| General information | |||
| TC009038 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 8224581..8233768 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009038 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01462 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
adult nutritional state - Number of animals showing the phenotype: 4 (11 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC009038 RA
MCFGCCGCC SAFRPRYKR FVDNIFPVY PQDGLVKNN MEKLTFYAL SSPEKLDRI GEYMYQRAA RDIYRRRYG FVIIAMEAM DQLLLACHA PALNLFVES FLKMVQKLL ESTEPELQI LATQSFVKF ANIEEDTPS YHRRYDFFV SKFSAMCHS SEGDQIRIA GIKGLQAVV RKTVSDDLV ENIWEPVHM DKIVPSLLF NMQNARFLN NEAKEVIPE DQTDPPMLA ETCLRELVG RASFGHIRA VLKPVLKHF DLHKLWAPE DNPNNNEFA IHTFRIIMF SIQAQYSYT VVETLVAHL DDNAKSSPI IRTSISGVL SKIIAIAAG ESVGPMVLE IINSLLCHL RDSVKHGTS MTEEKQYQD ALINALGEF ANHLPDYQK IEIMMFIMS KIPFPLVDN HTPADNLLQ SILLKSLLK VGTKYQTIH LNSTFPVSF LEPLLRMSL APDPEMRLL VQKILHTLL DRHQNLEKL TKPTVNVSK LNLVLEKSS RPDLIFINK HGPIIYDAL YTTLQMDSN TPDNVEAVY TTLALLCVE LASAETVID LLQLVLGIQ SLALNSTTL STSQKFNLH ALVISLLVL IPSVVTIPP LLEYANQIV EARTQEAPH LLPDLMVHY PQDSEVANK VPHLLVDQM AVCENLKSG GIDPSRLAQ TSPYGAGGP GVVIGGQRN SWIETARGS VIDINAPPE VDSVSSSPG VQRKMVEEE LTFESMKKV LTESVEIRK EAEARRRKQ LSDTFRTAT FSELVRRTQ PKHDVLQNK LNEIFKSLS ANDRRNPTE EKKIEKPAY EQNFPELFF Y
- >TC009038 RA
atgtgcttc ggctgctgc ggctgttgc agtgctttc cggccgagg tataagaga ttcgtcgat aatattttc cccgtgtac ccccaagat ggcttggtt aaaaacaac atggaaaaa ctcacattc tacgccctc agctcgcca gaaaagctt gacagaatt ggggagtac atgtatcag agagcggcg agggatatt tatagaagg agatacggg tttgttatt atcgcaatg gaagctatg gatcagctg ctgttggcc tgccatgca cccgccttg aatttgttc gtcgaaagc tttctaaaa atggtgcag aaattgctt gaatctact gagcctgag ctgcaaatc ttggcgact cagtcgttt gtaaaattc gccaacatc gaagaagac accccttcc taccatcgg cgttacgac ttctttgta agtaaattc tccgccatg tgccacagt agcgaaggc gatcaaatc cgaattgcc ggtatcaaa ggtctccaa gccgtggtc cgaaagacc gtctcggac gacctggtc gaaaacatc tgggaaccg gtccacatg gacaaaatc gtccctagc ttgttgttc aatatgcaa aacgctcgt tttcttaat aacgaagcc aaagaggtc attcccgaa gaccaaacc gacccacca atgcttgcc gagacctgt cttagggaa ctcgtggga cgggcctct ttcgggcac attcgagcc gttcttaag ccggttttg aaacatttc gacttgcat aaactgtgg gcacccgag gataatccg aataataac gaattcgcc atccacaca tttaggata ataatgttt tcgatccaa gcccagtat tcttacacg gtcgtggag acgctagtg gcccatttg gacgacaat gccaagtct agtccgatt atccgaacg agtatttcg ggggtttta tcgaaaatc attgccata gcggctggc gaaagcgtg ggtccgatg gtactcgag ataattaat tcgttgttg tgccacttg agagattca gtcaagcat ggcacaagt atgactgaa gaaaagcaa tatcaggat gcgttaatc aatgcgttg ggtgaattc gctaatcat ttacccgat tatcagaaa attgaaatc atgatgttt attatgagc aagattccg tttccgctt gttgacaat catacaccc gccgataat ttgttgcaa agtatttta ttgaagagt ttgttgaaa gttggtacc aagtaccaa acgattcat ttgaattcg acgtttccg gtttcgttt ttggaacct ttgttgagg atgtcgctg gcgccggac cccgaaatg aggcttctg gtgcagaag attctgcac acgctgctg gacaggcac cagaatttg gagaagttg accaaaccg acagtgaac gtgagcaaa ctaaacctc gtattggaa aaatcgtct cgccccgac ctaattttc atcaacaaa cacggcccc atcatttac gacgctctc tacaccact ctccagatg gactccaac acgcccgac aacgtggag gcggtctac acgacgctg gcattgtta tgcgtcgaa ctggcgagc gctgagacg gttatcgac ttattgcag ttagtctta gggatccaa agtcttgcc ttgaacagc acgaccctc tccacatcg caaaaattc aatctccac gctttggtg atcagtctt ctggtcctc atccccagc gtggtgact attccccca ttgttggag tacgctaat cagatcgtg gaggcgagg acgcaagag gcgccgcat ttactcccc gacttaatg gtgcactac ccccaagat agcgaagtg gcgaataaa gtgccgcat cttctggtc gatcaaatg gctgtgtgt gagaatctc aagagtgga gggatagat ccgtcaagg ttggctcaa acgtcgccg tatggggct ggagggccg ggggttgtg attggagga cagaggaac agctggatc gagacggct aggggcagt gttattgat attaatgcg ccgccagag gtcgatagc gtttccagt agtcctgga gttcagagg aaaatggtc gaagaggag ttaactttt gaaagtatg aagaaagta ttgaccgaa tcggtggaa attcggaaa gaggcggaa gcacgccgg cgcaaacaa ttgagtgac actttccgg acagccact ttttcggaa cttgtcagg cggactcag cccaaacat gatgtatta cagaataag ttgaatgaa attttcaag tccctcagt gcgaacgac cgacgcaac ccgaccgaa gaaaagaaa atcgagaaa ccagcgtat gaacaaaat ttccccgag ttgttcttc tattga
- >TC009038 RA
agtcgatca aatcaaaca agtttccat catgtatgg gatcaactt tgctctaat taaacgttt cgttctaat acacatcat aatagcagt aatttaatt aaaagaaaa ccgccagtt ataaagcta caaccttcg catccttaa ttacccacc ttaatgtta gctttaaca acgcaaggt ccacaaaat atgttgcta aacaagttg ttccagtga attttttga gcagttttt tgaataatc aagtttgta aaaatgtcg ttcattaag tgttgcttt gacgggatt gaaattgcc gaagtcttg gatactttt gcccagaaa tgcacggac ccgggctat gccgccact ttgacagct gttgacact tgtcaataa catacccat atttcaatc aatttttac tttttacta tagataaac acgcatata atgtgcttc ggctgctgc ggctgttgc agtgctttc cggccgagg tataagaga ttcgtcgat aatattttc cccgtgtac ccccaagat ggcttggtt aaaaacaac atggaaaaa ctcacattc tacgccctc agctcgcca gaaaagctt gacagaatt ggggagtac atgtatcag agagcggcg agggatatt tatagaagg agatacggg tttgttatt atcgcaatg gaagctatg gatcagctg ctgttggcc tgccatgca cccgccttg aatttgttc gtcgaaagc tttctaaaa atggtgcag aaattgctt gaatctact gagcctgag ctgcaaatc ttggcgact cagtcgttt gtaaaattc gccaacatc gaagaagac accccttcc taccatcgg cgttacgac ttctttgta agtaaattc tccgccatg tgccacagt agcgaaggc gatcaaatc cgaattgcc ggtatcaaa ggtctccaa gccgtggtc cgaaagacc gtctcggac gacctggtc gaaaacatc tgggaaccg gtccacatg gacaaaatc gtccctagc ttgttgttc aatatgcaa aacgctcgt tttcttaat aacgaagcc aaagaggtc attcccgaa gaccaaacc gacccacca atgcttgcc gagacctgt cttagggaa ctcgtggga cgggcctct ttcgggcac attcgagcc gttcttaag ccggttttg aaacatttc gacttgcat aaactgtgg gcacccgag gataatccg aataataac gaattcgcc atccacaca tttaggata ataatgttt tcgatccaa gcccagtat tcttacacg gtcgtggag acgctagtg gcccatttg gacgacaat gccaagtct agtccgatt atccgaacg agtatttcg ggggtttta tcgaaaatc attgccata gcggctggc gaaagcgtg ggtccgatg gtactcgag ataattaat tcgttgttg tgccacttg agagattca gtcaagcat ggcacaagt atgactgaa gaaaagcaa tatcaggat gcgttaatc aatgcgttg ggtgaattc gctaatcat ttacccgat tatcagaaa attgaaatc atgatgttt attatgagc aagattccg tttccgctt gttgacaat catacaccc gccgataat ttgttgcaa agtatttta ttgaagagt ttgttgaaa gttggtacc aagtaccaa acgattcat ttgaattcg acgtttccg gtttcgttt ttggaacct ttgttgagg atgtcgctg gcgccggac cccgaaatg aggcttctg gtgcagaag attctgcac acgctgctg gacaggcac cagaatttg gagaagttg accaaaccg acagtgaac gtgagcaaa ctaaacctc gtattggaa aaatcgtct cgccccgac ctaattttc atcaacaaa cacggcccc atcatttac gacgctctc tacaccact ctccagatg gactccaac acgcccgac aacgtggag gcggtctac acgacgctg gcattgtta tgcgtcgaa ctggcgagc gctgagacg gttatcgac ttattgcag ttagtctta gggatccaa agtcttgcc ttgaacagc acgaccctc tccacatcg caaaaattc aatctccac gctttggtg atcagtctt ctggtcctc atccccagc gtggtgact attccccca ttgttggag tacgctaat cagatcgtg gaggcgagg acgcaagag gcgccgcat ttactcccc gacttaatg gtgcactac ccccaagat agcgaagtg gcgaataaa gtgccgcat cttctggtc gatcaaatg gctgtgtgt gagaatctc aagagtgga gggatagat ccgtcaagg ttggctcaa acgtcgccg tatggggct ggagggccg ggggttgtg attggagga cagaggaac agctggatc gagacggct aggggcagt gttattgat attaatgcg ccgccagag gtcgatagc gtttccagt agtcctgga gttcagagg aaaatggtc gaagaggag ttaactttt gaaagtatg aagaaagta ttgaccgaa tcggtggaa attcggaaa gaggcggaa gcacgccgg cgcaaacaa ttgagtgac actttccgg acagccact ttttcggaa cttgtcagg cggactcag cccaaacat gatgtatta cagaataag ttgaatgaa attttcaag tccctcagt gcgaacgac cgacgcaac ccgaccgaa gaaaagaaa atcgagaaa ccagcgtat gaacaaaat ttccccgag ttgttcttc tattgaatt tttccgctt agcaatact ccttagtgt ctgttattg tttatggaa agtttataa gtatgttac gtatttttc tagagaggt tctaatgat gtttgagag gtgaaaaaa tatacgatt attcc
