| General information | |||
| TC009086 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 7139937..7155309 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC009086 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01468 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC009086 RA
MPVILLDRL PVPNLRMYS AGSVLALSA CVYYATQVI KDPDWGAVA EDIKDSNRS NASVPLGRS MGQFVSDVF SVMIREPIC VWTMINMAF CWLILVGTA IQKLVFGEL RVSEQQHLK DKFWNFVFY KFIFVFGII NVQYIDEVL LWCSWFSLL GFLHLLSQL SKDRFEYLS FSPTTPLWS HVKLLFLLT AIFTLSGFM LLVSVFVGF FGGFNTFAF MSAEVVLLS IRTLHVIIR YALHLYDMR QEGASSVSA NEENRVWEK RGPIAYYTE LSFELTALI IDFVHHLHM LVWSNIFLS MASLVICMQ LRYLFHEIQ RRYKKHRNY LWVRNHLEQ NYPMASSEE LADNSDNCA ICWEKMESA RKLPCTHLF HNTCLLSWL EQDTSCPTC RLALNIQTP STSRIDPPD LQVDAPQPQ RRQLNHFFH FDGSRYVSW LPSFSVEVS HAQLMRQDN APASNSQLD AMARQVQQL FPHIPISTI INDLRVTRS VEITIENVL DGRLVAPTM YREPEVVPA TNRAMSVWQ ASCDTDSQR NWDEFSVET VETEDTSSL GSRFSKSSS EREKILHRR KENLIQSAR KRYIEKQKA AAAAKVELQ S
- >TC009086 RA
atgccagtg attttactc gaccggttg cctgtgccc aatctgcgg atgtactcc gccggcagt gtcttggcc ctttcggcg tgcgtctac tacgccacg caggtgatc aaggacccc gactggggg gccgtcgcc gaagacatc aaagactcc aacagaagt aacgcctcg gtgccgctt ggccggtcc atggggcag tttgtctcc gacgtgttc agtgttatg ataagggag cccatctgc gtgtggacc atgatcaac atggctttc tgttggctc atcctcgtg ggtaccgcg atccagaag ctggtgttt ggcgaatta cgcgtctct gaacagcaa catctcaaa gataagttt tggaatttc gtattttat aaattcatc ttcgtattc ggaatcatc aacgttcaa tacatcgat gaggtgttg ctgtggtgc tcgtggttc tcccttttg ggatttctg catctcctc tcccagctc agcaaagac agattcgaa tatctgtct ttttcgccg actacgccg ctgtggagc cacgtgaag cttttgttc ttgctaact gcgattttc acactatcg ggcttcatg ctgctggtg tcggtcttt gtgggattc ttcggcgga tttaatacg tttgctttc atgtctgct gaggtggtg ttattgagt attcgtact ctgcatgtg ataatccgg tacgccttg catctgtac gacatgcga caagaaggt gcgtcttca gtcagtgca aatgaagag aatcgagtt tgggaaaaa cgaggtccc atcgcctat tacacggaa ttgagtttc gaactgaca gctttaata atcgatttc gttcatcat cttcatatg ctagtttgg agtaacatt ttcctctca atggctagt ctagtgatt tgtatgcag ttgagatat ttgttccac gagattcag cggcgctac aaaaaacac aggaattat ctgtgggtc aggaatcat ctcgaacag aattacccg atggcgtcg tccgaagag ttggccgac aattccgac aactgcgcc atctgttgg gaaaaaatg gagtcggcg aggaagtta ccgtgcact catttgttt cacaacact tgtctgttg tcgtggctg gagcaagac acttcctgt cccacttgt cgtctagcc ctcaacatt caaacgccc tcaacgtcg cgaatcgat cctcccgat ttgcaagtg gacgctcct cagccgcaa cgtcgccag ctcaatcat ttctttcat ttcgacggt tcaagatac gtttcttgg ttgccgagt ttttccgtc gaagtgagt catgctcaa ctcatgcga caagacaac gcgccggcg tccaattcg caattggat gcgatggca cgtcaagtg cagcaattg tttccacac attccgatt tcgacaatt attaacgat ctcagggta acgaggtcg gtggaaatc acgattgaa aatgttttg gatggaaga ttggtagcg ccgactatg tatagggag cccgaggtg gtgccggcg acgaatagg gcgatgtcg gtatggcag gctagctgc gataccgac tcgcagagg aattgggat gagttcagt gtggagacg gttgaaact gaagatacg agcagtttg gggagcaga ttttcgaag tcttcgtcg gagcgcgaa aagattctc cacagacgc aaagagaat ttgattcag agcgccaga aaacgctac atcgagaaa caaaaagcc gccgctgct gcaaaagtg gagctacaa agttga
- >TC009086 RA
tacagggcc ctgttttat ccggatatt tgacaggtg catcagtgg cggcccgac cagctggcg caccacatg atcaataca tgtataaaa ttaaccatc acgtaccag tgtgtaatc aaaagtaac gtggcccca agcgcacca catctccag tgacaaatc gttcgcggc cgcgactcc acactatgt catcgtcgc gccccaatt ccgctttga caagccgca caagagtcg cgttgtaat ttgtgacat atcgtgttt gtaaacagt gacaatatg ccagtgatt ttactcgac cggttgcct gtgcccaat ctgcggatg tactccgcc ggcagtgtc ttggccctt tcggcgtgc gtctactac gccacgcag gtgatcaag gaccccgac tggggggcc gtcgccgaa gacatcaaa gactccaac agaagtaac gcctcggtg ccgcttggc cggtccatg gggcagttt gtctccgac gtgttcagt gttatgata agggagccc atctgcgtg tggaccatg atcaacatg gctttctgt tggctcatc ctcgtgggt accgcgatc cagaagctg gtgtttggc gaattacgc gtctctgaa cagcaacat ctcaaagat aagttttgg aatttcgta ttttataaa ttcatcttc gtattcgga atcatcaac gttcaatac atcgatgag gtgttgctg tggtgctcg tggttctcc cttttggga tttctgcat ctcctctcc cagctcagc aaagacaga ttcgaatat ctgtctttt tcgccgact acgccgctg tggagccac gtgaagctt ttgttcttg ctaactgcg attttcaca ctatcgggc ttcatgctg ctggtgtcg gtctttgtg ggattcttc ggcggattt aatacgttt gctttcatg tctgctgag gtggtgtta ttgagtatt cgtactctg catgtgata atccggtac gccttgcat ctgtacgac atgcgacaa gaaggtgcg tcttcagtc agtgcaaat gaagagaat cgagtttgg gaaaaacga ggtcccatc gcctattac acggaattg agtttcgaa ctgacagct ttaataatc gatttcgtt catcatctt catatgcta gtttggagt aacattttc ctctcaatg gctagtcta gtgatttgt atgcagttg agatatttg ttccacgag attcagcgg cgctacaaa aaacacagg aattatctg tgggtcagg aatcatctc gaacagaat tacccgatg gcgtcgtcc gaagagttg gccgacaat tccgacaac tgcgccatc tgttgggaa aaaatggag tcggcgagg aagttaccg tgcactcat ttgtttcac aacacttgt ctgttgtcg tggctggag caagacact tcctgtccc acttgtcgt ctagccctc aacattcaa acgccctca acgtcgcga atcgatcct cccgatttg caagtggac gctcctcag ccgcaacgt cgccagctc aatcatttc tttcatttc gacggttca agatacgtt tcttggttg ccgagtttt tccgtcgaa gtgagtcat gctcaactc atgcgacaa gacaacgcg ccggcgtcc aattcgcaa ttggatgcg atggcacgt caagtgcag caattgttt ccacacatt ccgatttcg acaattatt aacgatctc agggtaacg aggtcggtg gaaatcacg attgaaaat gttttggat ggaagattg gtagcgccg actatgtat agggagccc gaggtggtg ccggcgacg aatagggcg atgtcggta tggcaggct agctgcgat accgactcg cagaggaat tgggatgag ttcagtgtg gagacggtt gaaactgaa gatacgagc agtttgggg agcagattt tcgaagtct tcgtcggag cgcgaaaag attctccac agacgcaaa gagaatttg attcagagc gccagaaaa cgctacatc gagaaacaa aaagccgcc gctgctgca aaagtggag ctacaaagt tgaagtatt tatgaagat attttttag gggagaagt cgtctgaca aagttgtcc cagatgtaa caaattgtt aaagtgtac attgaaaat atctgcagt taaaacact gcgctatat ttagatatt aataataaa tgtctagat aacattatg taaaaattt gaattatct tgcagaatt gtatttgtt ttattttcg aacgcaagg ctcaaggga tgtggatct aaactaaaa atgataaaa cgggaacgt gttttaaat accgattta ttaaagaca aatcatttc gtaatggca gtttaggaa tatctaagt tgttatggc gtttgtaag tttgtatga aattcatgt tataaatta ttaagtagg tttaagata tttattagt ctgaaattg aattgacgt cgattagtc attttccga gtgataaat ttgcaattt gttgtttta tttgagaat gtttatgtt ctttatttt tagatgaag taaataact gtcttttta aacgaacta atgttcctt aatcaaagt ttaataaaa cttagtcac cacttagcg gtgttacat cgttagagc tagtctcac caatcggat ctggtgaac ctcgccctg gatgaaata tttttaaag gctttggtc agattgcac ttgaatttt aattaagtt tgtagtgta cattgtgat aaaagactg actcttggt atttccgtg tgatacttt cacgttttt gtgttaggt tttagattc gtgctttat tttgctatg ttttaatgt ttgttttgt ttacttaca cgcgatttt attacagtg ttaaaacgt ccggcctga atttttgtg tataattaa aatgctcag acccgaggg aatcctcgt aggtcgaac atgtatttc atattgtta tgcagtgca aagtgcatg ttctcttta ttaaacata gaactgtct agttatatt tcgatatag gaaatacgt aattgccat gcaaacata ttgtgtgcc agatatcta agggccaaa gtatatcag ttacttatt tcgtcgtac ttacgtgtc ctgtcaatc tcctaatct ttgttattg tgcttagta ttgctgtaa taaattatt aaaaaaacg
