta ccaataaat cattcccca gtttgtaac 
      cttccacac aaaatgttg gccgttttt ttcctcttc 
      ttgtgtgtg agtgcctca acccaaggc accccttcc 
      gtcatcata atcggagcc ggccctgca ggaatcgcc 
      gccgcttcg aaacttttc gaaaactcc ttcacaaac 
      ctactaatc ctcgaagcc gaaaaccga atcgggggc 
      cgaataaac tcggtcaaa ctcggcgaa aaatacgtc 
      gatttgggg gctgagtac tgccacggc caaaagggc 
      aacatcgtc tacgaactg gtcaaagac ttgaacgtt 
      ctggcgccc atttctagc cactttaag ccggccctg 
      tactactcg aacgggtcc agattgcag gactccttc 
      accgaggag ctccaagcc attatttta ggttacgat 
      gatttcgag accaattcg aatttttcc ggtcgcagt 
      gtgggcgag gttttcacg tcgagatac aacgccaca 
      attatgaaa aagtatgag ggcgacgcg gaaaaaatc 
      aaattatta aaagaagca cttagactg gctgagaaa 
      gtgtcactg atgattgat ggtgcgttt tcctggctc 
      gaaacttcg ccagtgaaa cactacgtg aggagcgag 
      ggacaccag ttgctagtc tggcaaggg ctaggctat 
      cgcaccatt ttacaagtc ttgatgggg gaattcccc 
      gacaagaaa tccccaatc agggaaaaa attcgacta 
      aactcgccc atcacccaa atacgttac cacaattcg 
      agcaaaatt gttgtaacc acaactaat gggagttac 
      gaggccgat catgtcatt ttcacccct tccgtgggg 
      gttctgaag cgtgaaaaa gacaccctg ttccaaccc 
      cccctccct gaaaaaaaa ttacaagcg atcgaggcg 
      cttggtatt gcaggtgtt atgaaaatt gttctacat 
      tttgagaac gagtggtgg ggggatcaa gactcgatt 
      tttactttt ttgtggggt gaggaggat ttagggaat 
      ttgatgggg gagttgaag tgggtgcaa agtgtggca 
      cttgtggcc aaagttccg gggaatccc ggcgtttta 
      gtggcatgg gtcactggc gggttaatc cccgaaatg 
      gaaaaaatg tcagaagat gatttgtta aaagggtgt 
      gtttttctg ttggagaag tttctaggg agggattac 
      aacatcacg actcctgat aaaattcta aagtcgact 
      tggcacaca aacggccac ttcaggggc acttattcg 
      tacgaaagg gccggtttc gagggggcc acccgttac 
      cagtcgctt ctggcagcc cctctggag tcgccggag 
      ggcaaacct gcgatttta ttcgctggg gaagcttca 
      aatcctgct cattattcg acagttcac ggtgcgatt 
      gaaagcggc tttcgtgaa gcttcacgt ctgataaag 
      ttgtatcgt aaaaattaa ttttggggt tgaataaag 
      tgataaatg agta