General information | |||
TC009118 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 4803036..4812174 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC009118 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01476 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Comment: body wall musculature without detectable defect |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
- >TC009118 RA
MLWILLFLL SLGSIQALP QGAPTKVCQ TMKPFHGGG IPPQQDIAP YSIYMRRFN GGVLVTLIS DLGIPFQGF MLQARTPNR ELLGVFQPR GNGSHTIDC AQSGDTLTH NDANNKAMV EVEWHPPEG YEGPVVFNT TIAQNYQTF WVGVESRTL KVSKGFTDD PISNIPSRR PSSSTPPSF SPEIREVET VFDPFYEGC GVTKLCFGA PANCINSKN CKAVVGVIV SGDKYEFEM KATGNAGWV GVGLSDDDK MGDDSVIEC VKRGSNVAA FMSWTSGPP SYGAARLNN PQLGIKLVN ASIINDVLY CKVMRDERT KVRGKIFDL ANEKYHILV ASGSQVSPN SVAFHDLAF LASGNKQSL SDVSALAAA SKLLIRLHG SFMLAAWIG TVSVGILLA RYYRNTWVG SSLCGKDLW FAWHRMFMV LTWALTVTG FVLIFVELR AWSAEKNPH AILGTVTTI ICFIQPIGA YFRPHPGTP KRPVFNWIH WLGGNVAHI IGIVTIFFA VKLTKAELP DFVDWILVA YVAFHVIIH LILSVVGCI SEKSFERRV TSFPMKDLG GSGRSSAYA DRSGDAPYS GFRKFILAV YIVIVLAIV VALIVITAL APIEESWNN LRNSMMNAK
- >TC009118 RA
atgctctgg atcctcctc ttcctcctt tccctcggc tcgatccag gcccttccc caaggcgcc ccgaccaag gtatgccag accatgaag ccgttccac ggaggaggc atccctcct cagcaggac attgctcca tactcaatc tacatgcgt cggttcaac ggtggcgtt ctcgtgacg ctcatatcc gacctggga atcccattc caaggtttc atgctccag gccaggacg cccaaccga gagctcctg ggagtgttc cagccccga ggaaacggc agtcacacc atcgactgc gcgcagtct ggggacacc ctcacccac aacgacgcc aacaacaag gcgatggtg gaggtcgag tggcacccg ccagagggg tacgagggc ccagtggtc ttcaacacg accattgcg caaaattac caaaccttc tgggtcggg gtggagtcc cggactttg aaggtgagt aaggggttc acagacgac cccatttcg aacatcccg agtcggaga ccatcgtcc tccacaccg cccagtttt tcccccgag attagggaa gtggagact gtgtttgat cccttctat gaaggctgc ggtgtcacc aagctgtgt tttggcgca ccggccaat tgcatcaac tcgaagaat tgcaaagcc gttgttggc gtgattgtg agtggcgat aaatacgag tttgagatg aaagcgacg ggaaatgcc ggatgggtg ggtgtgggg ctctcggat gatgataag atgggagat gattcggtt attgagtgt gtgaaacgg gggagcaac gttgctgcg ttcatgtcg tggaccagt gggcctcct agttatggg gctgcgaga cttaataat ccacaattg ggcataaaa ctggtgaac gcgagcata atcaatgat gttttgtac tgcaaggta atgagagat gagaggacg aaagttagg gggaaaatt tttgatctt gctaatgaa aagtaccac attcttgta gcgtccggc tcgcaagtc agtccaaat tctgtggca ttccacgac cttgccttt ctggctagc ggtaacaag caatcactt tcggacgtg tcagcactg gctgcggct tccaaactt ttaatcaga cttcatggt tctttcatg ttggctgct tggatcggt actgtttca gtgggaata cttttagca cgttattac agaaatacg tgggttggg agcagcttg tgtggaaaa gacctttgg tttgcttgg catcgaatg tttatggtg ctgacctgg gccttgact gtgaccggg tttgtcttg atttttgtc gaactgaga gcttggtca gctgagaaa aacccgcac gctatttta gggactgtt acaacgatt atttgcttt atccagccg attggggcg tactttagg ccacatcct ggcactccg aagagacct gtttttaac tggattcat tggctggga ggcaatgtg gcacatatt atcggaatt gttacaatt ttcttcgct gttaagtta acaaaagcc gaattgccg gactttgtg gactggatt ttggtggca tatgtggcc ttccacgtc ataattcat ttaatttta tcggtggtg gggtgtatt tccgaaaaa tcgtttgaa agacgagtg acttccttc cccatgaaa gatttaggt ggttctgga cgcagttcg gcgtatgcg gacagaagc ggtgacgct ccgtactcc ggtttccgc aagtttatc ttagccgtt tacattgta atagtgcta gctatcgtc gttgcattg attgtcata acagcccta gcacccatc gaagaatct tggaataat ttgcgaaac tcgatgatg aatgccaaa taa
- >TC009118 RA
cgtcacacc tgtcgacct tgcggccaa tgggaggct cttgtgtac gctagcatt cactataca ttcaagaat tcgagttgg gtaccacgt agtgcgtac ttctgaagc atcctttct aacacaatt gtgagtgag gagtggaca ataccagcg tggaaatga agtgtattg tgtgattca gtatttttc tgaacgaga ttgtgaaaa ttaggtgat cggtgtggt gttcagaga gaaatcttc gagtttgtg cggctggat taaagtaaa caaggccat gctctggat cctcctctt cctcctttc cctcggctc gatccaggc ccttcccca aggcgcccc gaccaaggt atgccagac catgaagcc gttccacgg aggaggcat ccctcctca gcaggacat tgctccata ctcaatcta catgcgtcg gttcaacgg tggcgttct cgtgacgct catatccga cctgggaat cccattcca aggtttcat gctccaggc caggacgcc caaccgaga gctcctggg agtgttcca gccccgagg aaacggcag tcacaccat cgactgcgc gcagtctgg ggacaccct cacccacaa cgacgccaa caacaaggc gatggtgga ggtcgagtg gcacccgcc agaggggta cgagggccc agtggtctt caacacgac cattgcgca aaattacca aaccttctg ggtcggggt ggagtcccg gactttgaa ggtgagtaa ggggttcac agacgaccc catttcgaa catcccgag tcggagacc atcgtcctc cacaccgcc cagtttttc ccccgagat tagggaagt ggagactgt gtttgatcc cttctatga aggctgcgg tgtcaccaa gctgtgttt tggcgcacc ggccaattg catcaactc gaagaattg caaagccgt tgttggcgt gattgtgag tggcgataa atacgagtt tgagatgaa agcgacggg aaatgccgg atgggtggg tgtggggct ctcggatga tgataagat gggagatga ttcggttat tgagtgtgt gaaacgggg gagcaacgt tgctgcgtt catgtcgtg gaccagtgg gcctcctag ttatggggc tgcgagact taataatcc acaattggg cataaaact ggtgaacgc gagcataat caatgatgt tttgtactg caaggtaat gagagatga gaggacgaa agttagggg gaaaatttt tgatcttgc taatgaaaa gtaccacat tcttgtagc gtccggctc gcaagtcag tccaaattc tgtggcatt ccacgacct tgcctttct ggctagcgg taacaagca atcactttc ggacgtgtc agcactggc tgcggcttc caaactttt aatcagact tcatggttc tttcatgtt ggctgcttg gatcggtac tgtttcagt gggaatact tttagcacg ttattacag aaatacgtg ggttgggag cagcttgtg tggaaaaga cctttggtt tgcttggca tcgaatgtt tatggtgct gacctgggc cttgactgt gaccgggtt tgtcttgat ttttgtcga actgagagc ttggtcagc tgagaaaaa cccgcacgc tattttagg gactgttac aacgattat ttgctttat ccagccgat tggggcgta ctttaggcc acatcctgg cactccgaa gagacctgt ttttaactg gattcattg gctgggagg caatgtggc acatattat cggaattgt tacaatttt cttcgctgt taagttaac aaaagccga attgccgga ctttgtgga ctggatttt ggtggcata tgtggcctt ccacgtcat aattcattt aattttatc ggtggtggg gtgtatttc cgaaaaatc gtttgaaag acgagtgac ttccttccc catgaaaga tttaggtgg ttctggacg cagttcggc gtatgcgga cagaagcgg tgacgctcc gtactccgg tttccgcaa gtttatctt agccgttta cattgtaat agtgctagc tatcgtcgt tgcattgat tgtcataac agccctagc acccatcga agaatcttg gaataattt gcgaaactc gatgatgaa tgccaaata agacattac cagaaagta ccaaataat ggcactatg cattatgtt aatgactga ttatttttt attgtgaat gttttacag tatgcgaaa agctgtgta cttgatagg tttagttga ataaaatgt gaccactcc gtactaagc gctacaatt ttagaaaat tactaactg cttttgcat tgacgagaa aattttgca atcttatgt aaacaggtt accttcatt ggacaatca cgcactttt atgtaacag gtattttgt aataattta tgtttatga ttactgttt taattatta tttgttagg taggtattt ttaattgtc aggggaaac ctgaattag attaaaatt taaatatat ttgtaaata aagctatta aacattt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
