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Gene information
General information
TC009113 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4851790..4853126
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009113
    iBeetle screen iB_01474

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: remnants inside-outed
    larva cuticle dorsal irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva segment size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009113 RA
      MTARNPTTL MAIPAEDTF DFLFKIVLI 
      GDCGTGKTC IVQRFKNGT FIERHGNTI 
      GVDFSMKTV MVDGKKVKL QIWDTAGQE 
      RFRTITQSY YRSANGVII VYDITKRSS 
      FLSVARWVE EVRRYSGSS VLLALVGNK 
      ADMESLREV EFEEAEAMC QYMPEVLFV 
      LEASAKDNS NIEEAFLCL ATELKRRHD 
      NGMLGNDDD DDTVKLGDS RAVTKCSSC 
      TQKIF
    CDS sequence
    • >TC009113 RA
      atgactgcg agaaatccg acaacgtta atggcaatt 
      ccagctgag gacacgttc gatttcctg tttaaaatc 
      gtgttaatt ggtgactgt ggcactggg aaaacttgc 
      atagttcaa agattcaaa aatgggaca tttatcgaa 
      agacacggc aatacgatc ggtgtggac ttttcaatg 
      aaaacagtg atggttgat gggaagaaa gtcaagctg 
      caaatatgg gacactgca ggtcaagaa agattccga 
      accatcact caaagttac tacagatct gcaaatggt 
      gtaatcata gtttatgac ataacgaaa cgctcctca 
      tttttatcg gtggcccgg tgggtggag gaggtcagg 
      aggtactca gggagctct gtcctactg gccttagtg 
      ggcaacaaa gctgacatg gaaagtttg agagaagtt 
      gagtttgag gaagccgaa gccatgtgc caatacatg 
      cctgaagtt ttgtttgtg ttagaagct agtgcgaaa 
      gataactca aatattgaa gaggcgttt ctttgtctt 
      gccaccgaa ttaaagaga cgacatgac aacggtatg 
      ttaggaaat gacgatgat gatgacact gtaaaatta 
      ggtgatagc agggctgta actaaatgc agtagttgc 
      acacaaaag atcttttaa
    mRNA sequence
    • >TC009113 RA
      agttttttg agtcatttt ttgtttaaa ttacgagtt 
      cgtggtcta aatgggggc atttgaacg tgaaataac 
      ccaccgggt cctgataat tttttttca tttaatttt 
      tttttgttg gcacccaag ttcaatgtt gaggtcagc 
      gccccctgc catatgtct aattataac ctacaagat 
      gggaggtaa ataaatatt tggtgattc tttggaata 
      gttgagaaa aggaaaatg actgcgaga aatccgaca 
      acgttaatg gcaattcca gctgaggac acgttcgat 
      ttcctgttt aaaatcgtg ttaattggt gactgtggc 
      actgggaaa acttgcata gttcaaaga ttcaaaaat 
      gggacattt atcgaaaga cacggcaat acgatcggt 
      gtggacttt tcaatgaaa acagtgatg gttgatggg 
      aagaaagtc aagctgcaa atatgggac actgcaggt 
      caagaaaga ttccgaacc atcactcaa agttactac 
      agatctgca aatggtgta atcatagtt tatgacata 
      acgaaacgc tcctcattt ttatcggtg gcccggtgg 
      gtggaggag gtcaggagg tactcaggg agctctgtc 
      ctactggcc ttagtgggc aacaaagct gacatggaa 
      agtttgaga gaagttgag tttgaggaa gccgaagcc 
      atgtgccaa tacatgcct gaagttttg tttgtgtta 
      gaagctagt gcgaaagat aactcaaat attgaagag 
      gcgtttctt tgtcttgcc accgaatta aagagacga 
      catgacaac ggtatgtta ggaaatgac gatgatgat 
      gacactgta aaattaggt gatagcagg gctgtaact 
      aaatgcagt agttgcaca caaaagatc ttttaatta 
      atataatga taacacaat tgtgcctta aatcatttt 
      gtgctttat gatattcga attttagtc gttattggt 
      tttagatat ctatcatct ctgcaagta atactcatt 
      tttacggtt gaatatttt tagtggatt tttttggcc 
      ggtttacaa gtgataatt acgtatttt tcaaagaaa 
      acggtaacg tagaatatt caaacgtat ttattatta 
      aagacttat taagggatt tgttaattt tattagaat 
      attcataca agaaatgtc caaataacg tatgtaacg 
      aaaataaaa tgtacttct taaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY