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Gene information
General information
TC033679
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009077
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 605986..610252
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033679
    iBeetle screen iB_01467
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033679 RA
      MGSLPRTSK SSLSAGTPD SLSDNESSA 
      FRTPRKPSY RSTMTPGGS RSNSRPASR 
      TGSRPGSKP PSRHGSNLS LDSTDDGTP 
      SRIPRRTPT SSRVPSTTS SLRRPQNGT 
      GSRPRTPTG LISPASPAG KSSGIPRAS 
      SIPTLTSVT TPRSTSKPR QVTIGNAPT 
      LRTAARTRT PSGSNTPVP PGQQTAASR 
      LLRKPSGAS DTPTGQAKK TTKEREPFR 
      L
    CDS sequence
    • >TC033679 RA
      atgggcagc cttccgcgc acttccaaa tcctcctta 
      tcagcgggg acccccgac tcgttatca gacaacgaa 
      agttctgct ttccgcacg cccagaaaa ccatcttac 
      aggtcaacg atgacccca ggagggtct aggtcgaac 
      tcacgaccg gcgtcgcga actggcagc aggcctggc 
      agcaaaccc ccatcgaga catggttct aacctctcc 
      ctagatagt acagatgat ggtaccccg tcgaggatt 
      ccacgaagg accccaacg tcaagtcgg gtaccatcg 
      acaacttct tctttgcgg aggccccag aatggcacc 
      gggtcccgg ccgaggaca cccacgggg ctcatttca 
      ccagctagt ccagccgga aaatcatca ggcattccc 
      cgagcatca agtatacca acactaacg tcagttacc 
      accccaagg agtacaagc aaaccacga caagtgaca 
      atcggtaac gctccgacg ctaaggacg gcggctagg 
      acacgaaca ccatccggg tctaacaca cccgttccg 
      cccgggcaa caaaccgcc gcttcccgc cttctacga 
      aaaccatcg ggagcgtcc gacacgcca acagggcaa 
      gcgaagaaa acaacaaag gaacgggag ccttttaga 
      ctttaa
    mRNA sequence
    • >TC033679 RA
      aatttgaga ggtcctaaa ccatagttt tatttccac 
      tgtaatata cgcacacgg gacgatctc ttcccgttt 
      tagcaatta gtgcatttc cttttattt ttatttttt 
      tagaatttt tgcttattt tgtttttgt ttttttttc 
      tcctttcac gtgtcccct accgcacta acttgtttt 
      ctgttactt acttccttt tcctcgtcc tgaaattcc 
      tcacacagc caaaggacg cacgaacat agagctgcg 
      tgaacaatt catcctagc cgacggcgt atcgcaaac 
      tatgaccgc gttcaagcc caaaacatc ggcctccag 
      tcccgcctc tggctcctc tgcatcatc actccgcac 
      tcctcaagg gccaatcac taaggtatt gcacaagag 
      caccctctt tgatccgga aaaagcatc gaaaatact 
      atccttgtc ctcgatccc agtcggttg tggagtatt 
      gttttcagt gcttttttc gagttgctc tatgtcttg 
      gtaatggta cgcttgtat acgtagtac agtagttgg 
      ccaccagag ggtaaaggt aagtcggac ctcaggaga 
      tgaattttg tgaacgcga caacaacaa gggccgaag 
      cataaaaca aaatcgcct cacgttcat tcagcactc 
      gatgcttct tttttctgt tcgcaccat ctaaacgcg 
      acgatcact attggatta tttcggaca cgctgtata 
      tttctgaat gttggcatg tgctattgt ttagctcac 
      gaacacttg gcggagttg atgcccata ttcgagcag 
      ctgagggcc aaaggagaa gtgggcaat gcgtaccct 
      ttgcatgtc ggttctact gctggaccc accttacac 
      tgggctcct ctcaatatc aacaacact tgtcactgg 
      gtaataata cacacagac actttgtgc atgtgcatg 
      gtgtgtacg tctcttctc tcgtttttt ttgcaaaat 
      catcattta tcgttaaat cgagtcgag tttcaatcg 
      taccgtttt tcttgtttt tataccagt cgctgttat 
      cgggccgga aaaagacca ccacaaact tttgtacct 
      atcgcaagt accgatggt aggtatacc gcaggtaag 
      acgaataaa tcgctcatt acttttggt ctttctttc 
      caaaatttc taaaaccgg cccaatttt tactaagat 
      cattttaac gttaccgga tgaggtaag acaggaaac 
      tgtaagtag cgttttgtt atgcaatca aggatcttc 
      aatttacat tttttaatc acacacttg ttaacgtag 
      cgtcttgtc actgttatc taacacgaa gatcctttt 
      tttgtgggt gcacgactc gtatcaatt gttggccct 
      taccaacaa ctggtactc ttccactca cccatcctt 
      actaaaaat tactgttta ggtgcgaga gcgttcagc 
      aaggtcggt tccaatggg cagccttcc gcgcacttc 
      caaatcctc cttatcagc ggggacccc cgactcgtt 
      atcagacaa cgaaagttc tgctttccg cacgcccag 
      aaaaccatc ttacaggtc aacgatgac cccaggagg 
      gtctaggtc gaactcacg accggcgtc gcgaactgg 
      cagcaggcc tggcagcaa acccccatc gagacatgg 
      ttctaacct ctccctaga tagtacaga tgatggtac 
      cccgtcgag gattccacg aaggacccc aacgtcaag 
      tcgggtacc atcgacaac ttcttcttt gcggaggcc 
      ccagaatgg caccgggtc ccggccgag gacacccac 
      ggggctcat ttcaccagc tagtccagc cggaaaatc 
      atcaggcat tccccgagc atcaagtat accaacact 
      aacgtcagt taccacccc aaggagtac aagcaaacc 
      acgacaagt gacaatcgg taacgctcc gacgctaag 
      gacggcggc taggacacg aacaccatc cgggtctaa 
      cacacccgt tccgcccgg gcaacaaac cgccgcttc 
      ccgccttct acgaaaacc atcgggagc gtccgacac 
      gccaacagg gcaagcgaa gaaaacaac aaaggaacg 
      ggagccttt tagacttta attaattat gagattaac 
      ttattccaa gattaacga atgactgac agtagggtt 
      attgtgttt ttgtttaaa ttgttcata tttttgtat 
      aaaagattt atttatgaa gcggccccg gaaacgcca 
      gttccggag ttgaattat tgtgtgagt cacaatacg 
      taactgcgt taagttgcc ataaattgc aaaattatt 
      ttcgtgagg tgatttttg tattatttt ttgggtatc 
      agtgggacc attttgtgg cgatttgct ttgtttagt 
      ggacgttta tttgattta tcctactaa cttacttat 
      tttatcctt aataattta gatactaaa gcgccgcta 
      atactcgta gttgtgtga ttaccagaa acgcctttc 
      ttttatatc gccagtatg aaagaacgg atcagtcaa 
      tattgtaac tattactat atttaattt gttatgtat 
      taaaacaat aaactaaat tttttaag