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Gene information
General information
TC009109 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4896379..4898376
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009109
    iBeetle screen iB_01472

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009109 RA
      MPEEIKQEV VLKTQKYLG KYVKKPPLS EKLLKKPPF 
      RFLHDIIKT VIKETGFLK GLFTEEELN SENVKEKEA 
      KVAFLTKLI DAVKAITNT DLAVRPSKI VAGLEPTET 
      NILLQTIGK AIEKKVDSS EYVKQLQSG EIINDKTRK 
      SKRPSDKNE KIKKTTSKT VINNEEKVR STPKVKEKH 
      SNETPKSTD REKSKPKRT SKESRSKPA SDDGKAKSK 
      EKPKKTKDT TKETSQKPS KESTEIPTT TAEIISPVD 
      NIDEKQNLT ETPESSVPE TVNTNLSVP DEESIKTII 
      PQITETPEA TEPRKRSAS ASRPKSARP KSGDLKEKT 
      TQDEVKETK TIQSRPKSS LRPPSVRPS SARPGAPRL 
      RPDSALPIK EVVPMGKIN VIVESFDKD GDEEETVII 
      QSNSEAVEE QVESTVEVS GGNQGHLVE QILEQINES 
      DVKVNAAKN DDDWQKDVF QGKDATTKE TNNLKSLIQ 
      TLTKTANPL GKLLNYLHE DIDAMQAEL EMWTNTKRQ 
      LFAEIAKQK KISLEMNKP LISQLEHLN QEITKMEEE 
      IASTRSNIL RNDRKIKEL LAK
    CDS sequence
    • >TC009109 RA
      atgcctgaa gagataaaa caagaagtg gtgctaaaa 
      acccaaaaa tatttagga aaatatgtg aaaaagccc 
      ccactcagt gaaaagtta ctcaaaaaa ccgcctttt 
      aggttttta cacgatatt attaagaca gttataaaa 
      gaaactggg tttttgaaa gggctcttc actgaagaa 
      gaattaaat tccgaaaat gtgaaagaa aaggaagcc 
      aaagtggct tttctcact aaattaatc gatgccgtt 
      aaagcaata acaaacaca gatttggct gttcgtccc 
      agtaaaatc gtcgcgggg cttgaacct accgaaact 
      aacattttg cttcaaact ataggaaaa gcaatagag 
      aaaaaagtt gatagttca gagtacgta aaacagtta 
      caatcaggt gaaataata aatgataag acgcgaaaa 
      tcaaaaaga ccctcagat aagaatgaa aaaattaag 
      aagacgact tcgaaaacg gtaataaat aacgaggag 
      aaagttcga agtactcca aaagtcaaa gaaaaacat 
      agcaatgaa accccaaaa tcaacggac agagaaaaa 
      tctaaacca aaacggaca tcaaaagaa agcagaagt 
      aaaccagca tcagacgat gggaaagca aagagtaaa 
      gagaagccg aaaaagact aaagacacg acaaaagaa 
      actagtcag aaaccaagt aaagaaagt accgaaatc 
      ccaacaacc actgctgaa attatctcc cctgttgat 
      aacattgac gaaaaacaa aatttaacc gaaacacct 
      gaaagttca gttcctgaa actgtcaat actaactta 
      tcggtacct gatgaagaa tctataaaa actatcatt 
      cctcaaatc accgaaaca cccgaagcc actgagcca 
      cgaaaacgt agcgcttcg gcctcgcgt cccaaatca 
      gctcgtcct aaaagcggc gatcttaaa gaaaaaaca 
      acccaggat gaagttaaa gaaactaag acgattcaa 
      agtcggccc aaaagctcg ttaaggcct ccaagtgtt 
      agaccttca agtgctcgg cctggggca cctcgtctg 
      cgacctgat tctgcactt cccatcaag gaagttgtt 
      cccatgggc aaaattaat gtaattgtt gaaagtttt 
      gacaaggat ggagacgaa gaggagacg gtgattatt 
      caaagcaat tcggaagct gtggaagaa caagtcgaa 
      agtaccgtg gaagtttcg gggggtaat caaggacat 
      ctcgttgag caaattttg gaacagatt aatgaaagt 
      gatgttaaa gttaatgca gctaaaaat gatgacgat 
      tggcagaaa gatgttttt caaggaaaa gatgctaca 
      actaaggaa acgaacaat ttaaaatcg ttgattcag 
      acgctgaca aagacagca aatccgtta ggaaaacta 
      ttaaattat cttcatgaa gatatcgat gcaatgcaa 
      gctgaactc gaaatgtgg acaaatacg aagcgtcag 
      ttgtttgct gaaattgcg aaacagaaa aaaataagt 
      ctggaaatg aataaacct ctgatatca caacttgag 
      cacttaaat caagagatc acgaaaatg gaagaagaa 
      attgcaagt accagaagc aatatcttg cgaaatgac 
      cgcaaaata aaggaactt ttagcaaaa taa
    mRNA sequence
    • >TC009109 RA
      tgtcaacag tgccacatg atgactttt gaggtggaa 
      tagtttagc gtaaaatgc ctgaagaga taaaacaag 
      aagtggtgc taaaaaccc aaaaatatt taggaaaat 
      atgtgaaaa agcccccac tcagtgaaa agttactca 
      aaaaaccgc cttttaggt ttttacacg atattatta 
      agacagtta taaaagaaa ctgggtttt tgaaagggc 
      tcttcactg aagaagaat taaattccg aaaatgtga 
      aagaaaagg aagccaaag tggcttttc tcactaaat 
      taatcgatg ccgttaaag caataacaa acacagatt 
      tggctgttc gtcccagta aaatcgtcg cggggcttg 
      aacctaccg aaactaaca ttttgcttc aaactatag 
      gaaaagcaa tagagaaaa aagttgata gttcagagt 
      acgtaaaac agttacaat caggtgaaa taataaatg 
      ataagacgc gaaaatcaa aaagaccct cagataaga 
      atgaaaaaa ttaagaaga cgacttcga aaacggtaa 
      taaataacg aggagaaag ttcgaagta ctccaaaag 
      tcaaagaaa aacatagca atgaaaccc caaaatcaa 
      cggacagag aaaaatcta aaccaaaac ggacatcaa 
      aagaaagca gaagtaaac cagcatcag acgatggga 
      aagcaaaga gtaaagaga agccgaaaa agactaaag 
      acacgacaa aagaaacta gtcagaaac caagtaaag 
      aaagtaccg aaatcccaa caaccactg ctgaaatta 
      tctcccctg ttgataaca ttgacgaaa aacaaaatt 
      taaccgaaa cacctgaaa gttcagttc ctgaaactg 
      tcaatacta acttatcgg tacctgatg aagaatcta 
      taaaaacta tcattcctc aaatcaccg aaacacccg 
      aagccactg agccacgaa aacgtagcg cttcggcct 
      cgcgtccca aatcagctc gtcctaaaa gcggcgatc 
      ttaaagaaa aaacaaccc aggatgaag ttaaagaaa 
      ctaagacga ttcaaagtc ggcccaaaa gctcgttaa 
      ggcctccaa gtgttagac cttcaagtg ctcggcctg 
      gggcacctc gtctgcgac ctgattctg cacttccca 
      tcaaggaag ttgttccca tgggcaaaa ttaatgtaa 
      ttgttgaaa gttttgaca aggatggag acgaagagg 
      agacggtga ttattcaaa gcaattcgg aagctgtgg 
      aagaacaag tcgaaagta ccgtggaag tttcggggg 
      gtaatcaag gacatctcg ttgagcaaa ttttggaac 
      agattaatg aaagtgatg ttaaagtta atgcagcta 
      aaaatgatg acgattggc agaaagatg tttttcaag 
      gaaaagatg ctacaacta aggaaacga acaatttaa 
      aatcgttga ttcagacgc tgacaaaga cagcaaatc 
      cgttaggaa aactattaa attatcttc atgaagata 
      tcgatgcaa tgcaagctg aactcgaaa tgtggacaa 
      atacgaagc gtcagttgt ttgctgaaa ttgcgaaac 
      agaaaaaaa taagtctgg aaatgaata aacctctga 
      tatcacaac ttgagcact taaatcaag agatcacga 
      aaatggaag aagaaattg caagtacca gaagcaata 
      tcttgcgaa atgaccgca aaataaagg aacttttag 
      caaaataaa ataaggttt aattacgtt atgtgcaat 
      tttgttgac tttatcttt gacacagtt aggtcacgt 
      ttactttct tcgcaaaat aagtatgga ca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY