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Gene information
General information
TC009111 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4864010..4869626
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009111
    iBeetle screen iB_01473

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 4


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009111 RA
      MASVTEGDI ELLKNEIER LSRELDQAS SEKIQSAQY 
      GLVLLEEKT ELQRRCEDL EIAYENTKH ELKVTQEAL 
      AKFQSSHKV TTKTGIETE ESLLSESAA RETSLNSQI 
      IELENEIKQ LRHELDRVT TERDRMLQE NTDMGKDKE 
      DRSMECKKL RTELREIKF RENRLINDY AELEEENIA 
      LQKQVSALK SSQVEFESL KHEIRRLHE EMELLNLQV 
      EELTNLKKI AEKHMEEAL ESLQAEREA KYALKKELD 
      QHINRDTFY NINNLAYSI RGMSDEPTL GSDGEDDIP 
      GLKRLEATL GSGSASELA SPDDKQVDL FSEVHLNEL 
      RKLEKQLEQ LEKEKLTLT QNLRDNQAM VEKSQGELQ 
      VFIARLTQL AAHVDSLQH LSQNVDKNV SQPAQEAGK 
      ILNQYQQWF KMSCKEIEQ LKQDIRELE KLTNISDAT 
      LQLRTEITN LKNKLSDTE QRSMDLEAD LKLLREFAS 
      EAGTSLDET QNNLQTVTD ELAQLYHHV CTVNGETPT 
      RVVLDHEKE GMTPMSETA PPIGEMSKI ELLRSRLKS 
      DIPLHDLEV LNDAAVVAR NVCTIVDQI KHLRNAIEN 
      TIELSKTKR EKSNGLYES SKDENSEEI AELQEQVIR 
      LKSLLSTKR EQIATLRTV LKSNKNTAE VALTNLKAK 
      YENEKTIVS ETMMKLRNE LRMLKEDAA TFSSLRAMF 
      AARCEEYVT QVDELQQQL SAAEEEKKT LNQLLRLAV 
      QQKLGLTQR LEELEVDRE IRNARKPNN RNFQRPGRS 
      NRDMF
    CDS sequence
    • >TC009111 RA
      atggcttcg gttactgaa ggcgacatc gaactactc 
      aaaaacgaa attgaacga ctttcaaga gaactcgac 
      caagctagc agcgaaaaa atacagtct gctcagtac 
      ggccttgtg cttttggaa gaaaagacc gaattgcaa 
      agacgctgc gaggatctg gaaatcgcc tacgaaaac 
      actaagcat gaactaaaa gtcacacaa gaagccttg 
      gcaaagttc caatctagt cataaagta acaactaaa 
      actggaata gaaacggaa gaatccctg ctatctgaa 
      tccgccgcg cgcgaaaca tcgctcaat tcccaaata 
      atcgaattg gaaaacgaa ataaagcag ctgcgtcat 
      gagttggac cgcgtcacc accgagcga gaccgcatg 
      ctgcaggag aacaccgat atgggcaaa gacaaggaa 
      gatcgaagc atggagtgc aagaaatta cgaacggaa 
      ctccgcgaa atcaaattt cgagaaaat cgtttgatc 
      aacgactac gccgagcta gaagaagag aacatagcc 
      ttacaaaaa caagtctca gctttaaaa agctcgcaa 
      gtggagttt gaaagtcta aaacacgag atccggcga 
      ctgcacgaa gaaatggaa ctgttgaac ctccaggtg 
      gaagagctg acaaacttg aagaaaatc gccgagaaa 
      cacatggag gaggccttg gagtcgctg caagccgaa 
      cgtgaggcc aaatacgcg ctgaagaag gaactggac 
      caacatatt aaccgagac acgttctac aatatcaat 
      aatctggcg tacagtatt cggggaatg agcgatgag 
      ccgactttg gggagtgac ggtgaggac gatattccc 
      ggcttgaag cggctggag gcgactttg gggagtgga 
      agtgcctca gaattggct tcgcctgac gataaacag 
      gtcgatttg ttctccgaa gtccacttg aacgaactg 
      agaaagttg gaaaagcag ttggaacaa ctggagaag 
      gaaaaacta actctgaca caaaatttg cgcgataac 
      caggcaatg gtggagaag agtcaaggc gaactacaa 
      gttttcatt gcaagactg acacaactg gcggctcat 
      gtcgattct ttgcagcat ttgtcgcag aatgttgac 
      aaaaatgta tcccaaccg gcacaagag gctggtaaa 
      attctaaat cagtaccag cagtggttc aaaatgtcc 
      tgtaaagag atcgaacag ctgaagcag gatattcgt 
      gaattggag aagttgacg aatatctca gacgctacc 
      ctacagctg cgcaccgaa atcacgaat ttgaaaaat 
      aagttgtcg gatacggag cagaggagt atggacttg 
      gaggccgat ttgaaattg ttgagagag tttgctagt 
      gaagctgga acttcattg gatgaaact cagaataat 
      ttacaaact gttactgat gagcttgct cagttgtac 
      catcatgtg tgtactgtt aacggggag acgcccacg 
      agagtggtt ttggatcac gagaaggaa ggaatgacg 
      cctatgtcg gaaactgcg ccaccaatt ggcgaaatg 
      tccaaaatc gaattgttg cgaagccgc ctcaagagc 
      gacattccc ttgcacgat cttgaagtc ttaaatgac 
      gcggctgtt gtcgctcga aatgtttgc accatcgtg 
      gaccaaatc aaacatctc cgaaacgcc attgaaaat 
      accatcgaa ttatcgaaa acaaagagg gaaaaatcg 
      aatggatta tatgaatca agcaaagat gaaaacagc 
      gaagaaatc gccgaatta caagagcaa gtcataaga 
      ttgaagagt ttgctgtcg accaaacgc gaacagatt 
      gccacgtta cgaaccgtc ctaaaatca aacaaaaat 
      actgccgaa gtggcactg acgaacctc aaagccaag 
      tacgaaaac gagaaaaca attgtatcc gagacgatg 
      atgaagctc cgaaacgag ttgcgaatg ttgaaggag 
      gacgcagcc acattttca agtttgcgt gcgatgttt 
      gccgcccgt tgcgaagag tacgtgacg caagtcgac 
      gagctccag cagcagttg agcgcagcc gaagaagag 
      aagaagact ttgaaccag ctgttgaga ttagccgtt 
      cagcaaaag ctgggtctg actcagcgg ctcgaagag 
      ctggaggtg gacagagaa atcagaaac gcacgtaag 
      ccaaacaac cgcaatttt cagcgccct ggacggtcg 
      aacagagac atgttctag
    mRNA sequence
    • >TC009111 RA
      cgaaagtta ctaaacttt gaggttgac agttgacat 
      gttttgaac aaaaataca tttgttgca atgatgcag 
      tgatactta acagtttaa tttctaaca ttaattatc 
      tcttaattt ttattattt gggacaatg gcttcggtt 
      actgaaggc gacatcgaa ctactcaaa aacgaaatt 
      gaacgactt tcaagagaa ctcgaccaa gctagcagc 
      gaaaaaata cagtctgct cagtacggc cttgtgctt 
      ttggaagaa aagaccgaa ttgcaaaga cgctgcgag 
      gatctggaa atcgcctac gaaaacact aagcatgaa 
      ctaaaagtc acacaagaa gccttggca aagttccaa 
      tctagtcat aaagtaaca actaaaact ggaatagaa 
      acggaagaa tccctgcta tctgaatcc gccgcgcgc 
      gaaacatcg ctcaattcc caaataatc gaattggaa 
      aacgaaata aagcagctg cgtcatgag ttggaccgc 
      gtcaccacc gagcgagac cgcatgctg caggagaac 
      accgatatg ggcaaagac aaggaagat cgaagcatg 
      gagtgcaag aaattacga acggaactc cgcgaaatc 
      aaatttcga gaaaatcgt ttgatcaac gactacgcc 
      gagctagaa gaagagaac atagcctta caaaaacaa 
      gtctcagct ttaaaaagc tcgcaagtg gagtttgaa 
      agtctaaaa cacgagatc cggcgactg cacgaagaa 
      atggaactg ttgaacctc caggtggaa gagctgaca 
      aacttgaag aaaatcgcc gagaaacac atggaggag 
      gccttggag tcgctgcaa gccgaacgt gaggccaaa 
      tacgcgctg aagaaggaa ctggaccaa catattaac 
      cgagacacg ttctacaat atcaataat ctggcgtac 
      agtattcgg ggaatgagc gatgagccg actttgggg 
      agtgacggt gaggacgat attcccggc ttgaagcgg 
      ctggaggcg actttgggg agtggaagt gcctcagaa 
      ttggcttcg cctgacgat aaacaggtc gatttgttc 
      tccgaagtc cacttgaac gaactgaga aagttggaa 
      aagcagttg gaacaactg gagaaggaa aaactaact 
      ctgacacaa aatttgcgc gataaccag gcaatggtg 
      gagaagagt caaggcgaa ctacaagtt ttcattgca 
      agactgaca caactggcg gctcatgtc gattctttg 
      cagcatttg tcgcagaat gttgacaaa aatgtatcc 
      caaccggca caagaggct ggtaaaatt ctaaatcag 
      taccagcag tggttcaaa atgtcctgt aaagagatc 
      gaacagctg aagcaggat attcgtgaa ttggagaag 
      ttgacgaat atctcagac gctacccta cagctgcgc 
      accgaaatc acgaatttg aaaaataag ttgtcggat 
      acggagcag aggagtatg gacttggag gccgatttg 
      aaattgttg agagagttt gctagtgaa gctggaact 
      tcattggat gaaactcag aataattta caaactgtt 
      actgatgag cttgctcag ttgtaccat catgtgtgt 
      actgttaac ggggagacg cccacgaga gtggttttg 
      gatcacgag aaggaagga atgacgcct atgtcggaa 
      actgcgcca ccaattggc gaaatgtcc aaaatcgaa 
      ttgttgcga agccgcctc aagagcgac attcccttg 
      cacgatctt gaagtctta aatgacgcg gctgttgtc 
      gctcgaaat gtttgcacc atcgtggac caaatcaaa 
      catctccga aacgccatt gaaaatacc atcgaatta 
      tcgaaaaca aagagggaa aaatcgaat ggattatat 
      gaatcaagc aaagatgaa aacagcgaa gaaatcgcc 
      gaattacaa gagcaagtc ataagattg aagagtttg 
      ctgtcgacc aaacgcgaa cagattgcc acgttacga 
      accgtccta aaatcaaac aaaaatact gccgaagtg 
      gcactgacg aacctcaaa gccaagtac gaaaacgag 
      aaaacaatt gtatccgag acgatgatg aagctccga 
      aacgagttg cgaatgttg aaggaggac gcagccaca 
      ttttcaagt ttgcgtgcg atgtttgcc gcccgttgc 
      gaagagtac gtgacgcaa gtcgacgag ctccagcag 
      cagttgagc gcagccgaa gaagagaag aagactttg 
      aaccagctg ttgagatta gccgttcag caaaagctg 
      ggtctgact cagcggctc gaagagctg gaggtggac 
      agagaaatc agaaacgca cgtaagcca aacaaccgc 
      aattttcag cgccctgga cggtcgaac agagacatg 
      ttctagcgc aggtcgaaa cctgtggtt tacgcgatt 
      gcatttatc accacattc tttaaactt tgaaatttc 
      taacactac actttaatc ggctataac gtaacgaat 
      tgtgacaat tcacaaaaa ttcaagtat tttattgtt 
      tatttaacg aaatgtgag gtaaactgt gcatttatg 
      tgtttggtt tggcgactt tttatgcat actcagact 
      aatttattc tacttgtat tggctagta ctaattttc 
      ggtttagta aagtggcca attagctgc caaaagttt 
      cgtaattca gatttagtt atagattac gttcaagta 
      gcatttcta aggtatatc tcttgatta atagtggaa 
      tattatgac ggtagagta gcctatact tggatttaa 
      ttatgaaat gatatattt attggtaga tgtgaccca 
      tatttcgtg tgatgtata gactttaac catgtttta 
      gttattaac atatttatt tagtctact tagtcctac 
      aagggattc atgcaatat acgttagtt agtcattct 
      ttggtggtg tgaactaaa ataatattc gataaaccg 
      ctagtcttc gagactggt cacggtggc gctacgctc 
      gagggcatt tcgtctcga gaaaaatta aaaactgcg 
      tacatttat tttctaact gtggtacta aaatctaat 
      tgttacggt ttataaaaa atagttaaa acttgttaa 
      gacgtaggc taagaataa tattgtgaa gtgacggcg 
      aattgccgt cattctaat agtaaatat gatatcaca 
      attcgtctt ttccgttgg ttctgagtc catatgata 
      atcatgaaa tgacgtttg ttgattatt tgtcctgtt 
      tccgagcct atggaccac ggaaatgcc cgtccctta 
      caataatat aaaaatcct taatgttga gaatgaaat 
      gtgcatgct ctaacactt atatttttg ttacttggt 
      acacgtacc aacagaaac ttagatctt ctgtttgtt 
      tcgttcgtc agacagcta acctgttat attcttttt 
      gatatttat tgttataaa ttattttaa cattgctta 
      tatgtggtt aatgttact taattgtac ctaatattt 
      ttaatataa ttattactt tatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY