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Gene information
General information
TC009038 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8224581..8233768
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009038
    iBeetle screen iB_01462

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009038 RA
      MCFGCCGCC SAFRPRYKR FVDNIFPVY PQDGLVKNN 
      MEKLTFYAL SSPEKLDRI GEYMYQRAA RDIYRRRYG 
      FVIIAMEAM DQLLLACHA PALNLFVES FLKMVQKLL 
      ESTEPELQI LATQSFVKF ANIEEDTPS YHRRYDFFV 
      SKFSAMCHS SEGDQIRIA GIKGLQAVV RKTVSDDLV 
      ENIWEPVHM DKIVPSLLF NMQNARFLN NEAKEVIPE 
      DQTDPPMLA ETCLRELVG RASFGHIRA VLKPVLKHF 
      DLHKLWAPE DNPNNNEFA IHTFRIIMF SIQAQYSYT 
      VVETLVAHL DDNAKSSPI IRTSISGVL SKIIAIAAG 
      ESVGPMVLE IINSLLCHL RDSVKHGTS MTEEKQYQD 
      ALINALGEF ANHLPDYQK IEIMMFIMS KIPFPLVDN 
      HTPADNLLQ SILLKSLLK VGTKYQTIH LNSTFPVSF 
      LEPLLRMSL APDPEMRLL VQKILHTLL DRHQNLEKL 
      TKPTVNVSK LNLVLEKSS RPDLIFINK HGPIIYDAL 
      YTTLQMDSN TPDNVEAVY TTLALLCVE LASAETVID 
      LLQLVLGIQ SLALNSTTL STSQKFNLH ALVISLLVL 
      IPSVVTIPP LLEYANQIV EARTQEAPH LLPDLMVHY 
      PQDSEVANK VPHLLVDQM AVCENLKSG GIDPSRLAQ 
      TSPYGAGGP GVVIGGQRN SWIETARGS VIDINAPPE 
      VDSVSSSPG VQRKMVEEE LTFESMKKV LTESVEIRK 
      EAEARRRKQ LSDTFRTAT FSELVRRTQ PKHDVLQNK 
      LNEIFKSLS ANDRRNPTE EKKIEKPAY EQNFPELFF 
      Y
    CDS sequence
    • >TC009038 RA
      atgtgcttc ggctgctgc ggctgttgc agtgctttc 
      cggccgagg tataagaga ttcgtcgat aatattttc 
      cccgtgtac ccccaagat ggcttggtt aaaaacaac 
      atggaaaaa ctcacattc tacgccctc agctcgcca 
      gaaaagctt gacagaatt ggggagtac atgtatcag 
      agagcggcg agggatatt tatagaagg agatacggg 
      tttgttatt atcgcaatg gaagctatg gatcagctg 
      ctgttggcc tgccatgca cccgccttg aatttgttc 
      gtcgaaagc tttctaaaa atggtgcag aaattgctt 
      gaatctact gagcctgag ctgcaaatc ttggcgact 
      cagtcgttt gtaaaattc gccaacatc gaagaagac 
      accccttcc taccatcgg cgttacgac ttctttgta 
      agtaaattc tccgccatg tgccacagt agcgaaggc 
      gatcaaatc cgaattgcc ggtatcaaa ggtctccaa 
      gccgtggtc cgaaagacc gtctcggac gacctggtc 
      gaaaacatc tgggaaccg gtccacatg gacaaaatc 
      gtccctagc ttgttgttc aatatgcaa aacgctcgt 
      tttcttaat aacgaagcc aaagaggtc attcccgaa 
      gaccaaacc gacccacca atgcttgcc gagacctgt 
      cttagggaa ctcgtggga cgggcctct ttcgggcac 
      attcgagcc gttcttaag ccggttttg aaacatttc 
      gacttgcat aaactgtgg gcacccgag gataatccg 
      aataataac gaattcgcc atccacaca tttaggata 
      ataatgttt tcgatccaa gcccagtat tcttacacg 
      gtcgtggag acgctagtg gcccatttg gacgacaat 
      gccaagtct agtccgatt atccgaacg agtatttcg 
      ggggtttta tcgaaaatc attgccata gcggctggc 
      gaaagcgtg ggtccgatg gtactcgag ataattaat 
      tcgttgttg tgccacttg agagattca gtcaagcat 
      ggcacaagt atgactgaa gaaaagcaa tatcaggat 
      gcgttaatc aatgcgttg ggtgaattc gctaatcat 
      ttacccgat tatcagaaa attgaaatc atgatgttt 
      attatgagc aagattccg tttccgctt gttgacaat 
      catacaccc gccgataat ttgttgcaa agtatttta 
      ttgaagagt ttgttgaaa gttggtacc aagtaccaa 
      acgattcat ttgaattcg acgtttccg gtttcgttt 
      ttggaacct ttgttgagg atgtcgctg gcgccggac 
      cccgaaatg aggcttctg gtgcagaag attctgcac 
      acgctgctg gacaggcac cagaatttg gagaagttg 
      accaaaccg acagtgaac gtgagcaaa ctaaacctc 
      gtattggaa aaatcgtct cgccccgac ctaattttc 
      atcaacaaa cacggcccc atcatttac gacgctctc 
      tacaccact ctccagatg gactccaac acgcccgac 
      aacgtggag gcggtctac acgacgctg gcattgtta 
      tgcgtcgaa ctggcgagc gctgagacg gttatcgac 
      ttattgcag ttagtctta gggatccaa agtcttgcc 
      ttgaacagc acgaccctc tccacatcg caaaaattc 
      aatctccac gctttggtg atcagtctt ctggtcctc 
      atccccagc gtggtgact attccccca ttgttggag 
      tacgctaat cagatcgtg gaggcgagg acgcaagag 
      gcgccgcat ttactcccc gacttaatg gtgcactac 
      ccccaagat agcgaagtg gcgaataaa gtgccgcat 
      cttctggtc gatcaaatg gctgtgtgt gagaatctc 
      aagagtgga gggatagat ccgtcaagg ttggctcaa 
      acgtcgccg tatggggct ggagggccg ggggttgtg 
      attggagga cagaggaac agctggatc gagacggct 
      aggggcagt gttattgat attaatgcg ccgccagag 
      gtcgatagc gtttccagt agtcctgga gttcagagg 
      aaaatggtc gaagaggag ttaactttt gaaagtatg 
      aagaaagta ttgaccgaa tcggtggaa attcggaaa 
      gaggcggaa gcacgccgg cgcaaacaa ttgagtgac 
      actttccgg acagccact ttttcggaa cttgtcagg 
      cggactcag cccaaacat gatgtatta cagaataag 
      ttgaatgaa attttcaag tccctcagt gcgaacgac 
      cgacgcaac ccgaccgaa gaaaagaaa atcgagaaa 
      ccagcgtat gaacaaaat ttccccgag ttgttcttc 
      tattga
    mRNA sequence
    • >TC009038 RA
      agtcgatca aatcaaaca agtttccat catgtatgg 
      gatcaactt tgctctaat taaacgttt cgttctaat 
      acacatcat aatagcagt aatttaatt aaaagaaaa 
      ccgccagtt ataaagcta caaccttcg catccttaa 
      ttacccacc ttaatgtta gctttaaca acgcaaggt 
      ccacaaaat atgttgcta aacaagttg ttccagtga 
      attttttga gcagttttt tgaataatc aagtttgta 
      aaaatgtcg ttcattaag tgttgcttt gacgggatt 
      gaaattgcc gaagtcttg gatactttt gcccagaaa 
      tgcacggac ccgggctat gccgccact ttgacagct 
      gttgacact tgtcaataa catacccat atttcaatc 
      aatttttac tttttacta tagataaac acgcatata 
      atgtgcttc ggctgctgc ggctgttgc agtgctttc 
      cggccgagg tataagaga ttcgtcgat aatattttc 
      cccgtgtac ccccaagat ggcttggtt aaaaacaac 
      atggaaaaa ctcacattc tacgccctc agctcgcca 
      gaaaagctt gacagaatt ggggagtac atgtatcag 
      agagcggcg agggatatt tatagaagg agatacggg 
      tttgttatt atcgcaatg gaagctatg gatcagctg 
      ctgttggcc tgccatgca cccgccttg aatttgttc 
      gtcgaaagc tttctaaaa atggtgcag aaattgctt 
      gaatctact gagcctgag ctgcaaatc ttggcgact 
      cagtcgttt gtaaaattc gccaacatc gaagaagac 
      accccttcc taccatcgg cgttacgac ttctttgta 
      agtaaattc tccgccatg tgccacagt agcgaaggc 
      gatcaaatc cgaattgcc ggtatcaaa ggtctccaa 
      gccgtggtc cgaaagacc gtctcggac gacctggtc 
      gaaaacatc tgggaaccg gtccacatg gacaaaatc 
      gtccctagc ttgttgttc aatatgcaa aacgctcgt 
      tttcttaat aacgaagcc aaagaggtc attcccgaa 
      gaccaaacc gacccacca atgcttgcc gagacctgt 
      cttagggaa ctcgtggga cgggcctct ttcgggcac 
      attcgagcc gttcttaag ccggttttg aaacatttc 
      gacttgcat aaactgtgg gcacccgag gataatccg 
      aataataac gaattcgcc atccacaca tttaggata 
      ataatgttt tcgatccaa gcccagtat tcttacacg 
      gtcgtggag acgctagtg gcccatttg gacgacaat 
      gccaagtct agtccgatt atccgaacg agtatttcg 
      ggggtttta tcgaaaatc attgccata gcggctggc 
      gaaagcgtg ggtccgatg gtactcgag ataattaat 
      tcgttgttg tgccacttg agagattca gtcaagcat 
      ggcacaagt atgactgaa gaaaagcaa tatcaggat 
      gcgttaatc aatgcgttg ggtgaattc gctaatcat 
      ttacccgat tatcagaaa attgaaatc atgatgttt 
      attatgagc aagattccg tttccgctt gttgacaat 
      catacaccc gccgataat ttgttgcaa agtatttta 
      ttgaagagt ttgttgaaa gttggtacc aagtaccaa 
      acgattcat ttgaattcg acgtttccg gtttcgttt 
      ttggaacct ttgttgagg atgtcgctg gcgccggac 
      cccgaaatg aggcttctg gtgcagaag attctgcac 
      acgctgctg gacaggcac cagaatttg gagaagttg 
      accaaaccg acagtgaac gtgagcaaa ctaaacctc 
      gtattggaa aaatcgtct cgccccgac ctaattttc 
      atcaacaaa cacggcccc atcatttac gacgctctc 
      tacaccact ctccagatg gactccaac acgcccgac 
      aacgtggag gcggtctac acgacgctg gcattgtta 
      tgcgtcgaa ctggcgagc gctgagacg gttatcgac 
      ttattgcag ttagtctta gggatccaa agtcttgcc 
      ttgaacagc acgaccctc tccacatcg caaaaattc 
      aatctccac gctttggtg atcagtctt ctggtcctc 
      atccccagc gtggtgact attccccca ttgttggag 
      tacgctaat cagatcgtg gaggcgagg acgcaagag 
      gcgccgcat ttactcccc gacttaatg gtgcactac 
      ccccaagat agcgaagtg gcgaataaa gtgccgcat 
      cttctggtc gatcaaatg gctgtgtgt gagaatctc 
      aagagtgga gggatagat ccgtcaagg ttggctcaa 
      acgtcgccg tatggggct ggagggccg ggggttgtg 
      attggagga cagaggaac agctggatc gagacggct 
      aggggcagt gttattgat attaatgcg ccgccagag 
      gtcgatagc gtttccagt agtcctgga gttcagagg 
      aaaatggtc gaagaggag ttaactttt gaaagtatg 
      aagaaagta ttgaccgaa tcggtggaa attcggaaa 
      gaggcggaa gcacgccgg cgcaaacaa ttgagtgac 
      actttccgg acagccact ttttcggaa cttgtcagg 
      cggactcag cccaaacat gatgtatta cagaataag 
      ttgaatgaa attttcaag tccctcagt gcgaacgac 
      cgacgcaac ccgaccgaa gaaaagaaa atcgagaaa 
      ccagcgtat gaacaaaat ttccccgag ttgttcttc 
      tattgaatt tttccgctt agcaatact ccttagtgt 
      ctgttattg tttatggaa agtttataa gtatgttac 
      gtatttttc tagagaggt tctaatgat gtttgagag 
      gtgaaaaaa tatacgatt attcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY