Status
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Gene information
General information
TC009118 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4803036..4812174
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009118
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01476

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 leg musculature sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: body wall musculature without detectable defect
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009118 RA
      MLWILLFLL SLGSIQALP QGAPTKVCQ TMKPFHGGG 
      IPPQQDIAP YSIYMRRFN GGVLVTLIS DLGIPFQGF 
      MLQARTPNR ELLGVFQPR GNGSHTIDC AQSGDTLTH 
      NDANNKAMV EVEWHPPEG YEGPVVFNT TIAQNYQTF 
      WVGVESRTL KVSKGFTDD PISNIPSRR PSSSTPPSF 
      SPEIREVET VFDPFYEGC GVTKLCFGA PANCINSKN 
      CKAVVGVIV SGDKYEFEM KATGNAGWV GVGLSDDDK 
      MGDDSVIEC VKRGSNVAA FMSWTSGPP SYGAARLNN 
      PQLGIKLVN ASIINDVLY CKVMRDERT KVRGKIFDL 
      ANEKYHILV ASGSQVSPN SVAFHDLAF LASGNKQSL 
      SDVSALAAA SKLLIRLHG SFMLAAWIG TVSVGILLA 
      RYYRNTWVG SSLCGKDLW FAWHRMFMV LTWALTVTG 
      FVLIFVELR AWSAEKNPH AILGTVTTI ICFIQPIGA 
      YFRPHPGTP KRPVFNWIH WLGGNVAHI IGIVTIFFA 
      VKLTKAELP DFVDWILVA YVAFHVIIH LILSVVGCI 
      SEKSFERRV TSFPMKDLG GSGRSSAYA DRSGDAPYS 
      GFRKFILAV YIVIVLAIV VALIVITAL APIEESWNN 
      LRNSMMNAK
    CDS sequence
    • >TC009118 RA
      atgctctgg atcctcctc ttcctcctt tccctcggc 
      tcgatccag gcccttccc caaggcgcc ccgaccaag 
      gtatgccag accatgaag ccgttccac ggaggaggc 
      atccctcct cagcaggac attgctcca tactcaatc 
      tacatgcgt cggttcaac ggtggcgtt ctcgtgacg 
      ctcatatcc gacctggga atcccattc caaggtttc 
      atgctccag gccaggacg cccaaccga gagctcctg 
      ggagtgttc cagccccga ggaaacggc agtcacacc 
      atcgactgc gcgcagtct ggggacacc ctcacccac 
      aacgacgcc aacaacaag gcgatggtg gaggtcgag 
      tggcacccg ccagagggg tacgagggc ccagtggtc 
      ttcaacacg accattgcg caaaattac caaaccttc 
      tgggtcggg gtggagtcc cggactttg aaggtgagt 
      aaggggttc acagacgac cccatttcg aacatcccg 
      agtcggaga ccatcgtcc tccacaccg cccagtttt 
      tcccccgag attagggaa gtggagact gtgtttgat 
      cccttctat gaaggctgc ggtgtcacc aagctgtgt 
      tttggcgca ccggccaat tgcatcaac tcgaagaat 
      tgcaaagcc gttgttggc gtgattgtg agtggcgat 
      aaatacgag tttgagatg aaagcgacg ggaaatgcc 
      ggatgggtg ggtgtgggg ctctcggat gatgataag 
      atgggagat gattcggtt attgagtgt gtgaaacgg 
      gggagcaac gttgctgcg ttcatgtcg tggaccagt 
      gggcctcct agttatggg gctgcgaga cttaataat 
      ccacaattg ggcataaaa ctggtgaac gcgagcata 
      atcaatgat gttttgtac tgcaaggta atgagagat 
      gagaggacg aaagttagg gggaaaatt tttgatctt 
      gctaatgaa aagtaccac attcttgta gcgtccggc 
      tcgcaagtc agtccaaat tctgtggca ttccacgac 
      cttgccttt ctggctagc ggtaacaag caatcactt 
      tcggacgtg tcagcactg gctgcggct tccaaactt 
      ttaatcaga cttcatggt tctttcatg ttggctgct 
      tggatcggt actgtttca gtgggaata cttttagca 
      cgttattac agaaatacg tgggttggg agcagcttg 
      tgtggaaaa gacctttgg tttgcttgg catcgaatg 
      tttatggtg ctgacctgg gccttgact gtgaccggg 
      tttgtcttg atttttgtc gaactgaga gcttggtca 
      gctgagaaa aacccgcac gctatttta gggactgtt 
      acaacgatt atttgcttt atccagccg attggggcg 
      tactttagg ccacatcct ggcactccg aagagacct 
      gtttttaac tggattcat tggctggga ggcaatgtg 
      gcacatatt atcggaatt gttacaatt ttcttcgct 
      gttaagtta acaaaagcc gaattgccg gactttgtg 
      gactggatt ttggtggca tatgtggcc ttccacgtc 
      ataattcat ttaatttta tcggtggtg gggtgtatt 
      tccgaaaaa tcgtttgaa agacgagtg acttccttc 
      cccatgaaa gatttaggt ggttctgga cgcagttcg 
      gcgtatgcg gacagaagc ggtgacgct ccgtactcc 
      ggtttccgc aagtttatc ttagccgtt tacattgta 
      atagtgcta gctatcgtc gttgcattg attgtcata 
      acagcccta gcacccatc gaagaatct tggaataat 
      ttgcgaaac tcgatgatg aatgccaaa taa
    mRNA sequence
    • >TC009118 RA
      cgtcacacc tgtcgacct tgcggccaa tgggaggct 
      cttgtgtac gctagcatt cactataca ttcaagaat 
      tcgagttgg gtaccacgt agtgcgtac ttctgaagc 
      atcctttct aacacaatt gtgagtgag gagtggaca 
      ataccagcg tggaaatga agtgtattg tgtgattca 
      gtatttttc tgaacgaga ttgtgaaaa ttaggtgat 
      cggtgtggt gttcagaga gaaatcttc gagtttgtg 
      cggctggat taaagtaaa caaggccat gctctggat 
      cctcctctt cctcctttc cctcggctc gatccaggc 
      ccttcccca aggcgcccc gaccaaggt atgccagac 
      catgaagcc gttccacgg aggaggcat ccctcctca 
      gcaggacat tgctccata ctcaatcta catgcgtcg 
      gttcaacgg tggcgttct cgtgacgct catatccga 
      cctgggaat cccattcca aggtttcat gctccaggc 
      caggacgcc caaccgaga gctcctggg agtgttcca 
      gccccgagg aaacggcag tcacaccat cgactgcgc 
      gcagtctgg ggacaccct cacccacaa cgacgccaa 
      caacaaggc gatggtgga ggtcgagtg gcacccgcc 
      agaggggta cgagggccc agtggtctt caacacgac 
      cattgcgca aaattacca aaccttctg ggtcggggt 
      ggagtcccg gactttgaa ggtgagtaa ggggttcac 
      agacgaccc catttcgaa catcccgag tcggagacc 
      atcgtcctc cacaccgcc cagtttttc ccccgagat 
      tagggaagt ggagactgt gtttgatcc cttctatga 
      aggctgcgg tgtcaccaa gctgtgttt tggcgcacc 
      ggccaattg catcaactc gaagaattg caaagccgt 
      tgttggcgt gattgtgag tggcgataa atacgagtt 
      tgagatgaa agcgacggg aaatgccgg atgggtggg 
      tgtggggct ctcggatga tgataagat gggagatga 
      ttcggttat tgagtgtgt gaaacgggg gagcaacgt 
      tgctgcgtt catgtcgtg gaccagtgg gcctcctag 
      ttatggggc tgcgagact taataatcc acaattggg 
      cataaaact ggtgaacgc gagcataat caatgatgt 
      tttgtactg caaggtaat gagagatga gaggacgaa 
      agttagggg gaaaatttt tgatcttgc taatgaaaa 
      gtaccacat tcttgtagc gtccggctc gcaagtcag 
      tccaaattc tgtggcatt ccacgacct tgcctttct 
      ggctagcgg taacaagca atcactttc ggacgtgtc 
      agcactggc tgcggcttc caaactttt aatcagact 
      tcatggttc tttcatgtt ggctgcttg gatcggtac 
      tgtttcagt gggaatact tttagcacg ttattacag 
      aaatacgtg ggttgggag cagcttgtg tggaaaaga 
      cctttggtt tgcttggca tcgaatgtt tatggtgct 
      gacctgggc cttgactgt gaccgggtt tgtcttgat 
      ttttgtcga actgagagc ttggtcagc tgagaaaaa 
      cccgcacgc tattttagg gactgttac aacgattat 
      ttgctttat ccagccgat tggggcgta ctttaggcc 
      acatcctgg cactccgaa gagacctgt ttttaactg 
      gattcattg gctgggagg caatgtggc acatattat 
      cggaattgt tacaatttt cttcgctgt taagttaac 
      aaaagccga attgccgga ctttgtgga ctggatttt 
      ggtggcata tgtggcctt ccacgtcat aattcattt 
      aattttatc ggtggtggg gtgtatttc cgaaaaatc 
      gtttgaaag acgagtgac ttccttccc catgaaaga 
      tttaggtgg ttctggacg cagttcggc gtatgcgga 
      cagaagcgg tgacgctcc gtactccgg tttccgcaa 
      gtttatctt agccgttta cattgtaat agtgctagc 
      tatcgtcgt tgcattgat tgtcataac agccctagc 
      acccatcga agaatcttg gaataattt gcgaaactc 
      gatgatgaa tgccaaata agacattac cagaaagta 
      ccaaataat ggcactatg cattatgtt aatgactga 
      ttatttttt attgtgaat gttttacag tatgcgaaa 
      agctgtgta cttgatagg tttagttga ataaaatgt 
      gaccactcc gtactaagc gctacaatt ttagaaaat 
      tactaactg cttttgcat tgacgagaa aattttgca 
      atcttatgt aaacaggtt accttcatt ggacaatca 
      cgcactttt atgtaacag gtattttgt aataattta 
      tgtttatga ttactgttt taattatta tttgttagg 
      taggtattt ttaattgtc aggggaaac ctgaattag 
      attaaaatt taaatatat ttgtaaata aagctatta 
      aacattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY