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Gene information
General information
TC009111 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4864010..4869626
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009111
    iBeetle screen iB_01473

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection

    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 4


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009111 RA
      MASVTEGDI ELLKNEIER LSRELDQAS SEKIQSAQY 
GLVLLEEKT ELQRRCEDL EIAYENTKH ELKVTQEAL 
AKFQSSHKV TTKTGIETE ESLLSESAA RETSLNSQI 
IELENEIKQ LRHELDRVT TERDRMLQE NTDMGKDKE 
DRSMECKKL RTELREIKF RENRLINDY AELEEENIA 
LQKQVSALK SSQVEFESL KHEIRRLHE EMELLNLQV 
EELTNLKKI AEKHMEEAL ESLQAEREA KYALKKELD 
QHINRDTFY NINNLAYSI RGMSDEPTL GSDGEDDIP 
GLKRLEATL GSGSASELA SPDDKQVDL FSEVHLNEL 
RKLEKQLEQ LEKEKLTLT QNLRDNQAM VEKSQGELQ 
VFIARLTQL AAHVDSLQH LSQNVDKNV SQPAQEAGK 
ILNQYQQWF KMSCKEIEQ LKQDIRELE KLTNISDAT 
LQLRTEITN LKNKLSDTE QRSMDLEAD LKLLREFAS 
EAGTSLDET QNNLQTVTD ELAQLYHHV CTVNGETPT 
RVVLDHEKE GMTPMSETA PPIGEMSKI ELLRSRLKS 
DIPLHDLEV LNDAAVVAR NVCTIVDQI KHLRNAIEN 
TIELSKTKR EKSNGLYES SKDENSEEI AELQEQVIR 
LKSLLSTKR EQIATLRTV LKSNKNTAE VALTNLKAK 
YENEKTIVS ETMMKLRNE LRMLKEDAA TFSSLRAMF 
AARCEEYVT QVDELQQQL SAAEEEKKT LNQLLRLAV 
QQKLGLTQR LEELEVDRE IRNARKPNN RNFQRPGRS 
NRDMF
    CDS sequence
    • >TC009111 RA
      atggcttcg gttactgaa ggcgacatc gaactactc 
aaaaacgaa attgaacga ctttcaaga gaactcgac 
caagctagc agcgaaaaa atacagtct gctcagtac 
ggccttgtg cttttggaa gaaaagacc gaattgcaa 
agacgctgc gaggatctg gaaatcgcc tacgaaaac 
actaagcat gaactaaaa gtcacacaa gaagccttg 
gcaaagttc caatctagt cataaagta acaactaaa 
actggaata gaaacggaa gaatccctg ctatctgaa 
tccgccgcg cgcgaaaca tcgctcaat tcccaaata 
atcgaattg gaaaacgaa ataaagcag ctgcgtcat 
gagttggac cgcgtcacc accgagcga gaccgcatg 
ctgcaggag aacaccgat atgggcaaa gacaaggaa 
gatcgaagc atggagtgc aagaaatta cgaacggaa 
ctccgcgaa atcaaattt cgagaaaat cgtttgatc 
aacgactac gccgagcta gaagaagag aacatagcc 
ttacaaaaa caagtctca gctttaaaa agctcgcaa 
gtggagttt gaaagtcta aaacacgag atccggcga 
ctgcacgaa gaaatggaa ctgttgaac ctccaggtg 
gaagagctg acaaacttg aagaaaatc gccgagaaa 
cacatggag gaggccttg gagtcgctg caagccgaa 
cgtgaggcc aaatacgcg ctgaagaag gaactggac 
caacatatt aaccgagac acgttctac aatatcaat 
aatctggcg tacagtatt cggggaatg agcgatgag 
ccgactttg gggagtgac ggtgaggac gatattccc 
ggcttgaag cggctggag gcgactttg gggagtgga 
agtgcctca gaattggct tcgcctgac gataaacag 
gtcgatttg ttctccgaa gtccacttg aacgaactg 
agaaagttg gaaaagcag ttggaacaa ctggagaag 
gaaaaacta actctgaca caaaatttg cgcgataac 
caggcaatg gtggagaag agtcaaggc gaactacaa 
gttttcatt gcaagactg acacaactg gcggctcat 
gtcgattct ttgcagcat ttgtcgcag aatgttgac 
aaaaatgta tcccaaccg gcacaagag gctggtaaa 
attctaaat cagtaccag cagtggttc aaaatgtcc 
tgtaaagag atcgaacag ctgaagcag gatattcgt 
gaattggag aagttgacg aatatctca gacgctacc 
ctacagctg cgcaccgaa atcacgaat ttgaaaaat 
aagttgtcg gatacggag cagaggagt atggacttg 
gaggccgat ttgaaattg ttgagagag tttgctagt 
gaagctgga acttcattg gatgaaact cagaataat 
ttacaaact gttactgat gagcttgct cagttgtac 
catcatgtg tgtactgtt aacggggag acgcccacg 
agagtggtt ttggatcac gagaaggaa ggaatgacg 
cctatgtcg gaaactgcg ccaccaatt ggcgaaatg 
tccaaaatc gaattgttg cgaagccgc ctcaagagc 
gacattccc ttgcacgat cttgaagtc ttaaatgac 
gcggctgtt gtcgctcga aatgtttgc accatcgtg 
gaccaaatc aaacatctc cgaaacgcc attgaaaat 
accatcgaa ttatcgaaa acaaagagg gaaaaatcg 
aatggatta tatgaatca agcaaagat gaaaacagc 
gaagaaatc gccgaatta caagagcaa gtcataaga 
ttgaagagt ttgctgtcg accaaacgc gaacagatt 
gccacgtta cgaaccgtc ctaaaatca aacaaaaat 
actgccgaa gtggcactg acgaacctc aaagccaag 
tacgaaaac gagaaaaca attgtatcc gagacgatg 
atgaagctc cgaaacgag ttgcgaatg ttgaaggag 
gacgcagcc acattttca agtttgcgt gcgatgttt 
gccgcccgt tgcgaagag tacgtgacg caagtcgac 
gagctccag cagcagttg agcgcagcc gaagaagag 
aagaagact ttgaaccag ctgttgaga ttagccgtt 
cagcaaaag ctgggtctg actcagcgg ctcgaagag 
ctggaggtg gacagagaa atcagaaac gcacgtaag 
ccaaacaac cgcaatttt cagcgccct ggacggtcg 
aacagagac atgttctag
    mRNA sequence
    • >TC009111 RA
      cgaaagtta ctaaacttt gaggttgac agttgacat 
gttttgaac aaaaataca tttgttgca atgatgcag 
tgatactta acagtttaa tttctaaca ttaattatc 
tcttaattt ttattattt gggacaatg gcttcggtt 
actgaaggc gacatcgaa ctactcaaa aacgaaatt 
gaacgactt tcaagagaa ctcgaccaa gctagcagc 
gaaaaaata cagtctgct cagtacggc cttgtgctt 
ttggaagaa aagaccgaa ttgcaaaga cgctgcgag 
gatctggaa atcgcctac gaaaacact aagcatgaa 
ctaaaagtc acacaagaa gccttggca aagttccaa 
tctagtcat aaagtaaca actaaaact ggaatagaa 
acggaagaa tccctgcta tctgaatcc gccgcgcgc 
gaaacatcg ctcaattcc caaataatc gaattggaa 
aacgaaata aagcagctg cgtcatgag ttggaccgc 
gtcaccacc gagcgagac cgcatgctg caggagaac 
accgatatg ggcaaagac aaggaagat cgaagcatg 
gagtgcaag aaattacga acggaactc cgcgaaatc 
aaatttcga gaaaatcgt ttgatcaac gactacgcc 
gagctagaa gaagagaac atagcctta caaaaacaa 
gtctcagct ttaaaaagc tcgcaagtg gagtttgaa 
agtctaaaa cacgagatc cggcgactg cacgaagaa 
atggaactg ttgaacctc caggtggaa gagctgaca 
aacttgaag aaaatcgcc gagaaacac atggaggag 
gccttggag tcgctgcaa gccgaacgt gaggccaaa 
tacgcgctg aagaaggaa ctggaccaa catattaac 
cgagacacg ttctacaat atcaataat ctggcgtac 
agtattcgg ggaatgagc gatgagccg actttgggg 
agtgacggt gaggacgat attcccggc ttgaagcgg 
ctggaggcg actttgggg agtggaagt gcctcagaa 
ttggcttcg cctgacgat aaacaggtc gatttgttc 
tccgaagtc cacttgaac gaactgaga aagttggaa 
aagcagttg gaacaactg gagaaggaa aaactaact 
ctgacacaa aatttgcgc gataaccag gcaatggtg 
gagaagagt caaggcgaa ctacaagtt ttcattgca 
agactgaca caactggcg gctcatgtc gattctttg 
cagcatttg tcgcagaat gttgacaaa aatgtatcc 
caaccggca caagaggct ggtaaaatt ctaaatcag 
taccagcag tggttcaaa atgtcctgt aaagagatc 
gaacagctg aagcaggat attcgtgaa ttggagaag 
ttgacgaat atctcagac gctacccta cagctgcgc 
accgaaatc acgaatttg aaaaataag ttgtcggat 
acggagcag aggagtatg gacttggag gccgatttg 
aaattgttg agagagttt gctagtgaa gctggaact 
tcattggat gaaactcag aataattta caaactgtt 
actgatgag cttgctcag ttgtaccat catgtgtgt 
actgttaac ggggagacg cccacgaga gtggttttg 
gatcacgag aaggaagga atgacgcct atgtcggaa 
actgcgcca ccaattggc gaaatgtcc aaaatcgaa 
ttgttgcga agccgcctc aagagcgac attcccttg 
cacgatctt gaagtctta aatgacgcg gctgttgtc 
gctcgaaat gtttgcacc atcgtggac caaatcaaa 
catctccga aacgccatt gaaaatacc atcgaatta 
tcgaaaaca aagagggaa aaatcgaat ggattatat 
gaatcaagc aaagatgaa aacagcgaa gaaatcgcc 
gaattacaa gagcaagtc ataagattg aagagtttg 
ctgtcgacc aaacgcgaa cagattgcc acgttacga 
accgtccta aaatcaaac aaaaatact gccgaagtg 
gcactgacg aacctcaaa gccaagtac gaaaacgag 
aaaacaatt gtatccgag acgatgatg aagctccga 
aacgagttg cgaatgttg aaggaggac gcagccaca 
ttttcaagt ttgcgtgcg atgtttgcc gcccgttgc 
gaagagtac gtgacgcaa gtcgacgag ctccagcag 
cagttgagc gcagccgaa gaagagaag aagactttg 
aaccagctg ttgagatta gccgttcag caaaagctg 
ggtctgact cagcggctc gaagagctg gaggtggac 
agagaaatc agaaacgca cgtaagcca aacaaccgc 
aattttcag cgccctgga cggtcgaac agagacatg 
ttctagcgc aggtcgaaa cctgtggtt tacgcgatt 
gcatttatc accacattc tttaaactt tgaaatttc 
taacactac actttaatc ggctataac gtaacgaat 
tgtgacaat tcacaaaaa ttcaagtat tttattgtt 
tatttaacg aaatgtgag gtaaactgt gcatttatg 
tgtttggtt tggcgactt tttatgcat actcagact 
aatttattc tacttgtat tggctagta ctaattttc 
ggtttagta aagtggcca attagctgc caaaagttt 
cgtaattca gatttagtt atagattac gttcaagta 
gcatttcta aggtatatc tcttgatta atagtggaa 
tattatgac ggtagagta gcctatact tggatttaa 
ttatgaaat gatatattt attggtaga tgtgaccca 
tatttcgtg tgatgtata gactttaac catgtttta 
gttattaac atatttatt tagtctact tagtcctac 
aagggattc atgcaatat acgttagtt agtcattct 
ttggtggtg tgaactaaa ataatattc gataaaccg 
ctagtcttc gagactggt cacggtggc gctacgctc 
gagggcatt tcgtctcga gaaaaatta aaaactgcg 
tacatttat tttctaact gtggtacta aaatctaat 
tgttacggt ttataaaaa atagttaaa acttgttaa 
gacgtaggc taagaataa tattgtgaa gtgacggcg 
aattgccgt cattctaat agtaaatat gatatcaca 
attcgtctt ttccgttgg ttctgagtc catatgata 
atcatgaaa tgacgtttg ttgattatt tgtcctgtt 
tccgagcct atggaccac ggaaatgcc cgtccctta 
caataatat aaaaatcct taatgttga gaatgaaat 
gtgcatgct ctaacactt atatttttg ttacttggt 
acacgtacc aacagaaac ttagatctt ctgtttgtt 
tcgttcgtc agacagcta acctgttat attcttttt 
gatatttat tgttataaa ttattttaa cattgctta 
tatgtggtt aatgttact taattgtac ctaatattt 
ttaatataa ttattactt tatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY