Status
Please wait ...
Gene information
General information
TC009035 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8322587..8324358
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009035
    iBeetle screen iB_01461

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4
    mature egg complete not present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Pupal morphology
    wings blistered - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009035 RA
      MREIVHIQA GQCGNQIGA KFWEVISDE HGIDPTGTY 
      HGDSDLQLE RINVYYNEA TGGKYVPRA ILVDLEPGT 
      MDSVRSGPF GQIFRPDNF VFGQSGAGN NWAKGHYTE 
      GAELVDSVL DVVRKEAEG CDCMQGFQL THSLGGGTG 
      SGLGTLLIS KIREEYPDR IMNTFSVVP SPKVSDTVV 
      EPYNATLSV HQLVENTDE TYCIDNEAL YDICFRTLK 
      LTTPTYGDL NHLVSATMS GVTTCLRFP GQLNSDLRK 
      LAVNMVPFP RLHFFMPGF APLTSRGSQ QYRALTVPE 
      LVMQMFDAK NMMAACDPR HGRYLTVAA IFRGRMSMK 
      EVDEQMLNI QNKNSSYFV EWIPNNVKT AVCDIPPRG 
      LKMSSTFIG NSTCIQELF KRISEQFTA MFRRKAFLH 
      WYTGEGMDE MEFTEAESN MNDLVAEYQ QYQDATAEE 
      EGEFDEEEE GDNEGEN
    CDS sequence
    • >TC009035 RA
      atgcgagaa atagtccac atacaagca ggtcaatgc 
      ggcaatcaa ataggagca aagttttgg gaagtgatc 
      tcagatgag catggaatc gaccctacg gggacatat 
      cacggggat tcggacctc cagctggag cgaatcaac 
      gtctactac aatgaggcg accggcggg aaatatgtc 
      cctcgcgcc atcctcgta gacctggag cccggcacc 
      atggactcg gtcaggtcc ggtcccttc ggccagatt 
      ttccggccg gacaacttc gttttcggc cagtccgga 
      gccgggaac aactgggcc aagggccac tacaccgaa 
      ggggccgag ctggtggac tcggtgctg gacgtggtg 
      cgcaaggag gccgagggc tgcgactgc atgcagggc 
      ttccagctg acgcactcg ctgggaggg ggcaccggc 
      tcggggctg ggcaccctc ctcatttcg aaaatcaga 
      gaggagtac cccgatcgc atcatgaac accttctcc 
      gtagtgccg tccccgaag gtctccgac actgtcgtg 
      gagccctac aatgccacc ctctccgta catcagctc 
      gtagaaaac acagacgag acgtactgc atcgacaat 
      gaggctctc tacgacatc tgcttcagg actctcaaa 
      ctcacgacc cccacctac ggggatttg aaccacctg 
      gtgtcggca accatgtct ggggtcacc acttgtctc 
      cgcttcccg ggccagctg aactcagat ttgaggaaa 
      ttggccgtg aatatggtg ccgttcccc cgactccac 
      ttctttatg ccgggcttt gcccccctg acttccaga 
      gggagccag caatatcgg gctttaacc gtccccgag 
      ttggtcatg cagatgttc gacgctaag aacatgatg 
      gccgcttgc gaccccaga catgggcgc tacttaacc 
      gtggccgcc atcttcaga ggaagaatg tccatgaag 
      gaggtggac gagcagatg ctcaatatc cagaacaag 
      aacagcagc tactttgtc gagtggatc cccaacaac 
      gtcaagacc gctgtgtgt gacattccc cctcgcggc 
      ttaaaaatg tcgtcgact tttattgga aattctaca 
      tgtattcaa gaattgttt aagaggatt tcggaacag 
      ttcaccgca atgtttaga agaaaggca tttttgcat 
      tggtacact ggcgaaggc atggacgaa atggagttt 
      actgaggct gagagcaac atgaatgat ttagttgcg 
      gagtaccaa cagtaccaa gacgccacc gccgaagag 
      gaaggagaa tttgacgag gaagaagaa ggcgacaac 
      gaaggagaa aattaa
    mRNA sequence
    • >TC009035 RA
      acgcgaatt taaaattcc gaaagaggc ggcagtttt 
      gaaacaaca tctgctaca tggttacga gtttgttta 
      ctccaattt tcggtattt ctttaataa atttgttta 
      acgttaaag taaaatgcg agaaatagt ccacataca 
      agcaggtca atgcggcaa tcaaatagg agcaaagtt 
      ttgggaagt gatctcaga tgagcatgg aatcgaccc 
      tacggggac atatcacgg ggattcgga cctccagct 
      ggagcgaat caacgtcta ctacaatga ggcgaccgg 
      cgggaaata tgtccctcg cgccatcct cgtagacct 
      ggagcccgg caccatgga ctcggtcag gtccggtcc 
      cttcggcca gattttccg gccggacaa cttcgtttt 
      cggccagtc cggagccgg gaacaactg ggccaaggg 
      ccactacac cgaaggggc cgagctggt ggactcggt 
      gctggacgt ggtgcgcaa ggaggccga gggctgcga 
      ctgcatgca gggcttcca gctgacgca ctcgctggg 
      agggggcac cggctcggg gctgggcac cctcctcat 
      ttcgaaaat cagagagga gtaccccga tcgcatcat 
      gaacacctt ctccgtagt gccgtcccc gaaggtctc 
      cgacactgt cgtggagcc ctacaatgc caccctctc 
      cgtacatca gctcgtaga aaacacaga cgagacgta 
      ctgcatcga caatgaggc tctctacga catctgctt 
      caggactct caaactcac gacccccac ctacgggga 
      tttgaacca cctggtgtc ggcaaccat gtctggggt 
      caccacttg tctccgctt cccgggcca gctgaactc 
      agatttgag gaaattggc cgtgaatat ggtgccgtt 
      cccccgact ccacttctt tatgccggg ctttgcccc 
      cctgacttc cagagggag ccagcaata tcgggcttt 
      aaccgtccc cgagttggt catgcagat gttcgacgc 
      taagaacat gatggccgc ttgcgaccc cagacatgg 
      gcgctactt aaccgtggc cgccatctt cagaggaag 
      aatgtccat gaaggaggt ggacgagca gatgctcaa 
      tatccagaa caagaacag cagctactt tgtcgagtg 
      gatccccaa caacgtcaa gaccgctgt gtgtgacat 
      tccccctcg cggcttaaa aatgtcgtc gacttttat 
      tggaaattc tacatgtat tcaagaatt gtttaagag 
      gatttcgga acagttcac cgcaatgtt tagaagaaa 
      ggcattttt gcattggta cactggcga aggcatgga 
      cgaaatgga gtttactga ggctgagag caacatgaa 
      tgatttagt tgcggagta ccaacagta ccaagacgc 
      caccgccga agaggaagg agaatttga cgaggaaga 
      agaaggcga caacgaagg agaaaatta accgatttt 
      tgaaaattt gcaagttga acggtgtat tggaccgat 
      ttatgatgt tatattgta ttttttgta atagatagt 
      aaatttttc aacaaattt ttcaattaa ctacactta 
      taaattcta aattaaatt tcgtgggca tcaaattgg 
      ttttcctct ctagaataa ataaggaac ttaattact 
      accccaaat gaaaaggca ttgggtttt tgatatttg 
      cgggttgtt gcataaata aagttataa cgaacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY