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Gene information
General information
TC009048 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 1416029..1417483
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009048
    iBeetle screen iB_01464
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages sometimes randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages sometimesposition irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax cuticle dorsal irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009048 RA
      MPGISEMAA PDPTIPPCY VLVRKLTME EITRMTLSP 
      LFISRKCKF CRKFAYNGL ECPKCSVHY HYECALMRR 
      ECCGVRLQT ETSEQLCSK CKVCVYLGP QCSFCHRKY 
      HSSCITPYE QCCGFNLSE RPAQKPCGK CKQGVSFGP 
      RCPLCARVY HSECVENEK LVCCNVKVL KDENVNSRC 
      YKCQKRVML STPMCQFCG NFSHKKCLK FKGCCGDRR 
      PSLELFFVP SFKFMLSYF NQFDKKKAL LTNAEVRVR 
      NRTRSLRST SQDSERGRT RSMACQTPH PLDNIVITS 
      IKTADDDSD SPPVLEREN YTPPTVSSS SSRLENDDT 
      LKDFFDFLC KTIKKFPPQ LQTQVKVDL FKIVSEAEL 
      RLQRM
    CDS sequence
    • >TC009048 RA
      atgcctgga atatccgaa atggctgcc ccagatcca 
      accatccct ccatgttac gttcttgtg cggaaactg 
      accatggaa gaaatcacc cgcatgacc cttagcccc 
      cttttcata tcacgtaaa tgtaaattc tgtcgcaag 
      ttcgcatat aacggcctt gagtgcccc aaatgttca 
      gttcattac cactatgag tgtgcactg atgcgccgt 
      gaatgctgc ggggtgcgt ctccagacc gagacctcc 
      gagcaactg tgctctaaa tgtaaagtc tgtgtttat 
      ctcggcccc caatgttcc ttctgccac agaaagtac 
      cacagttct tgtattact ccatacgag cagtgctgc 
      ggcttcaat ttgtctgag cgcccggcg caaaaaccg 
      tgtgggaag tgcaaacag ggggtgtct tttgggccc 
      cgatgcccg ctttgtgcc agagtctat cattccgaa 
      tgtgttgag aatgagaag cttgtttgt tgcaatgtt 
      aaggtgctc aaagacgaa aacgttaat tctcgctgt 
      tacaaatgt cagaaaagg gtgatgctt agtacacca 
      atgtgccag ttctgcggg aatttttcc cataagaaa 
      tgtctaaaa tttaagggc tgttgtggg gacaggaga 
      ccctccctg gaactgttc ttcgttccc tctttcaag 
      tttatgttg tcgtatttt aatcaattc gataaaaag 
      aaggcacta ttgaccaat gccgaagtg agagttagg 
      aatcgcaca cgttcgtta agaagcacc tctcaagac 
      tctgaaagg ggacgaacg aggagcatg gcttgccag 
      acccctcac cctctggat aacattgtg ataactagc 
      atcaagact gctgacgac gattcggat tcaccccca 
      gttttggag agagaaaat tacacaccg ccaacggtg 
      tcatcttcg tcatctaga ttggaaaat gacgatact 
      ctcaaagac ttttttgat tttttgtgt aaaacgatt 
      aaaaagttt ccgccacaa ctgcaaaca caagttaag 
      gttgatttg tttaaaatc gtttctgaa gctgagctc 
      cggctgcaa aggatgtaa
    mRNA sequence
    • >TC009048 RA
      aaacatggt cacatggtt tgagtttgg cgggtttgg 
      tacgaaccg cctactgca tgttatctt tataaaata 
      aaaacggtt gataaaaaa taaagaaga aaacatttc 
      tccttcacc tacgcacca caaattacc acttctatg 
      cctggaata tccgaaatg gctgcccca gatccaacc 
      atccctcca tgttacgtt cttgtgcgg aaactgacc 
      atggaagaa atcacccgc atgaccctt agccccctt 
      ttcatatca cgtaaatgt aaattctgt cgcaagttc 
      gcatataac ggccttgag tgccccaaa tgttcagtt 
      cattaccac tatgagtgt gcactgatg cgccgtgaa 
      tgctgcggg gtgcgtctc cagaccgag acctccgag 
      caactgtgc tctaaatgt aaagtctgt gtttatctc 
      ggcccccaa tgttccttc tgccacaga aagtaccac 
      agttcttgt attactcca tacgagcag tgctgcggc 
      ttcaatttg tctgagcgc ccggcgcaa aaaccgtgt 
      gggaagtgc aaacagggg gtgtctttt gggccccga 
      tgcccgctt tgtgccaga gtctatcat tccgaatgt 
      gttgagaat gagaagctt gtttgttgc aatgttaag 
      gtgctcaaa gacgaaaac gttaattct cgctgttac 
      aaatgtcag aaaagggtg atgcttagt acaccaatg 
      tgccagttc tgcgggaat ttttcccat aagaaatgt 
      ctaaaattt aagggctgt tgtggggac aggagaccc 
      tccctggaa ctgttcttc gttccctct ttcaagttt 
      atgttgtcg tattttaat caattcgat aaaaagaag 
      gcactattg accaatgcc gaagtgaga gttaggaat 
      cgcacacgt tcgttaaga agcacctct caagactct 
      gaaagggga cgaacgagg agcatggct tgccagacc 
      cctcaccct ctggataac attgtgata actagcatc 
      aagactgct gacgacgat tcggattca cccccagtt 
      ttggagaga gaaaattac acaccgcca acggtgtca 
      tcttcgtca tctagattg gaaaatgac gatactctc 
      aaagacttt tttgatttt ttgtgtaaa acgattaaa 
      aagtttccg ccacaactg caaacacaa gttaaggtt 
      gatttgttt aaaatcgtt tctgaagct gagctccgg 
      ctgcaaagg atgtaatta aataccacc tgttttaag 
      tgaagaatt gtacggtgt taccacgtt tagccgaat 
      ttgtatttt ttaataaat gaatttgta atac