Status
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Gene information
General information
TC009145 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3834300..3837304
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009145
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01479

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009145 RA
      MADLFAKVS CTYCEEEIT GIRVQCCVC QDFDICLQC 
FSTGAEIGT HKNDHAYKF VEHWSVSIF GGKGNWTGG 
EELQLLDAV ELYGFGNWE LVSQHVETR TPEEVRDEY 
ISRYLDGNI GKATWAESR DRQPMLIDH VPEDNGPLS 
PAVIARLPP LDITLEEAQ LLGYKPHRD DFEREYDMP 
AEQLVSTLQ LDTAEDTEV EVALKLSMV DMYTRRLRE 
RAKRKRIVR DYQLVAKFF SNQRKDSNK RPLTKEQRE 
LRENMKVFS QFLTSNEHE QLINNLERE KELRHRLSE 
LYRYRSLGL TTQEEVIHQ EQHMAYQKQ QQMKQSKSG 
SSGYQISVP EVQSKNGKS EENQNLSEK KFTWSEEQN 
NSNSDNVRP PSPNALPLG SLLSQHEIQ LCKTLNLQP 
VQYTTLKSL IIQENLLSP NRHRTVEID KNSSEVSVK 
DVVTQYLSH SGWLPGVSV V
    • >TC009145 RB
      MADLFAKVS CTYCEEEIT GIRVQCCVC QDFDICLQC 
FSTGAEIGT HKNDHAYKF VEHWSVSIF GGKGNWTGG 
EELQLLDAV ELYGFGNWE LVSQHVETR TPEEVRDEY 
ISRYLDGNI GKATWAESR DRQPMLIDH VPEDNGPLS 
PAVIARLPP LDITLEEAQ LLGYKPHRD DFEREYDMP 
AEQLVSTLQ LDTAEDTEV EVALKLSMV DMYTRRLRE 
RAKRKRIVR DYQLVAKFF SNQRKDSNK RPLTKEQRE 
LRENMKVFS QFLTSNEHE QLINNLERE KELRHRLSE 
LYRYRSLGL TTQEEVIHQ EQHMAYQKQ QQMKQSKSA 
RLKRNKYGS SGYQISVPE VQSKNGKSE ENQNLSEKK 
FTWSEEQNN SNSDNVRPP SPNALPLGS LLSQHEIQL 
CKTLNLQPV QYTTLKSLI IQENLLSPN RHRTVEIDK 
NSSEVSVKD VVTQYLSHS GWLPGVSVV
    CDS sequence
    • >TC009145 RA
      atggctgat ctctttgca aaagtctct tgtacttat 
tgcgaggaa gaaatcacg ggaattcgg gtccaatgc 
tgcgtttgc caggacttc gacatttgc ttgcagtgt 
ttttcaacg ggggctgaa atcgggacg cataaaaac 
gatcatgcg tataaattt gtggaacac tggtcggtt 
agtattttt gggggaaag gggaattgg actggaggt 
gaggaattg caattgttg gatgctgtg gagttgtat 
gggtttggg aattgggag ttggtgagc caacatgtt 
gaaacacgg acccctgaa gaagtgaga gatgaatat 
atttcgcgt tatttggac ggtaatatt ggaaaagcc 
acttgggct gaatcgaga gaccgtcaa ccgatgttg 
attgatcat gtacctgaa gataatgga ccattatcg 
cctgcagtg attgcaagg ttgccacct ttagatata 
actctagaa gaagctcaa ttgttaggg tataaacca 
catagggac gattttgaa cgggaatat gatatgcca 
gctgaacaa ctggtttca actttacaa ttagacaca 
gctgaagac accgaagtc gaagtagcg ctaaaatta 
tcaatggtt gatatgtac actaggaga ttaagagaa 
agagcgaaa cgaaaacga atcgttcgc gactaccaa 
ttggttgca aaatttttc tcaaaccaa agaaaagat 
tcaaataag agacctcta acaaaagag caaagagaa 
cttagggaa aacatgaaa gtctttagt cagttttta 
acatcaaac gaacatgaa caactaatc aataacctt 
gaaagagag aaagagtta cgtcaccgt ttatccgaa 
ttgtataga tatagaagt ttaggttta acaacacaa 
gaggaagtg atacatcaa gaacaacac atggcgtat 
cagaaacaa caacaaatg aaacagagc aagtctggc 
agcagtggc tatcaaata tcagttccg gaggtgcag 
tcaaaaaac ggaaaaagc gaagaaaac caaaattta 
tccgaaaaa aaattcacg tggtccgaa gagcaaaac 
aactcgaac tcggataat gtaagaccg ccgtcacct 
aatgcctta ccgttagga tccttattg tcccaacat 
gaaatacag ctttgcaag actttaaat ttgcaaccc 
gtccaatac accacactg aaatcgctt attattcag 
gaaaatctc ttgtctccg aatcgacac cgtaccgtt 
gaaattgat aaaaattcg tctgaagtt agtgttaaa 
gatgttgtt acgcaatat cttagtcac agtggctgg 
cttccaggg gtttctgtt gtctga
    • >TC009145 RB
      atggctgat ctctttgca aaagtctct tgtacttat 
tgcgaggaa gaaatcacg ggaattcgg gtccaatgc 
tgcgtttgc caggacttc gacatttgc ttgcagtgt 
ttttcaacg ggggctgaa atcgggacg cataaaaac 
gatcatgcg tataaattt gtggaacac tggtcggtt 
agtattttt gggggaaag gggaattgg actggaggt 
gaggaattg caattgttg gatgctgtg gagttgtat 
gggtttggg aattgggag ttggtgagc caacatgtt 
gaaacacgg acccctgaa gaagtgaga gatgaatat 
atttcgcgt tatttggac ggtaatatt ggaaaagcc 
acttgggct gaatcgaga gaccgtcaa ccgatgttg 
attgatcat gtacctgaa gataatgga ccattatcg 
cctgcagtg attgcaagg ttgccacct ttagatata 
actctagaa gaagctcaa ttgttaggg tataaacca 
catagggac gattttgaa cgggaatat gatatgcca 
gctgaacaa ctggtttca actttacaa ttagacaca 
gctgaagac accgaagtc gaagtagcg ctaaaatta 
tcaatggtt gatatgtac actaggaga ttaagagaa 
agagcgaaa cgaaaacga atcgttcgc gactaccaa 
ttggttgca aaatttttc tcaaaccaa agaaaagat 
tcaaataag agacctcta acaaaagag caaagagaa 
cttagggaa aacatgaaa gtctttagt cagttttta 
acatcaaac gaacatgaa caactaatc aataacctt 
gaaagagag aaagagtta cgtcaccgt ttatccgaa 
ttgtataga tatagaagt ttaggttta acaacacaa 
gaggaagtg atacatcaa gaacaacac atggcgtat 
cagaaacaa caacaaatg aaacagagc aagtctgct 
agactcaaa cgaaacaaa tatggcagc agtggctat 
caaatatca gttccggag gtgcagtca aaaaacgga 
aaaagcgaa gaaaaccaa aatttatcc gaaaaaaaa 
ttcacgtgg tccgaagag caaaacaac tcgaactcg 
gataatgta agaccgccg tcacctaat gccttaccg 
ttaggatcc ttattgtcc caacatgaa atacagctt 
tgcaagact ttaaatttg caacccgtc caatacacc 
acactgaaa tcgcttatt attcaggaa aatctcttg 
tctccgaat cgacaccgt accgttgaa attgataaa 
aattcgtct gaagttagt gttaaagat gttgttacg 
caatatctt agtcacagt ggctggctt ccaggggtt 
tctgttgtc tga
    mRNA sequence
    • >TC009145 RA
      agctagatg accccaact cgttttgag taataaaat 
cgataaaaa agtgttgga attgaaatt aatttggga 
aaaatcaat ttggaaaat gtataagcc ggccctgtg 
gcgcggcgc gcaggcgca aattgatcg cttcgctta 
cacagcact ccacgttca ttccaattt gttttttct 
ttcactcaa aaaaacgga tttgcgccg catctcgtg 
cctgtgttt cccccaatc gtccaaatg aaccgatct 
gtggaccac cgtgtcgtt tagaatgcc ccagagccc 
gtgaattgc atttctggc attgccccc caccactgg 
tcattttga cgtcgcaag aaaatggct gatctcttt 
gcaaaagtc tcttgtact tattgcgag gaagaaatc 
acgggaatt cgggtccaa tgctgcgtt tgccaggac 
ttcgacatt tgcttgcag tgtttttca acgggggct 
gaaatcggg acgcataaa aacgatcat gcgtataaa 
tttgtggaa cactggtcg gttagtatt tttggggga 
aaggggaat tggactgga ggtgaggaa ttgcaattg 
ttggatgct gtggagttg tatgggttt gggaattgg 
gagttggtg agccaacat gttgaaaca cggacccct 
gaagaagtg agagatgaa tatatttcg cgttatttg 
gacggtaat attggaaaa gccacttgg gctgaatcg 
agagaccgt caaccgatg ttgattgat catgtacct 
gaagataat ggaccatta tcgcctgca gtgattgca 
aggttgcca cctttagat ataactcta gaagaagct 
caattgtta gggtataaa ccacatagg gacgatttt 
gaacgggaa tatgatatg ccagctgaa caactggtt 
tcaacttta caattagac acagctgaa gacaccgaa 
gtcgaagta gcgctaaaa ttatcaatg gttgatatg 
tacactagg agattaaga gaaagagcg aaacgaaaa 
cgaatcgtt cgcgactac caattggtt gcaaaattt 
ttctcaaac caaagaaaa gattcaaat aagagacct 
ctaacaaaa gagcaaaga gaacttagg gaaaacatg 
aaagtcttt agtcagttt ttaacatca aacgaacat 
gaacaacta atcaataac cttgaaaga gagaaagag 
ttacgtcac cgtttatcc gaattgtat agatataga 
agtttaggt ttaacaaca caagaggaa gtgatacat 
caagaacaa cacatggcg tatcagaaa caacaacaa 
atgaaacag agcaagtct ggcagcagt ggctatcaa 
atatcagtt ccggaggtg cagtcaaaa aacggaaaa 
agcgaagaa aaccaaaat ttatccgaa aaaaaattc 
acgtggtcc gaagagcaa aacaactcg aactcggat 
aatgtaaga ccgccgtca cctaatgcc ttaccgtta 
ggatcctta ttgtcccaa catgaaata cagctttgc 
aagacttta aatttgcaa cccgtccaa tacaccaca 
ctgaaatcg cttattatt caggaaaat ctcttgtct 
ccgaatcga caccgtacc gttgaaatt gataaaaat 
tcgtctgaa gttagtgtt aaagatgtt gttacgcaa 
tatcttagt cacagtggc tggcttcca ggggtttct 
gttgtctga tctttgatt atgtaaaat tggaattaa 
tttttgttt gtcacgata ttttagtta gctgggtta 
acactgatg ggttagtgt cttttttag tgtaatcga 
cacttaaaa attgttgta gtggcataa ttgtatgtt 
cctttatca tgtattcg
    • >TC009145 RB
      agctagatg accccaact cgttttgag taataaaat 
cgataaaaa agtgttgga attgaaatt aatttggga 
aaaatcaat ttggaaaat gtataagcc ggccctgtg 
gcgcggcgc gcaggcgca aattgatcg cttcgctta 
cacagcact ccacgttca ttccaattt gttttttct 
ttcactcaa aaaaacgga tttgcgccg catctcgtg 
cctgtgttt cccccaatc gtccaaatg aaccgatct 
gtggaccac cgtgtcgtt tagaatgcc ccagagccc 
gtgaattgc atttctggc attgccccc caccactgg 
tcattttga cgtcgcaag aaaatggct gatctcttt 
gcaaaagtc tcttgtact tattgcgag gaagaaatc 
acgggaatt cgggtccaa tgctgcgtt tgccaggac 
ttcgacatt tgcttgcag tgtttttca acgggggct 
gaaatcggg acgcataaa aacgatcat gcgtataaa 
tttgtggaa cactggtcg gttagtatt tttggggga 
aaggggaat tggactgga ggtgaggaa ttgcaattg 
ttggatgct gtggagttg tatgggttt gggaattgg 
gagttggtg agccaacat gttgaaaca cggacccct 
gaagaagtg agagatgaa tatatttcg cgttatttg 
gacggtaat attggaaaa gccacttgg gctgaatcg 
agagaccgt caaccgatg ttgattgat catgtacct 
gaagataat ggaccatta tcgcctgca gtgattgca 
aggttgcca cctttagat ataactcta gaagaagct 
caattgtta gggtataaa ccacatagg gacgatttt 
gaacgggaa tatgatatg ccagctgaa caactggtt 
tcaacttta caattagac acagctgaa gacaccgaa 
gtcgaagta gcgctaaaa ttatcaatg gttgatatg 
tacactagg agattaaga gaaagagcg aaacgaaaa 
cgaatcgtt cgcgactac caattggtt gcaaaattt 
ttctcaaac caaagaaaa gattcaaat aagagacct 
ctaacaaaa gagcaaaga gaacttagg gaaaacatg 
aaagtcttt agtcagttt ttaacatca aacgaacat 
gaacaacta atcaataac cttgaaaga gagaaagag 
ttacgtcac cgtttatcc gaattgtat agatataga 
agtttaggt ttaacaaca caagaggaa gtgatacat 
caagaacaa cacatggcg tatcagaaa caacaacaa 
atgaaacag agcaagtct gctagactc aaacgaaac 
aaatatggc agcagtggc tatcaaata tcagttccg 
gaggtgcag tcaaaaaac ggaaaaagc gaagaaaac 
caaaattta tccgaaaaa aaattcacg tggtccgaa 
gagcaaaac aactcgaac tcggataat gtaagaccg 
ccgtcacct aatgcctta ccgttagga tccttattg 
tcccaacat gaaatacag ctttgcaag actttaaat 
ttgcaaccc gtccaatac accacactg aaatcgctt 
attattcag gaaaatctc ttgtctccg aatcgacac 
cgtaccgtt gaaattgat aaaaattcg tctgaagtt 
agtgttaaa gatgttgtt acgcaatat cttagtcac 
agtggctgg cttccaggg gtttctgtt gtctgatct 
ttgattatg taaaattgg aattaattt ttgtttgtc 
acgatattt tagttagct gggttaaca ctgatgggt 
tagtgtctt ttttagtgt aatcgacac ttaaaaatt 
gttgtagtg gcataattg tatgttcct ttatcatgt 
attcg

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY