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Gene information
General information
TC009086 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7139937..7155309
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009086
    iBeetle screen iB_01468

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009086 RA
      MPVILLDRL PVPNLRMYS AGSVLALSA CVYYATQVI 
KDPDWGAVA EDIKDSNRS NASVPLGRS MGQFVSDVF 
SVMIREPIC VWTMINMAF CWLILVGTA IQKLVFGEL 
RVSEQQHLK DKFWNFVFY KFIFVFGII NVQYIDEVL 
LWCSWFSLL GFLHLLSQL SKDRFEYLS FSPTTPLWS 
HVKLLFLLT AIFTLSGFM LLVSVFVGF FGGFNTFAF 
MSAEVVLLS IRTLHVIIR YALHLYDMR QEGASSVSA 
NEENRVWEK RGPIAYYTE LSFELTALI IDFVHHLHM 
LVWSNIFLS MASLVICMQ LRYLFHEIQ RRYKKHRNY 
LWVRNHLEQ NYPMASSEE LADNSDNCA ICWEKMESA 
RKLPCTHLF HNTCLLSWL EQDTSCPTC RLALNIQTP 
STSRIDPPD LQVDAPQPQ RRQLNHFFH FDGSRYVSW 
LPSFSVEVS HAQLMRQDN APASNSQLD AMARQVQQL 
FPHIPISTI INDLRVTRS VEITIENVL DGRLVAPTM 
YREPEVVPA TNRAMSVWQ ASCDTDSQR NWDEFSVET 
VETEDTSSL GSRFSKSSS EREKILHRR KENLIQSAR 
KRYIEKQKA AAAAKVELQ S
    CDS sequence
    • >TC009086 RA
      atgccagtg attttactc gaccggttg cctgtgccc 
aatctgcgg atgtactcc gccggcagt gtcttggcc 
ctttcggcg tgcgtctac tacgccacg caggtgatc 
aaggacccc gactggggg gccgtcgcc gaagacatc 
aaagactcc aacagaagt aacgcctcg gtgccgctt 
ggccggtcc atggggcag tttgtctcc gacgtgttc 
agtgttatg ataagggag cccatctgc gtgtggacc 
atgatcaac atggctttc tgttggctc atcctcgtg 
ggtaccgcg atccagaag ctggtgttt ggcgaatta 
cgcgtctct gaacagcaa catctcaaa gataagttt 
tggaatttc gtattttat aaattcatc ttcgtattc 
ggaatcatc aacgttcaa tacatcgat gaggtgttg 
ctgtggtgc tcgtggttc tcccttttg ggatttctg 
catctcctc tcccagctc agcaaagac agattcgaa 
tatctgtct ttttcgccg actacgccg ctgtggagc 
cacgtgaag cttttgttc ttgctaact gcgattttc 
acactatcg ggcttcatg ctgctggtg tcggtcttt 
gtgggattc ttcggcgga tttaatacg tttgctttc 
atgtctgct gaggtggtg ttattgagt attcgtact 
ctgcatgtg ataatccgg tacgccttg catctgtac 
gacatgcga caagaaggt gcgtcttca gtcagtgca 
aatgaagag aatcgagtt tgggaaaaa cgaggtccc 
atcgcctat tacacggaa ttgagtttc gaactgaca 
gctttaata atcgatttc gttcatcat cttcatatg 
ctagtttgg agtaacatt ttcctctca atggctagt 
ctagtgatt tgtatgcag ttgagatat ttgttccac 
gagattcag cggcgctac aaaaaacac aggaattat 
ctgtgggtc aggaatcat ctcgaacag aattacccg 
atggcgtcg tccgaagag ttggccgac aattccgac 
aactgcgcc atctgttgg gaaaaaatg gagtcggcg 
aggaagtta ccgtgcact catttgttt cacaacact 
tgtctgttg tcgtggctg gagcaagac acttcctgt 
cccacttgt cgtctagcc ctcaacatt caaacgccc 
tcaacgtcg cgaatcgat cctcccgat ttgcaagtg 
gacgctcct cagccgcaa cgtcgccag ctcaatcat 
ttctttcat ttcgacggt tcaagatac gtttcttgg 
ttgccgagt ttttccgtc gaagtgagt catgctcaa 
ctcatgcga caagacaac gcgccggcg tccaattcg 
caattggat gcgatggca cgtcaagtg cagcaattg 
tttccacac attccgatt tcgacaatt attaacgat 
ctcagggta acgaggtcg gtggaaatc acgattgaa 
aatgttttg gatggaaga ttggtagcg ccgactatg 
tatagggag cccgaggtg gtgccggcg acgaatagg 
gcgatgtcg gtatggcag gctagctgc gataccgac 
tcgcagagg aattgggat gagttcagt gtggagacg 
gttgaaact gaagatacg agcagtttg gggagcaga 
ttttcgaag tcttcgtcg gagcgcgaa aagattctc 
cacagacgc aaagagaat ttgattcag agcgccaga 
aaacgctac atcgagaaa caaaaagcc gccgctgct 
gcaaaagtg gagctacaa agttga
    mRNA sequence
    • >TC009086 RA
      tacagggcc ctgttttat ccggatatt tgacaggtg 
catcagtgg cggcccgac cagctggcg caccacatg 
atcaataca tgtataaaa ttaaccatc acgtaccag 
tgtgtaatc aaaagtaac gtggcccca agcgcacca 
catctccag tgacaaatc gttcgcggc cgcgactcc 
acactatgt catcgtcgc gccccaatt ccgctttga 
caagccgca caagagtcg cgttgtaat ttgtgacat 
atcgtgttt gtaaacagt gacaatatg ccagtgatt 
ttactcgac cggttgcct gtgcccaat ctgcggatg 
tactccgcc ggcagtgtc ttggccctt tcggcgtgc 
gtctactac gccacgcag gtgatcaag gaccccgac 
tggggggcc gtcgccgaa gacatcaaa gactccaac 
agaagtaac gcctcggtg ccgcttggc cggtccatg 
gggcagttt gtctccgac gtgttcagt gttatgata 
agggagccc atctgcgtg tggaccatg atcaacatg 
gctttctgt tggctcatc ctcgtgggt accgcgatc 
cagaagctg gtgtttggc gaattacgc gtctctgaa 
cagcaacat ctcaaagat aagttttgg aatttcgta 
ttttataaa ttcatcttc gtattcgga atcatcaac 
gttcaatac atcgatgag gtgttgctg tggtgctcg 
tggttctcc cttttggga tttctgcat ctcctctcc 
cagctcagc aaagacaga ttcgaatat ctgtctttt 
tcgccgact acgccgctg tggagccac gtgaagctt 
ttgttcttg ctaactgcg attttcaca ctatcgggc 
ttcatgctg ctggtgtcg gtctttgtg ggattcttc 
ggcggattt aatacgttt gctttcatg tctgctgag 
gtggtgtta ttgagtatt cgtactctg catgtgata 
atccggtac gccttgcat ctgtacgac atgcgacaa 
gaaggtgcg tcttcagtc agtgcaaat gaagagaat 
cgagtttgg gaaaaacga ggtcccatc gcctattac 
acggaattg agtttcgaa ctgacagct ttaataatc 
gatttcgtt catcatctt catatgcta gtttggagt 
aacattttc ctctcaatg gctagtcta gtgatttgt 
atgcagttg agatatttg ttccacgag attcagcgg 
cgctacaaa aaacacagg aattatctg tgggtcagg 
aatcatctc gaacagaat tacccgatg gcgtcgtcc 
gaagagttg gccgacaat tccgacaac tgcgccatc 
tgttgggaa aaaatggag tcggcgagg aagttaccg 
tgcactcat ttgtttcac aacacttgt ctgttgtcg 
tggctggag caagacact tcctgtccc acttgtcgt 
ctagccctc aacattcaa acgccctca acgtcgcga 
atcgatcct cccgatttg caagtggac gctcctcag 
ccgcaacgt cgccagctc aatcatttc tttcatttc 
gacggttca agatacgtt tcttggttg ccgagtttt 
tccgtcgaa gtgagtcat gctcaactc atgcgacaa 
gacaacgcg ccggcgtcc aattcgcaa ttggatgcg 
atggcacgt caagtgcag caattgttt ccacacatt 
ccgatttcg acaattatt aacgatctc agggtaacg 
aggtcggtg gaaatcacg attgaaaat gttttggat 
ggaagattg gtagcgccg actatgtat agggagccc 
gaggtggtg ccggcgacg aatagggcg atgtcggta 
tggcaggct agctgcgat accgactcg cagaggaat 
tgggatgag ttcagtgtg gagacggtt gaaactgaa 
gatacgagc agtttgggg agcagattt tcgaagtct 
tcgtcggag cgcgaaaag attctccac agacgcaaa 
gagaatttg attcagagc gccagaaaa cgctacatc 
gagaaacaa aaagccgcc gctgctgca aaagtggag 
ctacaaagt tgaagtatt tatgaagat attttttag 
gggagaagt cgtctgaca aagttgtcc cagatgtaa 
caaattgtt aaagtgtac attgaaaat atctgcagt 
taaaacact gcgctatat ttagatatt aataataaa 
tgtctagat aacattatg taaaaattt gaattatct 
tgcagaatt gtatttgtt ttattttcg aacgcaagg 
ctcaaggga tgtggatct aaactaaaa atgataaaa 
cgggaacgt gttttaaat accgattta ttaaagaca 
aatcatttc gtaatggca gtttaggaa tatctaagt 
tgttatggc gtttgtaag tttgtatga aattcatgt 
tataaatta ttaagtagg tttaagata tttattagt 
ctgaaattg aattgacgt cgattagtc attttccga 
gtgataaat ttgcaattt gttgtttta tttgagaat 
gtttatgtt ctttatttt tagatgaag taaataact 
gtcttttta aacgaacta atgttcctt aatcaaagt 
ttaataaaa cttagtcac cacttagcg gtgttacat 
cgttagagc tagtctcac caatcggat ctggtgaac 
ctcgccctg gatgaaata tttttaaag gctttggtc 
agattgcac ttgaatttt aattaagtt tgtagtgta 
cattgtgat aaaagactg actcttggt atttccgtg 
tgatacttt cacgttttt gtgttaggt tttagattc 
gtgctttat tttgctatg ttttaatgt ttgttttgt 
ttacttaca cgcgatttt attacagtg ttaaaacgt 
ccggcctga atttttgtg tataattaa aatgctcag 
acccgaggg aatcctcgt aggtcgaac atgtatttc 
atattgtta tgcagtgca aagtgcatg ttctcttta 
ttaaacata gaactgtct agttatatt tcgatatag 
gaaatacgt aattgccat gcaaacata ttgtgtgcc 
agatatcta agggccaaa gtatatcag ttacttatt 
tcgtcgtac ttacgtgtc ctgtcaatc tcctaatct 
ttgttattg tgcttagta ttgctgtaa taaattatt 
aaaaaaacg
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