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Gene information
General information
TC033631
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009132
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 2573840..2577707
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033631
    iBeetle screen iB_01477
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 4
    previtellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4
    mature egg not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (19 dpi)
    Days post injection


    remarks: animals lost, pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (22 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 7 (41 dpi)
    Days post injection


    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033631 RA
      MSSNLSCAV CGTKTNLLR CARCKTRIY CSKEHQRKD 
      WPNHRRECA QLALALDKQ SPKSGESSI LNSLSEDLS 
      PHLDFDKTS TEKALKSAK TAMPISGEN IIHPKKSVV 
      KDFPEISFQ QNPWSAKGH RKDLDEICR NVIGDMDAY 
      GVCVVDNFL GEDKGKAVL KEVLQLYDK GVFRDGQLV 
      SSRGSGDLK TIRGDQIIW IDGKEKSCN NIQQLISQV 
      DAVVMRANR MTNNGKMGM YNINGRTKA MVACYPGLG 
      SHYVKHVDN PNQDGRCIT AIYYLNLNW NVRENGGLL 
      RIFPEGWLG DKVADIEPI FDRLLFFWS DRRNPHEVQ 
      PAYDTRYAI TLWYFDAKE RAEACRRYE KERHS
    CDS sequence
    • >TC033631 RA
      atgagttca aatttaagt tgcgcggtg tgcggcacg 
      aagacaaat ttgctccgc tgtgctcgt tgcaaaacg 
      cgaatttac tgttccaaa gaacaccag cgcaaagac 
      tggcccaac caccgcaga gagtgcgcc caactagcc 
      ttagccctc gacaaacag tcaccgaaa agcggtgaa 
      agcagcatc ctgaactcg ctgtcggaa gacctgagc 
      ccccacttg gacttcgac aagacttcc acggagaaa 
      gccttaaag tcggccaag acagccatg cccataagt 
      ggcgagaac atcatccac cccaaaaag tcagtggtg 
      aaggacttc ccggaaatc tccttccag cagaacccc 
      tggagcgcc aagggccac cgcaaggac ctggacgag 
      atctgcagg aatgtgatc ggggacatg gacgcgtac 
      ggggtctgt gtggtggac aacttcctg ggggaggac 
      aagggcaag gcggtgctg aaggaggtc ttgcagctg 
      tacgacaag ggggttttc cgggacggg cagctggtt 
      tccagcagg ggctcgggc gacttgaag acgatacga 
      ggggaccaa attatctgg atcgatggg aaggaaaaa 
      tcttgcaat aacattcaa cagttgatc agtcaggtt 
      gatgcggtg gtcatgagg gccaacaga atgaccaac 
      aatggcaaa atgggaatg tacaatatt aacggccgg 
      actaaggcg atggtggcc tgttacccc ggtttggga 
      tctcactac gtcaaacac gtggacaac ccaaaccaa 
      gacggtcgt tgtataaca gccatttac tacctcaat 
      ttaaattgg aatgttagg gaaaacggg ggtttatta 
      cgaattttc ccagaggga tggttaggt gataaggta 
      gctgatata gagccaatt ttcgaccgt cttttattt 
      ttctggtct gaccggaga aatccacat gaagtccaa 
      cccgcctac gacacaaga tatgcaata acgttgtgg 
      tatttcgac gctaaagaa cgagcagaa gcatgccgg 
      agatacgaa aaagaacgg cattcatag
    mRNA sequence
    • >TC033631 RA
      aacaacaat ggcggccgt gtcttacag cggtcttga 
      aatttaaag atgagttca aatttaagt tgcgcggtg 
      tgcggcacg aagacaaat ttgctccgc tgtgctcgt 
      tgcaaaacg cgaatttac tgttccaaa gaacaccag 
      cgcaaagac tggcccaac caccgcaga gagtgcgcc 
      caactagcc ttagccctc gacaaacag tcaccgaaa 
      agcggtgaa agcagcatc ctgaactcg ctgtcggaa 
      gacctgagc ccccacttg gacttcgac aagacttcc 
      acggagaaa gccttaaag tcggccaag acagccatg 
      cccataagt ggcgagaac atcatccac cccaaaaag 
      tcagtggtg aaggacttc ccggaaatc tccttccag 
      cagaacccc tggagcgcc aagggccac cgcaaggac 
      ctggacgag atctgcagg aatgtgatc ggggacatg 
      gacgcgtac ggggtctgt gtggtggac aacttcctg 
      ggggaggac aagggcaag gcggtgctg aaggaggtc 
      ttgcagctg tacgacaag ggggttttc cgggacggg 
      cagctggtt tccagcagg ggctcgggc gacttgaag 
      acgatacga ggggaccaa attatctgg atcgatggg 
      aaggaaaaa tcttgcaat aacattcaa cagttgatc 
      agtcaggtt gatgcggtg gtcatgagg gccaacaga 
      atgaccaac aatggcaaa atgggaatg tacaatatt 
      aacggccgg actaaggcg atggtggcc tgttacccc 
      ggtttggga tctcactac gtcaaacac gtggacaac 
      ccaaaccaa gacggtcgt tgtataaca gccatttac 
      tacctcaat ttaaattgg aatgttagg gaaaacggg 
      ggtttatta cgaattttc ccagaggga tggttaggt 
      gataaggta gctgatata gagccaatt ttcgaccgt 
      cttttattt ttctggtct gaccggaga aatccacat 
      gaagtccaa cccgcctac gacacaaga tatgcaata 
      acgttgtgg tatttcgac gctaaagaa cgagcagaa 
      gcatgccgg agatacgaa aaagaacgg cattcatag 
      cgctcgaaa attgtatcg tgccactaa cgatcaact 
      gtgtccgtt ttgtaacaa acaaaccta gtactatgt 
      gatatttat cgtttgttg aaaactggt aacagttgt 
      gtactgttg tatataatg tgtattttt ctgaaagct 
      aaacttttt ttgattatt tatgtttga cagtgtgat 
      attttctg