Status
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Gene information
General information
TC009086 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7139937..7155309
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009086
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01468

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009086 RA
      MPVILLDRL PVPNLRMYS AGSVLALSA CVYYATQVI 
      KDPDWGAVA EDIKDSNRS NASVPLGRS MGQFVSDVF 
      SVMIREPIC VWTMINMAF CWLILVGTA IQKLVFGEL 
      RVSEQQHLK DKFWNFVFY KFIFVFGII NVQYIDEVL 
      LWCSWFSLL GFLHLLSQL SKDRFEYLS FSPTTPLWS 
      HVKLLFLLT AIFTLSGFM LLVSVFVGF FGGFNTFAF 
      MSAEVVLLS IRTLHVIIR YALHLYDMR QEGASSVSA 
      NEENRVWEK RGPIAYYTE LSFELTALI IDFVHHLHM 
      LVWSNIFLS MASLVICMQ LRYLFHEIQ RRYKKHRNY 
      LWVRNHLEQ NYPMASSEE LADNSDNCA ICWEKMESA 
      RKLPCTHLF HNTCLLSWL EQDTSCPTC RLALNIQTP 
      STSRIDPPD LQVDAPQPQ RRQLNHFFH FDGSRYVSW 
      LPSFSVEVS HAQLMRQDN APASNSQLD AMARQVQQL 
      FPHIPISTI INDLRVTRS VEITIENVL DGRLVAPTM 
      YREPEVVPA TNRAMSVWQ ASCDTDSQR NWDEFSVET 
      VETEDTSSL GSRFSKSSS EREKILHRR KENLIQSAR 
      KRYIEKQKA AAAAKVELQ S
    CDS sequence
    • >TC009086 RA
      atgccagtg attttactc gaccggttg cctgtgccc 
      aatctgcgg atgtactcc gccggcagt gtcttggcc 
      ctttcggcg tgcgtctac tacgccacg caggtgatc 
      aaggacccc gactggggg gccgtcgcc gaagacatc 
      aaagactcc aacagaagt aacgcctcg gtgccgctt 
      ggccggtcc atggggcag tttgtctcc gacgtgttc 
      agtgttatg ataagggag cccatctgc gtgtggacc 
      atgatcaac atggctttc tgttggctc atcctcgtg 
      ggtaccgcg atccagaag ctggtgttt ggcgaatta 
      cgcgtctct gaacagcaa catctcaaa gataagttt 
      tggaatttc gtattttat aaattcatc ttcgtattc 
      ggaatcatc aacgttcaa tacatcgat gaggtgttg 
      ctgtggtgc tcgtggttc tcccttttg ggatttctg 
      catctcctc tcccagctc agcaaagac agattcgaa 
      tatctgtct ttttcgccg actacgccg ctgtggagc 
      cacgtgaag cttttgttc ttgctaact gcgattttc 
      acactatcg ggcttcatg ctgctggtg tcggtcttt 
      gtgggattc ttcggcgga tttaatacg tttgctttc 
      atgtctgct gaggtggtg ttattgagt attcgtact 
      ctgcatgtg ataatccgg tacgccttg catctgtac 
      gacatgcga caagaaggt gcgtcttca gtcagtgca 
      aatgaagag aatcgagtt tgggaaaaa cgaggtccc 
      atcgcctat tacacggaa ttgagtttc gaactgaca 
      gctttaata atcgatttc gttcatcat cttcatatg 
      ctagtttgg agtaacatt ttcctctca atggctagt 
      ctagtgatt tgtatgcag ttgagatat ttgttccac 
      gagattcag cggcgctac aaaaaacac aggaattat 
      ctgtgggtc aggaatcat ctcgaacag aattacccg 
      atggcgtcg tccgaagag ttggccgac aattccgac 
      aactgcgcc atctgttgg gaaaaaatg gagtcggcg 
      aggaagtta ccgtgcact catttgttt cacaacact 
      tgtctgttg tcgtggctg gagcaagac acttcctgt 
      cccacttgt cgtctagcc ctcaacatt caaacgccc 
      tcaacgtcg cgaatcgat cctcccgat ttgcaagtg 
      gacgctcct cagccgcaa cgtcgccag ctcaatcat 
      ttctttcat ttcgacggt tcaagatac gtttcttgg 
      ttgccgagt ttttccgtc gaagtgagt catgctcaa 
      ctcatgcga caagacaac gcgccggcg tccaattcg 
      caattggat gcgatggca cgtcaagtg cagcaattg 
      tttccacac attccgatt tcgacaatt attaacgat 
      ctcagggta acgaggtcg gtggaaatc acgattgaa 
      aatgttttg gatggaaga ttggtagcg ccgactatg 
      tatagggag cccgaggtg gtgccggcg acgaatagg 
      gcgatgtcg gtatggcag gctagctgc gataccgac 
      tcgcagagg aattgggat gagttcagt gtggagacg 
      gttgaaact gaagatacg agcagtttg gggagcaga 
      ttttcgaag tcttcgtcg gagcgcgaa aagattctc 
      cacagacgc aaagagaat ttgattcag agcgccaga 
      aaacgctac atcgagaaa caaaaagcc gccgctgct 
      gcaaaagtg gagctacaa agttga
    mRNA sequence
    • >TC009086 RA
      tacagggcc ctgttttat ccggatatt tgacaggtg 
      catcagtgg cggcccgac cagctggcg caccacatg 
      atcaataca tgtataaaa ttaaccatc acgtaccag 
      tgtgtaatc aaaagtaac gtggcccca agcgcacca 
      catctccag tgacaaatc gttcgcggc cgcgactcc 
      acactatgt catcgtcgc gccccaatt ccgctttga 
      caagccgca caagagtcg cgttgtaat ttgtgacat 
      atcgtgttt gtaaacagt gacaatatg ccagtgatt 
      ttactcgac cggttgcct gtgcccaat ctgcggatg 
      tactccgcc ggcagtgtc ttggccctt tcggcgtgc 
      gtctactac gccacgcag gtgatcaag gaccccgac 
      tggggggcc gtcgccgaa gacatcaaa gactccaac 
      agaagtaac gcctcggtg ccgcttggc cggtccatg 
      gggcagttt gtctccgac gtgttcagt gttatgata 
      agggagccc atctgcgtg tggaccatg atcaacatg 
      gctttctgt tggctcatc ctcgtgggt accgcgatc 
      cagaagctg gtgtttggc gaattacgc gtctctgaa 
      cagcaacat ctcaaagat aagttttgg aatttcgta 
      ttttataaa ttcatcttc gtattcgga atcatcaac 
      gttcaatac atcgatgag gtgttgctg tggtgctcg 
      tggttctcc cttttggga tttctgcat ctcctctcc 
      cagctcagc aaagacaga ttcgaatat ctgtctttt 
      tcgccgact acgccgctg tggagccac gtgaagctt 
      ttgttcttg ctaactgcg attttcaca ctatcgggc 
      ttcatgctg ctggtgtcg gtctttgtg ggattcttc 
      ggcggattt aatacgttt gctttcatg tctgctgag 
      gtggtgtta ttgagtatt cgtactctg catgtgata 
      atccggtac gccttgcat ctgtacgac atgcgacaa 
      gaaggtgcg tcttcagtc agtgcaaat gaagagaat 
      cgagtttgg gaaaaacga ggtcccatc gcctattac 
      acggaattg agtttcgaa ctgacagct ttaataatc 
      gatttcgtt catcatctt catatgcta gtttggagt 
      aacattttc ctctcaatg gctagtcta gtgatttgt 
      atgcagttg agatatttg ttccacgag attcagcgg 
      cgctacaaa aaacacagg aattatctg tgggtcagg 
      aatcatctc gaacagaat tacccgatg gcgtcgtcc 
      gaagagttg gccgacaat tccgacaac tgcgccatc 
      tgttgggaa aaaatggag tcggcgagg aagttaccg 
      tgcactcat ttgtttcac aacacttgt ctgttgtcg 
      tggctggag caagacact tcctgtccc acttgtcgt 
      ctagccctc aacattcaa acgccctca acgtcgcga 
      atcgatcct cccgatttg caagtggac gctcctcag 
      ccgcaacgt cgccagctc aatcatttc tttcatttc 
      gacggttca agatacgtt tcttggttg ccgagtttt 
      tccgtcgaa gtgagtcat gctcaactc atgcgacaa 
      gacaacgcg ccggcgtcc aattcgcaa ttggatgcg 
      atggcacgt caagtgcag caattgttt ccacacatt 
      ccgatttcg acaattatt aacgatctc agggtaacg 
      aggtcggtg gaaatcacg attgaaaat gttttggat 
      ggaagattg gtagcgccg actatgtat agggagccc 
      gaggtggtg ccggcgacg aatagggcg atgtcggta 
      tggcaggct agctgcgat accgactcg cagaggaat 
      tgggatgag ttcagtgtg gagacggtt gaaactgaa 
      gatacgagc agtttgggg agcagattt tcgaagtct 
      tcgtcggag cgcgaaaag attctccac agacgcaaa 
      gagaatttg attcagagc gccagaaaa cgctacatc 
      gagaaacaa aaagccgcc gctgctgca aaagtggag 
      ctacaaagt tgaagtatt tatgaagat attttttag 
      gggagaagt cgtctgaca aagttgtcc cagatgtaa 
      caaattgtt aaagtgtac attgaaaat atctgcagt 
      taaaacact gcgctatat ttagatatt aataataaa 
      tgtctagat aacattatg taaaaattt gaattatct 
      tgcagaatt gtatttgtt ttattttcg aacgcaagg 
      ctcaaggga tgtggatct aaactaaaa atgataaaa 
      cgggaacgt gttttaaat accgattta ttaaagaca 
      aatcatttc gtaatggca gtttaggaa tatctaagt 
      tgttatggc gtttgtaag tttgtatga aattcatgt 
      tataaatta ttaagtagg tttaagata tttattagt 
      ctgaaattg aattgacgt cgattagtc attttccga 
      gtgataaat ttgcaattt gttgtttta tttgagaat 
      gtttatgtt ctttatttt tagatgaag taaataact 
      gtcttttta aacgaacta atgttcctt aatcaaagt 
      ttaataaaa cttagtcac cacttagcg gtgttacat 
      cgttagagc tagtctcac caatcggat ctggtgaac 
      ctcgccctg gatgaaata tttttaaag gctttggtc 
      agattgcac ttgaatttt aattaagtt tgtagtgta 
      cattgtgat aaaagactg actcttggt atttccgtg 
      tgatacttt cacgttttt gtgttaggt tttagattc 
      gtgctttat tttgctatg ttttaatgt ttgttttgt 
      ttacttaca cgcgatttt attacagtg ttaaaacgt 
      ccggcctga atttttgtg tataattaa aatgctcag 
      acccgaggg aatcctcgt aggtcgaac atgtatttc 
      atattgtta tgcagtgca aagtgcatg ttctcttta 
      ttaaacata gaactgtct agttatatt tcgatatag 
      gaaatacgt aattgccat gcaaacata ttgtgtgcc 
      agatatcta agggccaaa gtatatcag ttacttatt 
      tcgtcgtac ttacgtgtc ctgtcaatc tcctaatct 
      ttgttattg tgcttagta ttgctgtaa taaattatt 
      aaaaaaacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY