General information | |||
TC009163 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 3591136..3593616 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC009163 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01484 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC009163 RA
MIHEILIDL LHNSDDSLG KLDELQEFL HPGEKQLLH HIMEISSNH NWIVSFCKK IQNCSVDQH GLYIKAFVN GVLDVLGDY KSEIVRLED LFLQNPELQ LIFISSCLE KYQVLFNVI MSIIQVVEK DRLHGCLLM GRLHKYIYC GVEPITQAA KKITHCFNS IFYRHLCNW IIYGDLVDL YKEFFISDG ECWDEDFLY PEQNEESTL TRVKHRIKR PPPVRKFTI VWSMVPMFI TEDTAESIL FMGRIVWIV KNHQKTNTD NYQNLHKPD VWDGKDIEY YKKIQALET QIFDQMEFQ RVIEECRIK LTKYLWSVM VDEGDLKEH LQLIRDYYA LGRGELFHQ FIEAAENQT TSYNLNVIF LETARKIYG ENDKTYLRF ELAKLEKTS VNPWSKLQL NFEISWPLH IIFNPKSME LYNQLFCYL LRLSRTQIH LHKLWQAHM SGKQNIDRR VWTLRQHLI FLVNNLQYY LQVDVIEAQ FSLLLKAIE NASEFEEII KVHHEFISN LLAKTFILT PDKSHTYQN KHRLHQTPA VQFNVPSEV YNVIIQLLE LCDNFCVIA STWQPQLTE PEVTEFEQF QKRTDTIVD SLLFILYKL HERADGQHL VQLLHQLDF NRFFTRNKP EFNLTQRT
- >TC009163 RA
atgattcac gagatactg attgacttg ttgcacaat tcggacgat tcgttgggc aaacttgac gaactccag gagttcctg cacccgggg gagaagcaa ctcctccac cacatcatg gaaatatcc tcaaaccac aactggatc gtctcattt tgcaaaaaa atccagaac tgtagtgtt gaccaacac ggtctctac atcaaggct tttgtgaac ggtgtgctt gatgttttg ggggattac aagagcgaa attgtcaga ctggaagat ctattcttg caaaacccc gagcttcag ttaattttc atttcaagt tgcttggaa aagtaccaa gtcttgttc aatgtgatt atgtcgatc attcaagtc gtggagaag gacaggctc catgggtgc ctcctgatg gggcggctc cacaaatac atctactgt ggcgtggaa cctataacc caagctgcg aaaaagata actcactgc ttcaacagc attttttac cgccacttg tgcaactgg atcatttac ggcgacttg gtcgacctt tataaagag tttttcatc agcgatggc gaatgctgg gacgaggat ttcctctac cctgagcaa aacgaagaa agcacactg acacgagtt aaacaccga atcaagcgc ccaccacct gtccgcaaa ttcacaata gtctggtct atggttccg atgtttatc actgaagat acggccgaa agcatactt ttcatgggc cgaatcgta tggatcgtc aaaaaccac cagaaaacc aacacagac aattaccaa aacctacac aagcctgac gtttgggac ggcaaagat atagagtat tacaagaaa attcaagcg ctcgagacg caaattttc gaccagatg gagttccag agggttata gaggagtgc agaatcaaa ttaaccaaa tacttgtgg tccgttatg gtggacgag ggcgaccta aaggaacac ttgcaactt atacgagat tactatgcg ctggggcga ggcgaactt ttccatcaa ttcatcgaa gcggcggaa aatcaaacc acttcgtac aacctcaac gtgattttc ctggaaact gcgcggaaa atctacggc gagaacgac aagacgtac ctcagattc gaattagct aaactggaa aagacaagc gtgaacccg tggtcgaaa ttacaactc aatttcgaa attagttgg ccccttcat atcatcttc aacccaaaa tcgatggaa ctgtacaat caattattc tgctacttg ttaaggctg agcagaact cagatccac ttacacaag ttgtggcaa gcgcacatg tccgggaaa caaaacatt gaccggcga gtgtggact ttacgccag catttaatt ttcctagtc aataatcta caatattac ctccaagtg gacgtgatc gaggcgcaa ttttcgcta cttttgaaa gcgatcgaa aacgccagc gaattcgag gagatcatc aaagtccat cacgagttc atcagcaac ttactcgcg aaaactttc atcctgacc cctgataaa agccacact tatcaaaac aaacaccgg ttacaccaa actcccgcc gttcagttt aacgtcccg agtgaagtt tacaacgtg ataatccag ctgctggag ctgtgtgac aatttctgc gtcattgcg tcgacatgg caaccgcag ttaaccgaa ccggaagta accgagttt gaacagttc cagaaacgc accgatacc atcgtcgat tcgcttttg tttattctg tacaagtta catgaacga gccgatggc caacacttg gtccagttg ttgcaccag ctcgacttt aatcgcttt ttcactcga aacaaaccc gagtttaac ctaacgcaa agaacgtaa
- >TC009163 RA
tggcagcaa tttgctaat tcaaaaatt gatgttttg aggttaaat aattttatt ggtggtaaa aaaaggcca taatgattc acgagatac tgattgact tgttgcaca attcggacg attcgttgg gcaaacttg acgaactcc aggagttcc tgcacccgg gggagaagc aactcctcc accacatca tggaaatat cctcaaacc acaactgga tcgtctcat tttgcaaaa aaatccaga actgtagtg ttgaccaac acggtctct acatcaagg cttttgtga acggtgtgc ttgatgttt tgggggatt acaagagcg aaattgtca gactggaag atctattct tgcaaaacc ccgagcttc agttaattt tcatttcaa gttgcttgg aaaagtacc aagtcttgt tcaatgtga ttatgtcga tcattcaag tcgtggaga aggacaggc tccatgggt gcctcctga tggggcggc tccacaaat acatctact gtggcgtgg aacctataa cccaagctg cgaaaaaga taactcact gcttcaaca gcatttttt accgccact tgtgcaact ggatcattt acggcgact tggtcgacc tttataaag agtttttca tcagcgatg gcgaatgct gggacgagg atttcctct accctgagc aaaacgaag aaagcacac tgacacgag ttaaacacc gaatcaagc gcccaccac ctgtccgca aattcacaa tagtctggt ctatggttc cgatgttta tcactgaag atacggccg aaagcatac ttttcatgg gccgaatcg tatggatcg tcaaaaacc accagaaaa ccaacacag acaattacc aaaacctac acaagcctg acgtttggg acggcaaag atatagagt attacaaga aaattcaag cgctcgaga cgcaaattt tcgaccaga tggagttcc agagggtta tagaggagt gcagaatca aattaacca aatacttgt ggtccgtta tggtggacg agggcgacc taaaggaac acttgcaac ttatacgag attactatg cgctggggc gaggcgaac ttttccatc aattcatcg aagcggcgg aaaatcaaa ccacttcgt acaacctca acgtgattt tcctggaaa ctgcgcgga aaatctacg gcgagaacg acaagacgt acctcagat tcgaattag ctaaactgg aaaagacaa gcgtgaacc cgtggtcga aattacaac tcaatttcg aaattagtt ggccccttc atatcatct tcaacccaa aatcgatgg aactgtaca atcaattat tctgctact tgttaaggc tgagcagaa ctcagatcc acttacaca agttgtggc aagcgcaca tgtccggga aacaaaaca ttgaccggc gagtgtgga ctttacgcc agcatttaa ttttcctag tcaataatc tacaatatt acctccaag tggacgtga tcgaggcgc aattttcgc tacttttga aagcgatcg aaaacgcca gcgaattcg aggagatca tcaaagtcc atcacgagt tcatcagca acttactcg cgaaaactt tcatcctga cccctgata aaagccaca cttatcaaa acaaacacc ggttacacc aaactcccg ccgttcagt ttaacgtcc cgagtgaag tttacaacg tgataatcc agctgctgg agctgtgtg acaatttct gcgtcattg cgtcgacat ggcaaccgc agttaaccg aaccggaag taaccgagt ttgaacagt tccagaaac gcaccgata ccatcgtcg attcgcttt tgtttattc tgtacaagt tacatgaac gagccgatg gccaacact tggtccagt tgttgcacc agctcgact ttaatcgct ttttcactc gaaacaaac ccgagttta acctaacgc aaagaacgt aatttattt tttcttcat cactttcat aaataacac catatctgg aataaatta attaggagg aa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
