Status
Please wait ...
Gene information
General information
TC009157 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3665129..3669550
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009157
    iBeetle screen iB_01481

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs segment shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009157 RA
      MSSFVVAAL LAVAIATQA LEEDFAFDV SDDFEVEVN 
      AGNREIEAA VGKVFHYVV PQVAATGGF EARRSDGSP 
      LPSWLLFDK IGAVFWGVP LGDDVGSLH LVIRNRNIS 
      EDVTINVRE TSDLKDRCP STEDNTVLI LLIDKGVRA 
      IKPKQRVIA VNNVAKFFG LPYSAFTLK PQLEKDDIT 
      DSSVVLAGP GNFKPKSAK STSVKVAVG CDGRLWVQT 
      APLVHQLKQ QARDGTISE VLGLPLIGW RVKTEPKSS 
      RTKRQTEDY GSGDYDDDY YDNYEDEYE YEEEDTKSS 
      TTTTVSPTP THPHRHHHG ESLPEETHH QNQETSSQV 
      VPTTVEMVP ETTTTTTTP KPTTEETPL VTNATKIDN 
      SYEYEDYDY DLEENEEDP IESKIVVPE FVPKPSETT 
      EATTTTTAK STTIQVVET VPTVPTTTE RETTMMIIE 
      DTEPATVLY ETTNLIPET SPVATTDFS TTTATITTT 
      TEEEELPTT PSTTTRHVV TSPRTTEMT ESVELEEKN 
      FPPYIENRL KQMSVIAGK IFRFVVPKN TFNDFEDGH 
      NLTLEFLNS RGEPLSKDS WCHFNPHRR EIYGLPLEE 
      DVSKWEYVL KATDREGAS ITDVFTIQV QQHKLQRVV 
      NHEISLFMR IEKQQEFPH FVDWCLKTL RALGKIYNT 
      NMTEITVRN VNYTSEPVV FTWTNDSIP TNYCPVEEI 
      DKLYKMLTA NDRGDPKQE LSLLLTPDL RVKKVTKHG 
      LGVCETPQV PVTPPTNFS PFLRNPVDH INATVGELL 
      IFKVPDDTF YDPEDTDSR TLNISLLTG DRQAIKSTN 
      WLQFDSKNR EFYGIPRKP GRSEYHLVC VDSGGATAT 
      DSLEIVVYP AHKMHYNVE FSMTVETPY DTFVNSASM 
      QRKFVEKLQ EIFGDRNSS SLYFNPFKQ KDHSTVITW 
      FNRTLSTSK CPHDEINHL ESVLKNHQD RGISHQVSR 
      IMEPEFLVS KISVFTMGN CRTKSLPPP VHEDVIPVD 
      EKSPDASAA QYQYLMTFI IPAVIISIM LFFAALCAC 
      VLYRRRRTG KLNVEEDGR QSYGNKGIP VIFQEELEE 
      KPESGTKTP VILKDEKPP LAPPEYSKS GSLKLTDDS 
      EPYQPPPPF TRTDGRQPR PKPTPTYRK PPPYVPP
    CDS sequence
    • >TC009157 RA
      atgtcttcc ttcgtggtc gctgcactg ctggcggtg 
      gcgatcgcg acccaagcc ttagaagag gactttgcc 
      ttcgacgtc tccgacgac ttcgaggtg gaggtgaac 
      gccggcaac agggaaata gaggctgcg gtgggcaaa 
      gtgttccac tacgtcgtg ccccaagtg gctgccact 
      ggaggtttc gaggccaga cgctccgac ggctcgcct 
      ctcccttcg tggctactt ttcgataaa atcggagca 
      gttttctgg ggtgtgccg ctcggtgac gacgtcggt 
      agtctgcat ctagtcatc aggaataga aacatatct 
      gaggacgtg acgattaac gtgcgcgaa acgtcggat 
      ctcaaggac aggtgtccc agcactgaa gataacact 
      gtacttatc cttctcatt gataagggc gtcagggca 
      atcaaaccg aaacaaaga gtgatagct gttaacaac 
      gtcgcgaag ttttttggc ttgccttat agtgccttt 
      actttaaaa cctcaatta gaaaaagac gatattacg 
      gatagttcg gtagtacta gcaggtcca gggaatttc 
      aagccaaag tcggcgaaa tcaacttcg gtgaaagtg 
      gcagtgggc tgtgatggg cgtttgtgg gtgcaaaca 
      gccccactg gtccatcag ttgaagcaa caagcgcgt 
      gatggtacc ataagtgag gtactcggg cttccattg 
      atcggttgg agggtcaaa accgaacca aaatcttca 
      agaactaag cgacaaact gaagattat ggtagcggt 
      gattacgat gacgattat tacgacaac tacgaagat 
      gagtacgaa tatgaagaa gaagatact aagagtagt 
      accaccact actgtaagc ccgaccccg actcacccc 
      catcggcac caccatggt gagtcccta cctgaagaa 
      acgcaccac cagaaccag gagacctcg tcacaagtc 
      gtaccaaca accgtggaa atggtaccg gaaacaacc 
      acaaccaca acaacccct aaaccaaca actgaagaa 
      acgccactc gtgacaaac gccaccaaa atcgacaac 
      agctacgag tatgaagat tacgactac gatttggaa 
      gaaaacgaa gaggacccg attgagagc aagattgtt 
      gtacctgaa ttcgtacca aaaccaagc gagacgacc 
      gaagcaaca acaacaaca accgcaaaa tcaacaaca 
      attcaagtt gttgaaacg gtaccaaca gtacccaca 
      acaacagaa cgcgaaaca acaatgatg attattgag 
      gacacggaa ccggcgacc gtgttgtac gaaaccacg 
      aatttaatt ccggaaaca agtcccgtt gctacaacc 
      gatttttcc accacgaca gcaacaata acaacaacg 
      accgaagag gaagaattg cccacaaca ccaagtaca 
      acaacccgg catgttgta acttcgccc cgaaccacc 
      gaaatgacc gaatcggtt gaactcgaa gagaagaac 
      ttcccaccg tacatcgaa aaccggctc aagcaaatg 
      tcggtgatt gccgggaag atcttccgg ttcgtggtc 
      ccgaaaaac acgttcaac gatttcgaa gatgggcat 
      aatctcact ttggagttt ttgaattcg aggggtgag 
      cccctgtcg aaggactcc tggtgccat ttcaacccc 
      caccggcgc gagatctac ggcttgccc ctcgaggag 
      gacgtctcc aagtgggag tacgtcctg aaggcgacc 
      gaccgcgaa ggggcctcc atcacggac gtgttcacc 
      atccaggtg caacaacac aagctgcaa cgtgtcgtc 
      aaccacgaa atttcgctc tttatgcgg atcgagaaa 
      cagcaagag ttcccgcat ttcgtcgac tggtgtctg 
      aagactttg cgggccttg gggaagatt tacaacacg 
      aatatgacc gagatcacg gttcggaat gtgaattat 
      acgagcgaa ccggtggtt tttacgtgg acgaatgat 
      tcgatcccg acgaattat tgcccggtt gaggagatc 
      gacaagctt tataagatg ttgacggcc aacgatcgc 
      ggagaccct aaacaagag ctgagtctg ctgttaacc 
      cccgatttg agggtgaag aaggtgacc aagcacggg 
      ctgggggtg tgcgagacg cctcaagtc ccggtgact 
      cccccgacg aacttttcg ccctttttg cgcaatcct 
      gtagaccat attaatgcc acggttggc gagttgctc 
      attttcaaa gtccctgat gacactttt tacgatccg 
      gaagatacg gattcaagg actctcaat atttcgctc 
      ctgacgggc gatcgccag gcgatcaag tcgaccaat 
      tggttacag tttgacagt aagaatcgc gaattttat 
      gggattccc cgaaagcca ggacggagt gaataccac 
      ctggtttgt gtggatagt ggaggggct acagcgact 
      gatagtttg gaaattgtc gtctatcca gcgcacaaa 
      atgcactac aacgtcgag tttagcatg acggtggag 
      acgccgtat gacacgttt gtgaacagt gcttccatg 
      cagaggaaa tttgtggaa aagttacaa gaaatcttt 
      ggcgaccgc aactccagc tctctctac ttcaacccc 
      ttcaagcaa aaagaccac tcaacggtc atcacctgg 
      ttcaaccgc acgctcagt accagcaag tgcccccac 
      gacgaaatc aaccacctg gagtcggtg ctcaagaac 
      caccaagac cgcggcatc tcccaccag gtctcgcgc 
      atcatggag ccggaattc ctcgtctcg aaaatcagc 
      gtcttcacc atgggcaac tgccgcaca aaatccctc 
      ccgccgcca gtccacgag gacgtcatc cccgtggac 
      gaaaaatcc ccggacgcc tccgccgcc caataccag 
      tacctgatg accttcatc atccccgcg gtcatcatc 
      tccatcatg ctcttcttc gccgcgctg tgcgcctgc 
      gtgctgtac cgccgaagg cgcaccggg aagctgaac 
      gtcgaggag gacggccgc cagagctac ggcaacaag 
      ggaatcccc gtcattttc caggaggag ttggaggag 
      aagcccgaa tccgggacg aagacgccg gtgatttta 
      aaagacgag aagccgccg cttgcgccc ccggagtac 
      agcaaaagc ggctcgctg aagctgacc gacgactcg 
      gagccctac cagccgccg ccgccgttc acgcgcacc 
      gacggccgg cagccgcgc cccaagccc acccccacg 
      tacaggaag ccgcccccg tatgtgccc ccctaa
    mRNA sequence
    • >TC009157 RA
      gcgataaac aaacccaac agcgtctaa atattgcaa 
      tttccggtg attcaccta aaatacgtt tgcaggcaa 
      tgaatgcgt gacccttcc gtgagaatg tcttccttc 
      gtggtcgct gcactgctg gcggtggcg atcgcgacc 
      caagcctta gaagaggac tttgccttc gacgtctcc 
      gacgacttc gaggtggag gtgaacgcc ggcaacagg 
      gaaatagag gctgcggtg ggcaaagtg ttccactac 
      gtcgtgccc caagtggct gccactgga ggtttcgag 
      gccagacgc tccgacggc tcgcctctc ccttcgtgg 
      ctacttttc gataaaatc ggagcagtt ttctggggt 
      gtgccgctc ggtgacgac gtcggtagt ctgcatcta 
      gtcatcagg aatagaaac atatctgag gacgtgacg 
      attaacgtg cgcgaaacg tcggatctc aaggacagg 
      tgtcccagc actgaagat aacactgta cttatcctt 
      ctcattgat aagggcgtc agggcaatc aaaccgaaa 
      caaagagtg atagctgtt aacaacgtc gcgaagttt 
      tttggcttg ccttatagt gcctttact ttaaaacct 
      caattagaa aaagacgat attacggat agttcggta 
      gtactagca ggtccaggg aatttcaag ccaaagtcg 
      gcgaaatca acttcggtg aaagtggca gtgggctgt 
      gatgggcgt ttgtgggtg caaacagcc ccactggtc 
      catcagttg aagcaacaa gcgcgtgat ggtaccata 
      agtgaggta ctcgggctt ccattgatc ggttggagg 
      gtcaaaacc gaaccaaaa tcttcaaga actaagcga 
      caaactgaa gattatggt agcggtgat tacgatgac 
      gattattac gacaactac gaagatgag tacgaatat 
      gaagaagaa gatactaag agtagtacc accactact 
      gtaagcccg accccgact cacccccat cggcaccac 
      catggtgag tccctacct gaagaaacg caccaccag 
      aaccaggag acctcgtca caagtcgta ccaacaacc 
      gtggaaatg gtaccggaa acaaccaca accacaaca 
      acccctaaa ccaacaact gaagaaacg ccactcgtg 
      acaaacgcc accaaaatc gacaacagc tacgagtat 
      gaagattac gactacgat ttggaagaa aacgaagag 
      gacccgatt gagagcaag attgttgta cctgaattc 
      gtaccaaaa ccaagcgag acgaccgaa gcaacaaca 
      acaacaacc gcaaaatca acaacaatt caagttgtt 
      gaaacggta ccaacagta cccacaaca acagaacgc 
      gaaacaaca atgatgatt attgaggac acggaaccg 
      gcgaccgtg ttgtacgaa accacgaat ttaattccg 
      gaaacaagt cccgttgct acaaccgat ttttccacc 
      acgacagca acaataaca acaacgacc gaagaggaa 
      gaattgccc acaacacca agtacaaca acccggcat 
      gttgtaact tcgccccga accaccgaa atgaccgaa 
      tcggttgaa ctcgaagag aagaacttc ccaccgtac 
      atcgaaaac cggctcaag caaatgtcg gtgattgcc 
      gggaagatc ttccggttc gtggtcccg aaaaacacg 
      ttcaacgat ttcgaagat gggcataat ctcactttg 
      gagtttttg aattcgagg ggtgagccc ctgtcgaag 
      gactcctgg tgccatttc aacccccac cggcgcgag 
      atctacggc ttgcccctc gaggaggac gtctccaag 
      tgggagtac gtcctgaag gcgaccgac cgcgaaggg 
      gcctccatc acggacgtg ttcaccatc caggtgcaa 
      caacacaag ctgcaacgt gtcgtcaac cacgaaatt 
      tcgctcttt atgcggatc gagaaacag caagagttc 
      ccgcatttc gtcgactgg tgtctgaag actttgcgg 
      gccttgggg aagatttac aacacgaat atgaccgag 
      atcacggtt cggaatgtg aattatacg agcgaaccg 
      gtggttttt acgtggacg aatgattcg atcccgacg 
      aattattgc ccggttgag gagatcgac aagctttat 
      aagatgttg acggccaac gatcgcgga gaccctaaa 
      caagagctg agtctgctg ttaaccccc gatttgagg 
      gtgaagaag gtgaccaag cacgggctg ggggtgtgc 
      gagacgcct caagtcccg gtgactccc ccgacgaac 
      ttttcgccc tttttgcgc aatcctgta gaccatatt 
      aatgccacg gttggcgag ttgctcatt ttcaaagtc 
      cctgatgac actttttac gatccggaa gatacggat 
      tcaaggact ctcaatatt tcgctcctg acgggcgat 
      cgccaggcg atcaagtcg accaattgg ttacagttt 
      gacagtaag aatcgcgaa ttttatggg attccccga 
      aagccagga cggagtgaa taccacctg gtttgtgtg 
      gatagtgga ggggctaca gcgactgat agtttggaa 
      attgtcgtc tatccagcg cacaaaatg cactacaac 
      gtcgagttt agcatgacg gtggagacg ccgtatgac 
      acgtttgtg aacagtgct tccatgcag aggaaattt 
      gtggaaaag ttacaagaa atctttggc gaccgcaac 
      tccagctct ctctacttc aaccccttc aagcaaaaa 
      gaccactca acggtcatc acctggttc aaccgcacg 
      ctcagtacc agcaagtgc ccccacgac gaaatcaac 
      cacctggag tcggtgctc aagaaccac caagaccgc 
      ggcatctcc caccaggtc tcgcgcatc atggagccg 
      gaattcctc gtctcgaaa atcagcgtc ttcaccatg 
      ggcaactgc cgcacaaaa tccctcccg ccgccagtc 
      cacgaggac gtcatcccc gtggacgaa aaatccccg 
      gacgcctcc gccgcccaa taccagtac ctgatgacc 
      ttcatcatc cccgcggtc atcatctcc atcatgctc 
      ttcttcgcc gcgctgtgc gcctgcgtg ctgtaccgc 
      cgaaggcgc accgggaag ctgaacgtc gaggaggac 
      ggccgccag agctacggc aacaaggga atccccgtc 
      attttccag gaggagttg gaggagaag cccgaatcc 
      gggacgaag acgccggtg attttaaaa gacgagaag 
      ccgccgctt gcgcccccg gagtacagc aaaagcggc 
      tcgctgaag ctgaccgac gactcggag ccctaccag 
      ccgccgccg ccgttcacg cgcaccgac ggccggcag 
      ccgcgcccc aagcccacc cccacgtac aggaagccg 
      cccccgtat gtgcccccc taacaatta cacttcacc 
      ttttttcag caataatca gttactgcc agttttttt 
      cacgatgcc aaatctgct gctaccgct ttcactttg 
      tttgattat tatgtgata ttgcgttcg gatttatta 
      agctgagaa ccaaaattg tactttatg gcgagtagg 
      attaacgag aactaatag attaggtct tctaaggct 
      gtttttact ttattgctt taggtttcg acacggttt 
      actgtcgca caatctttt tttaactct gtacattta 
      ttttttatc catgtatct tgatattaa agcgttttt 
      atatttaag tttagttac caataactt tgtactgta 
      aaaatttat aatgtttta tataagact ttttgtaag 
      gatgtactc gaatcatat caataaggc tagagttta 
      tttgtaatc tatacgtaa ctgacttac ttttaaatg 
      gtttgaatg tatcatatc aataaatcc ccatatttg 
      tccccgacg atatcatac ttttgtatt attaacgag 
      tcattataa taataccta attgttcaa taaacacta 
      aaaacaaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY