Status
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Gene information
General information
TC009145 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3834300..3837304
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009145
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01479

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009145 RA
      MADLFAKVS CTYCEEEIT GIRVQCCVC QDFDICLQC 
      FSTGAEIGT HKNDHAYKF VEHWSVSIF GGKGNWTGG 
      EELQLLDAV ELYGFGNWE LVSQHVETR TPEEVRDEY 
      ISRYLDGNI GKATWAESR DRQPMLIDH VPEDNGPLS 
      PAVIARLPP LDITLEEAQ LLGYKPHRD DFEREYDMP 
      AEQLVSTLQ LDTAEDTEV EVALKLSMV DMYTRRLRE 
      RAKRKRIVR DYQLVAKFF SNQRKDSNK RPLTKEQRE 
      LRENMKVFS QFLTSNEHE QLINNLERE KELRHRLSE 
      LYRYRSLGL TTQEEVIHQ EQHMAYQKQ QQMKQSKSG 
      SSGYQISVP EVQSKNGKS EENQNLSEK KFTWSEEQN 
      NSNSDNVRP PSPNALPLG SLLSQHEIQ LCKTLNLQP 
      VQYTTLKSL IIQENLLSP NRHRTVEID KNSSEVSVK 
      DVVTQYLSH SGWLPGVSV V
    • >TC009145 RB
      MADLFAKVS CTYCEEEIT GIRVQCCVC QDFDICLQC 
      FSTGAEIGT HKNDHAYKF VEHWSVSIF GGKGNWTGG 
      EELQLLDAV ELYGFGNWE LVSQHVETR TPEEVRDEY 
      ISRYLDGNI GKATWAESR DRQPMLIDH VPEDNGPLS 
      PAVIARLPP LDITLEEAQ LLGYKPHRD DFEREYDMP 
      AEQLVSTLQ LDTAEDTEV EVALKLSMV DMYTRRLRE 
      RAKRKRIVR DYQLVAKFF SNQRKDSNK RPLTKEQRE 
      LRENMKVFS QFLTSNEHE QLINNLERE KELRHRLSE 
      LYRYRSLGL TTQEEVIHQ EQHMAYQKQ QQMKQSKSA 
      RLKRNKYGS SGYQISVPE VQSKNGKSE ENQNLSEKK 
      FTWSEEQNN SNSDNVRPP SPNALPLGS LLSQHEIQL 
      CKTLNLQPV QYTTLKSLI IQENLLSPN RHRTVEIDK 
      NSSEVSVKD VVTQYLSHS GWLPGVSVV
    CDS sequence
    • >TC009145 RA
      atggctgat ctctttgca aaagtctct tgtacttat 
      tgcgaggaa gaaatcacg ggaattcgg gtccaatgc 
      tgcgtttgc caggacttc gacatttgc ttgcagtgt 
      ttttcaacg ggggctgaa atcgggacg cataaaaac 
      gatcatgcg tataaattt gtggaacac tggtcggtt 
      agtattttt gggggaaag gggaattgg actggaggt 
      gaggaattg caattgttg gatgctgtg gagttgtat 
      gggtttggg aattgggag ttggtgagc caacatgtt 
      gaaacacgg acccctgaa gaagtgaga gatgaatat 
      atttcgcgt tatttggac ggtaatatt ggaaaagcc 
      acttgggct gaatcgaga gaccgtcaa ccgatgttg 
      attgatcat gtacctgaa gataatgga ccattatcg 
      cctgcagtg attgcaagg ttgccacct ttagatata 
      actctagaa gaagctcaa ttgttaggg tataaacca 
      catagggac gattttgaa cgggaatat gatatgcca 
      gctgaacaa ctggtttca actttacaa ttagacaca 
      gctgaagac accgaagtc gaagtagcg ctaaaatta 
      tcaatggtt gatatgtac actaggaga ttaagagaa 
      agagcgaaa cgaaaacga atcgttcgc gactaccaa 
      ttggttgca aaatttttc tcaaaccaa agaaaagat 
      tcaaataag agacctcta acaaaagag caaagagaa 
      cttagggaa aacatgaaa gtctttagt cagttttta 
      acatcaaac gaacatgaa caactaatc aataacctt 
      gaaagagag aaagagtta cgtcaccgt ttatccgaa 
      ttgtataga tatagaagt ttaggttta acaacacaa 
      gaggaagtg atacatcaa gaacaacac atggcgtat 
      cagaaacaa caacaaatg aaacagagc aagtctggc 
      agcagtggc tatcaaata tcagttccg gaggtgcag 
      tcaaaaaac ggaaaaagc gaagaaaac caaaattta 
      tccgaaaaa aaattcacg tggtccgaa gagcaaaac 
      aactcgaac tcggataat gtaagaccg ccgtcacct 
      aatgcctta ccgttagga tccttattg tcccaacat 
      gaaatacag ctttgcaag actttaaat ttgcaaccc 
      gtccaatac accacactg aaatcgctt attattcag 
      gaaaatctc ttgtctccg aatcgacac cgtaccgtt 
      gaaattgat aaaaattcg tctgaagtt agtgttaaa 
      gatgttgtt acgcaatat cttagtcac agtggctgg 
      cttccaggg gtttctgtt gtctga
    • >TC009145 RB
      atggctgat ctctttgca aaagtctct tgtacttat 
      tgcgaggaa gaaatcacg ggaattcgg gtccaatgc 
      tgcgtttgc caggacttc gacatttgc ttgcagtgt 
      ttttcaacg ggggctgaa atcgggacg cataaaaac 
      gatcatgcg tataaattt gtggaacac tggtcggtt 
      agtattttt gggggaaag gggaattgg actggaggt 
      gaggaattg caattgttg gatgctgtg gagttgtat 
      gggtttggg aattgggag ttggtgagc caacatgtt 
      gaaacacgg acccctgaa gaagtgaga gatgaatat 
      atttcgcgt tatttggac ggtaatatt ggaaaagcc 
      acttgggct gaatcgaga gaccgtcaa ccgatgttg 
      attgatcat gtacctgaa gataatgga ccattatcg 
      cctgcagtg attgcaagg ttgccacct ttagatata 
      actctagaa gaagctcaa ttgttaggg tataaacca 
      catagggac gattttgaa cgggaatat gatatgcca 
      gctgaacaa ctggtttca actttacaa ttagacaca 
      gctgaagac accgaagtc gaagtagcg ctaaaatta 
      tcaatggtt gatatgtac actaggaga ttaagagaa 
      agagcgaaa cgaaaacga atcgttcgc gactaccaa 
      ttggttgca aaatttttc tcaaaccaa agaaaagat 
      tcaaataag agacctcta acaaaagag caaagagaa 
      cttagggaa aacatgaaa gtctttagt cagttttta 
      acatcaaac gaacatgaa caactaatc aataacctt 
      gaaagagag aaagagtta cgtcaccgt ttatccgaa 
      ttgtataga tatagaagt ttaggttta acaacacaa 
      gaggaagtg atacatcaa gaacaacac atggcgtat 
      cagaaacaa caacaaatg aaacagagc aagtctgct 
      agactcaaa cgaaacaaa tatggcagc agtggctat 
      caaatatca gttccggag gtgcagtca aaaaacgga 
      aaaagcgaa gaaaaccaa aatttatcc gaaaaaaaa 
      ttcacgtgg tccgaagag caaaacaac tcgaactcg 
      gataatgta agaccgccg tcacctaat gccttaccg 
      ttaggatcc ttattgtcc caacatgaa atacagctt 
      tgcaagact ttaaatttg caacccgtc caatacacc 
      acactgaaa tcgcttatt attcaggaa aatctcttg 
      tctccgaat cgacaccgt accgttgaa attgataaa 
      aattcgtct gaagttagt gttaaagat gttgttacg 
      caatatctt agtcacagt ggctggctt ccaggggtt 
      tctgttgtc tga
    mRNA sequence
    • >TC009145 RA
      agctagatg accccaact cgttttgag taataaaat 
      cgataaaaa agtgttgga attgaaatt aatttggga 
      aaaatcaat ttggaaaat gtataagcc ggccctgtg 
      gcgcggcgc gcaggcgca aattgatcg cttcgctta 
      cacagcact ccacgttca ttccaattt gttttttct 
      ttcactcaa aaaaacgga tttgcgccg catctcgtg 
      cctgtgttt cccccaatc gtccaaatg aaccgatct 
      gtggaccac cgtgtcgtt tagaatgcc ccagagccc 
      gtgaattgc atttctggc attgccccc caccactgg 
      tcattttga cgtcgcaag aaaatggct gatctcttt 
      gcaaaagtc tcttgtact tattgcgag gaagaaatc 
      acgggaatt cgggtccaa tgctgcgtt tgccaggac 
      ttcgacatt tgcttgcag tgtttttca acgggggct 
      gaaatcggg acgcataaa aacgatcat gcgtataaa 
      tttgtggaa cactggtcg gttagtatt tttggggga 
      aaggggaat tggactgga ggtgaggaa ttgcaattg 
      ttggatgct gtggagttg tatgggttt gggaattgg 
      gagttggtg agccaacat gttgaaaca cggacccct 
      gaagaagtg agagatgaa tatatttcg cgttatttg 
      gacggtaat attggaaaa gccacttgg gctgaatcg 
      agagaccgt caaccgatg ttgattgat catgtacct 
      gaagataat ggaccatta tcgcctgca gtgattgca 
      aggttgcca cctttagat ataactcta gaagaagct 
      caattgtta gggtataaa ccacatagg gacgatttt 
      gaacgggaa tatgatatg ccagctgaa caactggtt 
      tcaacttta caattagac acagctgaa gacaccgaa 
      gtcgaagta gcgctaaaa ttatcaatg gttgatatg 
      tacactagg agattaaga gaaagagcg aaacgaaaa 
      cgaatcgtt cgcgactac caattggtt gcaaaattt 
      ttctcaaac caaagaaaa gattcaaat aagagacct 
      ctaacaaaa gagcaaaga gaacttagg gaaaacatg 
      aaagtcttt agtcagttt ttaacatca aacgaacat 
      gaacaacta atcaataac cttgaaaga gagaaagag 
      ttacgtcac cgtttatcc gaattgtat agatataga 
      agtttaggt ttaacaaca caagaggaa gtgatacat 
      caagaacaa cacatggcg tatcagaaa caacaacaa 
      atgaaacag agcaagtct ggcagcagt ggctatcaa 
      atatcagtt ccggaggtg cagtcaaaa aacggaaaa 
      agcgaagaa aaccaaaat ttatccgaa aaaaaattc 
      acgtggtcc gaagagcaa aacaactcg aactcggat 
      aatgtaaga ccgccgtca cctaatgcc ttaccgtta 
      ggatcctta ttgtcccaa catgaaata cagctttgc 
      aagacttta aatttgcaa cccgtccaa tacaccaca 
      ctgaaatcg cttattatt caggaaaat ctcttgtct 
      ccgaatcga caccgtacc gttgaaatt gataaaaat 
      tcgtctgaa gttagtgtt aaagatgtt gttacgcaa 
      tatcttagt cacagtggc tggcttcca ggggtttct 
      gttgtctga tctttgatt atgtaaaat tggaattaa 
      tttttgttt gtcacgata ttttagtta gctgggtta 
      acactgatg ggttagtgt cttttttag tgtaatcga 
      cacttaaaa attgttgta gtggcataa ttgtatgtt 
      cctttatca tgtattcg
    • >TC009145 RB
      agctagatg accccaact cgttttgag taataaaat 
      cgataaaaa agtgttgga attgaaatt aatttggga 
      aaaatcaat ttggaaaat gtataagcc ggccctgtg 
      gcgcggcgc gcaggcgca aattgatcg cttcgctta 
      cacagcact ccacgttca ttccaattt gttttttct 
      ttcactcaa aaaaacgga tttgcgccg catctcgtg 
      cctgtgttt cccccaatc gtccaaatg aaccgatct 
      gtggaccac cgtgtcgtt tagaatgcc ccagagccc 
      gtgaattgc atttctggc attgccccc caccactgg 
      tcattttga cgtcgcaag aaaatggct gatctcttt 
      gcaaaagtc tcttgtact tattgcgag gaagaaatc 
      acgggaatt cgggtccaa tgctgcgtt tgccaggac 
      ttcgacatt tgcttgcag tgtttttca acgggggct 
      gaaatcggg acgcataaa aacgatcat gcgtataaa 
      tttgtggaa cactggtcg gttagtatt tttggggga 
      aaggggaat tggactgga ggtgaggaa ttgcaattg 
      ttggatgct gtggagttg tatgggttt gggaattgg 
      gagttggtg agccaacat gttgaaaca cggacccct 
      gaagaagtg agagatgaa tatatttcg cgttatttg 
      gacggtaat attggaaaa gccacttgg gctgaatcg 
      agagaccgt caaccgatg ttgattgat catgtacct 
      gaagataat ggaccatta tcgcctgca gtgattgca 
      aggttgcca cctttagat ataactcta gaagaagct 
      caattgtta gggtataaa ccacatagg gacgatttt 
      gaacgggaa tatgatatg ccagctgaa caactggtt 
      tcaacttta caattagac acagctgaa gacaccgaa 
      gtcgaagta gcgctaaaa ttatcaatg gttgatatg 
      tacactagg agattaaga gaaagagcg aaacgaaaa 
      cgaatcgtt cgcgactac caattggtt gcaaaattt 
      ttctcaaac caaagaaaa gattcaaat aagagacct 
      ctaacaaaa gagcaaaga gaacttagg gaaaacatg 
      aaagtcttt agtcagttt ttaacatca aacgaacat 
      gaacaacta atcaataac cttgaaaga gagaaagag 
      ttacgtcac cgtttatcc gaattgtat agatataga 
      agtttaggt ttaacaaca caagaggaa gtgatacat 
      caagaacaa cacatggcg tatcagaaa caacaacaa 
      atgaaacag agcaagtct gctagactc aaacgaaac 
      aaatatggc agcagtggc tatcaaata tcagttccg 
      gaggtgcag tcaaaaaac ggaaaaagc gaagaaaac 
      caaaattta tccgaaaaa aaattcacg tggtccgaa 
      gagcaaaac aactcgaac tcggataat gtaagaccg 
      ccgtcacct aatgcctta ccgttagga tccttattg 
      tcccaacat gaaatacag ctttgcaag actttaaat 
      ttgcaaccc gtccaatac accacactg aaatcgctt 
      attattcag gaaaatctc ttgtctccg aatcgacac 
      cgtaccgtt gaaattgat aaaaattcg tctgaagtt 
      agtgttaaa gatgttgtt acgcaatat cttagtcac 
      agtggctgg cttccaggg gtttctgtt gtctgatct 
      ttgattatg taaaattgg aattaattt ttgtttgtc 
      acgatattt tagttagct gggttaaca ctgatgggt 
      tagtgtctt ttttagtgt aatcgacac ttaaaaatt 
      gttgtagtg gcataattg tatgttcct ttatcatgt 
      attcg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY