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Gene information
General information
TC033679
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009077 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7273333..7277599
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033679
    iBeetle screen iB_01467

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033679 RA
      MGSLPRTSK SSLSAGTPD SLSDNESSA 
FRTPRKPSY RSTMTPGGS RSNSRPASR 
TGSRPGSKP PSRHGSNLS LDSTDDGTP 
SRIPRRTPT SSRVPSTTS SLRRPQNGT 
GSRPRTPTG LISPASPAG KSSGIPRAS 
SIPTLTSVT TPRSTSKPR QVTIGNAPT 
LRTAARTRT PSGSNTPVP PGQQTAASR 
LLRKPSGAS DTPTGQAKK TTKEREPFR 
L
    CDS sequence
    • >TC033679 RA
      atgggcagc cttccgcgc acttccaaa tcctcctta 
tcagcgggg acccccgac tcgttatca gacaacgaa 
agttctgct ttccgcacg cccagaaaa ccatcttac 
aggtcaacg atgacccca ggagggtct aggtcgaac 
tcacgaccg gcgtcgcga actggcagc aggcctggc 
agcaaaccc ccatcgaga catggttct aacctctcc 
ctagatagt acagatgat ggtaccccg tcgaggatt 
ccacgaagg accccaacg tcaagtcgg gtaccatcg 
acaacttct tctttgcgg aggccccag aatggcacc 
gggtcccgg ccgaggaca cccacgggg ctcatttca 
ccagctagt ccagccgga aaatcatca ggcattccc 
cgagcatca agtatacca acactaacg tcagttacc 
accccaagg agtacaagc aaaccacga caagtgaca 
atcggtaac gctccgacg ctaaggacg gcggctagg 
acacgaaca ccatccggg tctaacaca cccgttccg 
cccgggcaa caaaccgcc gcttcccgc cttctacga 
aaaccatcg ggagcgtcc gacacgcca acagggcaa 
gcgaagaaa acaacaaag gaacgggag ccttttaga 
ctttaa
    mRNA sequence
    • >TC033679 RA
      aatttgaga ggtcctaaa ccatagttt tatttccac 
tgtaatata cgcacacgg gacgatctc ttcccgttt 
tagcaatta gtgcatttc cttttattt ttatttttt 
tagaatttt tgcttattt tgtttttgt ttttttttc 
tcctttcac gtgtcccct accgcacta acttgtttt 
ctgttactt acttccttt tcctcgtcc tgaaattcc 
tcacacagc caaaggacg cacgaacat agagctgcg 
tgaacaatt catcctagc cgacggcgt atcgcaaac 
tatgaccgc gttcaagcc caaaacatc ggcctccag 
tcccgcctc tggctcctc tgcatcatc actccgcac 
tcctcaagg gccaatcac taaggtatt gcacaagag 
caccctctt tgatccgga aaaagcatc gaaaatact 
atccttgtc ctcgatccc agtcggttg tggagtatt 
gttttcagt gcttttttc gagttgctc tatgtcttg 
gtaatggta cgcttgtat acgtagtac agtagttgg 
ccaccagag ggtaaaggt aagtcggac ctcaggaga 
tgaattttg tgaacgcga caacaacaa gggccgaag 
cataaaaca aaatcgcct cacgttcat tcagcactc 
gatgcttct tttttctgt tcgcaccat ctaaacgcg 
acgatcact attggatta tttcggaca cgctgtata 
tttctgaat gttggcatg tgctattgt ttagctcac 
gaacacttg gcggagttg atgcccata ttcgagcag 
ctgagggcc aaaggagaa gtgggcaat gcgtaccct 
ttgcatgtc ggttctact gctggaccc accttacac 
tgggctcct ctcaatatc aacaacact tgtcactgg 
gtaataata cacacagac actttgtgc atgtgcatg 
gtgtgtacg tctcttctc tcgtttttt ttgcaaaat 
catcattta tcgttaaat cgagtcgag tttcaatcg 
taccgtttt tcttgtttt tataccagt cgctgttat 
cgggccgga aaaagacca ccacaaact tttgtacct 
atcgcaagt accgatggt aggtatacc gcaggtaag 
acgaataaa tcgctcatt acttttggt ctttctttc 
caaaatttc taaaaccgg cccaatttt tactaagat 
cattttaac gttaccgga tgaggtaag acaggaaac 
tgtaagtag cgttttgtt atgcaatca aggatcttc 
aatttacat tttttaatc acacacttg ttaacgtag 
cgtcttgtc actgttatc taacacgaa gatcctttt 
tttgtgggt gcacgactc gtatcaatt gttggccct 
taccaacaa ctggtactc ttccactca cccatcctt 
actaaaaat tactgttta ggtgcgaga gcgttcagc 
aaggtcggt tccaatggg cagccttcc gcgcacttc 
caaatcctc cttatcagc ggggacccc cgactcgtt 
atcagacaa cgaaagttc tgctttccg cacgcccag 
aaaaccatc ttacaggtc aacgatgac cccaggagg 
gtctaggtc gaactcacg accggcgtc gcgaactgg 
cagcaggcc tggcagcaa acccccatc gagacatgg 
ttctaacct ctccctaga tagtacaga tgatggtac 
cccgtcgag gattccacg aaggacccc aacgtcaag 
tcgggtacc atcgacaac ttcttcttt gcggaggcc 
ccagaatgg caccgggtc ccggccgag gacacccac 
ggggctcat ttcaccagc tagtccagc cggaaaatc 
atcaggcat tccccgagc atcaagtat accaacact 
aacgtcagt taccacccc aaggagtac aagcaaacc 
acgacaagt gacaatcgg taacgctcc gacgctaag 
gacggcggc taggacacg aacaccatc cgggtctaa 
cacacccgt tccgcccgg gcaacaaac cgccgcttc 
ccgccttct acgaaaacc atcgggagc gtccgacac 
gccaacagg gcaagcgaa gaaaacaac aaaggaacg 
ggagccttt tagacttta attaattat gagattaac 
ttattccaa gattaacga atgactgac agtagggtt 
attgtgttt ttgtttaaa ttgttcata tttttgtat 
aaaagattt atttatgaa gcggccccg gaaacgcca 
gttccggag ttgaattat tgtgtgagt cacaatacg 
taactgcgt taagttgcc ataaattgc aaaattatt 
ttcgtgagg tgatttttg tattatttt ttgggtatc 
agtgggacc attttgtgg cgatttgct ttgtttagt 
ggacgttta tttgattta tcctactaa cttacttat 
tttatcctt aataattta gatactaaa gcgccgcta 
atactcgta gttgtgtga ttaccagaa acgcctttc 
ttttatatc gccagtatg aaagaacgg atcagtcaa 
tattgtaac tattactat atttaattt gttatgtat 
taaaacaat aaactaaat tttttaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY