Status
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Gene information
General information
TC009114 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4842982..4845946
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009114
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01475

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule, hours 48
    Pupal morphology
    remarks: later than schedule, hours 48
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    49

    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax segment partiallynumber decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs potentially partiallyfused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009114 RA
      MDVPPKSAT SKRESFAKS KSLSKNASV RLQRNRRLE 
      RQDSIGAIE ELIQCGICL EKLSDPKML PCQHTFCLP 
      CLHSHVMAK KILLKQASL ESQDVLAKK PIAIKCPVC 
      QNKFSLEKG IDSLNDLPK NLYIDSVLK LVECNIPPV 
      SSKPDRCVK CHIFSRGDE QCCQHCMQV FCNVCWSEH 
      LSELESNVS ILIKQVEES KIRLDHKLE SFENRCGQL 
      IDNVKEATE RKIELLRMD EERVLKEVE SIVREGKLN 
      HATISRKIG DLKNKLASN VMENSPGKV FSFMNLHKE 
      TSRLLSIVR HYGEARVIF DPETFKLDQ DTEGIYSDV 
      CDNENSTRS TNSLERFES ASHYYKNRS FVPKLVWNK 
      CPRPANVGV PPWDNRLLY ITATDTHTV LILNRDRRK 
      LEGRLISSE MLYPQGIAF SKTRQEIYV SDKWKHCIH 
      VFSKSRDYL RDLLSKGNG PGKVRSPDG IAVGPGDEL 
      VICDSGNDR IIIINPQTG EHISTIGTV GNKTTLNMP 
      TGVAMSGDK IIVADTGNH RIKIFYLDG RKLHEFGAL 
      GRGKGQFRS AEVVAVDSA GFILVGDGG NGRVQIFKP 
      DGSVAKIFG GSSQGSDGF GWVSGICVT SDLDIVVAD 
      SKTRSLRIY
    CDS sequence
    • >TC009114 RA
      atggatgtt cccccgaaa agtgctacc tctaaacga 
      gagtctttc gcaaaatct aaatcactg agtaaaaat 
      gcaagcgtt cgcctgcaa agaaatcgc cgactggaa 
      aggcaggac tccattggg gcaatcgaa gagctaatc 
      cagtgcgga atatgcctg gagaagctt tcggacccc 
      aaaatgctc ccctgccag cacactttc tgcttgccc 
      tgtctccac tcgcacgta atggccaaa aaaatcctc 
      ctcaagcaa gcgagtctc gaaagccaa gacgttctg 
      gctaaaaaa ccaattgct ataaaatgt cccgtctgc 
      cagaacaaa ttcagtttg gaaaagggc atagattcg 
      ttaaacgac ttgcccaaa aatctatac attgactct 
      gttctaaag ctggttgag tgtaacatt ccgccggtt 
      agttccaag cccgatcgt tgcgtcaaa tgccacatt 
      ttttcgcga ggggacgaa cagtgctgt cagcactgc 
      atgcaggtt ttttgcaat gtttgttgg agcgagcat 
      ttgtccgaa ctggaatcg aacgtttcg attttaatt 
      aaacaagtt gaagagtcg aaaattagg cttgatcac 
      aagttggag agttttgag aatcggtgc ggacaatta 
      attgataac gttaaagaa gcgactgag cgtaaaatc 
      gaattgttg aggatggac gaggagagg gttttgaag 
      gaagtcgag tcaattgtg cgcgaaggt aaattaaat 
      catgccaca atcagtcgc aaaattggg gatttgaaa 
      aataaactg gcgtctaac gttatggaa aattctccg 
      ggaaaggtt tttagtttc atgaatttg cataaggag 
      acgtcacgg cttctctcc atagttaga cactacggc 
      gaagctcgc gttattttt gacccagaa acattcaaa 
      ctggaccag gacacggaa gggatttat tcagacgtg 
      tgtgataac gaaaattcg acccgaagc actaactct 
      ttagaacga ttcgaatca gcaagtcac tattacaaa 
      aaccgatcg tttgtcccg aaattagtt tggaacaaa 
      tgtccacgg ccggcaaac gttggagtg cctccgtgg 
      gacaaccgc ttgctttat atcaccgca accgacacc 
      cacactgtg ctgatccta aaccgggac cgaagaaaa 
      ctcgaaggg agattaatt tcctccgag atgttgtac 
      ccccaaggc attgcattt tccaaaacc cgtcaagaa 
      atctacgtt tcggacaaa tggaagcac tgcattcac 
      gtcttctcc aaaagcagg gactaccta agagacttg 
      ctttcgaaa ggaaatggt cccgggaaa gtccgcagc 
      cctgacggc attgccgtg ggccccggc gacgaactt 
      gtgatttgt gacagcggg aacgacagg atcataata 
      attaacccc cagacaggg gagcatatc tccaccatc 
      ggcacggtg ggcaacaaa accacgttg aatatgcca 
      acgggggtg gcaatgagc ggcgataag ataatcgtg 
      gccgatacg gggaatcac agaattaag attttttac 
      ttggacgga aggaagttg cacgagttt ggggccttg 
      gggcgaggg aaggggcag tttaggtcg gctgaggtc 
      gtagctgtt gattctgca gggtttatt ttggtcggc 
      gatgggggt aatgggagg gtgcaaatt tttaagcct 
      gatggaagt gtcgcgaaa atttttggg gggtccagt 
      cagggtagt gacgggttt gggtgggtt tcggggatt 
      tgtgtgacg agtgatttg gatatcgtt gtggctgat 
      agcaagaca cgctcgttg aggatttat taa
    mRNA sequence
    • >TC009114 RA
      tatagcaat aaaattccg tcagcgatg tatcattcg 
      tgtttttgc atcgtaaca gctaattgc gcaaacacc 
      agtttgtaa agatattgc ttccaaaat ggatgttcc 
      cccgaaaag tgctacctc taaacgaga gtctttcgc 
      aaaatctaa atcactgag taaaaatgc aagcgttcg 
      cctgcaaag aaatcgccg actggaaag gcaggactc 
      cattggggc aatcgaaga gctaatcca gtgcggaat 
      atgcctgga gaagctttc ggaccccaa aatgctccc 
      ctgccagca cactttctg cttgccctg tctccactc 
      gcacgtaat ggccaaaaa aatcctcct caagcaagc 
      gagtctcga aagccaaga cgttctggc taaaaaacc 
      aattgctat aaaatgtcc cgtctgcca gaacaaatt 
      cagtttgga aaagggcat agattcgtt aaacgactt 
      gcccaaaaa tctatacat tgactctgt tctaaagct 
      ggttgagtg taacattcc gccggttag ttccaagcc 
      cgatcgttg cgtcaaatg ccacatttt ttcgcgagg 
      ggacgaaca gtgctgtca gcactgcat gcaggtttt 
      ttgcaatgt ttgttggag cgagcattt gtccgaact 
      ggaatcgaa cgtttcgat tttaattaa acaagttga 
      agagtcgaa aattaggct tgatcacaa gttggagag 
      ttttgagaa tcggtgcgg acaattaat tgataacgt 
      taaagaagc gactgagcg taaaatcga attgttgag 
      gatggacga ggagagggt tttgaagga agtcgagtc 
      aattgtgcg cgaaggtaa attaaatca tgccacaat 
      cagtcgcaa aattgggga tttgaaaaa taaactggc 
      gtctaacgt tatggaaaa ttctccggg aaaggtttt 
      tagtttcat gaatttgca taaggagac gtcacggct 
      tctctccat agttagaca ctacggcga agctcgcgt 
      tatttttga cccagaaac attcaaact ggaccagga 
      cacggaagg gatttattc agacgtgtg tgataacga 
      aaattcgac ccgaagcac taactcttt agaacgatt 
      cgaatcagc aagtcacta ttacaaaaa ccgatcgtt 
      tgtcccgaa attagtttg gaacaaatg tccacggcc 
      ggcaaacgt tggagtgcc tccgtggga caaccgctt 
      gctttatat caccgcaac cgacaccca cactgtgct 
      gatcctaaa ccgggaccg aagaaaact cgaagggag 
      attaatttc ctccgagat gttgtaccc ccaaggcat 
      tgcattttc caaaacccg tcaagaaat ctacgtttc 
      ggacaaatg gaagcactg cattcacgt cttctccaa 
      aagcaggga ctacctaag agacttgct ttcgaaagg 
      aaatggtcc cgggaaagt ccgcagccc tgacggcat 
      tgccgtggg ccccggcga cgaacttgt gatttgtga 
      cagcgggaa cgacaggat cataataat taaccccca 
      gacagggga gcatatctc caccatcgg cacggtggg 
      caacaaaac cacgttgaa tatgccaac gggggtggc 
      aatgagcgg cgataagat aatcgtggc cgatacggg 
      gaatcacag aattaagat tttttactt ggacggaag 
      gaagttgca cgagtttgg ggccttggg gcgagggaa 
      ggggcagtt taggtcggc tgaggtcgt agctgttga 
      ttctgcagg gtttatttt ggtcggcga tgggggtaa 
      tgggagggt gcaaatttt taagcctga tggaagtgt 
      cgcgaaaat ttttggggg gtccagtca gggtagtga 
      cgggtttgg gtgggtttc ggggatttg tgtgacgag 
      tgatttgga tatcgttgt ggctgatag caagacacg 
      ctcgttgag gatttatta aaagctggt tatttgttt 
      ctattttct ggtgtaggg tcggttttg ttttgtaat 
      ttacgtatt tgtacatta ataaaaaat attttttgt 
      a
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