Status
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Gene information
General information
TC031189
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009148,TC009149
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3801243..3809475
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031189
    iBeetle screen iB_01480

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031189 RA
      MKDSKLVLK EARDAIKNK NYETALKLC KQILKEEKN 
      NYMALVFLG VSLQETGQN AKALKAFQE AIEANPSNP 
      LAWNGLINY YEKIDTKET KLDLINAYI SLIGIETGE 
      KKILECVQK LANLCEFGD LAKIVTAIF SVLKEGKCD 
      SAECAVCLI NILSKIENL APDLVPIYE ETLKLALNN 
      QKTVCTKYF SDYLNLLYK QDKAGELLQ QAKKMHEMF 
      ESDWISLRW ICKIFNEVY VESGTLIES VENMANYSE 
      SLSKLEAPS ATGLFTQSI LLVEQKKII EAKDVLEQV 
      VGVLPGLVH AWVLLLKCC MQLLLYDEA INAANKLEK 
      LLQNKKILK KSVNVLMVE VLSRSEEEA DLRKALQLF 
      ENIETEVKS ACNQHLIRA NINLKKFTE AKSLLQEFN 
      STDKTHILL KAQLLHKQE KFSEALEIL ERENCESCE 
      WWLEIGSLY WDCGHFDKS LIPFLKATK LDPNNYKCF 
      LFLGHFYKR NNDFDKARR CYEKAFKIN KRCAEAGIE 
      LSKIYRKLK NWDANLNLL QALTSGIIN AKNSWAWMQ 
      LGLHHLEQL DYDKAVTNL RFVIRIDEN NAHCWESLA 
      DAYLAKGAY TSALKCYEK TSSLVSEAW YPMLQKGHI 
      KQILGEFAE ARLEFEEIL KFNKFYVPA LKGMAETCL 
      LQARHCYKD QRLGTSRDH AQVATDHLI RALSQRGDL 
      SCLWKLLGN GCLFVANLP EKHSCLLIA KKFVEGRDV 
      VGDTVLEKS DLFALAARC FFKSLGLVE DNFLIWHDL 
      AVCYLSHGL SSGSSELIT KAQTILQHC TANNPTYWP 
      HWNLLGNVA MHLNPPNYK FAQHCFIKA VIADSHSAV 
      SWTNLGTLY FLLGDFKLA NQAFSQAQR SDPNYVNSW 
      IGQALLAET LAHEDAMDL FRHSTQLRV HKQGALGYA 
      HWVCKTLKE SPVDTVVYS IHNMHAVPV ACDVITWYT 
      EHDPMNATA WNMLGILKE RMGLKLGAL EAFKNALTL 
      SDKKQRDLA RVNYGRLLA ALGKYQQAV DSFTKVEAA 
      TFNSGSGLA LALFKDKLY KESYEVYES TLHWLSDDS 
      NLQSNLLVA LASMAYMFE GPEAAKTLL FQSIQLENP 
      SPWGFYAIS SLGLLHDDF NLTDLVLSE LKKIISRSC 
      GPHFAVLAS YFHLLQGKN KQSVCEVSK IVFMYPDQA 
      PVWATLSSV LIRVGKDAK AAANCAQIA MKLGQATNT 
      NVTKILCLV ALSFLIVGD TAKALLMAQ KAIHCYPNL 
      AEGWAVFIA ALVRSKRTN RVPEFLKIA QQLNPLRVK 
      FVAMLQVTL WLIFALISS GFAQSCKTR GEWCADTYQ 
      CCGGCCIRN TCIDTYQDC RIISDPCLE FYCPPDEMC 
      RLYQPRCEG CEIEKRCVP AGATDTVMF MDPEKLPSA 
      ASTVRLFTY WPVFLSLKF LIF
    CDS sequence
    • >TC031189 RA
      atgaaagac tcaaaatta gtgttgaag gaggcacga 
      gatgccatc aaaaacaag aattatgaa actgccttg 
      aaactatgc aagcaaata ctgaaagaa gaaaagaac 
      aattacatg gcccttgtc ttcctggga gtctcatta 
      caggaaacc ggtcaaaac gccaaagct ttgaaagct 
      ttccaggag gcaatcgaa gcaaaccct tcaaatccc 
      ttggcttgg aacggtctt ataaattat tacgaaaaa 
      atcgacact aaagaaact aaacttgat ttaattaat 
      gcttacatt tcgctaatt ggcattgaa acaggcgag 
      aaaaaaatc ctcgaatgt gtccaaaaa ctggcaaat 
      ttgtgcgaa ttcggcgac ttggctaaa atcgtaaca 
      gcgattttt tcagtcctg aaagagggc aagtgtgac 
      agtgcggaa tgtgcagtt tgtttgatt aatatttta 
      tcaaaaatt gaaaatctc gcaccggat ttggtccca 
      atttacgaa gaaacgtta aaactcgcc ttaaacaac 
      caaaaaacc gtatgtacg aaatatttc agcgattat 
      ttaaacctg ttgtataag caagacaaa gcgggtgaa 
      ttattacaa caggcgaaa aaaatgcac gaaatgttt 
      gaatcagac tggatttct ttgcgttgg atttgtaag 
      attttcaat gaggtttac gtcgagtcc ggcactttg 
      atcgaatcg gtcgaaaac atggccaat tacagcgaa 
      agtctctct aaattggag gcaccttca gctacgggt 
      ctattcacc caaagcatt ttactagtt gaacagaaa 
      aaaattatc gaagcgaaa gatgtttta gagcaagtt 
      gtgggggtt ttacccggg ttggtacac gcgtgggta 
      cttttgcta aaatgttgt atgcaactc ctgttgtac 
      gacgaagcc ataaatgcg gcaaacaaa ctcgagaaa 
      ctgttacaa aataagaag attttgaag aaaagtgtt 
      aatgttttg atggttgag gttttgtcc cggtcggag 
      gaggaggcc gaccttcgc aaagcgtta caattattt 
      gaaaatatt gaaactgag gtgaaatct gcgtgcaat 
      caacacttg attcgtgca aacatcaat ctaaaaaaa 
      ttcaccgaa gctaaatcg ttactccaa gaattcaat 
      tctactgat aaaacacac attttgctc aaagcccag 
      ttgctccac aagcaggaa aaattttcc gaagctctg 
      gaaattcta gaaagggaa aattgcgag tcttgtgaa 
      tggtggctc gaaatcggt tcgctttat tgggattgt 
      ggccacttt gacaaaagt ctgatccca tttttgaag 
      gccaccaaa ttggaccca aataactac aagtgtttc 
      ctgtttttg ggccatttc tacaagaga aacaacgac 
      tttgataaa gctagacga tgttacgag aaagctttc 
      aaaattaat aagcgatgt gcggaggcc gggatcgaa 
      ctaagcaaa atctaccgc aagttaaaa aattgggac 
      gccaacttg aacttgtta caagccctc acaagtgga 
      ataattaac gctaaaaat tcctgggct tggatgcag 
      ctagggcta caccacttg gaacaatta gattacgat 
      aaagccgtt acgaacttg cgattcgtc atacgaatt 
      gacgaaaac aatgctcac tgctgggaa tccctggct 
      gacgcctac ttggccaaa ggtgcctac acttccgct 
      ttgaaatgt tacgagaaa acgtcgagt ttggtgtcg 
      gaagcgtgg tatccgatg ttacaaaaa gggcacatt 
      aagcagatt ttgggcgaa tttgccgaa gcgcggctc 
      gagtttgag gaaatttta aaatttaac aagttttac 
      gtcccggct ttgaaaggc atggctgag acttgtctg 
      cttcaagcg aggcattgt tacaaggat cagagactg 
      gggacgagt cgggaccat gctcaggtg gccaccgat 
      cacttgatt cgggctctg agccaacgt ggcgacctc 
      tcgtgtttg tggaaattg ttagggaac gggtgtttg 
      ttcgtggcc aatttacct gaaaaacat tcgtgtctt 
      ttgattgcg aaaaagttc gttgaaggg agggacgtt 
      gtgggtgat acggttcta gagaaaagt gatttattc 
      gcgttggct gcgcgctgt tttttcaaa agtttgggt 
      ctagttgag gataatttt ttgatttgg cacgatttg 
      gccgtttgt tatttatcg cacgggctt agtagcggt 
      tcaagcgag ttaattact aaagctcaa actattttg 
      caacattgc acggctaat aatccgacg tattggcca 
      cactggaat ttattaggc aatgtagcg atgcatcta 
      aaccctcca aattataaa ttcgcgcaa cattgtttc 
      attaaagcc gttatcgct gatagtcac agtgccgtc 
      tcgtggacg aatttaggc actttgtat tttctcctc 
      ggtgatttt aagctggca aatcaggcg ttttcgcaa 
      gctcaaaga tcggaccct aactacgtt aacagttgg 
      ataggacag gcactttta gccgagact ttggcacac 
      gaagacgct atggatttg tttaggcac agcacccag 
      ttaagggtc cacaaacag ggggctttg gggtacgca 
      cattgggtg tgcaaaacg ttgaaggag tcgccggtg 
      gacaccgtc gtctattcg atacacaac atgcatgcg 
      gtccctgtg gcttgtgat gtaatcacc tggtatacg 
      gaacatgat ccaatgaac gccacagcg tggaacatg 
      ctgggaatt ctcaaagaa cgaatgggt ctcaaactg 
      ggtgctttg gaggctttc aaaaacgcc ctaacttta 
      tccgataaa aaacagaga gacttggcc cgagtcaac 
      tacggccga ttactggcc gctttgggc aaataccag 
      caagcagtc gatagtttt acaaaagtc gaagctgcg 
      actttcaac agcggaagc ggcctagcg ctcgcgctc 
      ttcaaagac aaactttac aaagaatcg tacgaagtg 
      tacgaatcc actttacat tggctgtct gacgattct 
      aatttacag tcgaattta ttggtggct ttggcctca 
      atggcctac atgttcgag gggcctgaa gcggccaaa 
      actctgttg tttcaaagt attcagctt gagaatccg 
      tcaccgtgg gggttttac gcgatttcg tctttgggt 
      ttgctccat gatgatttc aatctgact gacttggtt 
      ttaagtgag ttgaaaaaa attatttcg aggagttgt 
      gggccacat tttgccgtt ttggcgagt tatttccac 
      ttgttacaa ggtaaaaat aagcaaagt gtgtgtgag 
      gtgagcaaa attgtgttt atgtacccg gaccaagcg 
      ccagtttgg gcaactctt tcgtcggtt ttgattcga 
      gtggggaaa gatgccaaa gcggctgcc aattgcgcc 
      caaattgcc atgaaatta ggccaagcg actaataca 
      aacgttact aaaattttg tgtctcgtg gctttgtcg 
      tttcttatc gtaggtgac acggcaaaa gcgctctta 
      atggcccag aaagccatt cattgttac ccgaatttg 
      gccgaaggt tgggcggtt tttatagcg gccttggtg 
      cgctccaaa cgaaccaat cgagtccca gagttttta 
      aaaatcgca cagcagttg aatcctttg cgagtgaag 
      ttcgtcgcc atgttgcaa gtcacgctt tggttgatt 
      tttgccttg atttcgagc ggttttgca caaagttgt 
      aaaactcga ggcgaatgg tgcgccgat acttatcag 
      tgttgtggc ggttgttgt attcgcaac acctgcatt 
      gacacctat caagactgc aggataatt agtgatcca 
      tgtctggaa ttttattgt ccgccagat gaaatgtgc 
      cgtttgtac caacctcgg tgcgaaggt tgcgaaatt 
      gaaaaacgg tgtgtgcct gcaggagcc accgacaca 
      gtcatgttc atggaccca gaaaagttg ccctcagca 
      gcttcaaca gtacggctt ttcacatac tggcctgtt 
      tttctgtca ttaaaattc cttattttt taa
    mRNA sequence
    • >TC031189 RA
      gtaatttaa ttaaacaaa tattttttt taattaaat 
      actaagtgc aaatgattc gttttgtac gcttttggt 
      tattttcga gtctagcta ataagtggc aaaaagtgc 
      atttgtagg ttaggcttt tccaaacaa gttattgtt 
      ttgagtgaa tcatgaaag actcaaaat tagtgttga 
      aggaggcac gagatgcca tcaaaaaca agaattatg 
      aaactgcct tgaaactat gcaagcaaa tactgaaag 
      aagaaaaga acaattaca tggcccttg tcttcctgg 
      gagtctcat tacaggaaa ccggtcaaa acgccaaag 
      ctttgaaag ctttccagg aggcaatcg aagcaaacc 
      cttcaaatc ccttggctt ggaacggtc ttataaatt 
      attacgaaa aaatcgaca ctaaagaaa ctaaacttg 
      atttaatta atgcttaca tttcgctaa ttggcattg 
      aaacaggcg agaaaaaaa tcctcgaat gtgtccaaa 
      aactggcaa atttgtgcg aattcggcg acttggcta 
      aaatcgtaa cagcgattt tttcagtcc tgaaagagg 
      gcaagtgtg acagtgcgg aatgtgcag tttgtttga 
      ttaatattt tatcaaaaa ttgaaaatc tcgcaccgg 
      atttggtcc caatttacg aagaaacgt taaaactcg 
      ccttaaaca accaaaaaa ccgtatgta cgaaatatt 
      tcagcgatt atttaaacc tgttgtata agcaagaca 
      aagcgggtg aattattac aacaggcga aaaaaatgc 
      acgaaatgt ttgaatcag actggattt ctttgcgtt 
      ggatttgta agattttca atgaggttt acgtcgagt 
      ccggcactt tgatcgaat cggtcgaaa acatggcca 
      attacagcg aaagtctct ctaaattgg aggcacctt 
      cagctacgg gtctattca cccaaagca ttttactag 
      ttgaacaga aaaaaatta tcgaagcga aagatgttt 
      tagagcaag ttgtggggg ttttacccg ggttggtac 
      acgcgtggg tacttttgc taaaatgtt gtatgcaac 
      tcctgttgt acgacgaag ccataaatg cggcaaaca 
      aactcgaga aactgttac aaaataaga agattttga 
      agaaaagtg ttaatgttt tgatggttg aggttttgt 
      cccggtcgg aggaggagg ccgaccttc gcaaagcgt 
      tacaattat ttgaaaata ttgaaactg aggtgaaat 
      ctgcgtgca atcaacact tgattcgtg caaacatca 
      atctaaaaa aattcaccg aagctaaat cgttactcc 
      aagaattca attctactg ataaaacac acattttgc 
      tcaaagccc agttgctcc acaagcagg aaaaatttt 
      ccgaagctc tggaaattc tagaaaggg aaaattgcg 
      agtcttgtg aatggtggc tcgaaatcg gttcgcttt 
      attgggatt gtggccact ttgacaaaa gtctgatcc 
      catttttga aggccacca aattggacc caaataact 
      acaagtgtt tcctgtttt tgggccatt tctacaaga 
      gaaacaacg actttgata aagctagac gatgttacg 
      agaaagctt tcaaaatta ataagcgat gtgcggagg 
      ccgggatcg aactaagca aaatctacc gcaagttaa 
      aaaattggg acgccaact tgaacttgt tacaagccc 
      tcacaagtg gaataatta acgctaaaa attcctggg 
      cttggatgc agctagggc tacaccact tggaacaat 
      tagattacg ataaagccg ttacgaact tgcgattcg 
      tcatacgaa ttgacgaaa acaatgctc actgctggg 
      aatccctgg ctgacgcct acttggcca aaggtgcct 
      acacttccg ctttgaaat gttacgaga aaacgtcga 
      gtttggtgt cggaagcgt ggtatccga tgttacaaa 
      aagggcaca ttaagcaga ttttgggcg aatttgccg 
      aagcgcggc tcgagtttg aggaaattt taaaattta 
      acaagtttt acgtcccgg ctttgaaag gcatggctg 
      agacttgtc tgcttcaag cgaggcatt gttacaagg 
      atcagagac tggggacga gtcgggacc atgctcagg 
      tggccaccg atcacttga ttcgggctc tgagccaac 
      gtggcgacc tctcgtgtt tgtggaaat tgttaggga 
      acgggtgtt tgttcgtgg ccaatttac ctgaaaaac 
      attcgtgtc ttttgattg cgaaaaagt tcgttgaag 
      ggagggacg ttgtgggtg atacggttc tagagaaaa 
      gtgatttat tcgcgttgg ctgcgcgct gttttttca 
      aaagtttgg gtctagttg aggataatt ttttgattt 
      ggcacgatt tggccgttt gttatttat cgcacgggc 
      ttagtagcg gttcaagcg agttaatta ctaaagctc 
      aaactattt tgcaacatt gcacggcta ataatccga 
      cgtattggc cacactgga atttattag gcaatgtag 
      cgatgcatc taaaccctc caaattata aattcgcgc 
      aacattgtt tcattaaag ccgttatcg ctgatagtc 
      acagtgccg tctcgtgga cgaatttag gcactttgt 
      attttctcc tcggtgatt ttaagctgg caaatcagg 
      cgttttcgc aagctcaaa gatcggacc ctaactacg 
      ttaacagtt ggataggac aggcacttt tagccgaga 
      ctttggcac acgaagacg ctatggatt tgtttaggc 
      acagcaccc agttaaggg tccacaaac agggggctt 
      tggggtacg cacattggg tgtgcaaaa cgttgaagg 
      agtcgccgg tggacaccg tcgtctatt cgatacaca 
      acatgcatg cggtccctg tggcttgtg atgtaatca 
      cctggtata cggaacatg atccaatga acgccacag 
      cgtggaaca tgctgggaa ttctcaaag aacgaatgg 
      gtctcaaac tgggtgctt tggaggctt tcaaaaacg 
      ccctaactt tatccgata aaaaacaga gagacttgg 
      cccgagtca actacggcc gattactgg ccgctttgg 
      gcaaatacc agcaagcag tcgatagtt ttacaaaag 
      tcgaagctg cgactttca acagcggaa gcggcctag 
      cgctcgcgc tcttcaaag acaaacttt acaaagaat 
      cgtacgaag tgtacgaat ccactttac attggctgt 
      ctgacgatt ctaatttac agtcgaatt tattggtgg 
      ctttggcct caatggcct acatgttcg aggggcctg 
      aagcggcca aaactctgt tgtttcaaa gtattcagc 
      ttgagaatc cgtcaccgt gggggtttt acgcgattt 
      cgtctttgg gtttgctcc atgatgatt tcaatctga 
      ctgacttgg ttttaagtg agttgaaaa aaattattt 
      cgaggagtt gtgggccac attttgccg ttttggcga 
      gttatttcc acttgttac aaggtaaaa ataagcaaa 
      gtgtgtgtg aggtgagca aaattgtgt ttatgtacc 
      cggaccaag cgccagttt gggcaactc tttcgtcgg 
      ttttgattc gagtgggga aagatgcca aagcggctg 
      ccaattgcg cccaaattg ccatgaaat taggccaag 
      cgactaata caaacgtta ctaaaattt tgtgtctcg 
      tggctttgt cgtttctta tcgtaggtg acacggcaa 
      aagcgctct taatggccc agaaagcca ttcattgtt 
      acccgaatt tggccgaag gttgggcgg tttttatag 
      cggccttgg tgcgctcca aacgaacca atcgagtcc 
      cagagtttt taaaaatcg cacagcagt tgaatcctt 
      tgcgagtga agttcgtcg ccatgttgc aagtcacgc 
      tttggttga tttttgcct tgatttcga gcggttttg 
      cacaaagtt gtaaaactc gaggcgaat ggtgcgccg 
      atacttatc agtgttgtg gcggttgtt gtattcgca 
      acacctgca ttgacacct atcaagact gcaggataa 
      ttagtgatc catgtctgg aattttatt gtccgccag 
      atgaaatgt gccgtttgt accaacctc ggtgcgaag 
      gttgcgaaa ttgaaaaac ggtgtgtgc ctgcaggag 
      ccaccgaca cagtcatgt tcatggacc cagaaaagt 
      tgccctcag cagcttcaa cagtacggc ttttcacat 
      actggcctg tttttctgt cattaaaat tccttattt 
      tttaaatga tttaattaa atatagttg acttgattt 
      g
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